Faculty Profiles - YAMADA Hiroshi

写真a

YAMADA Hiroshi

Organization

Faculty of Medicine, Dentistry and Pharmaceutical Sciences Associate Professor

Homepage

http://www.okayama-u.ac.jp/user/biochem/index.html

External link

Degree

博士（理学） ( 1997.3 広島大学 )

Research Interests

クライオ電子顕微鏡
microvesicleを介した内在性内分泌制御
cytoskeleton
cell biology
biochemistry
biophysics
membrane traffic
腎臓の血液濾過の分子生理
マラリア原虫の細胞生理
がん細胞の遊走、浸潤

Research Areas

Life Science / Physiology
Life Science / Structural biochemistry / 電子顕微鏡学
Life Science / Functional biochemistry
Life Science / Cell biology
Life Science / Parasitology

Education

Hiroshima University 理学研究科遺伝子科学専攻

1995 - 1997

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大阪大学大学院工学研究科博士前期課程醗酵工学専攻

1991 - 1993

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Osaka University 工学部醗酵工学科

1987 - 1991

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Research History

Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Department of Biochemistry Research Professor

2023.6

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Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Department of Biochemistry Associate Professor

2011.4

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岡山大学大学院医歯薬学総合研究科・講師

2005.9 - 2011.3

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岡山大学大学院医歯薬学総合研究科・助手（配置換）

2005.4 - 2005.8

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米国イェール大学医学部細胞生物学講座 (Pietro DeCamilli教授）客員研究員

2002.10 - 2003.3

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岡山大学大学院医歯学総合研究科・助手（配置換）

2001.4 - 2005.3

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岡山大学医学部・助手

2000.5 - 2001.3

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岡山大学大学院自然科学研究科・講師・中核的研究機関研究員

1999.10 - 2000.4

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Osaka University The Institute of Scientific and Industrial Research

1997 - 1999

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Professional Memberships

The Society for Biotechnology, Japan

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THE JAPANESE SOCIETY OF PARASITOLOGY

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THE JAPANESE BIOCHEMICAL SOCIETY

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JAPAN SOCIETY FOR CELL BIOLOGY

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Papers

Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes. Reviewed International coauthorship International journal

The Mon La, Hiromi Tachibana, Shun-Ai Li, Tadashi Abe, Sayaka Seiriki, Hikaru Nagaoka, Eizo Takashima, Tetsuya Takeda, Daisuke Ogawa, Shin-Ichi Makino, Katsuhiko Asanuma, Masami Watanabe, Xuefei Tian, Shuta Ishibe, Ayuko Sakane, Takuya Sasaki, Jun Wada, Kohji Takei, Hiroshi Yamada

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34 ( 12 ) 16449 - 16463 2020.12

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. Dynamin 1 is also expressed in the kidney; however, its physiological significance to this organ remains unknown. Here, we show that dynamin 1 is crucial for microtubule organization and stabilization in glomerular podocytes. By immunofluorescence and immunoelectron microscopy, dynamin 1 was concentrated at microtubules at primary processes in rat podocytes. By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. In dynamin 1-depleted MPCs by RNAi, α-tubulin showed a dispersed linear filament-like localization, and microtubule bundles were rarely observed. Furthermore, dynamin 1 depletion resulted in the formation of discontinuous, short acetylated α-tubulin fragments, and the decrease of microtubule-rich protrusions. Dynamins 1 and 2 double-knockout podocytes showed dispersed acetylated α-tubulin and rare protrusions. In vitro, dynamin 1 polymerized around microtubules and cross-linked them into bundles, and increased their resistance to the disassembly-inducing reagents Ca2+ and podophyllotoxin. In addition, overexpression and depletion of dynamin 1 in MPCs increased and decreased the nocodazole resistance of microtubules, respectively. These results suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules.

DOI： 10.1096/fj.202001240RR

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.202001240RR
Vesicular Glutamate Transporter 3 Is Involved in Glutamatergic Signalling in Podocytes Reviewed

Naoko Nishii, Tomoko Kawai, Hiroki Yasuoka, Tadashi Abe, Nanami Tatsumi, Yuika Harada, Takaaki Miyaji, Shunai Li, Moemi Tsukano, Masami Watanabe, Daisuke Ogawa, Jun Wada, Kohji Takei, Hiroshi Yamada

International Journal of Molecular Sciences 26 ( 6 ) 2025.3

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

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Distribution and Incorporation of Extracellular Vesicles into Chondrocytes and Synoviocytes Reviewed

Takashi Ohtsuki, Ikumi Sato, Ren Takashita, Shintaro Kodama, Kentaro Ikemura, Gabriel Opoku, Shogo Watanabe, Takayuki Furumatsu, Hiroshi Yamada, Mitsuru Ando, Kazunari Akiyoshi, Keiichiro Nishida, Satoshi Hirohata

International Journal of Molecular Sciences 25 11942 2024.11

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.3390/ijms252211942

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Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes. Reviewed

Yamada, H, Osaka, H, Tatsumi, N, Araki, M, Abe, T, Kaihara, K, Takahashi, K, Takashima, E, Uchihashi, T, Naruse, K, Takei, K

Cells 13 1373 2024.8

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.3390/cells13161373

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The microtubule-dynamin binding inhibitor peptide PHDP5 rescues spatial learning and memory deficits in Alzheimer’s disease model mice Reviewed

Chia-Jung Chang, Zacharie Taoufiq, Hiroshi Yamada, Kohji Takei, Takami Tomiyama, Tomohiro Umeda, Tetsuya Hori, Tomoyuki Takahashi

Brain Research 148987 - 148987 2024.5

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.brainres.2024.148987

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Structural basis of Irgb6 inactivation by Toxoplasma gondii through the phosphorylation of switch I Reviewed

Hiromichi Okuma, Yumiko Saijo‐Hamano, Hiroshi Yamada, Aalaa Alrahman Sherif, Emi Hashizaki, Naoki Sakai, Takaaki Kato, Tsuyoshi Imasaki, Satoshi Kikkawa, Eriko Nitta, Miwa Sasai, Tadashi Abe, Fuminori Sugihara, Yoshimasa Maniwa, Hidetaka Kosako, Kohji Takei, Daron M. Standley, Masahiro Yamamoto, Ryo Nitta

Genes to Cells 29 ( 1 ) 17 - 38 2024.1

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Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Abstract

Irgb6 is a priming immune‐related GTPase (IRG) that counteracts Toxoplasma gondii. It is known to be recruited to the low virulent type II T. gondii parasitophorous vacuole (PV), initiating cell‐autonomous immunity. However, the molecular mechanism by which immunity‐related GTPases become inactivated after the parasite infection remains obscure. Here, we found that Thr95 of Irgb6 is prominently phosphorylated in response to low virulent type II T. gondii infection. We observed that a phosphomimetic T95D mutation in Irgb6 impaired its localization to the PV and exhibited reduced GTPase activity in vitro. Structural analysis unveiled an atypical conformation of nucleotide‐free Irgb6‐T95D, resulting from a conformational change in the G‐domain that allosterically modified the PV membrane‐binding interface. In silico docking corroborated the disruption of the physiological membrane binding site. These findings provide novel insights into a T. gondii‐induced allosteric inactivation mechanism of Irgb6.

DOI： 10.1111/gtc.13080

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Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells Reviewed

Haymar Win, Jianzhen Li, Tadashi Abe, Hiroshi Yamada, Takumi Higaki, Yasutomo Nasu, Masami Watanabe, Kohji Takei, Tetsuya Takeda

Journal of Cell Science jcs.260827 2023.5

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1242/jcs.260827

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Recruitment of Irgb6 to the membrane is a direct trigger for membrane deformation Reviewed International journal

Hiroshi Yamada, Tadashi Abe, Hikaru Nagaoka, Eizo Takashima, Ryo Nitta, Masahiro Yamamoto, Kohji Takei

Frontiers in Cellular and Infection Microbiology 12 992198 - 992198 2022.9

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers Media SA

Irgb6 is a member of interferon γ-induced immunity related GTPase (IRG), and one of twenty “effector” IRGs, which coordinately attack parasitophorous vacuole membrane (PVM), causing death of intracellular pathogen. Although Irgb6 plays a pivotal role as a pioneer in the process of PVM disruption, the direct effect of Irgb6 on membrane remained to be elucidated. Here, we utilized artificial lipid membranes to reconstitute Irgb6-membrane interaction in vitro, and revealed that Irgb6 directly deformed the membranes. Liposomes incubated with recombinant Irgb6 were drastically deformed generating massive tubular protrusions in the absence of guanine nucleotide, or with GMP-PNP. Liposome deformation was abolished by incubating with Irgb6-K275A/R371A, point mutations at membrane targeting residues. The membrane tubules generated by Irgb6 were mostly disappeared by the addition of GTP or GDP, which are caused by detachment of Irgb6 from membrane. Binding of Irgb6 to the membrane, which was reconstituted in vitro using lipid monolayer, was stimulated at GTP-bound state. Irgb6 GTPase activity was stimulated by the presence of liposomes more than eightfold. Irgb6 GTPase activity in the absence of membrane was also slightly stimulated, by lowering ionic strength, or by increasing protein concentration, indicating synergistic stimulation of the GTPase activity. These results suggest that membrane targeting of Irgb6 and resulting membrane deformation does not require GTP, but converting into GTP-bound state is crucial for detaching Irgb6 from the membrane, which might coincident with local membrane disruption.

DOI： 10.3389/fcimb.2022.992198

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Dynamin: molecular scissors for membrane fission

Tetsuya Takeda, Hiroshi Yamada, Kohji Takei

Plasma Membrane Shaping 77 - 90 2022.9

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Publishing type：Part of collection (book) Publisher：Elsevier

DOI： 10.1016/b978-0-323-89911-6.00023-6

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The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes Reviewed International journal

Eriko Hamasaki, Natsuki Wakita, Hiroki Yasuoka, Hikaru Nagaoka, Masayuki Morita, Eizo Takashima, Takayuki Uchihashi, Tetsuya Takeda, Tadashi Abe, Ji-Won Lee, Tadahiro Iimura, Moin A Saleem, Naohisa Ogo, Akira Asai, Akihiro Narita, Kohji Takei, Hiroshi Yamada

Frontiers in Cell and Developmental Biology 10 1 - 12 2022.5

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Dynamin is an endocytic protein that functions in vesicle formation by scission of invaginated membranes. Dynamin maintains the structure of foot processes in glomerular podocytes by directly and indirectly interacting with actin filaments. However, molecular mechanisms underlying dynamin-mediated actin regulation are largely unknown. Here, biochemical and cell biological experiments were conducted to uncover how dynamin modulates interactions between membranes and actin in human podocytes. Actin-bundling, membrane tubulating, and GTPase activities of dynamin were examined in vitro using recombinant dynamin 2-wild-type (WT) or dynamin 2-K562E, which is a mutant found in Charcot-Marie-Tooth patients. Dynamin 2-WT and dynamin 2-K562E led to the formation of prominent actin bundles with constant diameters. Whereas liposomes incubated with dynamin 2-WT resulted in tubule formation, dynamin 2-K562E reduced tubulation. Actin filaments and liposomes stimulated dynamin 2-WT GTPase activity by 6- and 20-fold, respectively. Actin-filaments, but not liposomes, stimulated dynamin 2-K562E GTPase activity by 4-fold. Self-assembly-dependent GTPase activity of dynamin 2-K562E was reduced to one-third compared to that of dynamin 2-WT. Incubation of liposomes and actin with dynamin 2-WT led to the formation of thick actin bundles, which often bound to liposomes. The interaction between lipid membranes and actin bundles by dynamin 2-K562E was lower than that by dynamin 2-WT. Dynamin 2-WT partially colocalized with stress fibers and actin bundles based on double immunofluorescence of human podocytes. Dynamin 2-K562E expression resulted in decreased stress fiber density and the formation of aberrant actin clusters. Dynamin 2-K562E colocalized with α-actinin-4 in aberrant actin clusters. Reformation of stress fibers after cytochalasin D-induced actin depolymerization and washout was less effective in dynamin 2-K562E-expressing cells than that in dynamin 2-WT. Bis-T-23, a dynamin self-assembly enhancer, was unable to rescue the decreased focal adhesion numbers and reduced stress fiber density induced by dynamin 2-K562E expression. These results suggest that the low affinity of the K562E mutant for lipid membranes, and atypical self-assembling properties, lead to actin disorganization in HPCs. Moreover, lipid-binding and self-assembly of dynamin 2 along actin filaments are required for podocyte morphology and functions. Finally, dynamin 2-mediated interactions between actin and membranes are critical for actin bundle formation in HPCs.

DOI： 10.3389/fcell.2022.884509

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Microtubule assembly by soluble tau impairs vesicle endocytosis and excitatory neurotransmission via dynamin sequestration in Alzheimer's disease mice synapse model Reviewed International journal

Tetsuya Hori, Kohgaku Eguchi, Han-Ying Wang, Tomohiro Miyasaka, Laurent Guillaud, Zacharie Taoufiq, Satyajit Mahapatra, Hiroshi Yamada, Kohji Takei, Tomoyuki Takahashi

eLife 11 2022.4

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Elevation of soluble wild-type (WT) tau occurs in synaptic compartments in Alzheimer's disease. We addressed whether tau elevation affects synaptic transmission at the calyx of Held in slices from mice brainstem. Whole-cell loading of WT human tau (h-tau) in presynaptic terminals at 10-20 µM caused microtubule (MT) assembly and activity-dependent rundown of excitatory neurotransmission. Capacitance measurements revealed that the primary target of WT h-tau is vesicle endocytosis. Blocking MT assembly using nocodazole prevented tau-induced impairments of endocytosis and neurotransmission. Immunofluorescence imaging analyses revealed that MT assembly by WT h-tau loading was associated with an increased MT-bound fraction of the endocytic protein dynamin. A synthetic dodecapeptide corresponding to dynamin-1-pleckstrin-homology domain inhibited MT-dynamin interaction and rescued tau-induced impairments of endocytosis and neurotransmission. We conclude that elevation of presynaptic WT tau induces de novo assembly of MTs, thereby sequestering free dynamins. As a result, endocytosis and subsequent vesicle replenishment are impaired, causing activity-dependent rundown of neurotransmission.

DOI： 10.7554/elife.73542

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Other Link： https://cdn.elifesciences.org/articles/73542/elife-73542-v1.xml
Imaging‐based evaluation of pathogenicity by novel DNM2 variants associated with centronuclear myopathy Reviewed

Kenshiro Fujise, Mariko Okubo, Tadashi Abe, Hiroshi Yamada, Kohji Takei, Ichizo Nishino, Tetsuya Takeda, Satoru Noguchi

Human Mutation 43 ( 2 ) 169 - 179 2022

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Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

DOI： 10.1002/humu.24307

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Dynamin 2 and BAR domain protein pacsin 2 cooperatively regulate formation and maturation of podosomes Reviewed International journal

Jianzhen Li, Kenshiro Fujise, Haymar Wint, Yosuke Senju, Shiro Suetsugu, Hiroshi Yamada, Kohji Takei, Tetsuya Takeda

Biochemical and Biophysical Research Communications 571 145 - 151 2021.9

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Podosomes are actin-rich adhesion structures formed in a variety of cell types, such as monocytic cells or cancer cells, to facilitate attachment to and degradation of the extracellular matrix (ECM). Previous studies showed that dynamin 2, a large GTPase involved in membrane remodeling and actin organization, is required for podosome function. However, precise roles of dynamin 2 at the podosomes remain to be elucidated. In this study, we identified a BAR (Bin-Amphiphysin-Rvs167) domain protein pacsin 2 as a functional partner of dynamin 2 at podosomes. Dynamin 2 and pacsin 2 interact and co-localize to podosomes in Src-transformed NIH 3T3 (NIH-Src) cells. RNAi of either dynamin 2 or pacsin 2 in NIH-Src cells inhibited podosome formation and maturation, suggesting essential and related roles at podosomes. Consistently, RNAi of pacsin 2 prevented dynamin 2 localization to podosomes, and reciprocal RNAi of dynamin 2 prevented pacsin 2 localization to podosomes. Taking these results together, we conclude that dynamin 2 and pacsin 2 co-operatively regulate organization of podosomes in NIH-Src cells.

