2021/12/16 更新

写真a

スギモト マナブ
杉本 学
SUGIMOTO Manabu
所属
資源植物科学研究所 准教授
職名
准教授
外部リンク

学位

  • 修士(薬学) ( 京都薬科大学 )

  • 博士(農学) ( 京都大学 )

研究キーワード

  • Environmental stress

  • functional biomolecule

  • space environment

  • 環境ストレス

  • 機能物質

  • 宇宙環境

研究分野

  • ライフサイエンス / 機能生物化学

  • ライフサイエンス / ゲノム生物学

  • ライフサイエンス / システムゲノム科学

学歴

  • 京都大学    

    - 1989年

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    国名: 日本国

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  • 京都大学   Graduate School, Division of Agriculture  

    - 1989年

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  • 京都薬科大学   Faculty of Pharmaceutical Science  

    - 1983年

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  • 京都薬科大学   Pharmaceutical Sciences   Pharmaceutical Chemistry

    - 1983年

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    国名: 日本国

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経歴

  • Visiting Professor,Kazan Federal University, Russia

    2014年 - 2015年

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  • ロシア連邦カザン大学 客員教授

    2014年 - 2015年

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  • - Associate Professor,Institute of Plant Science and Resources,Okayama University

    2004年

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  • - 岡山大学資源植物科学研究所 准教授

    2004年

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  • 米国ミズーリ−・コロンビア大学 客員研究員

    1990年 - 1992年

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  • visiting researcher,Missouri-Columbia University, U.S.A.

    1990年 - 1992年

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▼全件表示

所属学協会

委員歴

  • おかやまバイオアクティブ研究会   企画委員  

    2021年4月 - 2022年3月   

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    団体区分:学協会

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  • 日本宇宙生物科学会   代議員  

    2021年4月 - 2022年3月   

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    団体区分:学協会

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論文

  • Anti-Inflammatory Effect on Colitis and Modulation of Microbiota by Fermented Plant Extract Supplementation

    Manabu Sugimoto, Toshiro Watanabe, Motoko Takaoka, Kyoko Suzuki, Tadatoshi Murakami, Nobutada Murakami, Shoichi Sumikawa

    Fermentation   7 ( 2 )   55 - 55   2021年4月

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    担当区分:筆頭著者, 責任著者   出版者・発行元:MDPI AG  

    Although results of recent studies suggest that fermented foods strongly affect the gut microbiota composition and that they relieve inflammatory bowel disease symptoms, some reports have described that fermented foods increase some inflammation markers based on differences in fermented food materials. This study evaluated the effects of fermented plant extract (FPE) on dextran sulfate sodium (DSS)-induced colitis in mice and the effects on fecal microbiota composition in humans. Mice fed 5% FPE with 3% DSS (FPE group) showed no body weight loss, atrophy of colonic length, or bloody stool, similar to mice fed a basal diet (negative group), whereas mice fed 3% DSS (positive group) exhibited those effects. Concentrations of inflammation markers IL-6 and TNF-α were not significantly different between FPE and negative groups; however, those concentrations became higher in the positive group. 16S ribosomal RNA gene sequencing was used to characterize fecal microbiota in healthy women before and after 3-month FPE supplementation. The FPE supplementation induced increases in Firmicutes phyla and in Clostridiales order, which play a central role in inflammation suppression. These results suggest that FPE enhances Clostridiales growth in the gut and that it has an anti-inflammatory effect.

    DOI: 10.3390/fermentation7020055

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  • Transcriptomic analysis of developing seeds in a wheat (<i>Triticum aestivum</i> L.) mutant RSD32 with reduced seed dormancy

    Kazuhide Rikiishi, Manabu Sugimoto, Masahiko Maekawa

    Breeding Science   71 ( 2 )   155 - 166   2021年

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    出版者・発行元:Japanese Society of Breeding  

    DOI: 10.1270/jsbbs.20016

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  • Transformation of Major Ginsenosides into Minor Ginsenosides in Ginseng by Pickling in Salted Rice Malt Paste 査読

    Manabu Sugimoto, Nobutada Murakami

    Advances in Nutrition & Food Science   5   27 - 32   2020年5月

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    担当区分:筆頭著者, 責任著者   記述言語:英語  

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  • 宇宙環境による作物の生存能力と遺伝子発現への影響 招待 査読

    杉本 学

    BSJ-Review   11   75 - 82   2020年2月

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    担当区分:筆頭著者, 責任著者   記述言語:日本語  

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  • LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice

    Wan-Yi Chiou, Tadafumi Kawamoto, Eiko Himi, Kazuhide Rikiishi, Manabu Sugimoto, Mika Hayashi-Tsugane, Kazuo Tsugane, Masahiko Maekawa

    Plant and Cell Physiology   60 ( 3 )   503 - 515   2019年3月

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    出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1093/pcp/pcz014

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  • Rice Salt-Tolerance Enhancement by Expression of 1-Aminocyclopropane-1- Carboxylic Acid Oxidase Gene from Salt Tolerant Barley 査読

    Sugimoto M, Houjyo Y, Maekawa M, Terada R

    Journal of Plant Biochemistry & Physiology   06 ( 04 )   2018年

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    担当区分:責任著者   出版者・発行元:OMICS Publishing Group  

    DOI: 10.4172/2329-9029.1000226

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  • Structure and Molecular Characterization of Diadenosine Polyphosphate Hydrolase in Brachypodium distachyon 査読

    Motohiro Tanaka, Igor Iamshchikov, Yusuke Kato, Rushan Sabirov, Oleg Gusev, Wataru Sakamoto, Manabu Sugimoto

    Journal of Plant Biochemistry & Physiology   06 ( 03 )   2018年

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    担当区分:責任著者   出版者・発行元:OMICS Publishing Group  

    DOI: 10.4172/2329-9029.1000220

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  • Planetary protection challenges in space exploration missions and ways of their resolution with account of Russian exobiology experiments

    O. I. Orlov, N. D. Novikova, N. A. Polikarpov, M. A. Levinskikh, E. A. Deshevaya, M. Sugimoto, V. R. Alekseev, T. Okuda, O. A. Gusev, V. N. Sychev

    REACH   6   25 - 33   2017年6月

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    記述言語:英語   出版者・発行元:Elsevier GmbH.  

    DOI: 10.1016/j.reach.2017.07.001

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  • Molecular characterization of barley methionine γ-lyase and expression by abiotic stress and aspartate family amino acids.

    Sugimoto, M, Tanaka, H, Murakami, N

    J. Plant Biochem. Physiol.   5 ( 3 )   199   2017年

  • Gene expression of rice seeds surviving 13- and 20-month exposure to space environment

    Manabu Sugimoto, Youko Oono, Yoshihiro Kawahara, Oleg Gusev, Masahiko Maekawa, Takashi Matsumoto, Margarita Levinskikh, Vladimir Sychev, Natalia Novikova, Anatoly Grigoriev

    Life Sciences in Space Research   11   10 - 17   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ltd  

    Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96% respectively. Those for 20 months were 7 and 76%, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.

