研究者詳細 - 横田　憲治

論文 - 横田　憲治

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Endoscopic and Histological Gastritis in University Students with Helicobacter pylori Infection.

Shotaro Okanoue, Hiroyuki Sakae, Kenji Yokota, Takehiro Tanaka, Yuka Obayashi, Makoto Abe, Yoshiyasu Kono, Hiromitsu Kanzaki, Masaya Iwamuro, Seiji Kawano, Yoshiro Kawahara, Hiroyuki Yanai, Hiroyuki Okada

Internal medicine (Tokyo, Japan) 63 ( 21 ) 2875 - 2884 2024年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Objective Although the characteristics of Helicobacter pylori infection have been extensively reported, there is a lack of consensus regarding its characteristics in young adults. The present study examined the endoscopic and histological characteristics of young adults who underwent eradication therapy for H. pylori infection. Methods We examined the H. pylori infection status of first-year students at Okayama University School of Medicine and Dentistry between 2014 and 2020. A total of 152 (6.8%) students who were positive for H. pylori antibody or pepsinogen tests were enrolled in the study. Among them, 107 students underwent endoscopy, and their biopsy samples were investigated. Seventy-five students were diagnosed with H. pylori infections. Results Of 75 H. pylori-positive patients, 57 (76.0%) had endoscopic atrophic gastritis, and 42 (56.0%) had histological atrophy. A few patients had severe atrophic gastritis. All 65 patients who underwent an eradication assessment were successfully treated. After successful eradication, 26 patients underwent endoscopic follow-up. The mean follow-up period was 32.9 months. A histological evaluation revealed that gastric antrum atrophy had subsided in 11 of 14 patients, and atrophy in the lesser curvature of the gastric body had subsided in 7 of 8 patients. Conclusion More than half of young adults with H. pylori infection had atrophic gastritis. We found mild atrophy in young adults, which subsided shortly after eradication treatment. This study provides a foundation for future studies to evaluate the validity of eradication therapy in preventing gastric cancer in patients.

DOI： 10.2169/internalmedicine.1851-23

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胃炎-胃癌患者の胃粘膜より分離された硝酸塩還元菌の性状

桑木星里香, 山本由弥子, 内山淳平, 松下治, 後藤和義, 渡辺朱理, 横田憲治

日本細菌学雑誌 79 ( 2 ) 152 - 152 2024年6月

基礎研究1 ピロリ菌感染モデルにおける細胞性免疫の低下と硝酸塩還元菌の影響

横田憲治, 内山淳平, 倉岡紗樹子, 岡上昇太郎, 河野吉泰, 岡田裕之

日本ヘリコバクター学会学術集会プログラム・抄録集 30回 70 - 70 2024年6月

胃癌患者の胃内より分離した硝酸塩還元菌のピロリ菌共感染マウスへの影響

小松原万里奈, 山本由弥子, 内山淳平, 松下治, 後藤和義, 渡辺朱理, 横田憲治

日本細菌学雑誌 79 ( 2 ) 140 - 140 2024年6月

消化管細菌叢と胃の疾患胃癌患者の胃内より分離される硝酸塩還元菌ついて

横田憲治, 倉岡紗樹子, 岡上昇太郎, 河野吉泰, 岡田裕之

日本ヘリコバクター学会学術集会プログラム・抄録集 29回 95 - 95 2023年6月

Helicobacter pylori感染胃炎における硝酸塩還元菌の胃内生息状況に関する検討

倉岡紗樹子, 横田憲治, 榮浩行, 小嶋日菜子, 岡上昇太郎, 河野吉泰, 岡田裕之

日本ヘリコバクター学会誌 24 ( 2 ) 110 - 116 2023年1月

Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells. 国際誌

Hinako Ojima, Sakiko Kuraoka, Shyoutarou Okanoue, Hiroyuki Okada, Kazuyoshi Gotoh, Osamu Matsushita, Akari Watanabe, Kenji Yokota

Microorganisms 10 ( 12 ) 2022年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development.