DOI： 10.1016/j.bbrc.2021.07.041

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JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes Reviewed

Ayuko Sakane, Taka-aki Yano, Takayuki Uchihashi, Kazuki Horikawa, Yusuke Hara, Issei Imoto, Shusaku Kurisu, Hiroshi Yamada, Kohji Takei, Takuya Sasaki

Communications Biology 4 ( 1 ) 2021.5

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Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

<title>Abstract</title>Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation via activated Rab8A. In association with Rab8A, JRAB/MICAL-L2 adopts its closed form, which functions in the tubulation of recycling endosomes. Moreover, JRAB/MICAL-L2 induces liquid–liquid phase separation, initiating the formation of tubular recycling endosomes upon overexpression. Between its N-terminal and C-terminal globular domains, JRAB/MICAL-L2 contains an intrinsically disordered region, which contributes to the formation of JRAB/MICAL-L2 condensates. Based on our findings, we propose that JRAB/MICAL-L2 plays two sequential roles in the biogenesis of tubular recycling endosomes: first, JRAB/MICAL-L2 organizes phase separation, and then the closed form of JRAB/MICAL-L2 formed by interaction with Rab8A promotes endosomal tubulation.

DOI： 10.1038/s42003-021-02080-7

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Other Link： http://www.nature.com/articles/s42003-021-02080-7
Mutant BIN1-Dynamin 2 complexes dysregulate membrane remodeling in the pathogenesis of centronuclear myopathy. Reviewed International journal

Kenshiro Fujise, Mariko Okubo, Tadashi Abe, Hiroshi Yamada, Ichizo Nishino, Satoru Noguchi, Kohji Takei, Tetsuya Takeda

The Journal of Biological Chemistry 2020.11

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Membrane remodeling is required for dynamic cellular processes such as cell division, polarization and motility. BAR domain proteins and dynamins are key molecules in membrane remodeling that work together for membrane deformation and fission. In striated muscles, sarcolemmal invaginations termed T-tubules are required for excitation-contraction coupling. BIN1 and DNM2, which encode a BAR domain protein BIN1 and dynamin 2, respectively, have been reported to be causative genes of centronuclear myopathy (CNM), a hereditary degenerative disease of skeletal muscle, and deformation of T-tubules is often observed in the CNM patients. However, it remains unclear how BIN1 and dynamin 2 are implicated in T-tubule biogenesis, and how mutations in these molecules cause CNM to develop.Here, using an in cellulo reconstitution assay, we demonstrate that dynamin 2 is required for stabilization of membranous structures equivalent to T-tubules. GTPase activity of wild type dynamin 2 is suppressed through interaction with BIN1, whereas that of the disease-associated mutant dynamin 2 remains active due to lack of the BIN1-mediated regulation thus causing aberrant membrane remodeling. Finally, we show that in cellulo aberrant membrane remodeling by mutant dynamin 2 variants is correlated with their enhanced membrane fission activities, and the results can explain severity of the symptoms in patients. Thus, this study provides molecular insights into dysregulated membrane remodeling triggering the pathogenesis of DNM2-related centronuclear myopathy.

DOI： 10.1074/jbc.RA120.015184

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Initial phospholipid-dependent Irgb6 targeting to Toxoplasma gondii vacuoles mediates host defense. Reviewed International journal

Youngae Lee, Hiroshi Yamada, Ariel Pradipta, Ji Su Ma, Masaaki Okamoto, Hikaru Nagaoka, Eizo Takashima, Daron M Standley, Miwa Sasai, Kohji Takei, Masahiro Yamamoto

Life science alliance 3 ( 1 ) 2020.1

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Toxoplasma gondii is an obligate intracellular protozoan parasite capable of infecting warm-blooded animals by ingestion. The organism enters host cells and resides in the cytoplasm in a membrane-bound parasitophorous vacuole (PV). Inducing an interferon response enables IFN-γ-inducible immunity-related GTPase (IRG protein) to accumulate on the PV and to restrict parasite growth. However, little is known about the mechanisms by which IRG proteins recognize and destroy T. gondii PV. We characterized the role of IRG protein Irgb6 in the cell-autonomous response against T. gondii, which involves vacuole ubiquitination and breakdown. We show that Irgb6 is capable of binding a specific phospholipid on the PV membrane. Furthermore, the absence of Irgb6 causes reduced targeting of other effector IRG proteins to the PV. This suggests that Irgb6 has a role as a pioneer in the process by which multiple IRG proteins access the PV. Irgb6-deficient mice are highly susceptible to infection by a strain of T. gondii avirulent in wild-type mice.

DOI： 10.26508/lsa.201900549

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Internalization of AMPA-type Glutamate Receptor in the MIN6 Pancreatic β-cell Line Reviewed International journal

The Mon La, Hiroshi Yamada, Sayaka Seiriki, Shun-AI Li, Kenshiro Fujise, Natsuho Katsumi, Tadashi Abe, Masami Watanabe, Kohji Takei

Cell Structure and Function 45 ( 2 ) 121 - 130 2020

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Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Cell Biology

DOI： 10.1247/csf.20020

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ATP6AP2 variant impairs CNS development and neuronal survival to cause fulminant neurodegeneration. Reviewed International coauthorship International journal

Takuo Hirose, Alfredo Cabrera-Socorro, David Chitayat, Thomas Lemonnier, Olivier Féraud, Carmen Cifuentes-Diaz, Nicolas Gervasi, Cedric Mombereau, Tanay Ghosh, Loredana Stoica, Jeanne d'Arc Al Bacha, Hiroshi Yamada, Marcel A Lauterbach, Marc Guillon, Kiriko Kaneko, Joy W Norris, Komudi Siriwardena, Susan Blasér, Jérémie Teillon, Roberto Mendoza-Londono, Marion Russeau, Julien Hadoux, Sadayoshi Ito, Pierre Corvol, Maria G Matheus, Kenton R Holden, Kohji Takei, Valentina Emiliani, Annelise Bennaceur-Griscelli, Charles E Schwartz, Genevieve Nguyen, Matthias Groszer

The Journal of Clinical Investigation 129 ( 5 ) 2145 - 2162 2019.4

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Vacuolar H+-ATPase-dependent (V-ATPase-dependent) functions are critical for neural proteostasis and are involved in neurodegeneration and brain tumorigenesis. We identified a patient with fulminant neurodegeneration of the developing brain carrying a de novo splice site variant in ATP6AP2 encoding an accessory protein of the V-ATPase. Functional studies of induced pluripotent stem cell-derived (iPSC-derived) neurons from this patient revealed reduced spontaneous activity and severe deficiency in lysosomal acidification and protein degradation leading to neuronal cell death. These deficiencies could be rescued by expression of full-length ATP6AP2. Conditional deletion of Atp6ap2 in developing mouse brain impaired V-ATPase-dependent functions, causing impaired neural stem cell self-renewal, premature neuronal differentiation, and apoptosis resulting in degeneration of nearly the entire cortex. In vitro studies revealed that ATP6AP2 deficiency decreases V-ATPase membrane assembly and increases endosomal-lysosomal fusion. We conclude that ATP6AP2 is a key mediator of V-ATPase-dependent signaling and protein degradation in the developing human central nervous system.

DOI： 10.1172/JCI79990

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Phosphorylation of cortactin by cyclin-dependent kinase 5 modulates actin bundling by the dynamin 1-cortactin ring-like complex and formation of filopodia and lamellipodia in NG108-15 glioma-derived cells. Reviewed International journal

Tadashi Abe, The Mon La, Yuuzi Miyagaki, Eri Oya, Fan-Yan Wei, Kento Sumida, Kenshiro Fujise, Tetsuya Takeda, Kazuhito Tomizawa, Kohji Takei, Hiroshi Yamada

International Journal of Oncology 54 ( 2 ) 550 - 558 2019.2

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Dynamin copolymerizes with cortactin to form a ring‑like complex that bundles and stabilizes actin filaments. Actin bundle formation is crucial for generation of filopodia and lamellipodia, which guide migration, invasion, and metastasis of cancer cells. However, it is unknown how the dynamin‑cortactin complex regulates actin bundle formation. The present study investigated phosphorylation of cortactin by cyclin‑dependent kinase 5 (CDK5) and its effect on actin bundle formation by the dynamin‑cortactin complex. CDK5 directly phosphorylated cortactin at T145/T219 in vitro. Phosphomimetic mutants in which one or both of these threonine residues was substituted by aspartate were used. The three phosphomimetic mutants (T145D, T219D and T145DT219D) had a decreased affinity for F‑actin. Furthermore, electron microscopy demonstrated that these phosphomimetic mutants could not form a ring‑like complex with dynamin 1. Consistently, the dynamin 1‑phosphomimetic cortactin complexes exhibited decreased actin‑bundling activity. Expression of the phosphomimetic mutants resulted in not only aberrant lamellipodia and short filopodia but also cell migration in NG108‑15 glioma‑derived cells. These results indicate that phosphorylation of cortactin by CDK5 regulates formation of lamellipodia and filopodia by modulating dynamin 1/cortactin‑dependent actin bundling. Taken together, these findings suggest that CDK5 is a potential molecular target for anticancer therapy.

DOI： 10.3892/ijo.2018.4663

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A Novel Membrane Fission Mechanism by Dynamin Complex: Clusterase Model

TAKEI Kohji, YAMADA Hiroshi, TAKEDA Tetsuya

Seibutsu Butsuri 59 ( 5 ) 255 - 261 2019

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Language：Japanese Publisher：The Biophysical Society of Japan General Incorporated Association

Dynamin GTPase, an essential endocytotic protein, helically polymerizes at the neck of endocytic pits, and mechanically sever the membrane upon GTP hydrolysis. However, it is not known exactly how the dynamin disconnect the membrane. To clarify the mechanisms we analyzed structural changes of dynamin complexes during membrane fission using electron microscopy and high-speed atomic force microscopy (HS-AFM). Surprisingly, the dynamin ring complexes were clustered upon GTP hydrolysis and membrane constriction occurred at uncoated regions between the clusters, suggesting a novel mode of action of dynamin. In this commentary, we illustrate dynamin’s membrane fission models proposed thus far, and our novel “clusterase” model.

DOI： 10.2142/biophys.59.255

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Other Link： http://id.ndl.go.jp/bib/030043645
Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis Reviewed

Tetsuya Takeda, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei

eLife 7 2018.1

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Language：English Publishing type：Research paper (scientific journal) Publisher：eLife Sciences Publications Ltd

DOI： 10.7554/eLife.30246

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Dynamin2 GTPase contributes to invadopodia formation in invasive bladder cancer cells Reviewed

Yubai Zhang, Maya Nolan, Hiroshi Yamada, Masami Watanabe, Yasutomo Nasu, Kohji Takei, Tetsuya Takeda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 480 ( 3 ) 409 - 414 2016.11

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DOI： 10.1016/j.bbrc.2016.10.063

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Actin bundling by dynamin 2 and cortactin is implicated in cell migration by stabilizing filopodia in human non-small cell lung carcinoma cells Reviewed

Hiroshi Yamada, Tetsuya Takeda, Hiroyuki Michiue, Tadashi Abe, Kohji Takei

International Journal of Oncology 49 ( 3 ) 877 - 886 2016.9

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.3892/ijo.2016.3592

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Inhibition of SGLT2 alleviates diabetic nephropathy by suppressing high glucose-induced oxidative stress in type 1 diabetic mice Reviewed

Takashi Hatanaka, Daisuke Ogawa, Hiromi Tachibana, Jun Eguchi, Tatsuyuki Inoue, Hiroshi Yamada, Kohji Takei, Hirofumi Makino, Jun Wada

Pharmacology Research and Perspectives 4 ( 4 ) e00239 2016.8

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DOI： 10.1002/prp2.239

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Expression of a dynamin 2 mutant associated with Charcot-Marie-Tooth disease leads to aberrant actin dynamics and lamellipodia formation Reviewed

Hiroshi Yamada, Kinue Kobayashi, Yubai Zhang, Tetsuya Takeda, Kohji Takei

Neuroscience Letters 628 179 - 185 2016.8

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DOI： 10.1016/j.neulet.2016.06.030

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Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization. Reviewed International journal

Keiichiro Hayashi, Hiroyuki Michiue, Hiroshi Yamada, Katsuyoshi Takata, Hiroki Nakayama, Fan-Yan Wei, Atsushi Fujimura, Hiroshi Tazawa, Akira Asai, Naohisa Ogo, Hiroyuki Miyachi, Tei-ichi Nishiki, Kazuhito Tomizawa, Kohji Takei, Hideki Matsui

Scientific reports 6 23372 - 23372 2016.3

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DOI： 10.1038/srep23372

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Possible role of cortactin phosphorylation by protein kinase Cα in actin-bundle formation at growth cone Reviewed International journal

Hiroshi Yamada, Tatsuya Kikuchi, Toshio Masumoto, Fan-Yan Wei, Tadashi Abe, Tetsuya Takeda, Teiichi Nishiki, Kazuhito Tomizawa, Masami Watanabe, Hideki Matsui, Kohji Takei

Biology of the Cell 107 ( 9 ) 319 - 330 2015.9

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DOI： 10.1111/boc.201500032

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Metallothionein deficiency exacerbates diabetic nephropathy in streptozotocin-induced diabetic mice Reviewed

Hiromi Tachibana, Daisuke Ogawa, Norio Sogawa, Masato Asanuma, Ikuko Miyazaki, Naoto Terami, Takashi Hatanaka, Chikage Sato Horiguchi, Atsuko Nakatsuka, Jun Eguchi, Jun Wada, Hiroshi Yamada, Kohji Takei, Hirofumi Makino

American Journal of Physiology - Renal Physiology 306 ( 1 ) F105 - F115 2014.1

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DOI： 10.1152/ajprenal.00034.2013

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N′-[4-(dipropylamino)benzylidene]-2-hydroxybenzohydrazide is a dynamin GTPase inhibitor that suppresses cancer cell migration and invasion by inhibiting actin polymerization Reviewed

Hiroshi Yamada, Tadashi Abe, Shun-Ai Li, Shota Tago, Peng Huang, Masami Watanabe, Satoru Ikeda, Naohisa Ogo, Akira Asai, Kohji Takei

Biochemical and Biophysical Research Communications 443 ( 2 ) 511 - 517 2014.1

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DOI： 10.1016/j.bbrc.2013.11.118

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Long-term treatment with the sodium glucose cotransporter 2 inhibitor, dapagliflozin, ameliorates glucose homeostasis and diabetic nephropathy in db/db mice. Reviewed International journal

Naoto Terami, Daisuke Ogawa, Hiromi Tachibana, Takashi Hatanaka, Jun Wada, Atsuko Nakatsuka, Jun Eguchi, Chikage Sato Horiguchi, Naoko Nishii, Hiroshi Yamada, Kohji Takei, Hirofumi Makino

PloS one 9 ( 6 ) e100777 2014

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DOI： 10.1371/journal.pone.0100777

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Stabilization of Actin Bundles by a Dynamin 1/Cortactin Ring Complex Is Necessary for Growth Cone Filopodia Reviewed

