Faculty Profiles - KIMURA Koji

MISC - KIMURA Koji

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ウシの発情周期中における卵管自発収縮の制御機構

山本ゆき, 山本ゆき, 小川泰司, 黒川真帆, 窪田早耶香, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020

暑熱ストレスは小胞体ストレスを介して子宮内膜間質細胞のIL6発現を増加させる

酒井駿介, 山本ゆき, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020

Pregnancy-specific expression of IFIT1 in the bovine external uterine mucosa

ASAOKA Natsuki, KIMURA Koji, TAKAHASHI Masashi, KUNII Hiroki, KOYAMA Keisuke, KUBO Tomoaki, HAMAGUCHI Yu, OGAWA Hidehiko, KOBAYASHI Hisato, BAI Hanako, KAWAHARA Manabu

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j129 - j129 2019.9

【Female genital tractの収縮を再考する】卵管の平滑筋収縮

木村康二

HORMONE FRONTIER IN GYNECOLOGY 26 ( 3 ) 185 - 189 2019.9

Language：Japanese Publisher：(株)メディカルレビュー社

卵管の平滑筋の運動は配偶子や胚の輸送を担う重要な機能の1つである。卵管の平滑筋は自律的な収縮弛緩による蠕動運動を行うことで輸送機能は果たしている。消化管と同じくある種のペースメーカー細胞の存在が示唆されているが、その詳細はいまだ解明されていない。また、卵管の平滑筋による輸送機能は性周期に依存して変化しており、これは卵巣由来の性ステロイドホルモンの直接または間接的な影響によるものと考えられている。(著者抄録)

DOI： 10.34449/j0015.26.03_0029-0033

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Progress of ciliogenesis in bovine oviductal epithelium preovulatory phase of the estrous cycle

ITO Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j83 - j83 2019.9

The effect of heat stress on macrophage chemotaxis in bovine endometrial cells

SAKAI Shunsuke, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j128 - j128 2019.9

Expression analysis of stem cell markers in bovine endometrial side population cells

TATEBAYASHI Ryoki, NAKAMURA Sho, MINABE Shiori, FURUSAWA Tadashi, ABE Ryoya, MORITA Yasuhiro, OHKURA Satoshi, KIMURA Koji, MATSUYAMA Shuichi

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j133 - j133 2019.9

Mechanism for regulation of calcium oscillation in cultured bovine oviductal smooth muscle cells

YAMAMOTO Yuki, KUROKAWA Maho, KIMURA Koji

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j134 - j134 2019.9

ウシ主席卵胞および次席卵胞の顆粒層細胞において発現する遺伝子の網羅的解析

大下雪奈, 渡辺雄貴, 春日崇, 中平陽子, 棟朝亜理紗, 木村康二, 松山秀一, 大蔵聡, 真方文絵, 松田二子

日本獣医学会学術集会講演要旨集 162回 439 - 439 2019.8

Survey of active site of interferon-tau by treating chemically synthetic peptides in endometrium cells

SUZUKI Toshiyuki, KIMURA Koji, BAI Hanako, KAWAHARA Manabu, TAKAHASHI Masashi

Hokkaido Journal of Livestock and Grassland Science 7 ( 1 ) 7 - 15 2019

Language：English Publisher：Hokkaido Society of Livestock and Grassland Science

<p>Interferon-tau (IFN-τ:195 aa) is a unique protein secreted only from ruminant and plays a very important role of pregnancy recognition. However, the source of IFN-τ is recombinant IFN-τ using E.coli expression system and it is very difficult to obtain. Therefore basic research and application research for improving pregnancy is highly limited. Besides, use of recombinant IFN-τ for field application is strictly regulated by biosafety and public consensus. Therefore, the purpose of this research is to discover the active site of IFN-τ by using of synthetic peptides as a substitute for recombinant IFN-τ. Eleven peptides (long chain: 27-28 aa, short chain: 7-17 aa) were chemically synthesized from amino acid sequence of IFN-τ. To confirm the agonistic activity of the synthetic peptides, single or mixed peptides were added to cultured bovine endometrium stromal cells to detect gene expression of interferonstimulated genes (ISGs). Recombinant IFN-τ was also added as a positive control. Next experiment was performed to confirm the antagonistic activity of the synthetic peptides. Single or mixed peptides was added to stromal cells with recombinant IFN-τ followed by detecting of ISGs expression. IFN-τ significantly stimulated ISGs expression, whereas all peptides did not stimulate. Besides, addition of single or mixed peptides with IFN-τ showed no inhibitory effect. Overall results suggest that synthetic peptides would not have sufficient agonistic and antagonistic ability or lost the binding ability to IFN receptor by the possible structural change or no activity site having.</p>

