Updated on 2025/11/08

写真a

 
SUGIMOTO Manabu
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
External link

Degree

  • 修士(薬学) ( 京都薬科大学 )

  • Doctor (Agriculture) ( Kyoto University )

Research Interests

  • Environmental stress

  • functional biomolecule

  • space environment

  • 環境ストレス

  • 機能物質

  • 宇宙環境

Research Areas

  • Life Science / Functional biochemistry

  • Life Science / Genome biology

  • Life Science / System genome science

Education

  • Kyoto University   農学研究科   農芸化学

    - 1989

      More details

    Country: Japan

    researchmap

  • Kyoto University    

    - 1989

      More details

  • Kyoto Pharmaceutical University   薬学部   製薬化学科

    - 1983

      More details

    Country: Japan

    researchmap

  • Kyoto Pharmaceutical University    

    - 1983

      More details

Research History

  • Visiting Professor,Kazan Federal University, Russia

    2014 - 2015

      More details

  • ロシア連邦カザン大学 客員教授

    2014 - 2015

      More details

  • - Associate Professor,Institute of Plant Science and Resources,Okayama University

    2004

      More details

  • -

    2004

      More details

  • 米国ミズーリ−・コロンビア大学 客員研究員

    1990 - 1992

      More details

  • visiting researcher,Missouri-Columbia University, U.S.A.

    1990 - 1992

      More details

▼display all

Professional Memberships

Committee Memberships

  • 日本農芸化学会中四国支部   支部参与  

    2023.4 - 2026.3   

      More details

    Committee type:Academic society

    researchmap

  • 日本宇宙生物科学会   代議員  

    2022.9 - 2025.9   

      More details

    Committee type:Academic society

    researchmap

  • おかやまバイオアクティブ研究会   企画委員  

    2022.4 - 2026.3   

      More details

    Committee type:Academic society

    researchmap

 

Papers

  • Potential of Fermented Plant Extract for Removing Microplastics inArtificial Gastric and Intestinal Juices Reviewed

    Manabu Sugimoto, Nobutada Murakami, Maho Obayashi, Tadatoshi Murakami

    Current Nutrition & Food Science   21 ( 10 )   1085 - 1089   2025.8

     More details

    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Bentham Science Publishers Ltd.  

    Introduction:

    Microplastics (MPs) are commonly found in food and water, leading to their ingestion through daily diets. MP particles smaller than 150 µm can cross the intestinal epithelium, posing potential health risks. Rapid excretion of MPs from the intestines may help reduce these risks.

    Methods:

    Fermented plant extract (FPE) is a rich source of nutrients and active substances, which promote gut health and relieve constipation. To evaluate the MP-excluding potential of FPE, it was suspended in artificial gastric or intestinal juices, followed by the addition of 100 mg of MP particles, which remained floating in the solution. After shaking for 1 hour at 37°C, the floating MP particles were collected and weighed. The excluded weight was calculated by subtracting the weight of the floating MP particles from the initial 100 mg.

    Results:

    The weights of non-floating MP particles (diameter: 106125 µm) in artificial gastric juice mixed with 10, 5, 2.5, and 1 g of FPE were 73 ± 0.3, 74 ± 1.7, 63 ± 1.5, and 31 ± 1.5 mg, respectively. In artificial intestinal juice, the values were 45 ± 2.3, 55 ± 1.2, 43 ± 1.2, and 16 ± 2.6 mg, respectively. The weights of non-floating particles with a diameter of 250-300 µm were significantly lower than those with a diameter of 106-125 µm.

    Discussion:

    FPE exhibited greater effectiveness in acidic gastric juice, suggesting that certain MP-binding components in FPE become ionized under acidic conditions, thereby enhancing ionic interactions with microplastics. Smaller MP particles were bound more effectively than larger ones, likely due to their relatively greater surface area.

    Conclusion:

    FPE facilitates MP exclusion from the gastrointestinal tract, reducing the risk of intestinal absorption.

    DOI: 10.2174/0115734013404286250806063403

    researchmap

    Other Link: https://www.eurekaselect.com/244545/article

  • Anthocyanin can improve the survival of rice seeds from solar light outside the international space station Reviewed

    Manabu Sugimoto, Masahiko Maekawa, Hajime Mita, Shin-ichi Yokobori

    Life Sciences in Space Research   2024.10

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.lssr.2024.10.010

    researchmap

  • Blue Light-Emitting Diode Lighting Improves Antioxidant Potential in Barley Germinated Seeds Reviewed

    Manabu Sugimoto

    Journal of Plant Biochemistry & Physiology   12 ( 345 )   2024.9

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.35248/2329-9029.24.12.345

    researchmap

  • Rice Nudix Hydrolase OsNUDX2 Sanitizes Oxidized Nucleotides Reviewed

    Yuki Kondo, Kazuhide Rikiishi, Manabu Sugimoto

    Antioxidants   11 ( 9 )   1805 - 1805   2022.9

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Nudix hydrolase (NUDX) hydrolyzes 8-oxo-(d)GTP to reduce the levels of oxidized nucleotides in the cells. 8-oxo-(d)GTP produced by reactive oxygen species (ROS) is incorporated into DNA/RNA and mispaired with adenine, causing replicational and transcriptional errors. Here, we identified a rice OsNUDX2 gene, whose expression level was increased 15-fold under UV-C irradiation. The open reading frame of the OsNUDX2 gene, which encodes 776 amino acid residues, was cloned into Escherichia coli cells to produce the protein of 100 kDa. The recombinant protein hydrolyzed 8-oxo-dGTP, in addition to dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP), as did Arabidopsis AtNUDX1; whereas the amino acid sequence of OsNUDX2 had 18% identity with AtNUDX1. OsNUDX2 had 14% identity with barley HvNUDX12, which hydrolyzes 8-oxo-dGTP and diadenosine tetraphosphates. Suppression of the lacZ amber mutation caused by the incorporation of 8-oxo-GTP into mRNA was prevented to a significant degree when the OsNUDX2 gene was expressed in mutT-deficient E. coli cells. These results suggest that the different substrate specificity and identity among plant 8-oxo-dGTP-hydrolyzing NUDXs and OsNUDX2 reduces UV stress by sanitizing the oxidized nucleotides.

    DOI: 10.3390/antiox11091805

    researchmap

  • Distinctive in vitro ATP Hydrolysis Activity of AtVIPP1, a Chloroplastic ESCRT-III Superfamily Protein in Arabidopsis. International journal

    Norikazu Ohnishi, Manabu Sugimoto, Hideki Kondo, Ken-Ichi Shioya, Lingang Zhang, Wataru Sakamoto

    Frontiers in plant science   13   949578 - 949578   2022

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Vesicle-inducing protein in plastid 1 (VIPP1), characteristic to oxygenic photosynthetic organisms, is a membrane-remodeling factor that forms homo-oligomers and functions in thylakoid membrane formation and maintenance. The cyanobacterial VIPP1 structure revealed a monomeric folding pattern similar to that of endosomal sorting complex required for transport (ESCRT) III. Characteristic to VIPP1, however, is its own GTP and ATP hydrolytic activity without canonical domains. In this study, we found that histidine-tagged Arabidopsis VIPP1 (AtVIPP1) hydrolyzed GTP and ATP to produce GDP and ADP in vitro, respectively. Unexpectedly, the observed GTPase and ATPase activities were biochemically distinguishable, because the ATPase was optimized for alkaline conditions and dependent on Ca2+ as well as Mg2+, with a higher affinity for ATP than GTP. We found that a version of AtVIPP1 protein with a mutation in its nucleotide-binding site, as deduced from the cyanobacterial structure, retained its hydrolytic activity, suggesting that Arabidopsis and cyanobacterial VIPP1s have different properties. Negative staining particle analysis showed that AtVIPP1 formed particle or rod structures that differed from those of cyanobacteria and Chlamydomonas. These results suggested that the nucleotide hydrolytic activity and oligomer formation of VIPP1 are common in photosynthetic organisms, whereas their properties differ among species.