H. Yamada, T. Abe, A. Satoh, N. Okazaki, S. Tago, K. Kobayashi, Y. Yoshida, Y. Oda, M. Watanabe, K. Tomizawa, H. Matsui, K. Takei

Journal of Neuroscience 33 ( 10 ) 4514 - 4526 2013.3

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DOI： 10.1523/jneurosci.2762-12.2013

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Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line. Reviewed International journal

Peng Huang, Masami Watanabe, Haruki Kaku, Hideo Ueki, Hirofumi Noguchi, Morito Sugimoto, Takeshi Hirata, Hiroshi Yamada, Kohji Takei, Shaobo Zheng, Kai Xu, Yasutomo Nasu, Yasuyuki Fujii, Chunxiao Liu, Hiromi Kumon

Molecular and clinical oncology 1 ( 1 ) 180 - 184 2013.1

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Cancer stem cells (CSCs) are thought to be crucial for understanding the biological roots of cancer, and are of increasing importance as a target for new anticancer agents. According to an expression analysis of the cell surface antigens of various types of cancer, CD133 is considered to be a potential marker of cancer stemness. In this study, a human urinary bladder cancer cell line (J82) was used to analyze the cancer stem cell-like characteristics of CD133+ bladder cancer cells in vitro and in vivo. The CD133 expression in the J82 cells was examined and the cells were immunomagnetically categorized into positive and negative subsets. The CD133- and CD133+ subsets were phenotypically divergent with regard to the cell growth pattern, while CD133+ cells tended to colonize during their growth. In CD133+ cells, the pluripotent stem cell factors Oct-4 and Sox-2 were upregulated, and a statistically significant proliferation increase was observed when compared to CD133- cells. The CD133+ subpopulation was more tolerant to the chemotherapeutic agent cisplatin, and Bacillus Calmette-Guérin (BCG), an agent instilled intravesically to treat bladder cancer. In addition, CD133+ J82 cells were more resistant to radiation treatment when compared to CD133- cells. The in vivo tumorigenesis of the CD133- and CD133+ subsets of J82 cancer cells was also examined by subcutaneously injecting them into nude mice. The tumor growth was more aggressive in the CD133+ subpopulation, showing a significant difference in the tumorigenic potential in these subsets. In conclusion, J82 human bladder cancer cells include CD133- and CD133+ subpopulations, while the CD133 molecule is a potential marker of the potential malignancy of human bladder cancer. In the present study, the CD133+ subpopulation was herein demonstrated to have certain characteristics consistent with those of cancer stem cells.

DOI： 10.3892/mco.2012.29

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REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer. Reviewed International journal

Takeshi Hirata, Masami Watanabe, Haruki Kaku, Yasuyuki Kobayashi, Hiroshi Yamada, Masakiyo Sakaguchi, Kohji Takei, Nam-Ho Huh, Yasutomo Nasu, Hiromi Kumon

International journal of oncology 41 ( 2 ) 559 - 64 2012.8

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DOI： 10.3892/ijo.2012.1503

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A novel gene expression system for detecting viable bladder cancer cells Reviewed

Hideo Ueki, Masami Watanabe, Haruki Kaku, Peng Huang, Shun-Ai Li, Kazuhiko Ochiai, Takeshi Hirata, Hirofumi Noguchi, Hiroshi Yamada, Kohji Takei, Yasutomo Nasu, Yuji Kashiwakura, Hiromi Kumon

INTERNATIONAL JOURNAL OF ONCOLOGY 41 ( 1 ) 135 - 140 2012.7

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DOI： 10.3892/ijo.2012.1417

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Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development Reviewed

Kai Zhang, Masami Watanabe, Yuji Kashiwakura, Shun-Al Li, Kohei Edamura, Peng Huang, Ken Yamaguchi, Yasutomo Nasu, Yasuyuki Kobayashi, Masakiyo Sakaguchi, Kazuhiko Ochiai, Hiroshi Yamada, Kohji Takei, Hideo Ueki, Nam-Ho Huh, Ming Li, Haruki Kaku, Yanqun Na, Hiromi Kumon

INTERNATIONAL JOURNAL OF ONCOLOGY 37 ( 6 ) 1495 - 1501 2010.12

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DOI： 10.3892/ijo_00000802

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Use of liposomes to study vesicular transport. Reviewed International journal

Kohji Takei, Hiroshi Yamada, Tadashi Abe

Methods in molecular biology (Clifton, N.J.) 606 531 - 42 2010

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Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

DOI： 10.1007/978-1-60761-447-0_36

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Dynamic Interaction of Amphiphysin with N-WASP Regulates Actin Assembly Reviewed

Hiroshi Yamada, Sergi Padilla-Parra, Sun-Joo Park, Toshiki Itoh, Mathilde Chaineau, Ilaria Monaldi, Ottavio Cremona, Fabio Benfenati, Pietro De Camilli, Maïté Coppey-Moisan, Marc Tramier, Thierry Galli, Kohji Takei

Journal of Biological Chemistry 284 ( 49 ) 34244 - 34256 2009.12

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DOI： 10.1074/jbc.m109.064204

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Dynasore, a dynamin inhibitor, suppresses lamellipodia formation and cancer cell invasion by destabilizing actin filaments Reviewed

Hiroshi Yamada, Tadashi Abe, Shun-Ai Li, Yuki Masuoka, Mihoko Isoda, Masami Watanabe, Yasutomo Nasu, Hiromi Kumon, Akira Asai, Kohji Takei

Biochemical and Biophysical Research Communications 390 ( 4 ) 1142 - 1148 2009.12

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DOI： 10.1016/j.bbrc.2009.10.105

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Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells Reviewed

Atsushi Otsuka, Tadashi Abe, Masami Watanabe, Hitoshi Yagisawa, Kohji Takei, Hiroshi Yamada

Biochemical and Biophysical Research Communications 378 ( 3 ) 478 - 482 2009.1

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DOI： 10.1016/j.bbrc.2008.11.066

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Dynamin 2 cooperates with amphiphysin 1 in phagocytosis in sertoli cells. Reviewed

Akira Nakanishi, Tadashi Abe, Masami Watanabe, Kohji Takei, Hiroshi Yamada

Acta medica Okayama 62 ( 6 ) 385 - 91 2008.12

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Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound in vitro. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested.

DOI： 10.18926/AMO/30954

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Amphiphysin 1 Is Important for Actin Polymerization during Phagocytosis Reviewed

Hiroshi Yamada, Emiko Ohashi, Tadashi Abe, Norihiro Kusumi, Shun-AI Li, Yumi Yoshida, Masami Watanabe, Kazuhito Tomizawa, Yuji Kashiwakura, Hiromi Kumon, Hideki Matsui, Kohji Takei

Molecular Biology of the Cell 18 ( 11 ) 4669 - 4680 2007.11

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DOI： 10.1091/mbc.e07-04-0296

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Major Cdk5-dependent phosphorylation sites of amphiphysin 1 are implicated in the regulation of the membrane binding and endocytosis. Reviewed International journal

Shuang Liang, Fan-Yan Wei, Yu-Mei Wu, Kenji Tanabe, Tadashi Abe, Yoshiya Oda, Yumi Yoshida, Hiroshi Yamada, Hideki Matsui, Kazuhito Tomizawa, Kohji Takei

Journal of neurochemistry 102 ( 5 ) 1466 - 1476 2007.9

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DOI： 10.1111/j.1471-4159.2007.04507.x

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Identification of Vanabin-interacting protein 1 (VIP1) from blood cells of the vanadium-rich ascidian Ascidia sydneiensis samea Reviewed

Tatsuya Ueki, Koki Shintaku, Yuki Yonekawa, Nariaki Takatsu, Hiroshi Yamada, Toshiyuki Hamada, Hiroshi Hirota, Hitoshi Michibata

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1770 ( 6 ) 951 - 957 2007.6

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DOI： 10.1016/j.bbagen.2007.02.003

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Implication of amphiphysin 1 and dynamin 2 in tubulobulbar complex formation and spermatid release. Reviewed

Norihiro Kusumi, Masami Watanabe, Hiroshi Yamada, Shun-Ai Li, Yuji Kashiwakura, Takashi Matsukawa, Atsushi Nagai, Yasutomo Nasu, Hiromi Kumon, Kohji Takei

Cell structure and function 32 ( 2 ) 101 - 13 2007

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Localization of Vanabins, vanadium-binding proteins, in the blood cells of the vanadium-rich ascidian, Ascidia sydneiensis samea Reviewed

Nobuo Yamaguchi, Yuko Amakawa, Hiroshi Yamada, Tatsuya Ueki, Hitoshi Michibata

ZOOLOGICAL SCIENCE 23 ( 10 ) 909 - 915 2006.10

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DOI： 10.2108/zsj.23.909

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Immunohistochemical localization of Klotho protein in brain, kidney, and reproductive organs of mice. Reviewed

Shun-Ai Li, Masami Watanabe, Hiroshi Yamada, Atsushi Nagai, Masahiro Kinuta, Kohji Takei

Cell structure and function 29 ( 4 ) 91 - 9 2004.12

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ジエチルニトロソアミン投与アカタラセミアマウスにおけるビタミンEの効果 Reviewed

益岡典芳, 汪達紘, 太田潤, 山田輝夫, 石橋直樹, 森將晏, 池田己喜子, 山田浩司, 吉良尚平

ビタミンE研究の進歩XI（ビタミンE研究会編） 40 - 44 2004.12

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Dynamin-2 regulates oxidized low-density lipoprotein-induced apoptosis of vascular smooth muscle cell Reviewed

Y Kashiwakura, M Watanabe, N Kusumi, K Sumiyoshi, Y Nasu, H Yamada, T Sawamura, H Kumon, K Takei, H Daida

CIRCULATION 110 ( 21 ) 3329 - 3334 2004.11

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DOI： 10.1161/01.CIR.0000147828.86593.85

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Formation of S-[2-carboxy-1-(1H-imidazol-4-yl) ethyl]glutathione, a new metabolite of L-histidine, from cis-urocanic acid and glutathione by the action of glutathione S-transferase. Reviewed International journal

Masahiro Kinuta, Keiko Kinuta, Hiroshi Yamada, Tadashi Abe, Yumi Yoshida, Kenta Araki, Shun-Ai Li, Atsushi Otsuka, Akira Nakanishi, Yoshinori Moriyama, Kohji Takei

Electrophoresis 24 ( 18 ) 3212 - 8 2003.9

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DOI： 10.1002/elps.200305582

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Secretory granule-mediated co-secretion of L-glutamate and glucagon triggers glutamatergic signal transmission in islets of Langerhans. Reviewed International journal

Mitsuko Hayashi, Hiroshi Yamada, Shunsuke Uehara, Riyo Morimoto, Akiko Muroyama, Shouki Yatsushiro, Jun Takeda, Akitsugu Yamamoto, Yoshinori Moriyama

The Journal of biological chemistry 278 ( 3 ) 1966 - 74 2003.1

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DOI： 10.1074/jbc.M206758200

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Distribution of dynamins in testis and their possible relation to spermatogenesis. Reviewed International journal

Akihiro Kamitani, Hiroshi Yamada, Masahiro Kinuta, Masami Watanabe, Shun-Ai Li, Takashi Matsukawa, Mark McNiven, Hiromi Kumon, Kohji Takei

Biochemical and biophysical research communications 294 ( 2 ) 261 - 7 2002.6

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DOI： 10.1016/S0006-291X(02)00470-9

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Norepinephrine triggers Ca2+-dependent exocytosis of 5-hydroxytryptamine from rat pinealocytes in culture. Reviewed International journal

Hiroshi Yamada, Mitsuko Hayashi, Shunsuke Uehara, Mika Kinoshita, Akiko Muroyama, Masami Watanabe, Koji Takei, Yoshinori Moriyama

Journal of neurochemistry 81 ( 3 ) 533 - 40 2002.5

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Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol. Reviewed International journal

Masahiro Kinuta, Hiroshi Yamada, Tadashi Abe, Masami Watanabe, Shun-Ai Li, Akihiro Kamitani, Tatsuji Yasuda, Takashi Matsukawa, Hiromi Kumon, Kohji Takei

Proceedings of the National Academy of Sciences of the United States of America 99 ( 5 ) 2842 - 7 2002.3

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DOI： 10.1073/pnas.261715599

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Identification and Characterization of a Synaptojanin 2 Splice Isoform Predominantly Expressed in Nerve Terminals Reviewed

Yasuo Nemoto, Markus R. Wenk, Masami Watanabe, Laurie Daniell, Tomoe Murakami, Niels Ringstad, Hiroshi Yamada, Kohji Takei, Pietro De Camilli

Journal of Biological Chemistry 276 ( 44 ) 41133 - 41142 2001.11

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DOI： 10.1074/jbc.M106404200

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Determination of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione, a novel metabolite of L-histidine, in tissue extracts from sunlight-irradiated rat by capillary electrophoresis Reviewed

M Kinuta, J Ohta, H Yamada, K Kinuta, T Abe, SA Li, A Otsuka, A Nakanishi, K Takei

ELECTROPHORESIS 22 ( 16 ) 3365 - 3370 2001.10

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D-aspartate is stored in secretory granules and released through a Ca2+-dependent pathway in a subset of rat pheochromocytoma PC12 cells Reviewed

S Nakatsuka, M Hayashi, A Muroyama, M Otsuka, S Kozaki, H Yamada, Y Moriyama

JOURNAL OF BIOLOGICAL CHEMISTRY 276 ( 28 ) 26589 - 26596 2001.7

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Ca²⁺-dependent exocytosis of L-glutamate by αTC6, clonal mouse pancreatic α-cells Reviewed

Hiroshi Yamada, Masato Otsuka, Mitsuko Hayashi, Shuuichi Nakatsuka, Kazuyuki Hamaguchi, Akitsugu Yamamoto, Yoshinori Moriyama

Diabetes 50 ( 5 ) 1012 - 1020 2001

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DOI： 10.2337/diabetes.50.5.1012

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Vacuolar H⁺-ATPase localized in plasma membranes of malaria parasite cells, Plasmodium falciparum, is involved in regional acidification of parasitized erythrocytes Reviewed

Mitsuko Hayashi, Hiroshi Yamada, Toshihide Mitamura, Toshihiro Horii, Akitsugu Yamamoto, Yoshinori Moriyama

Journal of Biological Chemistry 275 ( 44 ) 34353 - 34358 2000.11

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DOI： 10.1074/jbc.M003323200

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Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells Reviewed

M Takeda, M Haga, H Yamada, M Kinoshita, M Otsuka, S Tsuboi, Y Moriyama

NEUROSCIENCE LETTERS 294 ( 2 ) 97 - 100 2000.11

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Ionotropic glutamate receptors trigger microvesicle-mediated exocytosis of L-glutamate in rat pinealocytes Reviewed

S Yatsushiro, H Yamada, M Hayashi, A Yamamoto, Y Moriyama

JOURNAL OF NEUROCHEMISTRY 75 ( 1 ) 288 - 297 2000.7

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Synaptic-like microvesicles, synaptic vesicle counterparts in endocrine cells, are involved in a novel regulatory mechanism for the synthesis and secretion of hormones Reviewed

Y Moriyama, M Hayashi, H Yamada, S Yatsushiro, S Ishio, A Yamamoto

JOURNAL OF EXPERIMENTAL BIOLOGY 203 ( 1 ) 117 - 125 2000.1

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松果体におけるグルタミン酸を用いた細胞間情報伝達系メラトニン合成の負の制御機構の発見 Reviewed

山田浩司

神経化学 39 ( 1 ) 2000

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Hydroxyindole-O-methyltransferase is another target for L-glutamate-evoked inhibition of melatonin synthesis in rat pinealocytes Reviewed

