Updated on 2022/09/26

写真a

 
MIYAZAKI Ikuko
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Lecturer
Position
Lecturer
External link

Degree

  • Ph.D. ( Okayama University )

Research Interests

  • Neurodegenerative disease

  • Astrocyte

  • Parkinson's disease

  • Neurochemistry and Neuropharmacology

Research Areas

  • Life Science / Neurology

  • Life Science / Neuroscience-general

Education

  • Okayama University   薬学部   製薬化学科

    1993 - 1997

      More details

    Country: Japan

    researchmap

Research History

  • Senior Assistant Professor, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

    2020.9

      More details

  • Assistant Professor, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

    2007 - 2020.8

      More details

  • 岡山大学大学院医歯薬学総合研究科 助手

    2001 - 2007

      More details

  • Okayama University   Medical School

    2000 - 2001

      More details

Professional Memberships

▼display all

Committee Memberships

  • 日本パーキンソン病・運動障害疾患学会(MDSJ)   評議員  

    2020.7   

      More details

  • 日本神経精神薬理学会   広報委員  

    2016.11   

      More details

    Committee type:Academic society

    researchmap

  • 日本神経精神薬理学会   評議員  

    2015   

      More details

    Committee type:Academic society

    日本神経精神薬理学会

    researchmap

  • 日本薬理学会   評議員  

    2007   

      More details

    Committee type:Academic society

    日本薬理学会

    researchmap

 

Papers

  • Neuron-Astrocyte Interactions in Parkinson’s Disease Invited Reviewed

    Ikuko Miyazaki, Masato Asanuma

    Cells   9 ( 12 )   2623 - 2623   2020.12

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Parkinson’s disease (PD) is the second most common neurodegenerative disease. PD patients exhibit motor symptoms such as akinesia/bradykinesia, tremor, rigidity, and postural instability due to a loss of nigrostriatal dopaminergic neurons. Although the pathogenesis in sporadic PD remains unknown, there is a consensus on the involvement of non-neuronal cells in the progression of PD pathology. Astrocytes are the most numerous glial cells in the central nervous system. Normally, astrocytes protect neurons by releasing neurotrophic factors, producing antioxidants, and disposing of neuronal waste products. However, in pathological situations, astrocytes are known to produce inflammatory cytokines. In addition, various studies have reported that astrocyte dysfunction also leads to neurodegeneration in PD. In this article, we summarize the interaction of astrocytes and dopaminergic neurons, review the pathogenic role of astrocytes in PD, and discuss therapeutic strategies for the prevention of dopaminergic neurodegeneration. This review highlights neuron-astrocyte interaction as a target for the development of disease-modifying drugs for PD in the future.

    DOI: 10.3390/cells9122623

    researchmap

  • Mirtazapine exerts astrocyte-mediated dopaminergic neuroprotection Reviewed

    Ryo Kikuoka, Ikuko Miyazaki, Natsuki Kubota, Megumi Maeda, Daiki Kagawa, Masaaki Moriyama, Asuka Sato, Shinki Murakami, Yoshihisa Kitamura, Toshiaki Sendo, Masato Asanuma

    Scientific Reports   10 ( 1 )   20698 - 20698   2020.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>Mirtazapine, a noradrenergic and specific serotonergic antidepressant (NaSSA), is known to activate serotonin (5-HT) 1A receptor. Our recent study demonstrated that stimulation of astrocytic 5-HT1A receptors promoted astrocyte proliferation and upregulated antioxidative property in astrocytes to protect dopaminergic neurons against oxidative stress. Here, we evaluated the neuroprotective effects of mirtazapine against dopaminergic neurodegeneration in models of Parkinson’s disease (PD). Mirtazapine administration attenuated the loss of dopaminergic neurons in the substantia nigra and increased the expression of the antioxidative molecule metallothionein (MT) in the striatal astrocytes of 6-hydroxydopamine (6-OHDA)-injected parkinsonian mice via 5-HT1A receptors. Mirtazapine protected dopaminergic neurons against 6-OHDA-induced neurotoxicity in mesencephalic neuron and striatal astrocyte cocultures, but not in enriched neuronal cultures. Mirtazapine-treated neuron-conditioned medium (Mir-NCM) induced astrocyte proliferation and upregulated MT expression via 5-HT1A receptors on astrocytes. Furthermore, treatment with medium from Mir-NCM-treated astrocytes protected dopaminergic neurons against 6-OHDA neurotoxicity, and these effects were attenuated by treatment with a MT-1/2-specific antibody or 5-HT1A antagonist. Our study suggests that mirtazapine could be an effective disease-modifying drug for PD and highlights that astrocytic 5-HT1A receptors may be a novel target for the treatment of PD.

    DOI: 10.1038/s41598-020-77652-4

    PubMed

    researchmap

    Other Link: http://www.nature.com/articles/s41598-020-77652-4

  • The Rotenone Models Reproducing Central and Peripheral Features of Parkinson’s Disease Invited Reviewed

    Ikuko Miyazaki, Masato Asanuma

    NeuroSci   1 ( 1 )   1 - 14   2020.8

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Parkinson’s disease (PD) is a complex, multi-system, neurodegenerative disorder; PD patients exhibit motor symptoms (such as akinesia/bradykinesia, tremor, rigidity, and postural instability) due to a loss of nigrostriatal dopaminergic neurons, and non-motor symptoms such as hyposmia, autonomic disturbance, depression, and REM sleep behavior disorder (RBD), which precedes motor symptoms. Pathologically, α-synuclein deposition is observed in the central and peripheral nervous system of sporadic PD patients. To clarify the mechanism of neurodegeneration in PD and to develop treatment to slow or stop PD progression, there is a great need for experimental models which reproduce neurological features of PD. Animal models exposed to rotenone, a commonly used pesticide, have received most attention since Greenamyre and his colleagues reported that chronic exposure to rotenone could reproduce the anatomical, neurochemical, behavioral, and neuropathological features of PD. In addition, recent studies demonstrated that rotenone induced neuropathological change not only in the central nervous system but also in the peripheral nervous system in animals. In this article, we review rotenone models especially focused on reproducibility of central and peripheral multiple features of PD. This review also highlights utility of rotenone models for investigation of PD pathogenesis and development of disease-modifying drugs for PD in future.

    DOI: 10.3390/neurosci1010001

    researchmap

  • Chronic Systemic Exposure to Low-Dose Rotenone Induced Central and Peripheral Neuropathology and Motor Deficits in Mice: Reproducible Animal Model of Parkinson’s Disease Invited Reviewed

    Ikuko Miyazaki, Nami Isooka, Fuminori Imafuku, Jin Sun, Ryo Kikuoka, Chieko Furukawa, Masato Asanuma

    International Journal of Molecular Sciences   21 ( 9 )   3254 - 3254   2020.5

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Epidemiological studies demonstrated that pesticide exposure, such as rotenone and paraquat, increases the risk of Parkinson’s disease (PD). Chronic systemic exposure to rotenone, a mitochondrial complex I inhibitor, could reproduce many features of PD. However, the adoption of the models is limiting because of variability in animal sensitivity and the inability of other investigators to consistently reproduce the PD neuropathology. In addition, most of rotenone models were produced in rats. Here, we tried to establish a high-reproducible rotenone model using C57BL/6J mice. The rotenone mouse model was produced by chronic systemic exposure to a low dose of rotenone (2.5 mg/kg/day) for 4 weeks by subcutaneous implantation of rotenone-filled osmotic mini pump. The rotenone-treated mice exhibited motor deficits assessed by open field, rotarod and cylinder test and gastrointestinal dysfunction. Rotenone treatment decreased the number of dopaminergic neuronal cells in the substantia nigra pars compacta (SNpc) and lesioned nerve terminal in the striatum. In addition, we observed significant reduction of cholinergic neurons in the dorsal motor nucleus of the vagus (DMV) and the intestinal myenteric plexus. Moreover, α-synuclein was accumulated in neuronal soma in the SNpc, DMV and intestinal myenteric plexus in rotenone-treated mice. These data suggest that the low-dose rotenone mouse model could reproduce behavioral and central and peripheral neurodegenerative features of PD and be a useful model for investigation of PD pathogenesis.

    DOI: 10.3390/ijms21093254

    researchmap

  • Effects of maternal bisphenol A diglycidyl ether exposure during gestation and lactation on behavior and brain development of the offspring Reviewed

    Ikuko Miyazaki, Ryo Kikuoka, Nami Isooka, Mika Takeshima, Kanau Sonobe, Rei Arai, Hidemaru Funakoshi, Kyle E. Quin, Jonathan Smart, Kazumasa Zensho, Masato Asanuma

    Food and Chemical Toxicology   138   111235 - 111235   2020.4

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.fct.2020.111235

    researchmap

  • Dopaminergic neuroprotective effects of rotigotine via 5-HT1A receptors: Possibly involvement of metallothionein expression in astrocytes Reviewed

    Nami Isooka, Ikuko Miyazaki, Ryo Kikuoka, Kouichi Wada, Erika Nakayama, Kotaro Shin, Daichi Yamamoto, Yoshihisa Kitamura, Masato Asanuma

    Neurochemistry International   132   104608 - 104608   2020.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.neuint.2019.104608

    PubMed

    researchmap

  • Effects of Enteric Environmental Modification by Coffee Components on Neurodegeneration in Rotenone-Treated Mice Invited Reviewed

    Ikuko Miyazaki, Nami Isooka, Kouichi Wada, Ryo Kikuoka, Yoshihisa Kitamura, Masato Asanuma

    Cells   8 ( 3 )   221 - 221   2019.3

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Epidemiological studies have shown that coffee consumption decreases the risk of Parkinson’s disease (PD). Caffeic acid (CA) and chlorogenic acid (CGA) are coffee components that have antioxidative properties. Rotenone, a mitochondrial complex I inhibitor, has been used to develop parkinsonian models, because the toxin induces PD-like pathology. Here, we examined the neuroprotective effects of CA and CGA against the rotenone-induced degeneration of central dopaminergic and peripheral enteric neurons. Male C57BL/6J mice were chronically administered rotenone (2.5 mg/kg/day), subcutaneously for four weeks. The animals were orally administered CA or CGA daily for 1 week before rotenone exposure and during the four weeks of rotenone treatment. Administrations of CA or CGA prevented rotenone-induced neurodegeneration of both nigral dopaminergic and intestinal enteric neurons. CA and CGA upregulated the antioxidative molecules, metallothionein (MT)-1,2, in striatal astrocytes of rotenone-injected mice. Primary cultured mesencephalic or enteric cells were pretreated with CA or CGA for 24 h, and then further co-treated with a low dose of rotenone (1–5 nM) for 48 h. The neuroprotective effects and MT upregulation induced by CA and CGA in vivo were reproduced in cultured cells. Our data indicated that intake of coffee components, CA and CGA, enhanced the antioxidative properties of glial cells and prevents rotenone-induced neurodegeneration in both the brain and myenteric plexus.

    DOI: 10.3390/cells8030221

    PubMed

    researchmap

  • Region-Specific Neuroprotective Features of Astrocytes against Oxidative Stress Induced by 6-Hydroxydopamine Invited Reviewed

    Masato Asanuma, Nao Okumura-Torigoe, Ikuko Miyazaki, Shinki Murakami, Yoshihisa Kitamura, Toshiaki Sendo

    International Journal of Molecular Sciences   20 ( 3 )   598 - 598   2019.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    In previous studies, we found regional differences in the induction of antioxidative molecules in astrocytes against oxidative stress, postulating that region-specific features of astrocytes lead region-specific vulnerability of neurons. We examined region-specific astrocytic features against dopaminergic neurotoxin 6-hydroxydopamine (6-OHDA) as an oxidative stress using co-culture of mesencephalic neurons and mesencephalic or striatal astrocytes in the present study. The 6-OHDA-induced reduction of mesencephalic dopamine neurons was inhibited by co-culturing with astrocytes. The co-culture of midbrain neurons with striatal astrocytes was more resistant to 6-OHDA than that with mesencephalic astrocytes. Furthermore, glia conditioned medium from 6-OHDA-treated striatal astrocytes showed a greater protective effect on the 6-OHDA-induced neurotoxicity and oxidative stress than that from mesencephalic astrocytes. The cDNA microarray analysis showed that the number of altered genes in both mesencephalic and striatal astrocytes was fewer than that changed in either astrocyte. The 6-OHDA treatment, apparently up-regulated expressions of Nrf2 and some anti-oxidative or Nrf2-regulating phase II, III detoxifying molecules related to glutathione synthesis and export in the striatal astrocytes but not mesencephalic astrocytes. There is a profound regional difference of gene expression in astrocytes induced by 6-OHDA. These results suggest that protective features of astrocytes against oxidative stress are more prominent in striatal astrocytes, possibly by secreting humoral factors in striatal astrocytes.

    DOI: 10.3390/ijms20030598

    PubMed

    researchmap

  • Therapeutic Strategy of Targeting Astrocytes for Neuroprotection in Parkinson's Disease Invited Reviewed

    Ikuko Miyazaki, Masato Asanuma

    Current Pharmaceutical Design   23 ( 33 )   2018.1

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bentham Science Publishers Ltd.  

    DOI: 10.2174/1381612823666170710163731

    Scopus

    researchmap

  • Editorial: Astrocyte-targeted therapeutic strategies for neurological disorders. Invited Reviewed

    Ikuko Miyazaki

    Current Pharmaceutical Design   23 ( 33 )   4933 - 4935   2017

     More details

    Authorship:Lead author, Corresponding author   Language:English  

    researchmap

  • Neuroprotective effects of levetiracetam target xCT in astrocytes in parkinsonian mice Reviewed

    Ikuko Miyazaki, Shinki Murakami, Nao Torigoe, Yoshihisa Kitamura, Masato Asanuma

    JOURNAL OF NEUROCHEMISTRY   136 ( 1 )   194 - 204   2016.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    Astrocytes but not neurons express cystine/glutamate exchange transporter (xCT), which takes up cystine, and consequently supplies the substrate for GSH synthesis in neurons. It is recognized that GSH synthesis in neurons is dependent on the expression of xCT in astrocytes. Previous studies reported that levetiracetam (LEV), an anti-epileptic drug, increased xCT expression invivo. The purpose of this study was to examine neuroprotective effects of LEV in parkinsonian models and demonstrate xCT in astrocytes as a target of neuroprotection against dopaminergic neurodegeneration. We identified striatal astrocytes cultured with LEV showed significant increase in xCT expression and GSH levels. Preincubation of primary cultured mesencephalic dopamine neurons with conditioned media from LEV-treated astrocytes protected against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. These protective effects were canceled by xCT inhibitor. Furthermore, reduction of nigrostriatal dopaminergic neurons in 6-OHDA-lesioned parkinsonian mice was significantly abrogated by repeated injections of LEV. Treatment with LEV significantly increased the expression of xCT in striatal astrocytes in the hemi-parkinsonian mice. In conclusion, LEV exerts neuroprotective effects against neurodegeneration via up-regulation of xCT and GSH in astrocytes. Thus, xCT in astrocytes could be a potential target in novel neuroprotective approaches to prevent degeneration of dopaminergic neurons.

    DOI: 10.1111/jnc.13405

    Web of Science

    researchmap

  • Serotonin 1A Receptors on Astrocytes as a Potential Target for the Treatment of Parkinson's Disease Reviewed

    Ikuko Miyazaki, Masato Asanuma

    CURRENT MEDICINAL CHEMISTRY   23 ( 7 )   686 - 700   2016

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:BENTHAM SCIENCE PUBL LTD  

    Astrocytes are the most abundant neuron-supporting glial cells in the central nervous system. The neuroprotective role of astrocytes has been demonstrated in various neurological disorders such as amyotrophic lateral sclerosis, spinal cord injury, stroke and Parkinson's disease (PD). Astrocyte dysfunction or loss-of-astrocytes increases the susceptibility of neurons to cell death, while astrocyte transplantation in animal studies has therapeutic advantage. We reported recently that stimulation of serotonin 1A (5-HT1A) receptors on astrocytes promoted astrocyte proliferation and upregulated antioxidative molecules to act as a neuroprotectant in parkinsonian mice. PD is a progressive neurodegenerative disease with motor symptoms such as tremor, bradykinesia, rigidity and postural instability, that are based on selective loss of nigrostriatal dopaminergic neurons, and with non-motor symptoms such as orthostatic hypotension and constipation based on peripheral neurodegeneration. Although dopaminergic therapy for managing the motor disability associated with PD is being assessed at present, the main challenge remains the development of neuroprotective or disease-modifying treatments. Therefore, it is desirable to find treatments that can reduce the progression of dopaminergic cell death. In this article, we summarize first the neuroprotective properties of astrocytes targeting certain molecules related to PD. Next, we review neuroprotective effects induced by stimulation of 5-HT1A receptors on astrocytes. The review discusses new promising therapeutic strategies based on neuroprotection against oxidative stress and prevention of dopaminergic neurodegeneration.

    DOI: 10.2174/0929867323666160122115057

    Web of Science

    researchmap

  • Serotonin-1A agonist 8-OH-DPAT alleviates motor dysfunction and motor neuron degeneration in a model of amyotrophic lateral sclerosis. Invited Reviewed

    Ikuko Miyazaki, Shinki Murakami, Takashi Nakano, Nao Torigoe, Ryo Kikuoka, Yoshihisa Kitamura, Toshiaki Sendo, Masato Asanuma

    Annals of Pharmacology and Pharmaceutics   1 ( 1 )   1003 - 1003   2016

     More details

    Authorship:Lead author, Corresponding author   Language:English  

    researchmap

  • Targeting 5-HT1A receptors in astrocytes to protect dopaminergic neurons in parkinsonian models Reviewed

    Ikuko Miyazaki, Masato Asanuma, Shinki Murakami, Mika Takeshima, Nao Torigoe, Yoshihisa Kitamura, Ko Miyoshi

    Neurobiology of Disease   59 ( 59 )   244 - 256   2013.11

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Astrocytes are abundant neuron-supporting glial cells that harbor a powerful arsenal of neuroprotective antioxidative molecules and neurotrophic factors. Here we examined whether enrichment with healthy striatal astrocytes can provide neuroprotection against progressive dopaminergic neurodegeneration. Serotonin 1A (5-HT1A) agonist 8-OH-DPAT induced astrocyte proliferation and increased metallothionein-1/-2 (MT-1/-2), antioxidative molecules, in cultured astrocytes and the striatum of mice. Primary cultured mesencephalic dopamine neurons were protected against oxidative stress by preincubation with conditioned media from 8-OH-DPAT-treated astrocytes. These protective effects were canceled by 5-HT1A antagonist or MT-1/-2-specific antibody. Furthermore, reduction of nigrostriatal dopaminergic neurons in 6-hydroxydopamine-lesioned parkinsonian model mice was significantly abrogated by repeated injections of 8-OH-DPAT. Treatment with 8-OH-DPAT markedly increased the expression of MT in striatal astrocytes in the hemi-parkinsonian mice. Our study provides a promising therapeutic strategy of neuroprotection against oxidative stress and progressive dopaminergic neurodegeneration by demonstrating the efficacy of targeting 5-HT1A receptors in astrocytes. © 2013 Elsevier Inc.

    DOI: 10.1016/j.nbd.2013.08.003

    Scopus

    PubMed

    researchmap

  • Astrocyte-Derived Metallothionein Protects Dopaminergic Neurons from Dopamine Quinone Toxicity Reviewed

    Ikuko Miyazaki, Masato Asanuma, Yuri Kikkawa, Mika Takeshima, Shinki Murakami, Ko Miyoshi, Norio Sogawa, Taizo Kita

    GLIA   59 ( 3 )   435 - 451   2011.3

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-LISS  

    Our previous studies demonstrated the involvement of quinone formation in dopaminergic neuron dysfunction in the L-DOPA-treated parkinsonian model and in methamphetamine (METH) neurotoxicity. We further reported that the cysteine-rich metal-binding metallothionein (MT) family of proteins protects dopaminergic neurons against dopamine (DA) quinone neurotoxicity by its quinone-quenching property. The aim of this study was to examine MT induction in astrocytes in response to excess DA and the potential neuroprotective effects of astrocyte-derived MTs against DA quinone toxicity. DA exposure significantly upregulated MT-1/-2 in cultured striatal astrocytes, but not in mesencephalic neurons. This DA-induced MT upregulation in astrocytes was blocked by treatment with a DA-transporter (DAT) inhibitor, but not by DA-receptor antagonists. Expression of nuclear factor erythroid 2-related factor (Nrf2) and its binding activity to antioxidant response element of MT-1 gene were significantly increased in the astrocytes after DA exposure. Nuclear translocation of Nrf2 was suppressed by the DAT inhibitor. Quinone formation and reduction of mesencephalic DA neurons after DA exposure were ameliorated by preincubation with conditioned media from DA-treated astrocytes. These protective effects were abrogated by MT-1/-2-specific antibody. Adding exogenous MT-1 to glial conditioned media also showed similar neuroprotective effects. Furthermore, MT-1/-2 expression was markedly elevated specifically in reactive astrocytes in the striatum of L-DOPA-treated hemi-parkinsonian mice or METH-injected mice. These results suggested that excess DA taken up by astrocytes via DAT upregulates MT-1/-2 expression specifically in astrocytes, and that MTs or related molecules secreted specifically by astrocytes protect dopaminergic neurons from damage through quinone quenching and/or scavenging of free radicals. (C)2010 Wiley-Liss, Inc.

    DOI: 10.1002/glia.21112

    Web of Science

    researchmap

  • Neuroprotective Effects of Zonisamide Target Astrocyte Reviewed

    Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Naotaka Kimoto, Yuri Kikkawa, Mika Takeshima, Ko Miyoshi, Miho Murata

    ANNALS OF NEUROLOGY   67 ( 2 )   239 - 249   2010.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-LISS  

    Objective: Recent double-blind, controlled trials in Japan showed that the antiepileptic agent zonisamide (ZNS)improves the cardinal symptoms of Parkinson&apos;s disease. Glutathione (GSH) exerts antioxidative activity through quenching reactive oxygen species and dopamine quinone. GSH depletion within dopaminergic neurons impairs mitochondrial complex I activity, followed by age-dependent nigrostriatal neurodegeneration. This study examined changes in GSH and GSH synthesis-related molecules, and the neuroprotective effects of ZNS on dopaminergic neurodegeneration using 6-hydroxydopamine-injected hemiparkinsonian mice brain and cultured neurons or astrocytes.
    Methods and Results: ZNS increased both the cell number and GSH levels in astroglial C6 cells, but not in dopaminergic neuronal CATH.a cells. Repeated injections of ZNS (30mg/kg intraperitoneally) for 14 days also significantly increased GSH levels and S100 beta-positive astrocytes in mouse basal ganglia. Repeated ZNS injections (30mg/kg) for 7 days in the hemiparkinsonian mice increased the expression of cystine/glutamate exchange transporter xCT in activated astrocytes, which supply cysteine to neurons for GSH synthesis. Treatment of these mice with ZNS also increased GSH levels and completely suppressed striatal levodopa-induced quinone formation. Reduction of nigrostriatal dopamine neurons in the lesioned side of hemiparkinsonian mice was significantly abrogated by repeated injections of ZNS with or without adjunctive levodopa starting 3 weeks after 6-hydroxydopamine lesioning.
    Interpretation: These results provide new pharmacological evidence for the effects of ZNS. ZNS markedly increased GSH levels by enhancing the astroglial cystine transport system and/or astroglial proliferation via S100 beta production or secretion. ZNS acts as a neuroprotectant against oxidative stress and progressive dopaminergic neurodegeneration. ANN NEUROL 2010;67:239-249

    DOI: 10.1002/ana.21885

    Web of Science

    researchmap

  • Approaches to Prevent Dopamine Quinone-Induced Neurotoxicity Reviewed

    Ikuko Miyazaki, Masato Asanuma

    NEUROCHEMICAL RESEARCH   34 ( 4 )   698 - 706   2009.4

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publisher:SPRINGER/PLENUM PUBLISHERS  

    Dopamine (DA) and its metabolites containing two hydroxyl residues exert cytotoxicity in dopaminergic neuronal cells, primarily due to the generation of highly reactive DA and DOPA quinones. Quinone formation is closely linked to other representative hypotheses such as mitochondrial dysfunction, inflammation, oxidative stress, and dysfunction of the ubiquitin-proteasome system, in the pathogenesis of neurodegenerative diseases such as Parkinson&apos;s disease and methamphetamine-induced neurotoxicity. Therefore, pathogenic effects of the DA quinone have focused on dopaminergic neuron-specific oxidative stress. Recently, various studies have demonstrated that some intrinsic molecules and several drugs exert protective effects against DA quinone-induced damage of dopaminergic neurons. In this article, we review recent studies on some neuroprotective approaches against DA quinone-induced dysfunction and/or degeneration of dopaminergic neurons.