    DOI: 10.3389/fpls.2022.949578

    PubMed

    researchmap

  • Anti-Inflammatory Effect on Colitis and Modulation of Microbiota by Fermented Plant Extract Supplementation

    Manabu Sugimoto, Toshiro Watanabe, Motoko Takaoka, Kyoko Suzuki, Tadatoshi Murakami, Nobutada Murakami, Shoichi Sumikawa

    Fermentation   7 ( 2 )   55 - 55   2021.4

     More details

    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Although results of recent studies suggest that fermented foods strongly affect the gut microbiota composition and that they relieve inflammatory bowel disease symptoms, some reports have described that fermented foods increase some inflammation markers based on differences in fermented food materials. This study evaluated the effects of fermented plant extract (FPE) on dextran sulfate sodium (DSS)-induced colitis in mice and the effects on fecal microbiota composition in humans. Mice fed 5% FPE with 3% DSS (FPE group) showed no body weight loss, atrophy of colonic length, or bloody stool, similar to mice fed a basal diet (negative group), whereas mice fed 3% DSS (positive group) exhibited those effects. Concentrations of inflammation markers IL-6 and TNF-α were not significantly different between FPE and negative groups; however, those concentrations became higher in the positive group. 16S ribosomal RNA gene sequencing was used to characterize fecal microbiota in healthy women before and after 3-month FPE supplementation. The FPE supplementation induced increases in Firmicutes phyla and in Clostridiales order, which play a central role in inflammation suppression. These results suggest that FPE enhances Clostridiales growth in the gut and that it has an anti-inflammatory effect.

    DOI: 10.3390/fermentation7020055

    researchmap

  • Transcriptomic analysis of developing seeds in a wheat (Triticum aestivum L.) mutant RSD32 with reduced seed dormancy

    Kazuhide Rikiishi, Manabu Sugimoto, Masahiko Maekawa

    Breeding Science   71 ( 2 )   155 - 166   2021

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Breeding  

    DOI: 10.1270/jsbbs.20016

    researchmap

  • Transformation of Major Ginsenosides into Minor Ginsenosides in Ginseng by Pickling in Salted Rice Malt Paste Reviewed

    Manabu Sugimoto, Nobutada Murakami

    Advances in Nutrition & Food Science   5   27 - 32   2020.5

     More details

    Authorship:Lead author, Corresponding author   Language:English  

    researchmap

  • Effect of space environment on crop viability and gene expression Invited Reviewed

    11   75 - 82   2020.2

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese  

    researchmap

  • LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice

    Wan-Yi Chiou, Tadafumi Kawamoto, Eiko Himi, Kazuhide Rikiishi, Manabu Sugimoto, Mika Hayashi-Tsugane, Kazuo Tsugane, Masahiko Maekawa

    Plant and Cell Physiology   60 ( 3 )   503 - 515   2019.3

     More details

    Publisher:Oxford University Press (OUP)  

    DOI: 10.1093/pcp/pcz014

    researchmap

  • Structure and Molecular Characterization of Diadenosine Polyphosphate Hydrolase in Brachypodium distachyon Reviewed

    Motohiro Tanaka, Igor Iamshchikov, Yusuke Kato, Rushan Sabirov, Oleg Gusev, Wataru Sakamoto, Manabu Sugimoto

    Journal of Plant Biochemistry & Physiology   06 ( 03 )   2018

     More details

    Authorship:Corresponding author   Publisher:OMICS Publishing Group  

    DOI: 10.4172/2329-9029.1000220

    researchmap

  • Rice Salt-Tolerance Enhancement by Expression of 1-Aminocyclopropane-1- Carboxylic Acid Oxidase Gene from Salt Tolerant Barley Reviewed

    Sugimoto M, Houjyo Y, Maekawa M, Terada R

    Journal of Plant Biochemistry & Physiology   06 ( 04 )   2018

     More details

    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:OMICS Publishing Group  

    DOI: 10.4172/2329-9029.1000226

    researchmap

  • Planetary protection challenges in space exploration missions and ways of their resolution with account of Russian exobiology experiments

    O. I. Orlov, N. D. Novikova, N. A. Polikarpov, M. A. Levinskikh, E. A. Deshevaya, M. Sugimoto, V. R. Alekseev, T. Okuda, O. A. Gusev, V. N. Sychev

    REACH   6   25 - 33   2017.6

     More details

    Language:English   Publisher:Elsevier GmbH.  

    DOI: 10.1016/j.reach.2017.07.001

    Scopus

    researchmap

  • Molecular characterization of barley methionine γ-lyase and expression by abiotic stress and aspartate family amino acids.

    Sugimoto, M, Tanaka, H, Murakami, N

    J. Plant Biochem. Physiol.   5 ( 3 )   199   2017

  • Gene expression of rice seeds surviving 13- and 20-month exposure to space environment

    Manabu Sugimoto, Youko Oono, Yoshihiro Kawahara, Oleg Gusev, Masahiko Maekawa, Takashi Matsumoto, Margarita Levinskikh, Vladimir Sychev, Natalia Novikova, Anatoly Grigoriev

    Life Sciences in Space Research   11   10 - 17   2016.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier Ltd  

    DOI: 10.1016/j.lssr.2016.10.001

    Scopus

    PubMed

    researchmap

  • Structure and molecular characterization of barley nudix hydrolase genes

    Sayuri Tanaka, Makoto Kihara, Manabu Sugimoto

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   79 ( 3 )   394 - 401   2015.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/09168451.2014.978259

    Web of Science

    researchmap

  • Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight

    Manabu Sugimoto, Youko Oono, Oleg Gusev, Takashi Matsumoto, Takayuki Yazawa, Margarita A. Levinskikh, Vladimir N. Sychev, Gail E. Bingham, Raymond Wheeler, Mary Hummerick

    BMC PLANT BIOLOGY   14 ( 4 )   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/1471-2229-14-4

    Web of Science

    researchmap

  • Viability of barley seeds after long-term exposure to outer side of international space station

    Manabu Sugimoto, Makoto Ishii, Izumi C. Mori, Shagimardanova Elena, Oleg A. Gusev, Makoto Kihara, Takehiro Hoki, Vladimir N. Sychev, Margarita A. Levinskikh, Natalia D. Novikova, Anatoly I. Grigoriev

    ADVANCES IN SPACE RESEARCH   48 ( 6 )   1155 - 1160   2011.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.asr.2011.05.017

    Web of Science

    researchmap

  • Survival of dormant organisms after long-term exposure to the space environment

    N. Novikova, O. Gusev, N. Polikarpov, E. Deshevaya, M. Levinskikh, V. Alekseev, T. Okuda, M. Sugimoto, V. Sychev, A. Grigoriev

    ACTA ASTRONAUTICA   68 ( 9-10 )   1574 - 1580   2011.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.actaastro.2010.05.019

    Web of Science

    researchmap

  • Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment

    E. I. Shagimardanova, O. A. Gusev, V. N. Sychev, M. A. Levinskikh, M. R. Sharipova, O. N. Il'inskaya, G. Bingham, M. Sugimoto

    MOLECULAR BIOLOGY   44 ( 5 )   734 - 740   2010.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1134/S0026893310050080

    Web of Science

    researchmap

  • Oxidative Stress and Antioxidant Capacity in Barley Grown under Space Environment

    Elena Shagimardanova, Oleg Gusev, Gail E. Bingham, Margarita A. Levinskikh, Vladimir N. Sychev, Zhou Tiansu, Makoto Kihara, Kazutoshi Ito, Manabu Sugimoto

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 7 )   1479 - 1482   2010.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.100139

    Web of Science

    researchmap

  • Proteomic Analysis of Specific Proteins in the Root of Salt-Tolerant Barley

    Manabu Sugimoto, Kazuyoshi Takeda

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   73 ( 12 )   2762 - 2765   2009.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.90456