S Ishio, H Yamada, CM Craft, Y Moriyama

BRAIN RESEARCH 850 ( 1-2 ) 73 - 78 1999.12

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Functional expression of metabotropic glutamate receptor type 5 in rat pinealocytes Reviewed

S Yatsushiro, H Yamada, M Hayashi, S Tsuboi, Y Moriyama

NEUROREPORT 10 ( 7 ) 1599 - 1603 1999.5

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Intrinsic glutaminergic system negatively regulates melatonin synthesis in mammalian pineal gland Reviewed

Y Moriyama, H Yamada, M Hayashi, S Yatsushiro

MELATONIN AFTER FOUR DECADES 460 83 - 90 1999

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Synaptic vesicle protein SV2B, but not SV2A, is predominantly expressed and associated with microvesicles in rat pinealocytes Reviewed

M. Hayashi, S. Yatsushiro, H. Yamada, A. Yamamoto, M. Futai, A. Yamaguchi, Y. Moriyama

Advances in Experimental Medicine and Biology 460 91 - 93 1999

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Acetylcholine triggers L-glutamate exocytosis via nicotinic receptors and inhibits melatonin synthesis in rat pinealocytes Reviewed

H Yamada, A Ogura, S Koizumi, A Yamaguchi, Y Moriyama

JOURNAL OF NEUROSCIENCE 18 ( 13 ) 4946 - 4952 1998.7

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Synaptic vesicle protein SV2B, but not SV2A, is predominantly expressed and associated with microvesicles in rat pinealocytes Reviewed

T Hayashi, A Yamamoto, S Yatsushiro, H Yamada, M Futai, A Yamaguchi, Y Moriyama

JOURNAL OF NEUROCHEMISTRY 71 ( 1 ) 356 - 365 1998.7

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D-aspartate modulates melatonin synthesis in rat pinealocytes Reviewed

S Ishio, H Yamada, M Hayashi, S Yatsushiro, T Noumi, A Yamaguchi, Y Moriyama

NEUROSCIENCE LETTERS 249 ( 2-3 ) 143 - 146 1998.6

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Identification of D-aspartate in rat pheochromocytoma PC12 cells Reviewed

Y Moriyama, H Yamada, M Hayashi, T Oda, A Yamaguchi

NEUROSCIENCE LETTERS 248 ( 1 ) 57 - 60 1998.5

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Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes Reviewed

H Yamada, S Yatsushiro, S Ishio, M Hayashi, T Nishi, A Yamamoto, M Futai, A Yamaguchi, Y Moriyama

JOURNAL OF NEUROSCIENCE 18 ( 6 ) 2056 - 2062 1998.3

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Autonomic regulation of melatonin synthesis through intrinsic glutaminergic systems in mammalian pineal glands. Reviewed

森山芳則, 山田浩司

生化学 70 ( 5 ) 1998

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Functional expression of a GLT-1 type Na+-dependent glutamate transporter in rat pinealocytes Reviewed

H Yamada, S Yatsushiro, A Yamamoto, M Hayashi, T Nishi, M Futai, A Yamaguchi, Y Moriyama

JOURNAL OF NEUROCHEMISTRY 69 ( 4 ) 1491 - 1498 1997.10

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DOI： 10.1046/j.1471-4159.1997.69041491.x

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L-aspartate but not the D form is secreted through microvesicle-mediated exocytosis and is sequestered through Na+-dependent transporter in rat pinealocytes Reviewed

S Yatsushiro, H Yamada, S Kozaki, H Kumon, H Michibata, A Yamamoto, Y Moriyama

JOURNAL OF NEUROCHEMISTRY 69 ( 1 ) 340 - 347 1997.7

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L-Aspartate-evoked inhibition of melatonin production in rat pineal glands Reviewed

H Yamada, A Yamaguchi, Y Moriyama

NEUROSCIENCE LETTERS 228 ( 2 ) 103 - 106 1997.6

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Transmembrane topology of Escherichia coli H+-ATPase (ATP synthase) subunit a Reviewed

Hiroshi Yamada, Yoshinori Moriyama, Masatomo Maeda, Masamitsu Futai

FEBS Letters 390 ( 1 ) 34 - 38 1996.7

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DOI： 10.1016/0014-5793(96)00621-7

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Vesicular monoamine transporter in microvesicles from bovine posterior pituitaries is immunologically similar to but distinct from the chromaffin granule counterpart in its sensitivities to 1-methyl-4-phenylpyridinium and histamine Reviewed

Y Moriyama, H Yamada

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 221 ( 3 ) 790 - 794 1996.4

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Language：English Publishing type：Research paper (scientific journal)

Web of Science

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Role of endocrine cell microvesicles in intercellular chemical transduction. International journal

Y Moriyama, A Yamamoto, H Yamada, Y Tashiro, M Futai

Biological chemistry Hoppe-Seyler 377 ( 3 ) 155 - 65 1996.3

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Microvesicles (MVs) in endocrine cells are morphologically similar to neuronal synaptic vesicles. MVs were shown to contain proteins involved in neurotransmitter storage such as vacuolar H(+)-ATPase and neurotransmitter transporters, and ones in vesicular trafficking such as synaptobrevins and N-ethylmaleimide-sensitive fusion protein. Isolated MVs accumulate cell-specific neurotransmitters in an energy-dependent manner. Upon stimulation, the MVs may fuse with the plasma membrane and secrete the internal neurotransmitters. Thus, endocrine cells possess an MV-mediated secretion system as an intercellular signal transducing system.

PubMed

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Microvesicle-mediated exocytosis of glutamate is a novel paracrine-like chemical transduction mechanism and inhibits melatonin secretion in rat pinealocytes Reviewed

Hiroshi Yamada, Akitsugu Yamamoto, Susumu Yodozawa, Shunji Kozaki, Masami Takahashi, Mitsuhiro Morita, Hitoshi Michibata, Teiichi Furuichi, Katsuhiko Mikoshiba, Yoshinori Moriyama

Journal of Pineal Research 21 ( 3 ) 175 - 191 1996

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Blackwell Publishing Ltd

DOI： 10.1111/j.1600-079X.1996.tb00285.x

Scopus

PubMed

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The L-type Ca2+ channel is involved in microvesicle-mediated glutamate exocytosis from rat pinealocytes Reviewed

Hiroshi Yamada, Akitsugu Yamamoto, Masami Takahashi, Hitoshi Michibata, Hiromi Kumon, Yoshinori Moriyama

Journal of Pineal Research 21 ( 3 ) 165 - 174 1996

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Blackwell Publishing Ltd

DOI： 10.1111/j.1600-079X.1996.tb00284.x

Scopus

PubMed

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Microvesicles isolated from bovine posterior pituitary accumulate norepinephrine Reviewed

Y. Moriyama, A. Yamamoto, H. Yamada, Y. Tashiro, K. I. Tomochika, M. Takahashi, M. Maeda, M. Futai

Journal of Biological Chemistry 270 ( 19 ) 11424 - 11429 1995

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1074/jbc.270.19.11424

Scopus

PubMed

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Inhibition of neurotransmitter and hormone transport into secretory vesicles by 2-(4-phenylpiperidino)cyclohexanol and 2-bromo-α-ergocryptine: Both compounds act as uncouplers and dissipate the electrochemical gradient of protons Reviewed

Yoshinori Moriyama, Kosuke Amakatsu, Hiroshi Yamada, Mi-Yeon Park, Masamitsu Futai

Archives of Biochemistry and Biophysics 290 ( 1 ) 233 - 238 1991

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/0003-9861(91)90614-O

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PubMed

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MISC

オートファジー Invited

竹居孝二, 山田浩司

岡山医学会雑誌 135 92 - 94 2023.8

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Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)

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Dynamic interaction of amphiphysin with N-WASP regulates actin assembly Invited

山田浩司, PADILLA-PARRA Sergi, 朴宣奏, 伊藤俊樹, CHAINEAU Mathilde, MONALDI Ilaria, CREMONA Ottavio, BENFENATI Fabio, De CAMILLI, COPPEY-MOISAN Maïté, TRAMIER Marc, GALLI Thierry, 竹居孝二

岡山医学会雑誌 123 ( 1 ) 1 - 11 2011

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Authorship：Lead author Language：Japanese

J-GLOBAL

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シナプスの可塑性とグルタミン酸受容体のクラスリン被覆小胞による細胞内への取り込み Invited Reviewed

山田浩司

化学と工業 54 ( 6 ) 2001

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Authorship：Lead author,　Last author,　Corresponding author Language：Japanese

J-GLOBAL

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Presentations

糸球体足細胞（ポドサイト）におけるダイナミンによる微小管制御と形態形成 Invited

山田浩司

第９４回日本生化学会大会 2021.11.3

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Event date： 2021.11.3 - 2021.11.5

Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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ダイナミンによる細胞骨格制御 Invited

山田浩司

愛媛大学応用化学セミナー・ミニシンポジウム 2021.7.21

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Event date： 2021.7.21

Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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血液ろ過に重要な新規の腎臓病治療標的タンパク質「ダイナミン１」の発見 Invited

山田浩司

未来のバイオ技術勉強会 2021.4.23 バイオインダストリー協会

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Event date： 2021.4.23

Language：Japanese Presentation type：Oral presentation (invited, special)

Venue：オンライン
シナプトポディン２様タンパク質とアルファーアクチニンとの直接結合は心筋細胞のアクチン線維束形成に寄与する

山田浩司, 大坂紘奈, 辰巳七海, 荒木美羽, 阿部匡史, 貝原恵子, 高橋賢, 高島英造, 内橋貴之, 成瀬恵治, 竹居孝二

第９７回日本生化学会大会 2024.11.7

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Event date： 2024.11.6 - 2024.11.8

Language：Japanese Presentation type：Poster presentation

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骨芽細胞移動を制御するアクチンフィラメント形成におけるダイナミンの役割

守谷拓巳, A SURONG, 山田浩司, 竹居孝二, 上岡寛, 岡村裕彦, 池亀美華

日本解剖学会第７８回中国・四国支部学術集会 2024.10.20

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Event date： 2024.10.19 - 2024.10.20

Language：Japanese Presentation type：Oral presentation (general)

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腎糸球体ポドサイトにおける細胞骨格ダイナミクスの解明

竹居孝二, 阿部匡史, 竹田哲也, 田邊克幸, 山田浩司

第９６回日本生化学会大会 2023.11.1

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Event date： 2023.10.31 - 2023.11.2

Language：Japanese Presentation type：Poster presentation

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TRPV2の細胞膜移行におけるダイナミン1による微小管制御の役割

山田浩司, 阿部匡史, 竹田哲也, 竹居孝二

第９６回日本生化学会大会 2023.11.1

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Event date： 2023.10.31 - 2023.11.2

Language：Japanese Presentation type：Poster presentation

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糸球体ポドサイトにおけるダイナミン１による微小管制御機構 Invited

山田浩司

愛媛大学PRiME 共同研究発表会 2023.9.12

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Event date： 2023.9.12

Presentation type：Oral presentation (invited, special)

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Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells

Haymar Win, Jianzhen Li, Tadashi Abe, Hiroshi Yamada, Takumi Higaki, Yasutomo Nasu, Masami Watanabe, Kohji Takei, Tetsuya Takeda

2023.6.28

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Event date： 2023.6

Language：English Presentation type：Poster presentation

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Irgb6の膜へのリクルートは膜変形を直接誘導する

阿部匡史、山田浩司、長岡ひかる、高島英造、仁田亮、山本雅裕、竹居孝二

第９５回日本生化学会大会 2022.11.11 日本生化学会

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Event date： 2022.11.9 - 2022.11.11

Language：Japanese Presentation type：Poster presentation

Venue：名古屋国際会議場
脂質膜結合活性が低下しているダイナミン２のCMT変異体は、ポドサイトにおけるアクチン線維の配向とアクチンバンドル形成を異常にする

山田浩司, 阿部匡史, 内橋貴之, 成田哲博, 竹田哲也, 竹居孝二

第９５回日本生化学会大会 2022.11.11 日本生化学会

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Event date： 2022.11.9 - 2022.11.11

Language：Japanese Presentation type：Poster presentation

Venue：名古屋国際会議場

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ダイナミンによる微小管制御と糸球体ポドサイトの形態形成

竹居孝二、山田浩司

第７４回日本細胞生物学会大会 2022.6.30 日本細胞生物学会

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Event date： 2022.6.28 - 2022.6.30

Language：Japanese Presentation type：Poster presentation

Venue：タワーホール船堀
ポドサイトにおけるダイナミン２によるアクチン制御にはダイナミン２の適切な自己重合と膜との相互作用が必要である

山田浩司, 内橋貴之, 成田哲博, 竹居孝二

第６回ポドサイト研究会 2022.3.26

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Event date： 2022.3.26

Language：Japanese Presentation type：Oral presentation (general)

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分化ポドサイトにおけるダイナミン１の機能

竹居孝二, 淺沼克彦, 山田浩司

第６回ポドサイト研究会 2022.3.26

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Event date： 2022.3.26

Language：Japanese Presentation type：Oral presentation (general)

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糸球体ポドサイトからのグルタミン酸の放出と開口放出関連タンパクの探索

安岡宏樹, 西井尚子, 原田結加, 宮地孝明, 阿部匡史, 竹田哲也, 和田淳, 竹居孝二, 山田浩司

第６２回日本生化学会中国・四国支部例会 2021.9.11 日本生化学会中国・四国支部

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Event date： 2021.9.10 - 2021.9.11

Language：Japanese Presentation type：Oral presentation (general)

Venue：岡山大学
ダイナミン1は微小管の配向を調節しTRPV2の細胞膜移行を制御する

籔彩夏, 安岡宏樹, 片野坂友紀, 阿部匡史, 竹田哲也, 竹居孝二, 山田浩司

第６２回日本生化学会中国・四国支部例会 2021.9.11 日本生化学会中国・四国支部

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Event date： 2021.9.10 - 2021.9.11

Language：Japanese Presentation type：Oral presentation (general)

Venue：岡山大学
ダイナミン2の自己重合と膜との相互作用は糸球体ポドサイトのアクチン制御に重要である

濱崎英理子, 安岡宏樹, 和木田夏輝, 阿部匡史, 竹田哲也, 竹居孝二, 山田浩司

第６２回日本生化学会中国・四国支部例会 2021.9.11 日本生化学会中国・四国支部

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Event date： 2021.9.10 - 2021.9.11

Language：Japanese Presentation type：Oral presentation (general)

Venue：岡山大学
Amphiphysin1はsynaptopodinと共同してアクチン制御に働く

山田浩司, The Mon La, 阿部匡史, 竹田哲也, 高島英造, 長岡ひかる, 淺沼克彦, 竹居孝二

第４回ポドサイト研究会 2020.3 ポドサイト研究会

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Event date： 2020.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：オンライン
中心核ミオパチー型BIN1およびDNM2変異体による膜リモデリング異常の解析

藤瀬賢志郎, 山田浩司, 竹居孝二, 竹田哲也

日本細胞生物学会大会(Web) 2019

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Event date： 2019

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筋細胞膜リモデリングにおけるメカニカルストレス応答の解析

藤瀬賢志郎, 山田浩司, 竹居孝二, 竹田哲也

日本筋学会学術集会プログラム・抄録集 2019

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Event date： 2019

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腎糸球体ポドサイトにおけるアンフィファイジン1の機能

山田浩司, LA The Mon, 竹田哲也, 阿部匡史, 淺沼克彦, 竹居孝二

日本生化学会大会(Web) 2019

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Event date： 2019

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腎糸球体ポドサイトにおけるダイナミンイソフォームの局在と機能

