Updated on 2025/12/15

写真a

 
YAMAMOTO Yumiko
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Assistant Professor
Position
Assistant Professor
External link

Degree

  • (BLANK) ( Okayama University )

  • (BLANK) ( Okayama University )

Research Interests

  • Clostridium botulinum

  • Neurotoxin

  • 神経毒素

  • ボツリヌス菌

Research Areas

  • Life Science / Bacteriology

  • Life Science / Molecular biology

  • Life Science / Functional biochemistry

 

Papers

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MISC

  • 抗ブドウ球菌エンドライシンのリンカー領域が機能に与える影響(Functional impact by shorting a linker region of a staphylococcal endolysin)

    宗友 荘介, 内山 淳平, 内山 伊代, Thamonwan Wanganuttara, 津久井 利広, 萩谷 英大, 山本 由弥子, 神田 秀幸, 松下 治

    日本細菌学雑誌   79 ( 2 )   265 - 265   2024.7

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  • 胃炎-胃癌患者の胃粘膜より分離された硝酸塩還元菌の性状

    桑木 星里香, 山本 由弥子, 内山 淳平, 松下 治, 後藤 和義, 渡辺 朱理, 横田 憲治

    日本細菌学雑誌   79 ( 2 )   152 - 152   2024.6

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  • 胃癌患者の胃内より分離した硝酸塩還元菌のピロリ菌共感染マウスへの影響

    小松原 万里奈, 山本 由弥子, 内山 淳平, 松下 治, 後藤 和義, 渡辺 朱理, 横田 憲治

    日本細菌学雑誌   79 ( 2 )   140 - 140   2024.6

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  • 抗ブドウ球菌エンドライシンのリンカー領域が機能に与える影響

    宗友 荘介, 内山 淳平, 内山 伊代, Thamonwan Wanganuttara, 津久井 利広, 萩谷 英大, 山本 由弥子, 神田 秀幸, 松下 治

    日本細菌学雑誌   79 ( 2 )   119 - 119   2024.6

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  • Functional impact by linker region of a staphylococcal endolysin

    宗友荘介, 内山淳平, 内山伊代, THAMONWAN Wanganuttara, 津久井利広, 萩谷英大, 山本由弥子, 神田秀幸, 松下治

    日本細菌学雑誌(Web)   79 ( 2 )   2024

  • Characteristics of nitrate-reducing bacteria from patients with gastritis and gastric cancer

    桑木星里香, 山本由弥子, 内山淳平, 松下治, 後藤和義, 渡辺朱理, 横田憲治

    日本細菌学雑誌(Web)   79 ( 2 )   2024

  • Effects of nitrate-reducing bacteria from the gastric cancer patients in H. pylori co-infected mice

    小松原万里奈, 山本由弥子, 内山淳平, 松下治, 後藤和義, 渡辺朱理, 横田憲治

    日本細菌学雑誌(Web)   79 ( 2 )   2024

  • Implications for bacterial evolution of giant phages with uracil-containing DNA genomes

    内山淳平, 内山伊代, 後藤和義, 山本由弥子, 松崎茂展, 松下治

    日本細菌学雑誌(Web)   78 ( 1 )   2023

  • Genomic analysis of neurotoxin-converting phages of Clostridium botulinum types C and D

    阪口義彦, 武晃, 後藤和義, 山本由弥子, 幸田知子, 向本雅郁, 小崎俊司, 林俊治, 林哲也, 小熊惠二

    日本細菌学雑誌(Web)   78 ( 1 )   2023

  • Analysis of pseudolysogenic lifecycle of Clostridium botulinum types C and D phages

    阪口義彦, 武晃, 後藤和義, 山本由弥子, 幸田知子, 向本雅郁, 小崎俊司, 林俊治, 林哲也, 小熊惠二

    日本細菌学雑誌(Web)   77 ( 1 )   2022

  • 敗血症性肺塞栓症患者から分離されたTsukamurella inchonensisの同定(Identification of Tsukamurella inchonensis isolated from septic pulmonary emboli(SPE) patient)

    I Putu Bayu Mayura, Gotoh Kazuyoshi, 美間 健彦, 山本 由弥子, 横田 憲治, 松下 治, 萩谷 英大

    日本細菌学雑誌   76 ( 1 )   89 - 89   2021.2

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  • 細菌性コラゲナーゼのコラーゲン・アンカーの構造活性相関と歯周病治療への応用