    Web of Science

    researchmap

  • The biorisk experiment: 13-month exposure of resting forms of organism on the outer side of the russian segment of the international space station: Preliminary results

    V. M. Baranov, N. D. Novikova, N. A. Polikarpov, V. N. Sychev, M. A. Levinskikh, V. R. Alekseev, T. Okuda, M. Sugimoto, O. A. Gusev, A. I. Grigor'Ev

    Doklady Biological Sciences   426 ( 1 )   267 - 270   2009.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1134/S0012496609030223

    Scopus

    PubMed

    researchmap

  • Localization and expression of serine racemase in Arabidopsis thaliana

    Manabu Sugimoto, Wataru Sakamoto, Yoshiyuki Fujitani

    AMINO ACIDS   36 ( 3 )   587 - 590   2009.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00726-008-0112-z

    Web of Science

    researchmap

  • Development of a new selection marker for production of safe transgenic plants

    Sugimoto, M

    24   93 - 97   2009

     More details

  • 09安全で安心な遺伝子組換え植物作製のための新規選択マーカー系の開発

    杉本学

    生物学に関する試験研究論叢   24   93 - 97   2009

     More details

  • Differential changes in cell wall matrix polysaccharides and glycoside-hydrolyzing enzymes in developing wheat seedlings differing in drought tolerance

    Haruyoshi Konno, Yoshiki Yamasaki, Manabu Sugimoto, Kazuyoshi Takeda

    JOURNAL OF PLANT PHYSIOLOGY   165 ( 7 )   745 - 754   2008

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jplph.2007.07.007

    Web of Science

    researchmap

  • Space Educational Experiment "Space Silkworm"

    Sugimoto, M, h-grade students, a, Higashiune Elementary School, Gusev, O, Ryazanskii, S, Illyin, E, Orlov, O

    Space Utilization Research   24   355 - 356   2008

     More details

  • Gene expression of barley grown in space

    Sugimoto, M, Shagimardanova, E, Gusev, O, Levinskikh, M, Sychev, V, Grigoriev, A

    Space Utilization Research   24   412 - 414   2008

     More details

  • Serine racemases from barley, Hordeum vulgare L., and other plant species represent a distinct eukaryotic group: Gene cloning and recombinant protein characterization

    Yoshiyuki Fujitani, Terumi Horiuchi, Kazutoshi Ito, Manabu Sugimoto

    PHYTOCHEMISTRY   68 ( 11 )   1530 - 1536   2007.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.phytochem.2007.03.040

    Web of Science

    researchmap

  • Perspectives of RNA/DNA studies using lattent stages of invertebrates and plants exposed to space flight and outer space environment.

    Gusev, O, Sychev, V, Levinskikh, M, Sugimoto, M

    Space Utiliz. Res.   23   344 - 346   2007

     More details

  • Molecular and biochemical characterization of a serine racemase from Arabidopsis thaliana

    Y Fujitani, N Nakajima, K Ishihara, T Oikawa, K Ito, M Sugimoto

    PHYTOCHEMISTRY   67 ( 7 )   668 - 674   2006.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.phytochem.2006.01.003

    Web of Science

    researchmap

  • An isozyme of earthworm serine proteases acts on hydrolysis of triacylglycerol

    N Nakajima, M Sugimoto, S Tsuboi, H Tsuji, K Ishihara

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   69 ( 10 )   2009 - 2011   2005.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.69.2009

    Web of Science

    researchmap

  • 感染症の症状を軽減した耐病性日本産アコヤガイ系統の開発

    内村裕之, 西川 智, 浜田耕示, 兵藤勝也, 広瀬琢磨, 石原浩二, 杉本 学, 中島伸佳

    水産育種   34 ( 2 )   91 - 97   2005

     More details

    Language:Japanese   Publisher:水産育種研究会  

    CiNii Article

    CiNii Books

    researchmap

  • Cloning and characterization of preferentially expressed genes in an aluminum-tolerant mutant derived from Penicillium chrysogenum IF04626

    M Sugimoto, Y Saiki, DM Zhang, F Kawai

    FEMS MICROBIOLOGY LETTERS   230 ( 1 )   137 - 142   2004.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0378-1097(03)00886-3

    Web of Science

    researchmap

  • Damage to cultivated Japanese pearl oysters by oxidative stress that was related to "mass mortality"

    Y Uchimura, H Yamashita, M Kuramoto, K Ishihara, M Sugimoto, N Nakajima

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   67 ( 11 )   2470 - 2473   2003.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.67.2470

    Web of Science

    researchmap

  • Structure and function of a phospholipid hydroperoxide glutathione peroxidase-like protein from barley

    M Sugimoto, K Takeda

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   23 ( 2-6 )   397 - 403   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S1381-1177(03000104-8

    Web of Science

    researchmap

  • Earthworm-serine protease: characterization, molecular cloning, and application of the catalytic functions

    N Nakajima, M Sugimoto, K Ishihara

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   23 ( 2-6 )   191 - 212   2003.9

     More details

  • Structure and function of an isozyme of earthworm proteases as a new biocatalyst

    M Sugimoto, K Ishihara, N Nakajima

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   23 ( 2-6 )   405 - 409   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S1381-1177(03)00105-X

    Web of Science

    researchmap

  • Comparison of Acylated Plant Pigments : Light-resistance and Radical-scavenging Ability(Biochemistry & Molecular Biology)

    NAKAJIMA Nobuyoshi, SUGIMOTO Manabu, YOKOI Hiroshi, TSUJI Hideaki, ISHIHARA Kohji

    Bioscience, biotechnology, and biochemistry   67 ( 8 )   1828 - 1831   2003.8

     More details

    Language:English   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    The acylated plant pigments synthesized by lipasecatalyzed transesterification with aromatic acids were compared in respect of their light-resistance and radicalscavenging ability. With both the flavonols and anthocyanins, their acylated derivatives were more stable against illumination with fluorescent light than their non-acylated glucosides. Their radical-scavenging ability partially decreased or was retained by acylation to the glucoside molecules.

    DOI: 10.1271/bbb.67.1828

    CiNii Article

    CiNii Books

    researchmap

    Other Link: https://jlc.jst.go.jp/DN/JALC/00230316810?from=CiNii

  • A root-specific O-methyltransferase gene expressed in salt-tolerant barley. International journal

    Manabu Sugimoto, Yoshihiro Okada, Kazuhiro Sato, Kazutoshi Ito, Kazuyoshi Takeda

    Bioscience, biotechnology, and biochemistry   67 ( 5 )   966 - 72   2003.5

     More details

    Language:English  

    A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.

    DOI: 10.1271/bbb.67.966

    PubMed

    researchmap

  • Transglucosylation activities of multiple forms of alpha-glucosidase from spinach

    M Sugimoto, S Furui, K Sasaki, Y Suzuki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   67 ( 5 )   1160 - 1163   2003.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.67.1160

    Web of Science

    researchmap

  • Change in maltose- and soluble starch-hydrolyzing activities of chimeric alpha-glucosidases of Mucor javanicus and Aspergillus oryzae

    M Sugimoto, T Ohta, F Kawai

    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS   1645 ( 1 )   1 - 5   2003.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S1570-9639(02)00525-3

    Web of Science

    researchmap

  • Further stabilization of earthworm serine protease by chemical modification and immobilization

    N Nakajima, K Ishihara, M Sugimoto, T Nakahara, H Tsuji

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   66 ( 12 )   2739 - 2742   2002.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.66.2739

    Web of Science

    researchmap

  • Studies on salt tolerance mechanisms in barley : 2. Cloning and structure of O-methyltransferase gene induced by salt stress

    SUGIMOTO M, OKADA Y, SATO K, ITO K, TAKEDA K

    育種学研究 = Breeding research   4   115 - 115   2002.8

     More details

  • Studies on salt stress-tolerant mechanism in barley.1. Analysis of genes expressed specifically under salt stress in salt stress-tolerant barley