    DOI: 10.1016/j.lssr.2016.10.001

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  • Structure and molecular characterization of barley nudix hydrolase genes

    Sayuri Tanaka, Makoto Kihara, Manabu Sugimoto

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   79 ( 3 )   394 - 401   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Putative nudix hydrolase (NUDX) genes, which encode amino acid sequences showing homology with those of Arabidopsis NUDXs and conserve nudix motif, were identified from barley. The 14 deduced barley NUDXs (HvNUDX1-14) were classified into established subfamilies, except for 8-oxo-deoxyguanosine 5 '-triphosphate (8-oxo-dGTP) pyrophosphohydrolase and mRNA decapping enzyme subfamilies, and three substrate-unknown subfamilies. Drought and UV-C stresses, respectively, up-regulated 7 and 4 HvNUDX genes, but some homologs of Arabidopsis NUDXs showed different responses to abiotic stress. HvNUDX12 gene, belonging to diadenosine tetraphosphates (Ap(4)A) pyrophosphohydrolase subfamily gene and up-regulated by UV-C, was expressed in Escherichia coli cells. The recombinant protein showed 8-oxo-dGTP, Ap(4)A, and guanosine-3 ',5 '-tetraphosphate (ppGpp) pyrophosphohydrolase activities, and the suppression of the lacZ amber mutation in a mutT-deficient E. coli cells caused by the incorporation of 8-oxo-GTP into mRNA was prevented to a significant degree. These results suggest that barley NUDXs have unique constitution and response of NUDX to abiotic stress.

    DOI: 10.1080/09168451.2014.978259

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  • Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight

    Manabu Sugimoto, Youko Oono, Oleg Gusev, Takashi Matsumoto, Takayuki Yazawa, Margarita A. Levinskikh, Vladimir N. Sychev, Gail E. Bingham, Raymond Wheeler, Mary Hummerick

    BMC PLANT BIOLOGY   14 ( 4 )   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Spaceflight environment have been shown to generate reactive oxygen species (ROS) and induce oxidative stress in plants, but little is known about the gene expression of the ROS gene network in plants grown in long-term spaceflight. The molecular response and adaptation to the spaceflight environment of Mizuna plants harvested after 27 days of cultivation onboard the International Space Station (ISS) were measured using genome-wide mRNA expression analysis (mRNA-Seq).
    Results: Total reads of transcripts from the Mizuna grown in the ISS as well as on the ground by mRNA-Seq showed 8,258 and 14,170 transcripts up-regulated and down-regulated, respectively, in the space-grown Mizuna when compared with those from the ground-grown Mizuna. A total of 20 in 32 ROS oxidative marker genes were up-regulated, including high expression of four hallmarks, and preferentially expressed genes associated with ROS-scavenging including thioredoxin, glutaredoxin, and alternative oxidase genes. In the transcription factors of the ROS gene network, MEKK1-MKK4-MPK3, OXI1-MKK4-MPK3, and OXI1-MPK3 of MAP cascades, induction of WRKY22 by MEKK1-MKK4-MPK3 cascade, induction of WRKY25 and repression of Zat7 by Zat12 were suggested. RbohD and RbohF genes were up-regulated preferentially in NADPH oxidase genes, which produce ROS.
    Conclusions: This large-scale transcriptome analysis revealed that the spaceflight environment induced oxidative stress and the ROS gene network activation in the space-grown Mizuna. Among transcripts altered in expression by space conditions, some were common genes response to abiotic and biotic stress. Furthermore, certain genes were exclusively up-regulated in Mizuna grown on the ISS. Surprisingly, Mizuna grew in space normally, as well as on the ground, demonstrating that plants can acclimate to long-term exposure in the spaceflight environment by reprogramming the expression of the ROS gene network.

    DOI: 10.1186/1471-2229-14-4

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  • Viability of barley seeds after long-term exposure to outer side of international space station

    Manabu Sugimoto, Makoto Ishii, Izumi C. Mori, Shagimardanova Elena, Oleg A. Gusev, Makoto Kihara, Takehiro Hoki, Vladimir N. Sychev, Margarita A. Levinskikh, Natalia D. Novikova, Anatoly I. Grigoriev

    ADVANCES IN SPACE RESEARCH   48 ( 6 )   1155 - 1160   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (SO generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (SI generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the SO and SI generation. Furthermore, the SI and S2 space-stored seeds respectively showed similar beta-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine SO space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space. (C) 2011 COSPAR. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.asr.2011.05.017

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  • Survival of dormant organisms after long-term exposure to the space environment

    N. Novikova, O. Gusev, N. Polikarpov, E. Deshevaya, M. Levinskikh, V. Alekseev, T. Okuda, M. Sugimoto, V. Sychev, A. Grigoriev

    ACTA ASTRONAUTICA   68 ( 9-10 )   1574 - 1580   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The RF SRC-Institute of Biomedical Problems, Russian Academy of Sciences, developed Biorisk hardware to study the effects of long-term exposure of dormant forms of various organisms to outer space and used it to complete a series of experiments on the Russian Module (RM) of the International Space Station (ISS).
    The experiments were performed using prokaryotes (Bacillus bacteria) and eukaryotes (Penicillium, Aspergillus, and Cladosporium fungi), as well as spores, dormant forms of higher plants, insects, lower crustaceans, and vertebrates. The biological samples were housed in two containers that were exposed to outer space for 13 or 18 months. The results of the 18-month experiment showed that, in spite of harsher temperature than in the first study, most specimens remained viable.
    These experiments provided evidence that not only bacterial and fungal spores but also dormant forms of organisms that reached higher levels of evolutionary development had the capability to survive a long-term exposure to outer space. This observation suggests that they can be transferred on outer walls of space platforms during interplanetary missions. (C) 2010 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.actaastro.2010.05.019

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  • Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment

    E. I. Shagimardanova, O. A. Gusev, V. N. Sychev, M. A. Levinskikh, M. R. Sharipova, O. N. Il&apos;inskaya, G. Bingham, M. Sugimoto

    MOLECULAR BIOLOGY   44 ( 5 )   734 - 740   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MAIK NAUKA/INTERPERIODICA/SPRINGER  

    The transcriptome of barley Hordeum vulgare grown aboard the International Space Station was studied using microarray analysis. In the spaceflight environment, mRNA levels of over 500 genes were changed more than twofold; among them, genes of stress response proteins, in particular, heat shock proteins, pathogenesis-related proteins, and antioxidant proteins. Further analysis by real-time PCR confirmed enhanced transcription of reactive oxygen species scavenging genes. The superoxide dismutase (sod) mRNA level in the space environment was 6-fold higher than in earth conditions. The transcript levels of glutamyl transferase (gst), catalase (cat), and ascorbate peroxidase (apx) were increased in spaceflight 24, 18, and 3 times in comparison to ground control, respectively. For the first time, it has been shown that spaceflight environment can induce oxidative stress in plants.

    DOI: 10.1134/S0026893310050080

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  • Oxidative Stress and Antioxidant Capacity in Barley Grown under Space Environment

    Elena Shagimardanova, Oleg Gusev, Gail E. Bingham, Margarita A. Levinskikh, Vladimir N. Sychev, Zhou Tiansu, Makoto Kihara, Kazutoshi Ito, Manabu Sugimoto

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 7 )   1479 - 1482   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    The gene expression and enzyme activity of superoxide dismutase, catalase, and ascorbate peroxidase in the space-grown barley were not significantly different from those of the ground-grown barley. Cu2+ reducing and radical scavenging activities in an extract of the space-grown barley were lower than those of the ground-grown barley by 0.7 fold, suggesting that the space environment does not induce oxidative stress, and reduces antioxidant capacity in plants.

    DOI: 10.1271/bbb.100139

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  • Proteomic Analysis of Specific Proteins in the Root of Salt-Tolerant Barley

    Manabu Sugimoto, Kazuyoshi Takeda

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   73 ( 12 )   2762 - 2765   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Comparative two-dimensional electrophoresis showed six proteins, which were significantly produced in the root of salt-tolerant barley. These proteins were identified as stress/defense-related proteins that do not scavenge reactive oxygen species directly, suggesting that salt-tolerant barley develops not only an antioxidative system, but also physical and biochemical changes to cope with salt stress.