阿部匡史, LA The Mon, 橘洋美, 竹田哲也, 竹居孝二, 山田浩司

日本生化学会大会(Web) 2019

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Event date： 2019

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メカノエンザイム・ダイナミンGTPaseによるアクチン線維束化機構の解析

山田浩司, 阿部匡史, 竹田哲也, 高島英造, 森田将之, 竹居孝二

日本生物工学会大会講演要旨集 2018.8 (公社)日本生物工学会

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Event date： 2018.8

Language：Japanese

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ダイナミン2のシャルコー・マリー・トゥース病の原因変異とアクチン再構成との相関

隅田健斗, LA The Mon, 和木田夏輝, 森田将之, 高島英造, 竹田哲也, 阿部匡史, 竹居孝二, 山田浩司

日本分子生物学会年会プログラム・要旨集(Web) 2018

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Event date： 2018

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ダイナミン-コルタクチンらせん状複合体の解析:機械的なアクチン線維束形成とアクチン脱重合保護作用

阿部匡史, 山田浩司, 竹田哲也, 内橋貴之, 安藤敏夫, 竹居孝二

日本生化学会大会(Web) 2017

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Event date： 2017

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ダイナミン2のシャルコー・マリー・トゥース病の原因変異は腎ポドサイトのアクチン再構成を阻害する

和木田夏輝, LA The Mon, 隅田健斗, 竹田哲也, 阿部匡史, 竹居孝二, 山田浩司

日本生化学会大会(Web) 2017

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Event date： 2017

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GTP加水分解に共役したダイナミン依存的膜切断機構の高速原子間力顕微鏡解析

竹田哲也, 石黒大輝, 楊恵然, 小財稔矢, 背山佳穂, 熊谷祐介, 山田浩司, 内橋貴之, 安藤敏夫, 竹居孝二

日本細胞生物学会大会(Web) 2017

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Event date： 2017

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ダイナミンによる膜切断機構の高速AFMイメージング解析

竹田哲也, 竹田哲也, 熊谷祐介, 背山佳穂, YANG Huiran, 山田浩司, 山田浩司, 内橋貴之, 内橋貴之, 安藤敏夫, 安藤敏夫, 竹居孝二, 竹居孝二

日本細胞生物学会大会(Web) 2016

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Event date： 2016

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腎糸球体ポドサイトにおけるダイナミンアイソフォームの局在と機能

橘洋美, 竹田哲也, 山田浩司, 小川大輔, 竹居孝二

日本細胞生物学会大会(Web) 2016

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Event date： 2016

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成長円錐におけるPKCαのコルタクチンリン酸化によるアクチン制御の可能性

山田浩司, 菊池達也, 増本年男, 魏范研, 阿部匡史, 竹田哲也, 西木禎一, 富澤一仁, 渡部昌実, 松井秀樹, 竹居孝二

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 2015.12 (公社)日本生化学会

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Event date： 2015.12

Language：English

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腎糸球体ポドサイト分化におけるダイナミンGTPアーゼの役割

橘洋美, 竹田哲也, 山田浩司, 小川大輔, 竹居孝二

日本生化学会大会(Web) 2015

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Event date： 2015

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肺がん細胞株における細胞運動を司るダイナミン2によるアクチン動態制御

阿部匡史, 山田浩司, 竹田哲也, 竹居孝二

日本生化学会大会(Web) 2014

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Event date： 2014

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脳血液関門通過可能なアクチン重合抑制薬による膠芽腫に対する抗浸潤効果の検討

道上宏之, 林桂一郎, 山田浩司, 中山大輝, 黒住和彦, 市川智継, 松下博昭, 西木禎一, 竹居孝二, 富澤一仁, 松井秀樹

日本脳腫瘍学会プログラム・抄録集 2014

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悪性脳腫瘍に対するDrug Repositioning(DR)に基づく新規抗浸潤薬の研究開発

道上宏之, 林桂一郎, 山田浩司, 魏范研, 高田尚良, 藤村篤史, 黒住和彦, 市川智継, 大守伊織, 西木禎一, 竹居孝二, 松井秀樹

日本脳腫瘍学会プログラム・抄録集 2013

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ダイナミンGTPアーゼ/コルタクチン複合体はアクチン線維束を安定化するメカニカルデバイスである

山田浩司, 阿部匡史, 竹居孝二

日本生体エネルギー研究会討論会講演要旨集 2012

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Dynaminを標的にした抗がん剤の開発

岡崎奈奈, 阿部匡史, 有田美香子, 多湖翔太, 永井さや, 池田敏, 小郷尚久, 浅井章良, 山田浩司, 竹居孝二

日本生化学会大会(Web) 2012

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Event date： 2012

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ダイナミン・コルタクチン複合体はアクチン線維を束化し、この束化は成長円錐の糸状仮足形成に重要である

山田浩司, 阿部匡史, 竹居孝二

日本薬学会年会要旨集 2011.3 (公社)日本薬学会

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Event date： 2011.3

Language：English

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PKC phosphorylation of cortactin is implicated in the regulation of actin dynamics

Hiroshi Yamada, Tadashi Abe, Toshio Masumoto, Mari Sei, Tatsuya Kikuchi, Kazuhito Tomizawa, Satoru Ikeda, Hideki Matsui, Kohji Takei

51st Annual meeting, American Society for Cell Biology 2011.12 AMER SOC CELL BIOLOGY

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Event date： 2011

Language：English Presentation type：Poster presentation

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ダイナミン/コルタクチン複合体によるアクチン細胞骨格制御機構

竹居孝二, 山田浩司, 阿部匡

生体膜と薬物の相互作用シンポジウム講演要旨集 2011

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成長円錐糸状仮足形成におけるCortactinのPKCalphaによるリン酸化とアクチン制御の可能性

山田浩司, 勢井麻梨, 阿部匡史, 増本年男, 菊池達也, 富澤一仁, 池田敏, 松井秀樹, 竹居孝二

日本生化学会大会プログラム・講演要旨集 2011 (公社)日本生化学会

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Event date： 2011

Language：Japanese

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ダイナミン1/コルタクチン複合体はアクチン線維束を形成し、成長円錐フィロポディア形成を支持する(Dynamin 1/cortactin complex mechanically bundles actin filaments and supports the formation of growth cone filopodia)

竹居孝二, 阿部匡史, 川田慎浩, 山田浩司

神経化学 2010.8 日本神経化学会

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Language：English

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Dynamic Interaction of Amphiphysin with N-WASP Regulates Actin Assembly

Hiroshi Yamada, Sergi Padilla-Parra, Sun-Joo Park, Toshiki Itoh, Mathilde Chaineau, Ilaria Monaldi, Ottavio Cremona, Fabio Benfenati, Pietro De Camilli, Maite Coppey-Moisan, Marc Tramier, Kohji Takei

33rd Annual Meeting of the Japan Neuroscience Society (Neuro 2010) 2010 ELSEVIER IRELAND LTD

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Event date： 2010

Language：English Presentation type：Poster presentation

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ダイナミン/コルタクチン複合体によるアクチン束化は糸状仮足形成と関連がある(Actin bundling by dynamin/cortactin complex is implicated in filopodia formation)

山田浩司, 阿部匡史, 川田慎浩, 竹居孝二

日本生化学会大会プログラム・講演要旨集 2009.9 (公社)日本生化学会

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Event date： 2009.9

Language：English

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ダイナミン/コルタクチン複合体によるアクチン束形成の新しいメカニズム糸状仮足形成における意義(Novel mechanism of actin bundle formation by dynamin/cortactin complex: its implication in filopodia formation)

山田浩司, 阿部匡史, 吉田祐実, 竹居孝二

日本細胞生物学会大会講演要旨集 2009.5 (一社)日本細胞生物学会

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Event date： 2009.5

Language：English

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ダイナミン1は、コルタクチンとともに、アクチンの動態に働く(Dynamin 1 participates in actin dynamics with cortactin)

信崎哲郎, 吉田祐実, 阿部匡史, 小田吉哉, 富沢一仁, 山田浩司, 竹居孝二

日本細胞生物学会大会講演要旨集 2008.6 (一社)日本細胞生物学会

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Event date： 2008.6

Language：English

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Localization of dynamin2 during mitosis

Nobuhisa Ishida, Shun-Ai Li, Yuuichi Nakamura, Hiroshi Yamada, Toshio Sugahara, Kohji Takei

CELL STRUCTURE AND FUNCTION 2005.6 JAPAN SOC CELL BIOLOGY

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Event date： 2005.6

Language：English Presentation type：Poster presentation

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Analysis of the major cdk5 phosphorylation sites of amphiphysin 1

Shuang Liang, Yumi Yoshida, Kazuhito Tomizawa, Tadashi Abe, Hiroshi Yamada, Hideki Matsui, Kohji Takei

CELL STRUCTURE AND FUNCTION 2005.6 JAPAN SOC CELL BIOLOGY

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Event date： 2005.6

Language：English Presentation type：Poster presentation

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Localization of amphiphysin1 and dynamin2 in tubulobulbar complexes (TBC) formation and sperm release at Sertoli cells

Norihiro Kusumi, Masami Watanabe, Hiroshi Yamada, Shun-Ai Li, Yuji Kashiwakura, Atsushi Nagai, Yasutomo Nasu, Hiromi Kumon, Pietro De Camilli, Kohji Takei

CELL STRUCTURE AND FUNCTION 2005.6 JAPAN SOC CELL BIOLOGY

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Event date： 2005.6

Language：English Presentation type：Poster presentation

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エンドサイトーシス関連タンパク質amphiphysin 1の精巣における機能

久住倫宏, 渡部昌実, 坪井啓, 永井敦, 公文裕巳, 山田浩司, 竹居孝二, 柏倉祐司

日本泌尿器科学会雑誌 2005

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Event date： 2005

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Dynamin 2 is involved in PS-dependent formation of membrane ruffles and phagocytosis in rat sertoli cells

Hiroshi Yamada, Shun-Ai Li, Masami Watanabe, Fan-Yan Wei, Norihiro Kusumi, Kazuhito Tomizawa, Mark McNiven, Hideki Matsui, Hiromi Kumon, Kohji Takei

MOLECULAR BIOLOGY OF THE CELL 2004.12 AMER SOC CELL BIOLOGY

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Event date： 2004.12

Language：English Presentation type：Poster presentation

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Dynamin及びAmphiphysinのリン酸化-脱リン酸化による小胞形成能変化

絹田正裕, 荒木健太, 阿部匡史, 梁爽, 吉田祐美, 山田浩司, 永松知洋, 富澤一仁, 松井秀樹, 保田立二, 竹居孝二

生化学 2004.3 (公社)日本生化学会

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Event date： 2004.3

Language：Japanese

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小胞形成に対するdynamin及びamphiphysinのリン酸化-脱リン酸化の効果

荒木健太, 阿部匡史, 福田功, 富澤一仁, 山田浩司, 須田城, 絹田正裕, 竹居孝二

日本細胞生物学会大会講演要旨集 2003.5 (一社)日本細胞生物学会

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Event date： 2003.5

Language：Japanese

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Expression of endocytic proteins in the testis, and their implication in spermatogenesis.

A Kamitani, M Watanabe, H Iguchi, A Nagai, H Yamada, M Kinuta, K Takei, H Kumon

JOURNAL OF UROLOGY 2002.4 LIPPINCOTT WILLIAMS & WILKINS

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Event date： 2002.4

Language：English

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脳及び精巣におけるKlothoタンパクの発現とKlotho変異マウスのエンドサイトーシス機能タンパクの発現変化

李順愛, 絹田正裕, 紙谷章弘, 紙谷章弘, 山田浩司, 公文裕巳, 竹居孝二

日本細胞生物学会大会講演要旨集 2002

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ラット培養セルトリ細胞におけるDynamin2,3の発現

山田浩司, 紙谷章弘, 渡部昌実, 絹田正裕, 公文裕巳, 竹居孝二

第７５回日本生化学会大会 2002

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Event date： 2002

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ヒトretinoblastoma Y79細胞に発現しているグルタミン酸受容体

木下美香, 武田美智子, 山田浩司, 大塚正人, 坪井誠二, 森山芳則

日本薬学会年会要旨集 2001

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エンドサイトーシスにおけるPtdIns(4,5)P₂の分解

絹田正裕, 山田浩司, 阿部匡史, 李順愛, 渡部昌実, 紙谷章弘, 公文裕巳, 竹居孝二

日本細胞生物学会大会講演要旨集 2001

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Event date： 2001

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ラット培養セルトリ細胞におけるエンドサイトーシス関連蛋白質の発現

紙谷章弘, 山田浩司, 渡部昌実, 絹田正裕, 公文裕巳, 竹居孝二

日本細胞生物学会大会講演要旨集 2001

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ラット松果体細胞からのセロトニンの開口放出

上原俊介, 山田浩司, 林美都子, 木下美香, 渡部昌美, 竹居孝二, 森山芳則

第７４回日本生化学会大会 2001

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Event date： 2001

Language：English Presentation type：Poster presentation

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Ptdlns(4,5)P₂の分解とエンドサイトーシス

山田浩司, 絹田正裕, 阿部匡史, 李順愛, 渡部昌美, 紙谷章弘, 公文裕巳, 竹居孝二

第４４回日本神経化学会 2001

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Event date： 2001

Language：English Presentation type：Poster presentation

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興奮性アミノ酸による内分泌機能制御

森山芳則, 林美都子, 山田浩司, 中塚修一, 木下美香, 室山明子, 広田寿美子, 森本理代, 山本章嗣

日本細胞生物学会大会講演要旨集 2001

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マウスすい臓ランゲルハンス島由来αTC6からのグルタミン酸の開口放出

中塚修一, 山田浩司, 大塚正人, 林美都子, 浜口和之, 山本章嗣, 森山芳則

日本薬学会年会要旨集 2001

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Event date： 2001

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Vesicle formation and membrane lipid kinetics in model synaptic endocytosis using liposomes.

M Kinuta, H Yamada, T Abe, M Watanabe, J Ohta, K Takei

MOLECULAR BIOLOGY OF THE CELL 2000.12 AMER SOC CELL BIOLOGY

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Event date： 2000.12

Language：English

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ヒト・マラリア原虫Plasmodium falciparumにおけるV-ATPaseの局在と機能

林美都子, 山田浩司, 森山芳則, 三田村俊秀, 堀井俊宏, 山本章嗣

日本細胞生物学会大会講演要旨集 2000

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リポソームを用いたエンドサイトーシスのモデル実験と膜脂質の動態

絹田正裕, 阿部匡史, 山田浩司, 太田潤, 渡部昌実, ラタナクルニシャ, 竹居孝二

日本細胞生物学会大会講演要旨集 2000

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細胞膜のダイナミックスエンドサイトーシスにおける細胞膜脂質の動態

竹居孝二, 絹田正裕, 阿部匡史, 山田浩司, 太田潤, 渡部昌実

日本細胞生物学会大会講演要旨集 2000

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Event date： 2000

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グルタミン酸作動性内分泌細胞の発見

森山芳則, 山田浩司, 林美都子, 大塚正人, 八代聖基, 石尾将吾, 中塚修一, 木下美香, 山本章嗣

日本細胞生物学会大会講演要旨集 2000

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Event date： 2000

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ラット松果体におけるイオン型グルタミン酸受容体の発現とその機能

八代聖基, 山田浩司, 林美都子, 坪井誠二, 山本章嗣, 森山芳則

第７２回日本生化学会大会 1999.8 (公社)日本生化学会

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Event date： 1999.8

Language：English Presentation type：Poster presentation

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ラット松果体における代謝調節型グルタミン酸受容体(mGluR5)の機能的発現

山田浩司, 八代聖基, 林美都子, 坪井誠二, 森山芳則

第７２回日本生化学会大会 1999.8 (公社)日本生化学会

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Event date： 1999.8

Language：Japanese Presentation type：Poster presentation

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松果体におけるグルタミン酸によるメラトニンの合成阻害機構

石尾将吾, 八代聖基, 山田浩司, 林美都子, は和めぐみ, 阿蘇寛明, 山本章嗣, 坪井誠二, 森山芳則

生体膜と薬物の相互作用シンポジウム講演要旨集 1999

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Event date： 1999

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Control mechanism of melatonin synthesis through metabolism type glutamate receptor in rat pineal body.

