研究者詳細 - 窪木　拓男

Intradermal injection of Botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay 査読

Influence of resin coating materials on Porphyromonas gingivalis attachment. 査読

Ai Kumada, Yoshizo Matsuka, Atsushi Mine, Mitsuaki Ono, Junji Uehara, Norihiro Sonoi, Takashi Ito, Shogo Takashiba, Takuo Kuboki

Dental materials journal 31 ( 1 ) 86 - 91 2012年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：1

Resin coating materials have been used for composite resin or provisional restoration in order to prevent plaque accumulation on their surfaces. However, it is not clear whether the coating materials influence attachment of periodontal bacteria. Therefore, we investigated the effect of resin coating materials on the attachment of Porphyromonas gingivalis (Pg). The polymerized auto cure resin plates were coated with two resin coating materials. To estimate the Pg attachment, each plate was immersed in brain heart infusion medium containing Pg. The quantity of bacteria attached on each plate was evaluated by crystal violet quantification. Morphological change of Pg was recorded using scanning electron microscopy. Both coating groups presented significantly lower Pg attachment compared to the control. The Pg shapes on the plates with resin coating materials were similar to the non-treated control plates. The resin coating materials clearly prevent Pg attachment on the polymerized auto cure resin plate.

DOI： 10.4012/dmj.2011-164

Effect of low-shrinking composite on the bonding effectiveness of two adhesives in occlusal Class-I cavities.

Atsushi Mine, Jan De Munck, Marcio V Cardoso, Kirsten L Van Landuyt, André Poitevin, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Bart Van Meerbeek

Dental materials journal 31 ( 3 ) 418 - 26 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

The purpose of this study was to evaluate if a low-shrinking composite can improve the bonding effectiveness of adhesives in highly constrained conditions. A low-shrinking composite ('els-extra low shrinkage', Saremco) was bonded in standardized occlusal Class-I cavities using a three-step ('cmf', Saremco) and a two-step etch-and-rinse adhesive ('XP Bond', Dentsply). Both adhesives were also combined with a conventional composite ('Z100', 3M ESPE). Half of the restored cavities were exposed to 20,000 thermo-cycles. 3-way ANOVA revealed a significant effect for the factors 'adhesive' and 'composite' (both p<0.0001), but not for 'thermo-cycling' (p=0.994). Significantly higher bond strengths were recorded for the low-shrinking composite than for the control composite, using either of the adhesives. The low-shrinking composite in combination with the three-step etch-and-rinse adhesive performed best in the high C-factor Class-I cavity. The two-step etch-and-rinse adhesive suffered strongly from polymerization-shrinkage stress, which could be partially restored by using the low-shrinking composite.

DOI： 10.4012/dmj.2011-261

Kumada A, Matsuka Y, Spigelman I, Maruhama K, Yamamoto Y, Neubert J.K, Nolan T.A, Watanabe K, Maekawa K, Kamioka H, Yamashiro T, Kuboki T, Oguma K

Journal of Oral Rehabilitation 39 ( 1 ) 63 - 72 2012年

Application of purified botulinum type a neurotoxin to treat experimental trigeminal neuropathy in rats and patients with urinary incontinence and prostatic hyperplasia. 査読国際誌

Yoshizo Matsuka, Teruhiko Yokoyama, Yumiko Yamamoto, Tomonori Suzuki, Ni Nengah Dwi Fatmawati, Atsushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Takuo Kuboki, Atsushi Nagai, Keiji Oguma

Journal of toxicology 2012 648384 - 648384 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）

Type A neurotoxin (NTX) of Clostridium botulinum was purified by a simple procedure using a lactose gel column. The toxicity of this purified toxin preparation was retained for at least 1 year at -30°C by supplementation with either 0.1% albumin or 0.05% albumin plus 1% trehalose. When purified NTX was used to treat 49 patients with urinary incontinence caused by either refractory idiopathic or neurogenic detrusor overactivity, 36 patients showed significant improvement in symptoms. These beneficial effects were also observed in cases of prostatic hyperplasia. The results obtained with NTX were similar to that of Botox. The effects of NTX on trigeminal neuralgia induced by infraorbital nerve constriction (IoNC) in rats were also studied. Trigeminal ganglion neurons from ipsilateral to IoNC exhibited significantly faster onset of FM4-64 release than sham-operated contralateral neurons. Intradermal injection of NTX in the area of IoNC alleviated IoNC-induced pain behavior and reduced the exaggerated FM4-64 release in trigeminal ganglion neurons.

