2023/01/27 更新

写真a

ヤマモト ユミコ
山本 由弥子
YAMAMOTO Yumiko
所属
医歯薬学域 助教
職名
助教
外部リンク

学位

  • 博士(理学) ( 岡山大学 )

  • 修士(理学) ( 岡山大学 )

研究キーワード

  • Clostridium botulinum

  • Neurotoxin

  • 神経毒素

  • ボツリヌス菌

研究分野

  • ライフサイエンス / 細菌学

  • ライフサイエンス / 分子生物学

  • ライフサイエンス / 機能生物化学

 

論文

  • Peripherally Administered Botulinum Toxin Type A Localizes Bilaterally in Trigeminal Ganglia of Animal Model 査読

    Arief Waskitho, Yumiko Yamamoto, Swarnalakshmi Raman, Fumiya Kano, Huijiao Yan, Resmi Raju, Shaista Afroz, Tsuyoshi Morita, Daisuke Ikutame, Kazuo Okura, Masamitsu Oshima, Akihito Yamamoto, Otto Baba, Yoshizo Matsuka

    Toxins   13 ( 10 )   704 - 704   2021年10月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Peripheral nerve injury leads to sensory ganglion hyperexcitation, which increases neurotransmitter release and neuropathic pain. Botulinum toxin type A (BoNT/A) regulates pain transmission by reducing neurotransmitter release, thereby attenuating neuropathic pain. Despite multiple studies on the use of BoNT/A for managing neuropathic pain in the orofacial region, its exact mechanism of transport remains unclear. In this study, we investigated the effects of BoNT/A in managing neuropathic pain in two different animal models and its transport mechanism in the trigeminal nerve. Intraperitoneal administration of cisplatin induced bilateral neuropathic pain in the orofacial region, reducing the head withdrawal threshold to mechanical stimulation. Unilateral infraorbital nerve constriction (IONC) also reduced the ipsilateral head withdrawal threshold to mechanical stimulation. Unilateral peripheral administration of BoNT/A to the rat whisker pad attenuated cisplatin-induced pain behavior bilaterally. Furthermore, contralateral peripheral administration of BoNT/A attenuated neuropathy-induced behavior caused by IONC. We also noted the presence of BoNT/A in the blood using the mouse bioassay. In addition, the Alexa Fluor-488-labeled C-terminal half of the heavy chain of BoNT/A (BoNT/A-Hc) was localized in the neurons of the bilateral trigeminal ganglia following its unilateral administration. These findings suggest that axonal and hematogenous transport are involved in the therapeutic effects of peripherally administered BoNT/A in the orofacial region.

    DOI: 10.3390/toxins13100704

    researchmap

  • Elizabethkingia anophelis, an emerging pathogen, inhibits RAW 264.7 macrophage function 査読

    I Putu Bayu Mayura, Kazuyoshi Gotoh, Hayato Nishimura, Erina Nakai, Takehiko Mima, Yumiko Yamamoto, Kenji Yokota, Osamu Matsushita

    Microbiology and Immunology   65 ( 8 )   317 - 324   2021年8月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Elizabethkingia anophelis is a pathogen that can cause a life-threatening infection in immunocompromised patients. The first case of E. anophelis infection was reported in 2013; subsequently, an increase in its incidence has been reported globally. Additionally, a mortality rate of more than 30% was observed in the US outbreak of 2015. To date, the pathogenic mechanisms underlying E. anophelis infection, such as toxin production, remain unclear. Since tissue macrophages act as the first line of defense against pathogens, in the present study the interactions between E. anophelis and a macrophage-like cell line RAW 264.7 were examined. Although E. anophelis showed no cytotoxicity toward RAW 264.7 macrophages, the infection inhibited LPS-induced morphological changes and activation of differentiation markers for the polarization of RAW 264.7 macrophages toward an M1-like phenotype. However, when the cell contact was restricted using Transwell inserts or bacterial culture supernatants were used instead of live bacteria, no such inhibition was observed. Moreover, it was shown that E. anophelis evaded phagocytosis. Overall, the results suggest that E. anophelis infection inhibits the differentiation of RAW 264.7 macrophages to a pro-inflammatory phenotype in a contact-dependent manner.

    DOI: 10.1111/1348-0421.12888

    Scopus

    PubMed

    researchmap

    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1348-0421.12888

  • Environmental survey of Methicillin-Resistant Staphylococci in a Hospital in Japan 査読

    AKARI WATANABE, TOKIKO WATANABE, SUSUMU KOKEGUCHI, YUMIKO YAMAMOTO, OSAMU MATSUSHITA, KENJI YOKOTA

    Biocontrol Science   26 ( 3 )   137 - 145   2021年

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:The Society for Antibacterial and Antifungal Agents, Japan  

    DOI: 10.4265/bio.26.137

    researchmap

  • Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense 査読

    Yoshihiko Sakaguchi, Jumpei Uchiyama, Akira Také, Kazuyoshi Gotoh, Masakiyo Sakaguchi, Tomonori Suzuki, Yumiko Yamamoto, Koji Hosomi, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki, Shunji Hayashi, Keiji Oguma

