研究者詳細 - 島内　寿徳

J-GLOBAL

蛍光分子封入リポソームアレイセンサを用いたターゲットタンパク質の検出

Imamura Ryota, Zhang Ziyang, Murata Naoki, Yamashita Kaoru, Fukuzawa Masayuki, Noda Minoru, Shimanouchi Toshinori

「センサ・マイクロマシンと応用システム」シンポジウム論文集電気学会センサ・マイクロマシン部門 [編] 32 1 - 4 2015年10月

木質バイオマスの亜臨界水抽出と撥水性界面形成への応用招待

島内寿徳, 神庭朋也, Wei Yang, 木村幸敬

ケミカルエンジニヤリング 59 ( 7 ) 66 - 71 2014年9月

“Bio‐Inspired”の視点から膜学を考える:生体膜と人工膜のはざま Membranomeを基盤とするBio‐Inspired膜へのアプローチ

馬越大, 島内寿徳, 菅恵嗣

膜 37 ( 6 ) 264 - 269 2012年11月

記述言語：日本語出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

It is important to establish a methodology to design and develop a "Bio-Inspired Membrane", which can be defined as an "Artificial Membrane" inspired by structures and functions observed in "Biomembrane". Several approaches relating to this research area are reviewed based on a hierarchical view about the structures and characteristics of the above membranes. A "Membranome" research utilizing a gel membrane immobilizing liposome membrane at high concentration is herewith introduced as one of several approaches to create the "Bio-Inspired Membrane". Some possible data required for the "Bio-Inspired Membrane" are finally discussed, focusing on the physicochemical property of self-assembly included in a total system.

DOI： 10.5360/membrane.37.264

ポスターセッション (Poster Session)

吉宗美紀, 熊切泉, 馬越大, 島内寿徳

膜 37 ( 6 ) 301 - 303 2012年11月

Leakage Current Microsensor Using Liposome Entrapping Electrolyte for Detection of Target Protein

TAKADA Keisuke, OHARA Yuri, YAMASHITA Kaoru, NODA Minoru, SHIMANOUCHI Toshinori, UMAKOSHI Hiroshi

電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan 2012 ( 8 ) 15 - 19 2012年6月

人工細胞膜上におけるアミロイド形成招待査読

島内寿徳, 北浦奈知, 馬越大, 久保井亮一

表面科学 33 40 - 46 2012年

生体膜晶析工学の創成に関する基礎工学的研究招待

島内寿徳

膜 36 233 - 239 2011年

New Planar-Type Leakage Current and Impedance Microsensor for Detection of Interaction between Electrolyte-Entrapping Liposome and Protein

P. Lorchirachoonkul, I. Goto, T. Shimanouchi, K. Yamashita, M. Noda

EUROSENSORS XXV 25 1449 - 1452 2011年

記述言語：英語出版者・発行元：ELSEVIER SCIENCE BV

We have developed a new leakage current microsensor by using simpler planar processes than Si-surface-bulk micromachining processes used in the previous microwell structure. This sensor fabrication and structure can easily make a target solution volume smaller than mu L with excellent immobilization of the droplet and intact biomolecules as sensing elements, as a result, reduce effectively the background noise current in the microsensor and improve reproducibility of the results. The leakage current due to the biochemical interaction was successfully evaluated, dependent on the droplet protein concentration. Cole-Cole plots from the impedance analysis also show quantitative difference between with and without the interaction, depending on the charge-transfer impedance that results from the condition and structure of liposome and lipid membrane after the interaction. (C) 2011 Published by Elsevier Ltd.

DOI： 10.1016/j.proeng.2011.12.358

Web of Science

Scopus

Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes

P. Lorchirachoonkul, T. Shimanouchi, K. Yamashita, H. Umakoshi, R. Kuboi, M. Noda

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 3 1982 - 1984 2010年12月

We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.