DOI： 10.1155/2012/648384

Occlusal dysesthesia: a qualitative systematic review of the epidemiology, aetiology and management 査読

Hara, E. S., Matsuka, Y., Minakuchi, H., Clark, G. T., Kuboki, T.

Journal of Oral Rehabilitation 39 ( 8 ) 630 - 638 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）

Summary Occlusal dysesthesia refers to a persistent complaint of uncomfortable bite sensation with no obvious occlusal discrepancy. This systematic review aimed to draw a picture of such patients, to present an agreement of previously reported diagnostic criteria and to analyse the evidence level of the recommended management approaches. An electronic search for all relevant reports on occlusal dysesthesia was thoroughly performed based on previous nomenclatures (e.g. phantom bite, occlusal hyperawareness) in PubMed and The Cochrane Library in July, 2011. A total of 84 reports were matched, among which only 11 studies were included after a two-step (abstract and detailed full-text revision) screening process. Additionally, a thorough manual review of reference lists of the included reports enabled the inclusion of two additional studies. Data analysis revealed that 37 occlusal dysesthesia patients presented a mean age of 51・7 ± 10・6 years and were predominantly women (male/female: 1/5・1) with symptom duration of more than 6 years (average: 6・3 ± 7・5 years) and with concomitant psychological disturbances (e.g. mood disorders, somatoform disorders, personality disorders). Only four authors presented diagnostic criteria for occlusal dysesthesia, which served as the basis for an agreement in the diagnostic criteria. Treatment approaches included psychotherapy, cognitive/behaviour therapy, splint therapy and prescription of anti-depressants or anti-anxiety drugs. Classification of evidence level of management approaches, however, revealed that most of them were expert opinions with single- or multiple-case report(s). Future studies are necessary for a deeper understanding of the mechanisms behind the occlusal dysesthesia symptoms, and consequently, for improvements in evidence-based management approaches.

DOI： 10.1111/j.1365-2842.2012.02300.x

Response shift in oral health-related quality of life measurement in patients with partial edentulism 査読

Kimura, A., Arakawa, H., Noda, K., Yamazaki, S., Hara, E. S., Mino, T., Matsuka, Y., Mulligan, R., Kuboki, T.

Journal of Oral Rehabilitation 39 ( 1 ) 44 - 54 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）

The purposes of this study were to determine whether a response shift was observable after partial denture treatment and to identify the predictors that influenced the response shift magnitude and direction. A total of 173 consecutive patients with no more than eight missing teeth who received implant-supported, fixed or removable partial dentures at Okayama University Dental Hospital were asked to complete a full-version Oral Health-Related Quality of Life (OHRQoL) questionnaire before (pre-test) and after treatment (post-test). Additionally, a short form (then-test) consisting of seven questions selected from the full version had its reliability verified and was utilised to retrospectively assess the pre-treatment OHRQoL status. The difference between the summary scores of the then-test and the pre-test determined the response shift magnitude and direction. The then-test mean score (22・9 ± 6・6) was significantly lower (worse OHRQoL) than that of the pre-test (26・4 ± 5・2). The response shift effect size was of moderate magnitude and negative direction (d = -0・78). A multiple regression analysis showed that age (younger patients) (P < 0・01), number of replaced teeth (fewer) (P < 0・01) and pre-test scores (lower) (P < 0・01) were the significant predictors for response shift. In conclusion, a response shift phenomenon with negative and moderate effect size was observed after partial denture treatment. The significant predictor variables were young age, fewer numbers of replaced teeth and lower pre-test scores.

DOI： 10.1111/j.1365-2842.2011.02241.x

顎関節症の診療ガイドラインに関するプロジェクト研究—スプリント治療ガイドラインの確立—．査読

The association between oral health-related quality of life and self-rated presenteeism 査読

Takiuchi Hiroya, Kimura-Ono Aya, Arakawa Hikaru, Mino Takuya, Masaki Asuka, Hara Emilio Satoshi, Kanyama Manabu, Matsuka Yoshizo, Kuboki Takuo

Quality of Life Research 21 71 - 72 2012年

A problem-based learning tutorial on elder inpatients in a nursing home in Japan. 査読

Matsuka Y, Nakajima R, Miki H, Kimura A, Kanyama M, Minakuchi H, Shinkawa S, Takiuchi H, Nawachi K, Maekawa K, Arakawa H, Fujisawa T, Sonoyama W, Mine A, Hara ES, Kikutani T, Kuboki T

Journal of Dental Education 2012年

軟骨細胞に対する、CCN2モジュールの単独・結合下での影響に関する評価(Evaluation of independent and combinational effect of CCN2 modules on chondorocytic cells)