    Anaerobe   66   102281 - 102281   2020年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.anaerobe.2020.102281

    researchmap

  • Vibrio alginolyticus VepA Induces Lysosomal Membrane Permeability and Cathepsin-Independent Cell Death. 査読

    Agus Eka Darwinata, Kazuyoshi Gotoh, Takehiko Mima, Yumiko Yamamoto, Kenji Yokota, Osamu Matsushita

    Acta Medica Okayama   72 ( 3 )   231 - 239   2018年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The bacterium Vibrio alginolyticus, an opportunistic pathogen in humans, has a type III secretion system (T3SS) that is responsible for its cytotoxicity toward eukaryotic cells. The effector of T3SS that is responsible for the cytotoxicity had not been identified. Here we demonstrate that VepA, a homolog of the T3SS effector in V. parahaemolyticus, is required for cytotoxicity in V. alginolyticus. VepA induces lysosomal membrane permeabilization, and it allows the leakage of only small molecules into the cytosol. Our findings revealed that VepA induces cathepsin-independent cell death in mammalian cells. The ferrous ion, one of the small molecules in the lysosome contents, appears to be involved in the cell death caused by V. alginolyticus VepA.

    DOI: 10.18926/AMO/56068

    PubMed

    researchmap

  • Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus 査読

    Takehiko Mima, Kazuyoshi Gotoh, Yumiko Yamamoto, Keiko Maeda, Taku Shirakawa, Shunsuke Matsui, Yumi Murata, Takaki Koide, Hiroshi Tokumitsu, Osamu Matsushita

    The Journal of Membrane Biology   251 ( 1 )   51 - 63   2018年2月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00232-017-9991-9

    researchmap

    その他リンク: http://link.springer.com/content/pdf/10.1007/s00232-017-9991-9.pdf

  • Botulinum neurotoxin type A alleviates mechanical hypersensitivity associated with infraorbital nerve constriction injury in rats 査読

    Noboru Noma, Kosuke Watanabe, Yuka Sato, Yoshiki Imamura, Yumiko Yamamoto, Reio Ito, Mitsuru Maruno, Kohei Shimizu, Koichi Iwata

    Neuroscience Letters   637   96 - 101   2017年1月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.neulet.2016.11.043

    researchmap

  • Cross-Excitation in Peripheral Sensory Ganglia Associated with Pain Transmission 査読

    Katsuhiro Omoto, Kotaro Maruhama, Ryuji Terayama, Yumiko Yamamoto, Osamu Matsushita, Tomosada Sugimoto, Keiji Oguma, Yoshizo Matsuka

    Toxins   7 ( 8 )   2906 - 2917   2015年8月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    DOI: 10.3390/toxins7082906

    researchmap

  • Genomics of Clostridium botulinum group III strains 査読

    Yoshihiko Sakaguchi, Tomonori Suzuki, Yumiko Yamamoto, Atsushi Nishikawa, Keiji Oguma

    Research in Microbiology   166 ( 4 )   318 - 325   2015年5月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.resmic.2014.07.016

    researchmap

  • Phospholipase C Produced by Clostridium botulinum Types C and D: Comparison of Gene, Enzymatic, and Biological Activities with Those of Clostridium perfringens Alpha-toxin 査読

    Ni Nengah Dwi Fatmawati, Yoshihiko Sakaguchi, Tomonori Suzuki, Masataka Oda, Kenta Shimizu, Yumiko Yamamoto, Jun Sakurai, Osamu Matsushita, Keiji Oguma

    Acta Medica Okayama   67 ( 1 )   9 - 18   2013年2月

     詳細を見る

    記述言語:英語   出版者・発行元:Okayama University Medical School  

    Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival.

    DOI: 10.18926/AMO/49252

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://search.jamas.or.jp/link/ui/2013249132

  • Intraprostatic Botulinum Neurotoxin Type A Injection for Benign Prostatic Hyperplasia: Preliminary Results with a Newly Purified Neurotoxin 査読

    Teruhiko Yokoyama, Yumiko Yamamoto, Tomonori Suzuki, Keiji Oguma, Atsushi Nagai

    Acta Medica Okayama   66 ( 4 )   291 - 297   2012年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Okayama University Medical School  