Scopus

生体膜界面を晶析場とするタンパク質のアミロイド形成/多形の制御招待

As-Lipozyme: Characterization of Its Activity Like Antioxidative Enzymes

L. Q. Tuan, T.T.T. Huong, H. Umakoshi, T. Shimanouchi, R. Kuboi, K. Shiomori, Y. Baba

Proc. 2nd International Symposium on Health Hazards of Arsenic Contamination 138 - 142 2010年5月

島内寿徳, 北浦奈知, 大西諒, 馬越大, 久保井亮一

分離技術 40 ( 6 ) 455 - 462 2010年

脂質膜界面特性に基づくベシクル形成制御

島内寿徳, 馬越大, 久保井亮一, 石井治之, 吉本則子

化学工学シンポジウムシリーズ 81 107 - 114 2010年

2P-1101 リポソーム膜によるアミロイドの多形制御(2bタンパク質工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)

島内寿徳, 大西諒, 北浦奈知, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 22 34 - 34 2010年

MN-P26 Development of membrane chip system for study on membrane-protein interaction(Section X Micro/Nano Technology for Analysis and Cell Manipulation)

Shimanouchi Toshinori, Ishii Haruyuki, Oyama Ena, Shimauchi Naoya, Umakoshi Hiroshi, Kuboi Ryoichi

Journal of bioscience and bioengineering 108 ( 1 ) S162 - S163 2009年11月

BE-P3 Preparation of liposome immobilized membrane module and its application(Section V Biomolecular Engineering and Bioseparation)

Umakoshi Hiroshi, Sugaya Hiroyuki, Tohtake Yuji, Shimanouchi Toshinori, Kuboi Ryoichi

Journal of bioscience and bioengineering 108 ( 1 ) S65 2009年11月

漏れ電流マイクロセンサを用いた電解質封入リポソーム・タンパク質間相互作用センシング

有延康, 浅井健, 山下馨, 野田実, 島内寿徳, 馬越大, 奥山雅則, 久保井亮一

電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan 2009 ( 8 ) 57 - 60 2009年7月

メンブレン・ストレスバイオテクノロジーとアルツハイマー病の診断

馬越大, 島内寿徳, 久保井亮一

分離技術 39 ( 2 ) 109 - 114 2009年3月

LIPOzyme: Basic and Its Possible Application

2009年

Immobilization of Liposome on Solid Surface: A Quartz Microbalance Study

J. Colloid Surface Interf. in press (2009) 2009年

Negatively-Charged Liposome is Potent Inhibitor of Protein Folding in Post-Translational Process of in vitro Gene Expression

Biochem. Eng. J. in press (2009) 2009年

1Ep18 アミロイド性タンパク質の表面特性解析 : メンブレンチップ解析の利用(その1)(タンパク質工学,一般講演)

島内寿徳, Huong Thi Vu, 嶋内直哉, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 21 56 - 56 2009年

2Fp06 酵母の凝集現象に関わる細胞表層因子の解析(発酵生理学・発酵工学,一般講演)

浅山知大, 島内寿徳, 馬越大, 久保井亮一, 立花太郎, 東雅之

日本生物工学会大会講演要旨集 21 78 - 78 2009年

1Ep20 脂質膜上におけるアミロイド形成と多形(その3)(タンパク質工学,一般講演)

島内寿徳, 嶋内直哉, 大西諒, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 21 57 - 57 2009年

1Ep19 メンブレンチップによる脂質膜上におけるアミロイド形成挙動のモニタリング(その2)(タンパク質工学,一般講演)

島内寿徳, Huong Thi Vu, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 21 56 - 56 2009年

