研究者詳細 - 横田　憲治

LIPID PROFILES OF HELICOBACTER-PYLORI AND HELICOBACTER-MUSTELAE GROWN IN SERUM-SUPPLEMENTED AND SERUM-FREE MEDIA 査読

M HAQUE, Y HIRAI, K YOKOTA, K OGUMA

ACTA MEDICA OKAYAMA 49 ( 4 ) 205 - 211 1995年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

Many of Helicobacter species have been found to have novel cholesteryl glucosides (CGs). To study the biosynthetic mechanism of CGs, the lipid profiles of H. pylori and H. mustelae grown in serum-supplemented and cholesterol-restricted serum-free media were investigated. In contrast to the serum-supplemented state, helicobacters had less CGs in the serum-free state; a trace amount of CGs and no CG was detected in H. pylori and H. mustelae, respectively. The proportion of total and individual phospholipid also showed significant alteration. Unknown lipids which did not contain phosphate and sugar were detected in the serum-free state, but not in the serum-supplemented state. The CGs were found to be distributed mainly in the membrane fractions, and one of the unknown lipids was found exclusively in the cytosol fraction. Based on these data,it is apparent that the CGs of helicobacters are synthesized by de novo uptake of cholesterol from the media. The unknown lipids detected in the serum-free state may be storage lipids, appearing in response to depletion of nutrients, especially cholesterol, or other factors in the media.

Web of Science

240 尋常性乾癬におけるCandida albicans血中抗体価の検討

田中智, 飯田憲治, 松田三千雄, 林俊治, 続佳代, 横田憲治, 小熊恵二

アレルギー 44 ( 3 ) 414 - 414 1995年

231 アトピー性皮膚炎におけるCandida albicans血中抗体価の検討

飯田憲治, 田中智, 林俊治, 続佳代, 松田三千雄, 横田憲治, 小熊恵二

アレルギー 44 ( 3 ) 412 - 412 1995年

Unique cholesteryl glucosides in Helicobacter pylori: Composition and structural analysis

Y. Hirai, M. Haque, T. Yoshida, K. Yokota, T. Yasuda, K. Oguma

Journal of Bacteriology 177 ( 18 ) 5327 - 5333 1995年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Society for Microbiology

A chloroform-methanol-extracted lipid of Helicobacter pylori was studied. Three kinds of glycolipids, accounting for about 25% (wt/wt) of the total lipid, were detected and identified to be cholesteryl glucosides. The structures of two of them were determined to be cholesteryl-α-D- glucopyranoside and cholesteryl-6-O-tetradecanoyl-α-D-glucopyranoside, and the plausible structure of the third one was identified as cholesteryl-6-O- phosphatidyl-α-D-glucopyranoside. Cholesteryl glucosides are very rare in animals and bacteria. Furthermore, those in H. pylori had an α-glycosidic linkage, which is rather unusual for natural glycosides, and a phosphate- linked cholesteryl glycoside like the cholesteryl-6-O-phosphatidyl-α-D- glucopyranoside has not been reported previously. As the cholesterol glucosides were detected in strains obtained from diverse geographical locations, the presence of cholesteryl glucosides in H. pylori is a very unique and a characteristic feature of the species. These findings add a new facet to the physiology and biochemistry, especially the cholesterol and glucose metabolism, of H. pylori. Furthermore, the cholesteryl glucosides of H. pylori showed hemolytic activities.

DOI： 10.1128/jb.177.18.5327-5333.1995

HELICOBACTER-PYLORI INDUCES INFLAMMATION IN MOUSE URINARY-BLADDER AND PELVIS 査読

H ISOGAI, E ISOGAI, K KIMURA, N FUJII, K YOKOTA, K OGUMA

MICROBIOLOGY AND IMMUNOLOGY 38 ( 5 ) 331 - 336 1994年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CENTER ACADEMIC PUBL JAPAN

Helicobacter pylori was transurethrally inoculated into the mouse urinary tract. The organism established infection and induced inflammation in the urinary bladder and pelvis. During the infection, urinary pH was elevated, probably due to the production of NH3 by bacterial urease. H. pylori was recovered from the urinary bladder, kidney and urine of the infected mice. Histopathologically, severe neutrophil infiltration was observed in the mucosal layer of both organs. H. pylori was detected on the surface of the epithelial cells. These results indicate that low pH and bacterial flora were not essential factors in establishing the mucosal infection with H. pylori. This experimental system is useful to investigate the pathogenicity of H. pylori in mucosal organs.

