Faculty Profiles - KIMURA Koji

MISC - KIMURA Koji

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ウシの子宮内膜老化細胞に対するBPTESの効果と作用機序の解明

千田鈴乃, 市川怜, 山田直季, 中村翔, 大蔵聡, 伊東楓, 杉野耀亮, 木村康二, 木村康二, 松山秀一

日本分子生物学会年会プログラム・要旨集(Web) 47th 2024

Tubule structure formation of bovine uterine glands in a 3D culture system

杉野耀亮, 藤原希, 山本ゆき, 木村康二

組織培養研究(Web) 42 ( 2 ) 2024

Directly regulation of oviductal smooth muscle contractility by estradiol-17β

窪田早耶香, 大河原里紗, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 46th 2023

Effects of the gel composition and additives on morphology of bovine uterine glands in 3D-culture

杉野耀亮, 佐藤太紀, 藤原希, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 46th 2023

ウシ未成熟精巣の器官培養における精原細胞の増殖とセルトリ細胞機能の評価

清水亮佑, 山本ゆき, 木村康二, 永岡謙太郎

日本畜産学会大会講演要旨 131st 2023

牛過剰排卵処置の簡易化

木村康二

臨床獣医 41 ( 7 ) 2023

The morphological change of bovine uterine gland induced by epidermal growth factor in 3D culture system

杉野耀亮, 佐藤太紀, 藤原希, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 45th 2022

Development of culture method for oviductal smooth muscle cells that maintain contractility

窪田早耶香, 山本ゆき, 山本ゆき, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 45th 2022

ウシ子宮腺による精子誘引

渡邊りお, 渡邊りお, 宗友真帆, 杉野耀亮, 木村康二

日本畜産学会大会講演要旨 130th 2022

Changes in proportion of side population cells and gene expression in bovine endometrium after intrauterine administration of povidone-iodine

TATEBAYASHI Ryoki, NAKAMURA Sho, KITAGAWA Yuri, KAJISA Miki, ICHIKAWA Rei, MORITA Yasuhiro, OHKURA Satoshi, KIMURA Koji, MATSUYAMA Shuichi

The Journal of Reproduction and Development Supplement 114th P-59 - P-59 2021

The formation of bovine uterine gland-like structure in 3D-culture and its related factor.

杉野耀亮, 佐藤太紀, 藤原希, 山本ゆき, 山本ゆき, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 44th 2021

The character of three-dimensional cultured bovine epithelial cells differs from in vivo tissues

SUGINO Yosuke, ITO Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 114th OR-29 - OR-29 2021

Examination of the mechanism of the reaction to increase oviductal tonus by Tokishakuyakusan

KUBOTA Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 114 OR-28 - OR-28 2021

Involvement of gap junction in the increased contraction of bovine oviductal isthmus before ovulation

YAMAMOTO Yuki, KUBOTA Sayaka, KIMURA Koji

The Journal of Reproduction and Development Supplement 114 OR-27 - OR-27 2021

Investigation of sperm chemotactic factor expression in bovine endometrium

MUNETOMO Maho, SAKAI Syunsuke, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 66 ( Suppl. ) j78 - j78 2020.9

ウシ子宮内膜side population(SP)細胞の遺伝子発現解析と分娩後におけるSP細胞割合の推移

舘林亮輝, 中村翔, 美辺詩織, 古澤軌, 阿部良哉, 加治佐実希, 森田康広, 大蔵聡, 木村康二, 松山秀一

The Journal of Reproduction and Development 66 ( Suppl. ) j41 - j41 2020.9

The effect of Toki-syakuyaku-san on bovine oviduct peristaltic movement

KUBOTA Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 66 ( Suppl. ) j75 - j75 2020.9

Analysis of the pattern of intracellualr Ca²⁺ dynamics in cultured bovine oviductal smooth muscle cells

YAMAMOTO Yuki, ISHIMOTO Kenta, KIMURA Koji

The Journal of Reproduction and Development Supplement 66 ( Suppl. ) j76 - j76 2020.9

Evaluation of cell aggregations and cysts of bovine oviductal epithelial cells in 3D culture

SUGINO Yosuke, ITO Sayaka, SATO Taiki, SAKAI Syunsuke, UMEBARA Ibuki, KUBOTA Sayaka, MUNETOMO Maho, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 66 ( Suppl. ) j76 - j76 2020.9

Bovine endometrial stromal cells increase IL-6 expression via endoplasmic reticulum response in heat stress conditions

SAKAI Shunsuke, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 66 ( Suppl. ) j41 - j41 2020.9

ウシの発情周期中における卵管自発収縮の制御機構

山本ゆき, 山本ゆき, 小川泰司, 黒川真帆, 窪田早耶香, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020

暑熱ストレスは小胞体ストレスを介して子宮内膜間質細胞のIL6発現を増加させる

酒井駿介, 山本ゆき, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020

Pregnancy-specific expression of IFIT1 in the bovine external uterine mucosa

ASAOKA Natsuki, KIMURA Koji, TAKAHASHI Masashi, KUNII Hiroki, KOYAMA Keisuke, KUBO Tomoaki, HAMAGUCHI Yu, OGAWA Hidehiko, KOBAYASHI Hisato, BAI Hanako, KAWAHARA Manabu

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j129 - j129 2019.9

【Female genital tractの収縮を再考する】卵管の平滑筋収縮

木村康二

HORMONE FRONTIER IN GYNECOLOGY 26 ( 3 ) 185 - 189 2019.9

Language：Japanese Publisher：(株)メディカルレビュー社

卵管の平滑筋の運動は配偶子や胚の輸送を担う重要な機能の1つである。卵管の平滑筋は自律的な収縮弛緩による蠕動運動を行うことで輸送機能は果たしている。消化管と同じくある種のペースメーカー細胞の存在が示唆されているが、その詳細はいまだ解明されていない。また、卵管の平滑筋による輸送機能は性周期に依存して変化しており、これは卵巣由来の性ステロイドホルモンの直接または間接的な影響によるものと考えられている。(著者抄録)