    Several studies have demonstrated the efficacy of intraprostatic injection of botulinum neurotoxin type A (BoNT/A) against symptomatic benign prostatic hyperplasia (BPH). The most commonly used BoNT/A product, Botox®, forms large complexes and composed of neurotoxin (NTX) as well as non-toxic components. We purified NTX lacking non-toxic components. We investigated the efficacy of this newly purified NTX for men with BPH. Ten male patients (mean age, 70.0 years) with BPH received 100 units (prostate volume [PV] <30ml) or 200 units (PV ァ30ml) of NTX injected into the prostate via a minimally invasive outpatient technique. Evaluation included uroflowmetry, postvoid residual urine volume (PVR), PV, and International Prostate Symptom Score (IPSS) measured at baseline and 1, 3, 6, and 12 months post-treatment. The status of 7 of the 10 patients examined was found to have improved within 1 month of treatment. The mean IPSS decreased from 23.8±7.0 to 16.3±10.3 (p=0.0093) at 1 month, to 14.9±8.2 (p=0.0074) at 3 months, and to 16.9±7.3 (p=0.018) at 12 months. The mean PV decreased from 47.8±21.2 to 39.2±19.5ml (p=0.0076) at 3 months. The PVR improved at 3 and 6 months post-treatment. Intraprostatic NTX injection induces prostate shrinkage and is effective in men with BPH.

    DOI: 10.18926/AMO/48668

    Scopus

    PubMed

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://search.jamas.or.jp/link/ui/2013306579

  • Clostridium botulinum Type E Toxins Bind to Caco-2 Cells by a Different Mechanism from That of Type A Toxins 査読

    Zhang Kai, Yamamoto Yumiko, Suzuki Tomonori, Yokota Kenji, Ma Shaobo, Nengah Dwi Fatmawati Ni, Oguma Keiji

    Acta Medica Okayama   66 ( 3 )   253 - 261   2012年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Okayama University Medical School  

    Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (Hc). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or Hc. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E Hc significantly inhibited the 7S toxin binding, indicating that Hc might be a main binding domain of the type E toxin.

    DOI: 10.18926/AMO/48565

    Scopus

    PubMed

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://search.jamas.or.jp/link/ui/2013156720

  • Intradermal injection of Botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay 査読

    A. KUMADA, Y. MATSUKA, I. SPIGELMAN, K. MARUHAMA, Y. YAMAMOTO, J. K. NEUBERT, T. A. NOLAN, K. WATANABE, K. MAEKAWA, H. KAMIOKA, T. YAMASHIRO, T. KUBOKI, K. OGUMA

    Journal of Oral Rehabilitation   39 ( 1 )   63 - 72   2012年1月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1365-2842.2011.02236.x

    researchmap

  • Application of Purified Botulinum Type A Neurotoxin to Treat Experimental Trigeminal Neuropathy in Rats and Patients with Urinary Incontinence and Prostatic Hyperplasia 査読

    Yoshizo Matsuka, Teruhiko Yokoyama, Yumiko Yamamoto, Tomonori Suzuki, Ni Nengah Dwi Fatmawati, Atsushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Takuo Kuboki, Atsushi Nagai, Keiji Oguma

    Journal of Toxicology   2012   1 - 8   2012年

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Hindawi Limited  

    Type A neurotoxin (NTX) of<italic>Clostridium botulinum</italic>was purified by a simple procedure using a lactose gel column. The toxicity of this purified toxin preparation was retained for at least 1 year at −30°C by supplementation with either 0.1% albumin or 0.05% albumin plus 1% trehalose. When purified NTX was used to treat 49 patients with urinary incontinence caused by either refractory idiopathic or neurogenic detrusor overactivity, 36 patients showed significant improvement in symptoms. These beneficial effects were also observed in cases of prostatic hyperplasia. The results obtained with NTX were similar to that of Botox. The effects of NTX on trigeminal neuralgia induced by infraorbital nerve constriction (IoNC) in rats were also studied. Trigeminal ganglion neurons from ipsilateral to IoNC exhibited significantly faster onset of FM4-64 release than sham-operated contralateral neurons. Intradermal injection of NTX in the area of IoNC alleviated IoNC-induced pain behavior and reduced the exaggerated FM4-64 release in trigeminal ganglion neurons.

    DOI: 10.1155/2012/648384

    researchmap

    その他リンク: http://downloads.hindawi.com/journals/jt/2012/648384.xml

  • コレラ菌およびコレラ毒素Bサブユニットに対するニワトリ抗体(IgY)の有用性

    平井 一行, 衛藤 友美, 大野 佑子, 田村 臣哉, 山本 由弥子, 難波 ひかる, 阪口 義彦, 横田 憲治, 小熊 惠二

    日本薬学会年会要旨集   130年会 ( 3 )   84 - 84   2010年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本薬学会  

    researchmap

  • Effects of Purified Newly Developed Botulinum Neurotoxin Type A in Rat Prostate 査読

    Yasuhiro Nishiyama, Teruhiko Yokoyama, Kazuhito Tomizawa, Kikuo Okamura, Yumiko Yamamoto, Hideki Matsui, Keiji Oguma, Atsushi Nagai, Hiromi Kumon

    Urology   74 ( 2 )   436 - 439   2009年8月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.urology.2009.01.047

    researchmap

  • Knockdown of the PsbP protein does not prevent assembly of the dimeric PSII core complex but impairs accumulation of photosystem II supercomplexes in tobacco 査読