    DOI: 10.1271/bbb.90456

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  • The biorisk experiment: 13-month exposure of resting forms of organism on the outer side of the russian segment of the international space station: Preliminary results

    V. M. Baranov, N. D. Novikova, N. A. Polikarpov, V. N. Sychev, M. A. Levinskikh, V. R. Alekseev, T. Okuda, M. Sugimoto, O. A. Gusev, A. I. Grigor'Ev

    Doklady Biological Sciences   426 ( 1 )   267 - 270   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1134/S0012496609030223

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  • Localization and expression of serine racemase in Arabidopsis thaliana

    Manabu Sugimoto, Wataru Sakamoto, Yoshiyuki Fujitani

    AMINO ACIDS   36 ( 3 )   587 - 590   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with beta-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.

    DOI: 10.1007/s00726-008-0112-z

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  • 09安全で安心な遺伝子組換え植物作製のための新規選択マーカー系の開発

    杉本学

    生物学に関する試験研究論叢   24   93 - 97   2009年

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  • Development of a new selection marker for production of safe transgenic plants

    Sugimoto, M

    24   93 - 97   2009年

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  • Space Educational Experiment "Space Silkworm"

    Sugimoto, M, h-grade students, a, Higashiune Elementary School, Gusev, O, Ryazanskii, S, Illyin, E, Orlov, O

    Space Utilization Research   24   355 - 356   2008年

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  • Gene expression of barley grown in space

    Sugimoto, M, Shagimardanova, E, Gusev, O, Levinskikh, M, Sychev, V, Grigoriev, A

    Space Utilization Research   24   412 - 414   2008年

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  • Differential changes in cell wall matrix polysaccharides and glycoside-hydrolyzing enzymes in developing wheat seedlings differing in drought tolerance

    Haruyoshi Konno, Yoshiki Yamasaki, Manabu Sugimoto, Kazuyoshi Takeda

    JOURNAL OF PLANT PHYSIOLOGY   165 ( 7 )   745 - 754   2008年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    The growth kinetics and variations in cell wait matrix polysaccharides and glycoside hydrolases during seedling development of the drought-tolerant wheat cultivar (cv. Hong Mang Mai) were compared with the drought-sensitive cultivar (cv. Shirasagi-komugi). After 15d of culture in water at 22 degrees C under constant irradiance of 98 mu mol m(-2) s(-1), the length of the coleoptile and leaf sheath of Hong Mang Mai seedlings was 1.7 times longer than those of Shirasagikomugi seedlings. In the cell watts isolated from coleoptiles and Leaf sheaths of the seedling of the two cultivars, the contents of arabinose, xylose, and glucose changed during development. The cell walls were fractionated progressively with 50 mM CDTA, 50 mM Na(2)CO(3), 1M KOH and 4M KOH, and sugar composition was determined. The amount of CDTA-soluble fraction from the Hong Mang Mai cell walls was 2.4-fold higher than that from the Shirasagikomugi cell walls at 6d of culture, and a considerable decrease was observed during development. The ratio of arabinose to xylose in 1M KOH-soluble fraction from the two cultivars decreased. The amount of 4M KOH-soluble fraction from the Shirasagikomugi cell watts was affected much more than those of the Hong Mang Mai cell watts. Many glycoside hydrolase activities were detected in the protein fractions from coleoptiles and leaf sheaths of the two cultivars, and the activities of licheninase, 1,3-1,4-beta-glucanase, and 1,3-beta-glucanase in the LiCl-soluble protein fraction increased drastically during development of the Shirasagikomugi seedlings. These findings suggest that the metabolism of the cell wall matrix polysaccharides of the drought-tolerant wheat cultivar is far different from that of the drought-sensitive wheat cultivar during seedling development. (c) 2007 Elsevier GmbH. All rights reserved.

    DOI: 10.1016/j.jplph.2007.07.007

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  • Serine racemases from barley, Hordeum vulgare L., and other plant species represent a distinct eukaryotic group: Gene cloning and recombinant protein characterization

    Yoshiyuki Fujitani, Terumi Horiuchi, Kazutoshi Ito, Manabu Sugimoto

    PHYTOCHEMISTRY   68 ( 11 )   1530 - 1536   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Several D-amino acids have been identified in plants. However, the biosynthetic pathway to them is unclear. In this study, we cloned and sequenced a cDNA encoding a serine racemase from barley which contained an open reading frame encoding 337 amino acid residues. The deduced amino acid sequence showed significant identity to plant and mammalian serine racemases and contained conserved pyridoxal 5-phosphate (PLP)-binding lysine and PLP-interacting amino acid residues. The purified gene product catalyzed not only racemization of serine but also dehydration of serine to pyruvate. The enzyme requires PLP and divalent cations such as Ca2+, Mg2+, or Mn2+, but not ATP, whereas mammalian serine racemase activity is increased by ATP. In addition to the results regarding the effect of ATP on enzyme activity and the phylogenetic analysis of eukaryotic serine racemases, the antiserum against Arabidopsis serine racemase did not form a precipitate with barley and rice serine racemases. This suggests that plant serine racemases represent a distinct group in the eukaryotic serine racemase family and can be clustered into monocot and dicot types. (c) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.phytochem.2007.03.040

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  • Perspectives of RNA/DNA studies using lattent stages of invertebrates and plants exposed to space flight and outer space environment.

    Gusev, O, Sychev, V, Levinskikh, M, Sugimoto, M

    Space Utiliz. Res.   23   344 - 346   2007年

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  • Molecular and biochemical characterization of a serine racemase from Arabidopsis thaliana

    Y Fujitani, N Nakajima, K Ishihara, T Oikawa, K Ito, M Sugimoto

    PHYTOCHEMISTRY   67 ( 7 )   668 - 674   2006年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    A cDNA encoding a homolog of mammalian serine racemase, a unique enzyme in eukaryotes, was isolated from Arabidopsis thaliana and expressed in Escherichia coli cells. The gene product, of which the amino acid residues for binding pyridoxal 5'-phosphate (PLP) are conserved in this as well as mammalian serine racemases, catalyzes not only serine racemization but also dehydration of serine to pyruvate. The enzyme is a homodimer and requires PLP and divalent cations, Ca2+, Mg2+, Mn2+, Fe2+, or Ni2+, at alkaline pH for both activities. The racemization process is highly specific toward L-serine, whereas L-alanine, L-arginine, and L-glutamine were poor substrates. The V-max/K-m values for racemase activity of L- and D-serine are 2.0 and 1.4 nmol/mg/min/mM, respectively, and those values for L- and D-serine on dehydratase activity are 13 and 5.3 nmol/mg/min/mM, i.e. consistent with the theory of racemization reaction and the specificity of dehydration toward L-serine. Hybridization analysis showed that the serine racemase gene was expressed in various organs of A. thaliana. (c) 2006 Elsevier Ltd. All rights reserved.

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  • An isozyme of earthworm serine proteases acts on hydrolysis of triacylglycerol

    N Nakajima, M Sugimoto, S Tsuboi, H Tsuji, K Ishihara

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   69 ( 10 )   2009 - 2011   2005年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    An enzyme catalyzing the hydrolysis of triacylglycerol was purified from an earthworm. The N-terminal amino acid sequence and the catalytic function of the purified enzyme were identical to those of Isozyme C, an isozyme of the earthworm-serine proteases. No other lipase proteins were found in the earthworm cells. The isozyme might act on the hydrolysis of triacylglycerol as well as the protein decomposition.