Web of Science

Heat Shock Protein Produced by Helicobacter pylori

Yokota Kenji, Hirai Yoshikazu, Haque Mahmudul, Hayashi Shyunji, Isogai Hiroshi, Sugiyama Toshiro, Nagamachi Eiko, Tsukada Yutaka, Fujii Nobuhiro, Oguma Keiji

MICROBIOLOGY and IMMUNOLOGY 38 ( 5 ) 403 - 405 1994年

記述言語：英語出版者・発行元：Center For Academic Publications Japan

The cells of Helicobacter pylori were suspended in the medium containing ³⁵S-methionine. After a heat shock of the cells at 42C for 5, 10, and 30min, the production of proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Out of many proteins produced by the cells, only 66kDa protein production was dramatically increased by heat treatment. The N-terminal amino acid sequence of 66kDa protein was quite similar to that of 62kDa and 54kDa proteins previously suggested as heat shock protein (HSP) of H. pylori based on the reaction with polyclonal and monoclonal antibodies against HSP 60 family proteins produced by other bacteria. Therefore, it was concluded that H. pylori produces the 66kDa protein as its major heat shock protein which belongs to HSP 60 family.

DOI： 10.1111/j.1348-0421.1994.tb01799.x

CiNii Article

356 アトピー性皮膚炎におけるCandida albicans血中抗体価の検討

飯田憲治, 田中智, 横田憲治, 小熊恵二, 松田三千雄

アレルギー 42 ( 9 ) 1398 - 1398 1993年

Lipid composition of Helicobacter pylori

Y. Hirai, M. Haque, K. Yokota, K. Oguma

Nippon rinsho. Japanese journal of clinical medicine 51 ( 12 ) 3094 - 3101 1993年

記述言語：日本語掲載種別：研究論文（学術雑誌）

In H. pylori, we examined the composition of total lipids. In thin-layer chromatography, three kinds of glycolipids were detected, which were named as G-1, G-2 and G-3. G-3 contained phosphates in addition to sugars. Total lipids consisted of 6.0% neutral lipids (weight %), 20.6% glycolipids (G-1, G-2) and 73.4% phospholipids (including G-3). Total glycolipids were approximately 25% of total lipids. After methanolysis, cholesterol was detected in the hexane extractable fraction and glucose was detected by GLC in the methanol fraction after trimethylsilylation, in all glycolipids. Therefore, these glycolipids are cholesteryl glycosides. Cholesteryl glycosides are found very rarely in bacteria, and have been detected only in a few species of mycoplasmas and spirochetes.

Experimental animal models in Mongolian gerbils and mice

H. Isogai, E. Isogai, K. Yokota, K. Oguma

Nippon rinsho. Japanese journal of clinical medicine 51 ( 12 ) 3138 - 3143 1993年

記述言語：日本語掲載種別：研究論文（学術雑誌）

Helicobacter pylori is an emerging candidate agent for the genesis of chronic gastritis and peptic ulcer. There have been many challenges to establish an experimental infection with H. pylori in animals. In recent, several studies reported for animal infection with this organism. One of them was the study using urinary system. The study indicated the ability of H. pylori to induce inflammation without significant low pH condition. Experimental infection in Mongolian gerbils showed that H. pylori could colonize on the surface of the gastric mucosa in animals without human. The study of nude mice by Karita et al. showed several valuable facts concerning the relation between bacterial pathogenicity and potential of bacterial colonization in stomach. The study in which remaining of bacteria was extended showed that colonization of H. pylori was affected by bacterial flora in the stomach.