DOI： 10.34449/j0015.26.03_0029-0033

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Progress of ciliogenesis in bovine oviductal epithelium preovulatory phase of the estrous cycle

ITO Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j83 - j83 2019.9

The effect of heat stress on macrophage chemotaxis in bovine endometrial cells

SAKAI Shunsuke, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j128 - j128 2019.9

Expression analysis of stem cell markers in bovine endometrial side population cells

TATEBAYASHI Ryoki, NAKAMURA Sho, MINABE Shiori, FURUSAWA Tadashi, ABE Ryoya, MORITA Yasuhiro, OHKURA Satoshi, KIMURA Koji, MATSUYAMA Shuichi

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j133 - j133 2019.9

Mechanism for regulation of calcium oscillation in cultured bovine oviductal smooth muscle cells

YAMAMOTO Yuki, KUROKAWA Maho, KIMURA Koji

The Journal of Reproduction and Development Supplement 65 ( Suppl. ) j134 - j134 2019.9

ウシ主席卵胞および次席卵胞の顆粒層細胞において発現する遺伝子の網羅的解析

大下雪奈, 渡辺雄貴, 春日崇, 中平陽子, 棟朝亜理紗, 木村康二, 松山秀一, 大蔵聡, 真方文絵, 松田二子

日本獣医学会学術集会講演要旨集 162回 439 - 439 2019.8

Survey of active site of interferon-tau by treating chemically synthetic peptides in endometrium cells

SUZUKI Toshiyuki, KIMURA Koji, BAI Hanako, KAWAHARA Manabu, TAKAHASHI Masashi

Hokkaido Journal of Livestock and Grassland Science 7 ( 1 ) 7 - 15 2019

Language：English Publisher：Hokkaido Society of Livestock and Grassland Science

<p>Interferon-tau (IFN-τ:195 aa) is a unique protein secreted only from ruminant and plays a very important role of pregnancy recognition. However, the source of IFN-τ is recombinant IFN-τ using E.coli expression system and it is very difficult to obtain. Therefore basic research and application research for improving pregnancy is highly limited. Besides, use of recombinant IFN-τ for field application is strictly regulated by biosafety and public consensus. Therefore, the purpose of this research is to discover the active site of IFN-τ by using of synthetic peptides as a substitute for recombinant IFN-τ. Eleven peptides (long chain: 27-28 aa, short chain: 7-17 aa) were chemically synthesized from amino acid sequence of IFN-τ. To confirm the agonistic activity of the synthetic peptides, single or mixed peptides were added to cultured bovine endometrium stromal cells to detect gene expression of interferonstimulated genes (ISGs). Recombinant IFN-τ was also added as a positive control. Next experiment was performed to confirm the antagonistic activity of the synthetic peptides. Single or mixed peptides was added to stromal cells with recombinant IFN-τ followed by detecting of ISGs expression. IFN-τ significantly stimulated ISGs expression, whereas all peptides did not stimulate. Besides, addition of single or mixed peptides with IFN-τ showed no inhibitory effect. Overall results suggest that synthetic peptides would not have sufficient agonistic and antagonistic ability or lost the binding ability to IFN receptor by the possible structural change or no activity site having.</p>

DOI： 10.32253/hlgs.7.1_7

ウマ子宮内膜におけるPGF2α自己増幅機構

香西圭輔, 徳山翔太, Szostek Anna Z, 登石裕子, 角田修男, 田谷一善, 阪谷美樹, 高橋昌志, 南保泰雄, Skarzynski Dariusz J, 山本ゆき, 木村康二, 奥田潔

馬の科学 = Equine science 56 ( 4 ) 282 - 293 2019

暑熱環境下におけるウシ子宮内膜細胞のマクロファージ走化性物質産生の変化

酒井駿介, 山本ゆき, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

卵管自発収縮に関わるイオンチャネルと培養平滑筋細胞内カルシウム変動

山本ゆき, 山本ゆき, 黒川真帆, 小川泰司, 木村康二, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

着床前ウシ頸管および外子宮口周辺粘膜におけるインターフェロン誘導遺伝子の発現

国井宏樹, 伊藤月乃, 小木曽貴季, 鈴木惇文, 古山敬祐, Md. Abdus, Shabur Talkder, Balboula Ahmed Zaky, 唄花子, 川原学, 永野昌志, 木村康二, 高橋昌志

北海道牛受精卵移植研究会会報 37 17 - 17 2018.8

ウシ黄体におけるbetaglycanの役割とその発現調節

長谷川啓喜, 山下真路, 伊藤典彦, 岡本芳晴, 窪友瑛, 伊賀浩輔, 木村康二, 菱沼貢, 奥田潔, 西村亮, 西村亮

日本畜産学会大会講演要旨 124th 205 - 205 2018.3

ウシ伸長期胚の非外科的移植技術—Non-surgical Transfer Method of Elongating Bovine Conceptus

木村, 康二

岡山大学農学部学術報告 107 19 - 24 2018.2

Language：Japanese Publisher：岡山大学農学部

The objectives of the present study were to establish a non-surgical transfer method for elongating bovine conceptuses and to combine this method with biopsy and sexing. Bovine conceptuses were recovered from donor cows on day 13-14 of the estrus cycle. In experiment 1, day 13 conceptuses were transferred to recipient cows using a standard day 7 embryo transfer (ET) method. The pregnancy rate of day 13 conceptus transfer (CT) is comparable to that of day 7 ET. In experiment 2, day 14 conceptuses were transferred using modified methods (balloon catheters or ET guns with modified sheaths). Using the standard ET method, no pregnancies were obtained;however, when balloon catheters or ET guns with modified sheaths were used, the pregnancy rates after CT were 48.0％ and 44.8％, respectively. In experiment 3, day 14 conceptuses were biopsied without a micromanipulator, sexed using the loop-mediated isothermal amplification (LAMP) method, and transferred to recipient cows. The pregnancy rate of biopsied conceptuses was 46.2％ and did not differ significantly from that of unbiopsied with conceptuses. Moreover, all pregnant cows transferred conceptuses following biopsy and sexing delivered calves with the expected sexes. These results suggested that the non-surgical bovine conceptus transfer (CT) method was comparable to day 7 ET, and that this technique enables biopsy and sexing without expensive equipment such as a micromanipulator or specialized skills.

