Faculty Profiles - TAKIGAWA Masaharu

MISC - TAKIGAWA Masaharu

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Development of The Dentin Regeneration Therapy-Expression of Type1 collagen and ALP induced by CTGF in human cultured dental pulp-

Hirotoshi Shimizu, Yoshihiro Nishitani, Tomiko Yamada, Takashi Nishida, Masaharu Takigawa, Masahiro Yoshiyama, Dept of Operative Dentistry Okayama Univ. Graduate School of Medicine and Dentistry, Dept of Operative Dentistry Okayama Univ. Graduate School of Medicine and Dentistry, Dept of Operative Dentistry Okayama Univ. Graduate School of Medicine and Dentistry, Dept of Biochemistry Okayama Univ. Graduate School of Medicine and Dentistry, Dept of Biochemistry Okayama Univ. Graduate School of Medicine and Dentistry, Dept of Operative Dentistry Okayama Univ. Graduate School of Medicine and Dentistry

Journal of hard tissue biology = Journal of hard tissue biology 11 ( 2 ) 76 - 76 2002.12

Hepatocyte growth factor counteracts transforming growth factor-beta(1), through attenuation of connective tissue growth factor induction, and prevents renal fibrogenesis in 5/6 nephrectomized mice

T Inoue, H Okada, T Kobayashi, Y Watanabe, Y Kanno, JB Kopp, T Nishida, M Takigawa, M Ueno, T Nakamura, H Suzuki

FASEB JOURNAL 16 ( 14 ) 268 - + 2002.12

Language：English Publisher：FEDERATION AMER SOC EXP BIOL

We investigated the mechanism of the anti-fibrotic effects of hepatocyte growth factor (HGF) in the kidney, with respect to its effect on connective tissue growth factor (CTGF), a down-stream, profibrotic mediator of transforming growth factor-beta(1) (TGF-beta(1)). In wild-type (WT) mice with 5/6 nephrectomy (Nx), HGF and TGF-beta(1) mRNAs increased transiently in the remnant kidney by week 1 after the Nx, returned to baseline levels, and increased again at weeks 4 to 12. In contrast, CTGF and 1(I) procollagen (COLI) mRNAs increased in parallel with HGF and TGF-beta(1) during the early stage, but did not re-increase during the late stage. In the case of TGF-beta(1) transgenic (TG) mice with 5/6 Nx, excess TGF-beta(1) derived from the transgene enhanced CTGF expression significantly in the remnant kidney, accordingly accelerating renal fibrogenesis. Administration of dHGF (5.0 mg/kg/day) to TG mice with 5/6 Nx for 4 weeks from weeks 2 to 6 suppressed CTGF expression in the remnant kidney, attenuating renal fibrosis and improving the survival rate. In an experiment in vitro, renal tubulointerstitial fibroblasts (TFB) were co-cultured with proximal tubular epithelial cells (PTEC). Pretreatment with HGF reduced significantly CTGF induction in PTEC by TGF-beta(1), consequently suppressing COLI synthesis in TFB. In conclusion, HGF can block, at least partially, renal fibrogenesis promoted by TGF-beta1 in the remnant kidney, via attenuation of CTGF induction.

DOI： 10.1096/fj.02-0442fje

Web of Science

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ヒト口腔扁平上皮癌細胞株における結合組織成長因子(CTGF)の腫瘍細胞増殖抑制効果

森谷徳文, 久保田聡, 近藤誠二, 西田崇, 川木晴美, 菅原利夫, 滝川正春

歯科基礎医学会雑誌 44 ( 5 ) 395 - 395 2002.9

肥大軟骨細胞特異的遺伝子産物CTGF/Hcs24のモジュール特異的抗体の解析とその軟骨細胞分化促進効果

湊雅直, 久保田聡, 川木晴美, 西田崇, 中西徹, 山本照子, 滝川正春

歯科基礎医学会雑誌 44 ( 5 ) 388 - 388 2002.9

硬組織発生過程でのerbB4遺伝子発現

山合友一朗, 中西徹, 縄稚久美子, 井上美穂, 杉本朋貞, 滝川正春

歯科基礎医学会雑誌 44 ( 5 ) 435 - 435 2002.9

結合組織成長因子CTGF/Hcs24遺伝子の軟骨由来細胞におけるグルココルチコイドによる発現誘導

久保田聡, 森谷徳文, 三村晴世, 川木晴美, 湊雅直, 滝川正春

歯科基礎医学会雑誌 44 ( 5 ) 437 - 437 2002.9

チロシンキナーゼ型レセプターErbB4遺伝子の軟骨細胞における発現

縄稚久美子, 久保田聡, 西田崇, 吉道玄, 中西徹, 完山学, 窪木拓男, 矢谷博文, 山合友一郎, 滝川正春

歯科基礎医学会雑誌 44 ( 5 ) 388 - 388 2002.9

DNAマイクロアレイによる間葉系幹細胞の発現プロファイリング

中西徹, 大山和美, 滝川正春

歯科基礎医学会雑誌 44 ( 5 ) 389 - 389 2002.9

Novel cis-element TRENDIC that enhance connective tissue growth factor (ctgf) gene expression in chondrocytic HCS-2/8.