    SUGIMOTO M, KATSUHARA M, OAKADA Y, SATO K, ITO K, TAKEDA K

    育種学研究 = Breeding research   4 ( 1 )   20 - 20   2002.3

     More details

  • The first step in polyethylene glycol degradation by sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide

    M Sugimoto, M Tanabe, M Hataya, S Enokibara, JA Duine, F Kawai

    JOURNAL OF BACTERIOLOGY   183 ( 22 )   6694 - 6698   2001.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/JB.183.22.6694-6698.2001

    Web of Science

    researchmap

  • Molecular cloning and expression of farnesyl pyrophosphate synthase gene responsible for essential oil biosynthesis in hop (Humulus lupulus)

    Y Okada, M Sugimoto, K Ito

    JOURNAL OF PLANT PHYSIOLOGY   158 ( 9 )   1183 - 1188   2001.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Molecular cloning, sequencing, and expression of cDNA encoding serine protease with fibrinolytic activity from earthworm Reviewed

    M Sugimoto, N Nakajima

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   65 ( 7 )   1575 - 1580   2001.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Screening and characterization of trehalose-oleate hydrolyzing lipase

    R Ishimoto, M Sugimoto, F Kawai

    FEMS MICROBIOLOGY LETTERS   195 ( 2 )   231 - 235   2001.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0378-1097(01)00016-7

    Web of Science

    researchmap

  • Molecular cloning, sequencing, and characterization of cDNAs encoding fibrinolytic enzymes from earthworm, Lumbricus rubellus

    M. Sugimoto, N. Nakajima

    Biosci. Biotechnol. Biochem.   2001

     More details

  • Cloning and characterization of an alpha-glucosidase gene from spinach.

    M Sugimoto, S Furui, Y Suzuki

    FASEB JOURNAL   11 ( 9 )   A1106 - A1106   1997.7

     More details

    Language:English  

    Web of Science

    researchmap

  • Molecular cloning and characterization of a cDNA encoding α-glucosidase from spinach

    Manabu Sugimoto, Satoshi Furui, Yukio Suzuki

    Plant Molecular Biology   33 ( 4 )   765 - 768   1997.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1023/A:1005766003923

    Scopus

    PubMed

    researchmap

  • ホウレンソウα-グルコシダーゼのcDNAクローニングと構造解析 : 酵素

    杉本 学, 古井 聡, 鈴木 幸雄

    日本農藝化學會誌   70 ( 0 )   290 - 290   1996.3

     More details

    Language:Japanese   Publisher:社団法人日本農芸化学会  

    CiNii Article

    CiNii Books

    researchmap

  • Chemical modification of earthworm fibrinolytic enzyme with human serum albumin fragment and characterization of the protease as a therapeutic enzyme

    Nobuyoshi Nakajima, Kohji Ishihara, Manabu Sugimoto, Hiroyuki Sumi, Katsuhiko Mikuni, Hiroki Hamada

    Bioscience, Biotechnology and Biochemistry   60 ( 2 )   293 - 300   1996.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.60.293

    Scopus

    PubMed

    researchmap

▼display all

MISC

  • [Stress response genes expression analysis of barley Hordeum vulgare under space flight environment].

    E. I. Shagimardanova, O. A. Gusev, V. N. Sychev, M. A. Levinskikh, M. R. Sharipova, O. N. Il'inskaia, G. Bingham, M. Sugimoto

    Molekuliarnaia biologiia   44   831 - 838   2010.9

  • 酸化ストレスで誘導される植物由来遺伝子の過酸化脂質消去機能の解明

    杉本 学

    旭硝子財団助成研究成果報告2001   2001

     More details

  • ホウレンソウ種子に存在するα-グルコシダーゼの分子型変化

    古井 聡, 杉本 学, 鈴木 幸雄

    岡山大学資源生物科学研究所報告   5 ( 1 )   1 - 9   1997.3

     More details

    Language:Japanese   Publisher:岡山大学資源生物科学研究所  

    CiNii Article

    CiNii Books

    researchmap

  • ホウレンソウ種子α-グルコシダーゼのサブサイト構造 : 酵素

    杉本 学, 古井 聡, 鈴木 幸雄

    日本農藝化學會誌   69 ( 0 )   199 - 199   1995.7

     More details

    Language:Japanese   Publisher:社団法人日本農芸化学会  

    CiNii Article

    CiNii Books

    researchmap

  • ホウレンソウ保存種子α-グルコシダーゼの精製とその性質 : 酵素

    古井 聡, 杉本 学, 鈴木 幸雄

    日本農藝化學會誌   69 ( 0 )   199 - 199   1995.7

     More details

    Language:Japanese   Publisher:社団法人日本農芸化学会  

    CiNii Article

    CiNii Books

    researchmap

  • Transgly-cosylation to fructose and n-ary1 glucoside by CGTase

    OKUDA K.

    Journal of the Agricultural Chemical Society of Japan   69   27 - 27   1995.7

     More details

    Language:Japanese   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    CiNii Article

    CiNii Books

    researchmap

  • Multiple Molecular Forms of α-Glucosidase from Spinach Seeds, Spinacia oleracea L

    Manabu Sugimoto, Satoshi Furui, Yukio Suzuki

    Bioscience, Biotechnology, and Biochemistry   59 ( 4 )   673 - 677   1995

     More details

  • Hydrolytic action on the mixture of maltose and soluble starch by α-glucosidase from mucor javanicus ifo 4570

    Manabu Sugimoto, Yukio Suzuki

    Bioscience, Biotechnology and Biochemistry   58 ( 8 )   1535 - 1536   1994

     More details

    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

    DOI: 10.1080/bbb.58.1535

    Scopus

    researchmap

  • Subsite Affinities of α-Glucosidase from Mucor javanicus

    Manabu Sugimoto, Yukio Suzuki

    Bioscience, Biotechnology and Biochemistry   57 ( 8 )   1378 - 1379   1993

     More details

    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

    DOI: 10.1271/bbb.57.1378

    Scopus

    researchmap

  • A simple and efficient method for the oligonucleotide-directed mutagenesis using plasmid DNA template and phosphorothioate-modified nucleotide

    Manabu Sugimoto, Nobuyoshi Esaki, Hidehiko Tanaka, Kenji Soda

    Analytical Biochemistry   179 ( 2 )   309 - 311   1989

     More details

  • GAMMA-GLUTAMYL-TRANSPEPTIDASE ACTIVITY IN A HUMAN PANCREATIC-CANCER CELL-LINE (HPC-Y1) IN SERUM-FREE CHEMICALLY DEFINED MEDIUM

    N YAMAGUCHI, M SUGIMOTO, K KAWAI

    CANCER LETTERS   25 ( 2 )   129 - 137   1984

     More details

    Language:English  

    Web of Science

    researchmap

▼display all

Presentations

  • 植物発酵エキスによるマイクロプラスチック体外排除の可能性

    杉本 学, 村上允唯, 大林真帆

    日本農芸化学会2025年度関西・中四国・西日本支部合同大会  2025.9.19 

     More details

    Event date: 2025.9.18 - 2025.9.19

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 宇宙での栽培を可能とする植物の環境適応ポテンシャル

    杉本 学

    日本宇宙生物科学会第38回大会  2024.9.21 

     More details

    Event date: 2024.9.20 - 2024.9.22

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • Identification of nudix hydrolase that eliminates oxidized nucleotide in tomato

    Sotaro Yokoyama, Kazuhide Rikiishi, Manabu Sugimoto

    2024.3.26 

     More details

    Event date: 2024.3.24 - 2024.3.27

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Effect of anthocyanin on viability of rice seeds in space