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Caviness Perry, Sakon Joshua, 内田 健太郎, 中村 心, 岡本 健太郎, 高柴 正悟

    日本細菌学雑誌   75 ( 1 )   138 - 138   2020.1

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  • 細菌性コラゲナーゼのコラーゲン・アンカーと歯周組織再生への応用(Collagen anchors of bacterial collagenases and their application to periodontal tissue regeneration)

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Perry Caviness, Sakon Joshua, 小出 隆規, 内田 健太郎, 中村 心, 高柴 正悟

    日本細菌学雑誌   74 ( 1 )   84 - 84   2019.3

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  • C型とD型ボツリヌス毒素変換ファージの解析(Analysis of botulinum neurotoxin-converting phages in Clostridium botulinum types C and D)

    阪口 義彦, 内山 淳平, 小椋 義俊, 後藤 和義, 山本 由弥子, 松崎 茂展, 山口 明日美, 林 哲也, 小熊 惠二, 林 俊治

    日本細菌学雑誌   73 ( 1 )   94 - 94   2018.2

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  • 細菌性コラゲナーゼのPKDドメインの構造機能解析と骨新生誘導剤の開発(Structure analysis of bacterial collagenases to develop therapeutics to induce osteogenesis)

    松下 治, 内田 健太郎, 美間 健彦, 後藤 和義, 山本 由弥子, 横田 憲治, Bauer Ryan, 高相 晶士, Sakon Joshua

    日本細菌学雑誌   73 ( 1 )   114 - 114   2018.2

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  • C型とD型ボツリヌス毒素変換ファージの解析(Analysis of botulinum neurotoxin-converting phages in Clostridium botulinum types C and D)

    阪口 義彦, 内山 淳平, 小椋 義俊, 後藤 和義, 山本 由弥子, 松崎 茂展, 山口 明日美, 林 哲也, 小熊 惠二, 林 俊治

    日本細菌学雑誌   73 ( 1 )   94 - 94   2018.2

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  • 細菌性コラゲナーゼのマトリックス・アンカーの構造解析と骨新生誘導のための複合剤開発(Structural analysis of a matrix anchor in bacterial collagenase to develop an osteogenic therapeutic)

    松下 治, 内田 健太郎, 関口 裕之, 美間 健彦, 後藤 和義, 山本 由弥子, 横田 憲治, 高相 晶士, Bauer Ryan, Sakon Joshua

    日本細菌学雑誌   72 ( 1 )   109 - 109   2017.2

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  • Vibrio alginolyticusのコラゲナーゼ発現はHapRにより調節される(Expression of colA is regulated by HapR in Vibrio alginolyticus)

    美間 健彦, 西川 裕太郎, 中田 悠介, 波多野 直哉, 後藤 和義, 山本 由弥子, 横田 憲治, 松下 治

    日本細菌学雑誌   72 ( 1 )   106 - 106   2017.2

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  • C型ボツリヌス毒素変換ファージのゲノム比較と遺伝子機能解析(The genome sequence of Clostridium botulinum type C phage and functional analysis of gene products)

    阪口 義彦, 内山 淳平, 小椋 義俊, 山本 由弥子, 松崎 茂展, 林 哲也, 松下 治, 小熊 惠二, 林 俊治

    日本細菌学雑誌   71 ( 1 )   135 - 135   2016.2

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  • 合成コラーゲン様基剤とコラーゲン結合型線維芽細胞増殖因子を用いた複合剤による骨形成促進法の開発

    濱本 奈々, 内田 健太郎, 関口 裕之, 美間 健彦, 後藤 和義, 山本 由弥子, 横田 憲治, 高相 晶士, 松下 治

    日本細菌学雑誌   71 ( 1 )   126 - 126   2016.2

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  • Vibrio alginolyticus I.029のコラゲナーゼ発現はHapRにより調節される

    西川 裕太郎, 美間 健彦, 中田 悠介, 後藤 和義, 山本 由弥子, 横田 憲治, 松下 治

    日本細菌学雑誌   71 ( 1 )   127 - 127   2016.2

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  • Clostridium argentinenseにおけるG型ボツリヌス神経毒素遺伝子をコードするプラスミドの分析(Analysis of botulinum neurotoxin type G gene-encoding plasmid in Clostridium argentinense)