    Kunio Ido, Kentaro Ifuku, Yumiko Yamamoto, Seiko Ishihara, Akio Murakami, Keiji Takabe, Chikahiro Miyake, Fumihiko Sato

    Biochimica et Biophysica Acta (BBA) - Bioenergetics   1787 ( 7 )   873 - 881   2009年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.bbabio.2009.03.004

    researchmap

  • Molecular Analysis of an Extrachromosomal Element Containing the C2 Toxin Gene Discovered in Clostridium botulinum Type C 査読

    Yoshihiko Sakaguchi, Tetsuya Hayashi, Yumiko Yamamoto, Keisuke Nakayama, Kai Zhang, Shaobo Ma, Hideyuki Arimitsu, Keiji Oguma

    Journal of Bacteriology   191 ( 10 )   3282 - 3291   2009年5月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>

    <italic>Clostridium botulinum</italic>
    cultures are classified into seven types, types A to G, based on the antigenicity of the neurotoxins produced. Of these seven types, only types C and D produce C2 toxin in addition to the neurotoxin. The C2 toxin consists of two components designated C2I and C2II. The genes encoding the C2 toxin components have been cloned, and it has been stated that they might be on the cell chromosome. The present study confirmed by using pulsed-field gel electrophoresis and subsequent Southern hybridization that these genes are on a large plasmid. The complete nucleotide sequence of this plasmid was determined by using a combination of inverse PCR and primer walking. The sequence was 106,981 bp long and contained 123 potential open reading frames, including the
    <italic>c2I</italic>
    and
    <italic>c2II</italic>
    genes. The 57 products of these open reading frames had sequences similar to those of well-known proteins. It was speculated that 9 these 57 gene products were related to DNA replication, 2 were responsible for the two-component regulatory system, and 3 were σ factors. In addition, a total of 20 genes encoding proteins related to diverse processes in purine catabolism were found in two regions. In these regions, there were 9 and 11 genes rarely found in plasmids, indicating that this plasmid plays an important role in purine catabolism, as well as in C2 toxin production.

    DOI: 10.1128/jb.01797-08

    researchmap

  • Botulinum toxin type a (150 kDa) decreases exaggerated neurotransmitter release from trigeminal ganglion neurons and relieves neuropathy behaviors induced by infraorbital nerve constriction 査読

    Y. Kitamura, Y. Matsuka, I. Spigelman, Y. Ishihara, Y. Yamamoto, W. Sonoyama, T. Kuboki, K. Oguma

    Neuroscience   159 ( 4 )   1422 - 1429   2009年4月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.neuroscience.2009.01.066

    researchmap

  • Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection 査読

    Kiyoshi Ayada, Kenji Yokota, Yoshiro Kawahara, Yumiko Yamamoto, Kazuyuki Hirai, Tomoki Inaba, Masahide Kita, Hiroyuki Okada, Kazuhide Yamamoto, Keiji Oguma

    CLINICAL & DEVELOPMENTAL IMMUNOLOGY   2009年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:HINDAWI PUBLISHING CORPORATION  

    Helicobacter pylori (H. pylori) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n = 20) but not in patients with chronic gastritis (CG; n = 20) or in healthy volunteers (HC; n = 10). This antigen was over-expressed by a stressful (heat-stressed) environment, and was identified as elongation factor 2 kinase (EF-2K) by western blotting. The GU patients&apos; lymphocytes stimulated by H. pylori specifically disrupted heat-stressed HGC-27 cells in a cytotoxic assay. In flow cytometry, the effector cells (lymphocytes) from GU patients were significantly differentiated to T helper type 1 lymphocyte (Th1) and cytotoxic T lymphocyte (CTL) as opposed to those from CG patients. The target cells (HGC-27) expressed EF-2K and MHC-class I together with costimulatory molecules from heat stress. This antigen specific immune mechanism could have a prominent role in the pathogenesis of GU. Copyright (C) 2009 Kiyoshi Ayada et al.

    DOI: 10.1155/2009/850623

    Web of Science

    researchmap

  • Effects of the PsbP knockdown on the photosynthetic electron transfer in Nicotiana tabacum.

    Kunio Ido, Kentaro Ifuku, Seiko Ishihara, Yumiko Yamamoto, Chikahiro Miyake, Fumihiko Sato

    Photosynthesis Energy from the Sun   5   605 - 608   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    DOI: 10.1007/978-1-4020-6709-9_136

    researchmap

  • Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory destrusor overactivity 査読

    Jae-Chul Lee, Teruhiko Yokoyama, Hyun-Jung Hwang, Hideyuki Arimitsu, Yumiko Yamamoto, Makiko Kawasaki, Tomoko Takigawa, Kouichi Takeshi, Atsushi Nishikawa, Hiromi Kumon, Keiji Oguma

    FEMS Immunology & Medical Microbiology   51 ( 1 )   201 - 211   2007年10月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-695x.2007.00301.x

    researchmap

  • Identification of structural genes for Clostridium botulinum type C neurotoxin-converting phage particles 査読