    Sugimoto, M, Mita, H, Yokobori, S

    7th Global Moon Village Workshop & Symposium  2023.12.7 

     More details

    Event date: 2023.12.6 - 2023.12.10

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • RNA-seq analysis of developing seeds in a wheat mutant RSD32 with reduced seed dormancy

    Rikiishi, K, Sugimoto, M, Maekawa, M

    15th International Symposium on Pre-Harvest Sprouting in Cereals  2023.10.5 

     More details

    Event date: 2023.10.4 - 2023.10.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 酸化ヌクレオチド消去をするイネOsNUDX2の転写エラー抑制機能

    杉本 学, 力石和英, 近藤雄基

    日本宇宙生物科学会第37回大会  2023.9.23 

     More details

    Event date: 2023.9.22 - 2023.9.24

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Transformation of major ginsenosides into minor ginsenosides in ginseng by pickling in salted rice malt pasteM

    Manabu Sugimoto, Nobutada Murakami

    22nd International Congress of Nutrition  2022.12.9 

     More details

    Event date: 2022.12.6 - 2022.12.11

    Language:English   Presentation type:Poster presentation  

    researchmap

  • イネアルビノ様葉緑素変異体 pyl-stb の幼苗致死性に関する光応答制御機構の解析

    力石 和英, 小野 蒼生, 前川 雅彦, 杉本 学

    日本育種学会第142回講演会  2022.9.24 

     More details

    Event date: 2022.9.23 - 2022.9.25

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 酸化ヌクレオチド消去に関与するイネNudix hydrolaseの探索

    近藤雄基,力石和英,杉本 学

    日本宇宙生物科学会第36回大会  2022.9.17 

     More details

    Event date: 2022.9.16 - 2022.9.18

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Change to super early-ripening barley and expression of flowering-control genes by disturbance of lighting condition

    Manabu Sugimoto

    44th Scientific Assembly of the Committee on Space Research  2022.7.17 

     More details

    Event date: 2022.7.16 - 2022.7.24

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 光環境撹乱による作物の極早生化と花成制御関連遺伝子の発現

    杉本 学

    日本宇宙生物科学会第35回大会  2021.9.24 

     More details

    Event date: 2021.9.24 - 2021.9.26

    Presentation type:Oral presentation (general)  

    researchmap

  • 麹床を用いた高麗人参含有メジャージンセノサイドのマイナージンセノサイドへの変換

    杉本 学, 村上允唯

    日本農芸化学会2021年度大会  2021 

     More details

    Event date: 2021.3.18 - 2021.3.21

    Presentation type:Oral presentation (general)  

    researchmap

  • 葉緑体膜の形成と機能維持に重要なVIPP1が示す新奇ATPaseおよびGTPase活性の異なる特徴

    大西紀和, 杉本 学, 張林剛, 坂本 亘

    第62回日本植物生理学会年会  2021 

     More details

    Event date: 2021.3.14 - 2021.3.16

    Presentation type:Oral presentation (general)  

    researchmap

  • Brachypodium nudix hydrolase gene induced by UV irradiation

    Sugimoto, M, Tanaka, M, Iamshchikov, I, Kato, Y, Sakamoto, W, Gusev, O, Sabirov, R

    43rd Scientific Assembly of the Committee on Space Research  2021 

     More details

    Event date: 2021.1.28 - 2021.2.4

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • ポストたんぽぽ計画の進捗状況:主として曝露実験について

    三田肇, 矢野創, 左近樹, 小林憲正, 癸生川陽子, 横谷香織, 中川和道, 杉本学, Tetyana Milojevic, 山岸明彦, 別所義隆

    第21回宇宙科学シンポジウム  2021.1.6 

     More details

    Event date: 2021.1.6 - 2021.1.7

    Presentation type:Oral presentation (general)  

    researchmap

  • 地球生物の宇宙生存可能性検証のための短期宇宙曝露実証実験システムの構築

    横堀伸一, 鳴海一成, 時下進一, 志賀靖弘, 杉本 学, 三田 肇, 橋本博文

    第34回宇宙環境利用シンポジウム  2020.1 

     More details

    Event date: 2020.1.21 - 2020.1.22

    Presentation type:Oral presentation (general)  

    researchmap

  • 地球生物の宇宙生存可能性検証のための短期宇宙曝露実験システムの構築

    横堀伸一, 時下進一, 志賀靖弘, 鳴海一成, 杉本 学, 今井栄一, 三田 肇

    極限環境生物学会2019年度(第20回)年会  2019 

     More details

    Event date: 2019.11.16 - 2019.11.17

    Presentation type:Oral presentation (general)  

    researchmap

  • 異なる大気環境下で太陽光に曝露した大麦種子の農業形質

    杉本 学, 石井 誠, Gusev, O, Sychev, V, Levinskikh, M, ovikova, N, Grigoriev, A

    日本宇宙生物科学会第33回大会  2019.9.21 

     More details

    Event date: 2019.9.21 - 2019.9.22

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 宇宙環境における植物の生存能力と遺伝子発現への影響 Invited

    杉本 学

    日本植物学会第83回大会  2019.9.16 

     More details

    Event date: 2019.9.15 - 2019.9.17

    Language:Japanese  

    researchmap

  • 宇宙空間環境下と火星地表環境下で太陽光に曝露した大麦種子の生存能力

    杉本 学, 石井 誠, Gusev, O, Sychev,V.、Levinskikh,M, Novikova, N, Grigoriev, A

    日本宇宙生物科学会第32回大会  2018.9.22 

     More details

    Event date: 2018.9.22 - 2018.9.23

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Viability and Biological Properties of Barley Seeds under Space and Mars Environments

    Sugimoto, M, Ishii, M, Gusev, O, Sychev, V, Levinskikh, M, Novikova, N, Grigoriev, A

    42th Scientific Assembly of the Committee on Space Researc  2018 

     More details

    Event date: 2018.7.14 - 2018.7.22

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 紫外線で誘導されるミナトカモジグサ由来NUDX遺伝子の機能解析

    日本宇宙生物科学会大会第31回大会  2017 

     More details

  • オオムギ由来L-メチオニンγ-リアーゼの構造と機能解析

    第40回日本分子生物科学会大会  2017 

     More details

  • Transcriptome Analysis of Rice Seeds after Long-term Exposure to Outside of International Space Station.

    11th Asian Microgravity Symposium  2016 

     More details

  • Transcriptome Analysis of Rice Seeds after Long-term Exposure to Outside of International Space Station.

    11th Asian Microgravity Symposium  2016 

     More details

  • 国際宇宙ステーション船外に長期間曝露したイネ種子の生存能力とトランスクリプトーム解析

    日本宇宙生物科学会大会第30回大会  2016 

     More details

  • 紫外線で誘導されるミナトカモジグサ由来Nudix hydrolase遺伝子

    日本宇宙生物科学会大会第29回大会  2015 

     More details

  • Resutls of studies on long-term exposition of dormant forms of various organisms in outer space environment.

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Tanaka, S., Kihara, M., and Sugimoto, M. Expression of nudix hydrolase genes in barley under UV irradiation

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Low GI food with barley in space foods.

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Resutls of studies on long-term exposition of dormant forms of various organisms in outer space environment.

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Tanaka, S., Kihara, M., and Sugimoto, M. Expression of nudix hydrolase genes in barley under UV irradiation

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Low GI food with barley in space foods.