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  • 感染症の症状を軽減した耐病性日本産アコヤガイ系統の開発

    内村裕之, 西川 智, 浜田耕示, 兵藤勝也, 広瀬琢磨, 石原浩二, 杉本 学, 中島伸佳

    水産育種   2005年

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  • Cloning and characterization of preferentially expressed genes in an aluminum-tolerant mutant derived from Penicillium chrysogenum IF04626

    M Sugimoto, Y Saiki, DM Zhang, F Kawai

    FEMS MICROBIOLOGY LETTERS   230 ( 1 )   137 - 142   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    cDNAs expressed preferentially in an Al-tolerant microorganism were isolated by subtraction hybridization with cDNAs of Al-sensitive Penicillium chrysogenum IFO4626 as driver cDNA and cDNAs of the Al-tolerant mutant derived from the wild cells by UV irradiation as tester cDNA. Northern blot analysis revealed that mRNA levels of six genes were increased significantly in the Al-tolerant mutant after exposure to Al stress when compared with the wild cells. Two genes accumulated in both the presence and absence of Al stress and four genes were induced by Al stress in the Al-tolerant mutant. cDNA fragments were amplified by rapid amplification of cDNA ends and sequenced to obtain full-length cDNAs of the six genes. Two genes were novel or predicted ones and the others showed significant homology to known genes, ADP/ATP translocase, enolase, cysteine synthase, and glucoamylase, which are induced by environmental stresses in prokaryotic and eukaryotic cells. These enzyme activities increased in the Al-tolerant mutant when compared to those in the wild cells, showing that not only the levels of gene expression but also the levels of enzyme activities increased in the Al-tolerant mutant. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0378-1097(03)00886-3

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  • Damage to cultivated Japanese pearl oysters by oxidative stress that was related to "mass mortality"

    Y Uchimura, H Yamashita, M Kuramoto, K Ishihara, M Sugimoto, N Nakajima

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   67 ( 11 )   2470 - 2473   2003年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Increased blood-DNA breakage was observed in diseased pearl oysters. They showed significant formation of 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA), whereas the oysters that had a low mortality rate from the disease had high activity of superoxide dismutase (SOD) and low amounts of 8-OHdG and MDA. These results suggest that radical damage had occurred only in the diseased pearl oysters with the cytolysis of their haemocytes, which was related to the mass mortality of the Japanese pearl oysters.

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  • Structure and function of a phospholipid hydroperoxide glutathione peroxidase-like protein from barley

    M Sugimoto, K Takeda

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   23 ( 2-6 )   397 - 403   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A cDNA encoding barley phospholipid hydroperoxide glutathione peroxidase (PHGPX)-like protein was cloned and sequenced by the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The cDNA comprised 846 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,532 Da. The deduced amino acid sequence showed significant identity to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to produce an extra protein, which showed a molecular mass similar to the deduced one, and the clone cells were much more tolerant to NaCl stress than the host cells. (C) 2003 Elsevier B.V. All rights reserved.

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  • Structure and function of an isozyme of earthworm proteases as a new biocatalyst

    M Sugimoto, K Ishihara, N Nakajima

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   23 ( 2-6 )   405 - 409   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The amino acid sequence of the earthworm-serine protease, isozyme C, which shows not only elastase-like activity but also trypsin-like activity, was determined. The catalytic triad of the trypsin family, His, Asp, Ser, was conserved in isozyme C, but the primary substrate determinant of trypsin, Asp, was missing in isozyme C, the same as in elastase. One of the two Gly at the entrance of the substrate-binding pocket of trypsin was replaced by Val as in elastase, however, the other was replaced by Ser whereas Thr is present in elastase. Furthermore, isozyme C also showed esterase-like activity, which was applicable for the synthesis of useful substances. (C) 2003 Elsevier B.V. All rights reserved.

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  • Earthworm-serine protease: characterization, molecular cloning, and application of the catalytic functions

    N Nakajima, M Sugimoto, K Ishihara

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   23 ( 2-6 )   191 - 212   2003年9月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    An earthworm, Lumbricus rubellus, produces alkaline serine proteases that are greater than trypsins in their activity and stability. The proteases which were purified from the earthworm were composed of six isozyme proteins. Each isozyme consisted of a single polypeptide chain which was derived from the different genes. The enzymes had activity and were stable at below 60 degreesC over a wide range of pH 2-11 and were strongly resistant to organic solvents and detergents. Moreover, they retain full activity for long years at room temperature. They acted on various proteins, such as elastin as well as fibrin, and some peptides, such as beta-amyloid 1-40 and solubilized actual fibrin clots of whole blood in a rat's vena cava. They also catalyzed the hydrolysis of various esters. The cDNAs encoding the proteases were cloned and sequenced. They showed similarity to mammalian serine proteases and conserved the catalytic amino acid residues, however, neither arginine nor lysine residues were present in the autolysis region. The gene encoding the native form of an isozyme protein was expressed in Pichia pastoris to produce the active protease in the culture medium. The proteases contributed to the production of the "earthworm autolysate". The extracts of the autolysate could be used as a "peptone substitute" in media for the efficient growth of microorganisms. (C) 2003 Elsevier B.V. All rights reserved.

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  • A root-specific O-methyltransferase gene expressed in salt-tolerant barley. 国際誌

    Manabu Sugimoto, Yoshihiro Okada, Kazuhiro Sato, Kazutoshi Ito, Kazuyoshi Takeda

    Bioscience, biotechnology, and biochemistry   67 ( 5 )   966 - 72   2003年5月

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    記述言語:英語  

    A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.

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  • Transglucosylation activities of multiple forms ofα-glucosidase from spinach

    M. Sugimoto, S. Furui, K. Sasaki, Y. Suzuki

    Biosci. Biotechnol. Biochem.   67 ( 5 )   1160 - 1163   2003年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1271/bbb.67.1160

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  • Change in maltose and soluble starch-hydrolyzing activities of chimeric α-glucosidases of Mucor javanicus and Aspergillus oryzae

    M. Sugimoto, T. Ohta, F. Kawai

    Biochim. Biophys. Acta   1645 ( 1 )   1 - 5   2003年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S1570-9639(02)00525-3

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  • Further stabilization of earthworm serine protease by chemical modification and immobilization

    N Nakajima, K Ishihara, M Sugimoto, T Nakahara, H Tsuji

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   66 ( 12 )   2739 - 2742   2002年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Earthworm serine protease is more stable and is less affected by organic solvents and detergent than other proteases. However, it is inactivated, probably by autolysis, at 60degreesC or above under alkaline conditions. Further stabilization was managed by chemical modification of the enzyme with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and phenylglyoxal to protect the activity from the autolytic inactivation. Stabilization was possible also under acidic conditions, in which the stability of the enzyme was rather low, by immobilization with folded sheet mesoporous material. Thus, further stabilization of the enzyme has been achieved by chemical modification or immobilization.

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  • 塩ストレス抵抗性オオムギに関する研究 : 2. 塩ストレスで誘導されるO-methyltransferase遺伝子のクローニングと構造

    杉本 学, 岡田 吉弘, 佐藤 和広, 伊藤 一敏, 武田 和義

    育種学研究 = Breeding research   4   2002年8月

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  • 塩ストレス抵抗性オオムギに関する研究1. 塩ストレスで特異的に発現する遺伝子の解析

    杉本 学, 且原 真木, 岡田 吉弘, 佐藤 和広, 伊藤 一敏, 武田 和義

    育種学研究 = Breeding research   4 ( 1 )   2002年3月

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  • The first step in polyethylene glycol degradation by sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide

    M Sugimoto, M Tanabe, M Hataya, S Enokibara, JA Duine, F Kawai

    JOURNAL OF BACTERIOLOGY   183 ( 22 )   6694 - 6698   2001年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Several Sphingomonas spp. utilize polyethylene glycols (PEGs) as a sole carbon and energy source, oxidative PEG degradation being initiated by a dye-linked dehydrogenase (PEG-DH) that oxidizes the terminal alcohol groups of the polymer chain. Purification and characterization of PEG-DH from Sphingomonas terrae revealed that the enzyme is membrane bound. The gene encoding this enzyme (pegA) was cloned, sequenced, and expressed in Escherichia coli. The purified recombinant enzyme was vulnerable to aggregation and inactivation, but this could be prevented by addition of detergent. It is as a homodimeric protein with a subunit molecular mass of 58.8 kDa, each subunit containing 1 noncovalently bound flavin adenine dinucleotide but not Fe or Zu. PEG-DH recognizes a broad variety of primary aliphatic and aromatic alcohols as substrates. Comparison with known sequences revealed that PEG-DH belongs to the group of glucose-methanol-choline (GMC) flavoprotein oxidoreductases and that it is a novel type of flavoprotein alcohol dehydrogenase related (percent identical amino acids) to other, so far uncharacterized bacterial, membrane-bound, dye-linked dehydrogenases: alcohol dehydrogenase from Pseudomonas oleovorans (46%); choline dehydrogenase from E. coli (40%); L-sorbose dehydrogenase from Gluconobacter oxydans (38%); and 4-nitrobenzyl alcohol dehydrogenase from a Pseudomonas species (35%).