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Effect of heat stress on IFNT responsiveness in bovine uterine epithelial cells

KOMATSU Seimei, SUZUKI Toshiyuki, KUNII Hiroki, KAWAHARA Manabu, KIMURA Koji, TAKAHASHI Masashi, BAI Hanako

The Journal of Reproduction and Development Supplement 111 ( 0 ) P - 38-P-38 2018

TRP channels are localized on bovine endometrium and involved in increase of prostaglandin production of bovine endometrial stromal cells under heat stress

YAMADA Ai, TAKAMI Keito, SAKAI Shunsuke, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 64 ( Suppl Japanese Issue ) P-39 - P-39 2018

Evaluation of the ion channels involved in phasic spontaneous contraction and intracellular calcium signaling in cultured smooth muscle cells of bovine oviducts

YAMAMOTO Yuki, KUROKAWA Maho, OGAWA Taiji, KIMURA Koji

The Journal of Reproduction and Development Supplement 64 ( Suppl Japanese Issue ) OR2-14 - OR2-14 2018

Estimation of differentiation pathway by charactarization of bovine oviductal epithelial cells

ITO Sayaka, YAMAMOTO Yuki, KIMURA Koji

The Journal of Reproduction and Development Supplement 64 ( Suppl Japanese Issue ) P-40 - P-40 2018

ウシ卵管上皮にみられる段階的な繊毛形成過程

伊藤さやか, 山本ゆき, 木村康二

日本分子生物学会年会プログラム・要旨集(Web) 41st 2018

Relationship between LH sensitivity and collagen structure in bovine luteal cells

MARUO China, HASHIBA Kazuhisa, WATANABE Daisuke, KIMURA Koji

The Journal of Reproduction and Development Supplement 111 OR2-22 - OR2-22 2018

ウシ初期黄体における低酸素環境のglucose transporter 1(GLUT1)発現を介した機能亢進

西村亮, 西村亮, 長谷川啓喜, 山下真路, 伊藤典彦, 岡本芳晴, 竹内崇, 窪友瑛, 伊賀浩輔, 木村康二, 菱沼貢, 奥田潔, 奥田潔

日本獣医学会学術集会講演要旨集 160th 431 - 431 2017.8

ウシ初期および中期黄体細胞のプロジェステロン(P4)分泌に及ぼす低酸素環境の影響

長谷川啓喜, 西村亮, 菱沼貢, 山下真路, 伊藤典彦, 岡本芳晴, 窪友瑛, 伊賀浩輔, 木村康二, 奥田潔

日本畜産学会大会講演要旨集 122回 160 - 160 2017.3

The effect of interferon tau on human placental cells

Nao Tanikawa, Yugo Suzue, Schuichi Matsuyama, Koji Kimura, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna

JOURNAL OF REPRODUCTIVE IMMUNOLOGY 118 134 - 134 2016.11

トクシュウメザセ、カクジツナコウシセイサン

( 488 ) 34 - 37 2016.11

ウシ伸長胚を利用した現場実施可能な雌雄産み分け技術

松山秀一, 中村翔, 木村康二

畜産技術 = Livestock technology 2016 ( 738 ) 8 - 11 2016.11

Effect of subunit-specific knockdown of IFNAR on expression of IFNτ signal-related genes

SHIROZU Takahiro, SUZUKI Toshiyuki, IWANO Hiroki, OGISO Takatoshi, KIM Sung Woo, BAI Hanako, KAWAHARA Manabu, KIMURA Koji, TAKAHASHI Masashi

The Journal of Reproduction and Development Supplement 109 ( 0 ) P - 14-P-14 2016

Examination of preservation methods of bovine elongating conceptus for preimplantation genomic selection

FUJII Takashi, HIRAYAMA Hiroki, KAGEYA Soichi, NAITO Akira, FUKUDA Shigeo, YOSHINO Hitomi, MATSUYAMA Shuiti, KIMURA Koji, HAYAKAWA Hiroyuki, TSUDA Sakae, MORIYASU Satoru

The Journal of Reproduction and Development Supplement 62 ( Suppl Japanese Issue ) P-101 - P-101 2016

ウシ伸長胚を用いた着床前ゲノム選抜技術開発に向けたSNP解析精度の検討

平山博樹, 内藤学, 鹿島聖志, 藤井貴志, 陰山聡一, 坂井ひとみ, 杉本喜憲, 松山秀一, 木村康二, 早川宏之, 森安悟

Journal of Reproduction and Development 62 ( Suppl Japanese Issue ) 2016

Effects of intravenous administration of NKB receptor subtype-selective agonist on LH secretion and embryo quality in superovulated cattle

NAKAMURA Sho, KIMURA Koji, MINABE Shiori, OHISHI Shinya, OHKURA Satoshi, MATSUYAMA Shuichi

The Journal of Reproduction and Development Supplement 62 ( Suppl Japanese Issue ) OR2-27 - OR2-27 2016

ウシの子宮機能と受胎性について—Relationship between uterine function and establishment of pregnancy in cattle