    DOI: 10.1007/s11064-008-9843-1

    Web of Science

    researchmap

  • Dopaminergic neuron-specific oxidative stress caused by dopamine itself

    Ikuko Miyazaki, Masato Asanuma

    ACTA MEDICA OKAYAMA   62 ( 3 )   141 - 150   2008.6

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publisher:OKAYAMA UNIV MED SCHOOL  

    Oxidative stress, including the reactive oxygen or nitrogen species generated in the enzymatical oxidation or auto-oxidation of an excess amount of dopamine, is thought to play an important role in dopaminergic neurotoxicity. Dopamine and its metabolites containing 2 hydroxyl residues exert cytotoxicity in dopaminergic neuronal cells, primarily due to the generation of highly reactive dopamine and DOPA quinones. Dopamine and DOPA quinones may irreversibly alter protein function through the formation of 5-cysteinyl-catechols on the proteins. Furthermore, the quinone formation is closely linked to other representative hypotheses such as mitochondrial dysfunction, inflammation, oxidative stress, and dysfunction of the ubiquitin-proteasome system, in the pathogenesis of neurodegenerative diseases. Therefore, pathogenic effects of the dopamine quinone have recently focused on dopaminergic neuron-specific oxidative stress. In this article, we primarily review recent studies on the pathogenicity of quinone formation, in addition to several neuroprotective approaches against dopamine quinone-induced dysfunction of dopaminergic neurons.

    DOI: 10.18926/AMO/30980

    Web of Science

    researchmap

  • Protective effects of metallothionein against dopamine quinone-induced dopaminergic neurotoxicity Reviewed

    Ikuko Miyazaki, Masato Asanuma, Hiroaki Hozumi, Ko Miyoshi, Norio Sogawa

    FEBS LETTERS   581 ( 25 )   5003 - 5008   2007.10

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    Dopamine (DA) quinone as DA neuron-specific oxidative stress conjugates with cysteine residues in functional proteins to form quinoproteins. Here, we examined the effects of cysteine-rich metal-binding proteins, metallothionein (NIT)-1 and -2, on DA quinone-induced neurotoxicity. NIT quenched DA semiquinones in vitro. In dopaminergic cells, DA exposure increased quinoproteins and decreased cell viability; these were ameliorated by pretreatment with MT-inducer zinc. Repeated L-DOPA administration markedly elevated striatal quinoprotein levels and reduced the DA nerve terminals specifically on the lesioned side in NIT-knockout parkinsonian mice, but not in wild-type mice. Our results suggested that intrinsic NIT protects against L-DOPA-induced DA quinone neurotoxicity in parkinsonian mice by its quinone-quenching property. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.febslet.2007.09.046

    Web of Science

    researchmap

  • Methamphetamine‐induced dopaminergic neurotoxicity is regulated by quinone formation‐related molecules Reviewed

    Ikuko Miyazaki, Masato Asanuma, Francisco J. Diaz‐Corrales, Masaya Fukuda, Kiyoyuki Kitaichi, Ko Miyoshi, Norio Ogawa

    The FASEB Journal   20 ( 3 )   571 - 573   2006.3

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1096/fj.05-4996fje

    Web of Science

    researchmap

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.05-4996fje

  • Dopamine agonist pergolide prevents levodopa-induced quinoprotein formation in Parkinsonian striatum and shows quenching effects on dopamine-semiquinone generated in vitro Reviewed

    Ikuko Miyazaki, Masato Asanuma, Francisco J. Diaz-Corrales, Ko Miyoshi, Norio Ogawa

    Clinical Neuropharmacology   28 ( 4 )   155 - 160   2005.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The neurotoxicity of dopamine (DA) quinones that appears in dopaminergic neuron-specific oxidative stress has recently been shown to play a role in the pathogenesis and/or progression of Parkinson disease. To clarify the effects of a DA agonist, pergolide, on the levodopa-induced elevation of quinones, the authors examined, striatal changes in quinoprotein using a hemi-parkinsonian mouse model. The level of striatal quinoprotein was significantly elevated specifically on the parkinsonian side, but not on the control side, after repeated levodopa administration. This levodopa-induced increase in striatal quinoprotein was almost completely suppressed by adjunctive administration with pergolide on the lesioned side. Furthermore, it was clarified that pergolide scavenged DA-semiquinones generated in vitro in a dose-dependent manner. These suppressive and quenching effects of pergolide against cytotoxic DA quinones may play a key role in its neuroprotective mechanism in the parkinsonian brain. Copyright © 2005 by Lippincott Williams &amp
    Wilkins.

    DOI: 10.1097/01.wnf.0000175523.33334.24

    Scopus

    PubMed

    researchmap

  • Direct evidence for expression of dopamine receptors in astrocytes from basal ganglia Reviewed

    Ikuko Miyazaki, Masato Asanuma, Francisco J. Diaz-Corrales, Ko Miyoshi, Norio Ogawa

    Brain Research   1029 ( 1 )   120 - 123   2004.12

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Expression of dopamine receptors (DA-Rs) in astrocytes was examined in vitro and in vivo using primary cultured astrocytes and brain slices from rat basal ganglia. Astrocytes from basal ganglia expressed DA D1-, D3-, D4- and D5-receptors and D4-mediated signal transduction in response to DA, suggesting possible involvement of astrocytes in the pharmacological action of atypical antipsychotic drugs and in DA response in some neurological diseases. © 2004 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.brainres.2004.09.014

    Web of Science

    Scopus

    PubMed

    researchmap

  • Age-related changes in expression of metallothionein-III in rat brain Reviewed

    Ikuko Miyazaki, Masato Asanuma, Youichirou Higashi, Chiharu Aoki Sogawa, Ken-ichi Tanaka, Norio Ogawa

    Neuroscience Research   43 ( 4 )   323 - 333   2002.8

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0168-0102(02)00057-3

    Web of Science

    researchmap

  • Expression of metallothionein-III mRNA and its regulation by levodopa in the basal ganglia of hemi-parkinsonian rats Reviewed

    Ikuko Miyazaki, Chiharu Aoki Sogawa, Masato Asanuma, Youichirou Higashi, Ken-ichi Tanaka, Tohru Nakanishi, Norio Ogawa

    Neuroscience Letters   293 ( 1 )   65 - 68   2000.10

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0304-3940(00)01488-9

    Web of Science

    researchmap

  • Bifemelane hydrochloride protects against cytotoxicity of hydrogen peroxide on cultured rat neuroblastoma cell line Reviewed

    Ikuko Miyazaki, Emi Iwata-Ichikawa, Masato Asanuma, Motoyuki Iida, Norio Ogawa

    Neurochemical Research   24 ( 7 )   857 - 860   1999

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1023/a:1020953913490

    Web of Science

    researchmap

  • Neuroprotective Effects of Anti-high Mobility Group Box-1 Monoclonal Antibody Against Methamphetamine-Induced Dopaminergic Neurotoxicity Reviewed

    Kaori Masai, Keita Kuroda, Nami Isooka, Ryo Kikuoka, Shinki Murakami, Sunao Kamimai, Dengli Wang, Keyue Liu, Ikuko Miyazaki, Masahiro Nishibori, Masato Asanuma

    Neurotoxicity Research   39 ( 5 )   1511 - 1523   2021.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s12640-021-00402-5

    researchmap

    Other Link: https://link.springer.com/article/10.1007/s12640-021-00402-5/fulltext.html

  • Influence of 5-HT2A receptor function on anxiety-like behavior induced by a combination treatment with doxorubicin and cyclophosphamide in rats Reviewed

    Hironori Tabuchi, Yoshihisa Kitamura, Soichiro Ushio, Shiho Kan, Yudai Wada, Yusuke Sumiyoshi, Yasuhisa Izushi, Ikuko Miyazaki, Masato Asanuma, Toshiaki Sendo

    Psychopharmacology   238 ( 12 )   3607 - 3614   2021.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00213-021-05979-5

    PubMed

    researchmap

    Other Link: https://link.springer.com/article/10.1007/s00213-021-05979-5/fulltext.html

  • Glutathione and Related Molecules in Parkinsonism Invited Reviewed

    Masato Asanuma, Ikuko Miyazaki

    International Journal of Molecular Sciences   22 ( 16 )   8689 - 8689   2021.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Glutathione (GSH) is the most abundant intrinsic antioxidant in the central nervous system, and its substrate cysteine readily becomes the oxidized dimeric cystine. Since neurons lack a cystine transport system, neuronal GSH synthesis depends on cystine uptake via the cystine/glutamate exchange transporter (xCT), GSH synthesis, and release in/from surrounding astrocytes. Transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2), a detoxifying master transcription factor, is expressed mainly in astrocytes and activates the gene expression of various phase II drug-metabolizing enzymes or antioxidants including GSH-related molecules and metallothionein by binding to the antioxidant response element (ARE) of these genes. Accumulating evidence has shown the involvement of dysfunction of antioxidative molecules including GSH and its related molecules in the pathogenesis of Parkinson’s disease (PD) or parkinsonian models. Furthermore, we found several agents targeting GSH synthesis in the astrocytes that protect nigrostriatal dopaminergic neuronal loss in PD models. In this article, the neuroprotective effects of supplementation and enhancement of GSH and its related molecules in PD pathology are reviewed, along with introducing new experimental findings, especially targeting of the xCT-GSH synthetic system and Nrf2–ARE pathway in astrocytes.

    DOI: 10.3390/ijms22168689

    researchmap

  • N-Acetylcysteine Attenuates the Anxiety-Like Behavior and Spatial Cognition Impairment Induced by Doxorubicin and Cyclophosphamide Combination Treatment in Rats Reviewed

    Yoshihisa Kitamura, Soichiro Ushio, Yusuke Sumiyoshi, Yudai Wada, Ikuko Miyazaki, Masato Asanuma, Toshiaki Sendo

    Pharmacology   106 ( 5-6 )   286 - 293   2021

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:S. Karger AG  

    &lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Cancer patients can suffer from psychological and cognitive disorders after chemotherapy, which influence quality of life. &lt;b&gt;&lt;i&gt;Objective:&lt;/i&gt;&lt;/b&gt; Oxidative stress may contribute to the psychological and cognitive disorders induced in rats by chemotherapy. In the present study, we examined the effects of N-acetylcysteine, an anti-oxidant, on anxiety-like behavior and cognitive impairment in rats treated with a combination of doxorubicin and cyclophosphamide. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Rats were intraperitoneally injected with doxorubicin and cyclophosphamide once a week for 2 weeks. The light-dark test and the novel location recognition test were used to assess anxiety-like behavior and spatial cognition, respectively. The rats’ hippocampal levels of glutathione (GSH) and glutathione disulfide (GSSG) were measured using a GSSG/GSH quantification kit. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Combined treatment with doxorubicin and cyclophosphamide produced anxiety-like behavior and cognitive impairment in rats. N-acetylcysteine reversed the anxiety-like behavior and inhibition of novel location recognition induced by the combination treatment. Furthermore, the combination of doxorubicin and cyclophosphamide significantly reduced the rats’ hippocampal GSH/GSSG ratios. N-acetylcysteine reversed the reduction in the GSH/GSSG ratio seen in the doxorubicin and cyclophosphamide-treated rats. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; These results suggest that N-acetylcysteine inhibits doxorubicin and cyclophosphamide-induced anxiety-like behavior and cognitive impairment by reducing oxidative stress in the hippocampus.

    DOI: 10.1159/000512117

    researchmap

  • Glial cells as a target of neuroprotection in the central and peripheral nervous system in Parkinson’s disease. Invited Reviewed

    Nami Isooka, Ikuko Miyazaki, Masato Asanuma

    75 ( 5 )   549 - 556   2021

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    researchmap

  • The neurotoxicity of psychoactive phenethylamines “2C series” in cultured monoaminergic neuronal cell lines Reviewed

    Masato Asanuma, Ikuko Miyazaki, Masahiko Funada

    Forensic Toxicology   38 ( 2 )   394 - 408   2020.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s11419-020-00527-w

    researchmap

    Other Link: http://link.springer.com/article/10.1007/s11419-020-00527-w/fulltext.html

  • Cerebellar Blood Flow and Gene Expression in Crossed Cerebellar Diaschisis after Transient Middle Cerebral Artery Occlusion in Rats Reviewed

    Naoya Kidani, Tomohito Hishikawa, Masafumi Hiramatsu, Shingo Nishihiro, Kyohei Kin, Yu Takahashi, Satoshi Murai, Kenji Sugiu, Takao Yasuhara, Ikuko Miyazaki, Masato Asanuma, Isao Date

    International Journal of Molecular Sciences   21 ( 11 )   4137 - 4137   2020.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Crossed cerebellar diaschisis (CCD) is a state of hypoperfusion and hypometabolism in the contralesional cerebellar hemisphere caused by a supratentorial lesion, but its pathophysiology is not fully understood. We evaluated chronological changes in cerebellar blood flow (CbBF) and gene expressions in the cerebellum using a rat model of transient middle cerebral artery occlusion (MCAO). CbBF was analyzed at two and seven days after MCAO using single photon emission computed tomography (SPECT). DNA microarray analysis and western blotting of the cerebellar cortex were performed and apoptotic cells in the cerebellar cortex were stained. CbBF in the contralesional hemisphere was significantly decreased and this lateral imbalance recovered over one week. Gene set enrichment analysis revealed that a gene set for “oxidative phosphorylation” was significantly upregulated while fourteen other gene sets including “apoptosis”, “hypoxia” and “reactive oxygen species” showed a tendency toward upregulation in the contralesional cerebellum. MCAO upregulated the expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in the contralesional cerebellar cortex. The number of apoptotic cells increased in the molecular layer of the contralesional cerebellum. Focal cerebral ischemia in our rat MCAO model caused CCD along with enhanced expression of genes related to oxidative stress and apoptosis.

    DOI: 10.3390/ijms21114137

    PubMed

    researchmap

  • Attenuation of Macrophage Migration Inhibitory Factor-Stimulated Signaling via S-Nitrosylation Reviewed

    Kengo Nakahara, Kana Fujikawa, Hideki Hiraoka, Ikuko Miyazaki, Masato Asanuma, Akihiro Ito, Nobumasa Takasugi, Takashi Uehara

    Biological and Pharmaceutical Bulletin   42 ( 6 )   1044 - 1047   2019.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Pharmaceutical Society of Japan  

    DOI: 10.1248/bpb.b19-00025

    PubMed

    researchmap

  • Involvement of 5-HT2A receptor hyperfunction in the anxiety-like behavior induced by doxorubicin and cyclophosphamide combination treatment in rats. Reviewed

    Nakamura Y, Kitamura Y, Sumiyoshi Y, Naito N, Kan S, Ushio S, Miyazaki I, Asanuma M, Sendo T

    Journal of pharmacological sciences   138 ( 3 )   192 - 197   2018.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We examined whether combination treatment with doxorubicin and cyclophosphamide, a traditional chemotherapy for breast cancer, induced anxiety-like behavior in rats. Furthermore, we evaluated the role of the serotonin (5-HT)2A receptor subtype in the anxiety-like behavior induced by such chemotherapy. Rats were intraperitoneally injected with doxorubicin and cyclophosphamide once a week for 2 weeks. This caused the rats to display anxiety-like behavior during the light-dark test. In addition, we examined the rats' 5-HT2A receptor-mediated behavioral responses. Combination treatment with doxorubicin and cyclophosphamide significantly increased (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane, (a 5-HT2A receptor agonist)-induced wet-dog shake activity. This anxiety-like behavior was significantly inhibited by mirtazapine, a 5-HT2A receptor antagonist/5-HT1A receptor agonist, and tandospirone, a partial 5-HT1A receptor agonist, but not by fluoxetine, a selective serotonin reuptake inhibitor. The anxiety-like behavior induced by doxorubicin and cyclophosphamide combination treatment is mediated by hyperfunctioning of the 5-HT2A receptor. Thus, 5-HT2A receptor antagonists or 5-HT1A receptor agonists might be useful for treating chemotherapy-induced anxiety disorders.

    DOI: 10.1016/j.jphs.2018.10.001

    Scopus

    PubMed

    researchmap

    Other Link: http://orcid.org/0000-0002-3652-3722

  • Neuroprotective effect of fermented papaya preparation by activation of Nrf2 pathway in astrocytes Reviewed

    Shinki Murakami, Ikuko Miyazaki, Masato Asanuma

    Nutritional Neuroscience   21 ( 3 )   176 - 184   2018.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1080/1028415x.2016.1253171

    Scopus

    researchmap

  • Influence of nicotine on doxorubicin and cyclophosphamide combination treatment-induced spatial cognitive impairment and anxiety-like behavior in rats Reviewed

    Yoshihisa Kitamura, Erika Kanemoto, Misaki Sugimoto, Ayumi Machida, Yuka Nakamura, Nanami Naito, Hirotaka Kanzaki, Ikuko Miyazaki, Masato Asanuma, Toshiaki Sendo

    Naunyn-Schmiedeberg's Archives of Pharmacology   390 ( 4 )   369 - 378   2017.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00210-016-1338-z

    Web of Science

    researchmap

    Other Link: http://link.springer.com/article/10.1007/s00210-016-1338-z/fulltext.html

  • 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA Reviewed

    Masato Asanuma, Ikuko Miyazaki

    BMC Neuroscience   17 ( 1 )   2016.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1186/s12868-016-0289-0

    Web of Science

    researchmap

    Other Link: http://link.springer.com/article/10.1186/s12868-016-0289-0/fulltext.html

  • In vitro quantitative determination of the concentration of the polymerization agent methyl 2-benzoylbenzoate in intravenous injection solution and the cytotoxic effects of the chemical on normal human peripheral blood mononuclear cells Reviewed

    Tsuboi C, Kawasaki Y, Yoshitome K, Yagi K, Miura T, Esumi S, Miyazaki I, Asanuma M, Kitamura Y, Sendo T

    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH   23 ( 10 )   10262 - 10269   2016.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER HEIDELBERG  

    In previous studies, we detected the photoinitiators 1-hydroxycyclohexyl phenyl ketone (1-HCHPK) and 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous injection solution. Importantly, 1-HCHPK and MTMP have been demonstrated to be cytotoxic to normal human peripheral blood (PB) mononuclear cells (MNC). Cell death (apoptosis) pathways can be classified into two modes, caspase-dependent and -independent pathways. However, it is unclear whether methyl 2-benzoylbenzoate (MBB) induces the caspase-dependent and/or -independent pathway in normal human PBMNC. In the present in vitro study, we examined the levels of MBB in a solution from an intravenous fluid bag and the cytotoxicity of MBB towards normal human PBMNC via the caspase-8-, caspase-9-, or apoptosis-inducing factor (AIF)-mediated apoptosis pathways. We found that extracts from the injection solution had been contaminated with approximately 80 mu M of the photoinitiator MBB. In addition, MBB induced apoptosis in the high concentration range in normal human PBMNC in vitro. Moreover, we found that MBB-induced apoptosis occurs via the caspase-9 pathway, but not the AIF pathway. In conclusion, we suggest that MBB has cytotoxic effects on normal human PBMNC in vitro, which are mediated via the caspase-dependent pathway.

    DOI: 10.1007/s11356-016-6332-y

    Web of Science

    researchmap

  • Anti-high mobility group box 1 antibody exerts neuroprotection in a rat model of Parkinson's disease Reviewed

    Tatsuya Sasaki, Keyue Liu, Takashi Agari, Takao Yasuhara, Jun Morimoto, Mihoko Okazaki, Hayato Takeuchi, Atsuhiko Toyoshima, Susumu Sasada, Aiko Shinko, Akihiko Kondo, Masahiro Kameda, Ikuko Miyazaki, Masato Asanuma, Cesario V. Borlongan, Masahiro Nishibori, Isao Date

    EXPERIMENTAL NEUROLOGY   275 ( 1 )   220 - 231   2016.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The high mobility group box-1 (HMGB1) exists as an architectural nuclear protein in the normal state, but displays an inflammatory cytokine-like activity in the extracellular space under pathological condition. Inflammation in the pathogenesis of Parkinson's disease (PD) has been documented. In this study, we investigated the involvement of HMGB1 in the pathology and the neuroprotective effects of neutralizing anti-HMGB1 monoclonal antibody (mAb) on an animal model of PD. Adult female Sprague-Dawley rats were initially injected with 6-hydroxydopmaine (6-OHDA, 20 mu g/4 mu l) into the right striatum, then anti-HMGB1 mAb (1 mg/kg), or control mAb was intravenously administered immediately, at 6 and 24 h after 6-OHDA injection. The treatment with anti-HMGB1 mAb significantly preserved dopaminergic neurons in substantia nigra pars compacta and dopaminergic terminals inherent in the striatum, and attenuated PD behavioral symptoms compared to the control mAb-treated group. HMGB1 was retained in the nucleus of neurons and astrocytes by inhibiting the proinflammation-induced oxidative stress in the anti-HMGB1 mAb-treated group, whereas HMGB1 translocation was observed in neurons at 1 day and astrocytes at 7 days after 6-OHDA injection in the control mAb-treated group. Anti-HMGB1 mAb inhibited the activation of microglia, disruption of blood-brain-barrier (BBB), and the expression of inflammation cytokines such as IL-1 beta. and IL-6. These results suggested that HMGB1 released from neurons and astrocytes was at least partly involved in the mechanism and pathway of degeneration of dopaminergic neurons induced by 6-0HDA exposure. Intravenous administration of anti-HMGB1 mAb stands as a novel therapy for PD possibly acting through the suppression of neuroinflammation and the attenuation of disruption of BOB associated with the disease. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.expneurol.2015.11.003

    Web of Science

    researchmap

  • L-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes Reviewed

    Mika Takeshima, Ikuko Miyazaki, Shinki Murakami, Taizo Kita, Masato Asanuma

    Journal of Clinical Biochemistry and Nutrition   59 ( 2 )   93 - 99   2016

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Society for Free Radical Research Japan  

    <p><span style="font-variant: small-caps;">l</span>-Theanine (γ-glutamylethylamide), a component of green tea, is considered to have regulatory and neuroprotective roles in the brain. The present study was designed to determine the effect of <span style="font-variant: small-caps;">l</span>-theanine on excess dopamine-induced neurotoxicity in both cell culture and animal experiments. The primary cultured mesencephalic neurons or co-cultures of mesencephalic neurons and striatal astrocytes were pretreated with <span style="font-variant: small-caps;">l</span>-theanine for 72 h, and then treated with excess dopamine for further 24 h. The cell viability of dopamine neurons and levels of glutathione were evaluated. Excess dopamine-induced neurotoxicity was significantly attenuated by 72 h preincubation with <span style="font-variant: small-caps;">l</span>-theanine in neuron-astrocyte co-cultures but not in neuron-rich cultures. Exposure to <span style="font-variant: small-caps;">l</span>-theanine increased the levels of glutathione in both astrocytes and glial conditioned medium. The glial conditioned medium from <span style="font-variant: small-caps;">l</span>-theanine-pretreated striatal astrocytes attenuated dopamine-induced neurotoxicity and quinoprotein formation in mesencephalic neurons. In addition, replacement of <span style="font-variant: small-caps;">l</span>-glutamate with <span style="font-variant: small-caps;">l</span>-theanine in an in vitro cell-free glutathione-synthesis system produced glutathione-like thiol compounds. Furthermore, <span style="font-variant: small-caps;">l</span>-theanine administration (4 mg/kg, p.o.) for 14 days significantly increased glutathione levels in the striatum of mice. The results suggest that <span style="font-variant: small-caps;">l</span>-theanine provides neuroprotection against oxidative stress-induced neuronal damage by humoral molecules released from astrocytes, probably including glutathione.</p>

    DOI: 10.3164/jcbn.16-15

    Web of Science

    CiNii Article

    researchmap

  • Changes in Metallothionein Isoform Expression in the Bones of Ovariectomized Rats Reviewed