    DOI: 10.1128/JB.183.22.6694-6698.2001

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  • Molecular cloning and expression of farnesyl pyrophosphate synthase gene responsible for essential oil biosynthesis in hop (Humulus lupulus)

    Y Okada, M Sugimoto, K Ito

    JOURNAL OF PLANT PHYSIOLOGY   158 ( 9 )   1183 - 1188   2001年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:URBAN & FISCHER VERLAG  

    Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of geranyl pyrophosphate (GPP) and FPP, which are the precursors of terpenoids. such as essential oils, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). gDNA and cDNA encoding hop (Humulus lupulus L.) FPP synthase was cloned by the Inverse PCR method and the Cassette-mediated PCR method and sequenced. The deduced amino acid sequence showed a high identity to plant FPP synthases. The cloned gene was expressed in Escherichia coli cells to produce the FPP synthase. Northern blot analysis revealed that the FPP synthase gene was expressed in the lupulin gland, bract, leaf, and stem of hop and expressed more strongly In the lupulin gland than in other tissues.

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  • Molecular cloning, sequencing, and expression of cDNA encoding serine protease with fibrinolytic activity from earthworm 査読

    M Sugimoto, N Nakajima

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   65 ( 7 )   1575 - 1580   2001年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    An earthworm, Lumbricus rubellus, produces alkaline trypsin-like proteases that are greater than trypsins in their stability and strong tolerance to organic solvents. cDNAs encoding strong fibrinolytic proteases (F-III-2 and F-III-1) in the six isozymes were cloned and sequenced to study their stability-structure relationship. The cDNAs of F-III-2 and F-III-1 comprised 1011 and 973 bp and included open reading frames that encode polypeptides of 245 and 246 amino acid residues, respectively. The deduced amino acid sequences of F-III-2 and F-III-1 have 7 and 8 activation peptides in the N-termini respectively, indicating that they are translated as proenzymes and modified to active forms by posttranslational processing. They showed similarity to mammalian serine proteases and conserved the catalytic amino acid residues, however, neither arginine nor lysine residues were present in the autolysis region. The gene encoding the native form of F-III-2 was expressed in Pichia pastoris to produce and secrete the earthworm protease in the culture medium, which dissolves an artificial fibrin plate.

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  • Screening and characterization of trehalose-oleate hydrolyzing lipase

    R Ishimoto, M Sugimoto, F Kawai

    FEMS MICROBIOLOGY LETTERS   195 ( 2 )   231 - 235   2001年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Various soil samples were collected to screen the presence of microorganisms which have ability to degrade TOE. One strain (AKU-883) with good TOE degrading activity was isolated and identified as Burkholderia cepacia and the extracellular enzyme was purified to homogeneity. The purification was achieved by ultrafiltration. Super Q anion-exchange chromatography and Superdex 200HR gel-filtration in the presence of Triton X. The enzyme was purified to 85-fold. and specific activity of 4.910 kU mg protein(-1). The peak preparation on gel filtration showed a single band of 34 kDa on SDS-PAGE and native PAGE which indicate the monomeric nature of the enzyme. The pr of the enzyme was 6.3. The enzyme showed the maximum activity at pH 9 and 65 degreesC. and was stable in the range of pH 5-10 and up to 60 degreesC. Almost all the activity (92%) was kept after incubation for more than 1 week at 50 degreesC (pH 7.3). High activities remained even in water-miscible solvents such as ethanol, dimethyl formamide, diisopropyl ether, and dioxane, The N-terminal 16 amino acid residues were determined as A-N-G-Y-A-A-T-R-Y-P-I-I-L-V-G-G. which showed a consensus sequence for lipases from Burkholderia species. Thus the enzyme was concluded to be a kind of lipase. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-1097(01)00016-7

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  • Molecular cloning, sequencing, and characterization of cDNAs encoding fibrinolytic enzymes from earthworm, Lumbricus rubellus

    M. Sugimoto, N. Nakajima

    Biosci. Biotechnol. Biochem.   2001年

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  • Cloning and characterization of an alpha-glucosidase gene from spinach.

    M Sugimoto, S Furui, Y Suzuki

    FASEB JOURNAL   11 ( 9 )   A1106 - A1106   1997年7月

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    記述言語:英語   出版者・発行元:FEDERATION AMER SOC EXP BIOL  

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  • Molecular cloning and characterization of a cDNA encoding α-glucosidase from spinach

    Plant Molecular Biology   33 ( 4 )   765 - 768   1997年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1023/A:1005766003923

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  • ホウレンソウα-グルコシダーゼのcDNAクローニングと構造解析 : 酵素

    杉本 学, 古井 聡, 鈴木 幸雄

    日本農藝化學會誌   70 ( 0 )   1996年3月

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    記述言語:日本語   出版者・発行元:社団法人日本農芸化学会  

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  • Chemical modification of earthworm fibrinolytic enzyme with human serum albumin fragment and characterization of the protease as a therapeutic enzyme

    Nobuyoshi Nakajima, Kohji Ishihara, Manabu Sugimoto, Hiroyuki Sumi, Katsuhiko Mikuni, Hiroki Hamada

    Bioscience, Biotechnology and Biochemistry   60 ( 2 )   293 - 300   1996年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The strongest fibrinolytic protease (F-III-2) in the six enzyme proteins purified from earthworm, Lumhricus rubellus [N. Nakajima et al., Biosci. Biotech. Biochem., 57, 1726–1730 (1993)] has been modified chemically with fragmented human serum albumin (mol. wt., 10,000–30,000). The modified enzyme lost the antigenicity of the native enzyme and reacted with the antisera against human serum albumin, the human serum albumin fragments, and the conjugate with the native enzyme to form precipitation lines, which fused with each other. The conjugate was significantly more resistant to inactivation by protease inhibitors in rat plasma. The enzyme was a non-hemorrhagic protein and did not induce platelet aggregation. The enzyme kept potent proteolytic activity for fibrin and fibrinogen than that of human plasmin. The enzyme easily solubilized actual fibrin clots (thrombi) of whole blood induced by thrombin in a rat’s vena cava. The continuous fibrinolysis for fibrin suspension in an enzyme reactor system using the modified enzyme immobilized to oxirane-activated acrylic beads has been achieved without any inactivation of the activity at least for more than 1 month. The N-terminal amino acid sequence of the protein was also investigated and the sequence showed local similarity to those of the serine proteases such as plasmin and chymotrvpsin. © 1996, Taylor &amp
    Francis Group, LLC. All rights reserved.

