2021/11/27 更新

写真a

ツルタ タケシ
鶴田 剛司
TSURUTA Takeshi
所属
環境生命科学学域 准教授
職名
准教授

学位

  • 博士(農学) ( 北海道大学 )

研究キーワード

  • 腸管免疫

  • 食品機能化学

研究分野

  • 人文・社会 / 家政学、生活科学

  • ライフサイエンス / 食品科学

学歴

  • 北海道大学 大学院農学院 博士後期課程    

    2007年4月 - 2010年3月

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  • 京都府立大学 大学院 博士前期課程    

    2005年4月 - 2007年3月

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  • 京都府立大学農学部    

    2000年4月 - 2005年3月

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経歴

  • 岡山大学環境生命科学研究科動物栄養学研究室   准教授

    2015年5月 - 現在

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  • 北海道大学農学研究院   特任助教

    2013年11月 - 2015年4月

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  • 京都府立大学生命環境学部   Faculty of Life and Environmental Sciences   研究員

    2011年2月 - 2013年10月

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  • 北海道大学創成研究機構明治乳業寄付講座   Creative Research Institution   研究員

    2010年4月 - 2011年1月

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所属学協会

 

論文

  • Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice

    Takeshi Tsuruta, Teresia Aluoch Muhomah, Kei Sonoyama, Qui D. Nguyen, Yurika Takase, Aoi Nishijima, Shiori Himoto, Emiko Katsumata, Naoki Nishino

    Nutrition Research   93   15 - 26   2021年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.nutres.2021.06.009

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  • Consumption of indigestible saccharides and administration of Bifidobacterium pseudolongum reduce mucosal serotonin in murine colonic mucosa

    Misa Tatsuoka, Yosuke Osaki, Fumina Ohsaka, Takeshi Tsuruta, Yoshihiro Kadota, Takumi Tochio, Shingo Hino, Tatsuya Morita, Kei Sonoyama

    British Journal of Nutrition   1 - 13   2021年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Cambridge University Press (CUP)  

    <title>Abstract</title>
    SCFA increase serotonin (5-hydroxytryptamine, 5-HT) synthesis and content in the colon <italic>in vitro</italic> and <italic>ex vivo</italic>, but little is known <italic>in vivo</italic>. We tested whether dietary indigestible saccharides, utilised as a substrate to produce SCFA by gut microbiota, would increase colonic 5-HT content in mice. Male C57BL/6J mice were fed a purified diet and water supplemented with 4 % (w/v) 1-kestose (KES) for 2 weeks. Colonic 5-HT content and enterochromaffin (EC) cell numbers were lower in mice supplemented with KES than those without supplementation, while monoamine oxidase A activity and mRNA levels of tryptophan hydroxylase 1 (<italic>Tph1</italic>), chromogranin A (<italic>Chga</italic>), <italic>Slc6a4</italic> and monoamine oxidase A (<italic>Maoa</italic>) genes in the colonic mucosa, serum 5-HT concentration and total 5-HT content in the colonic contents did not differ between groups. Caecal acetate concentration and <italic>Bifidobacterium pseudolongum</italic> population were higher in KES-supplemented mice. Similar trends were observed in mice supplemented with other indigestible saccharides, that is, fructo-oligosaccharides, inulin and raffinose. Intragastric administration of live <italic>B. pseudolongum</italic> (10<sup>8</sup> colony-forming units/d) for 2 weeks reduced colonic 5-HT content and EC cell numbers. These results suggest that changes in synthesis, reuptake, catabolism and overflow of 5-HT in the colonic mucosa are not involved in the reduction of colonic 5-HT content by dietary indigestible saccharides in mice. We propose that gut microbes including <italic>B. pseudolongum</italic> could contribute to the reduction of 5-HT content in the colonic mucosa via diminishing EC cells.

    DOI: 10.1017/s0007114521001306

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  • Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides. 国際誌

    Souliphone Sivixay, Gaowa Bai, Takeshi Tsuruta, Naoki Nishino

    AIMS microbiology   7 ( 1 )   1 - 12   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Diet is considered the most influential factor in modulating the gut microbiota but how dietary protein sources differ in their modulatory effects is not well understood. In this study, soy, meat (mixture of beef and pork), and fish proteins (experiment 1) and soy, milk (casein), and egg proteins (experiment 2) were fed to rats with cellulose (CEL) and raffinose (RAF); the microbiota composition and short-chain fatty acid concentration in the cecum were determined. Egg protein feeding decreased the concentration of acetic acid and the richness and diversity of the cecum microbiota. RAF feeding increased the concentrations of acetic and propionic acids and decreased the richness and diversity of the cecum microbiota. When fed with CEL, the abundance of Ruminococcaceae and Christensenellaceae, Akkermansiaceae and Tannerellaceae, and Erysipelotrichaceae enhanced with soy protein, meat and fish proteins, and egg protein, respectively. The effects of dietary proteins diminished with RAF feeding and the abundance of Bifidobacteriaceae, Erysipelotrichaceae, and Lachnospiraceae increased and that of Ruminococcaceae and Christensenellaceae decreased regardless of the protein source. These results indicate that, although the effect of prebiotics is more robust and distinctive, dietary protein sources may influence the composition and metabolic activities of the gut microbiota. The stimulatory effects of soy, meat, and egg proteins on Christensenellaceae, Akkermansiaceae, and Erysipelotrichaceae deserve further examination to better elucidate the dietary manipulation of the gut microbiota.

    DOI: 10.3934/microbiol.2021001

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  • Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine

    Motoshi Okumura, Akihiro Hamada, Fumina Ohsaka, Takeshi Tsuruta, Tohru Hira, Kei Sonoyama

    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY   472 ( 10 )   1521 - 1532   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER HEIDELBERG  

    Serotonin (5-hydroxytryptamine [5-HT]) synthesized and released in enterochromaffin (EC) cells participates in various functions in the gastrointestinal tract by acting on a diverse range of 5-HT receptors (HTRs) expressed on smooth muscle, enteric neurons, and epithelial cells. We previously observed that genes encoding HTR2A, HTR2B, and HTR4 are expressed in murine intestinal organoids, suggesting the expression of these HTRs in intestinal epithelial cells. The present study investigated the localization of these HTRs in the murine intestine by immunofluorescence staining. HTR2A, HTR2B, and HTR4 localized in individual solitary cells in the epithelium, while HTR2C was observed in the lamina propria. In the epithelium, HTR2A, HTR2B, and HTR4 colocalized with 5-HT, and HTR4 colocalized with glucagon-like peptide 1 (GLP-1) and peptide YY (PYY). Murine intestinal organoids show a colocalization pattern that is similar to in vivo HTR2A and HTR4 with 5-HT, GLP-1, and PYY. Intraperitoneal and intragastric administration of tegaserod, an HTR4 agonist, failed to alter plasma GLP-1 levels in fasted mice. However, intragastric but not intraperitoneal administration of tegaserod reduced dietary lipid-induced increases of plasma GLP-1 levels. This action of tegaserod was inhibited by co-administration of RS39604, an HTR4 antagonist. These results suggest that murine ileal GLP-1/PYY-producing enteroendocrine (EE) cells express HTR4, while 5-HT-producing EC cells express HTR2A, HTR2B, and HTR4. In addition, the observations regarding in vivo GLP-1 secretion suggest that HTR4 signaling in ileal EE cells suppresses dietary lipid-induced GLP-1 secretion. We thus propose that EC and EE cells may interact with each other through paracrine signaling mechanisms.

