Updated on 2024/12/26

写真a

 
FUJITA Hirofumi
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Assistant Professor
Position
Assistant Professor
External link

Degree

  • 博士(医学) ( 岡山大学 )

Research Interests

  • 分化

  • がん

  • Free radical

  • Cell death

  • Cancer

  • Bone metabolism

  • Cell differentiation

  • 骨代謝

  • 活性酸素

  • 細胞死

Research Areas

  • Life Science / Anatomy

  • Life Science / Medical biochemistry

  • Life Science / Cell biology

  • Life Science / Orthopedics

Education

  • Okayama University    

    - 2005

      More details

  • Okayama University   医学系研究科  

    - 2005

      More details

    Country: Japan

    researchmap

Research History

  • - Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2006

      More details

  • - 岡山大学医歯薬学総合研究科 助教

    2006

      More details

Professional Memberships

Committee Memberships

  • 日本酸化ストレス学会   評議員  

    2008   

      More details

    Committee type:Academic society

    日本酸化ストレス学会

    researchmap

 

Papers

  • Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse. International journal

    Shiori Ikeda, Keita Sato, Hirofumi Fujita, Hitomi Ono-Minagi, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

    Journal of developmental biology   12 ( 2 )   2024.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The mouse Harderian gland (HG) is a secretory gland that covers the posterior portion of the eyeball, opening at the base of the nictitating membrane. The HG serves to protect the eye surface from infection with its secretions. Mice open their eyelids at about 2 weeks of age, and the development of the HG primordium mechanically opens the eye by pushing the eyeball from its rear. Therefore, when HG formation is disturbed, the eye exhibits enophthalmos (the slit-eye phenotype), and a line of Fgf10+/- heterozygous loss-of-function mice exhibits slit-eye due to the HG atrophy. However, it has not been clarified how and when HGs degenerate and atrophy in Fgf10+/- mice. In this study, we observed the HGs in embryonic (E13.5 to E19), postnatal (P0.5 to P18) and 74-week-old Fgf10+/- mice. We found that more than half of the Fgf10+/- mice had markedly degenerated HGs, often unilaterally. The degenerated HG tissue had a melanized appearance and was replaced by connective tissue, which was observed by P10. The development of HGs was delayed or disrupted in the similar proportion of Fgf10+/- embryos, as revealed via histology and the loss of HG-marker expression. In situ hybridization showed Fgf10 expression was observed in the Harderian mesenchyme in wild-type as well as in the HG-lacking heterozygote at E19. These results show that the Fgf10 haploinsufficiency causes delayed or defective HG development, often unilaterally from the unexpectedly early neonatal period.

    DOI: 10.3390/jdb12020016

    PubMed

    researchmap

  • システイニルロイコトリエン受容体CysLTR1は破骨細胞分化と骨吸収に必須ではない

    藤田 洋史, 服部 高子, 久保田 聡

    日本骨代謝学会学術集会プログラム抄録集   41回   162 - 162   2023.7

     More details

    Language:Japanese   Publisher:(一社)日本骨代謝学会  

    researchmap

  • Protrusion of KCNJ13 Gene Knockout Retinal Pigment Epithelium Due to Oxidative Stress–Induced Cell Death

    Yuki Kanzaki, Hirofumi Fujita, Keita Sato, Mio Hosokawa, Hiroshi Matsumae, Yuki Morizane, Hideyo Ohuchi

    Investigative Opthalmology & Visual Science   63 ( 12 )   29 - 29   2022.11

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Association for Research in Vision and Ophthalmology (ARVO)  

    DOI: 10.1167/iovs.63.12.29

    researchmap

  • Cysteinyl leukotriene receptor 1 is dispensable for osteoclast differentiation and bone resorption

    Hirofumi Fujita, Aoi Ando, Yohei Mizusawa, Mitsuaki Ono, Takako Hattori, Munenori Habuta, Toshitaka Oohashi, Satoshi Kubota, Hideyo Ohuchi

    PLOS ONE   17 ( 11 )   e0277307 - e0277307   2022.11

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Public Library of Science (PLoS)  