木村康二, 松山秀一

家畜感染症学会誌 = The journal of farm animal in infectious disease 5 ( 4 ) 145 - 151 2016

Possible role of testosterone in regression of bovine corpus luteum

IRIE Yui, HASHIBA Kazuhisa, KIMURA Koji, OKUDA Kiyoshi

The Journal of Reproduction and Development Supplement 109 OR2-34 - OR2-34 2016

Role of core fucosylation on epidermal growth factor receptor in bovine luteal function

HASHIBA Kazuhisa, KOBAYASHI Junko, SANO Masahiro, MAEDA Megumi, KIMURA Yoshinobu, OKUDA Kiyoshi, KIMURA Koji

The Journal of Reproduction and Development Supplement 109 OR2-33 - OR2-33 2016

Effect of heat stress (HS) on luteolytic mechanism in bovine endometrial cells

SAKAI Shunsuke, YAGI Mami, KUSE Mariko, YAMAMOTO Yuki, KIMURA Koji, OKUDA Kiyoshi

The Journal of Reproduction and Development Supplement 109 OR1-2 - OR1-2 2016

ウシ胚におけるインターフェロンτの局在

細江実佐, 林憲悟, 作本亮介, 古澤軌, 池田光美, 大越勝広, 徳永智之, 木村康二, 松山秀一, 野口純子, 高橋透

日本畜産学会大会講演要旨 119th 204 2015.3

ウシ過剰排卵処置時におけるウシ型キスペプチン‐10の静脈内への頻回投与が性腺刺激ホルモン分泌および排卵成績に及ぼす影響

MATSUYAMA SHUICHI, OKURA SATOSHI, OISHI SHIN'YA, KIMURA KOJI

日本はい移植学雑誌 37 ( 1 ) 34 2015.1

Gene expressions of enzymes involved in testosterone synthesis in the bovine corpus luteum

IRIE Yui, HASHIBA Kazuhisa, YOSHIOKA Shin, KIMURA Koji, YAMAMOTO Yuki, OKUDA Kiyoshi

The Journal of Reproduction and Development Supplement 61 ( Suppl Japanese Issue ) P-36 - P-36 2015

ウシ卵管における細胞模型エストロジェン受容体(GPER)を介したestradiol-17β(E₂)の作用

西江拓海, 小林芳彦, 山本ゆき, 木村康二, 奥田潔, 奥田潔

日本畜産学会大会講演要旨 119th 2015

The changing of glucocorticoid effector in the equine endometrium throughout the estrous cycle

SHIOTANI Haruki, ANNA Z Szostek, YAMAMOTO Yuki, KIMURA Koji, OKUDA Kiyoshi

The Journal of Reproduction and Development Supplement 108 P-62 - P-62 2015

piggyBac トランスホソンを用いたリフログラミンク因子導入によるウシ羊膜細胞からの栄養膜細胞株樹立の試み

川口高正, 築山智之, 松山秀一, 木村康二, 南直治郎, 山田雅保, 今井裕

第107回日本繁殖生物学会大会 107 P-20 - P-20 2014.8

Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：公益社団法人日本繁殖生物学会

【目的】栄養膜細胞は胚発生で起こる最初の細胞分化により作り出される細胞であり，着床や胎盤形成に重要な役割を担っている。マウスやヒトでは着床初期の研究は盛んに行われているが，ウシでは胚発生および着床・妊娠プロセスは大きく異なっていることもあり，未だ不明瞭な部分も多い。さらに，ウシではこれらの研究を進める上で有用と思われる，栄養膜幹細胞株の報告はなく，それから派生する細胞系譜の細胞株の種類も少ない。本研究では，ウシの羊膜細胞から人工栄養膜細胞(induced trophoblast cells; iTB細胞)の樹立を試みた。【方法】妊娠50日齢の胎仔から採取した羊膜細胞に，Oct3/4，Sox2，Klf4およびc−Mycの転写因子を，piggyBacトランスポゾンベクターを用いて導入した。このベクターには，ドキシサイクリン（Dox）添加の有無によって，導入した転写因子の発現が制御できるシステムを組み込んだ。Doxの添加から4日後，遺伝子導入後の細胞をフィーダー細胞上へと移し，10%のFBSを含む培地で培養を行った。【結果】Doxの添加から2週間後，上皮細胞様で敷石状の形態を示すコロニーが出現した。このコロニーは，その細胞形態を維持したまま40回以上継代を行うことができた。得られた細胞株は，幹細胞マーカーであるアルカリフォスファターゼ活性を部分的に示していた。また，栄養膜細胞関連遺伝子（CDX2，ESRRβ，IFNτ）を発現していた。さらに，低接着ディッシュ上で数日間浮遊培養し，分化を促すと栄養膜小胞(Trophoblastic vesicle)様の胞状球体および二核細胞の形成が認められた。これらの結果から，羊膜細胞にリプログラミング因子を発現させることにより，人工的に栄養膜細胞株を得ることが可能になった。得られた細胞株は，ウシの着床・妊娠プロセスにおける栄養膜細胞の機能や細胞分化の研究のための有用なツールとなると考えられる。

DOI： 10.14882/jrds.107.0_p-20

CiNii Article

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インターフェロン(IFN)τはナノ粒子の取り込みを阻害してインターロイキン(IL)-1β産生を抑制する