    DOI: 10.1007/s00424-020-02453-7

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  • Examination of milk microbiota, fecal microbiota, and blood metabolites of Jersey cows in cool and hot seasons. 国際誌

    Qui D Nguyen, Takeshi Tsuruta, Naoki Nishino

    Animal science journal = Nihon chikusan Gakkaiho   91 ( 1 )   e13441   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Microbiota of individual cow milk, bulk tank milk, and feces of Jersey cows were examined. Samples were collected from two farms (F1 and F2) in cool (November, Nov) and hot (July, Jul) seasons. Milk yield and milk composition were similar between the two farms and between the two seasons. Prevalent taxa of the fecal microbiota, i.e. Ruminococcaceae, Bacteroidaceae, Lachnospiraceae, Rikenellaceae, and Clostridiaceae, were unaffected by the farm and season. Relative abundance of milk microbiota for Pseudomonadaceae, Enterobacteriaceae, and Streptococcaceae (F1 > F2) and Lactobacillaceae, Bifidobacteriaceae, and Cellulomonadaceae (F1 < F2) were different between the two farms, and those for Staphylococcaceae, Bacillaceae, Ruminococcaceae, and Veillonellaceae (Nov < Jul) and Methylobacteriaceae and Moraxellaceae (Nov > Jul) were different between the two seasons. The microbiota of bulk tank milk was numerically different from that of individual cow milk. Principal coordinate analysis indicated that the milk microbiota was unrelated to the fecal microbiota. The finding that relative abundance of Pseudomonadaceae and Moraxellaceae appeared greater than those reported for Holstein milk suggested that higher protein and fat content may result in a greater abundance of proteolytic and lipolytic taxa in Jersey cow milk.

    DOI: 10.1111/asj.13441

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  • Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice.

    Takeshi Tsuruta, Emiko Katsumata, Akiko Mizote, Hou Jian Jian, Teresia Aluoch Muhomah, Naoki Nishino

    Bioscience of microbiota, food and health   39 ( 3 )   188 - 196   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.

    DOI: 10.12938/bmfh.2020-012

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  • High-fat diet reduces the level of secretory immunoglobulin A coating of commensal gut microbiota. 査読

    Muhomah, T. A, Nishino, N, Katsumata, E, Haoming, W, Tsuruta, T

    Bioscience of microbiota, food and health   18   27   2019年

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  • The Relationship between Uterine, Fecal, Bedding, and Airborne Dust Microbiota from Dairy Cows and Their Environment: A Pilot Study. 査読

    Nguyen, T. T, Miyake, A, Tran, T, Tsuruta, T, Nishino, N

    9 ( 12 )   1007   2019年

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  • Rumen fluid, feces, milk, water, feed, airborne dust, and bedding microbiota in dairy farms managed by automatic milking systems. 査読

    Wu, H, Nguyen, Q. D, Tran, T. T, Tang, M. T, Tsuruta, T, Nishino, N

    Animal Science Journal   90 ( 3 )   445 - 452   2019年

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  • Dietary soy, meat, and fish proteins modulate the effects of prebiotic raffinose on composition and fermentation of gut microbiota in rats 査読

    Gaowa Bai, Takeshi Tsuruta, Naoki Nishino

    International Journal of Food Sciences and Nutrition   69 ( 4 )   480 - 487   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Taylor and Francis Ltd  

    Soy, meat (mixture of pork and beef), and fish proteins were fed to rats with and without prebiotic raffinose (RAF), and the composition and fermentation of gut microbiota were examined. Bifidobacterium spp. populations were higher, and propionic acid concentration was lower in soy protein-fed than meat protein-fed rats. Likewise, Enterobacteriaceae populations were higher in fish protein-fed rats than other rats. RAF feeding increased Bifidobacterium spp. and decreased Faecalibacterium prausnitzii populations regardless of the dietary protein source. Interactions between dietary proteins and RAF were shown for Lactobacillus spp. and Clostridium perfringens group
    the increase of Lactobacillus spp. populations by RAF was seen only for soy protein-fed rats, whereas the reduction of C. perfringens group by RAF was evident in fish and meat protein-fed rats. It is concluded that dietary proteins may differentially modulate the effects of prebiotic oligosaccharides on gut fermentation and microbiota, with differences observed between plant and animal proteins.

    DOI: 10.1080/09637486.2017.1382454

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  • Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice 査読

    Tomabechi Y, Tsuruta T, Saito S, Wabitsch M, Sonoyama K

    12 ( 2 )   433 - 439   2018年

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  • Variability, stability, and resilience of fecal microbiota in dairy cows fed whole crop corn silage 査読

    Minh Thuy Tang, Hongyan Han, Zhu Yu, Takeshi Tsuruta, Naoki Nishino

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   101 ( 16 )   6355 - 6364   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The microbiota of whole crop corn silage and feces of silage-fed dairy cows were examined. A total of 18 dairy cow feces were collected from six farms in Japan and China, and high-throughput Illumina sequencing of the V4 hypervariable region of 16S rRNA genes was performed. Lactobacillaceae were dominant in all silages, followed by Acetobacteraceae, Bacillaceae, and Enterobacteriaceae. In feces, the predominant families were Ruminococcaceae, Bacteroidaceae, Clostridiaceae, Lachnospiraceae, Rikenellaceae, and Paraprevotellaceae. Therefore, Lactobacillaceae of corn silage appeared to be eliminated in the gastrointestinal tract. Although fecal microbiota composition was similar in most samples, relative abundances of several families, such as Ruminococcaceae, Christensenellaceae, Turicibacteraceae, and Succinivibrionaceae, varied between farms and countries. In addition to the geographical location, differences in feeding management between total mixed ration feeding and separate feeding appeared to be involved in the variations. Moreover, a cow-to-cow variation for concentrateassociated families was demonstrated at the same farm; two cows showed high abundance of Succinivibrionaceae and Prevotellaceae, whereas another had a high abundance of Porphyromonadaceae. There was a negative correlation between forage-associated Ruminococcaceae and concentrate-associated Succinivibrionaceae and Prevotellaceae in 18 feces samples. Succinivibrionaceae, Prevotellaceae, p-253418B5, and Spirochaetaceae were regarded as highly variable taxa in this study. These findings help to improve our understanding of variation and similarity of the fecal microbiota of dairy cows with regard to individuals, farms, and countries. Microbiota of naturally fermented corn silage had no influence on the fecal microbiota of dairy cows.

    DOI: 10.1007/s00253-017-8348-8

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  • Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro 査読

    Ayako Aoki-Yoshida, Shinichi Saito, Takeshi Tsuruta, Arisa Ohsumi, Hinako Tsunoda, Kei Sonoyama

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   489 ( 2 )   248 - 254   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Orally administered Lactobacillus strains, including L. plantarum No.14 and L rhamnosus GG, reportedly reduce inflammatory cytokine production in mice. The present study tested our idea that circulating exosomes mediate the action of Lactobacillus strains. The lipopolysaccharide-induced production of TNF-alpha and IL-6 in vitro was attenuated in peritoneal exudate cells (PECs) isolated from C57BL/6N mice that had been fed L. plantarum No.14. When PECs were cultured for 24 h with exosomes isolated from the serum of mice fed L plantarum No.14 or L. rhamnosus GG, accumulation of both TNF-alpha and of the corresponding mRNA was lowered. Growth in the presence of these exosomes also decreased the production of TNF-alpha and IL-6 by the murine macrophage cell line RAW264.7. In contrast, supplementation with exosome-depleted serum of mice fed L plantarum No.14 or L. rhamnosus GG failed to affect the production of TNF-alpha and IL-6 by RAW264.7 cells. When PECs and RAW264.7 cells were cultured for 24 h with PKH67-labeled exosomes isolated from murine serum, fluorescent signal was observed inside the cells, suggesting that these cells incorporate serum exosomes. We propose that the anti-inflammatory activity of orally administered L plantarum No.14 and L rhamnosus GG is mediated, at least in part, by circulating exosomes. (C) 2017 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2017.05.152