    Chiharu Sogawa, Mika Ikegame, Ikuko Miyazaki, Toshiaki Ara, Yasuhiro Imamura, Yuka Okusha, Kazumi Ohyama, Masato Asanuma, Norio Sogawa, Toshio Yamamoto, Ken-ichi Kozaki

    Journal of Hard Tissue Biology   25 ( 1 )   21 - 26   2016

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Society for Hard Tissue Regenerative Biology  

    DOI: 10.2485/jhtb.25.21

    Web of Science

    researchmap

  • Doxorubicin and cyclophosphamide treatment produces anxiety-like behavior and spatial cognition impairment in rats: Possible involvement of hippocampal neurogenesis via brain-derived neurotrophic factor and cyclin D1 regulation Reviewed

    Yoshihisa Kitamura, Sayo Hattori, Saori Yoneda, Saori Watanabe, Erika Kanemoto, Misaki Sugimoto, Toshiki Kawai, Ayumi Machida, Hirotaka Kanzaki, Ikuko Miyazaki, Masato Asanuma, Toshiaki Sendo

    BEHAVIOURAL BRAIN RESEARCH   292   184 - 193   2015.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    Many patients who have received chemotherapy to treat cancer experience depressive- and anxiety-like symptoms or cognitive impairment. However, despite the evidence for this, the underlying mechanisms are still not understood. This study investigated behavioral and biochemical changes upon treatment with doxorubicin and cyclophosphamide, focusing on mental and cognitive systems, as well as neurogenesis in male rats. Doxorubicin (2 mg/kg), cyclophosphamide (50 mg/kg), and the combination of doxorubicin and cyclophosphamide were injected intraperitoneally once per week for 4 weeks. In particular, the co-administration of doxorubicin and cyclophosphamide produced anhedonia-like, anxiety-like, and spatial cognitive impairments in rats. It also reduced both the number of proliferating cells in the subgranular zone of the hippocampal dentate gyrus and their survival. Serum brain-derived neurotrophic factor (BDNF) levels were decreased along with chemotherapy-induced decreases in platelet levels. However, hippocampal BDNF levels and Bdnf mRNA levels were not decreased by this treatment. On the other hand, hippocampal cyclin D1 levels were significantly decreased by chemotherapy. These results suggest that the co-administration of doxorubicin and cyclophosphamide induces psychological and cognitive impairment, in addition to negatively affecting hippocampal neurogenesis, which may be related to hippocampal cyclin D1 levels, but not hippocampal BDNF levels. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.bbr.2015.06.007

    Web of Science

    researchmap

  • Long-Term Systemic Exposure to Rotenone Induces Central and Peripheral Pathology of Parkinson's Disease in Mice Reviewed

    Shinki Murakami, Ikuko Miyazaki, Ko Miyoshi, Masato Asanuma

    NEUROCHEMICAL RESEARCH   40 ( 6 )   1165 - 1178   2015.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER/PLENUM PUBLISHERS  

    Parkinson's disease (PD) is a progressive neurodegenerative disease with motor and non-motor symptoms that precede the onset of motor symptoms. Rotenone is often used to induce PD-like pathology in the central nervous system (CNS) and enteric nervous system (ENS). However, there is little or no information on the temporal changes in other neural tissues and the spread of pathology throughout the entire body organs. Here, we recorded the serial immunohistochemical changes in neurons and glial cells of the striatum, substantia nigra (SN), olfactory bulb (OB), thoracic cord (ThC) and ascending colon (AC) induced by 1-, 3- and 6-week administration of rotenone (50 mg/kg/day) infused subcutaneously in C57BL mice using an osmotic pump. Rotenone exposure for 3 or 6 weeks caused neurodegeneration in the striatum, whereas neuronal damage was seen in the SN and OB only after 6 weeks. Moreover, rotenone induced neurodegeneration in the myenteric plexus of AC but not in ThC. Rotenone also activated glial cells before any apparent neurodegeneration in the CNS but not in the ENS. Our results demonstrated that subcutaneous administration of rotenone can cause progressive neurodegeneration in the OB and AC, in addition to the nigrostriatal pathway, and temporal differential glial activation, and that these changes do not spread retrogradely from OB or ENS to nigrostriatal pathway. The results suggested that the different vulnerability of neurons to the neurotoxic effects of rotenone administrated subcutaneously are due to glial activation in these neural tissues.

    DOI: 10.1007/s11064-015-1577-2

    Web of Science

    researchmap

  • ハルミンの薬物依存性ならびに細胞毒性の評価:植物由来幻覚成分の有害作用について.

    舩田正彦, 竹林美佳, 宮崎育子, 浅沼幹人, 青尾直也, 和田 清

    精神保健研究   61 ( 28 )   61 - 72   2015

     More details

  • Visualization of Astrocytic Primary Cilia in the Mouse Brain by Immunofluorescent Analysis Using the Cilia Marker Arl13b Reviewed

    Kyosuke Kasaharaa, Ko Miyoshi, Shinki Murakami, Ikuko Miyazaki, Masato Asanuma

    ACTA MEDICA OKAYAMA   68 ( 6 )   317 - 322   2014.12

     More details

    Language:English   Publisher:OKAYAMA UNIV MED SCHOOL  

    In vertebrates, almost all somatic cells extend a single immotile cilium, referred to as a primary cilium. Increasing evidence suggests that primary cilia serve as cellular antennae in many types of tissues by sensing chemical or mechanical stimuli in the milieu surrounding the cells. In rodents an antibody to adenylyl cyclase 3 (AC3) has been widely used to label the primary cilia of neurons in vivo by immunostaining, whereas the lack of markers for the primary cilia of astrocytes has made it difficult to observe astrocytic primary cilia in vivo. Here, we obtained a visualization of astrocytic primary cilia in the mouse brain. In the somatosensory cortex, a large portion of neurons and astrocytes at postnatal day 10 (P10), and of neurons at P56 had AC3-positive primary cilia, whereas only approx. one-half of the astrocytes in the P56 mice carried primary cilia weakly positive for AC3. In contrast, the majority of astrocytes had ADP-ribosylation factor-like protein 13B (Arl13b)-positive primary cilia in the somatosensory cortex and other brain regions of P56 mice. The lengths of astrocytic primary cilia positive for Arl13b varied among the brain regions. Our data indicate that Arl13b is a noteworthy marker of astrocytic primary cilia in the brain.

    DOI: 10.18926/AMO/53020

    Web of Science

    researchmap

  • Neuroprotective Effects of Metallothionein Against Rotenone-Induced Myenteric Neurodegeneration in Parkinsonian Mice Reviewed

    Shinki Murakami, Ikuko Miyazaki, Norio Sogawa, Ko Miyoshi, Masato Asanuma

    NEUROTOXICITY RESEARCH   26 ( 3 )   285 - 298   2014.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Parkinson's disease (PD) is a neurodegenerative disease with motor symptoms as well as non-motor symptoms that precede the onset of motor symptoms. Mitochondrial complex I inhibitor, rotenone, has been widely used to reproduce PD pathology in the central nervous system (CNS) and enteric nervous system (ENS). We reported previously that metallothioneins (MTs) released from astrocytes can protect dopaminergic neurons against oxidative stress. The present study examined the changes in MT expression by chronic systemic rotenone administration in the striatum and colonic myenteric plexus of C57BL mice. In addition, we investigated the effects of MT depletion on rotenone-induced neurodegeneration in CNS and ENS using MT-1 and MT-2 knockout (MT KO) mice, or using primary cultured neurons from MT KO mice. In normal C57BL mice, subcutaneous administration of rotenone for 6 weeks caused neurodegeneration, increased MT expression with astrocytes activation in the striatum and myenteric plexus. MT KO mice showed more severe myenteric neuronal damage by rotenone administration after 4 weeks than wild-type mice, accompanied by reduced astroglial activation. In primary cultured mesencephalic neurons from MT KO mice, rotenone exposure induced neurotoxicity in dopaminergic neurons, which was complemented by addition of recombinant protein. The present results suggest that MT seems to provide protection against neurodegeneration in ENS of rotenone-induced PD model mice.

    DOI: 10.1007/s12640-014-9480-1

    Web of Science

    researchmap

  • Methylphenidate improves learning impairments and hyperthermia-induced seizures caused by an Scn1a mutation Reviewed

    Iori Ohmori, Nozomi Kawakami, Sumei Liu, Haijiao Wang, Ikuko Miyazaki, Masato Asanuma, Hiroyuki Michiue, Hideki Matsui, Tomoji Mashimo, Mamoru Ouchida

    EPILEPSIA   55 ( 10 )   1558 - 1567   2014.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    ObjectiveDevelopmental disorders including cognitive deficit, hyperkinetic disorder, and autistic behaviors are frequently comorbid in epileptic patients with SCN1A mutations. However, the mechanisms underlying these developmental disorders are poorly understood and treatments are currently unavailable. Using a rodent model with an Scn1a mutation, we aimed to elucidate the pathophysiologic basis and potential therapeutic treatments for developmental disorders stemming from Scn1a mutations.MethodsWe conducted behavioral analyses on rats with the N1417H-Scn1a mutation. With high-performance liquid chromatography, we measured dopamine and its metabolites in the frontal cortex, striatum, nucleus accumbens, and midbrain. Methylphenidate was administered intraperitoneally to examine its effects on developmental disorder-like behaviors and hyperthermia-induced seizures.ResultsBehavioral studies revealed that Scn1a-mutant rats had repetitive behavior, hyperactivity, anxiety-like behavior, spatial learning impairments, and motor imbalance. Dopamine levels in the striatum and nucleus accumbens in Scn1a-mutant rats were significantly lower than those in wild-type rats. In Scn1a-mutant rats, methylphenidate, by increasing dopamine levels in the synaptic cleft, improved hyperactivity, anxiety-like behavior, and spatial learning impairments. Surprisingly, methylphenidate also strongly suppressed hyperthermia-induced seizures.SignificanceDysfunction of the mesolimbic dopamine reward pathway may contribute to the hyperactivity and learning impairments in Scn1a-mutant rats. Methylphenidate was effective for treating hyperactivity, learning impairments, and hyperthermia-induced seizures. We propose that methylphenidate treatment may ameliorate not only developmental disorders but also epileptic seizures in patients with SCN1A mutations.

    DOI: 10.1111/epi.12750

    Web of Science

    researchmap

  • Striatal Astrocytes Act as a Reservoir for L-DOPA Reviewed

    Masato Asanuma, Ikuko Miyazaki, Shinki Murakami, Francisco J. Diaz-Corrales, Norio Ogawa

    PLOS ONE   9 ( 9 )   e106362   2014.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    L-DOPA is therapeutically efficacious in patients with Parkinson's disease (PD), although dopamine (DA) neurons are severely degenerated. Since cortical astrocytes express neutral amino acid transporter (LAT) and DA transporter (DAT), the uptake and metabolism of L-DOPA and DA in striatal astrocytes may influence their availability in the dopaminergic system of PD. To assess possible L-DOPA-and DA-uptake and metabolic properties of striatal astrocytes, we examined the expression of L-DOPA, DA and DAT in striatal astrocytes of hemi-parkinsonian model rats after repeated L-DOPA administration, and measured the contents of L-DOPA, DA and their metabolite in primary cultured striatal astrocytes after L-DOPA/DA treatment. Repeated injections of L-DOPA induced apparent L-DOPA-and DA-immunoreactivities and marked expression of DAT in reactive astrocytes on the lesioned side of the striatum in hemi-parkinsonian rats. Exposure to DA for 4 h significantly increased the levels of DA and its metabolite DOPAC in cultured striatal astrocytes. L-DOPA was also markedly increased in cultured striatal astrocytes after 4-h L-DOPA exposure, but DA was not detected 4 or 8 h after L-DOPA treatment, despite the expression of aromatic amino acid decarboxylase in astrocytes. Furthermore, the intracellular level of L-DOPA in cultured striatal astrocytes decreased rapidly after removal of extracellular L-DOPA. The results suggest that DA uptaken into striatal astrocytes is rapidly metabolized and that striatal astrocytes act as a reservoir of L-DOPA that govern the uptake or release of L-DOPA depending on extracellular L-DOPA concentration, but are less capable of converting L-DOPA to DA.

    DOI: 10.1371/journal.pone.0106362

    Web of Science

    researchmap

  • Effects of (+)-8-OH-DPAT on the duration of immobility during the forced swim test and hippocampal cell proliferation in ACTH-treated rats Reviewed

    Ayaka Miyake, Yoshihisa Kitamura, Ikuko Miyazaki, Masato Asanuma, Toshiaki Sendo

    PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR   122   240 - 245   2014.7

     More details

    Language:English   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    In the present study, we examined the effect of ACTH on the immobilization of rats in the forced swim test and hippocampal cell proliferation after administration of the 5-HT1A receptor agonist, R-(+)-8-hydroxy-2-di-n-propylamino tetralin ((+)-8-OH-DPAT). Chronic treatment with (+)-8-OH-DPAT (0.01-0.1 mg/kg, s.c.) significantly decreased the duration of immobility in saline- and ACTH-treated rats. Chronic administration of ACTH caused a significant decrease in hippocampal cell proliferation. However, (+)-8-OH-DPAT significantly normalized cell proliferation in ACTH-treated rats. We then investigated the effects of (+)-8-OH-DPAT on the expression of brain-derived neurotrophic factor (BDNF) and cyclin D1 (elements of cyclic adenosine monophosphate response element-binding protein (CREB)-BDNF and Wnt signaling pathways, respectively) in the hippocampus of saline- and ACTH-treated rats. ACTH treatment significantly decreased the expression of cyclin D1, while treatment with (+)-8-OH-DPAT normalized the expression of cyclin D1 in ACTH-treated rats. However, the expression of BDNF did not change in either saline- or ACTH-treated rats. These findings suggest that the antidepressant effects of (+)-8-OH-DPAT in treatment-resistant animals may be attributed to an enhancement of hippocampal cell proliferation, at least in part due to an enhancement of cyclin D1 expression. (C) 2014 Elsevier Inc All rights reserved.

    DOI: 10.1016/j.pbb.2014.04.003

    Web of Science

    researchmap

  • Lack of Dopaminergic Inputs Elongates the Primary Cilia of Striatal Neurons Reviewed

    Ko Miyoshi, Kyosuke Kasahara, Shinki Murakami, Mika Takeshima, Natsuko Kumamoto, Asako Sato, Ikuko Miyazaki, Shinsuke Matsuzaki, Toshikuni Sasaoka, Taiichi Katayama, Masato Asanuma

    PLOS ONE   9 ( 5 )   e97918   2014.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    In the rodent brain, certain G protein-coupled receptors and adenylyl cyclase type 3 are known to localize to the neuronal primary cilium, a primitive sensory organelle protruding singly from almost all neurons. A recent chemical screening study demonstrated that many compounds targeting dopamine receptors regulate the assembly of Chlamydomonas reinhardtii flagella, structures which are analogous to vertebrate cilia. Here we investigated the effects of dopaminergic inputs loss on the architecture of neuronal primary cilia in the rodent striatum, a brain region that receives major dopaminergic projections from the midbrain. We first analyzed the lengths of neuronal cilia in the dorsolateral striatum of hemi-parkinsonian rats with unilateral lesions of the nigrostriatal dopamine pathway. In these rats, the striatal neuronal cilia were significantly longer on the lesioned side than on the non-lesioned side. In mice, the repeated injection of reserpine, a dopamine-depleting agent, elongated neuronal cilia in the striatum. The combined administration of agonists for dopamine receptor type 2 (D2) with reserpine attenuated the elongation of striatal neuronal cilia. Repeated treatment with an antagonist of D2, but not of dopamine receptor type 1 (D1), elongated the striatal neuronal cilia. In addition, D2-null mice displayed longer neuronal cilia in the striatum compared to wild-type controls. Reserpine treatment elongated the striatal neuronal cilia in D1-null mice but not in D2-null mice. Repeated treatment with a D2 agonist suppressed the elongation of striatal neuronal cilia on the lesioned side of hemi-parkinsonian rats. These results suggest that the elongation of striatal neuronal cilia following the lack of dopaminergic inputs is attributable to the absence of dopaminergic transmission via D2 receptors. Our results provide the first evidence that the length of neuronal cilia can be modified by the lack of a neurotransmitter's input.

    DOI: 10.1371/journal.pone.0097918

    Web of Science

    researchmap

  • Protective Effects of Phytochemical Antioxidants Against Neurotoxin-Induced Degeneration of Dopaminergic Neurons Reviewed

    Taizo Kita, Masato Asanuma, Ikuko Miyazaki, Mika Takeshima

    JOURNAL OF PHARMACOLOGICAL SCIENCES   124 ( 3 )   313 - 319   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    The specific toxicity to dopaminergic neurons of psychostimulants and neurotoxins has been extensively studied in vivo and in vitro, and findings have been used to establish animal models of amphetamine psychosis or Parkinson's disease. The multiple mechanisms of neurotoxicity operating in these disorders are known to involve oxidative stress or neuroinflammation, producing the characteristic behavioral and neuropathlogical changes arising from injured dopaminergic neurons and glial cells. A number of studies have shown that glia-targeting antioxidants play important roles in protecting against the neurotoxicity caused by psychostimulants or neurotoxins. Phytochemicals, which are non-nutritive plant chemicals, protect dopaminergic neurons and glial cells from damage caused by psychostimulants or neurotoxins. The objective of this review was to evaluate the involvement of glial cells in dopaminergic neuron specific toxicity and to explore the neuroprotective activity of phytochemicals in terms of anti-inflammatory and antioxidant action.

    DOI: 10.1254/jphs.13R19CP

    Web of Science

    researchmap

  • Effects of Bupropion and Pramipexole on Cell Proliferation in the Hippocampus of Adrenocorticotropic Hormone-Treated Rats Reviewed

    Yuka Onoue, Keiko Kuwatsuka, Ikuko Miyazaki, Masato Asanuma, Yoshihisa Kitamura, Toshiaki Sendo

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   37 ( 2 )   327 - 330   2014.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PHARMACEUTICAL SOC JAPAN  

    The dopamine reuptake inhibitor bupropion and dopamine D2/3 receptor agonist pramipexole have been clinically proven to improve both depression and treatment-resistant depression. We examined its influence on the duration of immobility during the forced swim test in adrenocorticotropic hormone (ACTH)-treated rats and further analyzed the possible role of the dopamine nerve system in this effect. Bupropion and pramipexole significantly decreased the duration of immobility in normal and ACTH-treated rats. We previously demonstrated that the chronic administration of ACTH caused a significant decrease in hippocampal cell proliferation and neurogenesis. In this study, we used the mitotic marker 5-bromo-2'-deoxyridine to investigate the effects of bupropion and pramipexole on cell proliferation in the subgranular zone of the hippocampal dentate gyms following chronic treatment with ACTH. The ACTH treatment for 14 d decreased adult hippocampal cell proliferation. The chronic administration of bupropion for 14 d blocked the loss of cell proliferation resulting from the chronic treatment with ACTH, whereas pramipexole did not. The administration of bupropion may have treatment-resistant antidepressive properties, which may be partly attributed to the normalization of hippocampal cell proliferation.

    DOI: 10.1248/bpb.b13-00749

    Web of Science

    researchmap

  • Metallothionein deficiency exacerbates diabetic nephropathy in streptozotocin-induced diabetic mice Reviewed

    Hiromi Tachibana, Daisuke Ogawa, Norio Sogawa, Masato Asanuma, Ikuko Miyazaki, Naoto Terami, Takashi Hatanaka, Chikage Sato Horiguchi, Atsuko Nakatsuka, Jun Eguchi, Jun Wada, Hiroshi Yamada, Kohji Takei, Hirofumi Makino

    AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY   306 ( 1 )   F105 - F115   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER PHYSIOLOGICAL SOC  

    Oxidative stress and inflammation play important roles in diabetic complications, including diabetic nephropathy. Metallothionein (MT) is induced in proximal tubular epithelial cells as an antioxidant in the diabetic kidney; however, the role of MT in renal function remains unclear. We therefore investigated whether MT deficiency accelerates diabetic nephropathy through oxidative stress and inflammation. Diabetes was induced by streptozotocin injection in MT-deficient (MT-/-) and MT+/+ mice. Urinary albumin excretion, histological changes, markers for reactive oxygen species (ROS), and kidney inflammation were measured. Murine proximal tubular epithelial (mProx24) cells were used to further elucidate the role of MT under high-glucose conditions. Parameters of diabetic nephropathy and markers of ROS and inflammation were accelerated in diabetic MT-/- mice compared with diabetic MT+/+ mice, despite equivalent levels of hyperglycemia. MT deficiency accelerated interstitial fibrosis and macrophage infiltration into the interstitium in the diabetic kidney. Electron microscopy revealed abnormal mitochondrial morphology in proximal tubular epithelial cells in diabetic MT-/- mice. In vitro studies demonstrated that knockdown of MT by small interfering RNA enhanced mitochondrial ROS generation and inflammation-related gene expression in mProx24 cells cultured under high-glucose conditions. The results of this study suggest that MT may play a key role in protecting the kidney against high glucose-induced ROS and subsequent inflammation in diabetic nephropathy.

    DOI: 10.1152/ajprenal.00034.2013

    Web of Science

    researchmap

  • Transplantation of Melanocytes Obtained from the Skin Ameliorates Apomorphine-Induced Abnormal Behavior in Rodent Hemi-Parkinsonian Models Reviewed

    Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Youichirou Higashi, Masayoshi Namba, Norio Ogawa

    PLoS ONE   8 ( 6 )   e65983   2013.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Tyrosinase, which catalyzes both the hydroxylation of tyrosine and consequent oxidation of L-DOPA to form melanin in melanocytes, is also expressed in the brain, and oxidizes L-DOPA and dopamine. Replacement of dopamine synthesis by tyrosinase was reported in tyrosine hydroxylase null mice. To examine the potential benefits of autograft cell transplantation for patients with Parkinson's disease, tyrosinase-producing cells including melanocytes, were transplanted into the striatum of hemi-parkinsonian model rats or mice lesioned with 6-hydroxydopamine. Marked improvement in apomorphine-induced rotation was noted at day 40 after intrastriatal melanoma cell transplantation. Transplantation of tyrosinase cDNA-transfected hepatoma cells, which constitutively produce L-DOPA, resulted in marked amelioration of the asymmetric apomorphine-induced rotation in hemi-parkinsonian mice and the effect was present up to 2 months. Moreover, parkinsonian mice transplanted with melanocytes from the back skin of black newborn mice, but not from albino mice, showed marked improvement in the apomorphine-induced rotation behavior up to 3 months after the transplantation. Dopamine-positive signals were seen around the surviving transplants in these experiments. Taken together with previous studies showing dopamine synthesis and metabolism by tyrosinase, these results highlight therapeutic potential of intrastriatal autograft cell transplantation of melanocytes in patients with Parkinson's disease. © 2013 Asanuma et al.

    DOI: 10.1371/journal.pone.0065983

    Scopus

    PubMed

    researchmap

  • Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothionein expression Reviewed

    Norio Sogawa, Kanji Hirai, Chiharu Sogawa, Kazumi Ohyama, Ikuko Miyazaki, Goichi Tsukamoto, Masato Asanuma, Akira Sasaki, Shigeo Kitayama

    Life Sciences   92 ( 12 )   727 - 732   2013.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Aims Cisplatin (CDDP) is a potent anticancer agent, but severe renal toxicity can limit its use. We investigated the protective effect of cepharanthin (CE), a biscoclaurin alkaloid, on the renal toxicity of CDDP. Main methods Mice were given CDDP along with CE. Effects of CE on CDDP toxicity were investigated by assaying markers of renal toxicity together with MT expression, and by histopathological examination of the kidney. MT-null mice were also examined. Key findings CE induced expression of metallothionein (MT). Pre-administration of CE attenuated an increase in blood urea nitrogen (BUN) concentrations after the CDDP injection. A histochemical analysis demonstrated protection against CDDP-induced necrocytosis of kidney tissues by CE. The protective effect of CE did not occur in the MT-null mice. Significance Pretreatment with CE may reduce the renal toxicity of CDDP through expression of MT. © 2013 Elsevier Inc.