    阪口 義彦, 内山 淳平, 鈴木 智典, 山本 由弥子, 小椋 義俊, 細見 晃司, 松崎 茂展, 林 哲也, 小崎 俊司, 小熊 惠二

    日本細菌学雑誌   69 ( 1 )   236 - 236   2014.2

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  • C型ボツリヌス毒素変換ファージのコアゲノムの探索

    阪口義彦, 内山淳平, 小椋義俊, 山本由弥子, 鈴木智典, 松崎茂展, 林哲也, 小熊惠二

    日本細菌学雑誌   68 ( 1 )   178 - 178   2013.2

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  • 神経障害性疼痛へのボツリヌス毒素の効果

    松香芳三, 丸濵功太郎, 三宅愛, 山本由弥子, 前川賢治, 古味佳子, 園山亘, 小熊惠二, 窪木拓男

    歯界展望   65 ( 5 )   666 - 666   2013

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  • BOTULINUM TOXIN TYPE A (150 kDa) DECREASES EXAGGERATED NEUROTRANSMITTER RELEASE FROM TRIGEMINAL GANGLION NEURONS AND RELIEVES NEUROPATHY BEHAVIORS INDUCED BY INFRAORBITAL NERVE CONSTRICTION (vol 159, pg 1422, 2009)

    Y. Kitamura, Y. Matsuka, I. Spigelman, Y. Ishihara, Y. Yamamoto, W. Sonoyama, H. Kamioka, T. Yamashiro, T. Kuboki, K. Oguma

    NEUROSCIENCE   161 ( 3 )   950 - 950   2009.7

  • 三叉神経痛治療のための薬物治療法の開発

    松香芳三, 北村洋一, 山本由弥子, 園山 亘, Spigelman I, 小熊恵二, 窪木拓男

    日本歯科医学会誌   28 ( 1 )   49 - 53   2009

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  • 三叉神経因性疼痛モデルラットにおける改良A型ボツリヌス毒素の効果

    北村 洋一, 松香 芳三, 石原 嘉人, 園山 亘, 山本 由弥子, 小熊 恵二, 窪木 拓男

    日本口腔顔面痛学会雑誌   1 ( 1 )   69 - 69   2008.12

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  • Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory detrusor overactivity

    Keiji Oguma, Jae-Chul Lee, Teruyuki Yokoyama, Yumiko Yamamoto, Yoshihiko Sakaguchi, Shaobo Ma, Hideyuki Arimitsu

    TOXICON   51   41 - 41   2008.6

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    DOI: 10.1016/j.toxicon.2008.04.122

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  • The effect of peripheral administration of botulinum toxin type A in rats with neuropathic pain

    Yoshizo Matsuka, Yoichi Kitamura, Igor Spigelman, Rahena Akther, Yumiko Yamamoto, Wataru Sonoyama, Keiji Oguma, Takuo Kuboki

    TOXICON   51   7 - 7   2008.6

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    DOI: 10.1016/j.toxicon.2008.04.020

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  • Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory destrusor overactivity (vol 51, pg 201, 2007)

    Jae-Chul Lee, Teruhiko Yokoyama, Hyun-Jung Hwang, Hideyuki Arimitsu, Yumiko Yamamoto, Makiko Kawasaki, Tomoko Takigawa, Kouichi Takeshi, Atsushi Nishikawa, Hiromi Kumon, Keiji Oguma

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   51 ( 3 )   587 - 587   2007.12

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  • Effects of the PsbP knockdown on the photosynthetic electron transfer in Nicotiana tabacum.

    K. Ido, K. Ifuku, S. Ishihara, Y. Yamamoto, C. Miyake, F. Sato

    PHOTOSYNTHESIS RESEARCH   91 ( 2-3 )   192 - 192   2007.2

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  • Structures and functional differentiation of the extrinsic subunits of photosystem II

    K Ifuku, Y Yamamoto, F Sato

    PLANT AND CELL PHYSIOLOGY   47   S9 - S9   2006

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  • Down-regulation of psbO, psbP and psbQ genes in Nicotiana tabacum by RNA interference technique

    K Ifuku, Y Yamamoto, S Ishihara, F Sato

    PLANT AND CELL PHYSIOLOGY   46   S27 - S27   2005

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  • Suppression of psbP genes in Nicotiana tabacum by RNAi