El Kdaer Tarek Abd, Kubota Satoshi, Nishida Takashi, Hattori Takako, Aoyama Eriko, Janune Danilo, Kuboki Takuo, Takigawa Masaharu

日本骨代謝学会学術集会プログラム抄録集 29回 248 - 248 2011年7月

CCN2/CTGF誘導因子であるハルミンは軟骨形成を促進し、TNF-α誘発炎症反応を抑制する(HARMINE, AN INDUCER OF CCN2/CTGF, PROMOTES CHONDROGENESIS AND SUPPRESSES TNF-α-INDUCED INFLAMMATORY RESPONSE)

Hara Emilio S.i, Ono Mitsuaki, Kubota Satoshi, Sonoyama Wataru, Hattori Takako, Takigawa Masaharu, Kuboki Takuo

日本骨代謝学会学術集会プログラム抄録集 29回 232 - 232 2011年7月

RELATIONSHIP BETWEEN SLEEP BRUXISM FREQUENCY AND UPTAKE ABILITY OF HUMAN PERIPHERAL SEROTONIN TRANSPORTER 査読

Minakuchi H, Hara E. S, Sogawa C, Miki H, Fujisawa T, Maekawa K, Matsuka Y, Sogawa N, Kitayama S, Kuboki T

Sleep 34 A201 - A202 2011年

Peripheral administration of botulinum toxin type A neurotoxin reverses trigeminal neuropathic pain symptoms 査読

Ai Kumada, Yoshizo Matsuka, Igor Spigelman, John K Neubert, Todd A Nolan, Yumiko Yamamoto, Keiko Sato, Kazuhiro Watanabe, Takuo Kuboki, Keiji Oguma

http://f1000.com/posters/browse/summary/763 2011年

The relationship between temporomandibular joint pathosis and muscle tenderness in the orofacial and neck/shoulder region. 査読

Eitoku Inoue, Kenji Maekawa, Hajime Minakuchi, Chiyomi Nagamatsu-Sakaguchi, Tsuyoshi Ono, Yoshizo Matsuka, GT Clark, Takuo Kuboki

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics Vol.109 ( No.1 ) 86 - 90 2010年1月

周術期管理センター(歯科部門)のニーズと現況について経口気管挿管時に起こる歯の損傷とその対応

縄稚久美子, 山中玲子, 曽我賢彦, 柳文修, 兒玉直紀, 中田貴, 森田学, 窪木拓男, 足羽孝子, 森田潔

日本集中治療医学会雑誌 17 ( Suppl. ) 374 - 374 2010年1月

皆木省吾, 築山能大, 有馬太郎, 市川哲雄, 窪木拓男, 兒玉直紀, 佐久間重光, 新谷明喜, 高津匡樹, 竹内哲男, 津賀一弘, 坪井明人, 中島啓一朗, 中野雅徳, 成田紀之, 波多野泰夫, 藤澤政紀, 船登雅彦, 鱒見進一, 松香芳三

日歯会誌 29 62 - 66 2010年

記述言語：日本語出版者・発行元：日本歯科医学会

顎関節症の治療に関しては現時点では充分なエビデンスが集積しているとは言い難く、診療に必要とされるガイドラインの作成や関連するエビデンス蓄積の必要性が高まっている。本プロジェクト研究は、1)現在顎関節症の治療に用いられているスタビライゼーション・スプリントのデザインならびに臨床介入術式についてのガイドラインを作成すること、2)補綴系顎関節症専門治療施設ならびに開業歯科医院を対象としてその治療実態に関する調査を行うこと、3)顎関節雑音に対するスタビライゼーション・スプリントの効果に関する文献的エビデンスを収集すること、の3点を目的とした。結果として、スタビライゼーション・スプリントのデザインならびに臨床介入術式についてはDelphi法変法を用いた合意形成を行い、介入手法ガイドライン(案)をまとめた。また、治療実態調査については、顎関節症外来14施設および開業歯科医院33施設を対象とした調査を行い、診療時間等に関する結果を得ることができた。また、スタビライゼーション・スプリントを用いた治療に関するエビデンス検索では、顎関節雑音の治療に有効であるとするエビデンスは現時点では得ることができなかった。(著者抄録)

Novel Transcriptional Regulation of CCN2/CTGF by Nuclear Translocation of MMP3 査読

Peripheral administration of botulinum toxin type A decreases exaggerated neurotransmitter release and reverses neuropathy-induced gene expression changes in trigeminal ganglia 査読