    40th Scientific Assembly of the Committee on Space Research  2014 

     More details

  • Surviving rules: wide overlap in gene expression response to desiccation and ionizing radiation in an anhydrobiotic midge

    12th European Workshop on Astrobiology  2012 

     More details

  • Utilization of barley for space foods

    Bioactive Okayama 2012  2012 

     More details

  • 宇宙環境で生育するミズナの遺伝子発現解析

    日本農芸化学会中四国支部第32回講演会  2012 

     More details

  • Gene expression profile of Mizuna (Brassica rapa) grown under a space environment

    39th COSPAR Scientific Assembly  2012 

     More details

  • Field performance and grain content of ‘Space Barley’, grain of malting barley stored outside international space station for thirteen months

    39th COSPAR Scientific Assembly  2012 

     More details

  • Survival in extreme environment by “preserve-expand-specialize” strategy: lessons from comparative genomics of an anhydrobiotic midge

    39th COSPAR Scientific Assembly  2012 

     More details

  • Role of the barley in space foods

    39th COSPAR Scientific Assembly  2012 

     More details

  • Utilization of barley for space foods

    Bioactive Okayama 2012  2012 

     More details

  • 宇宙環境で生育するミズナの細胞壁代謝関連酵素遺伝子の発現

    日本宇宙生物科学会第26回大会  2012 

     More details

  • 国際宇宙ステーション船外に長期間曝露した大麦種子の生存能力

    日本宇宙生物科学会第26回大会  2012 

     More details

  • 血糖値の上がりにくい宇宙食に関する研究 -麦30%混合玄米の官能試験並びに血糖値測定結果-

    日本宇宙生物科学会第25回大会  2011 

     More details

  • Taxonomic status of Rossia palpebrosa Owen, 1834 and R> glaucopie Loven, 1846 (Cephalopoda: Sepiolida) on molecular-genetic data

    EuroCeph 2011  2011 

     More details

  • Effect of long-term outer space flight on barley seeds

    11th European Workshop on Astrobiology  2011 

     More details

  • 国際宇宙ステーション船外に曝露した大麦種子の生存能力

    日本宇宙生物科学会第25回大会  2011 

     More details

  • 宇宙環境で生育するミズナのストレス応答・防御遺伝子の発現

    日本宇宙生物科学会第25回大会  2011 

     More details

  • The sleeping chionomid: an insect survived 18 months of exposure to outer space

    38th COSPAR Scientific Assembly  2010 

     More details

  • Viability and Biological Properties of Barley Seeds Expose to Outside of International Space Station

    38th COSPAR Scientific Assembly  2010 

     More details

  • Brewing Performance of 'Space Barley', Grain of Malting Barley Exposed to Space

    38th COSPAR Scientific Assembly  2010 

     More details

  • Results of The first stage (2002-2009) of investigation of higher plants onboard RS ISS, as an element of future closed Life Support Systems

    38th COSPAR Scientific Assembly  2010 

     More details

  • Growth and gene expression of barley exposed to space.

    第26回資源生物科学国際シンポジウム  2009 

     More details

  • Space environment in international space station is not stressful for barley

    17th IAA Humans in Space Symposium  2009 

     More details

  • 宇宙大麦の特性解析II: 宇宙環境に曝露した大麦種子の後代における食品安全性試験

    日本農芸化学会2009年度大会  2009 

     More details

  • Space environment in international space station is not stressful for barley

    17th IAA Humans in Space Symposium  2009 

     More details

  • 宇宙大麦の特性解析III: 宇宙環境に曝露した大麦種子の後代より醸造されたビール「Space Barley」の特性評価

    日本育種学会第116回講演会  2009 

     More details

  • Oxidative Stress to Barley Grown in International Space Station

    日本宇宙生物科学会第23回大会  2009 

     More details

  • ACC酸化酵素遺伝子を恒常的に発現する塩ストレス抵抗性大麦におけるストレス応答・防御関連遺伝子の発現

    第31回日本分子生物学会年会・第81回日本生化学会合同大会  2008 

     More details

  • 宇宙環境で生育する大麦の遺伝子発現

    第24回宇宙利用シンポジウム  2008 

     More details

  • 宇宙教育実験「宇宙カイコ」

    第24回宇宙利用シンポジウム  2008 

     More details

  • Proteomics Approach for Identification of Specific Proteins in Salt Tolerant Barley

    10th International Barley Genetics Symposium  2008 

     More details

  • ミミズ由来のセリンプロテアーゼのリパーゼ反応について

    日本農芸化学会中四国支部第21回講演会  2008 

     More details

  • Expression of stress/defense-related genes in barley grown under space environment

    37th COSPAR Scientific Assembly  2008 

     More details

  • 宇宙環境における大麦の生育とストレス

    日本宇宙生物科学会第22回大会  2008 

     More details

  • 宇宙環境で生育する大麦のストレス応答・防御遺伝子の発現

    日本育種学会第114回講演会  2008 

     More details

  • 宇宙大麦の特性解析I-宇宙環境に曝露した大麦種子の後代における農業特性調査-

    日本育種学会第114回講演会  2008 

     More details

  • シロイヌナズナ由来セリンラセマーゼの局在と発現

    第30回日本分子生物学会年会  2007 

     More details

  • オオムギ由来セリンラセマーゼ遺伝子の構造と機能解析

    日本農芸化学会2007年度大会  2007 

     More details

  • オオムギ由来D-システインデスルフヒドラーゼ遺伝子の構造と機能解析

    日本農芸化学会2007年度大会  2007 

     More details

  • 耐乾性小麦の生育過程における細胞壁多糖の変化

    日本農芸化学会2007年度大会  2007 

     More details

  • オオムギ由来セリンラセマーゼの構造と機能解析

    日本ビタミン学会第59回大会  2007 

     More details

  • 植物由来セリンラセマーゼの構造と機能解析

    2007年度酵素・補酵素研究会  2007 

     More details

  • 植物のD-アミノ酸代謝酵素 -セリンラセマーゼ-

    日本農芸化学会中四国支部創立5周年記念若手研究者交流シンポジウム  2006 

     More details

  • 塩ストレス抵抗性オオムギで特異的に発現する遺伝子の解明

    科学技術振興機構「植物の機能と制御」第4回公開シンポジウム  2006 

     More details

  • 醸造オオムギの幼芽における網羅的蛋白質地図の作成

    科学技術振興機構「植物の機能と制御」第4回公開シンポジウム  2006 

     More details

  • 水環境とオオムギの遺伝子発現制御

    第47回日本植物生理学会年会  2006 

     More details

  • シロイヌナズナ由来セリンラセマーゼ遺伝子の構造と機能解析

    日本農芸化学会2006年度大会  2006 

     More details

  • シロイヌナズナ由来セリンラセマーゼ遺伝子の発現

    日本農芸化学会中四国支部創立5周年記念第15回講演会  2006 

     More details

  • シロイヌナズナ由来セリンラセマーゼ遺伝子の機能と発現

    日本ビタミン学会第58回大会  2006 

     More details

  • 植物のD-アミノ酸代謝酵素の構造と機能

    自然科学研究機構基礎生物学研究所部門公開セミナー  2006 

     More details

  • Serine racemase from Arabidopsis thaliana is a bifunctional enzyme catalyzing recemization of serine and dehydration of serine to pyruvate

    20th IUBMU International Congress of Biochemistry and Molecular Biology  2006 

     More details

  • Earthworm serine protease: Characterization, molecular cloning, and application of the catarytic functions.

    International Chemical Congress of Pacific Basin Societies 2005  2005 

     More details

  • 塩ストレス抵抗性オオムギで発現するACC酸化酵素遺伝子のクローニング

    日本農芸化学会中四国支部第11回講演会  2005 

     More details

  • 塩ストレス抵抗性オオムギに関する研究3.恒常的に高発現するACC酸化酵素遺伝子の機能.

    日本育種学会第107・108回講演会  2005 

     More details

  • 塩抵抗性オオムギ根で特異的に発現しているタンパク質のプロテオーム解析.

