研究者詳細 - 前田　恵

論文 - 前田　恵

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Effects of asbestos fibers on human cytotoxic T cells 査読

Naoko Kumagai-Takei, Yasumitsu Nishimura, Hidenori Matsuzaki, Megumi Maeda, Suni Lee, Kei Yoshitome, Takemi Otsuki

Biological Effects of Fibrous and Particulate Substances 211 - 221 2015年10月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Springer Japan

The immunological effects of asbestos have been demonstrated and include reduction of antitumor immunity such as the reduction of natural killer (NK) cell activity with decreased expression of NK cell-activating receptor, NKp46, as well as reduced expression of CXCR3, chemokine receptor, and interferon (IFN)-γ in CD4+ T cells. In this review, the effects of asbestos as demonstrated by our investigations on the cellular characteristics and functions of cytotoxic T lymphocytes (CTLs) differentiated from CD8+ T cells are shown and discussed. Experiments involving in vitro exposure of chrysotile asbestos onto a mixed lymphocyte reaction (MLR) assay to estimate clonal expansion (proliferation and differentiation) of CD8+ T cells revealed the inhibition of both proliferation and differentiation. These results should be interpreted as the impairment of CTL differentiation in the lymph nodes, where asbestos fibers are located continuously in asbestos-exposed people. In addition, analyses of cellular functions in asbestos-exposed patients with pleural plaque (PP) and malignant mesothelioma (MM) are discussed. PP patients showed an increase in effector memory CD8+ T cells compared to healthy donors or MM patients. Furthermore, MM patients showed a decrease in perforin-expressing CD8+ T cells. These results indicated that although asbestos-exposed individuals are ready to respond with transformed cells, CTLs may lose their function once mesothelioma progresses.

DOI： 10.1007/978-4-431-55732-6_12

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beta-Galactosidase from Ginkgo biloba seeds active against beta-galactose-containing N-glycans: purification and characterization 査読国際誌

Md. Ziaur Rahman, Megumi Maeda, Yoshinobu Kimura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 79 ( 9 ) 1464 - 1472 2015年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

In this study, we purified an acidic beta-galactosidase to homogeneity from Ginkgo biloba seeds (beta-Gal'ase Gb-1) with approximately 270-fold purification. A molecular mass of the purified beta-Gal'ase Gb-1 was estimated about 35kDa by gel filtration and 32kDa by SDS-PAGE under non-reducing condition, respectively. On the other hand, beta-Gal'ase Gb-1 produced a single band with a molecular mass of 16kDa by SDS-PAGE under reducing condition. The N-terminal amino acid sequences of 32kDa and 16kDa molecules were the same and identified as H-K-A-N-X-V-T-V-A-F-V-M-T-Q-H-, suggesting that beta-Gal'ase Gb-1 may function as a homodimeric structure in vivo. When complex-type N-glycans containing beta-galactosyl residues were used as substrates, beta-Gal'ase Gb-1 showed substantial activity for beta 1-4 galactosyl residue and modest activity for beta 1-3 galactosyl residue with an optimum pH near 5.0. Based on these results, the involvement of beta-Gal'ase Gb-1 in the degradation of plant complex-type N-glycans is discussed.

DOI： 10.1080/09168451.2015.1034653

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Synthesis of a novel glycopolymer carrying multivalent plant antigenic N-glycans. 査読

Maeda M, Takeda N, Kimura M, Kimura Y

Glycoconjugate Journal 32 ( 5 ) 193 2015年9月

Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing 査読国際誌

Miki Yamamoto-Hino, Hideki Yoshida, Tomomi Ichimiya, Sho Sakamura, Megumi Maeda, Yoshinobu Kimura, Norihiko Sasaki, Kiyoko F. Aoki-Kinoshita, Akiko Kinoshita-Toyoda, Hidenao Toyoda, Ryu Ueda, Shoko Nishihara, Satoshi Goto

Genes to Cells 20 ( 6 ) 521 - 542 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Blackwell Publishing Ltd

Glycan structures are synthesized by a series of reactions conducted by glycosylation-related (GR) proteins such as glycosyltransferases, glycan-modifying enzymes, and nucleotide-sugar transporters. For example, the common core region of glycosaminoglycans (GAGs) is sequentially synthesized by peptide-O-xylosyltransferase, β1,4-galactosyltransferase I, β1,3-galactosyltransferase II, and β1,3-glucuronyltransferase. This raises the possibility that functional impairment of GR proteins involved in synthesis of the same glycan might result in the same phenotypic abnormality. To examine this possibility, comprehensive silencing of genes encoding GR and proteoglycan core proteins was conducted in Drosophila. Drosophila GR candidate genes (125) were classified into five functional groups for synthesis of GAGs, N-linked, O-linked, Notch-related, and unknown glycans. Spatiotemporally regulated silencing caused a range of malformed phenotypes that fell into three types: extra veins, thick veins, and depigmentation. The clustered phenotypes reflected the biosynthetic pathways of GAGs, Fringe-dependent glycan on Notch, and glycans placed at or near nonreducing ends (herein termed terminal domains of glycans). Based on the phenotypic clustering, CG33145 was predicted to be involved in formation of terminal domains. Our further analysis showed that CG33145 exhibited galactosyltransferase activity in synthesis of terminal N-linked glycans. Phenotypic clustering, therefore, has potential for the functional prediction of novel GR genes.