    Hyun-Jung Hwang, Jae-Chul Lee, Yumiko Yamamoto, Mahfuzur R. Sarker, Tomofusa Tsuchiya, Keiji Oguma

    FEMS Microbiology Letters   270 ( 1 )   82 - 89   2007年5月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-6968.2007.00653.x

    researchmap

  • C Terminal Half Fragment (50kDa) of Heavy Chain Components of Clostridium botulinum Type C and D Neurotoxins Can Be Used as an Effective Vaccine

    Lee Jae-Chul, Hwang Hyun-Jung, Sakaguchi Yoshihiko, YAMAMOTO Yumiko, ARIMITSU Hideyuki, TSUJI Takao, WATANABE Toshihiro, OHYAMA Tohru, TSUCHIYA Tomofusa, OGUMA Keiji

    Microbiology and immunology   51 ( 4 )   445 - 455   2007年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Center for Academic Pub. Japan  

    DOI: 10.1111/j.1348-0421.2007.tb03919.x

    CiNii Article

    CiNii Books

    researchmap

  • Helicobacter pylori heat-shock protein 60 induces interleukin-8 via a Toll-like receptor (TLR)2 and mitogen-activated protein (MAP) kinase pathway in human monocytes 査読

    Ying Zhao, Kenji Yokota, Kiyoshi Ayada, Yumiko Yamamoto, Tomayuki Okada, Lianhua Shen, Keiji Oguma

    Journal of Medical Microbiology   56 ( 2 )   154 - 164   2007年2月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    Previous reports have indicated that <italic>Helicobacter pylori</italic> heat-shock protein 60 (<italic>H. pylori</italic>-HSP60), as an immunodominant antigen, induces interleukin (IL)-8 production in human monocytes. The exact mechanism by which <italic>H. pylori</italic>-HSP60 induces IL-8 production in monocytes has not been fully elucidated. In the present study, the downstream pathway by which <italic>H. pylori</italic>-HSP60 induces IL-8 secretion in human monocytic cell lines was investigated. Intact <italic>H. pylori</italic>, heat-killed <italic>H. pylori</italic> and <italic>H. pylori</italic> recombinant HSP60 (rHpHSP60) all induced the secretion of IL-8 and the activation of mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK) and p38, but not c-Jun N-terminal kinase (JNK), up to 24 h in NOMO1 cells. The specific inhibitors PD98059 and U0126 (for ERK1/2 signalling) and SB203580 (for p38 MAPK signalling) down-regulated IL-8 secretion from rHpHSP60-treated NOMO1 cells. An anti-Toll-like receptor (TLR)2 antibody or TLR2 small interfering RNA (siRNA) partially inhibited the secretion of IL-8, and anti-TLR2 antibody also suppressed activation of ERK and p38 MAPK in rHpHSP60-treated NOMO1 cells. These reactions were associated with nuclear factor-<italic>κ</italic>B (NF-<italic>κ</italic>B)-mediated transcriptional activation, since U0126, SB203580 and the anti-TLR2 antibody decreased NF-<italic>κ</italic>B activation. Taken together, the results suggest that ERK and p38 MAPK signalling linked to the TLR2 recognition receptor in human monocytes may be an important pathway in <italic>H. pylori</italic>-HSP60-induced IL-8 secretion.

    DOI: 10.1099/jmm.0.46882-0

    researchmap

  • Effect of the PsbP knockdown on the photosynthetic electron transfer in higher plants 査読

    Ido Kunio, Ifuku Kentaro, Yamamoto Yumiko, Sato Fumihiko

    PLANT AND CELL PHYSIOLOGY   48   S98   2007年

  • PsbP Protein, But Not PsbQ Protein, Is Essential for the Regulation and Stabilization of Photosystem II in Higher Plants 査読

    Kentaro Ifuku, Yumiko Yamamoto, Taka-aki Ono, Seiko Ishihara, Fumihiko Sato

    Plant Physiology   139 ( 3 )   1175 - 1184   2005年11月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    <title>Abstract</title>
    PsbP and PsbQ proteins are extrinsic subunits of photosystem II (PSII) and participate in the normal function of photosynthetic water oxidation. Both proteins exist in a broad range of the oxygenic photosynthetic organisms; however, their physiological roles in vivo have not been well defined in higher plants. In this study, we established and analyzed transgenic tobacco (Nicotiana tabacum) plants in which the levels of PsbP or PsbQ were severely down-regulated by the RNA interference technique. A plant that lacked PsbQ showed no specific phenotype compared to a wild-type plant. This suggests that PsbQ in higher plants is dispensable under the normal growth condition. On the other hand, a plant that lacked PsbP showed prominent phenotypes: drastic retardation of growth, pale-green-colored leaves, and a marked decrease in the quantum yield of PSII evaluated by chlorophyll fluorescence. In PsbP-deficient plant, most PSII core subunits were accumulated in thylakoids, whereas PsbQ, which requires PsbP to bind PSII in vitro, was dramatically decreased. PSII without PsbP was hypersensitive to light and rapidly inactivated when the repair process of the damaged PSII was inhibited by chloramphenicol. Furthermore, thermoluminescence studies showed that the catalytic manganese cluster in PsbP-deficient leaves was markedly unstable and readily disassembled in the dark. The present results demonstrated that PsbP, but not PsbQ, is indispensable for the normal PSII function in higher plants in vivo.