微量リポソームPt表面固定化マイクロボロメータによる熱化学センサ

浅井健, 山下馨, 野田実, 島内寿徳, 奥山雅則, 久保井亮一

電気学会研究会資料. CHS, ケミカルセンサ研究会 2008 ( 1 ) 31 - 34 2008年6月

メンブレン・ストレスバイオテクノロジーの新展開

久保井亮一, 馬越大, 島内寿徳

膜 33(6), 300-306 (2008) ( 6 ) 300 - 306 2008年

記述言語：日本語出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

A "Biomembrane"is a key interface to integrate production and separation of target materials through the creationof "new order"on its surface. A model biomembrane, liposome, can recognize (separate)the (bio)moleculesthrough (i)electrostatic, (ii)hydrophobic interaction, and (iii)stabilization effect of hydrogen bonds in the hydropho-bic lipid environment. A novel biomembrane-based catalysis (LIPOzyme: liposome + enzyme)can be designed anddeveloped by integrating the above recognition sites and a simple catalytic center on the liposome surface. A processdesign utilizing LIPOzyme chemistry and the recognition (bioseparation)functions of model-biomembrane (lipo-some)is the basic strategy to achieve a "Biomembrane Process Chemistry"as an application of "Membranomics".

DOI： 10.5360/membrane.33.300

その他リンク： https://jlc.jst.go.jp/DN/JALC/00322917093?from=CiNii

メンブレン・ストレスバイオテクノロジーの新展開

久保井亮一, 馬越大, 島内寿徳

膜 33(6), 300-306 (2008) ( 6 ) 300 - 306 2008年

記述言語：日本語出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

DOI： 10.5360/membrane.33.300

IMMOBILIZED-LIPOSOME SENSOR SYSTEM FOR DETECTION OF DAMAGED PROTEINS

その他リンク： https://jlc.jst.go.jp/DN/JALC/00322917093?from=CiNii

Immobilized Liposome Chromatography/Bio-Membrane Module for Bioseparation and Bioreactor

2008年

LIPOzyme Design Based on Molecular Recognition Function of Liposome -Biomembrane Process Chemistry and Separation Technology-

2008年

Separation and Analysis for Aβ Peptides Based on Recognition Function of Abnormal Biomembrane ~Membrane Chip Analysis Can Brighten a Potential Aspect of Alzheimer’s Disease~

2008年

Cationic Liposome DOTAP can Knock-out mRNA and Silence Its Translation in E.coli Cell Free Translation System

2008年

Simple Ligand/Porphyrin Complex can Induce Both SOD and POD Activities Similar to Those of Metal-Ligand Complex

2008年

Preparation and Properties of Antioxidative LIPOzyme

2008年

Biomembrane Process Chemistry and Separation Technology~ Design and Development of Antioxidative LIPOzyme in One-Pot Based on Molecular Recognition Function of Liposome

2008年

LIPOzyme design based on molecular recognition function of liposome

2008年

Amyloid fibril formation on biomembrane: Analysis using membrane chip system

2008年

Characterization of Surface Properties of Streptomyces griseus Cell after Heat Treatment with Liposome Using Aqueous Two-Phase Method

2008年

Kuboi R., Morita S., V. T. Huong, Ishii H., Shimanouchi T., Umakoshi H.

Annual Report of FY 2007, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST) 120 - 124 2008年

Liposome-Assisted Refolding of Microbial Transglutaminase

Umakoshi Hiroshi, Yoshimoto Noriko, Yoshimoto Makoto, SHIMANOUCHI Toshinori, KUBOI Ryoichi