    Y Yamamoto, K Ifuku, S Ishihara, F Sato

    PLANT AND CELL PHYSIOLOGY   45   S221 - S221   2004

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  • Processing of CND41 might be important for the activation of protease activity

    Y Kato, Y Yamamoto, F Sato

    PLANT AND CELL PHYSIOLOGY   45   S59 - S59   2004

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  • Functional analysis of the psbP gene family in Nicotiana tabacum

    S Ishihara, Y Yamamoto, K Ifuku, F Sato

    PLANT AND CELL PHYSIOLOGY   45   S80 - S80   2004

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  • CARBOXYL-TERMINAL EXTENSION OF PRECURSOR D1 PROTEIN IS EXCISED WITH TWO STEPS BY CTPA IN SYNECHOCYSTIS 6803 :

    INAGAKI Noritoshi, YAMAMOTO Yumiko, SATOH Kimiyuki

    Plant and cell physiology   42   s171   2001

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    Other Link: https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=185133

  • セネデスムスLF-1株を用いたD1タンパク質C-末プロセシングの解析

    山本 由弥子, 稲垣 言要, 佐藤 公行

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   61   194 - 194   1997.9

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  • 光化学系II反応中心D1タンパク質のC末端切断酵素の部位特異的突然変異による触媒中心の解析

    稲垣 言要, PAKRASI Himadri, 山本 由弥子, 佐藤 公行

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   61   206 - 206   1997.9

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  • ホウレンソウD1タンパク質前駆体C-末切断酵素の大腸菌における発現

    山本 由弥子, 松本 哲, 稲垣 言要, 佐藤 公行

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   60   214 - 214   1996.10

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Research Projects

  • Preclinical study on botulinum toxin therapy in treatment for neuropathic pain

    Grant number:17K01358  2017.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMAMOTO Yumiko

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    We have previously shown that botulinum toxin type A (BoNT/A) alleviates trigeminal neuropathy induced by infraorbital nerve constriction. In this study, we generated two rat models of chemotherapy-induced peripheral neuropathy, and assessed the analgesic effect of BoNT/A in chemotherapy-induced neuropathy. Unilateral BoNT/A administration into the whisker pad area attenuated chemotherapy-induced mechanical allodynia bilaterally.
    In addition, we investigated the cleavage of synaptobrevin and syntaxin in the trigeminal ganglion neurons by botulinum toxin type B (BoNT/B) and by botulinum toxin type C (BoNT/C) respectively, to apply BoNT/B and BoNT/C to treatment for neuropathic pain.

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  • Neural transmission mechanisms of tooth mechanical stimulation -Development of treatment for occlusal dysesthesia patients-

    Grant number:26293412  2014.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    MATSUKA Yoshizo

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    Grant amount:\16120000 ( Direct expense: \12400000 、 Indirect expense:\3720000 )

    Rat premolar teeth were mechanically stimulated and the active part of the brain was measured by PET using FDG. Rats under anesthesia stimulated the right maxillary permolar while measuring the stimulation strength using an electric von Frei device. Before the stimulation of the teeth, FDG was injected from a tail vein and stimulated with a strength of 100, 200, 300 g. The brain PET image was divided into 4 parts (upper right, upper left, lower right, lower left) and the peak value of FDG accumulation was measured. As a result, the stimulation at 300 g showed more FDG accumulation than the 100 or 200 g stimulation. In addition, there were more FDG accumulation was observed in the trigeminal ganglion and the sensory part when the brain was classified into the sensory area, the motor area, hippocampus, trigeminal ganglion and spinal cord.

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  • Clarification of molecular mechanisms underlying the analgesic effect of botulinum toxin and applied study

    Grant number:26350499  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMAMOTO YUMIKO, MATSUSHITA Osamu, OGUMA Keiji

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    We developed an assay to measure the proteolytic activity of botulinum toxin (BoNT), and attempted to synthesize fluorescently labeled full-length BoNT, in order to study molecular mechanisms for the analgesic effect of BoNT. We also generated four kinds of anti-peptide antibodies against SNAP-25, and showed that BoNT/A or BoNT/E cleaves SNAP-25 in cultured trigeminal ganglion neurons. This suggests that peripherally administered BoNT decreases the release of neurotransmitters via the proteolysis of SNARE proteins in sensory ganglion neurons and relieves neuropathic pain.