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  • Comparative microbiota assessment of wilted Italian ryegrass, whole crop corn, and wilted alfalfa silage using denaturing gradient gel electrophoresis and next-generation sequencing 査読

    Kuikui Ni, Tang Thuy Minh, Tran Thi Minh Tu, Takeshi Tsuruta, Huili Pang, Naoki Nishino

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   101 ( 4 )   1385 - 1394   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The microbiota of pre-ensiled crop and silage were examined using denaturing gradient gel electrophoresis (DGGE) and next-generation sequencing (NGS). Wilted Italian ryegrass (IR), whole crop corn (WC), and wilted alfalfa (AL) silages stored for 2 months were examined. All silages contained lactic acid as a predominant fermentation product. Across the three crop species, DGGE detected 36 and 28 bands, and NGS identified 253 and 259 genera in the pre-ensiled crops and silages, respectively. The NGS demonstrated that, although lactic acid bacteria (LAB) became prevalent in all silages after 2 months of storage, the major groups were different between crops: Leuconostoc spp. and Pediococcus spp. for IR silage, Lactobacillus spp. for WC silage, and Enterococcus spp. for AL silage. The predominant silage LAB genera were also detected by DGGE, but the presence of diverse non-LAB species in pre-ensiled crops was far better detected by NGS. Likewise, good survival of Agrobacterium spp., Methylobacterium spp., and Sphingomonas spp. in IR and AL silages was demonstrated by NGS. The diversity of the microbiota described by principal coordinate analysis was similar between DGGE and NGS. Our finding that analysis of pre-ensiled crop microbiota did not help predict silage microbiota was true for both DGGE and NGS.

    DOI: 10.1007/s00253-016-7900-2

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  • Diversity of lactic acid bacteria in vegetable-based and meat-based fermented foods produced in the central region of Vietnam 査読

    Phan YTN, Tang MT, Tran TMT, Nguyen VH, Nguyen TH, Tsuruta T, Nishino N

    AIMS Microbiology   3 ( 1 )   61 - 70   2017年

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  • Dietary Casein and Soy Protein Isolate Modulate the Effects of Raffinose and Fructooligosaccharides on the Composition and Fermentation of Gut Microbiota in Rats 査読

    Gaowa Bai, Kuikui Ni, Takeshi Tsuruta, Naoki Nishino

    JOURNAL OF FOOD SCIENCE   81 ( 8 )   H2093 - H2098   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Although diet has an important influence on the composition of gut microbiota, the impact of dietary protein sources has only been studied to a minor extent. In this study, we examined the influence of different dietary protein sources regarding the effects of prebiotic oligosaccharides on the composition and metabolic activity of gut microbiota. Thirty female rats were fed casein and soy protein isolate with cellulose, raffinose (RAF), and fructooligosaccharides (FOS). Microbiota composition was examined by real-time qPCR and denaturing gradient gel electrophoresis. Dietary protein source affected cecum microbiota; acetic acid concentration and Lactobacillus spp. populations were greater with soy protein than with casein. Prebiotic oligosaccharides had distinctive effects on gut microbiota; RAF increased the acetic acid concentration and Bifidobacterium spp. populations, and FOS increased the butyric acid concentration regardless of the dietary protein. Likewise, Bifidobacterium sp., Collinsella sp., and Lactobacillus sp. were detected in microbiota of the rats fed RAF, and Bacteroides sp., Roseburia sp., and Blautia sp. were seen in microbiota of the rats fed FOS. Interactions between dietary proteins and prebiotic oligosaccharides were observed with Clostridium perfringens group populations and cecum IgA concentration. RAF and FOS decreased C. perfringens group populations in casein-fed rats, and the combination of soy protein and RAF substantially increased cecum IgA concentration. These results indicate that dietary proteins can differentially modulate the effects of prebiotic oligosaccharides on gut fermentation and microbiota, depending on the type of carbohydrate polymers involved.

    DOI: 10.1111/1750-3841.13391

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  • Organoids as an ex vivo model for studying the serotonin system in the murine small intestine and colon epithelium 査読

    Takeshi Tsuruta, Shinichi Saito, Yosuke Osaki, Akihiro Hamada, Ayako Aoki-Yoshida, Kei Sonoyama

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   474 ( 1 )   161 - 167   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Intestinal organoids were recently established as an ex vivo model of the intestinal epithelium. The present study investigated the serotonin (5-hydroxytryptamine, 5-HT) system using organoids. Organoids from murine small intestinal and colonic crypts were successfully cultured. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that small intestinal and colonic organoids express mRNAs encoding tryptophan hydroxylase-1 (TPH1) (the rate-limiting enzyme of 5-HT synthesis), serotonin reuptake transporter (SERT), 5-HT receptor (HTR)2A, HTR2B, and HTR4. SERT mRNA levels were significantly higher in the small intestine than in the colon in both the mucosal tissues and organoids, as estimated by quantitative real-time RT-PCR. Although the 5-HT concentration and levels of chromogranin A (CgA) (an enteroendocrine cell marker), TPH1, and HTR4 mRNAs were significantly higher in the colonic mucosa than the small intestinal mucosa, they were the same in small intestinal and colonic organoids. There were no significant differences in HTR2A and HTR2B mRNA levels between the small intestine and colon in either the mucosal tissues or organoids. Immunofluorescence staining showed that the number of CgA-positive cells in the colonic organoids appeared to increase upon culturing with acetate. Acetate supplementation significantly increased CgA, TPH1, and HTR4 mRNA levels in the colonic organoids. We propose that organoids are useful for investigating the 5-HT system in the intestinal epithelium, even though colonic organoids may require gut microbiota derived factors such as short-chain fatty acids. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2016.03.165

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  • Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids: Age-dependent desensitization and restoration under inflammatory conditions in mice 査読

    Masako Yajima, Shunsuke Kimura, Shinichiro Karaki, Junko Nio-Kobayashi, Takeshi Tsuruta, Atsukazu Kuwahara, Takaji Yajima, Toshihiko Iwanaga

    Physiological Reports   4 ( 7 )   1 - 11   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley-Blackwell Publishing Ltd  

    Intestinal epithelial cells sense short-chain fatty acids (SCFAs) to secrete non-neuronal acetylcholine (ACh). However, the roles of luminal SCFAs and epithelial ACh under normal and pathological conditions remain unknown. We examined ileal contractile responses to SCFAs at different ages and their mucosal cholinergic alterations under inflammatory conditions. Ileal contractile responses to SCFAs in 1-day-old pups to 7-week-old mice were compared using an isotonic transducer, and responses to an intraperitoneal injection of lipopolysaccharide (LPS) were analyzed in 7-week-old mice. The mRNA expression levels of a SCFA activate free fatty acid receptor, acetylcholinesterase (AChE), choline acetyltransferase (Chat), and choline transporter-like protein 4 (CTL4) were measured using real-time quantitative RT-PCR. AChE was analyzed by histochemical and optical enzymatic assays. Atropine-sensitive ileal contractile responses to SCFAs occurred in all 1-day-old pups, but were frequently desensitized after the weaning period. These contractile responses were not inhibited by tetrodotoxin and did not appear when the mucosal layer had been scraped off. Contractile desensitization in 7-week-old mice was abolished in the presence of the AChE inhibitor, eserine, which was consistent with increased AChE activity after weaning. Ileal contractions to SCFAs in adult mice were restored by LPS, which significantly increased the epithelial mRNA expression of Chat and CTL4. Atropine-sensitive ileal contractile responses to SCFAs constitutively occur in the newborn period, and are desensitized during developmental stages following the up-regulated expression of AChE in the villous mucosa, but are restored under inflammatory conditions possibly via the release of epithelial ACh.