    DOI: 10.1104/pp.105.068643

    Scopus

    PubMed

    J-GLOBAL

    researchmap

  • Post-translational regulation of CND41 protease activity in senescent tobacco leaves

    Yusuke Kato, Yumiko Yamamoto, Shinya Murakami, Fumihiko Sato

    Planta   222 ( 4 )   643 - 651   2005年11月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00425-005-0011-4

    Scopus

    PubMed

    researchmap

    その他リンク: http://link.springer.com/article/10.1007/s00425-005-0011-4/fulltext.html

  • Structure and function of the PsbP protein of Photosystem II from higher plants 査読

    Kentaro Ifuku, Toru Nakatsu, Ren Shimamoto, Yumiko Yamamoto, Seiko Ishihara, Hiroaki Kato, Fumihiko Sato

    Photosynthesis Research   84 ( 1-3 )   251 - 255   2005年6月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s11120-004-7160-3

    PubMed

    researchmap

    その他リンク: http://link.springer.com/article/10.1007/s11120-004-7160-3/fulltext.html

  • Functional analysis of four members of the PsbP family in photosystem II in Nicotiana tabacum using differential RNA interference 査読

    Ishihara S, Yamamoto Y, Ifuku K, Sato F

    Plant and Cell Physiology   46 ( 12 )   1885 - 1893   2005年

     詳細を見る

    記述言語:英語   出版者・発行元:Japanese Society of Plant Physiologists  

    DOI: 10.1093/pcp/pci207

    Scopus

    PubMed

    CiNii Article

    CiNii Books

    J-GLOBAL

    researchmap

    その他リンク: http://orcid.org/0000-0001-9497-4452

  • Functional analysis of the four PsbP members encoded by a multigene family in Nicotiana tabacum.

    Seiko Ishihara, Yumiko Yamamoto, Kentaro Ifuku, Fumihiko Sato

    Photosynthesis fundamental aspects to global perspectives   2005年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    DOI: 10.14841/jspp.2005.0.012.0

    researchmap

  • The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco 査読

    Yusuke Kato, Shinya Murakami, Yumiko Yamamoto, Hiroshi Chatani, Yoshihiko Kondo, Takeshi Nakano, Akiho Yokota, Fumihiko Sato

    Planta   220 ( 1 )   97 - 104   2004年11月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00425-004-1328-0

    Scopus

    PubMed

    J-GLOBAL

    researchmap

    その他リンク: http://link.springer.com/article/10.1007/s00425-004-1328-0/fulltext.html

  • Specific RNA Interference in psbP Genes Encoded by a Multigene Family in Nicotiana tabacumwith a Short 3′-Untranslated Sequence 査読

    Kentaro IFUKU, Yumiko YAMAMOTO, Fumihiko SATO

    Bioscience, Biotechnology, and Biochemistry   67 ( 1 )   107 - 113   2003年1月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1271/bbb.67.107

    researchmap

  • A sequential two-step proteolytic process in the carboxyl-terminal truncation of precursor D1 protein in Synechocystis sp. PCC68031 査読

    Noritoshi Inagaki, Yumiko Yamamoto, Kimiyuki Satoh

    FEBS Letters   509 ( 2 )   197 - 201   2001年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1016/s0014-5793(01)03180-5

    researchmap

  • Overexpression and Characterization of Carboxyl-terminal Processing Protease for Precursor D1 Protein 査読

    Yumiko Yamamoto, Noritoshi Inagaki, Kimiyuki Satoh

    Journal of Biological Chemistry   276 ( 10 )   7518 - 7525   2001年3月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1074/jbc.m008877200

    researchmap

  • Possible involvement of two-step processing in the C-terminal cleavage of precursor D1 protein in Synechocystis sp. PCC6803.

    稲垣言要

    PS2001 Proceedings   S5   022   2001年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    researchmap

  • Some aspects of carboxyl-terminal processing of precursor D1 protein in photosystem II.