DOI： 10.3390/microorganisms10122495

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Helicobacter pylori感染胃炎における硝酸塩還元菌の胃内生息状況に関する検討

倉岡紗樹子, 横田憲治, 榮浩行, 小嶋日菜子, 岡上昇太郎, 河野吉泰, 岡田裕之

日本ヘリコバクター学会学術集会プログラム・抄録集 28回 106 - 106 2022年6月

若年者におけるヘリコバクターピロリ除菌治療による腸内細菌叢の変化についての検討

岡上昇太郎, 後藤和義, 倉岡紗樹子, 稲生祥子, 里見拓也, 濱田健太, 河野吉泰, 神崎洋光, 岩室雅也, 川野誠司, 河原祥朗, 横田憲治, 岡田裕之

日本ヘリコバクター学会学術集会プログラム・抄録集 28回 103 - 103 2022年6月

ヘリコバクター感染の基礎研究の現況 Helicobacter pyloriと共培養される胃内硝酸塩還元菌の細胞に対する作用

小嶋日菜子, 倉岡沙樹子, 岡上昇太郎, 河野吉泰, 岡田裕之, 横田憲治

日本ヘリコバクター学会学術集会プログラム・抄録集 28回 85 - 85 2022年6月

Establishment of a reference panel of Helicobacter pylori strains for antimicrobial susceptibility testing. 国際誌

Kenji Yokota, Takako Osaki, Shunji Hayashi, Shin-Ichi Yokota, Hiroaki Takeuchi, Emiko Rimbara, Hinako Ojima, Toyotaka Sato, Hideo Yonezawa, Keigo Shibayama, Kengo Tokunaga, Shigeru Kamiya, Kazunari Murakami, Mototsugu Kato, Toshiro Sugiyama

Helicobacter 27 ( 3 ) e12874 2022年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Eradication treatment for Helicobacter pylori gastritis is covered by national health insurance since 2013 in Japan. However, eradication failure due to the increase of antimicrobial resistance has become a serious problem. The present study aims to establish a reference panel of Japanese H. pylori strains for antimicrobial susceptibility testing. METHOD: A total of 28 strains were collected from 4 medical facilities in Japan. Antimicrobial susceptibility tests (ASTs) to clarithromycin (CLR), amoxicillin (AMX), and metronidazole (MNZ), were used to select standard reference strains. Complete genome sequences were also determined. RESULTS: Three H. pylori strains (JSHR3, JSHR6 and JSHR31) were selected as standard reference strains by the Japanese Society for Helicobacter Research (JSHR). The minimum inhibitory concentrations (MICs) of the antibiotics against these 3 strains by agar dilution method with Brucella-based horse-serum-containing agar medium were as follows: JSHR3 (CLR 16 μg/ml, AMX 0.032 μg/ml and MNZ 4 μg/ml), JSHR6 (CLR 0.016 μg/ml, AMX 0.032 μg/ml and MNZ 4 μg/ml), and JSHR31 (CLR 16 μg/ml, AMX 1 μg/ml and MNZ 64 μg/ml). CONCLUSIONS: A reference panel of H. pylori JSHR strains was established. The panel consisted of JSHR6, which was antibiotic-susceptible, JSHR3, which was CLR-resistant, and JSHR31, which was multi-resistant. This reference panel will be essential for standardized ASTs before the optimal drugs are selected for eradication treatment.

DOI： 10.1111/hel.12874

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Use of ATP Bioluminescence Assay to Evaluate Oral Streptococci.

Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Susumu Kokeguchi, Hiro-O Ito, Miwa Matsuyama

Biocontrol science 27 ( 4 ) 229 - 233 2022年

記述言語：英語掲載種別：研究論文（学術雑誌）

The statistical correlation between the number of oral streptococci and the results of ATP bioluminescence assay was examined and compared with the results from Streptococcus plate counts and an oral bacteria quantification system. Because a significant correlation was found between ATP (RLU) and the number of bacteria in the oral bacteria quantification system for all seven types of oral streptococci examined, ATP would reflect a conditions of oral hygiene. However, using this assay, it was observed it may be difficult to correctly evaluate bacteria that form aggregates. Furthermore, even a small number of bacteria (below 105 CFU/mL) , which cannot be measured by the oral bacteria quantification system, could be estimated by using ATP bioluminescence assay. It was suggested that this assay could be used for quantitative evaluation of the effect of oral cleaning.

DOI： 10.4265/bio.27.229

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Helicobacter pylori感染胃炎における硝酸塩還元菌の胃内生息状況に関する検討

倉岡紗樹子, 横田憲治, 榮浩行, 岡上昇太郎, 河野吉泰, 岡田裕之

日本ヘリコバクター学会学術集会プログラム・抄録集 27回 196 - 196 2021年9月

大学新入生ピロリ検診の検討

岡上昇太郎, 岡田裕之, 田中健大, 横田憲治, 倉岡紗樹子

日本ヘリコバクター学会学術集会プログラム・抄録集 27回 201 - 201 2021年9月

Elizabethkingia anophelis, an emerging pathogen, inhibits RAW 264.7 macrophage function. 国際誌

I Putu Bayu Mayura, Kazuyoshi Gotoh, Hayato Nishimura, Erina Nakai, Takehiko Mima, Yumiko Yamamoto, Kenji Yokota, Osamu Matsushita

Microbiology and immunology 65 ( 8 ) 317 - 324 2021年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Elizabethkingia anophelis is a pathogen that can cause a life-threatening infection in immunocompromised patients. The first case of E. anophelis infection was reported in 2013; subsequently, an increase in its incidence has been reported globally. Additionally, a mortality rate of more than 30% was observed in the US outbreak of 2015. To date, the pathogenic mechanisms underlying E. anophelis infection, such as toxin production, remain unclear. Since tissue macrophages act as the first line of defense against pathogens, in the present study the interactions between E. anophelis and a macrophage-like cell line RAW 264.7 were examined. Although E. anophelis showed no cytotoxicity toward RAW 264.7 macrophages, the infection inhibited LPS-induced morphological changes and activation of differentiation markers for the polarization of RAW 264.7 macrophages toward an M1-like phenotype. However, when the cell contact was restricted using Transwell inserts or bacterial culture supernatants were used instead of live bacteria, no such inhibition was observed. Moreover, it was shown that E. anophelis evaded phagocytosis. Overall, the results suggest that E. anophelis infection inhibits the differentiation of RAW 264.7 macrophages to a pro-inflammatory phenotype in a contact-dependent manner.

DOI： 10.1111/1348-0421.12888

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Serodiagnosis and bacterial genome of helicobacter pylori infection

Aina Ichihara, Hinako Ojima, Kazuyoshi Gotoh, Osamu Matsushita, Susumu Take, Hiroyuki Okada, Akari Watanabe, Kenji Yokota

Toxins 13 ( 7 ) 2021年7月

掲載種別：研究論文（学術雑誌）

The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori.

DOI： 10.3390/toxins13070467

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カルバペネマーゼ産生腸内細菌科細菌に対するビアペネムの殺菌効果

三好諒, I Putu Bayu Mayura, 後藤和義, 美間健彦, 山本由弥子, 横田憲治, 松下治, 萩谷英大

日本細菌学雑誌 76 ( 1 ) 119 - 119 2021年2月

敗血症性肺塞栓症患者から分離されたTsukamurella inchonensisの同定(Identification of Tsukamurella inchonensis isolated from septic pulmonary emboli(SPE) patient)

I Putu Bayu Mayura, Gotoh Kazuyoshi, 美間健彦, 山本由弥子, 横田憲治, 松下治, 萩谷英大

日本細菌学雑誌 76 ( 1 ) 89 - 89 2021年2月

Environmental survey of Methicillin-Resistant Staphylococci in a Hospital in Japan.