DOI： 10.1111/gtc.12246

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Identification of β1,3-galactosyltransferases responsible for biosynthesis of insect complex-type N-glycans containing a T-antigen unit in the honeybee 査読国際誌

Tomomi Ichimiya, Megumi Maeda, Shou Sakamura, Masato Kanazawa, Shoko Nishihara, Yoshinobu Kimura

Glycoconjugate Journal 32 ( 3-4 ) 141 - 151 2015年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Kluwer Academic Publishers

Honeybees (Apis mellifera) produce unique complex-type N-glycans bearing a Galβ1-3GalNAc (T-antigen) unit, and honeybee-specific N-glycans are linked to royal jelly glycoproteins. In this study, we identified two novel honeybee β1,3-galactosyltransferase (β1,3-GalT) genes responsible for biosynthesis of the T-antigen in insect N-glycans. The products of the two putative β1,3-GalT genes (β1,3-GalT1 and β1,3-GalT2), which were expressed in Sf21 insect cells, transferred galactose (Gal) residues to GalNAc2GlcNAc2Man3GlcNAc2-PA to form the Galβ1-3GalNAc unit, indicating that the identified genes were involved in biosynthesis of the β1-3 Gal-containing N-glycan. Therefore, using biochemistry and molecular biology techniques, we revealed a unique N-glycan biosynthesis mechanism in the cephalic region of honeybees, which has not previously been found in other animal or plant cells.

DOI： 10.1007/s10719-015-9585-7

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抗腫瘍免疫への石綿曝露影響査読

武井直子, 西村泰光, 前田恵, 林宏明, 松崎秀紀, 李順姫, 吉留敬, 岸本卓巳, 福岡和也, 中野孝司, 大槻剛巳

日本予防医学会雑誌 10 ( 1 ) 1 - 8 2015年4月

Enhancement of NK Cell Cytotoxicity Induced by Long-Term Living in Negatively Charged-Particle Dominant Indoor Air-Conditions. 査読国際誌

Nishimura Y, Takahashi K, Mase A, Kotani M, Ami K, Maeda M, Shirahama T, Lee S, Matsuzaki H, Kumagai-Takei N, Yoshitome K, Otsuki T

PloS one 10 ( 7 ) e0132373 2015年

Functional Alteration of Natural Killer Cells and Cytotoxic T Lymphocytes upon Asbestos Exposure and in Malignant Mesothelioma Patients. 査読国際誌

Nishimura Y, Kumagai-Takei N, Matsuzaki H, Lee S, Maeda M, Kishimoto T, Fukuoka K, Nakano T, Otsuki T

BioMed research international 2015 238431 - 238431 2015年

Cloning and expression analysis of acidic peptide:N-glycanase genes in Solanum lycoperisicum. 査読

Fujikawa, M, Maeda, M, Fujiyama , K, Kimura, Y

Glycoconjugate Journal 32 ( 5 ) 193 2015年

Purification and characterization of Ginkgo biloba α-1,3/4-fucosidase active against Lewis a antigen 査読

Itano, S, Maeda, M, Kimura, Y

Glycoconjugate Journal 32 ( 5 ) 195 2015年

Chronic exposure to asbestos enhances TGF-β1 production in the human adult T cell leukemia virus-immortalized T cell line MT-2. 査読国際誌

Maeda M, Chen Y, Hayashi H, Kumagai-Takei N, Matsuzaki H, Lee S, Nishimura Y, Otsuki T

International journal of oncology 45 ( 6 ) 2522 - 32 2014年12月

【レギュラトリーT細胞の機能発現】制御性T細胞機能へのアスベスト曝露影響査読

西村泰光, 三浦由恵, 前田恵, 松崎秀紀, 李順姫, 武井直子, 大槻剛巳

剛巳【レギュラトリーT細胞の機能発現】制御性T細胞機能へのアスベスト曝露影響 (解説/特集) 臨床免疫・アレルギー科 62 ( 4 ) 366 - 370 2014年10月

Silica exposure and altered regulation of autoimmunity. 査読国際誌

Lee S, Matsuzaki H, Kumagai-Takei N, Yoshitome K, Maeda M, Chen Y, Kusaka M, Urakami K, Hayashi H, Fujimoto W, Nishimura Y, Otsuki T

Environmental health and preventive medicine 19 ( 5 ) 322 - 9 2014年9月

アスベスト曝露によるTh1細胞機能の低下査読

西村泰光, 前田恵, 三浦由恵, 松崎秀樹, 李順姫, 武井直子, 大槻剛巳

.臨床免疫・アレルギー科 62 ( 3 ) 314 - 317 2014年9月

珪酸曝露による免疫影響査読

松﨑秀紀, 前田恵, 林宏明, 武井直子, 李順姫, 吉留敬, 草加勝康, 浦上更三, 西村泰光, 兵藤文則, 大槻剛巳

日本予防医学会雑誌 9 ( 1 ) 1 - 8 2014年4月

Large-scale preparation of glycopeptides harboring the TF-antigen unit from royal jelly 査読国際誌