    Cysteinyl leukotriene receptor 1 (CysLTR1) is a G protein-coupled receptor for the inflammatory lipid mediators cysteinyl leukotrienes, which are involved in smooth muscle constriction, vascular permeability, and macrophage chemokine release. The Cysltr1 gene encoding CysLTR1 is expressed in the macrophage lineage, including osteoclasts, and the CysLTR1 antagonist Montelukast has been shown to suppress the formation of osteoclasts. However, it currently remains unclear whether CysLTR1 is involved in osteoclast differentiation and bone loss. Therefore, to clarify the role of CysLTR1 in osteoclastogenesis and pathological bone loss, we herein generated CysLTR1 loss-of-function mutant mice by disrupting the cysltr1 gene using the CRISPR-Cas9 system. These mutant mice had a frameshift mutation resulting in a premature stop codon (Cysltr1 KO) or an in-frame mutation causing the deletion of the first extracellular loop (Cysltr1Δ105). Bone marrow macrophages (BMM) from these mutant mice lost the intracellular flux of calcium in response to leukotriene D4, indicating that these mutants completely lost the activity of CysLTR1 without triggering genetic compensation. However, disruption of the Cysltr1 gene did not suppress the formation of osteoclasts from BMM in vitro. We also demonstrated that the CysLTR1 antagonist Montelukast suppressed the formation of osteoclasts without functional CysLTR1. On the other hand, disruption of the Cysltr1 gene partially suppressed the formation of osteoclasts stimulated by leukotriene D4 and did not inhibit that by glutathione, functioning as a substrate in the synthesis of cysteinyl leukotrienes. Disruption of the Cysltr1 gene did not affect ovariectomy-induced osteoporosis or lipopolysaccharide-induced bone resorption. Collectively, these results suggest that the CysLT-CysLTR1 axis is dispensable for osteoclast differentiation in vitro and pathological bone loss, while the leukotriene D4-CysTR1 axis is sufficient to stimulate osteoclast formation. We concluded that the effects of glutathione and Montelukast on osteoclast formation were independent of CysLTR1.

    DOI: 10.1371/journal.pone.0277307

    researchmap

  • Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice. International journal

    Ruizhi Xue, Wenfeng Lin, Hirofumi Fujita, Jingkai Sun, Rie Kinoshita, Kazuhiko Ochiai, Junichiro Futami, Masami Watanabe, Hideyo Ohuchi, Masakiyo Sakaguchi, Zhengyan Tang, Peng Huang, Yasutomo Nasu, Hiromi Kumon

    Genes   13 ( 2 )   2022.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 ± 8.12% vs. 23.26 ± 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 ± 0.69 vs. 8.27 ± 0.87, ×106), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 ± 1.55% vs. 72.50 ± 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice.

    DOI: 10.3390/genes13020285

    PubMed

    researchmap

▼display all

Books

  • CATALASE GENE MUTANT MICE: ACATALASEMIC AND HYPOCATALASEMIC MICE.

    Advances in Medicine and Biology.  2013 

     More details

MISC

  • ゲノム編集Fgf10モザイク変異体の遺伝子型と表現型解析

    土生田宗憲, 泰江章博, 鈴木賢一, 藤田洋史, 高山鮎子, 佐藤恵太, 板東哲哉, 親泊政一, 田中栄二, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   125th   2020

  • Fgf10モザイク変異体における肺表現型および下肢帯表現型の形態学的解析

    土生田宗憲, 泰江章博, 鈴木賢一, 高山鮎子, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本先天異常学会学術集会プログラム・抄録集   60th   2020

  • KCNJ13遺伝子欠損がヒト多能性幹細胞由来網膜色素上皮細胞の貪食能に及ぼす影響

    神崎勇希, 神崎勇希, 藤田洋史, 佐藤恵太, 細川海音, 松前洋, 白神史雄, 森實祐基, 大内淑代

    日本眼科学会雑誌   124   2020

  • 神経網膜形成における転写因子Lhx1の下流遺伝子の機能解析

    衣畑俊希, 佐藤恵太, 藤田洋史, 板東哲哉, 濃野勉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

  • REIC/Dickkopf3(Dkk3)の副腎における機能解析

    土生田宗憲, 藤田洋史, 佐藤恵太, 板東哲哉, 公文裕巳, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   124th   2019

▼display all

Presentations

  • ABCG2 siRNA導入によるがん細胞のアポトーシス誘導

    2017年度生命科学系学会合同年次大会  2017 

     More details

  • CRISPR/Cas9システムにより作製したモザイク変異マウスの組織学的解析

    2017年度生命科学系学会合同年次大会  2017 

     More details

  • ノックアウトマウス及びプロテオーム解析を用いたDKK3タンパク質の生理的機能の解明

    第122回日本解剖学会総会・全国学術集会  2017 

     More details

  • 抗酸化物質グルタチオンは炎症性骨破壊を促進する

    第34回日本骨代謝学会学術集会 第3回アジア太平洋骨代謝学会議  2016 

     More details

  • ヘム代謝制御に基づく光感受性物質protoporphyrin IX蓄積機構の研究

    第69回日本酸化ストレス学会学術集会  2016 

     More details

▼display all

Awards

  • 岡山医学会賞がん研究奨励賞(林原賞・山田賞)

    2017  

     More details

    Country:Japan

    researchmap

  • 日本過酸化脂質・フリーラジカル学会(現 酸化ストレス学会)論文賞

    2007  

     More details

  • アンチオキシダントバイオファクター研究会 奨励賞

    2007  

     More details

  • 第46回日本生化学会中国四国支部例会 学術奨励賞

    2006  

     More details

 

Class subject in charge

  • Primary Anatomy (2024academic year) special  - その他

  • Human Anatomy (2024academic year) Concentration  - その他

  • Human Embryology (2024academic year) special  - その他

  • Anatomy and Histology (2024academic year) special  - その他

  • Practice in Anatomy and Histology (2024academic year) special  - その他

▼display all