原教子, 白砂孔明, 臼井文武, 唐澤直義, 木村博昭, 川島晃, 大口昭英, 松山秀一, 木村康二, 高橋将文

The Journal of Reproduction and Development 60 ( Suppl. ) j137 - j137 2014.8

ウシ子宮内膜組織におけるMX1とMX1B発現に及ぼすI型インターフェロンの応答性およびその受容体の発情周期別比較

白水貴大, 川原学, 木村康二, 高橋ひとみ, 柳川洋二郎, 永野昌志, 白汝嵐, 今川和彦, 高橋昌志

J Reprod Dev 107 ( 0 ) OR2 - 24-OR2-24 2014

ウシ子宮内膜上皮細胞におけるSide populationの遺伝子発現解析

松山秀一, 古澤軌, 池田光美, 木村康二

日本繁殖生物学会講演要旨集 107 P-69 - P-69 2014

Language：Japanese Publisher：公益社団法人日本繁殖生物学会

【目的】ヒトの子宮内膜は，エストロジェンやプロジェステロンの支配を受けて月経周期ごとに脱落と増殖を繰り返し，絶えず更新される。さらに，子宮に存在する組織幹細胞（子宮内膜幹細胞）が子宮内膜の更新に寄与することが示唆されているが，ヒトとは異なる子宮内膜更新メカニズムを有するウシにおいては，子宮における幹細胞の存在について未だ明らかでない。本研究では，ウシの子宮内膜更新メカニズムを明らかにする第一歩として，ウシ子宮における組織幹細胞の存在を明らかにすることを目的とした。【方法および結果】未経産牛の子宮からトリプシン処理により単離し，培養した子宮内膜上皮細胞を用いてHoechst33342にて染色後，フローサイトメトリー解析に供した。その結果，ウシ子宮内膜上皮培養細胞には蛍光強度の低い領域に突出した細胞集団が存在し，この細胞集団はATP binding cassette（ABC）トランスポーターの阻害剤であるベラパミル存在下で消失した。このことから，ヒト等において幹細胞活性を有すると報告されているSide population (SP) 細胞がウシの子宮内膜上皮細胞においても存在することが明らかとなった。さらにセルソーターによって，ウシの子宮内膜上皮培養細胞からSP細胞とそれ以外の細胞（Major population, MP細胞）を分取し，それぞれの細胞における遺伝子発現についてDNAマイクロアレイ法により解析した。その結果，ウシ子宮内膜上皮細胞におけるSP細胞では組織・上皮増殖や細胞接着，免疫機能に関わる遺伝子の発現量が高いことが明らかとなった。

DOI： 10.14882/jrds.107.0_p-69

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Generation of murine-ES-cell-like iPS cells in cattle using piggyBac transposition of doxycycline-inducible transcription factor Reviewed

T. Kawaguchi, T. Tsukiyama, N. Minami, M. Yamada, K. Kimura, H. Imai

CiRA International Symposium 2013 ~Raising the next generation of stem cell research~, Kyoto, Japan 2013.3

ウシにおけるマウスES細胞様iPS細胞の樹立の試み Reviewed

川口高正, 南直治郎, 山田雅保, 木村康二, 今井裕

第1回関西生殖医学集談会 2013.3

ウシ過剰排卵処置時におけるウシ型キスペプチン‐10の単回末梢投与が性腺刺激ホルモン分泌および卵巣機能に及ぼす影響

MATSUYAMA SHUICHI, OKURA SATOSHI, OISHI SHIN'YA, KIMURA KOJI

東日本家畜受精卵移植技術研究会大会資料 29th 39 2013

Transdifferentiation of bovine aminion cells into trophoblast-like cells using piggyBac transposition of doxycycline-inducible reprogramming factors Reviewed

T. Kawaguchi, T. Tsukiyama, N. Minami, M. Yamada, K. Kimura, H. Imai

Satellite symposium to the International Congress on Animal Reproduction (ICAR) 2012.7

Effect of maternal ageing on the glutathione content and p38 MAPK activity in bovine granulosa cells

TAKEO Shun, GOTO Hiroya, KIMURA Koji, IWATA Hisataka, MONJI Yasunori, KUWAYAMA Takehito

29 ( 2 ) S60 2012.4

Sexual dimorphism during early embryonic development in mammals

Koji Kimura, Shuichi Matsuyama

Journal of Mammalian Ova Research 29 ( 3 ) 103 - 112 2012

Language：English Publishing type：Book review, literature introduction, etc.

Although most sexual dimorphism appears after gonadal differentiation, some occur at earlier stages of development. One example of this phenomenon is the skewing of the sex ratio of embryos due to the glucose concentration of the culture medium. This skewing occurs because male and female embryos differ in their abilities to metabolize glucose. Another example is the sexually dimorphic expression of the bovine embryo-derived signal for maternal recognition of pregnancy, IFNT. The difference in expression of X-linked genes between male and female embryos, that results from incomplete X-chromosome inactivation, is considered to be involved, either directly or indirectly, in the sexual dimorphism that appears during early development. © 2012 Japanese Society of Mammalian Ova Research.

DOI： 10.1274/jmor.29.103

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CHANGES IN PLASMA STEROID CONCENTRATIONS DURING GESTATION IN COWS WITH SPONTANEOUS ABORTION OF SOMATIC CELL CLONED FETUSES

M. Hirako, H. Takahashi, K. Kimura, N. Adachi, S. Akagi

REPRODUCTION FERTILITY AND DEVELOPMENT 24 ( 1 ) 121 - 121 2012

Oog1 promoter controls the meiosis-specific gene expression in germ cells Reviewed

Ishida M, Okazaki E, Tsukamoto S, Kimura K, Ohota Y, Kito S, Imai H, Minami N

The 45th Annual Meeting of the Society for the Study of Reproduction 2012

Establishment of induced pluripotent stem cells in cattle using piggyBac transposition of doxycycline-inducible transcription factors

T. Kawaguchi, T. Tsukiyama, K. Kimura, N. Minami, M. Yamada, H. Imai

World Congress on Reproductive Biology (WCRB), Cairns, Australia 2011.10

Identification of Oog1 Promoter Regions in Mouse Growing Oocytes

Miya Ishida, Eriko Okazaki, Satoshi Tsukamoto, Koji Kimura, Yuki Ohta, Seiji Kito, Hiroshi Imai, Naojiro Minami