    DOI: 10.14814/phy2.12759

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  • Orally Administered Salacia reticulata Extract Reduces H1N1 Influenza Clinical Symptoms in Murine Lung Tissues Putatively Due to Enhanced Natural Killer Cell Activity 査読

    Gustavo A. Romero-Perez, Masayo Egashira, Yuri Harada, Takeshi Tsuruta, Yuriko Oda, Fumitaka Ueda, Takamitsu Tsukahara, Yasuhiro Tsukamoto, Ryo Inoue

    FRONTIERS IN IMMUNOLOGY   7   1 - 9   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    Influenza is a major cause of respiratory tract infection. Although most cases do not require further hospitalization, influenza periodically causes epidemics in humans that can potentially infect and kill millions of people. To countermeasure this threat, new vaccines need to be developed annually to match emerging influenza viral strains with increased resistance to existing vaccines. Thus, there is a need for finding and developing new anti-influenza viral agents as alternatives to current treatments. Here, we tested the antiviral effects of an extract from the stems and roots of Salacia reticulata (SSRE), a plant rich in phytochemicals, such as salacinol, kotalanol, and catechins, on H1N1 influenza virus-infected mice. Following oral administration of 0.6 mg/day of SSRE, the incidence of coughing decreased in 80% of mice, and only one case of severe pulmonary inflammation was detected. Moreover, when compared with mice given Lactobacillus casei JCM1134, a strain previously shown to help increase in vitro natural killer (NK) cell activity, SSRE-administered mice showed greater and equal NK cell activity in splenocytes and pulmonary cells, respectively, at high effector cell: target cell ratios. Next, to test whether or not SSRE would exert protective effects against influenza in the absence of gut microbiota, mice were given antibiotics before being inoculated influenza virus and subsequently administered SSRE. SSRE administration induced an increase in NK cell activity in splenocytes and pulmonary cells at levels similar to those detected in mice not treated with antibiotics. Based on our results, it can be concluded that phytochemicals in the SSRE exerted protective effects against influenza infection putatively via modulation of the immune response, including enhancement of NK cell activity, although some protective effects were not necessarily through modulation of gut microbiota. Further investigation is necessary to elucidate the molecular mechanisms underlying the protective effects of SSRE against influenza infection.

    DOI: 10.3389/fimmu.2016.00115

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  • Diversity of the intestinal microbiota differently affects non-neuronal and atropine-sensitive ileal contractile responses to short-chain fatty acids in mice 査読

    Masako Yajima, Shin-Ichiro Karaki, Takeshi Tsuruta, Shunsuke Kimura, Junko Nio-Kobayashi, Atsukazu Kuwahara, Takaji Yajima

    BIOMEDICAL RESEARCH-TOKYO   37 ( 5 )   319 - 328   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Non-neuronal and atropine-sensitive ileal contractile responses to short chain fatty acids (SCFAs) are detected in the neonatal stage, and change with age or inflammatory conditions. However, the roles of luminal SCFAs in developmental changes have not yet been elucidated. We examined ileal contractile responses to SCFAs in mice colonized with different SCFA-producing intestinal microbiota under normal and inflammatory conditions. Using conventional (Conv), germ-free (GF), and gnotobiotic mice infected with Bifidobacterium (GB-bif), Propionibacterium (GB-prop), or Lactobacillus (GB-lact), ileal contractions were measured in 1-day-old neonates and 7-week-old mice using an isotonic transducer. Contractions occurred in all 1-day-old neonates, and were significantly desensitized in the adult stage in the Conv, GB-bif, and GB-prop groups, but not in the GF and GB-lact groups. An injection of lipopolysaccharide frequently restored desensitized contractions; however, the contraction rate did not change in the GF and GB-lact groups. The relative mRNA expression of a SCFA receptor (GPR43) or nicotinic acetylcholine receptor alpha 7 was weaker in the GF group (0.3-fold or 0.4-fold expression level, respectively) than in the Conv group. In conclusion, the luminal inhabitation of SCFA-producing bacteria may potentiate the regulation of non-neuronal and atropine-sensitive ileal contractile responses to SCFAs under healthy and inflammatory conditions.

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  • Intestinal epithelial cells promote secretion of leptin and adiponectin in adipocytes 査読

    Rino Ishihara, Yuki Mizuno, Akiho Miwa, Akihiro Hamada, Takeshi Tsuruta, Martin Wabitsch, Kei Sonoyama

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   458 ( 2 )   362 - 368   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Although leptin and adiponectin are the predominant adipokines, how their circulating levels are regulated is incompletely understood. The present study tested whether intestinal epithelial cells influence the expression and secretion of these adipokines by adipocytes. Leptin gene expression and secretion by cultured human primary adipocytes and Simpson-Golabi-Behmel Syndrome adipocytes increased upon coculture with human enterocytic Caco-2 cells or incubation in conditioned medium of Caco-2 cells. Although adiponectin secretion increased, its mRNA levels decreased. Tissue homogenate of the ileum (but not the jejunum, colon, or liver) of nonobese C57BL/6J mice also stimulated leptin and adiponectin secretion by cultured murine 3T3-L1 adipocytes. However, ileal homogenate of obese KK-Ay mice had no effect on leptin and adiponectin secretion. We propose that as yet unidentified humoral factors released from intestinal epithelial cells are involved in regulating circulating leptin and adiponectin levels. Decreased production of such factors may contribute to hyperphagia in KK-Ay mice. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2015.01.118

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  • Dual labeling with 5-bromo-2 '-deoxyuridine and 5-ethynyl-2 '-deoxyuridine for estimation of cell migration rate in the small intestinal epithelium 査読

    Mami Asano, Tatsuro Yamamoto, Takeshi Tsuruta, Naomichi Nishimura, Kei Sonoyama

    DEVELOPMENT GROWTH & DIFFERENTIATION   57 ( 1 )   68 - 73   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Small intestinal epithelium is a self-renewing system in which the entire sequence of cell proliferation, differentiation, and removal is coupled to cell migration along the crypt-villus axis. We examined whether dual labeling with different thymidine analogues, 5-bromo-2'-deoxyuridine (BrdU) and 5-ethynyl-2'-deoxyuridine (EdU), can be used to estimate cell migration rates on the villi of small intestines in rats. Rats received a single intraperitoneal injection of BrdU and EdU within a time interval, and signals in tissue sections were examined by immunohistochemistry and the click reaction, respectively. We successfully observed BrdU- and EdU-positive cells on the epithelium with no cross-reaction. In addition, we observed an almost complete overlapping of BrdU- and EdU-positive cells in rats administered simultaneously with BrdU and EdU. By calculating the cell migration rate by dividing the distance between the median cell positions of the distribution of BrdU- and EdU-positive cells by the time between the injection of BrdU and EdU, we estimated approximately 9 and 5m/h for the cell migration rates on the villi in the jejunum and ileum, respectively. We propose that dual labeling with BrdU and EdU within a time interval, followed by detecting with immunohistochemistry and the click reaction, respectively, is useful to estimate accurately the cell migration rate in the intestinal epithelium in a single animal.