    稲垣言要

    PS2001 Proceedings   S5   023   2001年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    researchmap

  • Competitive inhibition analysis of the enzyme-substrate interaction in the carboxy-terminal processing of the precursor D1 protein of photosystem II reaction center using substituted oligopeptides 査読

    Yumiko Yamamoto, Kimiyuki Satoh

    FEBS Letters   430 ( 3 )   261 - 265   1998年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1016/s0014-5793(98)00671-1

    researchmap

  • Processing of precursor D1 protein in the PSII membrane by CtpA over-expressed in E-coli - An analysis using an antibody which specifically recognizes the cleavage product. 査読

    Y Yamamoto, K Satoh

    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V   3131 - 3134   1998年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:SPRINGER  

    Web of Science

    researchmap

  • Genetic Engineering of the Processing Site of D1 Precursor Protein of Photosystem II Reaction Center in Chlamydomonas reinhardtii

    Y. Takahashi, K. Utsumi, Y. Yamamoto, A. Hatano, K. Satoh

    Plant and Cell Physiology   37 ( 2 )   161 - 168   1996年3月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1093/oxfordjournals.pcp.a028927

    researchmap

  • Carboxyl-terminal processing protease for the D1 precursor protein: cloning and sequencing of the spinach cDNA

    Noritoshi Inagaki, Yumiko Yamamoto, Hitoshi Mori, Kimiyuki Satoh

    Plant Molecular Biology   30 ( 1 )   39 - 50   1996年1月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/bf00017801

    researchmap

    その他リンク: http://link.springer.com/article/10.1007/BF00017801/fulltext.html

  • Identification of the Carboxyl-Terminal Processing Protease for the D1 Precursor Protein of the Photosystem II Reaction Center of Spinach 査読

    稲垣言要

    Plant and Cell Physiology   36   1169 - 1177   1995年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    researchmap

  • Recognition of the Structure around the Site of Cleavage by the Carboxyl-terminal Processing Protease for D1 Precursor Protein of the Photosystem II Reaction Center 査読

    Fumiko Taguchi, Yumiko Yamamoto, Kimiyuki Satoh

    Journal of Biological Chemistry   270 ( 18 )   10711 - 10716   1995年5月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1074/jbc.270.18.10711

    researchmap

  • Carboxyl-terminal processing protease for D1 precursor protein in spinach.

    稲垣言要

    Photosynthesis: from Light to Biosphere   3   783 - 786   1995年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    researchmap

  • Recognition signal for the C-terminal processing protease of D1 precursor protein in the photosystem II reaction center. An analysis using synthetic oligopeptides 査読

    Fumiko Taguchi, Yumiko Yamamoto, Noritoshi Inagaki, Kimiyuki Satoh

    FEBS Letters   326 ( 1-3 )   227 - 231   1993年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1016/0014-5793(93)81796-3

    researchmap

▼全件表示

MISC

  • 細菌性コラゲナーゼのコラーゲン・アンカーの構造活性相関と歯周病治療への応用

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Caviness Perry, Sakon Joshua, 内田 健太郎, 中村 心, 岡本 健太郎, 高柴 正悟

    日本細菌学雑誌   75 ( 1 )   138 - 138   2020年1月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本細菌学会  

    researchmap

  • 細菌性コラゲナーゼのマトリックス・アンカーの構造解析と骨新生誘導のための複合剤開発(Structural analysis of a matrix anchor in bacterial collagenase to develop an osteogenic therapeutic)

    松下 治, 内田 健太郎, 関口 裕之, 美間 健彦, 後藤 和義, 山本 由弥子, 横田 憲治, 高相 晶士, Bauer Ryan, Sakon Joshua

    日本細菌学雑誌   72 ( 1 )   109 - 109   2017年2月

     詳細を見る

    記述言語:英語   出版者・発行元:日本細菌学会  

    researchmap

  • C型ボツリヌス毒素変換ファージのゲノム比較と遺伝子機能解析(The genome sequence of Clostridium botulinum type C phage and functional analysis of gene products)

    阪口 義彦, 内山 淳平, 小椋 義俊, 山本 由弥子, 松崎 茂展, 林 哲也, 松下 治, 小熊 惠二, 林 俊治

    日本細菌学雑誌   71 ( 1 )   135 - 135   2016年2月

     詳細を見る

    記述言語:英語   出版者・発行元:日本細菌学会  

    researchmap

  • 合成コラーゲン様基剤とコラーゲン結合型線維芽細胞増殖因子を用いた複合剤による骨形成促進法の開発

    濱本 奈々, 内田 健太郎, 関口 裕之, 美間 健彦, 後藤 和義, 山本 由弥子, 横田 憲治, 高相 晶士, 松下 治

    日本細菌学雑誌   71 ( 1 )   126 - 126   2016年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本細菌学会  

    researchmap

  • 神経障害性疼痛へのボツリヌス毒素の効果

    松香芳三, 丸濵功太郎, 三宅愛, 山本由弥子, 前川賢治, 古味佳子, 園山亘, 小熊惠二, 窪木拓男

    歯界展望   65 ( 5 )   666 - 666   2013年

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本歯科医師会  

    researchmap

  • BOTULINUM TOXIN TYPE A (150 kDa) DECREASES EXAGGERATED NEUROTRANSMITTER RELEASE FROM TRIGEMINAL GANGLION NEURONS AND RELIEVES NEUROPATHY BEHAVIORS INDUCED BY INFRAORBITAL NERVE CONSTRICTION (vol 159, pg 1422, 2009)

    Y. Kitamura, Y. Matsuka, I. Spigelman, Y. Ishihara, Y. Yamamoto, W. Sonoyama, H. Kamioka, T. Yamashiro, T. Kuboki, K. Oguma