BIOLOGY OF REPRODUCTION 85 2011.7

The Possible Involvement of IFN-Tau in Lymphangiogenesis in the Corpus Luteum During Maternal Recognition Period in the Cow

Akane Nitta, Koumei Shirasuna, Haruka Matsumoto, Shingo Haneda, Motozumi Matsui, Takashi Shimizu, Shuichi Matsuyama, Koji Kimura, Heinrich Bollwein, Akio Miyamoto

BIOLOGY OF REPRODUCTION 85 225 - 225 2011.7

ウシにおける人工多能性幹細胞の樹立の試み

川口高正, 築山智之, 木村康二, 南直治郎, 山田雅保, 今井裕

第136回日本生殖医学会関西支部集談会、ハービスプラザ、大阪 2011.3

The relationship among number of conceptuses, elongation of conceptuses and plasma progesterone concentrations in superovulated cattle

MATSUYAMA Shuichi, SAKAGUCHI Yosuke, KIMURA Koji

The Journal of Reproduction and Development Supplement 104 1075 - 1075 2011

Promoter analysis of oocyte-specific gene, oog1

ISHIDA Miya, OKAZAKI Eriko, TSUKAMOTO Satoshi, KIMURA Koji, OHTA Yuki, KITO Seiji, IMAI Hiroshi, MINAMI Naojiro

The Journal of Reproduction and Development Supplement 104 1004 - 1004 2011

Identification of Oog1 Promoter Regions which Function During the First Meiosis in Oocytes. Reviewed

Eriko Okazaki, Satoshi Tsukamoto, Koji Kimura, Yuki Ohta, Seiji Kito, Hiroshi Imai, Naojiro Minami

BIOLOGY OF REPRODUCTION 170 - 170 2010

The Relationship Between Pregnancy Status and Production of IFN-Tau of Bovine Conceptus by Measuring Expression of Interferon- Stimulated Gene 15 (ISG15) of Peripheral Leukocyte in Cattle

Shuichi Matsuyama, Takatoshi Kojima, Satoshi Kato, Shogo Shiratsuki, Hiroshi Tanaka, Keiichi Nagataki, Shoichi Hoshi, Koichi Muroi, Koji Kimura

BIOLOGY OF REPRODUCTION 152 - 152 2010

RELATIONSHIP BETWEEN FETAL ABNORMALITIES AND PERIPHERAL STEROID CONCENTRATIONS DURING GESTATION IN COWS TRANSFERRED WITH EMBRYOS PRODUCED BY SOMATIC CELL NUCLEAR TRANSFER

M. Hirako, H. Takahashi, K. Kimura, N. Adachi, S. Akagi

REPRODUCTION FERTILITY AND DEVELOPMENT 22 ( 1 ) 187 - 187 2010

Effect of corpus luteum on sperm storage ability of oviduct epithelial cells and concentration of progesterone in bovine oviducts.

SAKAGUCHI Yosuke, Omura Manami, MATSUYAMA Shuichi, IWATA Hisataka, KIMURA Koji, MONJI Yasunori, KUWAYAMA Takehito

The Journal of Reproduction and Development Supplement 103 121 - 121 2010

Renewal of techniques related to embryo transfer in cattle : a novel superovulation method using aluminum hydroxide gel

KIMURA Koji, MATSUYAMA Shuichi, IWATA Hisataka, SEKI Makoto, HIRAKO Makoto

The Journal of reproduction and development 55 j60 - j61 2009.8

ZP3 promoter is not enough to analyze the early expressing genes during oogenesis, such as Oog1 Analysis of Oog1, an oocyte-specific gene, using transgenic RNAi approach. Reviewed

Imaichi H, Okazaki E, Tsukamoto S, Ohota Y, Kito S, Kimura K, Imai H, Minami N

42nd Society for the Study of Reproduction 2009

ZP3 Promoter Is Not Enough to Analyze the Early Expressing Genes During Oogenesis, Such as Oog1

Hisashi Imaichi, Rieko Okazaki, Satoshi Tsukamoto, Yuki Ohta, Seiji Kito, Koji Kimura, Hiroshi Imai, Naojiro Minami

BIOLOGY OF REPRODUCTION 128 - 128 2009

Relation between IFN-tau secretion and conception in cattle

MATSUYAMA Shuichi, KOJIMA Takatoshi, KATO Satoshi, SHIRATSUKI Shogo, TANAKA Hiroshi, NAGATAKI Keiichi, HOSHI Shoichi, MUROI Koichi, KIMURA Koji

The Journal of Reproduction and Development Supplement 102 1086 - 1086 2009

Oog1 is a maternal effect gene required for the development of mouse preimplantation embryo Reviewed

N. Minami, H. Imaichi, S. Tsukamoto, Y. Ohta, S. Kito, K. Kimura, H. Imai

REPRODUCTION IN DOMESTIC ANIMALS 43 149 - 150 2008.7

Effect of FCS and BSA on hatching rate, apoptosis, and stress activated protein kinase activity in bovine blastocysts

Hisataka Iwata, Yosuke Sakaguchi, Yoshihiro Kon, Koji Kimura, Takehito Kuwayama, Yasuniri Monji

BIOLOGY OF REPRODUCTION 94 - 94 2008

Analysis of Oog1, an oocyte-specific gene, using transgenic RNAi approach

Hisashi Imaichi, Satoshi Tsukamoto, Yuki Ohta, Seiji Kito, Koji Kimura, Hiroshi Imai, Naojiro Minami