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  • Development of neuro-regeneration with Botulinum toxin

    Grant number:23659897  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    MATSUKA Yoshizo, KUBOKI Takuo, MATSUO Ryuji, OGUMA Keiji, YAMAMOTO Yumiko, KUMADA Ai

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    Grant amount:\3510000 ( Direct expense: \2700000 、 Indirect expense:\810000 )

    Dissociated rat trigeminal ganglion somata were incubated with matrigel in 37℃ CO_2condition. Commercially available hippocampus neuron was used in this study. Neuronal change was observed and the neuron that incubated with type A Botulinum toxin was survived longer than control. Also, dendrite of the neuron with Rotulinum toxin was longer than control neuron.

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  • Development of a new treatment for trigeminal neuron sensitization

    Grant number:22390365  2010.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    MATSUKA Yoshizo, KUBOKI Takuo, YAMAMOTO Yumiko, KUMADA Ai, OGUMA Keiji

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    Grant amount:\18720000 ( Direct expense: \14400000 、 Indirect expense:\4320000 )

    Fluorescein labeled botulinum toxin heavy chain which works for endocytosis was injected into rat face skin and found in ipsilateral trigeminal ganglion. The labeled botulinum toxin heavy chain was not found on the contralateral side or in the only fluorescein injection. The uptake was inhibited by colchicine treatment, which blocks axonal transport. Intradermal injection of botulinum toxin alleviates infraorbital nerve constriction induced thermal hyperalgesia in an operant assay. Direct application of botulinum toxin to dorsal root ganglia reversed the sciatic nerve entrapment induced decreases in withdrawal thresholds.

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  • The development of novel drug delivery systems based on the mechanism for intestinal absorption of botulinum toxins

    Grant number:22500427  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMAMOTO Yumiko, OGUMA Keiji

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    In food-borne botulism, the orally ingested botulinum neurotoxins, which could be classified into seven types (A to G) based on their antigenicity, must be absorbed from the small intestine. In this study, we produced the liposomes modified with HA, which is a non-toxic component of the type A botulinum toxin complex, in order to develop novel intestine-targeted drug delivery systems. On the other hand, we showed that the type E botulinum toxin might bind to the intestinal cells via the Hc domain of neurotoxin.

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  • Establishment of new procedure for botulism including bioterrorism, and application of botulinum toxin to the treatment

    Grant number:19390126  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    OGUMA Keiji, YOKOTA Kenji, AYADA Kiyoshi, SAKAGUCHI Yoshihiko, YAMAMOTO Yumiko, ARIMITSU Hideyuki

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    Grant amount:\19110000 ( Direct expense: \14700000 、 Indirect expense:\4410000 )

    ボツリヌス菌や毒素、抗毒素抗体の検出方法と毒素の治療への応用を検討した。菌の検出法としては、A~F型毒素遺伝子を増幅できるPCRを開発した。毒素の検出法としてはイムノクロマト法を開発したが、現在、改良中である。抗体の検出法としては、毒素の一部を結合させたカラムを用いて抗体を濃縮して検出する方法を開発した。治療への応用としては、三叉神経痛と前立腺肥大症に適用できることをとラットを用いて実証した。

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  • Basic mechanisms of nociceptive sensitization of first order sensory neuron in trigeminal neuralgia and treatment development

    Grant number:18390512  2006 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    MATSUKA Yoshizo, MAEKAWA Kenji, KUBOKI Takuo, KANYAMA Manabu, SUGIMOTO Tomosada, TAKEI Koji, ONO Tsuyoshi, OGUMA Keiji, YAMAMOTO Yomiko, KITAMURA Yoichi, KUMADA Ai, SPIGELMAN Igor, NEUBERT John k.

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    Grant amount:\16850000 ( Direct expense: \14300000 、 Indirect expense:\2550000 )

    We found that trigeminal nerve constriction induced facial pain and increased neurotransmitter release from trigeminal ganglion cell body. Also, facial injection of highly purified Botulinum toxin type A 150 kDa (BoNT/A/A) decreased the pain level and the neurotransmitter release.
    These results made clear the mechanisms of trigeminal neuralgia and peripheral BoNT/A injection may be an important treatment in the near future.

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