T Eguchi, S Kubota, S Kondo, Y Mukudai, T Kuboki, H Yatani, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S224 - S224 2002.9

Analysis of gene expression in osteoblastic cell stimulated by connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24).

E Nakata, T Nakanishi, T Nishida, A Kawai, H Doi, H Inoue, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S224 - S224 2002.9

Effects of IL-1beta and LPS on CTGF expression in mouse-derived odontoblast-like cells, MDPC-23.

W Sonoyama, T Kuboki, T Fujisawa, T Eguchi, J Uehara, H Yatani, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S327 - S327 2002.9

CTGF/Hcs24, a hypertrophic chondrocyte-specific gene product, stimulates proliferation and differentiation but not hypertrophy of cultured articular chondrocytes.

T Nishida, S Kubota, T Nakanishi, T Kuboki, G Yosimichi, S Kondo, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S180 - S181 2002.9

Role of connective tissue growth factor and its intracellular signaling in podocytes.

M Koshikawa, K Mori, M Mukoyama, A Sugawara, T Suganami, K Sawai, H Yokoi, N Kobayashi, M Takigawa, P Mundel, K Nakao

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 13 314A - 314A 2002.9

Hepatocyte growth factor counteracts transforming growth factor-betal via attenuation of connective tissue growth factor induction and prevents renal fibrogenesis in 5/6 nephrectomized mice.

T Inoue, H Okada, Y Kanno, T Kobayashi, Y Watanabe, K Kikuta, JB Kopp, M Takigawa, T Nakamura, H Suzuki

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 13 746A - 747A 2002.9

CTGF/Hcs24 induces chondrocyte differentiation through p38 mitogen-activated protein kinase (p38MAPK), and proliferation through p44/42 MAPK/extracellular-signal regulated kinase (ERK).

G Yosimichi, T Nakanishi, T Nishida, T Hattori, T Takano-Yamamoto, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S222 - S223 2002.9

Kunitz-type protease inhibitor bikunin disrupts phorbol ester-induced oligomerization of CD44 variant isoforms containing epitope v9 and subsequently suppresses expression of urokinase-type plasminogen activator in human chondrosarcoma cells. (vol 277, pg 8022, 2002)

M Suzuki, H Kobayashi, M Fujie, T Nishida, M Takigawa, N Kanayama, T Terao

JOURNAL OF BIOLOGICAL CHEMISTRY 277 ( 18 ) 16346 - 16346 2002.5

REGULATION OF BIGLYCAN AND DECORIN SYNTHESIS BY CONNECTIVE TISSUE GROWTH FACTOR IN CULTURED BOVINE AORTIC ENDOTHELIAL CELLS

Yamamoto Chika, Oh-i Mami, Fujiwara Yasuyuki, Kaji Toshiyuki, Nishida Takashi, Nakanishi Tohru, Takigawa Masaharu, Kinsella Michael G., Wight Thomas N.

Connective tissue 34 ( 1 ) 50 - 50 2002.4

Language：English Publisher：The Japanese Society for Connective Tissue

Connective tissue growth factor (CTGF) is a regulator of vascular endothelial cell functions but little is know about the regulation of proteoglycan synthesis by the growth factor. Since endothelial cell proteoglycan synthesis is often regulated depending on the cell density, dense and sparse cultures of bovine aortic endothelial cells were metabolically labeled with [^<35>S]sulfate or ^<35>S-labaled amino acids in the presence of recombinant human CTGF. The labeled proteoglycans were characterized by DEAE-Sephacel ion exchange chromatography and Sepharose CL-4B molecular sieve chromatography. The glycosaminoglycan (GAGs) M_r and composition were analyzed by Sepharose CL-6B chromatography, and the core protein M_r was analyzed by SDS-polyacrylamide gel electrophoresis, before and after digestion with papain, heparitinase or chondroitin ABC lyase. The core proteins were identified by Western blot analysis and core protein mRNAs were determined by quantitative RT-PCR. The results indicated that CTGF suppresses the synthesis of biglycan but newly induces that of decorin in endothelial cells when the cell density is low. However, neither the hydrodynamic size nor the GAG chain length of these two small chondroitin/dermatan sulfate proteoglycans was changed by the growth factor. The present data suggest that CTGF is a regulator of the synthesis of small chondroitin/dermatan sulfate proteoglycans, biglycan in vascular endothelial cells depending on the cell density.

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Effects of proinflammatory factors on CTGF expression in odontoblast-like cells

W Sonoyama, T Kuboki, T Fujisawa, T Eguchi, J Uehara, S Takashiba, H Yatani, M Takigawa

JOURNAL OF DENTAL RESEARCH 81 A155 - A155 2002.3

Expression of CTGF in endochondral and intramembranous ossification during mandibular fracture healing

T Fukunaga, T Yamashiro, K Yamashita, JN Pereira, M Takigawa, T Takano-Yamamoto

JOURNAL OF DENTAL RESEARCH 81 A190 - A190 2002.3

CTGF upregulation observed in the rat tooth extraction sockets.

M Kanyama, T Kuboki, K Akiyama, F Miyauchi, K Nawachi, H Yatani, S Kubota, T Nakanishi, M Takigawa

JOURNAL OF DENTAL RESEARCH 81 A107 - A107 2002.3

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