膜 32 ( 5 ) 287 - 293 2007年9月

固定化リポソームセンサーと膜関連疾病への応用

久保井亮一, 馬越大, 島内寿徳

膜 32(1), 32-39 ( 1 ) 32 - 39 2007年

記述言語：日本語出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

The diseases caused by the membrane disorder or damages are categorized as the Membrane-related diseases. Alzheimer's disease (AD), which is one of the membrane-related diseases, has been considered to be accompanied by the oxidative damage of the neuronal cell membrane. Amyloid β-peptide (Aβ), which is a pathological key peptide of AD, interacts with biomembrane and this interaction has been recently paid attention from the viewpoints of not only conformational abnormality but also the influence to biomembrane. And therefore, the modeling of biomembrane using liposome has been desired to develop the liposome-based sensor system. In this article, we surveyed the sensor system using liposome. Firstly, the immobilization techniques for preparation of the immobilized-liposome sensor are surveyed. Secondary, some kinds of sensor have been introduced: (i) (metal affinity) immobilized-liposome chromatography, (ii) immobilized-liposome quartz crystal microbalance method, (iii) immobilized-liposome sensor (Amperometric method), (iv) membrane chip system. These techniques have been developed in a framework of "Membrane Stress Biotechnology". The above methodologies could provide us the quantitative properties of stress responsive function of both the protein and the membrane.

DOI： 10.5360/membrane.32.32

その他リンク： https://jlc.jst.go.jp/DN/JALC/00289812370?from=CiNii

固定化リポソームセンサーと膜関連疾病への応用招待

久保井亮一, 馬越大, 島内寿徳

膜 32 ( 1 ) 32 - 39 2007年

記述言語：日本語掲載種別：記事・総説・解説・論説等（学術雑誌）出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

DOI： 10.5360/membrane.32.32

DESIGN AND DEVELOPMENT OF MEMBRANE CHIP SYSTEM FOR STRESS SENSOR

その他リンク： https://jlc.jst.go.jp/DN/JALC/00289812370?from=CiNii

Umakoshi Hiroshi, Shimanouchi Toshinori, Kuboi Ryoichi

Annual Report of FY 2006, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST) 21 - 24 2007年

3C10-4 Transglutaminase架橋Aβオリゴマーの疎水性とアミロイド線維形成過程に及ぼす効果(酵素学・酵素工学・タンパク質工学,その他,一般講演)

馬越大, 濱田聡, 島内寿徳, 久保井亮一

日本生物工学会大会講演要旨集 19 90 - 90 2007年

3C10-5 Roles of Liposomes and Heat Stress for Enhanced Release of Chitosanase from Streptomyces griseus :

NGO Kien Xuan, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

日本生物工学会大会講演要旨集 19 91 - 91 2007年

1B11-1 プロトフィブリルの表面特性に基づくアミロイド線維形成現象の解析(酵素学・酵素工学・タンパク質工学,一般講演)

島内寿徳, 廣岩梓, 西山圭一, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 19 59 - 59 2007年

DEVELOPMENT OF ELECTROACTIVE POLYMETHYLTHIOPHENE BASED DOPAMINE SENSOR

Vu Thi Huong, Kuboi Ryoichi, Pham Hung Viet, Shimanouchi Toshinori, Nguyen Minh Tue, Do Phuc Quan

Annual Report of FY 2006, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST) 41 - 46 2007年

DESIGN AND DEVELOPMENT OF OXIDATIVE STRESS RESPONSIVE LIPOSOME MEMBRANE WITH ENZYMATIC ACTIVITY (LIPOZYME) AND ITS APPLICATION TO CHEMICAL/BIOSENSOR

Kuboi Ryoichi, Shimanouchi Toshinori, Umakoshi Hiroshi, Le Quoc Tuan

Annual Report of FY 2006, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST) 63 - 66 2007年

LIPOzyme: design and development of artificial chaperone/enzyme liposome

2006年

Design of stress-mediated bioprocess using liposome - recovery of chitosanase from Streptomyces griseus cells

2006年

Study on Interaction of Amyloid b-Peptide with Liposome

2006年

Stress-mediated preparation of liposomes incorporating membrane protein

2006年

Membrane Chip: Dynamic Analysis of Stressed Membrane-Membrane Interaction

2006年

Membrane Stress Biotechnology

2006年

Use LIPOzyme for Stress-Mediated Bioreactor and Bioseparation

2006年

Design of Stress Sensor Using Conducting Polymers/Artificial Cell Membranes

7章2節 2006年

1F12-1 SOD Activity with Support of Phospholipid Membrane under Oxidative Stress :