    DOI: 10.1111/dgd.12191

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  • Adiponectin is partially associated with exosomes in mouse serum 査読

    Worrawalan Phoonsawat, Ayako Aoki-Yoshida, Takeshi Tsuruta, Kei Sonoyama

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   448 ( 3 )   261 - 266   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Exosomes are membrane vesicles 30-120 nm in diameter that are released by many cell types and carry a cargo of proteins, lipids, mRNA, and microRNA. Cultured adipocytes reportedly release exosomes that may play a role in cell-to-cell communication during the development of metabolic diseases. However, the characteristics and function of exosomes released from adipocytes in vivo remain to be elucidated. Clearly, adipocyte-derived exosomes could exist in the circulation and may be associated with adipocyte-specific proteins such as adipocytokines. We isolated exosomes from serum of mice by differential centrifugation and analyzed adiponectin, leptin, and resistin in the exosome fraction. Western blotting detected adiponectin but no leptin and only trace amounts of resistin in the exosome fraction. The adiponectin signal in the exosome fraction was decreased by proteinase K treatment and completely quenched by a combination of proteinase K and Triton X-100. Quantitative ELISA showed that the exosome fraction contains considerable amounts of adiponectin, but not leptin or resistin. The concentration of adiponectin in the serum and the ratio of adiponectin to total protein in the exosome fraction were lower in obese mice than in lean mice. These results suggest that a portion of adiponectin exists as a transmembrane protein in the exosomes in mouse serum. We propose adiponectin as a marker of exosomes released from adipocytes in vivo. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2014.04.114

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  • The evaluation of secretion volume and immunoglobulin A and G concentrations in sow colostrum from anterior to posterior teats 査読

    Shohei Ogawa, Takamitsu Tsukahara, Takeshi Tsuruta, Ryoichiro Nishibayashi, Mie Okutani, Masako Nakatani, Kaya Higashide, Shiori Iida, Nobuo Nakanishi, Kazunari Ushida, Ryo Inoue

    ANIMAL SCIENCE JOURNAL   85 ( 6 )   678 - 682   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Among domestic animals, teat order is only observed in the pig. In order to achieve the healthy growth and weaning of piglets, it is important to elucidate if volume of colostrum secretion and immunoglobulin A (IgA) and IgG concentrations differ among the teats of a sow. Nine sows were used to evaluate the difference in colostrum secretion volume (CSV) and four of these sows were assessed for IgA and IgG concentrations from each teat. Samples were collected five times during 21h following parturition. Teats were assigned anatomical locations of teat (1 to 7) from anterior to posterior. The CSV of anterior (locations 1 and 2) and middle teats (locations 3-5) was significantly higher than those of posterior teats (locations 6 and 7) throughout the experiment except for 18h post-parturition (P<0.05). The CSV of the teats at location 1 was significantly higher at most collection times than those at locations 6 and 7. A positive correlation of CSV was observed with IgA and IgG concentrations from 12h and 6h post-parturition, respectively (P<0.05). The results suggest that anterior teats secrete greater volumes of colostrum and that these tend to contain higher IgA and IgG than posteriors teats.

    DOI: 10.1111/asj.12211

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  • Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells 査読

    Takeshi Tsuruta, Ryo Inoue, Takayuki Nagino, Ryoichiro Nishibayashi, Yuko Makioka, Kazunari Ushida

    MICROBIOLOGYOPEN   2 ( 4 )   610 - 617   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR) 2-, TLR7-, and MyD88- deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs.

    DOI: 10.1002/mbo3.99

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  • The preventive effect of Bacillus subtilus strain DB9011 against experimental infection with enterotoxcemic Escherichia coli in weaning piglets 査読

    Takamitsu Tsukahara, Takeshi Tsuruta, Nobuo Nakanishi, Chie Hikita, Masami Mochizuki, Keizo Nakayama

    ANIMAL SCIENCE JOURNAL   84 ( 4 )   316 - 321   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Porcine edema disease (ED) is caused by Shiga toxin 2e-producing Escherichia coli (STEC). Post-weaned piglets often suffer from ED as a result of intestinal infection with STEC, which causes impaired growth performance and high mortality. Antimicrobial therapy is a curative treatment for piglets infected with STEC, but the emergence of antimicrobial-resistant STEC has become a serious problem for Japanese pig farmers. Therefore, an alternative strategy other than antimicrobial therapy is needed for the prevention or treatment of ED. In this study, we evaluated the effect of oral administration of Bacillus subtilisDB9011 (DB9011) to prevent the experimental infection of STEC in weaning piglets. Eight 21-day-old piglets were divided into two groups: STEC challenge with the basal diet, and STEC challenge with DB9011 supplemented diet. The challenge was carried out when the animals were 25, 26 and 27 days old using STEC contained in capsules resistant against gastric digestion. All pigs were euthanized at 36 days of age. DB9011 improved the symptoms of ED and decreased the number of STEC in the ileal digesta and feces. Accordingly, oral administration of DB9011 in weaned piglets prevents ED through the suppression of the growth of STEC in the ileum.

    DOI: 10.1111/asj.12003

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  • Oral administration of EC-12 increases the baseline gene expression of anti-viral cytokine genes, IFN-γ and TNF-α, in splenocytes and mesenteric lymph node cells of weaning piglets. 査読

    Tsuruta T, Inoue R, Tsushima T, Watanabe T, Tsukahara T, Ushida K

    Bioscience of microbiota, food and health   32 ( 4 )   123 - 8   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.12938/bmfh.32.123

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  • The G-protein on cholesterol-rich membrane microdomains mediates mucosal sensing of short-chain fatty acid and secretory response in rat colon 査読

    T. Yajima, R. Inoue, M. Yajima, T. Tsuruta, S. Karaki, T. Hira, A. Kuwahara

    ACTA PHYSIOLOGICA   203 ( 3 )   381 - 389   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Aim: Short-chain fatty acids (SCFA) stimulate colonic contraction and secretion, which are mediated by an enteric reflex via a mucosal sensing and cholinergic mechanisms. The involvement of G-protein signal transduction was examined in the secretory response to luminal propionate sensing in rat distal colon.
    Methods: Mucosa-submucosa and mucosa preparations were used to measure short-circuit current (I-sc) and acetylcholine (ACh) release respectively. Cholesterol-rich membrane microdomains, lipid rafts/caveolae, were fractionated using a sucrose gradient ultra-centrifugation after detergent-free extraction of the isolated colonic crypt.
    Results: Luminal addition of methyl-beta-cyclodextrin (10 mm) and mastoparan (30 mu m), lipid rafts/caveolae disruptors, significantly inhibited luminal propionate-induced (0.5 mm) increases in I-sc, but did not affect increases in I-sc induced by serosal ACh (0.05 mm) or electrical field stimulation (EFS). Luminal addition of YM-254890 (10 mu m), a G alpha(q/11)-selective inhibitor, markedly inhibited propionate-induced increase in I-sc, but did not affect I-sc responses to ACh and EFS. Both methyl-beta-cyclodextrin and YM-254890 significantly inhibited luminal propionate-induced non-neuronal release of ACh from colonocytes. Real-time PCR demonstrated that in mRNA expression of SCFA receptors, GPR 43 was far higher than that of GPR41 in the colon. Western blotting analysis revealed that the cholesterol-rich membrane microdomains that fractionated from colonic crypt cells were associated with caveolin-1, flotillin-1 and G alpha(q/11), but not GPR43. Uncoupling of G alpha(q/11) from flotillin-1 in lipid rafts occurred under desensitization of the I-sc response to propionate.
    Conclusions: These data demonstrate that the secretory response to luminal propionate in rat colon is mediated by G-protein on cholesterol-rich membrane microdomains, provably via G alpha(q/11).