BIOLOGY OF REPRODUCTION 133 - 133 2008

黒毛和種のワンショット過剰排卵処理法の確立に関する研究(2) : 筋肉内投与法におけるホルモン量および試作ゲルの有効性の検討

林, 登, 星野, 洋一郎, 加藤, 誠二, 坂口, 慎一, 三品, 賢三, 関, 誠, 竹内, 昭司, 角川, 博哉, 木村, 康二

岐阜県畜産研究所研究報告 ( 7 ) 21 - 25 2007.7

Language：Japanese

黒毛和種における水酸化アルミニウムゲルを用いた卵胞刺激ホルモン（FSH）の1回投与法（ワンショット法）による過剰排卵処理法について、FSHの投与量及び試作水酸化アルミニウムゲルの実用性を検討した。試験1として、ワンショット法におけるホルモンの投与量を検討するため、黒毛和種経産牛にFSH30AU（n＝15）あるいは25AU（n＝13）を筋肉内投与する過剰排卵処理を実施し、採卵成績として採卵時の黄体数、大卵胞数、採卵数および正常胚数を調査し比較した。また試験2として、従来の市販水酸化アルミニウムゲル（Sigma社）に替えて、川崎三鷹製薬株式会社で開発された試作水酸化アルミニウムゲルを用いたワンショット法（FSH25AU筋肉内投与）と漸減投与法（FSH5、5、3、3、2、2計20AU筋肉内投与）による過剰排卵処理を、黒毛和種経産牛8頭に各1回ずつ実施し採卵成績を比較した。1．試験1における採卵時の平均黄体数と大卵胞数は、30AU区でそれぞれ7.0±4.8個と1.7±1.0個、25AU区でそれぞれ7.2±6.4個と1.0±0.8個であり、いずれにおいても両区間に有意な差は認められなかった。また、採卵個数と正常胚数についても、30AU区でそれぞれ5.2±4.7個と3.5±4.2個、25AU区でそれぞれ5.8±6.0個と4.8±5.4個であり、いずれにおいても両区間に有意な差は認められなかった。しかし、胚の品質は25AU区でより優れていた。2．試験2における採卵時の平均黄体数と大卵胞数は、ワンショット区でそれぞれ7.9±6.6個と3.6±4.8個、漸減投与区でそれぞれ9.4±6.3個と5.1±5.4個であり、いずれにおいても両区間に有意な差は認められなかった。また、採卵個数と正常胚数も、ワンショット区でそれぞれ6.1±5.8個と4.5±4.4個、漸減投与区でそれぞれ6.0±3.5個、2.6±3.0個であり、いずれにおいても両区間に有意な差は認められなかった。しかし、胚の品質ではワンショット区でより良い結果が得られた。以上の結果から、黒毛和種における水酸化アルミニウムゲルを用いたFSHの1回投与による過剰排卵処理では、FSHの投与量を25AUまで減量できることが示唆された。また新たに開発された水酸化アルミニウムゲルも従来のゲルの代わりに用いることができることが明らかとなった。

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A comparison of pregnancy maintenance after embryo transfer of Day 7 or 13 bovine embryos and the relationship between interferon-tau production of day 13 bovine embryos and pregnancy success

K. Kimura, N. Hayashi, H. Iwata

REPRODUCTION FERTILITY AND DEVELOPMENT 19 ( 1 ) 222 - 223 2007

第3章流・死産の発生要因の解明(4)妊娠末期のクローン胎子の発育および分娩発来機構と正常分娩誘起法の開発に関する研究

平子誠, 平子誠, 青木真理, 青木真理, 木村康二, 木村康二, 花房泰子, 石崎宏, 假屋喜弘

農林水産省農林水産技術会議事務局研究成果 ( 444 ) 2007

Culture condition for bovine blastocyst

Sasaki Yasuhito, Kon Yoshihiro, Sakaguchi Yosuke, Yamamoto Taiyo, Kimura Koji, Iwata Hisataka, Kuwayama Takehito, Monji Yasunori

The Journal of Reproduction and Development Supplement 100 20077 - 20077 2007

Expression of bovine interferon-tau variants according to sex and age of conceptuses.

Angela M. Walker, Koji Kimura, R. M. Roberts

BIOLOGY OF REPRODUCTION 93 - 93 2006

The effect of glucosamine concentration on the development and sex ratio of bovine embryos.

Koji Kimura, Hisataka Iwata, Jeremy G. Thompson

BIOLOGY OF REPRODUCTION 124 - 124 2006

Mechanisms for Establishment of Pregnancy in Mammalian Species

KIMURA Koji

Journal of mammalian ova research 22 ( 3 ) 101 - 118 2005.10

A compariosn of IFN-tau messenger RNA expression between somatic nuclear transfer and in vitro produced female bovine blastocysts.

K Kimura, S Akagi, S Takahashi, H Iwata, H Kadokawa, M Aoki, M Hirako

BIOLOGY OF REPRODUCTION 230 - 230 2005

水酸化アルミニウムゲルを用いたFSH1回投与によるウシ過剰排卵誘起法

木村康二

畜産技術 = Livestock technology ( 590 ) 2 - 4 2004.7

Successful superovulation of cattle using a single intramuscular injection of FSH with aluminum hydroxide gel.

K Kimura, M Hirako, H Iwata, M Aoki

BIOLOGY OF REPRODUCTION 237 - 238 2004

Responsiveness of rabbits to superovulation treatment by single injection of follicle-stimulating hormone mixed with aluminum hydroxide gel.

S Hashimoto, K Kimura, T Kuramochi, K Aoyagi, M Hirako, M Kawaguchi, H Iwata, H Takahashi, M Hirao, K Kitada, K Hirasawa, M Ueda

BIOLOGY OF REPRODUCTION 236 - 237 2004

Effect of oxidative stress and inhibitors of the pentose-phosphate pathway on the sexual dimorphic expression of IFN-tau production by bovine blastocysts

K Kimura, LD Spate, RM Roberts

BIOLOGY OF REPRODUCTION 68 158 - 158 2003

The effects of high versus low oxygen and of the presence of glucose and fructose during culture on the sex ratio of bovine blastocysts.