    DOI: 10.1016/j.lfs.2013.01.031

    Scopus

    PubMed

    researchmap

  • アストロサイトとParkinson病治療

    浅沼幹人, 宮崎育子

    神経内科   79 ( 2 )   262 - 268   2013

     More details

  • 培養アストログリア細胞における細胞保護効果.

    喜多大三, 浅沼幹人, 宮崎育子, 竹島美香

    九州栄養福祉大学研究紀要   ( 10 )   179 - 191   2013

     More details

  • The mechanisms of electroconvulsive stimuli in BrdU-positive cells of the dentate gyrus in ACTH-treated rats Reviewed

    Keiko Kuwatsuka, Hiromi Hayashi, Yuka Onoue, Ikuko Miyazaki, Toshihiro Koyama, Masato Asanuma, Yoshihisa Kitamura, Toshiaki Sendo

    Journal of Pharmacological Sciences   122 ( 1 )   34 - 41   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    In clinical studies, electroconvulsive stimuli have been associated with improvements in both depression and treatment-resistant depression. In a previous study, treatment with adrenocorticotropic hormone (ACTH) for 14 days decreased adult hippocampal cell proliferation. Furthermore, electroconvulsive stimuli significantly decreased the duration of immobility following repeated administration of ACTH for 14 days in rats. The present study was undertaken to further characterize the mechanism of treatment-resistant antidepressant effects of electroconvulsive stimuli by measuring cell proliferation, brain-derived neurotrophic factor (BDNF) levels, and phosphorylated and total cyclic adenosine monophosphate (cAMP) response element-binding protein (pCREB/CREB) levels in the hippocampus of ACTH-treated rats. Electroconvulsive stimuli increased cell proliferation in both saline-treated and ACTH-treated rats. Mature-BDNF protein levels showed a tendency to decrease in ACTH-treated rats. Electroconvulsive stimuli treatment increased mature-BDNF protein levels in the hippocampus of both saline-treated and ACTH-treated rats. Furthermore, electroconvulsive stimuli increased phospho-Ser133-CREB (pCREB) levels and the ratio of pCREB/CREB in both saline-treated and ACTH-treated rats. These findings suggest that the treatment-resistant antidepressant effects of electroconvulsive stimuli may be attributed, at least in part, to an enhancement of hippocampal cell proliferation. © The Japanese Pharmacological Society.

    DOI: 10.1254/jphs.13015FP

    Scopus

    PubMed

    researchmap

  • Centrosomal aggregates and Golgi fragmentation disrupt vesicular trafficking of DAT Reviewed

    Francisco J. Diaz-Corrales, Ikuko Miyazaki, Masato Asanuma, Diego Ruano, Rosa M. Rios

    Neurobiology of Aging   33 ( 10 )   2462 - 2477   2012.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.neurobiolaging.2011.11.014

    Web of Science

    researchmap

  • Cyclooxygenase-Independent Neuroprotective Effects of Aspirin Against Dopamine Quinone-Induced Neurotoxicity Reviewed

    Masato Asanuma, Ikuko Miyazaki, Yuri Kikkawa, Naotaka Kimoto, Mika Takeshima, Shinki Murakami, Ko Miyoshi

    NEUROCHEMICAL RESEARCH   37 ( 9 )   1944 - 1951   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER/PLENUM PUBLISHERS  

    Prostaglandin H synthase exerts not only cyclooxygenase activity but also peroxidase activity. The latter activity of the enzyme is thought to couple with oxidation of dopamine to dopamine quinone. Therefore, it has been proposed that cyclooxygenase inhibitors could suppress dopamine quinone formation. In the present study, we examined effects of various cyclooxygenase inhibitors against excess methyl L-3,4-dihydroxyphenylalanine (L-DOPA)-induced quinoprotein (protein-bound quinone) formation and neurotoxicity using dopaminergic CATH.a cells. The treatment with aspirin inhibited excess methyl L-DOPA-induced quinoprotein formation and cell death. However, acetaminophen did not show protective effects, and indomethacin and meloxicam rather aggravated these methyl L-DOPA-induced changes. Aspirin and indomethacin did not affect the level of glutathione that exerts quenching dopamine quinone in dopaminergic cells. In contrast with inhibiting effects of higher dose in the previous reports, relatively lower dose of aspirin that affected methyl L-DOPA-induced quinoprotein formation and cell death failed to prevent cyclooxygenase-induced dopamine chrome generation in cell-free system. Furthermore, aspirin but not acetaminophen or meloxicam showed direct dopamine quinone-scavenging effects in dopamine-semiquinone generating systems. The present results suggest that cyclooxygenase shows little contribution to dopamine oxidation in dopaminergic cells and that protective effects of aspirin against methyl L-DOPA-induced dopamine quinone neurotoxicity are based on its cyclooxygenase-independent property.

    DOI: 10.1007/s11064-012-0813-2

    Web of Science

    researchmap

  • EFFLUENT FREE RADICALS ARE ASSOCIATED WITH RESIDUAL RENAL FUNCTION AND PREDICT TECHNIQUE FAILURE IN PERITONEAL DIALYSIS PATIENTS Reviewed

    Hiroshi Morinaga, Hitoshi Sugiyama, Tatsuyuki Inoue, Keiichi Takiue, Yoko Kikumoto, Masashi Kitagawa, Shigeru Akagi, Kazushi Nakao, Yohei Maeshima, Ikuko Miyazaki, Masato Asanuma, Makoto Hiramatsu, Hirofumi Makino

    PERITONEAL DIALYSIS INTERNATIONAL   32 ( 4 )   453 - 461   2012.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MULTIMED INC  

    Objective: Residual renal function (RRF) is associated with low oxidative stress in peritoneal dialysis (PD). In the present study, we investigated the relationship between the impact of oxidative stress on RRF and patient outcomes during PD.
    Methods: Levels of free radicals (FRs) in effluent from the overnight dwell in 45 outpatients were determined by electron spin resonance spectrometry. The FR levels, clinical parameters, and the level of 8-hydroxy-2'-deoxyguanosine were evaluated at study start. The effects of effluent FR level on technique and patient survival were analyzed in a prospective cohort followed for 24 months.
    Results: Levels of effluent FRs showed significant negative correlations with daily urine volume and residual renal Kt/V, and positive correlations with plasma beta(2)-microglobulin and effluent 8-hydroxy-2'-deoxyguanosine. A highly significant difference in technique survival (p &lt; 0.05), but not patient survival, was observed for patients grouped by effluent FR quartile. The effluent FR level was independently associated with technique failure after adjusting for patient age, history of cardiovascular disease, and presence of diabetes mellitus (p &lt; 0.001). The level of effluent FRs was associated with death-censored technique failure in both univariate (p &lt; 0.001) and multivariate (p &lt; 0.01) hazard models. Compared with patients remaining on PD, those withdrawn from the modality had significantly higher levels of effluent FRs (p &lt; 0.005).
    Conclusions: Elevated effluent FRs are associated with RRF and technique failure in stable PD patients. These findings highlight the importance of oxidative stress as an unfavorable prognostic factor in PD and emphasize that steps should be taken to minimize oxidative stress in these patients. Perit Dial Int 2012; 32(4): 453-461

    DOI: 10.3747/pdi.2011.00032

    Web of Science

    researchmap

  • ACTH反復投与ラットにおける海馬細胞新生の減少及びそのメカニズムに関する検討.

    林 宏美, 土居真穂, 尾上由華, 鍬塚圭子, 三宅彩香, 小山敏広, 四宮一昭, 宮崎育子, 浅沼幹人, 北村佳久

    薬学雑誌   173 - 178   2012

  • Bibliographic review on the central actions of theanine

    ( 9 )   45 - 58   2012

     More details

  • Protective effects of baicalein against excess L-DOPA-induced dopamine quinone neurotoxicity Reviewed

    Mika Takeshima, Maiko Murata, Natsuho Urasoe, Shinki Murakami, Ikuko Miyazaki, Masato Asanuma, Taizo Kita

    NEUROLOGICAL RESEARCH   33 ( 10 )   1050 - 1056   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MANEY PUBLISHING  

    Objectives: Baicalein, a flavonoid derived from the root of Scutelaria baicalensis Georgi, possesses antioxidative properties including reactive oxygen species scavenging and lipid peroxidation inhibiting activities. The present study was undertaken to investigate the neuroprotective effect of baicalein against dopamine (DA) neurotoxicity induced by exposure to a synthetic DA precursor, L-3,4-dihydroxyphenylalanine (L-DOPA), in cultured dopaminergic CATH. a cells.
    Methods and results: Exposure to L-DOPA for 24 hours reduced the number of viable cells and enhanced protein-bound quinone (quinoprotein) formation in the cell. Both effects were prevented by simultaneous treatment with baicalein. In addition, baicalein prevented the formation of DA semiquinone radicals from DA in an in vitro cell-free system. Long-term baicalein treatment for 96 hours also protected against excess L-DOPA-induced cell death, and also increased glutathione (GSH) levels in CATH. a cells.
    Discussion: Our results indicate that baicalein has neuroprotective properties against excess L-DOPA-induced DA neurotoxicity through the suppression of DA quinone formation. Furthermore, the long-term treatment of baicalein upregulates intracellular GSH contents, which may also exert neuroprotective effects against oxidative stress-induced neuronal damage.

    DOI: 10.1179/1743132811Y.0000000032

    Web of Science

    researchmap

  • Factors That Influence Primary Cilium Length Reviewed

    Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Masato Asanuma

    ACTA MEDICA OKAYAMA   65 ( 5 )   279 - 285   2011.10

     More details

    Language:English   Publisher:OKAYAMA UNIV MED SCHOOL  

    Almost all mammalian cells carry one primary cilium that functions as a biosensor for chemical and mechanical stimuli. Genetic damages that compromise cilia formation or function cause a spectrum of disorders referred to as ciliapathies. Recent studies have demonstrated that some pharmacological agents and extracellular environmental changes can alter primary cilium length. Renal injury is a well-known example of an environmental insult that triggers cilia length modification. Lithium treatment causes primary cilia to extend in several cell types including neuronal cells; this phenomenon is likely independent of glycogen synthase kinase-3 beta inhibition. In renal epithelial cell lines, deflection of the primary cilia by fluid shear shortens them by reducing the intracellular cyclic AMP level, leading to a subsequent decrease in mechanosensitivity to fluid shear. Primary cilium length is also influenced by the dynamics of actin filaments and microtubules through the levels of soluble tubulin in the cytosol available for primary cilia extension. Thus, mammalian cells can adapt to the extracellular environment by modulating the primary cilium length, and this feedback system utilizing primary cilia might exist throughout the mammalian body. Further investigation is required concerning the precise molecular mechanisms underlying the control of primary cilium length in response to environmental factors.

    DOI: 10.18926/AMO/47009

    Web of Science

    researchmap

  • A Novel Hypothesized Clinical Implication of Zonisamide for Autism Reply

    Masato Asanuma, Ikuko Miyazaki

    ANNALS OF NEUROLOGY   69 ( 2 )   426 - 427   2011.2

     More details

    Language:English   Publisher:WILEY-BLACKWELL  

    DOI: 10.1002/ana.22347

    Web of Science

    researchmap

  • Chronic Treatment with Imipramine and Lithium Increases Cell Proliferation in the Hippocampus in Adrenocorticotropic Hormone-Treated Rats Reviewed

    Yoshihisa Kitamura, Maho Doi, Keiko Kuwatsuka, Yuka Onoue, Ikuko Miyazaki, Kazuaki Shinomiya, Toshihiro Koyama, Toshiaki Sendo, Hiromu Kawasaki, Masato Asanuma, Yutaka Gomita

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   34 ( 1 )   77 - 81   2011.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PHARMACEUTICAL SOC JAPAN  

    Adult hippocampal neurogenesis is reported to change in animal models of depression and antidepressants. We have used the mitotic marker 5-bromo-2'-deoxyyridine to address the effects of imipramine and lithium on cell proliferation and survival following chronic treatment with adrenocorticotropic hormone (ACTH) in the subgranular zone of the hippocampal dentate gyrus. ACTH treatment for 14d decreased adult hippocampal cell proliferation and survival. Coadministration of imipramine and lithium for 14d blocked the loss of cell proliferation but not cell survival resulting from the chronic treatment with ACTH. The coadministration of imipramine and lithium may have treatment-resistant antidepressive properties, which may be attributed, in part, to a normalization of hippocampal cell proliferation.

    DOI: 10.1248/bpb.34.77

    Web of Science

    researchmap

  • パーキンソン病とアストロサイト─新たな神経保護療法の標的

    浅沼幹人, 宮崎育子

    Clinical Neuroscience   29   1295 - 1297   2011

     More details

  • A study on L-theanine-mediated cytoprotection in glial cells : L-theanine up-regulates antioxidant defenses in astrocytes

    8 ( 8 )   43 - 53   2011

     More details

  • High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells Reviewed International journal

    Daisuke Ogawa, Masato Asanuma, Ikuko Miyazaki, Hiromi Tachibana, Jun Wada, Norio Sogawa, Takeshi Sugaya, Shinji Kitamura, Yohei Maeshima, Kenichi Shikata, Hirofumi Makino

    Experimental Diabetes Research   2011   1 - 8   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Hindawi Limited  

    Metallothionein (MT) is an intracellular metal-binding, cysteine-rich protein, and is a potent antioxidant that protects cells and tissues from oxidative stress. Although the major isoforms MT-1 and -2 (MT-1/-2) are highly inducible in many tissues, the distribution and role of MT-1/-2 in diabetic nephropathy are poorly understood. In this study, diabetes was induced in adult male rats by streptozotocin, and renal tissues were stained with antibodies for MT-1/-2. MT-1/-2 expression was also evaluated in mProx24 cells, a mouse renal proximal tubular epithelial cell line, stimulated with high glucose medium and pretreated with the antioxidant vitamin E. MT-1/-2 expression was gradually and dramatically increased, mainly in the proximal tubular epithelial cells and to a lesser extent in the podocytes in diabetic rats, but was hardly observed in control rats. MT-1/-2 expression was also increased by high glucose stimulation in mProx24 cells. Because the induction of MT was suppressed by pretreatment with vitamin E, the expression of MT-1/-2 is induced, at least in part, by high glucose-induced oxidative stress. These observations suggest that MT-1/-2 is induced in renal proximal tubular epithelial cells as an antioxidant to protect the kidney from oxidative stress, and may offer a novel therapeutic target against diabetic nephropathy.

    DOI: 10.1155/2011/534872

    Web of Science

    PubMed

    researchmap

    Other Link: http://downloads.hindawi.com/journals/jdr/2011/534872.xml

  • Effects of Imipramine and Lithium on the Suppression of Cell Proliferation in the Dentate Gyrus of the Hippocampus in Adrenocorticotropic Hormone-treated Rats Reviewed

    Maho Doi, Ikuko Miyazaki, Tomoko Nagamachi, Kazuaki Shinomiya, Hisashi Matsunaga, Toshiaki Sendo, Hiromu Kawasaki, Masato Asanuma, Yutaka Gomita, Yoshihisa Kitamura

    ACTA MEDICA OKAYAMA   64 ( 4 )   219 - 223   2010.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We examined the influence of chronic adrenocorticotropic hormone (ACTH) treatment on the number of Ki-67-positive cells in the dentate gyrus of the hippocampus in rats. ACTH treatment for 14 days decreased the number of such cells. The administration of imipramine or lithium alone for 14 days had no effect in saline-treated rats. The effect of ACTH was blocked by the administration of imipramine. Furthermore, the coadministration of imipramine and lithium for 14 days significantly increased the number of Ki-67-positive cells in both the saline and ACTH-treated rats. The coadministration of imipramine and lithium normalized the cell proliferation in the dentate gyrus of the hippocampus in rats treated with ACTH.

    Web of Science

    researchmap

  • Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice Reviewed

    Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Shoko Shimizu, Manabu Taniguchi, Shinsuke Matsuzaki, Masaya Tohyama, Masato Asanuma

    FASEB JOURNAL   23 ( 10 )   3289 - 3297   2009.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FEDERATION AMER SOC EXP BIOL  

    The Drosophila pericentrin-like protein has been shown to be essential for the formation of the sensory cilia of chemosensory and mechanosensory neurons by mutant analysis in flies, while the in vivo function of pericentrin, a well-studied mammalian centrosomal protein related to microcephalic primordial dwarfism, has been unclear. To determine whether pericentrin is required for ciliogenesis in mammals, we generated and analyzed mice with a hypomorphic mutation of Pcnt encoding the mouse pericentrin. Immunofluorescence analysis demonstrated that olfactory cilia of chemosensory neurons in the nasal olfactory epithelium were malformed in the homozygous mutant mice. On the other hand, the assembly of motile and primary cilia of non-neuronal epithelial cells and the formation of sperm flagella were not affected in the Pcnt-mutant mice. The defective assembly of olfactory cilia in the mutant was apparent from birth. The mutant animals displayed reduced olfactory performance in agreement with the compromised assembly of olfactory cilia. Our findings suggest that pericentrin is essential for the assembly of chemosensory cilia of olfactory receptor neurons, but it is not globally required for cilia formation in mammals.-Miyoshi, K., Kasahara, K., Miyazaki, I., Shimizu, S., Taniguchi, M., Matsuzaki, S., Tohyama, M., Asanuma, M. Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice. FASEB J. 23, 3289-3297 ( 2009). www.fasebj.org

    DOI: 10.1096/fj.08-124420

    Web of Science

    researchmap

  • Lithium treatment elongates primary cilia in the mouse brain and in cultured cells Reviewed

    Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Masato Asanuma

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   388 ( 4 )   757 - 762   2009.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The molecular mechanisms underlying the therapeutic effects of lithium, a first-line antimanic mood stabilizer, have not yet been fully elucidated. Treatment of the algae Chlamydomonas reinhardtii with lithium has been shown to induce elongation of their flagella, which are analogous structures to vertebrate cilia. In the mouse brain, adenylyl cyclase 3 (AC3) and certain neuropeptide receptors colocalize to the primary cilium of neuronal cells, suggesting a chemosensory function for the primary cilium in the nervous system. Here we show that lithium treatment elongates primary cilia in the mouse brain and in cultured cells. Brain sections from mice chronically fed with Li(2)CO(3) were subjected to immunofluorescence study. Primary cilia carrying both AC3 and the receptor for melanin-concentrating hormone (MCH) were elongated in the dorsal striatum and nucleus accumbens of lithium-fed mice, as compared to those of control animals. Moreover, lithium-treated NIH3T3 cells and cultured striatal neurons exhibited elongation of the primary cilia. The present results provide initial evidence that a psychotropic agent can affect ciliary length in the central nervous system, and furthermore suggest that lithium exerts its therapeutic effects via the upregulation of cilia-mediated MCH sensing. These findings thus contribute novel insights into the pathophysiology of bipolar mood disorder and other psychiatric diseases. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2009.08.099

    Web of Science

    researchmap

  • Reduction of Nuclear Peroxisome Proliferator-Activated Receptor gamma Expression in Methamphetamine-Induced Neurotoxicity and Neuroprotective Effects of Ibuprofen Reviewed

    Takeshi Tsuji, Masato Asanuma, Ikuko Miyazaki, Ko Miyoshi, Norio Ogawa

    NEUROCHEMICAL RESEARCH   34 ( 4 )   764 - 774   2009.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER/PLENUM PUBLISHERS  

    We examined changes in nuclear peroxisome proliferator-activated receptor gamma (PPAR gamma) in the striatum in methamphetamine (METH)-induced dopaminergic neurotoxicity, and also examined effects of treatment with drugs possessing PPAR gamma agonistic properties. The marked reduction of nuclear PPAR gamma-expressed cells was seen in the striatum 3 days after METH injections (4 mg/kg x 4, i.p. with 2-h interval). The reduction of dopamine transporter (DAT)-positive signals and PPAR gamma expression, and accumulation of activated microglial cells were significantly and dose-dependently attenuated by four injections of a nonsteroidal anti-inflammatory drug and a PPAR gamma ligand, ibuprofen (10 or 20 mg/kg x 4, s.c.) given 30 min prior to each METH injection, but not by either a low or high dose of aspirin. Either treatment of ibuprofen or aspirin, that showed no effects on METH-induced hyperthermia, significantly blocked the METH-induced striatal cyclooxygenase (COX) expression. Furthermore, the treatment of an intrinsic PPAR gamma ligand 15d-PG J2 also attenuated METH injections-induced reduction of striatal DAT. Therefore, the present study suggests the involvement of reduction of PPAR gamma expression in METH-induced neurotoxicity. Taken together with the previous report showing protective effects of other PPAR gamma ligand, these results imply that the protective effects of ibuprofen against METH-induced neurotoxicity may be based, in part, on its anti-inflammatory PPAR gamma agonistic properties, but not on its COX-inhibiting property or hypothermic effect.

    DOI: 10.1007/s11064-008-9863-x

    Web of Science

    researchmap

  • 食材および食品に含まれる化学物質の生理活性とその機能―ドパミン神経培養系におけるフィチン酸の作用―

    浦添 夏帆, 村田 麻衣子, 青柳 東彦, 安東 勢津子, 宮崎 育子, 浅沼 幹人, 喜多 大三

    九州栄養福祉大学研究紀要   6   75 - 86   2009

     More details

  • DOPAMINE-INDUCED BEHAVIORAL CHANGES AND OXIDATIVE STRESS IN METHAMPHETAMINE-INDUCED NEUROTOXICITY Reviewed

    Taizo Kita, Ikuko Miyazaki, Masato Asanuma, Mika Takeshima, George C. Wagner

    NEW CONCEPTS OF PSYCHOSTIMULANTS INDUCED NEUROTOXICITY   88   43 - 64   2009

     More details

    Language:English   Publishing type:Part of collection (book)   Publisher:ELSEVIER ACADEMIC PRESS INC  

    DOI: 10.1016/S0074-7742(09)88003-3

    Web of Science

    researchmap

  • Dopamine Induces Supernumerary Centrosomes and Subsequent Cell Death through Cdk2 up-Regulation in Dopaminergic Neuronal Cells Reviewed

    Francisco J. Diaz-Corrales, Masato Asanuma, Ikuko Miyazaki, Ko Miyoshi, Nobutaka Hattori, Norio Ogawa

    NEUROTOXICITY RESEARCH   14 ( 4 )   295 - 305   2008.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Aggregation of proteins in the centrosome is implicated in the pathophysiology of Parkinson&apos;s disease. However, the relevance of the centrosome in neurodegeneration is still obscure. Centrosome duplication is initiated by the cyclin E/cyclin-dependent kinase 2 (Cdk2) complex. The present study determined changes in cyclin E or Cdk2 expression and in the centrosomal structure in dopaminergic neuronal CATH.a cells exposed to 50, 100 and 150 mu M dopamine (DA) for 24 h. DA induced significant increase in Cdk2 protein and cyclin E protein, but not cyclin a mRNA. In DA-treated cells, the intense cyclin E- and Cdk2-immunofluorescence signals were co-localized around large and supernumerary centrosomes, and these two parameters of centrosome amplification were significantly increased compared with the control. Simultaneous co-treatment with DA and a Cdk2 inhibitor blocked centrosome amplification and enhanced cell viability. Our results demonstrated that DA could lead to cyclin E accumulation and Cdk2 up-regulation triggering supernumerary centrosomes and apoptotic cell death.