    DOI: 10.1111/j.1748-1716.2011.02331.x

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  • Commensal bacteria coated by secretory immunoglobulin A and immunoglobulin G in the gastro-intestinal tract of pigs and calves. 査読

    Tsuruta T, Inoue R, Tsukahara T, Hara H, Yajima T

    Animal Science Journal   83   799 - 804   2011年

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  • Development of a Method for the Identification of S-IgA-Coated Bacterial Composition in Mouse and Human Feces 査読

    Takeshi Tsuruta, Ryo Inoue, Toshihiko Iwanaga, Hiroshi Hara, Takaji Yajima

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 5 )   968 - 973   2010年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgA-coated bacteria has been established. To identify S-IgA-coated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.

    DOI: 10.1271/bbb.90801

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  • Long-term oral administration of cows’ milk improves insulin sensitivity in rats fed a high-sucrose diet. 査読

    Matsumoto M, Inoue R, Tsuruta T, Hara H, Yajima T

    British Journal of Nutrition   102 ( 9 )   1324 - 1333   2009年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1017/S0007114509990365

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  • The amount of secreted IgA may not determine the secretory IgA coating ratio of gastrointestinal bacteria 査読

    Takeshi Tsuruta, Ryo Inoue, Iyori Nojima, Takamitsu Tsukahara, Hiroshi Hara, Takaji Yajima

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   56 ( 2 )   185 - 189   2009年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    It is reported that some, but not all, bacteria in human faeces are coated with secretory immunoglobulin A (S-IgA). We evaluated the proportion of S-IgA-coated bacteria to total intestinal bacteria (S-IgA coating ratio) in the gastrointestinal tract of two different strains of mice supplied by two different suppliers. The S-IgA coating ratio was significantly different in each gastrointestinal segment and between mouse suppliers. The amount of non-bacteria-bound IgA (free IgA) in each gastrointestinal segment indicated that this difference in the S-IgA coating ratio might not be due to the amount of secreted IgA. Furthermore, immunoblotting analysis revealed that only a small amount of IgA (&lt; 5% to free-IgA) was used for the coating. This indicates that, although sufficient S-IgA was secreted to coat the entire intestinal population of bacteria, only some part of the bacteria were coated with S-IgA. This study suggests that the amount of luminal S-IgA may not determine the S-IgA coating ratio, and that the amount of IgA coating intestinal commensal bacteria is very small.

    DOI: 10.1111/j.1574-695X.2009.00568.x

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  • A cell preparation of Enterococcus faecalis strain EC-12 stimulates the luminal immunoglobulin A secretion in juvenile calves 査読

    Takeshi Tsuruta, Ryo Inoue, Takamitsu Tsukahara, Noritaka Matsubara, Masayuki Hamasaki, Kazunari Ushida

    ANIMAL SCIENCE JOURNAL   80 ( 2 )   206 - 211   2009年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    The immune system in juvenile calves is immature, so calves are susceptible to several diarrheal and respiratory diseases. Oral administration of lactic acid bacteria (LAB) is known to improve the growth performance and prevent diarrheal and respiratory diseases by stimulating the immune system in juvenile calves. Most of the immunostimulation by LAB is achieved by their cell wall components, and therefore we evaluated the immunostimulation of the cell preparation of Enterococcus faecalis strain EC-12 (EC-12) in juvenile calves in a clinical field. Twenty-nine 1-week old calves were used. Fourteen calves were administered 0.2% (w/w) of an EC-12 preparation that supplemented a milk replacer, and other calves were not supplemented. Feces and serum was collected at day 0, 7 and 49 after the administration to measure the IgA and IgG concentration. The fecal IgA concentration was increased by EC-12 administration at day 49, and the serum IgA concentration was also increased at day 7. These results suggested that oral administration of EC-12 in juvenile calves might have an immunostimulatory effect and provide earlier recovery of IgA levels in mucosal immunity.

    DOI: 10.1111/j.1740-0929.2008.00621.x

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  • LONG-TERM ORAL FEEDINGS OF COW MILK AND WITH LUTEIN INCREASE THE VOLUNTARY RUNNING DISTANCE IN RAT 査読

    Megumi Matsumoto, Masahito Hagio, Takeshi Tsuruta, Ryo Inoue, Hiroshi Hara, Takaji Yajima

    ANNALS OF NUTRITION AND METABOLISM   55   430 - 430   2009年

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    記述言語:英語   出版者・発行元:KARGER  

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  • Postnatal changes in the expression of genes for cryptdins 1-6 and the role of luminal bacteria in cryptdin gene expression in mouse small intestine 査読

    Ryo Inoue, Takeshi Tsuruta, Iyori Nojima, Keizo Nakayama, Takamitsu Tsukahara, Takaji Yajima

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   52 ( 3 )   407 - 416   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Although there have been many fascinating studies on cryptdins, the information for each cryptdin isoform was not completely provided. In this study, the postnatal changes in the gene expression of cryptdin 1-6 were evaluated, and the patterns of change were compared between conventional and germ-free mice. Two patterns of postnatal change were observed: gene expression of cryptdins 1, 3 and 6 increased gradually, and that of cryptdins 2 and 5 increased rapidly. Gene expression of cryptdin 4 increased gradually in the ileum but rapidly in the jejunum. Conventional mice showed significantly higher gene expression for all isoforms than germ-free mice. Interestingly, the difference in the gene expression for cryptdin 2, 4 and 5 between the jejunum and ileum seemed to be increased by the presence of the luminal bacteria. The results indicate that cryptdin isoforms develop differently depending on the isoform type, and that the gene expression of all cryptdin isoforms was affected by the presence of the luminal bacteria.

    DOI: 10.1111/j.1574-695X.2008.00390.x

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▼全件表示

MISC

  • 環状四糖(CNN)の摂取が高脂肪食誘導性肥満マウスのインスリン抵抗性に及ぼす影響

    鶴田剛司, 溝手晶子, 塩津萌々子, 佐相晴香, 西野直樹

    日本農芸化学会大会講演要旨集(Web)   2021   2021年

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  • 高脂肪食の摂取にともなう腸管IgAの腸内細菌に対する反応性の低下と肥満病態の関係性

    鶴田剛司, 園山慶, 西野直樹

    日本栄養・食糧学会大会講演要旨集   74th   2020年

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  • 腸管免疫(分泌型IgA)と腸内細菌の相互作用に油脂の摂取が及ぼす影響

    鶴田剛司

    日本栄養・食糧学会中国・四国支部大会講演要旨集   53rd (CD-ROM)   2020年

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    小田沙也可, 鶴田剛司, 西野直樹

    日本草地学会誌   65   2019年

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  • 牛乳に含まれる細胞外小胞がヒト大腸がん細胞株の増殖活性に及ぼす影響

    高瀬友里加, 鶴田剛司, 西野直樹

    日本栄養・食糧学会大会講演要旨集   73rd   2019年

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  • 牛乳中の細胞外小胞がAOM/DSS大腸がん誘導マウスの腫瘍形成に及ぼす影響

    椛島奈津美, 西野直樹, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集   73rd   2019年