石尾将吾, 八代聖基, 山田浩司, 林美都子, 山口明人, 森山芳則

日本薬学会年会要旨集 1998

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Event date： 1998

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Research on the synthesis control of biological clock hormone melatonin. Discovery of a negative control mechanism through metabolism type glutamate receptor.

山田浩司, 八代聖基, 林美都子, 二井将光, 山口明人, 森山芳則

生体膜と薬物の相互作用シンポジウム講演要旨集 1998

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Event date： 1998

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Molecular cell biology of Plasmodium. Identification and function of acidic compartment.

森山芳則, 林美都子, 山田浩司, 山口明人, 山本章嗣, 三田村俊秀, 堀井俊宏, 金恵淑, 綿矢有佑

生体膜と薬物の相互作用シンポジウム講演要旨集 1998

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Event date： 1998

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Structure and function of the synaptic vesicle and micro-vesicle of endocrine cell.

林美都子, 山本章嗣, 山田浩司, 八代聖基, 山口明人, 二井将光, 森山芳則

生体膜と薬物の相互作用シンポジウム講演要旨集 1998

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Event date： 1998

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Glutamate paracrine secretory mechanism through microvesicle in the pinealocyte.

山田浩司, 山本章嗣, 森山芳則

日本分子生物学会年会プログラム・講演要旨集 1996

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Event date： 1996

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Exocytosis of glutamate from the pineal body parenchymal cells.

山田浩司, 山本章嗣, 田代裕, 道端斉, 森山芳則

日本細胞生物学会大会講演要旨集 1995

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Event date： 1995

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New information transfer system between cells relating to synaptic vesicle-like microvesicle.

森山芳則, 山田浩司, 道端斉, 山本章嗣, 田代裕

日本動物学会大会予稿集 1995

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Event date： 1995

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Acid vesicle existing in Ascidia sydneiensis samea.

林美都子, 浅井純子, 山田浩司, 宇山太郎, 森山芳則, 道端斉

日本動物学会大会予稿集 1995

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Event date： 1995

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Exocytosis of glutamate from pineal body parenchymal cell.

山田浩司, 山本章嗣, 田代裕, 道端斉, 森山芳則

日本動物学会大会予稿集 1995

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Event date： 1995

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New intercellular information transfer syste related to synaptic vesicle-like microvesicle.

森山芳則, 山田浩司, 道端斉, 山本章嗣, 田代裕

日本細胞生物学会大会講演要旨集 1995

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Event date： 1995

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糸球体ポドサイトからのグルタミン酸の放出と開口放出関連タンパクの探索

安岡宏樹, 西井尚子, 原田結加, 宮地孝明, 阿部匡史, 竹田哲也, 和田淳, 竹居孝二, 山田浩司

第６２回日本生化学会中国・四国支部例会 2021.9.11

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ダイナミン1は微小管の配向を調節しTRPV2の細胞膜移行を制御する

籔彩夏, 安岡宏樹, 片野坂友紀, 阿部匡史, 竹田哲也, 竹居孝二, 山田浩司

第６２回日本生化学会中国・四国支部例会 2021.9.11

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ダイナミン2の自己重合と膜との相互作用は糸球体ポドサイトのアクチン制御に重要である

濱崎英理子, 安岡宏樹, 和木田夏輝, 阿部匡史, 竹田哲也, 竹居孝二, 山田浩司

第６２回日本生化学会中国・四国支部例会 2021.9.11

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血液ろ過に重要な新規の腎臓病治療標的タンパク質『ダイナミン１』の発見 Invited

山田浩司

"未来へのバイオ技術”勉強会（バイオインダストリー協会） 2021.4.23

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Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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Amphiphysin1はsynaptopodinと共同してアクチン制御に働く

山田浩司, The Mon La, 阿部匡史, 竹田哲也, 高島英造, 長岡ひかる, 淺沼克彦, 竹居孝二

第４回ポドサイト研究会 2020.3

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ダイナミンによる細胞骨格制御と創薬 Invited

薬学総合セミナー（新潟薬科大学） 2020.2.4

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Language：Japanese Presentation type：Oral presentation (invited, special)

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Dynamin1 / cortactin complex bundles actin filaments and supports growth cone formation.

Kohji Takei, Tadashi Abe, Hiroshi Yamada, Mari Sei

50th Annual meeting, American Society for Cell Biology 2010.12

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Novel mechanism of actin bundle formation by Dynamin1 / cortactin complex.

Hiroshi Yamada, Tadashi Abe, Yoshihiro Kawada, Kohji Takei

49th Annual meeting, American Society for Cell Biology 2009.12

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Microvesicle-mediated exocytosis of glutamate in pinealocytes. NIBB International meeting on dynamic aspects of lysosomal/vacuolar system.

Hiroshi Yamada, Akitsugu Yamamoto, Akihito Yamaguchi, Yoshinori Moriyama

NIBB International meeting on dynamic aspects of lysosomal/vacuolar system. 1997.12

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Microvesicle-mediated exocytosis of glutamate is a novel paracrine-like chemical transduction mechanism and inhibits melatonin secretion in pinealocytes.

Hiroshi Yamada, Akitsugu Yamamoto, Yoshinori Moriyama

Asiapacific pineal meeting. 1997.3

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Na+-dependent glutamate transporter in pinealocytes: identification and characterization as a reuptake system in the novel paracrine-like intercellular chemical transduction.

Hiroshi Yamada, Akitsugu Yamamoto, Yoshinori Moriyama

in Fifteen year's progress and future perspectives of vacuolar ATPases. 1996.11

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▼display all

Industrial property rights

アルツハイマー病治療薬

高橋智幸, Zacharie Taoufiq, 堀哲也, 竹居孝二, 山田浩司

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Application no：特願2022-145546 Date applied：2022.9.23

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抗がん剤

道上宏之, 松井秀樹, 林桂一郎, 竹居孝二, 山田浩司, 宮地弘幸, 浅井章良

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Applicant：国立大学法人岡山大学

Application no：特願2012-263317 Date applied：2012.11.30

Announcement no：特開2014-108930 Date announced：2014.6.12

Patent/Registration no：特許第5922563号 Date issued：2016.4.22

J-GLOBAL

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抗浸潤薬の新規スクリーニング法

山田浩司, 道上宏之, 竹居孝二, 松井秀樹, 浅井章良

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Applicant：国立大学法人岡山大学

Application no：特願2012-137489 Date applied：2012.6.19

Announcement no：特開2014-002043 Date announced：2014.1.9

Patent/Registration no：特許第5806168号 Date issued：2015.9.11

J-GLOBAL

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医薬組成物

竹居孝二, 李順愛, 山田浩司, 公文裕巳, 那須保友, 渡部昌実

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Applicant：国立大学法人岡山大学

Application no：特願2008-114729 Date applied：2008.4.25

Announcement no：特開2009-057364 Date announced：2009.3.19

Patent/Registration no：特許第5283962号 Date issued：2013.6.7

J-GLOBAL

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Awards

教育奨励賞

2011 岡山医学会

山田浩司

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脳神経研究奨励賞

2009 岡山医学会

山田浩司

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Research Projects

新規細胞分裂必須分子を標的とする革新的抗マラリア薬の開発

2025.04 - 2026.03

国立研究開発法人日本医療研究開発機構「橋渡し研究戦略的推進プログラム」シーズA

山田浩司、高島英造、内橋貴之、澤田隆介

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Authorship：Principal investigator

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熱帯熱マラリア原虫の細胞分裂を司る膜狭窄・分裂機構の解明と新規創薬ターゲットの創生

2025.01 - 2027.12

シオノギ感染症研究振興財団基礎基盤研究助成金

山田浩司、高島英造、内橋貴之、澤田隆介

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\30000000 （ Direct expense: \30000000 ）

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慢性腎臓病克服をめざす糸球体ポドサイトのマルチスケール解析

2024.06

岡山県電源所在県科学技術振興事業

山田浩司, 竹居孝二, 田邊克幸, 米澤朋子

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\7498150 （ Direct expense: \6816500 、 Indirect expense：\681650 ）

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腎臓糸球体ポドサイトの血液濾過機能におけるダイナミン関連タンパクによるアクチン制御の役割

2024

愛媛大学プロテオサイエンスセンター共同利用・共同研究拠点「プロテオインタラクトーム解析共同研究拠点(PRiME)」

山田浩司、高島英造

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\250000 （ Direct expense: \250000 ）

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ダイナミン超複合体による新規の細胞骨格リアレンジメント機構

Grant number：23H02477 2023.04 - 2026.03

日本学術振興会科学研究費助成事業基盤研究(B)

竹居孝二、山田浩司

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Authorship：Coinvestigator(s) Grant type：Competitive

Grant amount：\3000000 （ Direct expense: \3000000 ）

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マラリア原虫細胞分裂機構をターゲットとする新規創薬基盤の創生

2023

国立研究開発法人日本医療研究開発機構新興･再興感染症に対する革新的医薬品等開発推進研究事業

山田浩司、高島英造、内橋貴之、成田哲博、高野光則

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\39000000 （ Direct expense: \30000000 、 Indirect expense：\9000000 ）

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In vitro解析からひもとくダイナミンによる細胞骨格制御機構とその破綻による病態生理の解析

2023

愛媛大学プロテオインターラクトーム解析共同研究拠点共同研究

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\250000 （ Direct expense: \250000 ）

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The role of dynamin in actin dynamics in cancer cell migration and metastasis

Grant number：22K06580 2022.04 - 2025.03

日本学術振興会科学研究費助成事業基盤研究(C)

Tadashi Abe, Hiroshi Yamada

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Authorship：Coinvestigator(s) Grant type：Competitive

Grant amount：\800000 （ Direct expense: \800000 ）

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細胞骨格ダイナミクスに基づく分子輸送制御システムの解明と革新的癌創薬への新展開

Grant number：19H01064 2022.04 - 2024.03

日本学術振興会科学研究費助成事業基盤研究(A)

渡部昌実, 黄鵬, 那須保友, 定平卓也, 竹田哲也, 竹居孝二, 野口洋文, 山田浩司, 落合和彦

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Grant type：Competitive

Grant amount：\1400000 （ Direct expense: \1400000 ）

各種癌細胞を入手すると同時に、より普遍性の高い研究を遂行する為、独自のマウス間葉系幹細胞を樹立した。各種癌細胞において、細胞骨格因子が関わる細胞内分子輸送システムに重要と考えられるタンパク質群の発現を網羅的に解析した。特に、REIC/Dkk-3、SGTA、Tctex-1、Dyneinモーター、Dynaminおよびその他の細胞骨格（制御）因子に着目して、それら関連分子を含め発現を解析した。一部のタンパク質においてはその発現を認めず、免疫組織学的な解析を行うべく準備を進めた。これまでの男性ホルモンレセプターの核内移行に基づく実験系に加え、糖質コルチコイドレセプターの核内移行に基づく表現型解析系を立ち上げた。また癌創薬の観点から複数のDynamin阻害薬に関する検討を行い、in vivo投与での作用機序解明に係る動物実験での解析系の立ち上げを行った。

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細胞骨格及び接着安定化分子を標的とする糸球体ポドサイト保護剤の開発

2022.04 - 2024.03

国立研究開発法人日本医療研究開発機構「橋渡し研究戦略的推進プログラム」シーズA

山田浩司, 竹居孝二

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\5000000 （ Direct expense: \5000000 ）

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細胞骨格及び接着安定化分子を標的とする糸球体ポドサイト保護剤の開発

2022

国立研究開発法人日本医療研究開発機構「橋渡し研究戦略的推進プログラム」シーズA

山田浩司, 竹居孝二

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\2000000 （ Direct expense: \2000000 ）

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In vitro解析からひもとくダイナミンによる細胞骨格制御機構とその破綻による病態生理の解析

2022

愛媛大学プロテオインターラクトーム解析共同研究拠点共同研究

山田浩司、高島英造

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\250000 （ Direct expense: \250000 ）

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細胞膜とアクチン細胞骨格を繋ぐダイナミンの生理機能の解明

2021.04 - 2022.03

愛媛大学愛媛大学プロテオサイエンスセンター共同研究

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Authorship：Principal investigator Grant type：Competitive
ポドサイトが形成する血液濾過装置を支える新規アクチンリモデリング機構の解明

Grant number：20K08591 2020.04 - 2023.03

日本学術振興会科学研究費助成事業基盤研究(C)

山田浩司, 竹居孝二, 淺沼克彦

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Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

腎臓糸球体ポドサイトに発現しているダイナミン1及びダイナミン2の細胞内機能を調べることを目的とした。本年度は、ダイナミン２のアクチン制御機構について、in vitro解析を中心に行なった。ダイナミン２はCharcot-Marie-Tooth病(CMT)の原因遺伝子の一つであることが知られている。ヒトでは9つの変異が知られている。その一つであるK562Eが細胞内のストレスファイバーとアクチン線維束形成を異常にすることを見出している(Yamada et al., Neurosci. lett., 2016)。この現象を、in vitroで解析するために、ダイナミン２野生型とK562Eをコムギ無細胞タンパク合成系を用いて調製した。最初に、ダイナミン２K562Eの性状を調べた。変異体は、膜との結合と、膜結合に依存するGTPase活性が顕著に低下していた。低イオン強度緩衝液中での自己重合性のGTPase活性は、野生型のそれに比較して、30%低下していた。また、アクチン線維とダイナミン野生型またはK562Eを混合し、その形態を電子顕微鏡にて観察した。変異体は、野生型同様にアクチン線維を束化した。このアクチン線維を精査した。高速原子間力顕微鏡観察から、ダイナミンが螺旋状に重合しているリムの部分にアクチン線維が結合していることがわかった。ダイナミンにより形成されたアクチン線維束と細胞膜を模倣したリポソームとの結合を調べた。ダイナミン変異体により形成されたアクチン線維束はリポソームにほとんど結合しなかった。従って、変異体は、細胞内でアクチン線維を束化するものの細胞膜と結合できないために、ストレスファイバー形成不全がおこると考えられる。本研究は、Frontiers in Cell and Developmental Biologyに2022年5月10日に掲載された。

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ダイナミンをターゲットとする抗グリオーマ分子標的薬の開発

2020.04 - 2022.03

国立研究開発法人日本医療研究開発機構「橋渡し研究戦略的推進プログラム」シーズA

竹居孝二、山田浩司、道上宏之、佐藤あやの

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Authorship：Coinvestigator(s)

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ダイナミンによる細胞骨格制御機構とその破綻に関わる病態解明

2020.04 - 2021.03

愛媛大学愛媛大学プロテオサイエンスセンター共同研究

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Authorship：Principal investigator Grant type：Competitive
エンドサイトーシス関連分子のアクチン細胞骨格と膜制御の機能連関

2020.04 - 2021.03

神戸大学神戸大学バイオシグナル総合研究センター共同利用研究

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Authorship：Principal investigator Grant type：Competitive
エンドサイトーシス関連分子のアクチン細胞骨格と膜制御の機能連関

2019.07 - 2020.03

神戸大学神戸大学バイオシグナル総合研究センター共同利用研究

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Authorship：Principal investigator Grant type：Competitive
Elucidation of the function of novel actin-binding factors in neutrophil phagocytosis and extracellular trap formation

Grant number：19K07084 2019.04 - 2022.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

ABE TADASHI

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Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

Neutrophils play an essential role in the destruction of bacteria in innate immune system. In the process of killing pathogens, neutrophils drastically change their cell shape with accompanying the rearrangement of actin cytoskeleton. We found that expressing dynamin 2 K562E mutant, one of the pathogenic mutations in Charcot-Marie-Tooth disease in cells leads to aberrant actin clusters and stress fibers. The effect of this mutant on actin fibers was analyzed in vitro. Recombinant dynamin 2 K562E showed lower self-assembly ability and membrane binding ability than that of dynamin 2 wildtype. Although dynamin K562E directly bundled actin filaments, the formed bundles showed much less ability to bind to the lipid membranes as compared to dynamin 2 wildtype. In conclusion, dynamin 2-mediated interactions between actin and membranes are critical for actin bundle formation in neutrophil functions.