    DOI: 10.1007/BF03033854

    Web of Science

    researchmap

  • Protective effects of interferon-gamma against methamphetamine-induced neurotoxicity (vol 177, pg 123, 2008) Reviewed

    Hozumi Hiroaki, Asanuma Masato, Miyazaki Ikuko, Fukuoka Saki, Kikkawa Yuri, Kimoto Naotaka, Kitamura Yoshihisa, Sendo Toshiaki, Kita Taizo, Gomita Yutaka

    TOXICOLOGY LETTERS   180 ( 3 )   231   2008.8

  • Effects of HMGB1 on ischemia-reperfusion injury in the rat heart Reviewed

    Susumu Oozawa, Shuji Mori, Toru Kanke, Hideo Takahashi, Keyue Liu, Yasuko Tomono, Masato Asanuma, Ikuko Miyazaki, Masahiro Nishibori, Shunji Sano

    CIRCULATION JOURNAL   72 ( 7 )   1178 - 1184   2008.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE CIRCULATION SOC  

    Background Coronary ischemia-reperfusion (I/R) injury causes cardiomyocyte necrosis in a multi-step process that includes an inflammatory reaction. A recent study has suggested that high-mobility group box 1 (HMGB 1) is a late mediator of lethal sepsis and an early mediator of inflammation and necrosis following I/R injury. In the present study a neutralizing monoclonal antibody (mAb) for HMGB1 was used to clarify the role of HMGB 1 in cardiac I/R injury.
    Methods and Results Rats underwent 30 min of left coronary artery occlusion followed by 60 min reperfusion. An intravenous injection of anti-HMGB1 mAb or control IgG was administered just before reperfusion. The infarct size was enlarged in the anti-HMGB1 mAb group in comparison with the control group (p&lt;0.05). The treatment of anti-HMGB1 mAb significantly increased the plasma troponin-T and norepinephrine (NE) content in the heart in comparison with the control (p&lt;0.05). Moreover, the production of dihydroxyphenylglycol was reduced in the anti-HMGB1-treated group (p&lt;0.05).
    Conclusion This study shows for the first time the effects of treatment with neutralizing anti-HMGB1 mAb on I/R injury in the rat heart. The findings support the novel view that I/R-induced HMGB1 may be an important factor in the modulation of interstitial NE.

    DOI: 10.1253/circj.72.1178

    Web of Science

    researchmap

  • Nonsteroidal anti-inflammatory drugs in experimental parkinsonian models and Parkinson's disease Reviewed

    Masato Asanuma, Ikuko Miyazaki

    CURRENT PHARMACEUTICAL DESIGN   14 ( 14 )   1428 - 1434   2008.5

     More details

    Language:English   Publisher:BENTHAM SCIENCE PUBL LTD  

    A number of experimental studies using parkinsonian models have revealed that nonsteroidal anti-inflammatory drugs (NSAIDs) have neuroprotective properties against dopaminergic neurotoxicity not only by their cyclooxygenase-inhibiting effect but also by other specific properties or some unknown pharmacological effects. This article reviews heterogeneous pharmacological properties of NSAIDs including inhibitory effect against nitric oxide synthesis, agonistic action for peroxisome proliferator-activated receptor or possible suppressive effects against dopamine quinone generation, and also reviews their neuroprotective effects in the experimental parkinsonian models and pathogenesis of Parkinson's disease. Several epidemiological studies recently clarified that the use of nonaspirin NSAIDs but not aspirin was associated with a lower prevalence of Parkinson's disease, in contrast with neuroprotective effects of aspirin in the experimental studies. It also discusses the discrepancy between results in the experimental parkinsonian models and epidemiological data in prevalence of Parkinson's disease on the effects of NSAIDs.

    DOI: 10.2174/138161208784480153

    Web of Science

    researchmap

  • Specific induction of PAG608 in cranial and spinal motor neurons of L-DOPA-treated parkinsonian rats Reviewed

    Masako Shimizu, Ikuko Miyazaki, Youichirou Higashi, Maria J. Eslava-Alva, Francisco J. Diaz-Coffales, Masato Asanuma, Norio Ogawa

    NEUROSCIENCE RESEARCH   60 ( 4 )   355 - 363   2008.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    We identified p53-activated gene 608 (PAG608) as a specifically induced gene in striatal tissue of L-DOPA (100 mg/kg)-injected hemi-parkinsonian rats using differential display assay. In the present study, we further examined morphological distribution of PAG608 in the central nervous system of L-DOPA-treated hemi-parkinsonian rats. PAG608 expression was markedly induced in fibers and neuronal cells of the lateral globus pallidus and reticular thalamic nucleus adjacent to internal capsule, specifically in the parkinsonian side of L-DOPA-treated models. The protein was also constitutively expressed in motor neurons specifically in either side of the pontine nucleus and motor nuclei of trigeminal and facial nerves. Furthermore, L-DOPA-induced PAG608 expression on motor neurons in the contralateral side of the ventral horn of the spinal cord and the lateral corticospinal tract without cell loss. The specific induction of PAG608 6-48 h after L-DOPA injection in the extrapyramidal tracts, pyramidal tracts and corresponding lower motor neurons of the spinal cords suggests its involvement in molecular events in stimulated motor neurons. Taken together with the constitutive expression of PAG608 in the motor nuclei of cranial nerves, PAG608 may be a useful marker of stressed or activated lower motor neurons. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2007.12.006

    Web of Science

    researchmap

  • Protective effects of interferon-gamma against methamphetamine-induced neurotoxicity Reviewed

    Hiroaki Hozumi, Masato Asanuma, Ikuko Miyazaki, Saki Fukuoka, Yuri Kikkawa, Naotaka Kimoto, Yoshihisa Kitamura, Toshiaki Sendo, Taizo Kita, Yutaka Gomita

    TOXICOLOGY LETTERS   177 ( 2 )   123 - 129   2008.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    Repeated injections of methamphetamine (METH) cause degeneration of striatal dopaminergic nerve terminals. In the present study, we examined the effects of interferon-gamma (IFN-gamma) on METH-induced striatal neurotoxicity in mice. Intraperitoneal injection of IFN-gamma before METH injection significantly prevented METH-induced reduction of striatal dopamine transporter (DAT)-positive signals and hyperthermia. Furthermore, intracerebroventricular injection of IFN-gamma before METH treatment markedly prevented METH-induced reduction of DAT. Interestingly, central IFN-gamma injection had no effect on METH-induced hyperthermia. In addition, IFN-gamma injected centrally after METH treatment, but not systemically, 1 h after the final METH injection significantly protected against METH-induced neurotoxicity. Our results suggest that IFN-gamma injected systemically or its related molecule protects against METH-induced neurotoxicity through intracerebral molecular pathways, while it can prevent METH-induced hyperthermia through different molecular events. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.toxlet.2008.01.005

    Web of Science

    researchmap

  • Biological roles for neuronal primary cilia Reviewed

    Ko Miyoshi, Ikuko Miyazaki, Masato Asanuma

    PSYCHIATRY AND CLINICAL NEUROSCIENCES   62 ( 1 )   S6 - S6   2008.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL PUBLISHING  

    Web of Science

    researchmap

  • Preventing effects of a novel anti-parkinsonian agent zonisamide on dopamine quinone formation Reviewed

    Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Ko Miyoshi, Norio Ogawa, Miho Murata

    NEUROSCIENCE RESEARCH   60 ( 1 )   106 - 113   2008.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    The neurotoxicity of dopamine (DA) quinones as dopaminergic neuron-specific oxidative stress is considered to play a role in the pathogenesis and/or progression of Parkinson's disease (PD), since DA quinones conjugate with several key PD pathogenic molecules (e.g., tyrosine hydroxylase, alpha-synuclein and parkin) to form protein-bound quinone (quinoprotein) and consequently inhibit their functions. Zonisamide (ZNS) is used as an anti-epileptic agent but also improved the cardinal symptoms of PD in recent clinical trials in Japan. To evaluate the effects of ZNS on excess cytosolic free DA-induced quinone toxicity, we examined changes in DA quinone-related indices after ZNS treatment both in in vitro cell-free system and in cultured cells. Co-incubation of DA and ZNS in a cell-free system caused conversion of DA to stable melanin via formation of DA-semiquinone radicals and DA chrome. Long-term (5 days) treatment with ZNS decreased quinoprotein and increased DA/DOPA chromes in dopaminergic CATH.a cells. ZNS significantly inhibited quinoprotein formation induced by treatment with tetrahydrobiopterin and ketanserin that elevate cytosolic free DA in the cells. Our results suggest that the novel anti-parkinsonian agent ZNS possesses preventing effects against DA quinone formation induced by excess amount of cytosolic DA outside the synaptic vesicles. (c) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2007.10.002

    Web of Science

    researchmap

  • A study on the effect of chemicals migrated from food and its ingredient in cultured monoamine neuronal cells: effect of docosahexaenoic acid on dopamine-induced neurotoxicity

    Bulletin of Kyushu Nutrition Welfare University   5 ( 5 )   55 - 67   2008

     More details

  • Effects of L-theanine on cultured dopaminergic neuronal and glial cells

    Bulletin of Kyushu Nutrition Welfare University   5 ( 5 )   69 - 81   2008

     More details

  • メタンフェタミン神経毒性発現に関する研究の新展開

    喜多大三, 竹島美香, Wagner GC, 穂積宏彰, 宮崎育子, 浅沼幹人

    日本神経精神薬理学会雑誌   28   49 - 61   2008

     More details

  • Quinone formation as a common neurotoxic factor in dopaminergic neurotoxicity induced by an excess amount of cytosolic dopamine

    Miyazaki Ikuko, Asanuma Masato, DIAZ-CORRALES Francisco J., Miyoshi Ko, Ogawa Norio

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   119 ( 3 )   235 - 239   2008

     More details

    Language:Japanese   Publisher:Okayama Medical Association  

    DOI: 10.4044/joma.119.235

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://ousar.lib.okayama-u.ac.jp/13216

  • Quinone formation as a common factor for dopamine neuron damages by excessive dopamine outside of vesicles

    Ikuko Miyazaki, Masato Asanuma, Francisco J. Diaz-Corrales, Ko Miyoshi, Norio Ogawa

    Journal of Okayama Medical Association   119 ( 3 )   235 - 239   2008

     More details

  • Common anti-inflammatory drugs are potentially therapeutic for Parkinson's disease? Reviewed

    Masato Asanuma, Ikuko Miyazaki

    EXPERIMENTAL NEUROLOGY   206 ( 2 )   172 - 178   2007.8

     More details

    Language:English   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    DOI: 10.1016/j.expneurol.2007.05.006

    Web of Science

    researchmap

  • Suppression of p53-activated gene, PAG608, attenuates methamphetamine-induced neurotoxicity Reviewed

    Masato Asanuma, Ikuko Miyazaki, Youichirou Higashi, Francisco J. Diaz-Corrales, Masako Shimizu, Ko Miyoshi, Norio Ogawa

    NEUROSCIENCE LETTERS   414 ( 3 )   263 - 267   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    The p53-activated gene 608 (PAG608) is a proapoptotic gene activated and regulated by p53 expression in oxidative stress-induced apoptosis of neuronal cells. In this study, we determined the role of PAG608 in methamphetamine-induced neurotoxicity. Treatment of mouse dopaminergic CATH.a cells with 2 mM methamphetamine increased PAG608 expression at 3 h followed by increase in phosphorylated p53 expression. Transient transfection of PAG608 antisense cDNA or RNA interference using PAG608 small interfering RNA significantly attenuated the dose-dependent decrease in cell viability of CATH.a cells by methamphetamine (1-4 mM) exposure. In monoaminergic neuronal B65 cells, which contain serotonin rather than dopamine, methamphetamine-induced cell death was also significantly but partially protected by transient transfection of PAG608 antisense cDNA. Furthermore, cell death of PC 12 cells produced by methamphetamine (1-5 mM) was almost completely prevented by stable expression of PAG608 antisense cDNA, compared with significant reduction of cell viability in control PC12 cells. Our results showed that suppression of PAG608 using transient and stable transfection with PAG608 anti sense cDNA or small interfering RNA attenuates methamphetamine-induced death of various monoaminergic neuronal cells, suggesting that methamphetamine neurotoxicity in monoaminergic cells is related, at least in part, to induction of PAG608 expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2006.12.036

    Web of Science

    researchmap

  • A study on dopamine-induced neurotoxicity on chemicals migrated from food container and cans: effect of bisphenol A on cultured dopamine neuronal cells

    Bulletin of Kyushu Nutrition Welfare University   4 ( 4 )   89 - 97   2007

     More details

  • Involvement of STAT3 in bladder smooth muscle hypertrophy following bladder outlet obstruction

    Osamu Fujita, Masato Asanuma, Teruhiko Yokoyama, Ikuko Miyazaki, Norio Ogawa, Hiromi Kumon

    ACTA MEDICA OKAYAMA   60 ( 6 )   299 - 309   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We examined the involvement of the signal transducer and activator of transcription 3 (STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using a rat in vivo and in vitro study. BOO induced increases in bladder weight and bladder smooth muscle thickness I week after the operation. By using antibody microarrays, 64 of 389 proteins blotted on the array met our selection criteria of an INR value between &gt;= 2.0 and &lt;= 0.5. This result revealed up-regulation of transcription factors, cell cycle regulatory proteins, apoptosis-associated proteins and so on. On the other hand, down-regulation (INR value &lt;= 0.5) of proteins was not found. In a profiling study, we found an increase in the expression of STAT3. A significant increase in nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (I Hz) at 120% elongation significantly increased the expression of STAT3 and of alpha-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, the blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented the stretch-induced increase in alpha-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy.

    DOI: 10.18926/AMO/30724

    Web of Science

    researchmap

  • Characterization of pericentrin isoforms in vivo Reviewed

    Ko Miyoshi, Masato Asanuma, Ikuko Miyazaki, Shinsuke Matsuzaki, Masaya Tohyama, Norio Ogawa

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   351 ( 3 )   745 - 749   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Pericentrin was first identified as a mouse centrosomal protein and is now referred to as pericentrin A. A larger homologous protein in humans with a C-terminal calmodulin-binding domain was later identified as pericentrin B. Pericentrin has been shown to be one of the key components in ciliogenesis.. but in vivo pericentrin products have remained ambiguous. Here we characterized pericentrin isoforms in mice. Two pericentrin transcripts of 9.5 and 6.9 kb were recognized on the mouse tissue Northern blots, while a cRNA probe for a 5'-terminal sequence shared by pericentrin A and B failed to hybridize to the 6.9-kb message. Two pericentrin cDNAs were identified, which encoded pericentrin B and a novel isoform, pericentrin S, sharing with pericentrin B a C-terminal calmodulin-binding motif. Three pericentrin proteins of 360, 255, and 250 kDa revealed by immunoprecipitation analysis were thought to correspond to pericentrin B, pericentrin S, and an unknown N-terminal product. (c) 2006 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2006.10.101

    Web of Science

    researchmap

  • Nonsteroidal anti-inflammatory drugs in Parkinson’s disease: possible involvement of quinone formation Reviewed

    Masato Asanuma, Ikuko Miyazaki

    Expert Review of Neurotherapeutics   6 ( 9 )   1313 - 1325   2006.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1586/14737175.6.9.1313

    researchmap

  • Embryonic expression of pericentrin suggests universal roles in ciliogenesis Reviewed

    Ko Miyoshi, Kazunari Onishi, Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Norio Ogawa

    DEVELOPMENT GENES AND EVOLUTION   216 ( 9 )   537 - 542   2006.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Pericentrin (Pcnt) is a giant coiled-coil protein known to mediate microtubule organization. It has been recently reported that mitosis-specific centrosomal anchoring of gamma tubulin complexes by Pcnt acts to control mitotic spindle organization, though little is known about the in vivo expression of Pcnt. In this study, we investigated Pcnt expression in mouse embryos. In situ hybridization analysis revealed preferential expression of Pcnt in quiescent G(0) phase cells throughout the embryo with an unexpectedly low expression level in proliferating cells, suggesting that Pcnt might not play an important role in mitotic proliferation. Immunofluorescence analysis confirmed preferential expression of the Pcnt protein in G(0) phase cells. Moreover, Pcnt was shown to be localized to the base of primary cilia in multiple embryonic tissues, in agreement with a recent study demonstrating the involvement of Pcnt in primary cilia formation using cultured mammalian cells.

    DOI: 10.1007/s00427-006-0065-8

    Web of Science

    researchmap

  • Centrosome overduplication induced by rotenone treatment affects the cellular distribution of p53 tumor suppressor protein in the neuroblastoma B65 cell line Reviewed

    Francisco J. Diaz-Corrales, Masato Asanuma, Ikuko Miyazaki, Ko Miyoshi, Norio Ogawa

    PSYCHIATRY AND CLINICAL NEUROSCIENCES   60   S18 - S26   2006.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL PUBLISHING  

    Recently, the formation of inclusion bodies in several neurodegenerative diseases, including Parkinson's disease, has been associated with the aggregation of unfolded proteins recruited in the centrosome. We have reported previously that rotenone, an insecticide that is used to produce experimental models of Parkinsonism, induced the aggregation of the alpha-synuclein protein in the centrosome, and it notably affected the structure and function of this organelle in primary cultures of mesencephalic neurons and astrocytes. However, it is still obscure the mechanisms through which the disorganization and centrosomal dysfunction could induce cell death. In this study the rat neuroblastoma B65 cell line was chronically exposed to rotenone, and then the distribution of the centrosomal protein gamma-tubulin was studied by immunocytochemistry and Western blot analyses. Finally, the configuration of mitotic spindles and distribution of the p53 protein was observed in the control and rotenone-treated groups. Rotenone treatment increased the number of cells having centrosome overduplication and multipolar mitotic spindles. In contrast, rotenone induced redistribution of the p53 protein, which was colocalized with the gamma-tubulin protein in the perinuclear region of cells having overduplicated centrosomes. In addition, the p53 positive signal was markedly intense in cells containing aberrant chromosome segregation and micronuclei. Our results suggest that centrosome overduplication may play an important role in the redistribution of the p53 protein in rotenone-treated cells, and this could represent an alternative mechanism of rotenone to induce apoptosis in neuronal cells.

    DOI: 10.1111/j.1440-1819.2006.01526.x

    Web of Science

    researchmap

  • L-DOPA treatment from the viewpoint of neuroprotection - Possible mechanism of specific and progressive dopaminergic neuronal death in Parkinson's disease Reviewed

    Norio Ogawa, Masato Asanuma, Ikuko Miyazaki, FJ Diaz-Corrales, Ko Miyoshi

    JOURNAL OF NEUROLOGY   252   23 - 31   2005.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:DR DIETRICH STEINKOPFF VERLAG  

    With regard to the mechanism of selective dopaminergic neuronal death, experimental results of studies on the neurotoxicity of MPTP and rotenone indicate that degeneration of dopamine neurons is closely related to mitochondrial dysfunction, inflammatory process and oxidative stress, particularly with regard to the generation of quinones as dopamine neuron-specific oxidative stress. Thus, it is now clear that the presence of high levels of discompartmentalized free dopamine in sdopaminergic neurons may explain the specific vulnerability of dopaminergic neurons through the generation of highly toxic quinones.

    DOI: 10.1007/s00415-005-4006-7

    Web of Science

    researchmap

  • Prednisolone Inhibits Proliferation of Cultured Pulmonary Artery Smooth Muscle Cells of Patients With Idiopathic Pulmonary Arterial Hypertension Reviewed

    Aiko Ogawa, Kazufumi Nakamura, Hiromi Matsubara, Hideki Fujio, Tetsuya Ikeda, Kaoru Kobayashi, Ikuko Miyazaki, Masato Asanuma, Katsumasa Miyaji, Daiji Miura, Kengo Fukushima Kusano, Hiroshi Date, Tohru Ohe

    Circulation   112 ( 12 )   1806 - 1812   2005.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Ovid Technologies (Wolters Kluwer Health)  

    <bold>
    <italic>Background—</italic>
    </bold>
    Idiopathic pulmonary arterial hypertension (IPAH) is associated with proliferation of smooth muscle cells (SMCs) in small pulmonary arteries. There is no therapy that specifically inhibits SMC proliferation. Recent studies reported that prednisolone (PSL) inhibits the postangioplasty proliferation of SMCs in atherosclerotic arteries. In this study, we tested the hypothesis that PSL has antiproliferative effects on pulmonary artery SMCs of patients with IPAH.




    <bold>
    <italic>Methods and Results—</italic>
    </bold>
    Pulmonary artery SMCs were harvested from the pulmonary arteries of 6 patients with IPAH who underwent lung transplantation. Control SMCs were obtained from 5 patients with bronchogenic carcinoma who underwent lung lobectomy. After incubation in the presence of platelet-derived growth factor (PDGF), PSL was added at different concentrations and cell proliferation was assessed by
    3
    H-thymidine incorporation. PSL (2×10
    −4
    and 2×10
    −3
    mol/L) significantly inhibited PDGF-stimulated proliferation (
    <italic>P</italic>
    &lt;0.05) of SMCs from patients with IPAH but did not affect cell viability of SMCs, as confirmed by trypan blue staining. In cell cycle analysis using a microscope-based multiparameter laser scanning cytometer, PSL inhibited the progression of SMCs from G
    0
    /G
    1
    to the S phase. This inhibition was associated with increased p27 expression level. PSL (2×10
    −4
    mol/L) also inhibited PDGF-induced SMC migration.




    <bold>
    <italic>Conclusions—</italic>
    </bold>
    Our results indicate that PSL has an antiproliferative effect on cultured SMCs of pulmonary arteries from patients with IPAH and suggest that PSL may be potentially useful therapeutically in patients with IPAH.

    DOI: 10.1161/circulationaha.105.536169

    Web of Science

    researchmap

  • Pramipexole has ameliorating effects on levodopa-induced abnormal dopamine turnover in parkinsonian striatum and quenching effects on dopamine-semiquinone generatedin vitro Reviewed

    Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Masako Shimizu, Ken-ichi Tanaka, Norio Ogawa

    Neurological Research   27 ( 5 )   533 - 539   2005.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Maney Publishing  

    DOI: 10.1179/016164105x22093

    Web of Science

    researchmap

  • Rotenone induces aggregation of γ-tubulin protein and subsequent disorganization of the centrosome: Relevance to formation of inclusion bodies and neurodegeneration Reviewed

    Francisco J. Diaz-Corrales, Masato Asanuma, Ikuko Miyazaki, Ko Miyoshi, Norio Ogawa

    Neuroscience   133 ( 1 )   117 - 135   2005.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.neuroscience.2005.01.044

    Web of Science

    researchmap

  • :-drug dependence and toxicity

    Asanuma Masato, Miyazaki Ikuko

    Folia Pharmacologica Japonica   126, 30-34 ( 1 )   30 - 34   2005

     More details

    Language:Japanese   Publisher:The Japanese Pharmacological Society  

    DOI: 10.1254/fpj.126.30

    CiNii Article

    researchmap

  • Quinone formation as dopaminergic neuron-specific oxidative stress in the pathogenesis of sporadic Parkinson's disease and neurotoxin-induced parkinsonism. Reviewed

    Asanuma Masato, Miyazaki Ikuko, Diaz-Corrales Francisco J, Ogawa Norio

    Acta Medica Okayama   58 ( 5 )   221 - 233   2004.10

     More details

    Language:English   Publisher:Okayama University Medical School  

    DOI: 10.18926/AMO/32105

    Web of Science

    CiNii Article

    CiNii Books

    researchmap

  • Specific Gene Expression and Possible Involvement of Inflammation in Methamphetamine-Induced Neurotoxicity Reviewed

    Masato Asanuma, Ikuko Miyazaki, Youichirou Higashi, Takeshi Tsuji, Norio Ogawa

    Annals of the New York Academy of Sciences   1025 ( 1 )   69 - 75   2004.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1196/annals.1316.009

    Web of Science

    researchmap

  • Expression of metallothionein-III and cell death in differentiated catecholaminergic neuronal cells Reviewed

    Youichirou Higashi, Masato Asanuma, Ikuko Miyazaki, Norio Ogawa

    Neurological Research   26 ( 6 )   671 - 676   2004.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1179/016164104225015895

    Web of Science

    researchmap

  • Parkin attenuates manganese‐induced dopaminergic cell death Reviewed

    Youichirou Higashi, Masato Asanuma, Ikuko Miyazaki, Nobutaka Hattori, Yoshikuni Mizuno, Norio Ogawa

    Journal of Neurochemistry   89 ( 6 )   1490 - 1497   2004.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/j.1471-4159.2004.02445.x

    Web of Science

    researchmap

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1471-4159.2004.02445.x

  • DISC1 localizes to the centrosome by binding to kendrin Reviewed

    Ko Miyoshi, Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Taiichi Katayama, Masaya Tohyama, Norio Ogawa

    Biochemical and Biophysical Research Communications   317 ( 4 )   1195 - 1199   2004.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrc.2004.03.163

    Web of Science

    researchmap

  • Rotenone induces disassembly of the Golgi apparatus in the rat dopaminergic neuroblastoma B65 cell line Reviewed

    Francisco J. Diaz-Corrales, Masato Asanuma, Ikuko Miyazaki, Norio Ogawa

    Neuroscience Letters   354 ( 1 )   59 - 63   2004.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.neulet.2003.09.059

    Web of Science

    researchmap

  • 実験的パーキンソニズムに使われる薬剤〜MPTP,6-ヒドロキシドパミンならびにロテノン.