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  • 牛乳中の細胞外小胞が食餌誘導性肥満マウスの耐糖能異常に及ぼす影響

    樋本栞, 西野直樹, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集   73rd   2019年

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  • シクロニゲロシルニゲロース摂取がDSS腸炎に及ぼす影響

    勝又恵実子, 西野直樹, 中村修治, 角田省二, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集   73rd   2019年

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  • 分泌型免疫グロブリンAの腸内細菌への結合活性は高脂肪食の摂取により弱まる

    鶴田剛司, MUHOMAH Teresia Aluoch, 園山慶, 西野直樹

    岡山実験動物研究会報   ( 35 )   2019年

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  • 組換え型REG3βの投与は食餌誘導性肥満マウスの末梢組織炎症およびインスリン抵抗性を緩和する

    鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会大会講演要旨集   73rd   2019年

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  • 腸内細菌と宿主のクロストークを媒介するエクソソーム

    園山慶, 青木(吉田)綾子, 鶴田剛司

    日本農芸化学会大会講演要旨集(Web)   2019   2019年

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  • 牛乳中の細胞外小胞によるヒト大腸がん細胞株Caco-2細胞の増殖抑制作用

    鶴田剛司, 福田久仁香, 樋本栞, 勝又恵実子, 園山慶, 西野直樹

    日本栄養・食糧学会大会講演要旨集   72nd   2018年

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞はマウスの内臓脂肪蓄積を抑制する

    伊藤洋平, 鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会中国・四国支部大会講演要旨集   51st   2018年

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞は内臓脂肪組織の脂肪蓄積抑制を媒介する

    伊藤洋平, 鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会大会講演要旨集   72nd   2018年

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  • REG3βが食餌誘導性肥満およびインスリン抵抗性に及ぼす影響

    磯田康太朗, 鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会大会講演要旨集   72nd   2018年

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  • マクロファージが分泌する細胞外小胞は乳酸菌の抗肥満効果を媒介する

    伊藤洋平, 鶴田剛司, 西野直樹, MUHOMAH Terry, 園山慶

    岡山実験動物研究会報   ( 34 )   2018年

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  • 腸内細菌の健康機能を媒介する細胞外小胞

    園山慶, 鶴田剛司, 青木(吉田)綾子

    日本栄養・食糧学会大会講演要旨集   72nd   2018年

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  • 乳酸菌を貪食したマクロファージが放出する細胞外小胞の脂肪細胞および骨格筋細胞への取り込み

    鶴田剛司, 伊藤洋平, 西野直樹, MUHOMAH Teresia Aluoch, 勝又恵実子, 園山慶

    岡山実験動物研究会報   ( 34 )   2018年

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  • シクロニゲロシルニゲロースの摂取が分泌型免疫グロブリンAの分泌および腸内細菌への反応性に及ぼす影響

    勝又恵実子, 西野直樹, TERESIA Muhomah, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集   72nd   2018年

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  • 分泌型免疫グロブリンAの腸内細菌への結合活性は高脂肪食の摂取により弱まる

    MUHOMAH Teresia Aluoch, 西野直樹, 園山慶, 桑高佳穂, 鶴田剛司

    日本栄養・食糧学会中国・四国支部大会講演要旨集   51st   2018年

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  • 組換え型REG3βの投与は高脂肪食摂取マウスにおける代謝異常を抑制する

    磯田康太朗, 鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会中国・四国支部大会講演要旨集   51st   2018年

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  • プロバイオティック乳酸菌の健康機能が生体内で伝達されるメカニズムの解明

    鶴田剛司

    東洋食品研究所研究報告書   ( 31 )   2017年

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  • プロバイオティクスの健康機能を媒介する循環血中のエクソソーム 腸内細菌叢と宿主の新たなクロストークシステムか?

    園山慶, 青木(吉田)綾子, 鶴田剛司

    化学と生物   56 ( 1 )   2017年

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞は細胞種選択的に細胞の栄養代謝に影響を及ぼす

    鶴田剛司, 伊藤洋平, MUHOMAH Terry, 西野直樹, 園山慶

    日本栄養・食糧学会大会講演要旨集   71st   2017年

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  • 高アミロース含有トウモロコシ澱粉のメタボリックシンドローム改善機序としての小腸急性期タンパク

    鶴田剛司, 鶴田剛司

    飯島藤十郎記念食品科学振興財団年報   31   2016年

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  • 牛乳中の細胞外小胞によるヒト大腸癌細胞株Caco-2細胞の増殖抑制作用

    福田久仁香, 西野直樹, 園山慶, 鶴田剛司

    日本栄養・食糧学会中国・四国支部大会講演要旨集   49th   2016年

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  • マウスの小腸粘膜上皮におけるペプチドYY産生細胞はセロトニン受容体を発現する

    濱田晃弘, 尾崎洋輔, 斉藤伸一, 鶴田剛司, 青木(吉田)綾子, 園山慶

    日本栄養・食糧学会大会講演要旨集   70th   2016年

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  • DGGEおよびNGSによるイタリアンライグラス,アルファルファおよびトウモロコシサイレージの細菌叢解析

    西野直樹, NI Kuikui, YEN Ngoc Phan Thi, BAIYILA Wu, 鶴田剛司

    日本畜産学会大会講演要旨   121st   2016年

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞は白色脂肪細胞の脂肪蓄積を抑制する

    鶴田剛司, 伊藤洋平, 西野直樹, MUHOMAH Terry, 園山慶

    日本栄養・食糧学会中国・四国支部大会講演要旨集   49th   2016年

     詳細を見る

  • 腸内細菌叢の情報を宿主に伝える血中エキソソームのmiRNA

    園山慶, 鶴田剛司, 青木(吉田)綾子

    日本栄養・食糧学会大会講演要旨集   70th   2016年

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  • 短期間の高脂肪食摂取によってマウスの腹腔内脂肪におけるリポ多糖結合タンパクの発現が増加する

    関真実, 三輪秋穂, 鶴田剛司, 園山慶

    日本農芸化学会大会講演要旨集(Web)   2016   2016年

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  • 分泌型免疫グロブリンAが認識する腸内細菌種の同定

    鶴田剛司

    岡山大学農学部学術報告(Web)   105   2016年

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  • 短鎖脂肪酸がマウス結腸オルガノイドのセロトニンシステムにおよぼす影響

    尾崎洋輔, 斉藤伸一, 濱田晃弘, 鶴田剛司, 園山慶

    日本栄養・食糧学会大会講演要旨集   70th   2016年

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  • 短鎖脂肪酸がマウス結腸オルガノイドの腸内分泌細胞分化に及ぼす影響

    尾崎洋輔, 斉藤伸一, 濱田晃弘, 鶴田剛司, 園山慶

    日本農芸化学会大会講演要旨集(Web)   2016   2016年

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  • R-spondinおよびNogginの発現細胞株の作成とマウス腸管オルガノイドへの応用

    尾崎洋輔, 鶴田剛司, 斉藤伸一, 園山慶

    日本農芸化学会北海道支部講演会講演要旨   2015   2015年

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  • 食餌タンパク質およびオリゴ糖による盲腸内短鎖脂肪酸濃度と細菌フローラの変動

    白高娃, 鶴田剛司, 西野直樹

    日本栄養・食糧学会中国・四国支部大会講演要旨集   48th   2015年

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  • マウス小腸陰窩オルガノイドにおけるセロトニン自己分泌刺激

    濱田晃弘, 鶴田剛司, 青木(吉田)綾子, 園山慶

    日本農芸化学会大会講演要旨集(Web)   2015   2015年

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  • 血清エキソソームはフラクトオリゴ糖の炎症抑制作用を媒介するか?