    NEUROSCIENCE   161 ( 3 )   950 - 950   2009年7月

     詳細を見る

    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/j.neuroscience.2009.04.067

    Web of Science

    researchmap

  • 三叉神経因性疼痛モデルラットにおける改良A型ボツリヌス毒素の効果

    北村 洋一, 松香 芳三, 石原 嘉人, 園山 亘, 山本 由弥子, 小熊 恵二, 窪木 拓男

    日本口腔顔面痛学会雑誌   1 ( 1 )   69 - 69   2008年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔顔面痛学会  

    J-GLOBAL

    researchmap

  • Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory detrusor overactivity

    Keiji Oguma, Jae-Chul Lee, Teruyuki Yokoyama, Yumiko Yamamoto, Yoshihiko Sakaguchi, Shaobo Ma, Hideyuki Arimitsu

    TOXICON   51   41 - 41   2008年6月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/j.toxicon.2008.04.122

    Web of Science

    researchmap

  • Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory destrusor overactivity (vol 51, pg 201, 2007)

    Jae-Chul Lee, Teruhiko Yokoyama, Hyun-Jung Hwang, Hideyuki Arimitsu, Yumiko Yamamoto, Makiko Kawasaki, Tomoko Takigawa, Kouichi Takeshi, Atsushi Nishikawa, Hiromi Kumon, Keiji Oguma

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   51 ( 3 )   587 - 587   2007年12月

     詳細を見る

    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    DOI: 10.1111/j.1574-695X.2007.00351.x

    Web of Science

    researchmap

  • Effects of the PsbP knockdown on the photosynthetic electron transfer in Nicotiana tabacum.

    K. Ido, K. Ifuku, S. Ishihara, Y. Yamamoto, C. Miyake, F. Sato

    PHOTOSYNTHESIS RESEARCH   91 ( 2-3 )   192 - 192   2007年2月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:SPRINGER  

    Web of Science

    researchmap

  • Structures and functional differentiation of the extrinsic subunits of photosystem II

    K Ifuku, Y Yamamoto, F Sato

    PLANT AND CELL PHYSIOLOGY   47   S9 - S9   2006年

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

    Web of Science

    researchmap

  • Down-regulation of psbO, psbP and psbQ genes in Nicotiana tabacum by RNA interference technique

    K Ifuku, Y Yamamoto, S Ishihara, F Sato

    PLANT AND CELL PHYSIOLOGY   46   S27 - S27   2005年

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

    Web of Science

    researchmap

  • Functional analysis of the psbP gene family in Nicotiana tabacum

    S Ishihara, Y Yamamoto, K Ifuku, F Sato

    PLANT AND CELL PHYSIOLOGY   45   S80 - S80   2004年

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

    Web of Science

    researchmap

  • Suppression of psbP genes in Nicotiana tabacum by RNAi

    Y Yamamoto, K Ifuku, S Ishihara, F Sato

    PLANT AND CELL PHYSIOLOGY   45   S221 - S221   2004年

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

    Web of Science

    researchmap

  • Processing of CND41 might be important for the activation of protease activity

    Y Kato, Y Yamamoto, F Sato

    PLANT AND CELL PHYSIOLOGY   45   S59 - S59   2004年

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

    Web of Science

    researchmap

▼全件表示

 

担当授業科目

  • 基礎病態演習 (2022年度) 特別  - その他

  • 感染症と戦う (2022年度) 第2学期  - 月1~2

  • 病原細菌学I(演習・実習) (2022年度) 特別  - その他

  • 病原細菌学I(講義・演習) (2022年度) 特別  - その他

  • 病原細菌学II(演習・実習) (2022年度) 特別  - その他

  • 病原細菌学II(講義・演習) (2022年度) 特別  - その他

  • 細菌学 (2022年度) 特別  - その他

  • 細菌学実習 (2022年度) 特別  - その他

  • 感染症と戦う (2021年度) 第2学期  - 木1~2

  • 病原細菌学I(演習・実習) (2021年度) 特別  - その他

  • 病原細菌学I(講義・演習) (2021年度) 特別  - その他

  • 病原細菌学II(演習・実習) (2021年度) 特別  - その他

  • 病原細菌学II(講義・演習) (2021年度) 特別  - その他

  • 細菌学 (2021年度) 特別  - その他

  • 細菌学実習 (2021年度) 特別  - その他

  • 感染症と戦う (2020年度) 第2学期  - 木1,木2

  • 病原細菌学I(演習・実習) (2020年度) 特別  - その他

  • 病原細菌学I(講義・演習) (2020年度) 特別  - その他

  • 病原細菌学II(演習・実習) (2020年度) 特別  - その他

  • 病原細菌学II(講義・演習) (2020年度) 特別  - その他

  • 細菌学 (2020年度) 特別  - その他

  • 細菌学実習 (2020年度) 特別  - その他

▼全件表示