    浅沼幹人, 宮崎育子, 小川紀雄

    医薬ジャーナル   40,111-116   2004

     More details

  • 遺伝子改変によるパーキンソン病モデル.

    浅沼幹人, 宮崎育子

    脳の科学   292,165-170   2004

     More details

  • ドパミン受容体とトランスポーター.

    小川紀雄, 宮崎育子

    脳の科学   292,53-59   2004

     More details

  • Neuroprotective effects of nonsteroidal anti-inflammatory drugs on neurodegenerative diseases Reviewed

    Masato Asanuma, Ikuko Miyazaki, Norio Ogawa

    CURRENT PHARMACEUTICAL DESIGN   10 ( 6 )   695 - 700   2004

     More details

    Language:English   Publisher:BENTHAM SCIENCE PUBL LTD  

    It is well known that nonsteroidal anti-inflammatory drugs (NSAIDs) possess anti-inflammatory, analgesic and antipyretic properties by inhibiting cyclooxygenase (COX), a prostaglandin-synthesizing enzyme. It has also been revealed that NSAIDs exert inhibitory effects on the generating system of nitric oxide radicals and modulating effects on transcription factors which are related to inflammatory reactions including cytokine expression. Recently, a number of studies have been conducted focusing on the neuroprotective effects of NSAIDs, since it has been reported that inflammatory processes are associated with the pathogenesis of several neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. In the experimental model of Parkinson's disease, NSAIDs have also exerted neuroprotective effects which are based not only on their COX-inhibiting effects but also on other properties: inhibitory effects on nitric oxide synthesis, action as agonists for peroxisome proliferator-activated receptor gamma, and some unknown pharmacological effects. In this article, various pharmacological effects of NSAIDs except their inhibitory action on COX are reviewed, and possible neuroprotective effects of NSAIDs have been discussed on neurodegenerative diseases, especially Parkinson's disease.

    DOI: 10.2174/1381612043453072

    Web of Science

    researchmap

  • PP4-061 抗体アレイを用いた下部尿路閉塞モデルラット膀胱平滑筋層におけるタンパク発現のprofiling(一般演題(ポスター))

    藤田 治, 横山 光彦, 浅沼 幹人, 宮崎 育子, 小川 紀雄, 公文 裕巳

    日本泌尿器科学会雑誌   95 ( 2 )   545 - 545   2004

     More details

    Language:Japanese   Publisher:一般社団法人 日本泌尿器科学会  

    DOI: 10.5980/jpnjurol.95.545_3

    CiNii Article

    researchmap

  • Methamphetamine-induced neurotoxicity in mouse brain is attenuated by ketoprofen, a non-steroidal anti-inflammatory drug Reviewed

    Masato Asanuma, Takeshi Tsuji, Ikuko Miyazaki, Ko Miyoshi, Norio Ogawa

    Neuroscience Letters   352 ( 1 )   13 - 16   2003.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.neulet.2003.08.015

    Web of Science

    researchmap

  • Overexpression of Cu–Zn superoxide dismutase protects neuroblastoma cells against dopamine cytotoxicity accompanied by increase in their glutathione level Reviewed

    M.Emdadul Haque, Masato Asanuma, Youichirou Higashi, Ikuko Miyazaki, Ken-ichi Tanaka, Norio Ogawa

    Neuroscience Research   47 ( 1 )   31 - 37   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0168-0102(03)00166-4

    Web of Science

    researchmap

  • Apoptosis-inducing neurotoxicity of dopamine and its metabolites via reactive quinone generation in neuroblastoma cells Reviewed

    M Emdadul Haque, Masato Asanuma, Youichirou Higashi, Ikuko Miyazaki, Ken-ichi Tanaka, Norio Ogawa

    Biochimica et Biophysica Acta (BBA) - General Subjects   1619 ( 1 )   39 - 52   2003.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0304-4165(02)00440-3

    Web of Science

    researchmap

  • アルツハイマー病脳で減少しているgrowth inhibitory factor (GIF) の加齢にともなう変化.

    浅沼幹人, 宮崎育子, 東 洋一郎, 田中健一, 小川紀雄

    分子精神医学   2003

     More details

  • ドパミンおよびその誘導体のキノン体生成を介したアポトーシス誘導性.

    浅沼幹人, 宮崎育子, Haque ME, 東 洋一郎, 小川紀雄

    Prog Med   2003

     More details

  • 非ステロイド性消炎鎮痛薬の神経保護作用の新展開.

    浅沼幹人, 宮崎育子, 辻 武史, 小川紀雄

    日本神経精神薬理学会雑誌   23,111-119   2003

     More details

  • Dopamine- or L-DOPA-induced neurotoxicity: The role of dopamine quinone formation and tyrosinase in a model of Parkinson's disease Reviewed

    Masato Asanuma, Ikuko Miyazaki, Norio Ogawa

    NEUROTOXICITY RESEARCH   5 ( 3 )   165 - 176   2003

     More details

    Language:English   Publisher:F P GRAHAM PUBLISHING CO  

    Dopamine (DA)- or L-dihydroxyphenylalanine- (L-DOPA-) induced neurotoxicity is thought to be involved not only in adverse reactions induced by long-term L-DOPA therapy but also in the pathogenesis of Parkinson!s disease. Numerous in vitro and in vivo studies concerning DA- or L-DOPA-induced neurotoxicity have been reported in recent decades. The reactive oxygen or nitrogen species generated in the enzymatical oxidation or auto-oxidation of an excess amount of DA induce neuronal damage and/or apoptotic or non-apoptotic cell death; the DA-induced damage is prevented by various intrinsic and extrinsic antioxidants. DA and its metabolites containing two hydroxyl residues exert cytotoxicity in dopaminergic neuronal cells mainly due to the generation of highly reactive DA and DOPA quinones which are dopaminergic neuron-specific cytotoxic molecules. DA and DOPA quinones may irreversibly alter protein function through the formation of 5-cysteinyl-catechols on the proteins. For example, the formation of DA quinone-alpha-synuclein consequently increases cytotoxic protofibrils and the covalent modification of tyrosine hydroxylase by DA quinones. The melanin-synthetic enzyme tyrosinase in the brain may rapidly oxidize excess amounts of cytosolic DA and L-DOPA, thereby preventing slowly progressive cell damage by auto-oxidation of DA, thus maintaning DA levels. Since tyrosinase also possesses catecholamine-synthesizing activity in the absence of tyrosine hydroxylase (TH), the double-edged synthesizing and oxidizing functions of tyrosinase in the dopaminergic system suggest its potential for application in the synthesis of DA, instead of TH in the degeneration of dopaminergic neurons, and in the normalization of abnormal DA turnover in long-term L-DOPA-treated Parkinson's disease patients.

    DOI: 10.1007/BF03033137

    Web of Science

    researchmap

  • The p53-activated Gene, PAG608, Requires a Zinc Finger Domain for Nuclear Localization and Oxidative Stress-induced Apoptosis Reviewed

    Youichirou Higashi, Masato Asanuma, Ikuko Miyazaki, M. Emdadul Haque, Naoko Fujita, Ken-ichi Tanaka, Norio Ogawa

    Journal of Biological Chemistry   277 ( 44 )   42224 - 42232   2002.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1074/jbc.m203594200

    Web of Science

    researchmap

  • Aggravation of 6-hydroxydopamine-induced dopaminergic lesions in metallothionein-I and -II knock-out mouse brain Reviewed

    Masato Asanuma, Ikuko Miyazaki, Youichirou Higashi, Ken-ichi Tanaka, Md Emdadul Haque, Naoko Fujita, Norio Ogawa

    Neuroscience Letters   327 ( 1 )   61 - 65   2002.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0304-3940(02)00346-4

    Web of Science

    researchmap

  • The dopamine agonist cabergoline provides neuroprotection by activation of the glutathione system and scavenging free radicals Reviewed

    Michiyo Yoshioka, Ken-ichi Tanaka, Ikuko Miyazaki, Naoko Fujita, Youichirou Higashi, Masato Asanuma, Norio Ogawa

    Neuroscience Research   43 ( 3 )   259 - 267   2002.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0168-0102(02)00040-8

    Web of Science

    researchmap

  • Methamphetamine-induced increase in striatal p53 DNA-binding activity is attenuated in Cu,Zn-superoxide dismutase transgenic mice Reviewed

    Masato Asanuma, Ikuko Miyazaki, Youichirou Higashi, Jean Lud Cadet, Norio Ogawa

    Neuroscience Letters   325 ( 3 )   191 - 194   2002.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0304-3940(02)00291-4

    Web of Science

    researchmap

  • GPI1046 prevents dopaminergic dysfunction by activating glutathione system in the mouse striatum Reviewed

    Ken-ichi Tanaka, Michiyo Yoshioka, Ikuko Miyazaki, Naoko Fujita, Norio Ogawa

    Neuroscience Letters   321 ( 1-2 )   45 - 48   2002.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0304-3940(01)02547-2

    Web of Science

    researchmap

  • Inhibition of Tyrosinase Reduces Cell Viability in Catecholaminergic Neuronal Cells Reviewed

    Youichirou Higashi, Masato Asanuma, Ikuko Miyazaki, Norio Ogawa

    Journal of Neurochemistry   75 ( 4 )   1771 - 1774   2002.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1046/j.1471-4159.2000.0751771.x

    Web of Science

    researchmap

  • ニューロメラニン合成酵素チロシナーゼのドパミン神経障害への関与.

    浅沼幹人, 東 洋一郎, 宮崎育子, 小川紀雄

    Prog Med   2002

     More details

  • Chronic cerebral hypoperfusion induces striatal alterations due to the transient increase of NO production and the depression of glutathione content Reviewed

    Ken-ichi Tanaka, Naoko Wada-Tanaka, Ikuko Miyazaki, Masahiko Nomura, Norio Ogawa

    Neurochemical Research   27 ( 4 )   331 - 336   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1023/a:1014967414468

    Web of Science

    researchmap

  • Neuroprotective effects of non-steroidal anti-inflammatory drugs by direct scavenging of nitric oxide radicals Reviewed

    Masato Asanuma, Sakiko Nishibayashi-Asanuma, Ikuko Miyazaki, Masahiro Kohno, Norio Ogawa

    Journal of Neurochemistry   76 ( 6 )   1895 - 1904   2001.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1046/j.1471-4159.2001.00205.x

    Web of Science

    researchmap

  • Progressive cortical atrophy after forebrain ischemia in diabetic rats Reviewed

    Fumio Kondo, Masato Asanuma, Ikuko Miyazaki, Yoichi Kondo, Ken-ichi Tanaka, Hirohumi Makino, Norio Ogawa

    Neuroscience Research   39 ( 3 )   339 - 346   2001.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0168-0102(00)00233-9

    Web of Science

    researchmap

  • Localization, regulation, and function of metallothionein-III/growth inhibitory factor in the brain. Reviewed

    Aoki Sogawa Chiharu, Asanuma Masato, Sogawa Norio, Miyazaki Ikuko, Nakanishi Tohru, Furuta Hiroaki, Ogawa Noriko

    Acta Medica Okayama   55 ( 1 )   1 - 9   2001.2

     More details

    Language:English   Publisher:Okayama University Medical School  

    DOI: 10.18926/AMO/32031

    Web of Science

    CiNii Article

    CiNii Books

    researchmap

  • Molecular mechanism in activation of glutathione system by ropinirole, a selective dopamine D2 agonist Reviewed

    Ken-ichi Tanaka, Ikuko Miyazaki, Naoko Fujita, Md Emdadul Haque, Masato Asanuma, Norio Ogawa

    Neurochemical Research   26 ( 1 )   31 - 36   2001

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1023/a:1007672414239

    Web of Science

    researchmap

  • Antioxidants protect against dopamine-induced metallothionein-III (GIF) mRNA expression in mouse glial cell line (VR-2g) Reviewed

    Chiharu Aoki Sogawa, Ikuko Miyazaki, Norio Sogawa, Masato Asanuma, Norio Ogawa, Hiroaki Furuta

    Brain Research   853 ( 2 )   310 - 316   2000.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0006-8993(99)02284-2

    Web of Science

    researchmap

  • Dopamine D2 receptor-mediated antioxidant and neuroprotective effects of ropinirole, a dopamine agonist Reviewed

    Motoyuki Iida, Ikuko Miyazaki, Ken-Ichi Tanaka, Hideaki Kabuto, Emi Iwata-Ichikawa, Norio Ogawa

    Brain Research   838 ( 1-2 )   51 - 59   1999.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Recent information suggests that free radicals are closely involved in the pathogenesis and/or progression of Parkinson's disease (PD). High-dose levodopa therapy has been suggested to increase oxidative stress, thereby accelerating the progression of PD. Based on this viewpoint, free radical scavenging, antioxidant and neuroprotective agents which may prevent the progression of PD have recently attracted considerable attention. For example, ergot derivative dopamine (DA) agonists have been reported to scavenge free radicals in vitro and show a neuroprotective effect in vivo. Non-ergot DA agonists have also recently been used in the treatment of PD despite the lack of substantial evidence for any free radical scavenging activity or antioxidant activity. The present study was conducted to assess the in vitro free radical scavenging and antioxidant activities of ropinirole, a non-ergot DA agonist, as well as its glutathione (GSH), catalase and superoxide dismutase (SOD) activating effects and neuroprotective effect in vivo. Ropinirole scavenges free radicals and suppresses lipid peroxidation in vitro, but these activities are very weak, suggesting that the antioxidant effect of ropinirole observed in vitro may be a minor component of its neuroprotective effect in vivo. Administration of ropinirole for 7 days increased GSH, catalase and SOD activities in the striatum and protected striatal dopaminergic neurons against 6- hydroxydopamine (6-OHDA) in mice. Pre-treatment with sulpiride prevented ropinirole from enhancing striatal GSH, catalase and SOD activities and abolished the protection of dopaminergic neurons against 6-OHDA. Our findings indicate that activation of GSH, catalase and SOD mediated via DA D2 receptors may be the principal mechanism of neuroprotection by ropinirole.

    DOI: 10.1016/S0006-8993(99)01688-1

    Web of Science

    Scopus

    PubMed

    researchmap

  • Early treatment with cyclosporin A ameliorates the reduction of muscarinic acetylcholine receptors in gerbil hippocampus after transient forebrain ischemia Reviewed

    Yoichi Kondo, Masato Asanuma, Emi Iwata, Fumio Kondo, Ikuko Miyazaki, Norio Ogawa

    Neurochemical Research   24 ( 1 )   9 - 13   1999

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1023/a:1020915727119

    Web of Science

    researchmap

  • Glial cells protect neurons against oxidative stress via transcriptional up-regulation of the glutathione synthesis Reviewed

    Emi Iwata-Ichikawa, Yoichi Kondo, Ikuko Miyazaki, Masato Asanuma, Norio Ogawa

    Journal of Neurochemistry   72 ( 6 )   2334 - 2344   1999

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We examined the effects of oxidative stress on rat cultured mesencephalic neurons and glial cells. Glial cells were more resistant to 6- hydroxydopamine (6-OHDA) and H2O2 toxicity than neurons. In glial cells, incubation with 6-OHDA and H2O2 induced a significant increase in the expression of γ-glutamylcysteine synthetase (the rate-limiting enzyme in glutathione synthesis) mRNA, which correlated well with increased TPA- response element (TRE)-binding activity. Furthermore, a subsequent elevation in cellular total glutathione content was also observed. In neurons, both agents decreased TRE-binding activity, and these cells failed to up-regulate the glutathione synthesis. We also examined the mechanisms of the neuroprotective effects of glial cells using a gila conditioned medium. Neurons maintained in gila conditioned medium up-regulated the level of TRE- binding activity, γ-glutamylcysteine synthetase mRNA expression, and total glutathione content in response to 6-OHDA or H2O2, and became more resistant to both agents than cells maintained in a normal medium. Neurons maintained in normal medium failed to up-regulate the glutathione synthesis. Our results suggest that transcriptional up-regulation of glutathione synthesis in glial cell appears to mediate brain glial cell resistance against oxidative stress, and that glial cells protect neurons via transcriptional up-regulation of the antioxidant system.

    DOI: 10.1046/j.1471-4159.1999.0722334.x

    Scopus

    PubMed

    researchmap

  • Protective effects of nicergoline against hydrogen peroxide toxicity in rat neuronal cell line Reviewed

    Emi Iwata, Ikuko Miyazaki, Masato Asanuma, Azusa Iida, Norio Ogawa

    Neuroscience Letters   251 ( 1 )   49 - 52   1998.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0304-3940(98)00489-3

    Web of Science

    researchmap

  • Effects of repeated cyclosporin A administration on iminodipropionitrile-induced dyskinesia and TRE-/CRE-binding activities in rat brain Reviewed

    Kyoko Iida, Emi Iwata, Masato Asanuma, Sakiko N Asanuma, Marvin Gómez-Vargas, Ikuko Miyazaki, Tohru Nakanishi, Norio Ogawa

    Neuroscience Research   30 ( 2 )   185 - 193   1998.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0168-0102(97)00128-4

    Web of Science

    researchmap

  • Aniracetam ameriorates impaired pre-and post-synaptic cholinergic indices in gerbil hippocampus induced by transient forebrain ischemia. Reviewed

    Yoichi Kondo, Masato Asanuma, Hideaki Kabuto, Emi Iwata, Fumio Kondo, Ikuko Miyazaki, Norio Ogawa

    J. Brain Sci.   23   250 - 260   1997

     More details

    Language:English  

    researchmap

▼display all

Books

  • アストロサイトの亜鉛関連分子を標的としたパーキンソン病治療戦略

    宮崎育子, 浅沼幹人

    日本薬理学雑誌  2021 

     More details

  • パーキンソン病での神経保護標的としてのアストロサイトの抗酸化分子

    浅沼幹人, 宮崎育子

    日本薬理学雑誌  2021 

     More details

  • パーキンソン病と亜鉛結合蛋白

    浅沼幹人, 宮崎育子(生命金属ダイナミクス)

    エヌ・ティー・エス  2021 

     More details

  • 金属結合蛋白とパーキンソン病

    宮崎育子

    NEURODIEM JAPAN  2020 

     More details

  • 脳内環境辞典 Nrf2.

    メディカルドウ  2017 

     More details

  • アストロサイトの部位特異的プロファイルがもたらす脳内環境と神経保護

    浅沼幹人, 宮崎育子( Role: Joint author)

    メディカルドウ  2014 

     More details

  • 実践行動薬理学

    金芳堂  2010 

     More details

  • Handbook of Free Radicals: Formation, Types and Effects

    Nova Science Publishers  2010 

     More details

  • 酸化ストレス─フリーラジカル医学生物学の最前線 Ver.2

    医歯薬出版  2006 

     More details

▼display all

MISC

  • メタロチオネイン欠損による脳梁形成不全の増悪

    正井加織, 菊岡 亮, 名越 武, 十川千春, 十川紀夫, 宮崎育子, 浅沼幹人

    日本解剖学会第75回中国・四国支部学術集会抄録集   2021.10

     More details

    Language:Japanese  

    researchmap

  • アストロサイト−ミクログリア連関を介したロテノン誘発ドパミン神経障害へのメタロチオネインの関与

    宮崎育子, 菊岡 亮, 磯岡奈未, 村上真樹, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2021抄録集   2021.10

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • メタロチオネイン欠損マウスにおける脳梁形成不全

    正井加織, 菊岡 亮, 名越 武, 十川千春, 十川紀夫, 宮崎育子, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2021抄録集   2021.10

     More details

    Language:Japanese  

    researchmap

  • LPS投与による機械的刺激反応閾値低下における金属結合タンパク質メタロチオネインの関与

    十川紀夫, 奥村雅代, 宮崎育子, 富田美穂子, 金銅英二, 十川千春, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2021抄録集   2021.10

     More details

    Language:Japanese  

    researchmap

  • ロチゴチンによるアストロサイトを標的としたドパミン神経保護

    宮崎育子

    ニュープロパッチ 8周年記念講演会資料   2021.10

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese  

    researchmap

  • 妊娠・授乳期エポキシ樹脂BADGE曝露による新生仔マウス脳発達異常におけるエストロゲンβレセプターの関与

    浅沼幹人, 宮崎育子, 西山千春, 菊岡亮, 名越 武, Kyle Quin, 禅正和真

    第43回日本生物学的精神医学会・第51回日本神経精神薬理学会合同年会プログラム・抄録集   2021.7

     More details

    Language:Japanese  

    researchmap

  • アストロサイト-ミクログリア連関がもたらす農薬ロテノン誘発ドパミン神経障害

    宮崎育子, 菊岡 亮, 磯岡奈未, 村上真樹, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第43回日本生物学的精神医学会・第51回日本神経精神薬理学会合同年会プログラム・抄録集   2021.7

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • アストロサイトにおけるメタロチオネインを標的としたドパミン神経保護. シンポジウム: 生体金属部会シンポジウム 〜メタロチオネイン機能の新たな展開〜

    宮崎育子, 浅沼幹人

    第48回日本毒性学会学術年会抄録集   2021.7

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese  

    researchmap

  • 中脳グリア細胞を介したロテノン誘発ドパミン神経特異的神経障害とそのメカニズムに関する検討

    宮崎育子, 菊岡 亮, 磯岡奈未, 村上真樹, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第15回パーキンソン病・運動障害疾患コングレス (MDSJ)プログラム・抄録集   2021.7

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • Rotenone-induced dopaminergic neurotoxicity mediated by astrocyte-microglia interaction

    Miyazaki, I, Kikuoka, R, Isooka, N, Murakami, S, Sogawa, C, Sogawa, N, Kitamura, Y, Asanuma, M

    第62回日本神経学会学術大会プログラム・抄録集   2021.5

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • nvolvement of estrogen receptor  in the abnormal brain development in fetuses by maternal bisphenol A diglycidyl ether exposure during gestation and lactation

    Ikuko Miyazaki, Chiharu Nishiyama, Ryo Kikuoka, Takeru Nagoshi, Kyle E. Quin, Nami Isooka, Kazumasa Zensho, Masato Asanuma

    2021.3

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • Astrocyte-microglia interaction promotes rotenone-induced dopaminergic neurotoxicity

    Ikuko Miyazaki, Ryo Kikuoka, Nami Isooka, Shinki Murakami, Chiharu Sogawa, Norio Sogawa, Yoshihisa Kitamura, Masato Asanuma

    2021.3

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • 5-HT1Aアゴニストによるアストロサイトのメタロチオネイン発現誘導とドパミン神経保護

    宮崎育子, 磯岡奈未, 菊岡 亮, 北村佳久, 浅沼幹人

    第14回パーキンソン病・運動障害疾患コングレス (MDSJ)プログラム・抄録集   2021.2

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • ロテノン誘発ドパミン神経障害におけるアストロサイト−ミクログリア連関

    宮崎育子, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2020抄録集   2020.11

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • パーキンソン病モデルマウスにおける杜仲抽出物のメタロチオネイン発現誘導および神経保護効果

    今福史智, Jin Sun, 磯岡奈未, 上舞 直, 清水崇司, 豊田俊明, 岡本裕成, 菊岡 亮, 宮崎育子, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2020抄録集   2020.11

     More details

    Language:Japanese  

    researchmap

  • アストロサイトのセロトニン1A受容体を標的としたドパミン神経保護20

    宮崎育子

    第61回日本神経学会学術大会プログラム・抄録集   2020.9

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese  

    researchmap

  • Region-specific glial dysfunction promotes rotenone neurotoxicity

    Miyazaki, I, Kikuoka, R, Isooka, N, Sogawa, C, Sogawa, N, Kitamura, Y, Asanuma, M

    2020.8

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • 農薬ロテノン曝露によるアストロサイトの部位特異的反応性の差異と神経細胞に及ぼす影響

    宮崎育子, 浅沼幹人, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久

    第125回日本解剖学会総会抄録集   2020.3

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • 神経保護標的としてのアストロサイトのグルタチオン

    浅沼幹人, 宮崎育子

    第93回日本薬理学会年会抄録集   2020.3

     More details

    Authorship:Last author   Language:English  

    researchmap

  • アストロサイトの亜鉛関連分子を標的としたパーキンソン病治療戦略

    宮崎育子, 浅沼幹人

    第93回日本薬理学会年会抄録集   2020.3

     More details

    Authorship:Lead author, Corresponding author   Language:English  

    researchmap

  • ロテノン誘発部位特異的アストロサイト機能不全によるドパミン神経障害へのメタロチオネインの関与

    宮崎育子, 浅沼幹人, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2019抄録集   2019.10