DOI： 10.32253/hlgs.7.1_7

ウマ子宮内膜におけるPGF2α自己増幅機構

香西圭輔, 徳山翔太, Szostek Anna Z, 登石裕子, 角田修男, 田谷一善, 阪谷美樹, 高橋昌志, 南保泰雄, Skarzynski Dariusz J, 山本ゆき, 木村康二, 奥田潔

馬の科学 = Equine science 56 ( 4 ) 282 - 293 2019

暑熱環境下におけるウシ子宮内膜細胞のマクロファージ走化性物質産生の変化

酒井駿介, 山本ゆき, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

卵管自発収縮に関わるイオンチャネルと培養平滑筋細胞内カルシウム変動

山本ゆき, 山本ゆき, 黒川真帆, 小川泰司, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

着床前ウシ頸管および外子宮口周辺粘膜におけるインターフェロン誘導遺伝子の発現

国井宏樹, 伊藤月乃, 小木曽貴季, 鈴木惇文, 古山敬祐, Md. Abdus, Shabur Talkder, Balboula Ahmed Zaky, 唄花子, 川原学, 永野昌志, 木村康二, 高橋昌志

北海道牛受精卵移植研究会会報 37 17 - 17 2018.8

ウシ黄体におけるbetaglycanの役割とその発現調節

長谷川啓喜, 山下真路, 伊藤典彦, 岡本芳晴, 窪友瑛, 伊賀浩輔, 木村康二, 菱沼貢, 奥田潔, 西村亮, 西村亮

日本畜産学会大会講演要旨 124th 205 - 205 2018.3

ウシ伸長期胚の非外科的移植技術—Non-surgical Transfer Method of Elongating Bovine Conceptus

木村, 康二

岡山大学農学部学術報告 107 19 - 24 2018.2

Language：Japanese Publisher：岡山大学農学部

The objectives of the present study were to establish a non-surgical transfer method for elongating bovine conceptuses and to combine this method with biopsy and sexing. Bovine conceptuses were recovered from donor cows on day 13-14 of the estrus cycle. In experiment 1, day 13 conceptuses were transferred to recipient cows using a standard day 7 embryo transfer (ET) method. The pregnancy rate of day 13 conceptus transfer (CT) is comparable to that of day 7 ET. In experiment 2, day 14 conceptuses were transferred using modified methods (balloon catheters or ET guns with modified sheaths). Using the standard ET method, no pregnancies were obtained;however, when balloon catheters or ET guns with modified sheaths were used, the pregnancy rates after CT were 48.0％ and 44.8％, respectively. In experiment 3, day 14 conceptuses were biopsied without a micromanipulator, sexed using the loop-mediated isothermal amplification (LAMP) method, and transferred to recipient cows. The pregnancy rate of biopsied conceptuses was 46.2％ and did not differ significantly from that of unbiopsied with conceptuses. Moreover, all pregnant cows transferred conceptuses following biopsy and sexing delivered calves with the expected sexes. These results suggested that the non-surgical bovine conceptus transfer (CT) method was comparable to day 7 ET, and that this technique enables biopsy and sexing without expensive equipment such as a micromanipulator or specialized skills.

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Effect of heat stress on IFNT responsiveness in bovine uterine epithelial cells

KOMATSU Seimei, SUZUKI Toshiyuki, KUNII Hiroki, KAWAHARA Manabu, KIMURA Koji, TAKAHASHI Masashi, BAI Hanako

The Journal of Reproduction and Development Supplement 111 ( 0 ) P - 38-P-38 2018

TRP channels are localized on bovine endometrium and involved in increase of prostaglandin production of bovine endometrial stromal cells under heat stress

YAMADA Ai, TAKAMI Keito, SAKAI Shunsuke, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 64 ( Suppl Japanese Issue ) P-39 - P-39 2018

Evaluation of the ion channels involved in phasic spontaneous contraction and intracellular calcium signaling in cultured smooth muscle cells of bovine oviducts

YAMAMOTO Yuki, KUROKAWA Maho, OGAWA Taiji, KIMURA Koji

The Journal of Reproduction and Development Supplement 64 ( Suppl Japanese Issue ) OR2-14 - OR2-14 2018

Estimation of differentiation pathway by charactarization of bovine oviductal epithelial cells

ITO Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 64 ( Suppl Japanese Issue ) P-40 - P-40 2018

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