Antibody Response to Oral Streptococci in Behçet's Disease

P 140 Characterization and functional properties of Streptococcus sanguis isolated from patient with Behçet's disease

E. Isogai, K. Yokota, N. Fujii, S. Hayashi, K. Kimura, H. Isogai, N. Ishii, Y. Yamakawa, H. Nakajima, S. Ohno, S. Kotake, K. Oguma

La Revue de medecine interne 14 ( 1 ) 123 1993年

YOKOTA Kenji, HAYASHI Syunji, FUJII Nobuhiro, YOSHIKAWA Kouji, KOTAKE Satoshi, ISOGAI Emiko, OHNO Shigeaki, ARAKI Yoshio, OGUMA Keiji

MICROBIOLOGY and IMMUNOLOGY 36 ( 8 ) 815 - 822 1992年

記述言語：英語出版者・発行元：Center For Academic Publications Japan

The serum antibody titers against oral streptococci were studied by enzyme-linked immunosorbent assay (ELISA) both in patients with Behçet's disease (BD) and control groups. The patients with BD showed significantly higher antibody titers to S. sanguis strains 113-20, 114-23, and 118-1 which were isolated from patients with BD, in comparison with control groups. Also, the reactions of high-titered sera to the crude cell wall and soluble (or membrane) fractions of the 113-20 strain were observed by western blot test. The sera of the patients with BD demonstrated strong bands of approximately 36kDa, 82kDa, and 87kDa in the crude cell wall fractions, and many bands of 80kDa to 150kDa in the membrane fractions, indicating that these proteins are the ones leading the high antibody titers to this bacterium in the sera of patients with BD.

DOI： 10.1111/j.1348-0421.1992.tb02083.x

CiNii Article

Colonization of Helicobacter pylori in the Gastric Mucosa of Mongolian Gerbils

Kenji Yokota, Keiji Oguma, Shunji Hayashi, Yoshikazu Takayama, Kouzou Imai, Tsuyoshi Yabana, Akira Yachi, Hiroshi Isogai, Emiko Isogai, Youichi Kurebayashi

Microbiology and Immunology 35 ( 6 ) 475 - 480 1991年

記述言語：英語掲載種別：研究論文（学術雑誌）

Helicobacter pylori was orally inoculated into Mongolian gerbils. The organisms were able to colonize in the gastro-mucosal layer of the gerbils, especially in those gerbils which had mucosal lesions caused by indomethacin treatment. The pathological changes developed by H. pylori infection were restricted to the stomachs, and only slightly inflammatory cells were observed. © 1991, Center For Academic Publications Japan. All rights reserved.

DOI： 10.1111/j.1348-0421.1991.tb01577.x

胃壁中のCampylobacter pyloriの同定法の検討と血中抗菌抗体の測定査読

横田憲治, 小熊恵二, 吉田博清, 高山義一, 杉山敏郎, 矢花剛, 谷内昭, 榑林陽一, 磯貝浩, 磯貝恵美子

感染症学会誌 64 597 - 603 1990年

Close association of Streptococcus sanguis uncommon serotypes with Behcet's disease 査読

Isogai, E, Ohno, S, Takeshi, K, Yoshikawa, K, Tsurumizu, T, Isogai, H, Yokota, K, Kotake, S, Sasamoto, Y, Hashimoto, T, Shimizu, H, Fujii, N, Yamaguchi, M, Oguma, K

Bifidobact. Microflora 9 27 - 41 1990年

Chemiluminescence of neutrophils from patients with Behçet's disease and its correlation with an increased proportion of uncommon serotypes of Streptococcus sanguis in the oral flora

E. Isogai, S. Ohno, S. Kotake, H. Isogai, T. Tsurumizu, N. Fujii, K. Yokota, B. Syuto, M. Yamaguchi, H. Matsuda, K. Oguma

Archives of Oral Biology 35 ( 1 ) 43 - 48 1990年

記述言語：英語掲載種別：研究論文（学術雑誌）

Zymosan-induced chemiluminescence was investigated in whole blood and in neutrophils: in both, the peak count was frequently elevated in Behçet's disease, and was significantly higher than in healthy controls
similarly the peak time was shorter. There were more uncommon serotypes of Streptococcus sanguis in the oral flora of patients with Behçet's disease. Common serotypes were present in the flora of healthy controls, but not in patients with the disease. The percentage of Strep. sanguis in the oral flora was significantly correlated with the level of chemiluminescence response. Thus infection with uncommon serotypes of Strep. sanguis may play a role in the aetiology of Behçet's disease. © 1990.

DOI： 10.1016/0003-9969(90)90113-O