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • ロテノン投与パーキンソン病モデルにおけるコーヒー成分のメタロチオネイン発現誘導と神経保護効果

    磯岡奈未, 宮崎育子, 和田晃一, 菊岡 亮, 古川智英子, 北村佳久, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2019抄録集   2019.10

     More details

    Language:Japanese  

    researchmap

  • 老齢メタロチオネインノックアウトマウスにおける脳組織学的変化

    菊岡 亮, 野村昌紀, 磯岡奈未, 宮崎育子, 十川紀夫, 十川千春, 北村佳久, 浅沼幹人

    日本毒性学会生体金属部会メタルバイオサイエンス研究会2019抄録集   2019.10

     More details

    Language:Japanese  

    researchmap

  • Involvement of region-specific glial dysfunction in rotenone neurotoxicity

    Miyazaki, I, Asanuma, M, Murakami, S, Kikuoka, R, Isooka, N, Sogawa, C, Sogawa, N, Kitamura, Y

    VI AsCNP2019 Congress Abstract   2019.10

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • 部位特異的アストロサイト機能不全がもたらすロテノン誘発ドパミン神経障害

    宮崎 育子, 浅沼 幹人, 村上 真樹, 菊岡 亮, 磯岡 奈未, 十川 千春, 十川 紀夫, 北村 佳久

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   13回   118 - 118   2019.7

     More details

    Authorship:Lead author   Language:Japanese   Publisher:Movement Disorder Society of Japan (MDSJ)  

    researchmap

  • ドパミン神経毒による酸化ストレスに対するアストロサイトの分子発現および神経保護作用の部位特異性

    浅沼 幹人, 奥村 菜央, 鳥越, 育子, 村上 真樹, 北村 佳久, 千堂 年昭

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   13回   118 - 118   2019.7

     More details

    Language:Japanese  

    researchmap

  • Region-specific features of astrocytes against dopaminergic neurotoxin-induced oxidative stress

    Asanuma, M, Okumura-Torigoe, N, Miyazaki, I, Murakami, S, Kitamura, Y, Sendo, T

    2019.7

     More details

    Language:English  

    researchmap

  • Regional differences in reaction of astrocytes against rotenone contribute to dopaminergic neurodegeneration

    Miyazaki, I, Asanuma, M, Murakami, S, Kikuoka, R, Isooka, N, Sogawa, C, Sogawa, N, Kitamura, Y

    2019.7

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • 妊娠・授乳期のエポキシ樹脂曝露が新生仔マウスに及ぼす行動毒性

    宮崎育子, 菊岡 亮, 磯岡奈未, 禅正和真, 新居 麗, 園部奏生, 船越英丸, 中山恵利香, 進 浩太郎, 山本大地, Kyle Quin, 浅沼幹人

    第46回日本毒性学会学術年会抄録集   2019.6

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • Rotigotine protects dopaminergic neurons via astrocytic serotonin 1A receptors

    Miyazaki, I, Isooka, N, Kikuoka, R, Wada, K, Nakayama, E, Shin, K, Yamamoto, D, Kitamura, Y, Asanuma, M

    2019.5

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • 妊娠・授乳期にエポキシ樹脂曝露した新生仔マウスの脳発達に関する組織学的・行動学的解析

    宮崎育子, 菊岡 亮, 磯岡奈未, 禅正和真, 新居 麗, 園部奏生, 船越英丸, 中山恵利香, 進 浩太郎, 山本大地, Kyle Quin, 浅沼幹人

    第124回日本解剖学会総会抄録集   2019.3

     More details

    Authorship:Lead author   Language:Japanese  

    researchmap

  • Treatment with coffee ingredients protects central and myenteric neurons in parkinsonian model

    Miyazaki, I, Isooka, N, Wada, K, Kikuoka, R, Kitamura, Y, Asanuma, M

    2019.3

     More details

    Authorship:Lead author   Language:English  

    researchmap

  • 大学院生が期待するウェットからドライな薬学研究の未来 抗がん剤投与による不安症状の発症機序解明とその治療・予防に向けたアプローチ

    住吉 佑介, 内藤 七海, 中村 優花, 菅 詩歩, 牛尾 聡一郎, 宮崎 育子, 浅沼 幹人, 北村 佳久, 千堂 年昭

    日本薬学会年会要旨集   139年会 ( 1 )   329 - 329   2019.3

     More details

    Language:Japanese   Publisher:(公社)日本薬学会  

    researchmap

  • ロチゴチンによるアストロサイトのセロトニン1Aレセプターを標的としたドパミン神経保護

    宮崎 育子, 磯岡 奈未, 菊岡 亮, 和田 晃一, 中山 恵利香, 進 浩太郎, 山本 大地, 北村 佳久, 浅沼 幹人

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   12回   117 - 117   2018.7

     More details

    Language:Japanese   Publisher:Movement Disorder Society of Japan (MDSJ)  

    researchmap

  • 医療のさらなる安心・安全を志向する若手研究者のイノベーティブチャレンジ 抗がん剤投与による不安症状発症の病態機序解明 セロトニン神経系の関与

    中村 優花, 住吉 佑介, 内藤 七海, 牛尾 聡一郎, 宮崎 育子, 浅沼 幹人, 北村 佳久, 千堂 年昭

    日本薬学会年会要旨集   138年会 ( 1 )   333 - 333   2018.3

     More details

    Language:Japanese   Publisher:(公社)日本薬学会  

    researchmap

  • 医療のさらなる安心・安全を志向する若手研究者のイノベーティブチャレンジ 抗がん剤投与による不安症状発症の病態機序解明 セロトニン神経系の関与

    中村 優花, 住吉 佑介, 内藤 七海, 牛尾 聡一郎, 宮崎 育子, 浅沼 幹人, 北村 佳久, 千堂 年昭

    日本薬学会年会要旨集   138年会 ( 1 )   333 - 333   2018.3

     More details

    Language:Japanese   Publisher:(公社)日本薬学会  

    researchmap

  • 農薬ロテノン暴露による腸管神経障害とコーヒー成分による神経保護効果

    宮崎 育子, 磯岡 奈未, 菊岡 亮, 和田 晃一, 北村 佳久, 浅沼 幹人

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   11回   99 - 99   2017.10

     More details

    Language:Japanese   Publisher:Movement Disorder Society of Japan (MDSJ)  

    researchmap

  • 農薬ロテノンによるアストロサイト機能異常がもたらすドパミン神経障害

    宮崎 育子, 菊岡 亮, 磯岡 奈未, 北村 佳久, 浅沼 幹人

    日本生物学的精神医学会・日本神経精神薬理学会合同年会プログラム・抄録集   39回・47回   158 - 158   2017.9

     More details

    Language:Japanese   Publisher:日本生物学的精神医学会・日本神経精神薬理学会  

    researchmap

  • アストロサイトを介したミルタザピンのドパミン神経保護効果に関する検討

    菊岡 亮, 宮崎 育子, 久保田 菜月, 前田 恵実, 香川 大樹, 守山 雅晃, 粂 明日香, 北村 佳久, 浅沼 幹人

    日本生物学的精神医学会・日本神経精神薬理学会合同年会プログラム・抄録集   39回・47回   193 - 193   2017.9

     More details

    Language:Japanese   Publisher:日本生物学的精神医学会・日本神経精神薬理学会  

    researchmap

  • 農薬ロテノンによる非細胞自律性ドパミン神経障害

    宮崎 育子, 村上 真樹, 菊岡 亮, 磯岡 奈未, 北村 佳久, 浅沼 幹人

    The Journal of Toxicological Sciences   42 ( Suppl. )   S321 - S321   2017.6

     More details

    Language:Japanese   Publisher:(一社)日本毒性学会  

    researchmap

  • 革新的創薬・育薬を目指す若手研究者によるトランスレーショナルリサーチの最前線 パーキンソン病モデルマウスにおけるミルタザピンの神経保護効果に関する検討

    菊岡 亮, 宮崎 育子, 浅沼 幹人, 北村 佳久, 千堂 年昭

    日本薬学会年会要旨集   137年会 ( 1 )   284 - 284   2017.3

     More details

    Language:Japanese   Publisher:(公社)日本薬学会  

    researchmap

  • ALSモデルの運動障害および運動神経変性に対するセロトニン1Aアゴニストの抑制効果

    浅沼幹人, 宮崎育子, 村上真樹, 村上真樹, 鳥越菜央, 中野剛志, 菊岡亮, 北村佳久, 千堂年昭

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   10th   68 - 68   2016.10

     More details

    Language:Japanese   Publisher:Movement Disorder Society of Japan (MDSJ)  

    J-GLOBAL

    researchmap

  • 農薬ロテノン暴露によるドパミン神経障害におけるアストロサイトの関与

    宮崎育子, 村上真樹, 村上真樹, 菊岡亮, 磯岡奈未, 北村佳久, 浅沼幹人

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   10th   69 - 69   2016.10

     More details

    Language:Japanese   Publisher:Movement Disorder Society of Japan (MDSJ)  

    J-GLOBAL

    researchmap

  • 抗うつ薬ミルタザピンのアストロサイトを標的としたドパミン神経保護(Astrocyte-targeted neuroprotection of antidepressant mirtazapine)

    宮崎 育子, 菊岡 亮, 久保田 菜月, 前田 恵実, 香川 大樹, 守山 雅晃, 粂 明日香, 村上 真樹, 北村 佳久, 浅沼 幹人

    日本神経精神薬理学会年会プログラム・抄録集   46回   232 - 232   2016.7

     More details

    Language:English   Publisher:(一社)日本神経精神薬理学会  

    researchmap

  • 筋萎縮性側索硬化症モデルにおける運動障害および運動神経変性に対する8-OH-DPATの抑制効果(8-OH-DPAT ameliorates motor dysfunction and motor neuron degeneration in mouse model of amyotrophic lateral sclerosis)

    浅沼 幹人, 宮崎 育子, 村上 真樹, 鳥越 奈央, 中野 剛志, 菊岡 亮, 北村 佳久, 千堂 年昭

    日本神経精神薬理学会年会プログラム・抄録集   46回   232 - 232   2016.7

     More details

    Language:English   Publisher:(一社)日本神経精神薬理学会  

    researchmap

  • パーキンソン病モデルマウスにおけるミルタザピンの神経保護効果に関する検討(Neuroprotective effects of mirtazapine in parkinsonian mice)

    菊岡 亮, 宮崎 育子, 久保田 菜月, 前田 恵実, 香川 大樹, 守山 雅晃, 粂 明日香, 村上 真樹, 北村 佳久, 浅沼 幹人

    日本神経精神薬理学会年会プログラム・抄録集   46回   232 - 232   2016.7

     More details

    Language:English   Publisher:(一社)日本神経精神薬理学会  

    researchmap

  • パーキンソン病モデルマウスにおけるアストロサイトの5‐HT1Aレセプターを標的とした神経保護

    宮崎育子, 宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 村上真樹, 村上真樹, 北村佳久, 浅沼幹人, 浅沼幹人

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集   9th   67 - 67   2015.10

     More details

    Language:Japanese   Publisher:Movement Disorder Society of Japan (MDSJ)  

    J-GLOBAL

    researchmap

  • ミルタザピンのアストロサイトを標的としたドパミン神経保護効果

    宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 村上真樹, 村上真樹, 北村佳久, 浅沼幹人, 浅沼幹人

    日本神経精神薬理学会プログラム・抄録集   45th   185 - 185   2015.9

     More details

    Language:Japanese   Publisher:日本生物学的精神医学会・日本神経精神薬理学会  

    J-GLOBAL

    researchmap

  • ALSモデルマウスにおけるセロトニン1Aアゴニストによる神経保護効果の検討

    中野剛志, 鳥越菜央, 村上真樹, 宮崎育子, 浅沼幹人, 北村佳久, 千堂年昭

    日本薬学会年会要旨集(CD-ROM)   135th ( 3 )   ROMBUNNO.28PB-PM242 - 191   2015.3

     More details

    Language:Japanese   Publisher:(公社)日本薬学会  

    J-GLOBAL

    researchmap

  • アストロサイトを介したレベチラセタムのドパミン神経保護効果に関する検討

    宮崎育子, 村上真樹, 鳥越菜央, 北村佳久, 浅沼幹人

    日本薬理学会近畿部会プログラム・要旨集   125th   37   2014.5

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • ACTH反復投与ラットにおけるketamineの抗うつ効果に関する検討

    中村紘子, 米田紗緒里, 野白有里子, 宮崎育子, 浅沼幹人, 北村佳久, 千堂年昭

    日本薬学会年会要旨集(CD-ROM)   134th ( 3 )   ROMBUNNO.28PMM-105 - 151   2014.3

     More details

    Language:Japanese   Publisher:(公社)日本薬学会  

    J-GLOBAL

    researchmap

  • ALSモデルマウスにおけるセロトニン1Aアゴニストによる神経保護効果の検討

    中野剛志, 鳥越菜央, 村上真樹, 宮崎育子, 浅沼幹人, 北村佳久, 千堂年昭

    日本生物学的精神医学会誌   154   2014

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Neuroprotective effects of levetiracetam in parkinsonian model mice

    Ikuko Miyazaki, Shinki Murakami, Nao Torigoe, Yoshihisa Kitamura, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   124   150P - 150P   2014

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • 抗がん剤投与ラットにおける精神機能および海馬神経新生に関する検討

    米田紗緒里, 服部紗代, 中村紘子, 宮崎育子, 浅沼幹人, 北村佳久, 千堂年昭

    日本薬学会年会要旨集(CD-ROM)   134th   ROMBUNNO.GS02-7   2014

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • ドパミン欠乏による線条体ニューロンの1次繊毛の伸長

    三好耕, 三好耕, 松崎伸介, 松崎伸介, 松崎伸介, 宮崎育子, 浅沼幹人, 片山泰一

    日本脳科学会プログラム・講演抄録集   41st   2014

  • 線条体アストロサイトが酸化ストレスに対して発現誘導する因子の網羅的解析

    鳥越 菜央, 宮崎 育子, 村上 真樹, 北村 佳久, 千堂 年昭, 浅沼 幹人

    日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集   23回・43回   250 - 250   2013.10

     More details

    Language:Japanese   Publisher:日本臨床精神神経薬理学会・日本神経精神薬理学会  

    researchmap

  • セロトニン1Aアゴニストによるアストロサイトにおけるメタロチオネイン発現誘導とドパミン神経保護

    宮崎 育子, 村上 真樹, 竹島 美香, 鳥越 菜央, 三好 耕, 北村 佳久, 浅沼 幹人

    日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集   23回・43回   251 - 251   2013.10

     More details

    Language:Japanese   Publisher:日本臨床精神神経薬理学会・日本神経精神薬理学会  

    researchmap

  • ラットの行動変化から推察する抗がん剤投与による精神機能変化―ドキソルビシンおよびシクロホスファミド投与による影響―

    北村佳久, 服部紗代, 米田沙緒里, 三宅彩香, 小山敏広, 宮崎育子, 浅沼幹人, 千堂年昭

    日本サイコオンコロジー学会総会プログラム・抄録集   26th   132   2013

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Neuroprotective effects of serotonin-1a agonist 8-OH-DPAT target astrocytes

    Ikuko Miyazaki, Shinki Murakami, Mika Takeshima, Nao Torigoe, Ko Miyoshi, Yoshihisa Kitamura, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   121   138P - 138P   2013

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Effect of the 5-HT1A receptor function on antidepressive effect and neurogenesis in ACTH treated rats Reviewed

    Miyake Ayaka, Kitamura Yoshihisa, Hattori Sayo, Koyama Toshihiro, Miyazaki Ikuko, Asanuma Masato, Sendo Toshiaki

    JOURNAL OF PHARMACOLOGICAL SCIENCES   121   144P   2013

  • Influence of doxorubicin and cyclophosphamide on psychological behavior in rats Reviewed

    Kitamura Yoshihisa, Hattori Sayo, Miyake Ayaka, Koyama Toshihiro, Miyazaki Ikuko, Asanuma Masato, Sendo Toshiaki

    JOURNAL OF PHARMACOLOGICAL SCIENCES   121   143P   2013

  • ニューロンの1次繊毛の解析

    三好耕, 松崎伸介, 松崎伸介, 宮崎育子, 浅沼幹人, 片山泰一

    日本脳科学会プログラム・講演抄録集   40th   2013

  • Investigation on the Mechanisms for the Suppression of Cell Proliferation in the Dentate Gyrus of the Hippocampus in ACTH Treated Rats

    Hiromi Hayashi, Maho Doi, Yuka Onoue, Keiko Kuwatsuka, Ayaka Miyake, Toshihiro Koyama, Kazuaki Shinomiya, Ikuko Miyazaki, Masato Aasanuma, Yoshihisa Kitamura

    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN   132 ( 2 )   173 - 178   2012.2

     More details

    Language:Japanese   Publishing type:Book review, literature introduction, etc.   Publisher:PHARMACEUTICAL SOC JAPAN  

    We previously reported that adrenocorticotropic hormone (ACTH) -treated rats serve as a valuable animal model for tricyclic antidepressant-resistant depressive conditions. The present study was undertaken to investigate the changes in neurogenesis in the hippocampus of ACTH-treated rats. Chronic treatment of ACTH decreased the number of bromodeoxyuridine-labeled cells in the dentate gyrus, and the coadministration of imipramine and lithium, and electroconvulsive stimuli recovered these reductions. Furthermore, chronic ACTH treatment also decreased the expression of brain-derived neurotrophic factor, and the coadministration of imipramine and lithium, and electroconvulsive stimuli recovered these reductions. These results suggest that antidepressant-resistant depression is caused by the suppression of neurogenesis, and the coadministration of imipramine and lithium, and electroconvulsive stimuli exert an antidepressant-like effect by recovering proliferative signals and neurogenesis.

    Web of Science

    researchmap

  • Chronic rotenone exposure affects enteric glial cells

    Shinki Murakami, Ikuko Miyazaki, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   118   188P - 188P   2012

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Neuroprotective effects of serotonin-1a agonist 8-OH-DPAT in parkinsonian mice

    Ikuko Miyazaki, Shinki Murakami, Mika Takeshima, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   118   111P - 111P   2012

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • ACTH反復投与ラットにおける海馬細胞新生の減少およびそのメカニズムに関する検討

    林宏美, 土居真穂, 尾上由華, 鍬塚圭子, 三宅彩香, 小山敏広, 四宮一昭, 宮崎育子, 浅沼幹人, 北村佳久

    日本薬学会年会要旨集   131st ( 1 )   257   2011.3

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Effects of rotenone exposure on primary cultured enteric neuronal or glial cells

    Ikuko Miyazaki, Shinki Murakami, Mika Takeshima, Ko Miyoshi, Masato Asanuma

    NEUROSCIENCE RESEARCH   71   E190 - E190   2011

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2011.07.820

    Web of Science

    researchmap

  • Dopamine receptors localize to the neuronal primary cilium, a non-synaptic neurotransmission device

    Ko Miyoshi, Kyosuke Kasahara, Shinki Murakami, Ikuko Miyazaki, Masato Asanuma

    NEUROSCIENCE RESEARCH   71   E87 - E87   2011

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2011.07.372

    Web of Science

    researchmap

  • Effects of imipramine and lithium on the suppression of cell proliferation in the dentate gyrus of the hippocampus in ACTH treated rats

    Hiromi Hayashi, Maho Doi, Yuka Onoue, Keiko Kuwatsuka, Ayaka Miyake, Toshihiro Koyama, Kazuaki Shinomiya, Ikuko Miyazaki, Masato Asanuma, Yoshihisa Kitamura

    JOURNAL OF PHARMACOLOGICAL SCIENCES   115   251P - 251P   2011

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Effects of chronic rotenone exposure on enteric neuronal or glial cells in vivo

    Shinki Murakami, Ikuko Miyazaki, Mika Takeshima, Ko Miyoshi, Masato Asanuma

    NEUROSCIENCE RESEARCH   71   E190 - E190   2011

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2011.07.821

    Web of Science

    researchmap

  • Dopamine-specific metallothionein induction in reactive astrocytes through dopamine transporter

    Ikuko Miyazaki, Yuri Kikkawa, Mika Takeshima, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   91P - 91P   2010

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • L-theanine up-regulates glutathione levels in astrocytes and protects excess dopamine-induced neurotoxicity

    Mika Takeshima, Ikuko Miyazaki, Yuri Kikkawa, Taizo Kita, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   54P - 54P   2010

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Effects of docosahexaenoic acid on methamphetamine-induced neurotoxicity in cultured dopaminergic neuronal cells

    Maiko Murata, Natsuho Urasoe, Ikuko Miyazaki, Masato Asanuma, Taizo Kita

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   139P - 139P   2010

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Lithium elongates neuronal primary cilia

    Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Masato Asanuma

    NEUROSCIENCE RESEARCH   68   E84 - E84   2010

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2010.07.138

    Web of Science

    researchmap

  • Astrocytes protect dopaminergic neurons from dopamine quinone toxicity

    Ikuko Miyazaki, Yuri Kikkawa, Mika Takeshima, Ko Miyoshi, Taizo Kita, Masato Asanuma

    NEUROSCIENCE RESEARCH   68   E347 - E347   2010

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2010.07.1537

    Web of Science

    researchmap

  • Involvement of nicotinic acetylcholine receptor on the conditioned place aversion behavior and c-Fos expression induced by naloxone in single-dose morphine-treated rats Reviewed

    Ikuta Yuichi, Ishida Shigeru, Miyazaki Ikuko, Asanuma Masato, Araki Hiroaki, Matsunaga Hisashi, Kitamura Yoshihisa, Sendo Toshiaki

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   187P   2010

  • Effects of cell proliferation and astroglial activity by the chronic treatment of ACTH in the hippocampal dentate gyrus of adult rats Reviewed

    Doi Maho, Nagamachi Tomoko, Egawa Maki, Miyazaki Ikuko, Kawasaki Hiromu, Sendo Toshiaki, Asanuma Masato, Kitamura Yoshihisa

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   178P   2010

  • REDUCTION OF PPAR-gamma IN METHAMPHETAMINE-INDUCED NEUROTOXICITY AND PROTECTIVE EFFECTS OF INTERFERON-gamma

    Masato Asanuma, Ikuko Miyazaki, Yuri Kikkawa, Mika Takeshima, Ko Miyoshi, Taizo Kita

    JOURNAL OF NEUROCHEMISTRY   110   49 - 49   2009.9

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:WILEY-BLACKWELL PUBLISHING, INC  

    Web of Science

    researchmap

  • メタンフェタミン神経毒性に対するインターフェロンγおよびPPARγアゴニストの保護効果(Protective effects of interferon-γ or peroxisome proliferator-activated receptor-γ agonist against methamphetamine-induced neurotoxicity)

    浅沼 幹人, 宮崎 育子, 福岡 早紀, 穂積 宏彰, 辻 武史, 北村 佳久, 千堂 年昭, 喜多 大三

    神経化学   48 ( 2-3 )   214 - 214   2009.6

     More details

    Language:English   Publisher:日本神経化学会  

    researchmap

  • Effects of L-theanine on dopaminergic neuronal cells

    Megumi Someya, Mika Takeshima, Maiko Murata, Ikuko Miyazaki, Masato Asanuma, Taizo Kita

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   277P - 277P   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Cyclooxygenase-independent neuroprotective effects of aspirin against dopamine quinone-induced neurotoxicity

    Yuri Kikkawa, Naotaka Kimoto, Saki Fukuoka, Ikuko Miyazaki, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   279P - 279P   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Reactive astrocyte-specific induction of metallothionein in methamphetamine-induced neurotoxicity

    Ikuko Miyazaki, Yuri Kikkawa, Mika Takeshima, Ko Miyoshi, Taizo Kita, Masato Asanuma

    NEUROSCIENCE RESEARCH   65   S256 - S256   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2009.09.1458

    Web of Science

    researchmap

  • Effects of docosahexaenoic acid on dopaminergic neuronal cells

    Maiko Murata, Mika Takeshima, Megumi Someya, Ikuko Miyazaki, Masato Asanuma, Taizo Kita