TUAN Le quoc, NAGAMI Hideto, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

日本生物工学会大会講演要旨集 18 94 - 94 2006年

2F11-4 脂質膜界面を反応場とするアミロイドβ-金属錯体の酸化酵素様機能 : コレステロールの酸化反応(酵素学,酵素工学,タンパク質工学,一般講演)

田崎誠, 吉本則子, 島内寿徳, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 18 102 - 102 2006年

2F09-4 ドーパミン酸化誘導体によるアミロイド繊維の可溶化挙動の解析(酵素学,酵素工学,タンパク質工学,一般講演)

島内寿徳, 石川大介, 野澤篤司, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 18 99 - 99 2006年

2G09-4 複核金属型人工酵素リポソームのSOD-like活性の評価(酵素学,酵素工学,タンパク質工学/資源・エネルギー工学,一般講演)

小濱雄司, タンルクオク, 島内寿徳, 馬越大, 久保井亮一

日本生物工学会大会講演要旨集 18 120 - 120 2006年

Development of Chemical-and Bio-sensor for Environmental Monitoring

Shimanouchi Toshinori, Kuboi Ryoichi

Annual Report of FY 2005, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST) 41 - 46 2006年

Immobilized Liposome Chromatography

472-482 2005年

Metal-affinity immobilized liposome chromatography to detect conformational change of peptide

2005年

Heat enhanced recovery of chitosanase from Streptomyces griceus using aqueous two-phase system

2005年

Detection of Conformational Change of Protein Based on Protein-Liposome Interaction

2005年

Metal affinity-immobilized liposome chromatography for detection of conformational change of peptide

2005年

タンパク質の構造異常疾患におけるアミロイド線維の制御〜メンブレン・ストレスバイオテクノロジーの視点から〜招待

島内寿徳, 馬越大, 久保井亮一

分離技術 35 ( 6 ) 391 - 396 2005年

Preparation Method of Cell-Sized Liposomes

38-45 2005年

DESIGN AND DEVELOPMENT OF NANO-ARTIFICIAL-CELL MEMBRANE BASED NOVEL BIOSENSOR : APPLICATION FOR MONITORING OF AQUEOUS STRESSES

Kuboi Ryoichi, Morita Seiichi, Yoshimoto Makoto, Ho-Sup Jung, Shimanouchi Toshinori, Umakoshi Hiroshi

Annual Report of FY 2004, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST) 13 - 16 2005年

メンブレン・ストレス応答ダイナミクスのモニタリング技術の開発招待

島内寿徳, 森田誠一, 久保井亮一

生物工学会誌 82 ( 5 ) 208 - 211 2004年

Detection of Protein Conformation under Stress Condition Using Liposome as Sensor Materials

2004年

メンブレン・ストレスバイオテクノロジー--薬物送達システムの設計と開発への応用 (特集大きな可能性を秘めた研究開発)

久保井亮一, 馬越大, 島内寿徳

ケミカルエンジニヤリング 49 ( 4 ) 255 - 260 2004年

メンブレン・ストレスバイオテクノロジー--薬物送達システムの設計と開発への応用 (特集大きな可能性を秘めた研究開発)

久保井亮一, 馬越大, 島内寿徳

ケミカルエンジニヤリング 49 ( 4 ) 255 - 260 2004年

Metal Affinity-Immobilized Liposome Chromatography for Stress Mediated Separation of Biomolecules

Nagami Hideto, Umakoshi Hiroshi, Kitaura Takeaki, Thompson III Gary Lee, Shimanouchi Toshinori, Kuboi Ryoichi