    日本育種学会第107・108回講演会  2005 

     More details

  • ストレス応答遺伝子の解析 -塩ストレス抵抗性植物作出に向けて-

    2005年度岡山大学重点プロジェクト「植物医科学」第2回シンポジウム  2005 

     More details

  • オオムギの網羅的タンパク質マップ構築のためのプロテオーム解析

    第28回日本分子生物学会年会  2005 

     More details

  • Molecular cloning and expression of cDNA encoding serine protease with high fibrinolytic activity from earthworm

    第77回日本生化学会大会  2004 

     More details

  • Root-specific O-methyltransferase gene expressed in salt-tolerant barley

    9th International barley genetics symposium  2004 

     More details

  • ストレスに打ち勝つ農作物の創製:環境遺伝子化学的アプローチ

    岡山県立大学サテライトキャンパス「9月の大学院講座」  2004 

     More details

  • オオムギの網羅的タンパク質マップ構築の試み

    日本育種学会第106回講演会  2004 

     More details

  • 塩ストレス条件下における塩抵抗性オオムギ根cDNAのEXPRESSED SEQUENCE TAG(EST)解析

    第26回日本分子生物学会年会  2003 

     More details

  • Penicillium chrysogenum IFO4626から誘導したアルミニウム耐性変異株で優位に発現する遺伝子の構造と機能解析

    日本農芸化学会中四国支部例会第5回講演会  2003 

     More details

  • ミミズプロテアーゼの精製と性質、およびその応用

    第一回貧毛類分子生物研究会  2003 

     More details

  • ミミズ由来プロテアーゼの構造と機能解析

    第一回貧毛類分子生物研究会  2003 

     More details

  • 塩ストレス条件下における塩抵抗性オオムギ根cDNAのEST解析による特異的発現遺伝子の解明

    CREST「オオムギゲノム機能の開発と制御」成果発表国際シンポジウム  2003 

     More details

  • 塩ストレス条件下における塩抵抗性オオムギ根cDNAのEST解析による特異的発現遺伝子の解明

    科学技術振興事業団「植物の機能と制御」第1回公開シンポジウム  2003 

     More details

  • Expressed sequence tag (EST) analysis of the root cDNA library from salt-tolerant barley under salt stress

    Plant & Animal Genome XI  2003 

     More details

  • Penicillium chrysogenum IFO4626よりUV照射で誘導されたアルミニウム耐性変異株で特異的に発現する遺伝子の解析

    第25回日本分子生物学会年会  2002 

     More details

  • 塩ストレス抵抗性オオムギに関する研究.1.塩ストレスで特異的に発現する遺伝子の解析

    日本育種学会第101回講演会  2002 

     More details

  • ストレス抵抗性オオムギに関する研究.2.塩ストレスで誘導されるO-methyltransferase遺伝子のクローニングと構造

    日本育種学会第102回講演会  2002 

     More details

  • 環形動物由来の新規なセリンプロテアーゼ:その触媒機能の多目的応用(その2)

    第5回生体触媒化学シンポジウム  2001 

     More details

  • Identification of specific genes expressed in salt tolerant barley

    Plant & Animal Genome IX  2001 

     More details

  • 糸状菌Mucor javanicus由来α-グルコシダーゼの基質特異性に関与する構造解析

    日本農芸化学会2001年度大会  2001 

     More details

  • ホップのファルネシンピロリン酸シンターゼ遺伝子のクローニングと解析

    日本育種学会第99回講演会  2001 

     More details

  • ポリアルキレングリコール分解微生物の分離

    日本農芸化学会2000年度大会  2000 

     More details

  • Cloning and expression of the polyethylene glycol dehydrogenase gene from Sphingomonas terrae

    American Society for Mocrobiology The 100th General meeting  2000 

     More details

  • Crude oil-emulsifying compounds produced by Alcanivorax sp. ST-T1

    American Society for Mocrobiology The 100th General meeting  2000 

     More details

  • Sphingomonas terrae由来ポリエチレングリコール脱水素酵素遺伝子のクローニングと構造解析

    日本農芸化学会2000年度大会  2000 

     More details

  • 大腸菌で発現するSphingomonas terrae由来ポリエチレングリコール脱水素酵素遺伝子の精製と性質

    日本農芸化学会2000年度大会  2000 

     More details

  • Alcanivorax sp. ST-T1による原油乳化性バイオサーファクタントの生産と特性

    日本農芸化学会2000年度大会  2000 

     More details

  • Isolation and characterization of acid and Al-tolerant Microorganisms

    日本農芸化学会2000年度大会  2000 

     More details

  • Isolation and characterization of aluminium-tolerant microorganisms

    日本生物工学会大会  1999 

     More details

  • 植物由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと構造解析

    日本農芸化学界1999年度大会  1999 

     More details

  • 合成高分子代謝における細菌細胞外膜機能

    日本農芸化学会1999年度大会  1999 

     More details

  • Structure and function of plant genes induced by oxidative stress

    Agricultural Science and Technology for the Next Generation  1999 

     More details

▼display all

Industrial property rights

  • 高麗人参の発酵物の製造法法

    杉本 学, 池田 昭

     More details

    Application no:特願2022-029658  Date applied:2020.2.25

    Patent/Registration no:特許7020752  Date registered:2022.2.8 

    researchmap

Awards

  • 日本農芸化学会中四国支部奨励賞

    2004  

     More details

    Country:Japan

    researchmap

Research Projects

  • 光環境撹乱による作物の極早生化機構の解明とスマートアグリへの応用

    Grant number:20K06343  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    杉本 学

      More details

    Grant amount:\3900000 ( Direct expense: \3000000 、 Indirect expense:\900000 )

    本研究では、赤色光や遠赤色光補光連続照射により起こる大麦花成促進遺伝子FT1発現制御機構を明らかにし早生化や極早生化が起こる分子メカニズムを明らかにするための研究を進める。
    昨年度の研究により遠赤色光補光連続照射でFT1遺伝子の発現ピークより約5日前に発現ピークとなるPpd-H1遺伝子について、本年度研究では、蛍光灯+赤色光補光連続照射で早生化し蛍光灯+遠赤色光補光連続照射で極早生化する大麦のうち産地が異なる3品種のPpd-H1遺伝子発現量の経時的変化をリアルタイムPCR法により解析したところ、2品種が遠赤色光補光連続照射でPpd-H1遺伝子の発現ピークがFT1遺伝子の発現ピークより約5日前に出現することを確認した。そこで、Ppd-H1遺伝子プロモーター領域と考えられる開始コドン上流350bp塩基をクローニングした。プロモーター領域をバイナリーベクターにあるGUS遺伝子上流に挿入したプラスミドを作製し、形質転換体(T0)をスクリーニングしている。

    researchmap

  • Development of recycling biomass for recycling systems of organic waste

    Grant number:15580297  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SUGIMOTO Manabu

      More details

    Grant amount:\3200000 ( Direct expense: \3200000 )

    The genes encoding pro-form and active-form of strong fibrinolytic protease, isozme A, in the six isozymes of eartworm, Lumbricus rubellus, were subcloned into a plasmid, pEU3-NII, and expressed in the wheat germ cell-free protein synthesis system. Extra proteins with a molecular mass of about 26 and 25 kDa were produced by pro-form and active-form of plasmids, respectively, which are similar to calculated molecular mass from the amino acid sequences. These results shows that the gene from earthworm could express in plant.
    The pro-form and active-form genes of isozyme A were subcloned into a plasmid pBI121, resulting the plasmids both of which the enzyme genes fused with CaMV35S promoter. These genes were transferred to Arabidopsis thaliana, cv. Columbia, and T1 seeds were obtained. T1 seeds including active-form gene were cultured on MS medium agar plate containing kanamycine and three types of plants, 1) normal shape, 2) breached leaf, 3) wrinkled leaf, were observed. All types of transgenic plants expressed extra isozyme A mRNA, but could not produce T2 seeds. The transgenic plants of T1 seeds including pro-form gene also showed the wrinkled leaves which is breached gradually, resulting the die. These results suggest that the isozyme A from earthworm is produced and works in plants, and the possibility of producing the organic waste-degrading plants if the expression of the isozyme A gene could be controlled by adequate promoters which can restrict the expression strongly or organ-specific expression affecting little on plant growth.

    researchmap

  • Function of the plant gene induced by oxidative stress on the reduciton of lipid hydroperoxide