Megumi Maeda, Tatsuya Tanaka, Mariko Kimura, Yoshinobu Kimura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 78 ( 2 ) 276 - 278 2014年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

We have reported that new N-glycans carrying the TF-antigen occurred on a major royal jelly glycoprotein, and we have identified the glycosylation site to which the antigenic N-glycan is linked, but an appropriate procedure has not been established to prepare non-labeled immunoreactive glycopeptides in large amounts for functional analysis. In this study, we developed an effective method of preparing Asn-glycopeptide bearing TF-antigen.

DOI： 10.1080/09168451.2014.878223

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Functional properties of CD8(+) lymphocytes in patients with pleural plaque and malignant mesothelioma. 査読国際誌

Kumagai-Takei N, Nishimura Y, au, Maeda M, Hayashi H, Matsuzaki H, Lee S, Kishimoto T, Fukuoka K, Nakano T, Otsuki T

Journal of immunology research 2014 670140 - 670140 2014年

珪肺症例における自己寛容破綻の異常

大槻剛巳, 前田恵, 松崎秀紀, 李順姫, 武井直子, 西村泰光

日本毒性学会学術年会 41.1 O-39 2014年

記述言語：日本語出版者・発行元：日本毒性学会

珪酸とアスベストの慢性職業性曝露は塵肺症として知られる肺の繊維化を惹起する。珪酸曝露症例は珪肺症と呼ばれるが，合併症として強皮症，関節リウマチ（Caplan症候群として知られる），ANCA関連血管炎などの自己免疫疾患が知られている。従来，珪酸のアジュバントとしての作用と捉えられてきたが，我々は，珪酸曝露が直接免疫系に対して，なんらかの影響（毒性あるいは活性化）を惹起する可能性を考えて，実験系あるいは症例の末梢血単核球や，リンパ球などを用いて検討を加えてきた。アスベストについては，悪性中皮腫や肺癌などの悪性腫瘍の合併が知られており，この観点でもアスベストによる免疫系への毒性としての，腫瘍免疫の減衰について，いくつかの知見を得ているが，本演題では珪酸の免疫担当細胞への影響について，報告する。まずリンパ球の細胞死に関連が深いCD95/Fas分子について，可溶性Fasやその他のFas媒介アポトーシス阻害因子が珪肺症例で上昇していること。一方，機能性抗Fas自己抗体の検出や内因性生理的Fas誘導アポトーシスの阻害因子の珪肺症例末梢血単核球での発現減弱などの所見より，珪肺症例では慢性活性化を受け，長期生存にいたり，おそらくは自己抗原とも反応している一群と，細胞死に陥ってはリクルートされている一群がある可能性が想起された。前者は，反応性T細胞で，その他可溶性IL-2受容体の血清中での上昇や，Decoy Receptor 3 (DcR3)分子の血漿中濃度上昇，PD-1 mRNAの発現上昇などの活性化指標が検出された。一方，後者は制御性T細胞の可能性があり，珪肺症例制御性T細胞では活性化に伴うFas分子の過剰発現とともに，早期の細胞死が誘導されることが観察された。これらの免疫バランスの異常が，珪肺症例での自己寛容の破綻を惹起している可能性がある。

DOI： 10.14869/toxpt.41.1.0_o-39

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Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos. 査読国際誌

Maeda M, Chen Y, Kumagai-Takei N, Hayashi H, Matsuzaki H, Lee S, Hiratsuka J, Nishimura Y, Kimura Y, Otsuki T

Immunobiology 218 ( 9 ) 1184 - 91 2013年9月

Purification and Substrate Specificity of A Ginkgo biloba Glycosidase Active in beta-1,2-Xylosidic Linkage in Plant Complex Type N-Glycans 査読国際誌

Megumi Maeda, Tsuyoshi Akiyama, Daisuke Yokouchi, Kwan Kit Woo, Yoshinobu Kimura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 9 ) 1973 - 1976 2013年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

The beta-xylosidase, which is active against plant complex type N-glycans, was purified to homogeneity from Ginkgo biloba seeds. The N-terminal amino acid sequence, G-S-A-A-G-N-R-, of the Ginkgo beta-xylosidase (beta-Xyl'ase Gb) was consistent with the deduced internal amino acid sequence of an Arabidopsis beta-xylosidase (AtBXL1). beta-Xyl'ase Gb hydrolyzed the beta 1-2 xylosyl residue from Xyl beta 1-2Man beta 1-4G1cNAc beta 1-4GlcNAc-PA and Xyl beta 1-2Man beta 1-4G1cNA beta 1-4(Fuc alpha 1-3)GlcNAc-PA, but not that from Manal-6(Man alpha 1-3)(Xy1 beta 1-2)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-3)GlcNAc-PA.

DOI： 10.1271/bbb.130303

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論文 - 前田 恵

<前田　恵>

論文 - 前田　恵