Ai Kumada, Yoshizo Matsuka, Igor Spigelman, Yoshihito Ishihara, Yumiko Yamamoto, Takayuki Hikasa, Wataru Sonoyama, Hiroshi kamioka, Takashi Yamashiro, Takuo Kuboki, Keiji Oguma

http://f1000.com/posters/browse/summary/87 2010年

Takanori Eguchi, Satoshi Kubota, Kazumi Kawata, Yoshiki Mukudai, Junji Uehara, Toshihiro Ohgawara, Soichiro Ibaragi, Akira Sasaki, Takuo Kuboki, Masaharu Takigawa

CCN PROTEINS IN HEALTH AND DISEASE: AN OVERVIEW OF THE FIFTH INTERNATIONAL WORKSHOP ON THE CCN FAMILY OF GENES 255 - + 2010年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：SPRINGER-VERLAG BERLIN

CCN2/CTGF, previously known as Connective Tissue Growth Factor, is a crucial regulator of extra-cellular matrix (ECM), which promotes ECM synthesis and stabilization. As their family name clearly implies, matrix metalloproteases (MMPs) are also localized in the ECM, where they function as proteases, modulating cell signaling by cleaving proteins such as matrix proteins, growth factors and growth factor receptors. Strong expression of CCN2/CTGF in chondrocytic cells occurs through transcription enhancer dominant in chondrocytes (TRENDIC). Matrix metalloprotease-3 (MMP3) is a novel TRENDIC-binding transcription factor for CCN2/CTGF expression. First, MMP3 cDNA was cloned as a TRENDIC-binding factor by Southwestern screening. The interaction between MMP3 and TRENDIC was confirmed by a gel shift assay and chromatin immunoprecipitation. The CCN2/CTGF promoter was activated by transfected MMP3, whereas a TRENDIC mutant for the promoter lost the response. In addition, the knockdown of MMP3 suppressed CCN2/CTGF expression. Cytochemical and histochemical analyses demonstrated that MMP3 was detected in the nuclei of chondrocytic cells in culture and also in the nuclei of normal and osteoarthritic chondrocytes in vivo. The nuclear translocation of externally added recombinant MMP3 was observed in 30 min after the addition, and six putative nuclear localization signals were found in MMP3. These results indicated a novel trans-activation mechanism of CCN2/CTGF by the nuclear translocation of MMP3 through binding with TRENDIC in chondrocytes. Although MMPs historically had been recognized as a protease for extra-cellular proteins, this study indicated that it also stimulates ECM synthesis through CCN2/CTGF trans-activation. This novel regulatory role of the ECM may contribute to understanding the mechanism of not only the development, but also the pathogenesis of arthritis fibrosis and periodontitis.

DOI： 10.1007/978-90-481-3779-4_19

Scopus

顎関節症のスプリント治療に関する実態調査－大学病院顎関節症外来および一般開業歯科医院における診療時間の比較－査読

その他リンク： http://orcid.org/0000-0002-9600-4430

兒玉直紀, 築山能大, 有馬太郎, 市川哲雄, 窪木拓男, 佐久間重光, 新谷明喜, 高津匡樹, 津賀一弘, 坪井明人, 中野雅徳, 成田紀之, 波多野泰夫, 藤澤政紀, 船登雅彦, 鱒見進一, 松香芳三, 皆木省吾

日本顎関節学会雑誌 21 ( 3 ) 222 - 227 2009年12月

記述言語：日本語出版者・発行元：(一社)日本顎関節学会

目的:顎関節症患者に対する受診ごとの診療時間に関する報告はない。今回、大学病院顎関節症外来ならびに一般開業歯科医院を対象に顎関節症のスプリント治療に要する時間について調査を行い、その特性を明らかにすることを目的とした。方法:大学病院顎関節症外来14施設および一般開業歯科医院33施設にて2ヵ月間の調査を行った。スプリント治療を選択された顎関節症患者を対象に、スプリントの種類、各診療内容および要した時間を調査項目として施設間で比較検討した。結果:1回当たりの診療時間に関して、大学病院顎関節症外来受診患者(以下、大学群と略す)のほうが一般開業歯科医院受診患者(以下、開業医群と略す)に比べて有意に長かった。また、初診時の診療時間についても大学群のほうが開業医群に比べて長い時間を要した。しかし、スプリント装着および調整に要する時間について有意差は認められなかった。結論:顎関節症患者の1回当たりの診療に要する時間は両施設ともに比較的長時間であることがわかった。スプリント装着に30分以上要する割合は大学群においては44%であり、開業医群においては22%であった。スプリント調整に20分以上要する割合は、大学群においては48%であり、開業医群においては33%であった。(著者抄録)

DOI： 10.11246/gakukansetsu.21.222

J-GLOBAL