    角田妃菜子, 大角有里沙, 三輪明穂, 青木(吉田)綾子, 鶴田剛司, 園山慶

    日本栄養・食糧学会大会講演要旨集   68th   2014年

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  • プロバイオティクスの炎症抑制作用を媒介するエキソソーム

    青木(吉田)綾子, 大角有里沙, 鶴田剛司, 園山慶

    日本農芸化学会大会講演要旨集(Web)   2014   2014年

     詳細を見る

  • 消化管内の健康と子牛の下痢症対策 子牛の消化管細菌叢とIgAの関係

    鶴田剛司, 牛田一成

    臨床獣医   31 ( 12 )   2013年

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  • 消化管における酪酸の粘液透過性とその影響

    塚原隆充, 塚原隆充, 鶴田剛司, 鶴田剛司, 松川典子, 牛田一成, 落合邦康

    日本栄養・食糧学会大会講演要旨集   66th   2012年

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  • 腸内細菌叢調節を介したサラシア属植物エキスのインフルエンザ症状軽減作用

    植田文教, 小田由里子, 鶴田剛司, 柿沼千早, 井上亮, 牛田一成, 塚本康浩

    日本食品科学工学会大会講演集   59th   2012年

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  • ブタ及びウシの各消化管部位に存在するS-IgA及びIgGに被覆された細菌

    鶴田剛司, 井上亮, 塚原隆允, 中本光則, 原博, 矢島高二, 牛田一成

    日本畜産学会大会講演要旨   114th   2011年

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  • 短鎖脂肪酸が腸上皮杯細胞の表面糖鎖に及ぼす影響

    野島伊世里, 井上亮, 鶴田剛司, 原博, 矢島高二

    日本農芸化学会大会講演要旨集   2009   2009年

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  • 様々な動物種の各腸管部位に存在するS-IgAコートされた腸内細菌

    鶴田剛司, 井上亮, 野島伊世里, 塚原隆充, 中本光則, 原博, 矢島高二

    日本農芸化学会大会講演要旨集   2008   2008年

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▼全件表示

共同研究・競争的資金等の研究

  • 腸管IgAと腸内細菌の結合弱化の予防を基軸としたインスリン抵抗性の制御戦略

    研究課題/領域番号:20K11580  2020年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    鶴田 剛司

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

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  • 細菌叢と真菌叢の統合的解析で切り拓くサイレージ微生物制御の新機軸

    研究課題/領域番号:19H03106  2019年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    西野 直樹, 鶴田 剛司

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    配分額:17290000円 ( 直接経費:13300000円 、 間接経費:3990000円 )

    暖地型牧草、食品副産物および混合飼料(TMR)を対象とし、実験室規模でサイレージを調製した。貯蔵温度を3水準(10℃、25℃、40℃)設定し、1週間、2週間、2ヶ月および6ヶ月後に開封して、発酵生成物、好気的安定性および微生物叢を調査した。冷涼(10℃)および暑熱(40℃)環境での貯蔵が、貯蔵性と好気的安定性をどれほど変動させるかを課題とした。細菌叢のアンプリコン解析は16S rRNA遺伝子のV4領域を、真菌叢のそれは26S rRNA遺伝子のITS領域を対象として行っている。
    微生物叢解析まで終了したのは、食品副産物のサイレージである。Acinetobacter spp.がLactobacillus spp.より優勢でありながら、乳酸優勢の発酵特性を示すという興味深い知見が得られるとともに、貯蔵期間が長くなるとBacillus spp.が増加して乳酸生成を維持するという新規性に富む知見も得られた。一方、真菌叢は貯蔵性および好気的安定性と関連する変化を示しておらず、細菌叢と真菌叢のネットワーク解析も今後の課題である。暖地型牧草サイレージは酢酸優勢の発酵特性を示すことが多いが、それが十分再現されたサンプルが得られており、現在細菌叢および真菌叢のアンプリコン解析を進めている。これらのサイレージから培養法による有用乳酸菌の分離および選抜も進めており、意図的に変敗させたサンプルから酵母およびカビの分離も行っている。実規模サイレージのサンプリングも行っており、それらの微生物叢解析も予定通り進めている。

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  • 大腸上皮がん細胞の増殖を抑制する牛乳中の細胞外小胞の解析

    2017年 - 2019年

    文部科学省  科学研究費補助金(若手研究B) 

    鶴田 剛司

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    担当区分:研究代表者  資金種別:競争的資金

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  • プロバイオティクスの刺激情報を伝達する貪食細胞エキソソームの解析

    2015年 - 2016年

    文部科学省  科学研究費補助金(若手研究B) 

    鶴田 剛司

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    担当区分:研究代表者  資金種別:競争的資金

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担当授業科目

  • Laboratory in Animal Science 3 (2021年度) 1・2学期  - 金5,金6,金7,金8

  • コース演習4 (2021年度) 3・4学期  - その他

  • 動物栄養学演習 (2021年度) 前期  - その他

  • 動物栄養学演習 (2021年度) 後期  - その他

  • 動物栄養学演習 (2021年度) 後期  - その他

  • 動物栄養学演習 (2021年度) 前期  - その他

  • 動物栄養学特論 (2021年度) 後期  - 火1,火2

  • 動物栄養調節学 (2021年度) 前期  - その他

  • 卒業論文 (2021年度) 1~4学期  - その他

  • 応用動物科学コース実験3 (2021年度) 1・2学期  - 金5,金6,金7,金8

  • 応用動物科学コース実験3-1 (2021年度) 第1学期  - 金5,金6,金7,金8

  • 応用動物科学コース実験3-2 (2021年度) 第2学期  - 金5,金6,金7,金8

  • 栄養生化学2 (2021年度) 第4学期  - 金5,金6

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 研究科目演習1 (2021年度) 1・2学期  - その他

  • 研究科目演習2 (2021年度) 3・4学期  - その他

  • 食品栄養学1 (2021年度) 第1学期  - 月3,月4

  • 食品栄養学2 (2021年度) 第2学期  - 月3,月4

  • Laboratory in Animal Science 3-1 (2020年度) 第1学期  - 金5,金6,金7,金8

  • Laboratory in Animal Science 3-2 (2020年度) 第2学期  - 金5,金6,金7,金8

  • コース演習4 (2020年度) 3・4学期  - その他

  • 動物栄養学演習 (2020年度) 前期  - その他

  • 動物栄養学演習 (2020年度) 後期  - その他

  • 動物栄養学演習 (2020年度) 後期  - その他

  • 動物栄養学演習 (2020年度) 前期  - その他

  • 動物栄養学特論 (2020年度) 後期  - 火1,火2

  • 動物栄養調節学 (2020年度) 前期  - その他

  • 卒業論文 (2020年度) 1~4学期  - その他

  • 応用動物科学コース実験3-1 (2020年度) 第1学期  - 金5,金6,金7,金8

  • 応用動物科学コース実験3-2 (2020年度) 第2学期  - 金5,金6,金7,金8

  • 栄養生化学2 (2020年度) 第4学期  - 水5,水6

  • 生物生産科学特別研究 (2020年度) 通年  - その他

  • 研究科目演習1 (2020年度) 1・2学期  - その他

  • 研究科目演習2 (2020年度) 3・4学期  - その他

  • 食品栄養学1 (2020年度) 第1学期  - 月3,月4

  • 食品栄養学2 (2020年度) 第2学期  - 月3,月4

▼全件表示