アジア・太平洋化学工学会議発表論文要旨集 2004 991 - 991 2004年

記述言語：英語出版者・発行元：公益社団法人化学工学会

Investigation and utilization of peptides and proteins depend upon their separation and purification, which may be accomplished with chromatographic methods. Among the possible methods, immobilized metal affinity chromatography (IMAC), first reported by Porath, is known as a useful and powerful tool of the separation and analysis of the peptides and proteins and currently becomes the most popular chromatographic technique. A metal-affinity immobilized liposome chromatography (MA-ILC) was newly developed as a new chromatographic technique to separate and analyze the protein. The MA-ILC was prepared from the immobilized liposome modified with the functional ligands. A N-hexadodecyl -imonodiacetic acid (HIDA) was then used as the functional ligand. The metal ion Cu(II) was first adsorbed by the coordination with the functional ligand-modified liposome, immobilized on a gel matrix before the analysis of the elution behaviors. Synthetic peptides ranging in size from 5 to 40 residues were used to evaluate the retention properties of MA-ILC. The retention properties using usual imidazole elution for MA-ILC at 303 K was compared with those for IMAC, which is a most popular separation method for proteins, and an immobilized liposome chromatography (ILC), which can separate and analyze the protein mainly by the hydrophobic interaction between the liposome and protein. The retention ability of peptide on MA-ILC has both the properties of IMAC and ILC; the capacity factor depends on both the number of His residue in peptide and the hydrophobicity of peptide. It is considered that the retention properties of IMAC could be related not only to the hydrophobic interaction between the peptide and liposome but also to the cluster formation of the ligands on the liposome surface.

DOI： 10.11491/apcche.2004.0.991.0

Different Roles of Metal Ions Both in the Formation of Amyloid-β Fibri and Superoxide

Nagami Hideto, Kishida Manabu, Umakoshi Hiroshi, Shimanouchi Toshinori, Kuboi Ryoichi

アジア・太平洋化学工学会議発表論文要旨集 2004 498 - 498 2004年

記述言語：英語出版者・発行元：公益社団法人化学工学会

The roles of metal ions both on the formation of fibril aggregation of amyloid-β-peptide (Aβ) and on the generation of superoxide by a metal-Aβ complex have been studied to clarify the relationship of both phenomena with the cause of Alzheimer's Disease (AD). Among the several metal ions, the addition of Zn(II) or of Cu(II) was found to increase the formation of Aβ fibril. Although Zn(II) promoted the Aβ fibril formation both at neutral and acidic pH levels, Cu(II) could induce the aggregation of Aβ only at acidic pH. In addition, hydrogen peroxide was generated at neutral pH in the absence of Aβ and Cu(II) beyond a stoichiometric amount of Cu(II) ion, suggesting the formation of a catalytic site that can react between Cu(II) and Cu(I). These results indicate that Zn(II) and Cu(II) at different pHs may each contribute to AD in different modes; the promotion of fibril aggregation (Zn(II) at every pH and Cu(II) at acidic pH) and the formation of superoxide (Cu(II) at neutral pH). The most characteristic aspect is the effect of pH on the promotion of aggregation and the formation of superoxide for the Cu(II)-Aβ system. The coordination structure of the Cu(II)-Aβ complex at neutral and acidic pHs was analyzed by using NMR spectroscopy, circular dichroism (CD), and ESR. The formation of a complex of histidine (His) residues and Cu(II) was found, and the coordination number of the His was suggested to be two at neutral pH and three at acidic pH. These results imply that the intramolecular coordination structure of the Aβ -Cu(II) complex at neutral pH could be converted to the intermolecular one by acidification. The pH variation thus led to a change in the complex structure of Cu(II)-Aβ. This finding suggests the important roles of pH to regulate two phenomena, i.e. the aggregation of Aβ (inhibition of superoxide formation) and the formation of superoxide from Cu(II)-Aβ complex.