RM Roberts, MP Green, LD Spate, K Kimura

BIOLOGY OF REPRODUCTION 68 250 - 251 2003

Cannulation for a bovine fetus in late gestation under regional anesthesia

M Aoki, K Kimura, M Hirako, Y Hanafusa, H Ishizaki, Y Kariya

JOURNAL OF REPRODUCTION AND DEVELOPMENT 48 ( 5 ) 455 - 460 2002.10

Language：English Publisher：SOCIETY REPRODUCTION & DEVELOPMENT-SRD

This experiment was carried out to collect fetal blood and fluid via catheters fitted to elucidate interaction between the mother and her fetus during a comparatively long term. Fifteen cows in late gestation were used for this study. Under regional anesthesia, an incision was made in the left paralumbar fossa in the standing position. A catheter for fetal blood was inserted into a vein of the fetus and catheters for fetal fluid were inserted into amniotic and allantoic sacs respectively. Four types of catheters were used for collecting fetal blood. The best of these catheters for fetal vein was the angiographic catheter covered with spring tube. Fetal blood samples were able to be collected for more than seven days constantly from four fetuses until parturition. Fetal fluid could be collected daily from three fetuses until parturition perfectly. Parturition occurred at the end of a normal gestation period. The concentration of fetal and maternal cortisol decreased to the basal level within 24 hours (P<0.05) after operation. The changes in fetal and maternal cortisol during periparturition were the same as previously reported. These results suggested that this method would be beneficial for fetal cannulation and the sample collected by this method was useful for endocrine study during the perinatal period in cattle.

DOI： 10.1262/jrd.48.455

Nursing ability of crossbred (Japanese Black X Holstein) cows on pasture and the effect of supplemental nutrition upon the twin calves and their dam

1 ( 1 ) 1 - 6 2002.3

Female, in vivo-produced bovine blastocysts produce over twice the amount of interferon-tau as males at the same stage of development.

K Kimura, RM Roberts, GE Seidle

BIOLOGY OF REPRODUCTION 66 154 - 155 2002

妊娠末期に牛胎子から血液および羊水・尿膜水を連続的に採取できる

青木真理, 木村康二, 平子誠, 花房泰子, 石崎宏, 仮屋喜弘

畜産草地研究成果情報 ( 1 ) 2002

In vitro cultured male bovine embryos make the transition from morula to blastocyst more successfully than females.

K Kimura, MA Larson, RM Roberts

BIOLOGY OF REPRODUCTION 64 178 - 178 2001

娠妊末期における母ウシおよび胎子の血球数および血液生化学値の動態

花房泰子, 青木真理, 木村康二, 平子誠, 須藤まどか, 石崎宏, 仮屋喜弘

日本獣医学会学術集会講演要旨集 132nd 2001

Effect of Vascular Endothelial Growth Factor on the Early Development of IVM/IVF Bovine Embryos

LUO Hailing, KIMURA Koji, AOKI Mari, HIRAKO Makoto, ZHAO Youzhang

17 ( 2 ) S51 2000.4

Effect of bovine interferon tau on the early development of bovine embryos.

K Kimura, H Iwata, M Aoki, M Hirako

BIOLOGY OF REPRODUCTION 62 250 - 250 2000

妊娠末期における母ウシおよび胎子の血球数動態

花房泰子, 青木真理, 木村康二, 平子誠, 石崎宏, 仮屋喜弘

関東畜産学会大会講演要旨集 55th 2000

放牧における発情牛の運動量の解析(第2報) : ランダムな間隔で測定した場合の解析方法の検討

梅田直円, 加茂幹男, 青木真理, 木村康二, 山田知哉

日本家畜管理学会誌 = Japanese journal of livestock management 35 ( supplement ) 32 - 33 1999.3

ウシ胎子へのカテーテル装着と胎子血採取の試み

青木真理, 木村康二, 平子誠, 石崎宏, 仮屋喜弘, 花房泰子

日本畜産学会大会講演要旨 96th 1999

F1雌牛による双子の安定生産技術

鈴木修, 青木真理, 木村康二

畜産の研究 50 ( 6 ) 675 - 680 1996.6

Preservation of Rat Morula by Vitrification Using Highly Concen-trated Solution of Poly-alcohol

UTSUMI Kyozo, NISHIDA Mieko, KIMURA Kouji, IRITANI Akira

Journal of Reproduction and Development 38 ( 5 ) j7 - j13 1992

Language：English Publisher：The Society for Reproduction and Development

In this study, some characteristics of cryoprotectant, polyalcohol used for vitrification such as toxicity, glass-forming tendency and stability of the amorphous state were estimated and the relationship of those characteristics with cryoprotection for vitrified rat morula was discussed. Glycerol was not harmful for rat morulae exposed for 5 minutes at room temperature to the examined concentration up to 50%. Both of 50% 1, 2-propandiol and 50% 1, 3-butandiol showed detrimental effect on the embryonic survival after exposure for 1 minute. However, combination of 25% propandiol or butandiol with 25% glycerol sustained the survival of embryos at 40-60% even after exposure for 5 minutes. Out of the three cryoprotective solutions, a vitrification solution (VS) of propandiol was easiest to transform into amorphous condition during cooling process. Devitrification (initial) temperature of VS was -92, -77 and -77C for 50% of propandiol, glycerol and butandiol, respectively. Seventy five percent of rat morulae in the solution containing more than 40% glycerol survived when directly plunged into liquid nitrogen, but none of the embryos survived in 50% propandiol or 50% butandiol. Both cryoprotectant, 25% propandiol or 25% butandiol combined with 25% glycerol had a comparable effect with 40-50% glycerol. In 5 percent percoll or 5% PVP supplemented to VS, the survival rate of embryos was improved.

DOI： 10.1262/jrd.38.5_j7

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