Akari Watanabe, Tokiko Watanabe, Susumu Kokeguchi, Yumiko Yamamoto, Osamu Matsushita, Kenji Yokota

Biocontrol science 26 ( 3 ) 137 - 145 2021年

記述言語：英語掲載種別：研究論文（学術雑誌）

We examined the hospital-wide incidence of methicillin-resistant Staphylococcus contamination in a hospital environment to predict the risk of the nosocomial spread of infection. Samples were also taken different surfaces and medical equipment in a general hospital ward and a staff station. The isolates were identified bacterial strains and analyzed by PCR for detection of the mecA gene and staphylococcal cassette chromosome mec (SCCmec) types (I-V). Overall, out of 146 isolates that were screened, 15.7% of the samples in the hospital wards were contaminated with Staphylococcus aureus and 74.7% were isolated with coagulase-negative Staphylococci (CNS). The methicillin-resistant mecA gene was detected in all oxacillin-resistant S. aureus, and 89% of oxacillin-resistant CNS was identified as methicillin-resistant S. aureus (MRSA) and MRCNS respectively. All S. aureus and CNS from the hospital wards with MRSA patients were detected as MRSA and MRCNS. A widespread distribution of MRSA and MRCNS was detected in the Cuff. The majority of the MRSA and MRCNS isolates in this study were SCCmec type V, which are a community-acquired infection type. The increased incidence and prevalence of community-acquired MRSA and MRCNS, as well as hospital-acquired MRSA, should be recognized as serious healthcare problems.

DOI： 10.4265/bio.26.137

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Antibacterial effects of disulfiram in helicobacter pylori

Tomomi Kobatake, Keiki Ogino, Hiroyuki Sakae, Kazuyoshi Gotoh, Akari Watanabe, Osamu Matsushita, Hiroyuki Okada, Kenji Yokota

Infection and Drug Resistance 14 1757 - 1764 2021年

掲載種別：研究論文（学術雑誌）

Background: Helicobacter pylori infection poses a risk of the occurrence of gastrointestinal diseases, such as gastric cancer. Its incidence rate is significantly reduced by eradication, and thereby, eradication therapy is generally performed. Disulfiram is an oral prescription drug mainly used for the treatment of alcohol dependence. In recent years, reports have been made on its anticancer and antibacterial effects, and thus, it has recently become an interesting subject. This study aimed to examine the antibacterial activity of disulfiram, investigate the presence or absence of its antibacterial activity on H. pylori, and determine whether it could be a new bactericidal drug against drug-resistant H. pylori. Materials and Methods: Drug-sensitive strains of H. pylori and amoxicillin-resistant, clarithromycin-resistant, and metronidazole-resistant strains were used, and a growth inhibition test of H. pylori using disulfiram was performed. Furthermore, the expression of urease, vacuolating cytotoxin A (VacA), and CagA, the virulence proteins of H. pylori, was quantitatively analyzed using the Western blotting method. In addition, for H. pylori used in this study, the 16SrDNA sequence, a ribosomal gene involved in protein production, was analyzed to examine the presence or absence of gene mutation. Results: Disulfiram suppressed the growth of 7 out of 12 H. pylori strains at 1 µg/mL, and no correlation was observed between their susceptibility/resistance to current eradication antimicrobial drugs and disulfiram resistance. Disulfiram reduced the expression levels of urease, VacA, and CagA proteins. H. pylori, which showed resistance to disulfiram, tended to have fewer gene deletions/insertions in the 16S rDNA sequence; however, no specific mutation was detected. Conclusion: Disulfiram has a bactericidal effect on H. pylori at low concentrations, suggesting that it can be used as a supplement for current H. pylori eradication drugs.

DOI： 10.2147/IDR.S299177

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