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▼全件表示

MISC

  • 酸化ストレスで誘導される植物由来遺伝子の過酸化脂質消去機能の解明

    杉本 学

    旭硝子財団助成研究成果報告2001   2001年

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  • ホウレンソウ種子に存在するα-グルコシダーゼの分子型変化

    古井 聡, 杉本 学, 鈴木 幸雄

    岡山大学資源生物科学研究所報告   5 ( 1 )   1 - 9   1997年3月

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    記述言語:日本語   出版者・発行元:岡山大学資源生物科学研究所  

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  • ホウレンソウ種子α-グルコシダーゼのサブサイト構造 : 酵素

    杉本 学, 古井 聡, 鈴木 幸雄

    日本農藝化學會誌   69 ( 0 )   1995年7月

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    記述言語:日本語   出版者・発行元:社団法人日本農芸化学会  

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  • ホウレンソウ保存種子α-グルコシダーゼの精製とその性質 : 酵素

    古井 聡, 杉本 学, 鈴木 幸雄

    日本農藝化學會誌   69 ( 0 )   1995年7月

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    記述言語:日本語   出版者・発行元:社団法人日本農芸化学会  

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  • Multiple molecular forms of α-hlucosidase from spinach seeds, Spinacia oleracea L.

    Bioscience, Biotechnology, and Biochemistry   59 ( 4 )   673 - 677   1995年

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    記述言語:英語  

    DOI: 10.1271/bbb.59.673

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  • Hydrolytic action on the mixture of maltose and soluble starch by α-glucosidase from Mucor javanicus IFO4570.

    Bioscience, Biotechnology, and Biochemistry   58 ( 8 )   1535 - 1536   1994年

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    記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)  

    DOI: 10.1080/bbb.58.1535

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  • Subsite affinities of α-glucosidase from Mucor javanicus

    Bioscience, Biotechnology, and Biochemistry   57 ( 8 )   1378 - 1379   1993年

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    記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)  

    DOI: 10.1271/bbb.57.1378

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  • A simple and efficient method for the oligonucleotide-directed mutagenesis using plasmid DNA template and phosphorothioate-modified nucleotide

    Manabu Sugimoto, Nobuyoshi Esaki, Hidehiko Tanaka, Kenji Soda

    Analytical Biochemistry   179 ( 2 )   309 - 311   1989年

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    記述言語:英語  

    We have developed a simple and efficient method for oligonucleotide-directed mutagenesis with double-stranded (plasmid) DNA as a template. The template was simply and rapidly prepared by cell lysis and the following DNA denaturation with alkali. The chain elongation was performed with phosphorothioate-modified nucleotide at 37°C. After the selective digestion of original DNA with NciI and exonuclease III, the desired mutated gene was obtained at a high frequency (about 70%). © 1989.

    DOI: 10.1016/0003-2697(89)90134-6

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  • A simple and efficient method for the oligonucleotide-directed mutagenesis using plasmid DNA template and phosphorothioate-modified nucleotide

    Manabu Sugimoto, Nobuyoshi Esaki, Hidehiko Tanaka, Kenji Soda

    Analytical Biochemistry   179 ( 2 )   309 - 311   1989年

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    記述言語:英語  

    We have developed a simple and efficient method for oligonucleotide-directed mutagenesis with double-stranded (plasmid) DNA as a template. The template was simply and rapidly prepared by cell lysis and the following DNA denaturation with alkali. The chain elongation was performed with phosphorothioate-modified nucleotide at 37°C. After the selective digestion of original DNA with NciI and exonuclease III, the desired mutated gene was obtained at a high frequency (about 70%). © 1989.

    DOI: 10.1016/0003-2697(89)90134-6

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  • γ-glutamyltranspeptidase activity in a human pancreatic cancer cell line (HPC-Y1) in serum-free chemically defined medium.

    Cancer Letters   25 ( 2 )   129 - 137   1984年

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講演・口頭発表等

  • 光環境撹乱による作物の極早生化と花成制御関連遺伝子の発現

    杉本 学

    日本宇宙生物科学会第35回大会  2021年9月24日 

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    開催年月日: 2021年9月24日 - 2021年9月26日

    会議種別:口頭発表(一般)  

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  • 麹床を用いた高麗人参含有メジャージンセノサイドのマイナージンセノサイドへの変換

    杉本 学, 村上允唯

    日本農芸化学会2021年度大会  2021年 

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    開催年月日: 2021年3月18日 - 2021年3月21日

    会議種別:口頭発表(一般)  

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  • 葉緑体膜の形成と機能維持に重要なVIPP1が示す新奇ATPaseおよびGTPase活性の異なる特徴

    大西紀和, 杉本 学, 張林剛, 坂本 亘

    第62回日本植物生理学会年会  2021年 

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    開催年月日: 2021年3月14日 - 2021年3月16日

    会議種別:口頭発表(一般)  

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  • Brachypodium nudix hydrolase gene induced by UV irradiation

    Sugimoto, M, Tanaka, M, Iamshchikov, I, Kato, Y, Sakamoto, W, Gusev, O, Sabirov, R

    43rd Scientific Assembly of the Committee on Space Research  2021年 

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    開催年月日: 2021年1月28日 - 2021年2月4日

    会議種別:口頭発表(一般)  

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  • ポストたんぽぽ計画の進捗状況:主として曝露実験について

    三田肇, 矢野創, 左近樹, 小林憲正, 癸生川陽子, 横谷香織, 中川和道, 杉本学, Tetyana Milojevic, 山岸明彦, 別所義隆

    第21回宇宙科学シンポジウム  2021年1月6日 

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    開催年月日: 2021年1月6日 - 2021年1月7日

    会議種別:口頭発表(一般)  

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  • 地球生物の宇宙生存可能性検証のための短期宇宙曝露実証実験システムの構築

    横堀伸一, 鳴海一成, 時下進一, 志賀靖弘, 杉本 学, 三田 肇, 橋本博文

    第34回宇宙環境利用シンポジウム  2020年1月 

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    開催年月日: 2020年1月21日 - 2020年1月22日

    会議種別:口頭発表(一般)  

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  • 地球生物の宇宙生存可能性検証のための短期宇宙曝露実験システムの構築

    横堀伸一, 時下進一, 志賀靖弘, 鳴海一成, 杉本 学, 今井栄一, 三田 肇

    極限環境生物学会2019年度(第20回)年会  2019年 

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    開催年月日: 2019年11月16日 - 2019年11月17日

    会議種別:口頭発表(一般)  

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  • 異なる大気環境下で太陽光に曝露した大麦種子の農業形質

    杉本 学, 石井 誠, Gusev, O, Sychev, V, Levinskikh, M, ovikova, N, Grigoriev, A

    日本宇宙生物科学会第33回大会  2019年9月21日 

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    開催年月日: 2019年9月21日 - 2019年9月22日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • 宇宙環境における植物の生存能力と遺伝子発現への影響 招待

    杉本 学

    日本植物学会第83回大会  2019年9月16日 

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    開催年月日: 2019年9月15日 - 2019年9月17日

    記述言語:日本語  

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  • 宇宙空間環境下と火星地表環境下で太陽光に曝露した大麦種子の生存能力

    杉本 学, 石井 誠, Gusev, O, Sychev,V.、Levinskikh,M, Novikova, N, Grigoriev, A

    日本宇宙生物科学会第32回大会  2018年9月22日 

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    開催年月日: 2018年9月22日 - 2018年9月23日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Viability and Biological Properties of Barley Seeds under Space and Mars Environments

    Sugimoto, M, Ishii, M, Gusev, O, Sychev, V, Levinskikh, M, Novikova, N, Grigoriev, A

    42th Scientific Assembly of the Committee on Space Researc  2018年 

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    開催年月日: 2018年7月14日 - 2018年7月22日

    記述言語:英語   会議種別:口頭発表(一般)  

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  • オオムギ由来L-メチオニンγ-リアーゼの構造と機能解析

    第40回日本分子生物科学会大会  2017年 

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  • 紫外線で誘導されるミナトカモジグサ由来NUDX遺伝子の機能解析

    日本宇宙生物科学会大会第31回大会  2017年 

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  • Transcriptome Analysis of Rice Seeds after Long-term Exposure to Outside of International Space Station.