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Cooperative regulation of cytoskeleton and membrane dynamics by novel mechanism of dynamin

Grant number：19H03225 2019.04 - 2022.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

Takei Kohji

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Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

We found that Charcot-Marie-Tooth disease-associated mutations of dynamin 2 cause aberrant stress fibers, showing that dynamin is required for the formation and stabilization of stress fibers. And we reconstituted in vitro the actin bundle formation by dynamin. We also found that dynamin 1 bundles microtubules, and showed that this bundling is necessary for the primary processes formation and stabilization of cell morphology of renal glomerular podocytes. The microtubule-binding site of dynamin 1 was identified. Furthermore, regarding the regulation of membrane dynamics, we demonstrated that dynamin 2 and BIN1, a BAR protein, cooperatively function in T-tubule formation and stabilization of skeletal muscle cells.

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ダイナミンによる細胞骨格制御機構とその破綻に関わる病態解明

2019.04 - 2020.03

愛媛大学愛媛大学プロテオサイエンスセンター共同研究

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Authorship：Principal investigator Grant type：Competitive
Genome-wide expression of P. falciparum membrane proteins

Grant number：18K19455 2018.06 - 2020.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory) Grant-in-Aid for Challenging Research (Exploratory)

Takashima Eizo

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Grant amount：\6240000 （ Direct expense: \4800000 、 Indirect expense：\1440000 ）

Membrane associated plasmodial proteins are usually difficult to predict using the present algorithms, and are not well expressed by under conventional wheat germ cell-free protein expression system (WGCFS) conditions. In this study, we aimed at expressing P. falciparum genes using WGCFS-liposome method for membrane protein production. As a result, we succeeded to express P. falciparum proteins which are not well expressed by usual conditions of WGCFS. Moreover, liposome encapsulated WGCFS successfully expressed some membrane proteins and the recombinant proteins were localized to the liposomal membrane. Based on these results, we conclude that WGCFS-liposome is a useful tool for the expression of membrane proteins and will facilitate production and functional analyses of P. falciparum membrane proteins.

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ダイナミンによる細胞骨格制御機構とその破綻に関わる病態解明

2018.04 - 2019.03

愛媛大学愛媛大学プロテオサイエンスセンター共同研究

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Authorship：Principal investigator Grant type：Competitive
熱帯熱マラリア原虫ダイナミンホモログによる膜制御機構

2018.04 - 2019.03

神戸大学神戸大学バイオシグナル総合研究センター共同利用研究

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Authorship：Principal investigator Grant type：Competitive
Analysis of membrane deforming proteins in Malaria parasite

Grant number：17K08808 2017.04 - 2020.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

Yamada Hiroshi

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Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）

Malaria parasite live with erythrocyte and form parasitophorous vacuole (PV) around the parasite. And the parasite develops membrane trafficking system to get nutrients and transport many proteins. In this study, we tried to examine whether or not malarial proteins could participate in these membrane remodeling. We found that the candidate protein directly bound to liposomes and deformed them. The activity of membrane deformation by the protein was altered in the presence of GTP. We are now investigating the detailed mechanism of membrane deformation by the protein using electron microscopy and high speed atomic force microscopy.

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熱帯熱マラリア原虫ダイナミンホモログによる膜制御機構

2017.04 - 2018.03

神戸大学神戸大学バイオシグナル総合研究センター共同利用研究

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Authorship：Principal investigator Grant type：Competitive
熱帯熱マラリア原虫ダイナミンホモログによる膜制御機構

2016.07 - 2017.03

神戸大学神戸大学バイオシグナル総合研究センター共同利用研究

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Authorship：Principal investigator Grant type：Competitive
Development of the anti-invasive drug for treatment of malignant glioma by drug-repositioning of anti-depressant.

Grant number：16K10756 2016.04 - 2019.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

ABE TADASHI

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Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）

For treatment of malignant glioma, highly invasive glioma cells become obstacle to surgical removal of primary tumor. To suppress the high invasive activity of glioma cells, we identified the novel anti-invasive drug, a fluvoxamine, by drug repositioning of anti-depressant. Screening for more potent anti-invasive drugs using fluvoxamine as a lead compound are currently in progress. Furthermore, we found that actin-bundling by dynamin-cortactin complex is required for glioma cell invation. Cortactin is phosphorylated by cyclin dependent kinase 5 (CDK5), and its phosphorylation negatively regulates glioma cell invasion.

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Development of antimalarial drugs targeting the membrane trafficking

Grant number：26670201 2014.04 - 2017.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research Grant-in-Aid for Challenging Exploratory Research

YAMADA Hiroshi, TAKEI Kohji

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Grant amount：\3640000 （ Direct expense: \2800000 、 Indirect expense：\840000 ）

Malaria parasites live in erythrocyte by forming parasitophorous vacuole (PV) around the parasite, and by developing membrane trafficking system. In this study, we tried to examine whether or not malarial proteins could participate in these membrane remodeling. By microscopy, the candidate protein self-assembled under the low ionic strength conditions. Furthermore, the candidate protein deformed liposomes. The activity of membrane deformation by the protein was altered in the presence of GTP but not GTP gamma S, a non-hydrolyzable GTP analogue. We are now investigating the detail mechanism of membrane deformation by the protein using electron microscopy.

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The role of endocytosis in oateoarhthritis

Grant number：26670665 2014.04 - 2016.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research Grant-in-Aid for Challenging Exploratory Research

HIROHATA Satoshi, YAMADA Hiroshi, OHTSUKI Takashi

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Grant amount：\3640000 （ Direct expense: \2800000 、 Indirect expense：\840000 ）

The regulation of ADAMTS5 is crucial for osteoarthritis. However, little is known for its mechanism. We hypothesized that endocytosis may be involved in ADAMTS5 regulation. First, we found that endocytosis occurred in OUMS-27. We next examined endocytosis-related molecule, LRP-1 and RAP. The mRNA level of receptor-associated protein (RAP), antagonist of LRP-1, was not changed by IL-1 beta stimulation. It is interesting that the small-sized bands were found by Western blotting using anti-LRP-1 antibody after IL-1 beta stimulation. This is considered to be a short LRP-1 and the shedding of LRP-1 may be occurred by IL-1 beta stimulation.

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Multi-functionality of dynamin family and mechanism of integrated control

Grant number：23370089 2011.04 - 2014.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

TAKEI Kohji, YAMADA Hiroshi, TANEBE Kenji

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Grant amount：\18980000 （ Direct expense: \14600000 、 Indirect expense：\4380000 ）

We have clarified a novel regulatory mechanism of actin dynamics by dynamin/cortactin complex. The complex is ring-shaped and it changed the conformation upon dynamin GTP hydrolysis, from open ring to close ring. By the open-cloze motion, the complex bundled F-actins and stabilized the actin bundles. Furthermore, we identified N'-[4-(dipropylamino)benzylidene]-2-hydroxybenzohydrazide(DBHA)as a dynamin inhibitor that suppress dynamin-dependent actin regulation. DBHA inhibited recruitment of dynamin to the leading edge of migrating cancer cell line and ruffle formation. It also showed inhibitory effect in cell migration, invasion, and proliferation.

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Development of antimalarial drug targeting plasmodium falciparum dynamin

Grant number：23659213 2011 - 2013

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research Grant-in-Aid for Challenging Exploratory Research

TAKEI Kohji, YAMADA Hiroshi

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Grant amount：\3770000 （ Direct expense: \2900000 、 Indirect expense：\870000 ）

By in vitro actin polymerization assay, we identified N'-(4-(diethylamino) benzylidene)-4-methoxybenzohydrazide (DBHA) as a dynamin inhibitor. Effects of DBHA on ruffle formation and cell migration were also examined, and the results on DBHA were published in a journal article.
Dynamin isoforms present in plasmodium falciparum, pfDyn1 and pfDyn2, were expressed in insect cells, and they were purified. Using these recombinant proteins, we demonstrated that both pfDyn1 and pfDyn2 have GTPase activity. It was also shown that pfDyn1 and pfDyn2 have an ability to deform lipid membranes. Furthermore, pfDyn2 inhibitor candidate molecules were determined by GTPase activity assay-based drug screening.

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Elucidation of Biological Adaptation and Remodeling Mechanisms to Mechanical Stress Based on the Development of Novel Micro-Electro-Mechanical Systems (MEMS) technology

Grant number：22240056 2010 - 2012

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A) Grant-in-Aid for Scientific Research (A)

NARUSE Keiji, MOHRI Satoshi, NAKAMURA Kazufumi, TAKEI Kohji, YAMADA Hiroshi, IRIBE Gentaro, KATANOSAKA Yuki

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Grant amount：\50570000 （ Direct expense: \38900000 、 Indirect expense：\11670000 ）

Physical and mechanical stimuli such as gravity, extension, and shearing stress are generated throughout a living body. It has been gradually revealed that these stimuli, which are transmitted via the mechanotransduction mechanisms of cells, are not simply detrimental stresses for living organisms, but rather are biological information essential to developmental processes and functional adaptation of organs. In this project, on the basis of the development of original loading systems for mechanical stress to cells and tissues, the cellular adaptive responses to the mechanical stimuli and their transduction mechanisms in a variety of mechanosensitive tissues are to be examined. Thus, the project aims toelucidate the molecular mechanisms and roles of mechanotransduction system, which is utilized as a basis of many physiological events. It can also contribute to develop therapeutic approach to cancer invasion, cardiac hypertrophy, and regeneration of neuronal circuit.

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The role of dynamin in actin dynamics: Development of anticancer drugs inhibiting dynamin function

Grant number：22501013 2010 - 2012

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

ABE Tadashi, YAMADA Hiroshi, WATANABE Masami, ASAI Akira

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Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）

In search of more effective inhibitor for dynamin than dynasore, one hundred eighty one dynasore analogues were screened by in vitro actin polymerization assay. N'-(4-(diethylamino)benzylidene)-4-methoxybenzohydrazide (DBHA) was identified as a potent dynamin inhibitor. DBHA strongly inhibited the serum-stimulated ruffle formation, cell migration and invasion. Under these conditions, DBHA showed little toxicity for cultured cancer cell line. Furthermore, we clarified the function of dynamin/cortactin complex in the regulation of actin dynamics. The dynamin/cortactin complexes bundled several actin filaments and the bundling stabilized actin filaments. The actin bundling by dynamin/cortactin complex was necessary for formation of growth cone filopodia and cell migration. These findings might be crucial for developping anti cancer drugs.

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The relationship between actin bundling by dynamin/cortactin complex and stretch-activated cation channel on actin cytoskeleton

Grant number：22616004 2010 - 2012

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

YAMADA Hiroshi

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Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

The function of stretch-activated cation channel on actin dynamics was investigated. Calcium ion concentration in the ruffle membrane was increased during cell migration. Knockdown of the channel in cancer cell line resulted in marked reduction ofboth calcium influx via the channel and serum-stimulated ruffle formation, which were required for cell migration. These results suggest that stretch-activated cation channel in cancer cell is involved in cell migration possibly by regulating actin dynamics. Furthermore, we identified the function of dynamin/cortactin complex in the regulation of actin dynamics. The dynamin/cortactin complexes bundled several actin filaments and the bundling stabilized actin filaments. The actin bundling by dynamin/cortactin complex was necessary for formation of growth cone filopodia and cell migration. These findings might be crucial for the study for neuronal regeneration and cancer cell migration.

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Molecular mechanism of endocytosis that regulates membrane dynamics

Grant number：17370071 2005 - 2007

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

TAKEI Kohji, YAMADA Hiroshi, LI Shun-ai, TANAE Kenji

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Grant amount：\15280000 （ Direct expense: \14800000 、 Indirect expense：\480000 ）

In order to elucidate the role of endocytic proteins in membrane dynamics, localization and intracellular dynamics of dynamin and amphiphysin during phagocytosis were examined. For this purpose, testicular Sertoli cell phagocytosis was stimulated by phosphatidylserine containing liposomes, and intracellular localization and dynamics of the endocytic proteins were examined. By immunofluorescence and by live cell imaging, both dynamin2 and amphiphysin were concentrated at the leading edge of lamellipodia and ruffles. Ruffle formation, actin formation, and phagocytosis were markedly inhibited in Amphiphysin siRNA treated cells indicating that amphiphysin is essential for these processes. Furthermore, these effects in the amphiphysin 1-knocked down cells were rescued by co-overexpression of constitutive active Rac 1, suggesting that Rac 1 is involved in the process at the downstream of amphiphysin.
Next, effect of amphiphysin 1 on actin polymerization activity was examined in vitro. For this purpose, mouse testis cytosol supplemented with pyrene-conjugated actin, were subjected to quantitative actin in vitro polymerization assay. Actin polymerization was also observed under fluorescent microscopy using cytosol supplemented with rhodamine-conjugated actin. Actin assembly activity was considerably reduced in cytosol from amphiphysin 1 knock out mice, which can be recovered by adding back recombinant proteins.
Thus, amphiphysin 1, an endocytic protein, play a role in the regulation of actin dynamics, by which it stimulate in membrane dynamics and phagocytosis.

