MISC - YOKOTA Kenji

PLATELET-AGGREGATION INDUCED BY UNCOMMON SEROTYPES OF STREPTOCOCCUS-SANGUIS ISOLATED FROM PATIENTS WITH BEHCETS-DISEASE

E ISOGAI, H ISOGAI, K YOKOTA, S HAYASHI, N FUJII, K OGUMA, K YOSHIKAWA, Y SASAMOTO, S KOTAKE, S OHNO

ARCHIVES OF ORAL BIOLOGY   36 ( 6 )   425 - 429   1991

　More details

Language：English   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Uncommon serotypes were tested for their ability to induce aggregation in platelet-rich plasma. Both uncommon and common serotypes induced platelet aggregation in rabbit platelet-rich plasma, but serotonin release was higher with the uncommon serotypes. Aggregation at ATP release varied between uncommon and common serotypes. With human platelet-rich plasma, only uncommon serotypes showed aggregation. Differences in serotype selectivity and the rate of aggregation were noted among platelet donors. About half of the patients with Behcet's disease and 30% of health controls showed platelet aggregation. Plasma from non-responder patients with Behcet's disease inhibited aggregation of healthy responder platelets within 20 min. Thus selective binding of uncommon serotypes of Strep. sanguis to plateletes might cause the vasculitis in Behcet's disease.

Web of Science

researchmap

Adhesive properties of streptococcus sanguis isolated from patients with behçet's disease

E. Isogap, H. Isogai, N. Fujii, K. Yokota, M. Yamaguchi, K. Oguma, K. Yoshikawa, Y. Sasamoto, S. Ohnot

Microbial Ecology in Health and Disease   3 ( 6 )   321 - 328   1990

　More details

Language：English   Publisher：Informa Healthcare  

Various strains of Streptococcus sanguis adhered to the buccal epithelial cells from both healthy controls and patients with Behçet's disease (BD). Almost all of them strongly adhered to the epithelial cells from patients with BD, whereas smaller numbers of the bacteria were observed on the cells from healthy controls. This phenomenon was especially clear when uncommon serotypes (clinical isolates) were used. Two or more peaks were observed in the distribution of the epithelial cells from patients with BD in the adherence test. In contrast, a single peak was observed in the distribution of the epithelial cells from healthy controls. Cytological differences of the epithelial cells between both groups were not observed in the microscopic image analysis. All the strains of S.sanguis showed agglutination of rabbit erythrocytes, but not agglutination of sheep, horse, swine and duck erythrocytes. Line cell adherence of S.sanguis uncommon serotypes was greater than that of common ones. All strains of S.sanguis showed a reactivity with aggregated human β2-microglobulin, but not with the monomer. The different level of reactivity to aggregated β2-microglobulin was recognised between uncommon and common serotypes. The reaction between aggregated β2-microglobulin and S.sanguis is one of human-bacterium interaction which should be considered in studies of BD. ©1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

DOI： 10.3109/08910609009140254

Scopus

researchmap