    Grant number:12660295  2000 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SUGIMOTO Manabu

      More details

    Grant amount:\3100000 ( Direct expense: \3100000 )

    The gene that includes the promoter region of the phospholipid hydroperoxide glutathione peroxidase (PHGPX)-like protein was cloned from Arabidopsis thaliana. The gene was subcloned into a plasmid, pBI101, resulting the promoter gene was fused with the β-glucuronidase (GUS) gene. The fused gene was transferred to the root of the pea by the particle bombardment system. Transient expression of the GUS was shown in the roots cultured by the MS medium containing 0, 50, and 100mM NaCl. This result suggests that the PHGPX-like gene is not induced by oxidative/salt stress.
    A cDNA encoding barley PHGPX-like protein was expressed in Escherichia coli cells to produce an extra protein. The PHGPX was not detected in the cell-free extract prepared from the clone cells when tert-butyl hydroperoxide, cumene hydroperoxide, and hydrogen peroxide were used as a substrate. The clone cells were assayed for their ability to grow in the medium containing NaCl. The clone cells were much more tolerant to NaCl stress than the host cells, suggesting that the PHGPX-like protein would be a good candidate component of anti-salt/oxidative enzyme in plants.

    researchmap

  • Studies of Earthworm Protease for Application of the Catalytic Function

    Grant number:10556023  1998 - 2000

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B).

    NAKAJIMA Nobuyoshi, NAKAMURA Kaoru, ISHIHARA Kohji, SUGIMOTO Manabu, HAMADA Hiroki

      More details

    Grant amount:\7700000 ( Direct expense: \7700000 )

    Earthworm serine proteases were investigated for application of the catalytic function and the many useful results were obtained as bellows.
    1. Purification and characterization of the proteases.
    2. Chemical modification of the protease as a therapeutic enzyme.
    3. Construction of an immobilized enzyme bio-reactor.
    4. Analysis and utilization of the earthworm autolysate
    5. Gene cloning and expression of the proteases.
    6. Investigation of structure, function, and stability of the proteases.
    7. Investigation of DNA and amino acid sequences of the proteases.
    8. Large-scale production of the recombinant protease from Yeast cells.

    researchmap

  • Enzymatic Glycosylation and Phosphatidylation in Organic Solvent Systems.

    Grant number:05660095  1993 - 1994

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for General Scientific Research (C)

    SUZUKI Yukio, SUGIMOTO Manabu

      More details

    Grant amount:\2100000 ( Direct expense: \2100000 )

    1.Enzymatic glycosylation of fat-solubly physiologically active compounds in a hydrophilic organic solvent.
    New derivatives of long acyclic terpene alcohols, O-beta-glucosylfarnesol, O-beta-galactosylfarnesol and O-beta-galactosylgeranylgeraniol, were found to be formed, when the mixture containing these terpene alcohols and cellobiose (or lactose) in 50 % acetonitrile was incubated with almond beta-glucosidase (or Aspergillus oryzae beta-galactosidase). These glycosylated derivatives were isolated and identified.
    Glucosylation of phenolic compounds-A high transglucosylation reaction to hydroxy residue of aromatic compounds.
    The synthesis of pheny1-alpha-glucoside and 0-alpha-glucosylcatechol via transglucosylation reaction by Bacillus stearothermophilus cyclomaltodextrin glucanotransferase was found. These glucosides were isolated in crystalline form and identified. These glucosides were much more stable than the aglycones in a peroxidase-hydrogen peroxide-oxidizing system.
    3.Enzymatic phosphatidylation of water-soluble vitamins in organic solvents.
    Streptomyces sp. phospholipase D was found to be catalyze the transfer reaction of the dipalmitoylphosphatidyl residue from 1,2-dipalmitoy1-3-sn-phosphatidy1-choline (DPPC) to water-soluble vitamins (thiamin, pantothenic acid, and these related compounds) in a biphasic system, to afford novel fat-soluble derivatives of the vitamins (DPP-thiamin, DPP-pantothenic acid, DPP-thiamin-propyldisulfide, DPP-fursulthiamin, and DPP-pantothenyl ethyl ether), respectively. These novel derivatives were isolated and identified.

    researchmap

 

Class subject in charge

  • Plant Cellular Biochemistry (2025academic year) Prophase  - その他

  • Plant Cytomolecular Biochemistry (2025academic year) Late  - 水5~8

  • Seminar in Plant Cytomolecular Biochemistry (2025academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2025academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2025academic year) Other  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2025academic year) Year-round  - その他

  • Advanced Study (2025academic year) Other  - その他

  • Plant Cellular Biochemistry (2024academic year) Prophase  - その他

  • Plant Cytomolecular Biochemistry (2024academic year) Late  - 水5~8

  • Seminar in Plant Cytomolecular Biochemistry (2024academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2024academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2024academic year) Year-round  - その他

  • Advanced Study (2024academic year) Other  - その他

  • Plant Nutrition (2023academic year) Fourth semester  - 水1,水2

  • Plant Cellular Biochemistry (2023academic year) Prophase  - その他

  • Plant Cellular Biochemistry (2023academic year) Prophase  - その他

  • Plant Cytomolecular Biochemistry (2023academic year) Late  - 水5~8

  • Plant Cytomolecular Biochemistry (2023academic year) Late  - 水5~8

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2023academic year) Year-round  - その他

  • Advanced Study (2023academic year) Other  - その他

  • Specific Research of Bioresources Science (2023academic year) Year-round  - その他

  • Plant Cellular Biochemistry (2022academic year) Prophase  - その他

  • Plant Cytomolecular Biochemistry (2022academic year) Late  - 水5~8

  • Seminar in Plant Cytomolecular Biochemistry (2022academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2022academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2022academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2022academic year) Prophase  - その他

  • Specific Research of Bioresources Science (2022academic year) Year-round  - その他

  • Plant Cellular Biochemistry (2021academic year) Prophase  - その他

  • Plant Cytomolecular Biochemistry (2021academic year) Late  - 水5~8

  • Seminar in Plant Cytomolecular Biochemistry (2021academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2021academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2021academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2021academic year) Prophase  - その他

  • Specific Research of Bioresources Science (2021academic year) Year-round  - その他

  • Plant Nutrition (2020academic year) Fourth semester  - 水1,水2

  • Plant Cellular Biochemistry (2020academic year) Prophase  - その他

  • Plant Cytomolecular Biochemistry (2020academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2020academic year) Prophase  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2020academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2020academic year) Late  - その他

  • Seminar in Plant Cytomolecular Biochemistry (2020academic year) Prophase  - その他

  • Specific Research of Bioresources Science (2020academic year) Year-round  - その他

▼display all

 

Social Activities

  • 植物が切り開く宇宙生活

    Role(s):Lecturer

    浅口市教育委員会 岡山天文博物館  宇宙☆自然講座  2022.9.4

     More details

    Type:Lecture

    researchmap

Media Coverage

  • 放射線が飛び交う宇宙でも紫米なら保存可能! Newspaper, magazine

    子供の科学  2025.4

     More details

    Author:Other 

    researchmap

  • 宇宙保存の紫米 生育率3倍超に 岡山大確認 白米より遺伝損傷軽く Newspaper, magazine

    山陽新聞  2025.2.17

     More details

    Author:Other 

    researchmap

  • 紫米は宇宙に強い 色素が放射線から守る 岡山大など研究グループ Newspaper, magazine

    日本農業新聞  2025.2.7

     More details

  • 宇宙の大麦大きく育て 児童ら種まき Newspaper, magazine

    上毛新聞  2019.12

     More details

  • 「宇宙を旅した大麦」収穫 Newspaper, magazine

    上毛新聞  2019.6

     More details

  • 宇宙帰りの大麦収穫 Newspaper, magazine

    上毛新聞  2019.5

     More details

▼display all