    11th Asian Microgravity Symposium  2016年 

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  • Transcriptome Analysis of Rice Seeds after Long-term Exposure to Outside of International Space Station.

    11th Asian Microgravity Symposium  2016年 

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  • 国際宇宙ステーション船外に長期間曝露したイネ種子の生存能力とトランスクリプトーム解析

    日本宇宙生物科学会大会第30回大会  2016年 

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  • 紫外線で誘導されるミナトカモジグサ由来Nudix hydrolase遺伝子

    日本宇宙生物科学会大会第29回大会  2015年 

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  • Resutls of studies on long-term exposition of dormant forms of various organisms in outer space environment.

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Tanaka, S., Kihara, M., and Sugimoto, M. Expression of nudix hydrolase genes in barley under UV irradiation

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Low GI food with barley in space foods.

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Resutls of studies on long-term exposition of dormant forms of various organisms in outer space environment.

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Tanaka, S., Kihara, M., and Sugimoto, M. Expression of nudix hydrolase genes in barley under UV irradiation

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Low GI food with barley in space foods.

    40th Scientific Assembly of the Committee on Space Research  2014年 

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  • Surviving rules: wide overlap in gene expression response to desiccation and ionizing radiation in an anhydrobiotic midge

    12th European Workshop on Astrobiology  2012年 

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  • Utilization of barley for space foods

    Bioactive Okayama 2012  2012年 

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  • 宇宙環境で生育するミズナの遺伝子発現解析

    日本農芸化学会中四国支部第32回講演会  2012年 

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  • Gene expression profile of Mizuna (Brassica rapa) grown under a space environment

    39th COSPAR Scientific Assembly  2012年 

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  • Field performance and grain content of ‘Space Barley’, grain of malting barley stored outside international space station for thirteen months

    39th COSPAR Scientific Assembly  2012年 

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  • Survival in extreme environment by “preserve-expand-specialize” strategy: lessons from comparative genomics of an anhydrobiotic midge

    39th COSPAR Scientific Assembly  2012年 

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  • Role of the barley in space foods

    39th COSPAR Scientific Assembly  2012年 

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  • Utilization of barley for space foods

    Bioactive Okayama 2012  2012年 

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  • 宇宙環境で生育するミズナの細胞壁代謝関連酵素遺伝子の発現

    日本宇宙生物科学会第26回大会  2012年 

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  • 国際宇宙ステーション船外に長期間曝露した大麦種子の生存能力

    日本宇宙生物科学会第26回大会  2012年 

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  • 血糖値の上がりにくい宇宙食に関する研究 -麦30%混合玄米の官能試験並びに血糖値測定結果-

    日本宇宙生物科学会第25回大会  2011年 

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  • Taxonomic status of Rossia palpebrosa Owen, 1834 and R> glaucopie Loven, 1846 (Cephalopoda: Sepiolida) on molecular-genetic data

    EuroCeph 2011  2011年 

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  • Effect of long-term outer space flight on barley seeds

    11th European Workshop on Astrobiology  2011年 

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  • 国際宇宙ステーション船外に曝露した大麦種子の生存能力

    日本宇宙生物科学会第25回大会  2011年 

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  • 宇宙環境で生育するミズナのストレス応答・防御遺伝子の発現

    日本宇宙生物科学会第25回大会  2011年 

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  • Brewing Performance of 'Space Barley', Grain of Malting Barley Exposed to Space

    38th COSPAR Scientific Assembly  2010年 

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  • Results of The first stage (2002-2009) of investigation of higher plants onboard RS ISS, as an element of future closed Life Support Systems

    38th COSPAR Scientific Assembly  2010年 

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  • The sleeping chionomid: an insect survived 18 months of exposure to outer space

    38th COSPAR Scientific Assembly  2010年 

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  • Viability and Biological Properties of Barley Seeds Expose to Outside of International Space Station

    38th COSPAR Scientific Assembly  2010年 

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  • Growth and gene expression of barley exposed to space.

    第26回資源生物科学国際シンポジウム  2009年 

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  • Space environment in international space station is not stressful for barley

    17th IAA Humans in Space Symposium  2009年 

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  • 宇宙大麦の特性解析II: 宇宙環境に曝露した大麦種子の後代における食品安全性試験

    日本農芸化学会2009年度大会  2009年 

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  • Space environment in international space station is not stressful for barley

    17th IAA Humans in Space Symposium  2009年 

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  • 宇宙大麦の特性解析III: 宇宙環境に曝露した大麦種子の後代より醸造されたビール「Space Barley」の特性評価

    日本育種学会第116回講演会  2009年 

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  • Oxidative Stress to Barley Grown in International Space Station

    日本宇宙生物科学会第23回大会  2009年 

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  • ACC酸化酵素遺伝子を恒常的に発現する塩ストレス抵抗性大麦におけるストレス応答・防御関連遺伝子の発現

    第31回日本分子生物学会年会・第81回日本生化学会合同大会  2008年 

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  • 宇宙環境で生育する大麦の遺伝子発現

    第24回宇宙利用シンポジウム  2008年 

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  • 宇宙教育実験「宇宙カイコ」

    第24回宇宙利用シンポジウム  2008年 

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  • Proteomics Approach for Identification of Specific Proteins in Salt Tolerant Barley

    10th International Barley Genetics Symposium  2008年 

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  • ミミズ由来のセリンプロテアーゼのリパーゼ反応について

    日本農芸化学会中四国支部第21回講演会  2008年 

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  • Expression of stress/defense-related genes in barley grown under space environment

    37th COSPAR Scientific Assembly  2008年 

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  • 宇宙環境における大麦の生育とストレス

    日本宇宙生物科学会第22回大会  2008年 

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  • 宇宙環境で生育する大麦のストレス応答・防御遺伝子の発現

    日本育種学会第114回講演会  2008年 

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  • 宇宙大麦の特性解析I-宇宙環境に曝露した大麦種子の後代における農業特性調査-

    日本育種学会第114回講演会  2008年 

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  • シロイヌナズナ由来セリンラセマーゼの局在と発現

    第30回日本分子生物学会年会  2007年 

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  • オオムギ由来セリンラセマーゼ遺伝子の構造と機能解析

    日本農芸化学会2007年度大会  2007年 

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  • オオムギ由来D-システインデスルフヒドラーゼ遺伝子の構造と機能解析

    日本農芸化学会2007年度大会  2007年 

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  • 耐乾性小麦の生育過程における細胞壁多糖の変化

    日本農芸化学会2007年度大会  2007年 

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  • オオムギ由来セリンラセマーゼの構造と機能解析

    日本ビタミン学会第59回大会  2007年 

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  • 植物由来セリンラセマーゼの構造と機能解析

    2007年度酵素・補酵素研究会  2007年 

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  • 植物のD-アミノ酸代謝酵素 -セリンラセマーゼ-

    日本農芸化学会中四国支部創立5周年記念若手研究者交流シンポジウム  2006年 

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  • 塩ストレス抵抗性オオムギで特異的に発現する遺伝子の解明

    科学技術振興機構「植物の機能と制御」第4回公開シンポジウム  2006年 

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  • 醸造オオムギの幼芽における網羅的蛋白質地図の作成

    科学技術振興機構「植物の機能と制御」第4回公開シンポジウム  2006年 

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  • 水環境とオオムギの遺伝子発現制御

    第47回日本植物生理学会年会  2006年 

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  • シロイヌナズナ由来セリンラセマーゼ遺伝子の構造と機能解析

    日本農芸化学会2006年度大会  2006年 

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  • シロイヌナズナ由来セリンラセマーゼ遺伝子の発現

    日本農芸化学会中四国支部創立5周年記念第15回講演会  2006年 

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  • シロイヌナズナ由来セリンラセマーゼ遺伝子の機能と発現

    日本ビタミン学会第58回大会  2006年 

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  • 植物のD-アミノ酸代謝酵素の構造と機能

    自然科学研究機構基礎生物学研究所部門公開セミナー  2006年 

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  • Serine racemase from Arabidopsis thaliana is a bifunctional enzyme catalyzing recemization of serine and dehydration of serine to pyruvate

    20th IUBMU International Congress of Biochemistry and Molecular Biology  2006年 

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  • Earthworm serine protease: Characterization, molecular cloning, and application of the catarytic functions.