DOI： 10.11491/apcche.2004.0.498.0

Characterization of Stress-Response Behaviors of SOD/CAT on Liposome under Heat/Oxidative Stresses

Yoshimoto Noriko, Nagami Hideto, Umakoshi Hiroshi, Shimanouchi Toshinori, Kuboi Ryoichi

アジア・太平洋化学工学会議発表論文要旨集 2004 481 - 481 2004年

記述言語：英語出版者・発行元：公益社団法人化学工学会

A biological membrane is the frontier of the cells against the environmental reactive oxygen species (ROS) and is expected to play an important role in the antioxidant system corporately with antioxidant enzymes. The response behaviors of the antioxidant enzymes (superoxide dimutase (SOD) and catalase (CAT)) were investigated in the presence of artificial membrane, liposome, under the oxidative stress condition in order to obtain the fundamental information for the ROS scavenger system. The intracellular antioxidant enzymes, SOD and CAT, are known to make protective system against ROS. Although the SOD is known to catalyze the dismutation of ·O₂- to H₂O₂. The activity of SOD was lost thoroughly in the presence of H₂O₂ at high concentration (>5mM). The SDS-PAGE analysis showed that the SOD was fragmented into several small peptides. SOD has two -helix loops, which introduce the substrate ·O₂- to the activity center of SOD (Cu(II)). From the circular dichroism analysis of SOD in the presence of H₂O₂, the contents of α-helix in the SOD were found to decrease in corresponding with the inactivation of the SOD, suggesting that the conformation of the α-helix loops might be varied. However, the SOD was not inactivated in the presence of POPC liposomes while the contents of α-helix were decreased similarly in the case of that without liposome. Based on these results, the possibility to develop the ROS scavenger system using the SOD and CAT together with liposome was finally discussed.

DOI： 10.11491/apcche.2004.0.481.0

3I10-2 ストレス条件下におけるアミロイド形成挙動に及ぼす脂肪酸修飾リポソームの共存効果(生物化学工学・分離精製工学,一般講演)

李奉局, 西村大佑, 馬越大, 島内寿徳, 久保井亮一

日本生物工学会大会講演要旨集 16 229 - 229 2004年

3I10-1 リポソームによる新規なバイオセンサーの設計・開発 : 水相中ストレス成分のモニタリングへの応用(生物化学工学・分離精製工学,一般講演)

佐々木勝司, 李奉局, 島内寿徳, 森田誠一, 久保井亮一

日本生物工学会大会講演要旨集 16 229 - 229 2004年

固定化リポリームクロストグラフィーによる生理活性物質の分離・分析招待査読

ぶんせき 11 636 - 642 2003年

Characterization of the surface properties of chitosanase under heat stress condition using aqueous two-phase systems and an immobilized-liposome sensor system

HS Jung, H Umakoshi, SY Son, T Shimanouchi, R Kuboi

SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN 10 ( 10 ) 123 - 132 2003年

記述言語：英語出版者・発行元：JAPAN ASSOC SOLVENT EXTRACTION

The surface properties of chitosanase from Storeptomyces griseus were characterized under heat stress conditions by using aqueous two-phase systems and by using an immobilized-liposome sensor. The surface net (HFS) and local hydrophobicities (LH), were first characterized by using the poly (ethylene glycol) (PEG)/dextran 100-200k (Dex) aqueous two-phase system at various temperatures (25similar to50degreesC). The LH values of dithiothreitol (DTT)-treated chitosanase showed a maximum at a specific temperature (45degreesC) while the HFS values gradually increased with increasing temperature from 40 to 50degreesC. At the same temperature conditions as above, the surface properties of chitosanase were also analyzed by using a sensor tip immobilized-liposome electrode. Similarly in the case of the variation of LH but not HFS, the detected signal (maxima in electric current) was maximized at 45degreesC, and a linear correlation between the LH values and the signal values of the new immobilized-liposome sensor system was obtained. It was thus found that the new sensor system was effective for the characterization of the surface properties and LH values, of proteins, as well as the aqueous two-phase system method.

Web of Science