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エンドサイトーシスの分子機構:分子構造から細胞機能まで

Grant number：15079206 2003 - 2007

日本学術振興会科学研究費助成事業特定領域研究

竹居孝二, 山田浩司, 李順愛, 田邊賢司, 絹田正裕

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Grant amount：\106400000 （ Direct expense: \106400000 ）

1)ダイナミンによる微小管動態制御
エンドサイトーシスの機能タンパクであるダイナミンは、細胞骨格の一つである微小管に結合する遺伝子として同定されたが、その生理的意義は不明であった。そこで我々はRNAiによりダイナミン2の発現を抑制した細胞の微小管を形態的に観察した。その結果、ダイナミン2のノックダウンにより動的な微小管が減少し、微小管に沿った膜輸送の障害によりゴルジ体の分散が起こる事を見いだした。さらに、変性性末梢神経障害であるシャルコー・マリー・トゥース病の原因遺伝子として報告されたダイナミン変異体を発現させた細胞でも、同様の結果が観察され微小管の異常な蓄積が観察された。以上の結果からダイナミンが微小管のダイナミクスを制御している可能性が示唆された。
2)チューブ状エンドソームの切断と成熟機構
分子選別に機能する初期エンドソームでは、初期エンドソームからリサイクルに向かうチューブ状エンドうソームが形成切断され、リサイクルエンドソームに向かう。一方、残存したエンドソームは、分解に向かう物質を含んだ後期エンドソームへの成熟が行われる。この二つのプロセスがどのように連携しているのかは判っていなかった。我々は、阻害剤を用いてチュニブ状エンドソームの切断、後期エンドソームへの成熟を阻害し、これらのプロセスについて解析した。その結果、チュブ状エンドソームの切断にダイナミンが関与しており、チューブ切断がエンドソームの酸性化と移動などの成熟過程に必要である事を見出した。

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エンドサイト-シス小胞形成のリアルタイム観察

Grant number：15657028 2003 - 2005

日本学術振興会科学研究費助成事業萌芽研究

竹居孝二, 山田浩司, 李順愛, 絹田正裕

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Grant amount：\2900000 （ Direct expense: \2900000 ）

ファゴサイトーシスの分子機構解明のために、セルトリ細胞を用いてファゴサイトーシスのリアルタイム観察を行った。まず、セルトリ細胞株SerW3にフォスファチジルセリン(PS)を認識する受容体(SR-B1)が発現していることをウエスタンブロットにより確認し、細胞膜における局在のパターンを蛍光免疫染色により明らかにした。次に、SerW3細胞によるPS含有大型リポゾーム取込みを経時的、定量的に調べることにより、セルトリ細胞のファゴサイトーシスが、SR-B1を介してPS依存性に起こることを明らかにした。SerW3細胞に、PS含有リポゾームあるいはPSで被覆したスチレンビーズを貪食させ、その様子を超高速共焦点レーザー顕微鏡下でリアルタイム観察したところ、ファゴサイトーシスにおけるpseudo pod形成に先立って糸状突起やラッフルの形成など、F-アクチンの再編成による細胞膜の形態変化が著明に認められた。PS刺激からアクチン重合、ラッフル形成にいたる経路には、フォスファチジルイノシトール3リン酸キナーゼ、Rac、cdc42が関与することが阻害剤を用いた実験により示された。GFP-アンフィファイジン1を発現させたSerW3細胞をライブイメージングにより観察すると、ラッフル形成に伴ってアンフィファイジン1がラッフルに集積する様子が認められた。SerW3細胞のアンフィファイジン1をRNAiによりノックダウンするとラッフル形成が抑制されたことから、アンフィファイジン1がファゴサイトーシス初期におけるアクチンフィラメントの再編成に関与することが示唆された。

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Molecular mechanism of L-glutamate and GABA-mediated regulation of secretion of glucagon and insulin in islets of Langerhans

Grant number：15390026 2003 - 2005

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

MORIYAMA Yoshinori, OTUSKA Masato, YAMADA Hiroshi

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Grant amount：\13500000 （ Direct expense: \13500000 ）

Vesicular glutamate transporter (VGLUT) and vesicular GABA transporter (VGAT) play essential roles in the glutamatergic and GABAergic chemical transduction, respectively. VGLUT and VGAT are present not only in the neuronal synaptic vesicles but also in the glucagon-containing secretory granules of A cells of islets of Langerhans, and are responsible for vesicular storage of glutamate and GABA, respectively. This research aimed to reveal the molecular mechanism of the glutamate- and GABA-mediated regulation of secretion of glucagon and insulin in the islets. During three years, we have obtained the following important results.
(1)We have revealed topology of VGLUT2.
(2)We have established an in vitro assay system for VGLUT, and identified some essential amino acid residues for transport of glutamate as well as targeting.
(3)We have established an in vitro assay system for VGAT.
(4)We have observed decreased glutamatergic signaling in VGLUT1 KO mice.
(5)We have identified and characterized glutamate signaling pathway in intestinal L cells.
(6)We have identified and characterized a series of receptors for regulation of secretion of insulin and glucagon.

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A Study on Regulatory Mechanisms of Endocytosis

Grant number：14380336 2002 - 2004

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

TAKEI Kohji, YAMADA Hiroshi

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Grant amount：\11100000 （ Direct expense: \11100000 ）

Regarding to regulatory mechanisms of endocytosis, the followings were revealed.
1.Regulation of endocytosis by Amphiphysin 1
Dynamin-dependent endocytosis can be reconstituted in vitro by incubating large unilammelar liposomes with brain cytosol or dynamin in presence of GTP. Using amphipshyin knockout brain cytosol in this experimental system, it was clarified that amphiphysin 1 stimulates dynamin GTPase activity and thereby enhances dynamin-dependent vesicle formation. This effect required both BAR domain and SH3 domain of amphiphysin 1. Low membrane curvature of large liposomes was also requisite for the stimulatory effect of amphiphysin 1.
2.Regulation of endocytosis cdk5-dependent phosphorylation
Both dynamin 1 and amphiphysin 1 are phosphorylated by cyclin dependent kinase 5 (cdk5). Incubation of liposomes with phosphorylated dynain and phosphorylated amphiphysin 1 in presence of GTP resulted in few vesicle formation, whereas dephophorylated proteins massively generated vesicles. Thus, endocytosis is likely to be regulated by cdk5-dependent phosphorylation.
3.Localization of dynamin 2 and dynamin3
Distinct localization of dynamin 2 and dynamin3 in Sertoli cells was revealed suggesting different functions of these isoforms.

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シナプスにおけるエンドサイトーシス機能タンパク-膜リン脂質相互作用の解析

Grant number：14380306 2002 - 2004

日本学術振興会科学研究費助成事業基盤研究(B)

絹田正裕, 竹居孝二, 山田浩司

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Grant amount：\10700000 （ Direct expense: \10700000 ）

Amphiphysin 1によるDynamin GTPase活性の上昇
Dynamin 1GTPアーゼ活性に対するAmphiphysin 1と膜脂質の影響を調べ、Amphiphysin 1が、大型リポゾームの存在下にDynamin 1のGTPアーゼ活性を上昇することを明らかにした。リポゾームにフォスファチジルセリン、フォスファチジルイノシトール2リン酸などの酸性リン脂質を含まれる場合、特にGTPアーゼ活性の増強効果が高かった。ミュータントAmphiphysin 1を用いた解析により、Dynamin 1GTPアーゼ活性の増強には膜脂質への結合を担うBARドメインと、dynaminとの結合ドメインであるSH3ドメインが必要でであることを明らかにした。また、クラスリン結合部位、AP2結合部位を含む中間部は、Dynamin 1GTPアーゼ活性に対して抑制的に働くことが示唆された。
Amphiphysin 1によるDynamin 1と膜脂質の結合の増加
リポゾームを用いた結合実験により、Amphiphysin 1がDynamin 1と膜脂質の結合を増加させることを明らかにした。この結合増加の一因は、Amphiphysin 1のBARドメインとSH3ドメインを介した間接的結合によるものであることを、ミュータントAmphiphysin 1を用いた解析により明らかにした。
Amphiphysin 1とDynamin 1と脂質膜の結合増加
ミュータントAmphiphysin 1を用いた解析により,Amphiphysin 1とDynamin 1のリング形成にはBARドメインとSH3ドメインが必要であることを明らかにした。また、リング形成とDynamin 1 GTPアーゼ活性の上昇が、Dynamin 1と膜結合性にある程度相関することを示唆した。
以上の成果を論文にまとめ発表した。

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膵島におけるグルタミン酸によるインスリン分泌制御機構の解析

Grant number：14770600 2002 - 2004

日本学術振興会科学研究費助成事業若手研究(B)

山田浩司

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Grant amount：\3800000 （ Direct expense: \3800000 ）

株化細胞であるMIN6細胞に、AMPA型グルタミン酸受容体のイソフォームであるGluR2が発現していることを免疫化学的手法を用いて見いだした。グルタミン酸受容体のエンドサイトーシスを生化学的に検出する手法を構築し、GluR2が、AMPA刺激により細胞内にエンドサイトーシスされることを見いだした。このエンドサイトーシスは、細胞外グルコースが低濃度の場合に顕著に起こり、AMPA型グルタミン酸受容体のアンタゴニストであるGYKI52466により阻害されることが判明した。さらに、このリガンド依存性エンドサイトーシスは、細胞外のNaイオン、Caイオンを除くことにより阻害された。さらに、人工的に細胞膜の脱分極を高濃度のKClを加えることにより、誘導するとGluR2のエンドサイトーシスが起こった。従って、GluR2のエンドサイトーシスは、グルタミン酸の受容体への結合と細胞膜の脱分極が必要であることが示唆された。さらに、蛍光ラベルしたGluR2(GFP-GluR2)をMIN6細胞に強制発現させ、AMPA刺激で、GluR2のエンドサイトーシスを反映していると考えられる蛍光スポットの移動が観察できた。

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神経シナプスにおけるエンドサイトーシスの変化を指標とした神経変性疾患の解析

Grant number：13035032 2001 - 2002

日本学術振興会科学研究費助成事業特定領域研究

竹居孝二, 山田浩司, 絹田正裕

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Grant amount：\4600000 （ Direct expense: \4600000 ）

1.老化モデルマウスを用いた解析
老化脳における神経シナプスの変化を調べるため、老化モデルマウスであるKlotho変異マウス海馬シナプスにおけるタンパク発現を精査した。蛍光免疫染色法および蛍光強度の定量的測定では、シナプス小胞のマーカーであるSynaptophysinの発現が海馬CA3領域において軽度減少していた。次にシナプスエンドサイトーシス機能蛋白(Clathrin、AP2、Dynamin1、Amphiphysin1など)の発現と局在をそれぞれウエスタンブロッティング、蛍光免疫染色法により調べた。Klotho変異マウス海馬ではDynamin1の発現がわずかにの減少していたが、他のタンパクについては変化はみられなかった。
2.Klothoタンパクの局在
Klotho変異マウス脳における上記の変化とKlothoタンパクの関連を明らかにする目的で、Klothoタンパクの局在を蛍光免疫染色法により調べた。既にKlotho mRNAは脈絡叢上皮に局在することが報告されているが、Klothoタンパクも脈絡叢に特異的に発現していた。さらに共焦点顕微鏡観察、金コロイドを用いた凍結免疫電顕法により、Klothoタンパクが脈絡叢上衣細胞のapical側(脳室側)に選択的に局在することが明らかになった。脈絡叢は脳脊髄液の産生、調節に機能することから、Klotho変異マウス脳にみられた上記の比較的軽度な変化は、脳脊髄液産生、調節の変化を介した二次的な変化である可能性が示唆された。

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AMPA型グルタミン酸受容体のエンドサイトーシスの分子機構

Grant number：13041045 2001

日本学術振興会科学研究費助成事業特定領域研究(A)

竹居孝二, 山田浩司, 絹田正裕

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Grant amount：\4000000 （ Direct expense: \4000000 ）

1.膵島β細胞株MIN6のキャラクタライゼイション
膵島β細胞にはAMPA受容体が発現していることが見い出されており、本研究ではAMPA受容体のエンドサイトーシスの機構を解明するためにβ細胞を用いることを提案した。
まず特異的抗体を用いたWestern blot法により、MIN6におけるAMPA受容体の発現を明らかにした。次にMIN6におけるエンドサイトーシス機能タンパクの発現を調べた。その結果、クラスリン依存性エンドサイトーシスの機能タンパクであるアンフィファイジン、さらにアンフィファイジンのリン酸化酵素CDK5が高発現することがWestern blot法により見い出された。
2.エンドサイトーシスのin vitro再構成系の確立
AMPA受容体エンドサイトーシスの分子メカニズム解明のためには、エンドサイトーシスによる取り込み小胞の形成をin vitroで再現する実験系の確立が必要であると考えた。In vitroで人工脂質膜(リポソーム)を膜成分として、ATPおよびGTP存在下に細胞質と反応させることにより、大型(直径>1μm)のリポソームから直径100nm以下の小胞が多数形成される実験系を確立した。さらに、形成された小胞の大きさ、数、相対的質量を動的光拡散測定装置を用いて測定することにより、小胞形成の定量化を可能とした。この実験系はAMPA受容体の選択的取り込みを解析するための実験系として応用されうる。

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A Study on Molecular Mechanisms of Endocytosis

Grant number：12480217 2000 - 2001

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

TAKEI Kohji, YAMADA Hiroshi, KINUTA Masahiro

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Grant amount：\14100000 （ Direct expense: \14100000 ）

Establishment of in vitro cell-free system: In order to elucidate molecular mechanisms involved in endocytosis, vesicle formation in endocytosis was reconstituted in vitro. Incubation of large liposomes, larger than 1 μm in diameter, with brain cytosol resulted in massive formation of small vesicles, smaller than 100 nm in diameter. The vesicle formation required both ATP and GTP. Vesicle formation was drastically reduced when liposomes were incubated with dynamin 1 -depleted cytosol, indicating that vesicle formation in this experimental system represents endocytic vesicle formation. The vesicle formed during the incubation can be analyzed quantitatively and qualitatively by dynamic light scattering.
Functions and Kinetics of membrane lipids: Functions of membrane lipids were studied by analyzing vesicle formation from liposomes of with various compositions. Vesicle formation increased as phosphatidylinositol-4.5-bisphosphate (PIP_2) concentration in liposomes was increased. Furthermore, PIP_2 was degraded to phosphatidylinositol-4-bisphosphate, then to phosphatidylinositol. Next, PIP_2 synthesis during the reaction was analyzed by addition of neomycin, inhibitor for PIP_2 degradation, in the reaction mixture. PIP_2 synthesis was increased by active form of ADP-ribosylation factor 6 (Arf6). It was suggested that increase of membrane recruitment of AP2, clathrin adaptor protein, by Arf6 might attribute to the increase of PIP_2 synthesis.
Kinetics of membrane lipids in culture cells: Degradation of PTP_2 synthesis upon endocytosis was examined in culture cells. Metabolically labeled HeLa cells were stimulated for endocytosis and the amount of PIP_2 was analyzed. Similar PIP_2 degradation as that observed in the cell-free system was observed.

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遺伝子、蛋白の機能、形態解析のための細胞内超微構造のデータベースの構築

Grant number：12208033 2000

日本学術振興会科学研究費助成事業特定領域研究(C)

竹居孝二, 横田一正, 山田浩司, 絹田正裕, 劉渤江, 國島丈夫

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本研究の目的は既報論文の電子顕微鏡像を形態的特徴によって分類し、そのデータベースを構することである。本年度の研究実績は以下の通り。
1 論文検索および電子顕微鏡像の解析
今年度は対象論文を神経細胞に限定し、まずPubMedで『neuron(神経)』および『ultrastructure(微細構造)』のキーワードにより検索される約3万7千余の論文の一部について、本データベースの対象論文としての妥当性を分析した。その結果、対象となる電子顕微鏡像は一部の学術雑誌に集中して掲載されていることが判明したので、効率的なデータベース構築をするため、学術雑誌ごとに論文の分析、入力作業を行うこととした。現在、Cell Tissue Researchに掲載された1197件の論文について、電顕像をその特徴により分類し、画像入力およびPubMedからの書誌情報の取り込みを行っている。
2 データベース構築
今年度はデータベース構築のためのプロトタイプシステムの実装を行った。このシステムでは、PubMedから得られる論文の書誌情報、論文からスキャナで取り込んだ画像データ、および画像データの特徴をあらわすキーワードなどをデータベースに格納し、相互の関連付けを半自動で行っている。データベースに対する検索は、要求が最も高いと思われるキーワードからの検索を中心に実装した。PubMedの書誌情報がASN.1形式で構造化されているため、テキスト情報は構造化文書の標準規格であるXML形式で構造化した上でデータベースに格納するという構成を採用した。その結果として、データベースとしてはXML文書処理機能の豊富なOracle8iを、また検索システムはJava言語のServlet機能を用いたWWWサーバ・クライアント方式をそれぞれ採用した。この知見を元に、来年度はより使いやすいデータ入力方式や検索方法、PubMedの更新への自動追随などを検討していきたい。

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松果体細胞がグルタミン酸作動性のパラニューロンであることの発見とその性格づけ

Grant number：97J03629 1998

日本学術振興会科学研究費助成事業特別研究員奨励費

山田浩司

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Grant amount：\1300000 （ Direct expense: \1300000 ）

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