    International Chemical Congress of Pacific Basin Societies 2005  2005年 

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  • 塩ストレス抵抗性オオムギで発現するACC酸化酵素遺伝子のクローニング

    日本農芸化学会中四国支部第11回講演会  2005年 

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  • 塩ストレス抵抗性オオムギに関する研究3.恒常的に高発現するACC酸化酵素遺伝子の機能.

    日本育種学会第107・108回講演会  2005年 

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  • 塩抵抗性オオムギ根で特異的に発現しているタンパク質のプロテオーム解析.

    日本育種学会第107・108回講演会  2005年 

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  • ストレス応答遺伝子の解析 -塩ストレス抵抗性植物作出に向けて-

    2005年度岡山大学重点プロジェクト「植物医科学」第2回シンポジウム  2005年 

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  • オオムギの網羅的タンパク質マップ構築のためのプロテオーム解析

    第28回日本分子生物学会年会  2005年 

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  • Molecular cloning and expression of cDNA encoding serine protease with high fibrinolytic activity from earthworm

    第77回日本生化学会大会  2004年 

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  • Root-specific O-methyltransferase gene expressed in salt-tolerant barley

    9th International barley genetics symposium  2004年 

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  • ストレスに打ち勝つ農作物の創製:環境遺伝子化学的アプローチ

    岡山県立大学サテライトキャンパス「9月の大学院講座」  2004年 

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  • オオムギの網羅的タンパク質マップ構築の試み

    日本育種学会第106回講演会  2004年 

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  • 塩ストレス条件下における塩抵抗性オオムギ根cDNAのEXPRESSED SEQUENCE TAG(EST)解析

    第26回日本分子生物学会年会  2003年 

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  • Penicillium chrysogenum IFO4626から誘導したアルミニウム耐性変異株で優位に発現する遺伝子の構造と機能解析

    日本農芸化学会中四国支部例会第5回講演会  2003年 

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  • ミミズプロテアーゼの精製と性質、およびその応用

    第一回貧毛類分子生物研究会  2003年 

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  • ミミズ由来プロテアーゼの構造と機能解析

    第一回貧毛類分子生物研究会  2003年 

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  • 塩ストレス条件下における塩抵抗性オオムギ根cDNAのEST解析による特異的発現遺伝子の解明

    CREST「オオムギゲノム機能の開発と制御」成果発表国際シンポジウム  2003年 

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  • 塩ストレス条件下における塩抵抗性オオムギ根cDNAのEST解析による特異的発現遺伝子の解明

    科学技術振興事業団「植物の機能と制御」第1回公開シンポジウム  2003年 

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  • Expressed sequence tag (EST) analysis of the root cDNA library from salt-tolerant barley under salt stress

    Plant & Animal Genome XI  2003年 

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  • Penicillium chrysogenum IFO4626よりUV照射で誘導されたアルミニウム耐性変異株で特異的に発現する遺伝子の解析

    第25回日本分子生物学会年会  2002年 

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  • 塩ストレス抵抗性オオムギに関する研究.1.塩ストレスで特異的に発現する遺伝子の解析

    日本育種学会第101回講演会  2002年 

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  • ストレス抵抗性オオムギに関する研究.2.塩ストレスで誘導されるO-methyltransferase遺伝子のクローニングと構造

    日本育種学会第102回講演会  2002年 

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  • 環形動物由来の新規なセリンプロテアーゼ:その触媒機能の多目的応用(その2)

    第5回生体触媒化学シンポジウム  2001年 

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  • Identification of specific genes expressed in salt tolerant barley

    Plant & Animal Genome IX  2001年 

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  • 糸状菌Mucor javanicus由来α-グルコシダーゼの基質特異性に関与する構造解析

    日本農芸化学会2001年度大会  2001年 

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  • ホップのファルネシンピロリン酸シンターゼ遺伝子のクローニングと解析

    日本育種学会第99回講演会  2001年 

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  • ポリアルキレングリコール分解微生物の分離

    日本農芸化学会2000年度大会  2000年 

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  • Cloning and expression of the polyethylene glycol dehydrogenase gene from Sphingomonas terrae

    American Society for Mocrobiology The 100th General meeting  2000年 

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  • Crude oil-emulsifying compounds produced by Alcanivorax sp. ST-T1

    American Society for Mocrobiology The 100th General meeting  2000年 

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  • Sphingomonas terrae由来ポリエチレングリコール脱水素酵素遺伝子のクローニングと構造解析

    日本農芸化学会2000年度大会  2000年 

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  • 大腸菌で発現するSphingomonas terrae由来ポリエチレングリコール脱水素酵素遺伝子の精製と性質

    日本農芸化学会2000年度大会  2000年 

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  • Alcanivorax sp. ST-T1による原油乳化性バイオサーファクタントの生産と特性

    日本農芸化学会2000年度大会  2000年 

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  • Isolation and characterization of acid and Al-tolerant Microorganisms

    日本農芸化学会2000年度大会  2000年 

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  • Isolation and characterization of aluminium-tolerant microorganisms

    日本生物工学会大会  1999年 

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  • 植物由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと構造解析

    日本農芸化学界1999年度大会  1999年 

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  • 合成高分子代謝における細菌細胞外膜機能

    日本農芸化学会1999年度大会  1999年 

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  • Structure and function of plant genes induced by oxidative stress

    Agricultural Science and Technology for the Next Generation  1999年 

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受賞

  • 日本農芸化学会中四国支部奨励賞

    2004年  

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    受賞国:日本国

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担当授業科目

  • 植物細胞分子機能学 (2021年度) 前期  - その他

  • 植物細胞分子生化学 (2021年度) 後期  - 水5~8

  • 植物細胞分子生化学演習 (2021年度) 前期  - その他

  • 植物細胞分子生化学演習 (2021年度) 後期  - その他

  • 植物細胞分子生化学演習 (2021年度) 後期  - その他

  • 植物細胞分子生化学演習 (2021年度) 前期  - その他

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 植物栄養学 (2020年度) 第4学期  - 水1,水2

  • 植物細胞分子機能学 (2020年度) 前期  - その他

  • 植物細胞分子生化学 (2020年度) 後期  - その他

  • 植物細胞分子生化学演習 (2020年度) 前期  - その他

  • 植物細胞分子生化学演習 (2020年度) 後期  - その他

  • 植物細胞分子生化学演習 (2020年度) 後期  - その他

  • 植物細胞分子生化学演習 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

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メディア報道

  • 宇宙の大麦大きく育て 児童ら種まき 新聞・雑誌

    上毛新聞  2019年12月

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  • 「宇宙を旅した大麦」収穫 新聞・雑誌

    上毛新聞  2019年6月

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  • 宇宙帰りの大麦収穫 新聞・雑誌

    上毛新聞  2019年5月

     詳細を見る