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   278P - 278P   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Analysis of mice carrying a 129 substrain-derived deletion variant in Disc1

    Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Masato Asanuma

    NEUROSCIENCE RESEARCH   65   S122 - S122   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2009.09.577

    Web of Science

    researchmap

  • Early changes in astrocytes after rotenone exposure

    Naotaka Kimoto, Saki Fukuoka, Yuri Kikkawa, Francisco. J. Diaz-Corrales, Ikuko Miyazaki, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   129P - 129P   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Involvement of PPAR-gamma in methamphetamine-induced neurotoxicity and protective effect of interferon-gamma Reviewed

    Fukuoka Saki, Hozumi Hiroaki, Kimoto Naotaka, Kikkawa Yuri, Tsuji Takeshi, Miyazaki Ikuko, Kitamura Yoshihisa, Sendo Toshiaki, Kita Taizo, Asanuma Masato

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   220P   2009

  • Effects of dopamine transporter expression on treatment of ACTH in rats Reviewed

    Emoto Sayaka, Kitamura Yoshihisa, Miyazaki Ikuko, Kitagawa Kouhei, Nagamachi Tomoko, Doi Maho, Ishimaru Yui, Asanuma Masato, Sendo Toshiaki

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   162P   2009

  • メタンフェタミン急性神経毒性に対するインターフェロン‐γの保護効果発現機序に関する検討

    福岡早紀, 浅沼幹人, 宮崎育子, 穂積宏彰, 吉川友理, 木本直孝, 北村佳久, 千堂年昭, 喜多大三

    日本薬理学雑誌   132 ( 3 )   16P   2008.9

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • メタンフェタミン神経毒性に対するインターフェロンγの保護効果におけるペルオキシソーム増殖剤活性化受容体γ(PPARγ)の関与(Possible involvement of peroxisome proliferator-activated receptor-γ in protective effects of interferon-γ against methamphetamine-induced neurotoxicity)

    浅沼 幹人, 福岡 早紀, 穂積 宏彰, 宮崎 育子, 北村 佳久, 千堂 年昭, 喜多 大三

    神経化学   47 ( 2-3 )   237 - 237   2008.8

     More details

    Language:English   Publisher:日本神経化学会  

    researchmap

  • [New perspectives on the mechanism of methamphetamine-induced neurotoxicity]. Reviewed

    Kita T, Takeshima M, Wagner GC, Hozumi H, Miyazaki I, Asanuma M

    Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology   28 ( 2 )   49 - 61   2008.4

  • メタンフェタミン急性神経毒性に対するインターフェロン‐γの保護効果発現機序に関する検討

    福岡早紀, 浅沼幹人, 宮崎育子, 穂積宏彰, 吉川友理, 木本直孝, 北村佳久, 千堂年昭, 喜多大三

    日本薬理学会近畿部会プログラム・要旨集   113rd   38   2008

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Effect of baicalin, flavone glucuronide on dopaminergic neuronal cells

    Mika Takeshirna, Yumiko Tanaka, Megurni Someya, Maiko Murata, Ikuko Miyazaki, Masato Asanuma

    JOURNAL OF PHARMACOLOGICAL SCIENCES   106   93P - 93P   2008

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Functional analysis of neuronal cilia using pericentrin mutant mice

    Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Masato Asanuma

    NEUROSCIENCE RESEARCH   61   S207 - S207   2008

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    Web of Science

    researchmap

  • Protective effects of interferon-gamma against methamphetamine-induced neurotoxicity

    Hiroaki Hozumi, Masato Asanuma, Ikuko Miyazaki, Saki Fukuoka, Yuri Kikkawa, Naotaka Kimoto, Yoshihisa Kitamura, Toshiaki Sendou, Taizou Kita, Yutaka Gomita

    JOURNAL OF PHARMACOLOGICAL SCIENCES   106   175P - 175P   2008

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

    researchmap

  • Effects of chronic ACTH treatment on astrocytes and neurogenesis in adult rat hippocampus Reviewed

    Nagamachi Tomoko, Miyazaki Ikuko, Emoto Sayaka, Do Maho, Kawasaki' Hiromu, Asanuma Masato, Kitamura Yoshihisa, Sendo Toshiaki, Gomita Yutaka

    JOURNAL OF PHARMACOLOGICAL SCIENCES   106   188P   2008

  • Expression profiling of inflammatory molecules related to methamphetamine-induced neurotoxicity

    27 ( 5 )   294 - 294   2007.11

     More details

  • Glutathione synthesis-related molecule in astrocytes in dopamine quinone neurotoxicity

    27 ( 5 )   289 - 289   2007.11

     More details

  • Effects of bisphenol A on cultured monoaminergic neuronal cells

    27 ( 5 )   299 - 299   2007.11

     More details

  • Psychic dependence liability and neurotoxicity of uncontrolled newly-abused drugs (law-evading drugs) : evaluation of phenethylamines

    FUNADA Masahiko, AOO Naoya, ASANUMA Masato, MIYAZAKI Ikuko, HANAJIRI KIKURA Ruri, GODA Yukihiro, WADA Kiyoshi

    42 ( 4 )   226 - 227   2007.8

     More details

  • メチルフェニデートのドパミン神経毒性に対する作用

    日名俊行, 浅沼幹人, 喜多大三, 宮崎育子, 小川紀雄, 北村佳久, 千堂年昭, 五味田裕

    日本薬学会年会要旨集   127th ( 2 )   129   2007.3

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Functional analysis of neuronal primary cilia using pericentrin mutant mice

    Ko Miyoshi, Ikuko Miyazaki, Masato Asanuma

    NEUROSCIENCE RESEARCH   58   S123 - S123   2007

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    Web of Science

    researchmap

  • Pericentrin is localized to the base of neuronal primary cilia in the developing cerebral cortex

    Ko Miyoshi, Ikuko Miyazaki, Masato Asanuma

    NEUROSCIENCE RESEARCH   55   S233 - S233   2006

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    Web of Science

    researchmap

  • Specific expression of proapoptotic factor PAG608 on motor neurons in spinal cords of L-DOPA-treated parkinsonian models

    Ikuko Miyazaki, Masako Shimizu, Francisco J. Diaz-Corrales, Maria F. Esraba-Alba, Masato Asanuma

    NEUROSCIENCE RESEARCH   55   S201 - S201   2006

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    Web of Science

    researchmap

  • Aggregation of parkin protein in the centrosome and accumulation of cyclin E/cdk 2 complex in CATH.a cells treated with dopamine

    F. J. Diaz-Corrales, M. Asanuma, I. Miyazaki, K. Miyoshi, N. Ogawa

    MOVEMENT DISORDERS   21   S580 - S581   2006

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:WILEY-LISS  

    Web of Science

    researchmap

  • Quinone formation in acute methamphetamine-induced neurotoxicity

    MIYAZAKI Ikuko, ASANUMA Masato, DIAZ-CORRALES Francisco J, MIYOSHI Ko, SHIMIZU Masako, KITAICHI Kiyoyuki, OGAWA Norio

    25 ( 6 )   342 - 342   2005.12

     More details

  • マンガン誘発神経毒性におけるパーキン蛋白のドパミン神経特異的な関与

    宮崎 育子, 浅沼 幹人, 東 洋一郎, 服部 信孝, 水野 美邦, 小川 紀雄

    日本神経精神薬理学雑誌 = Japanese journal of psychopharmacology   23 ( 6 )   2003.12

  • Rotenone induces fragmentation of Golgi apparatus in dopaminergic neuroblastoma cell line

    DIAZ Francisco, ASANUMA Masato, MIYAZAKI Ikuko, OGAWA Norio

    23 ( 6 )   311 - 311   2003.12

     More details

  • Complemental effects of tyrosinase, melanin synthetic enzyme, on dopamine containing neurons

    ASANUMA Masato, MIYAZAKI Ikuko, HIGASHI Youichirou, TANAKA Ken-ichi, OGAWA Norio

    22 ( 6 )   360 - 360   2002.12

     More details

  • Apoptosis-inducing toxicity of L-DOPA and dopamine via quinone generation

    OGAWA Norio, HAQUE M. Emdadul, MIYAZAKI Ikuko, HIGASHI Youichirou, ASANUMA Masato

    22 ( 6 )   363 - 363   2002.12

     More details

  • Studies on expression of dopamine receptors and signal transduction in astrocytes

    MIYAZAKI Ikuko, ASANUMA Masato, HIGASHI Youichirou, TSUJI Takeshi, OGAWA Norio

    22 ( 6 )   364 - 364   2002.12

     More details

  • Effects of non-steroidal anti-inflammatory drugs on methamphetamine-induced neurotoxicity

    TSUJI Takeshi, ASANUMA Masato, MIYAZAKI Ikuko, TANAKA Ken-ichi, OGAWA Norio

    22 ( 6 )   292 - 292   2002.12

     More details

  • Dopamine agonist ropinirole exerts antioxidant and neuroprotective effects via D2 receptors

    OGAWA Norio, TANAKA Kin-ichi, MIYAZAKI Ikuko, ASANUMA Masato, IIDA Motoyuki

    19 ( 6 )   313 - 313   1999.12

     More details

  • Time course of 6-hydroxydopamine-induced changes of anti-oxidant in the mouse brain

    TANAKA Ken-ichi, MIYAZAKI Ikuko, FUJITA Naoko, ASANUMA Masato, OGAWA Norio

    19 ( 6 )   312 - 312   1999.12

     More details

▼display all

Presentations

  • Region-specific glial dysfunction promotes rotenone neurotoxicity.

    Miyazaki, I, Kikuoka, R, Isooka, N, Sogawa, C, Sogawa, N, Kitamura, Y, Asanuma, M

    第61回日本神経学会学術大会  2020.8.31 

     More details

    Event date: 2020.8.31 - 2020.9.2

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 農薬ロテノン曝露によるアストロサイトの部位特異的反応性の差異と神経細胞に及ぼす影響

    宮崎育子, 浅沼幹人, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久

    第125回日本解剖学会総会  2020.3.25 

     More details

    Event date: 2020.3.25 - 2020.3.27

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトの亜鉛関連分子を標的としたパーキンソン病治療戦略 Invited

    宮崎育子, 浅沼幹人

    第93回日本薬理学会年会  2020.3.16 

     More details

    Event date: 2020.3.16 - 2020.3.18

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • Involvement of region-specific glial dysfunction in rotenone neurotoxicity.

    Miyazaki, I, Asanuma, M, Murakami, S, Kikuoka, R, Isooka, N, Sogawa, C, Sogawa, N, Kitamura, Y

    VI AsCNP2019  2019.10.11 

     More details

    Event date: 2019.10.11 - 2019.10.13

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Regional differences in reaction of astrocytes against rotenone contribute to dopaminergic neurodegeneration.

    Miyazaki, I, Asanuma, M, Murakami, S, Kikuoka, R, Isooka, N, Sogawa, C, Sogawa, N, Kitamura, Y

    14th European Meeting on Glial Cells in Health and Disease  2019.7.10 

     More details

    Event date: 2019.7.9 - 2019.7.13

    Language:English   Presentation type:Poster presentation  

    researchmap

  • アストロサイト-ミクログリア連関がもたらす農薬ロテノン誘発ドパミン神経障害

    宮崎育子, 菊岡 亮, 磯岡奈未, 村上真樹, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第43回日本生物学的精神医学会・第51回日本神経精神薬理学会合同年会  2021.7.14 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトにおけるメタロチオネインを標的としたドパミン神経保護 Invited

    宮崎育子, 浅沼幹人

    第48回日本毒性学会学術年会  2021.7.9 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • 中脳グリア細胞を介したロテノン誘発ドパミン神経特異的神経障害とそのメカニズムに関する検討

    宮崎育子, 菊岡 亮, 磯岡奈未, 村上真樹, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第15回パーキンソン病・運動障害疾患コングレス (MDSJ)  2021.7.2 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Rotenone-induced dopaminergic neurotoxicity mediated by astrocyte-microglia interaction.

    Miyazaki, I, Kikuoka, R, Isooka, N, Murakami, S, Sogawa, C, Sogawa, N, Kitamura, Y, Asanuma, M

    2021.5.19 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 妊娠・授乳期エポキシ樹脂曝露による新生仔マウスの脳発達異常へのエストロゲン受容体βの関与

    宮崎育子, 西山千春, 菊岡 亮, 名越 武, Kyle Quin, 磯岡奈未, 禅正和真, 浅沼幹人

    第126回日本解剖学会総会  2021.3.28 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • アストロサイト−ミクログリア連関がもたらすロテノン誘発ドパミン神経障害

    宮崎育子, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第94回日本薬理学会年会  2021.3.8 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 低用量ロテノン慢性皮下投与による新規パーキンソン病モデルの確立

    宮崎育子, 磯岡奈未, 今福 史智, Jin Sun, 菊岡 亮, 古川智英子, 浅沼幹人

    第46回岡山脳研究セミナー  2021.1.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロテノン誘発ドパミン神経障害におけるアストロサイト−ミクログリア連関

    宮崎育子, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    メタルバイオサイエンス研究会2020  2020.11.6 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • アストロサイトのセロトニン1A受容体を標的としたドパミン神経保護 Invited

    宮崎育子

    第61回日本神経学会学術大会  2020.9.1 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    researchmap

  • 部位特異的アストロサイト機能不全がもたらすロテノン誘発ドパミン神経障害

    宮崎育子, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第45回岡山脳研究セミナー  2020.1.28 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロチゴチンによるアストロサイトのセロトニン1Aレセプターを標的としたドパミン神経保護 Invited

    宮崎育子

    パーキンソン病講演会  2019.8.23 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    researchmap

  • アストロサイトにおけるメタロチオネイン発現を標的とした神経保護 Invited

    宮崎育子, 浅沼幹人

    メタルバイオサイエンス研究会2018  2018.11.17 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • メタロチオネイン欠損マウスにおける脳梁形成不全

    正井加織, 菊岡 亮, 名越 武, 十川 千春, 十川 紀夫, 宮崎 育子, 浅沼 幹人

    日本毒性学会生体金属部会 メタルバイオサイエンス研究会2021  2021.10.27 

     More details

    Event date: 2021.10.27 - 2021.10.28

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイト−ミクログリア連関を介したロテノン誘発ドパミン神経障害へのメタロチオネインの関与

    宮崎育子, 菊岡 亮, 磯岡奈未, 村上真樹, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    日本毒性学会生体金属部会 メタルバイオサイエンス研究会2021  2021.10.27 

     More details

    Event date: 2021.10.27 - 2021.10.28

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • LPS投与による機械的刺激反応閾値低下における金属結合タンパク質メタロチオネインの関与

    十川紀夫, 奥村雅代, 宮崎育子, 富田美穂子, 銅 英二, 十川千春, 浅沼 幹人

    日本毒性学会生体金属部会 メタルバイオサイエンス研究会2021  2021.10.27 

     More details

    Event date: 2021.10.27 - 2021.10.28

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン誘発ドパミン神経障害におけるアストロサイト−ミクログリア連関

    宮崎育子, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久, 浅沼幹人

    第14回パーキンソン病・運動障害疾患コングレス (MDSJ)  2021.2.22 

     More details

    Event date: 2021.2.22 - 2021.2.24

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 神経保護標的としてのアストロサイトのグルタチオン Invited

    浅沼幹人, 宮崎育子

    第93回日本薬理学会年会  2020.3.16 

     More details

    Event date: 2020.3.16 - 2020.3.18

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • 老齢メタロチオネインノックアウトマウスにおける脳組織学的変化

    菊岡 亮, 野村昌紀, 磯岡奈未, 宮崎育子, 十川紀夫, 十川千春, 北村佳久, 浅沼幹人

    メタルバイオサイエンス研究会2019  2019.10.29 

     More details

    Event date: 2019.10.29 - 2019.10.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン誘発部位特異的アストロサイト機能不全によるドパミン神経障害へのメタロチオネインの関与

    宮崎育子, 浅沼幹人, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久

    メタルバイオサイエンス研究会2019  2019.10.29 

     More details

    Event date: 2019.10.29 - 2019.10.30

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロテノン投与パーキンソン病モデルにおけるコーヒー成分のメタロチオネイン発現誘導と神経保護効果

    磯岡奈未, 宮崎育子, 和田晃一, 菊岡 亮, 古川智英子, 北村佳久, 浅沼幹人

    メタルバイオサイエンス研究会2019  2019.10.29 

     More details

    Event date: 2019.10.29 - 2019.10.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 部位特異的アストロサイト機能不全がもたらすロテノン誘発ドパミン神経障害

    宮崎育子, 浅沼幹人, 村上真樹, 菊岡 亮, 磯岡奈未, 十川千春, 十川紀夫, 北村佳久

    第13回パーキンソン病・運動障害疾患コングレス (MDSJ)  2019.7.27 

     More details

    Event date: 2019.7.25 - 2019.7.27

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミン神経毒による酸化ストレスに対するアストロサイトの分子発現および神経保護作用の部位特異性

    浅沼幹人, 奥村, 鳥越, 菜央, 宮崎育子, 村上真樹, 北村佳久, 千堂年昭

    第13回パーキンソン病・運動障害疾患コングレス (MDSJ)  2019.7.27 

     More details

    Event date: 2019.7.25 - 2019.7.27

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Region-specific features of astrocytes against dopaminergic neurotoxin-induced oxidative stress.

    Asanuma, M, Okumura-Torigoe, N, Miyazaki, I, Murakami, S, Kitamura, Y, Sendo, T

    14th European Meeting on Glial Cells in Health and Disease  2019.7.10 

     More details

    Event date: 2019.7.9 - 2019.7.13

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 妊娠・授乳期のエポキシ樹脂曝露が新生仔マウスに及ぼす行動毒性

    宮崎育子, 菊岡 亮, 磯岡奈未, 禅正和真, 新居 麗, 園部奏生, 船越英丸, 中山恵利香, 進 浩太郎, 山本大地, Kyle Quin, 浅沼幹人

    第46回日本毒性学会学術年会  2019.6.27 

     More details

    Event date: 2019.6.27 - 2019.6.28

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Rotigotine protects dopaminergic neurons via astrocytic serotonin 1A receptors.

    Miyazaki, I, Isooka, N, Kikuoka, R, Wada, K, Nakayama, E, Shin, K, Yamamoto, D, Kitamura, Y, Asanuma, M

    第60回日本神経学会学術大会  2019.5.24 

     More details

    Event date: 2019.5.23 - 2019.5.25

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 妊娠・授乳期にエポキシ樹脂曝露した新生仔マウスの脳発達に関する組織学的•行動学的解析

    宮崎育子, 菊岡 亮, 磯岡奈未, 禅正和真, 新居 麗, 園部奏生, 船越英丸, 中山恵利香, 進 浩太郎, 山本大地, Kyle Quin, 浅沼幹人

    第124回日本解剖学会総会  2019.3.27 

     More details

    Event date: 2019.3.27 - 2019.3.29

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Treatment with coffee ingredients protects central and myenteric neurons in parkinsonian model.

    Miyazaki, I, Isooka, N, Wada, K, Kikuoka, R, Kitamura, Y, Asanuma, M

    第92回日本薬理学会年会  2019.3.15 

     More details

    Event date: 2019.3.14 - 2019.3.15

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 妊娠・授乳期エポキシ樹脂暴露産仔マウス脳への影響に関する組織学的・行動学的検討

    浅沼幹人, 宮崎育子, 禅正和真, 菊岡 亮, 磯岡奈未, 新居 麗, 園部奏生, 船越英丸, 中山恵利香, 進 浩太郎, 山本大地, Kyle Quin

    日本解剖学会第73回中国・四国支部学術集会  2018.10.20 

     More details

    Event date: 2018.10.20 - 2018.10.21

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Neuroprotective effects of coffee ingredients against rotenone induced neurodegeneration in parkinsonian model.

    Miyazaki, I, Isooka, N, Kikuoka, R, Wada, K, Kitamura, Y, Asanuma, M

    22nd International Congress of Parkinson’s Disease and Movement Disorders  2018.10.6 

     More details

    Event date: 2018.10.5 - 2018.10.8

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Rotigotine protects dopaminergic neurons by targeting serotonin 1A receptors on astrocytes.

    Asanuma, M, Miyazaki, I, Isooka, N, Kikuoka, R, Wada, K, Nakayama, E, Shin, K, Yamamoto, D, Kitamura, Y

    22nd International Congress of Parkinson’s Disease and Movement Disorders  2018.10.6 

     More details

    Event date: 2018.10.5 - 2018.10.8

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 母体へのエポキシ樹脂BADGE暴露が新生仔マウス脳にもたらす影響

    宮崎育子, 菊岡 亮, 磯岡奈未, 中山恵利香, 進 浩太郎, 山本大地, Quin E. Kyle, 船越英丸, 禅正和真, 浅沼幹人

    第45回日本毒性学会学術年会  2018.7.19 

     More details

    Event date: 2018.7.19 - 2018.7.20

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロチゴチンによるアストロサイトのセロトニン1Aレセプターを標的としたドパミン神経保護

    宮崎育子, 磯岡奈未, 菊岡 亮, 和田晃一, 中山恵利香, 進 浩太郎, 山本大地, 北村佳久, 浅沼幹人

    第12回パーキンソン病・運動障害疾患コングレス (MDSJ)  2018.7.7 

     More details

    Event date: 2018.7.5 - 2018.7.7

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Involvement of enhanced 5-HT2A receptor function on doxorubicin and cyclophosphamide-induced anxiety-like behavior in rats.

    Naito, N, Kitamura, Y, Nakamura, Y, Sumiyoshi, Y, Kan, S, Miyazaki, I, Asanuma, M, Sendo, T

    31st The International College of Neuropsychopharmacology (CINP World Congress)  2018.6.16 

     More details

    Event date: 2018.6.16 - 2018.6.19

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Influence of benzodiazepine medications and GABAA receptor function on the pentobarbital-induced sleep behavior of lipopolysaccharide-treated mice.

    Kitamura, Y, Okada, A, Hongo, S, Otsuki, K, Miki, A, Miyazaki, I, Asanuma, M, Sendo, T

    31st The International College of Neuropsychopharmacology (CINP World Congress)  2018.6.16 

     More details

    Event date: 2018.6.16 - 2018.6.19

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Possible involvement of metallothionein in rotenone-induced dopaminergic neurotoxicity.

    Miyazaki, I, Kikuoka, R, Isooka, N, Murakami, S, Kitamura, Y, Asanuma, M

    第59回日本神経学会学術大会  2018.5.24 

     More details

    Event date: 2018.5.23 - 2018.5.26

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 妊娠・授乳期におけるエポキシ樹脂曝露の新生仔マウス脳への影響.

    宮崎育子, 菊岡 亮, 磯岡奈未, 船越英丸, Kyle Quin, 禅正和真, 村上真樹, 竹島美香, 三好 耕, 浅沼幹人

    第123回日本解剖学会総会  2018.3.28 

     More details

    Event date: 2018.3.28 - 2018.3.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトの亜鉛関連分子を標的とした神経病態修飾薬 Invited

    浅沼幹人, 宮崎育子

    日本薬学会第138年会  2018.3.27 

     More details

    Event date: 2018.3.27 - 2018.3.28

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • Mirtazapine exerts astrocyte-mediated dopaminergic neuroprotection.

    Kikuoka, R, Miyazaki, I, Kubota, N, Maeda, M, Kagawa, D, Moriyama, M, Kume, A, Murakami, S, Kitamura, Y, Asanuma, M

    47th Annual Meeting of Society for Neuroscience (Neuroscience 2017)  2017.11.11 

     More details

    Event date: 2017.11.11 - 2017.11.15

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Effects of mirtazapine on doxorubicin and cyclophosphamide-induced spatial cognitive impairment and anxiety-like behavior in rats.

    Nakamura, Y, Kitamura, Y, Naito, N, Sumiyoshi, Y, Miyazaki, I, Asanuma, M, Sendo, T

    47th Annual Meeting of Society for Neuroscience (Neuroscience 2017)  2017.11.11 

     More details

    Event date: 2017.11.11 - 2017.11.15

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 農薬ロテノン暴露による腸管神経障害とコーヒー成分による神経保護.

    宮崎育子, 磯岡奈未, 菊岡 亮, 和田晃一, 北村佳久, 浅沼幹人

    第11回パーキンソン病・運動障害疾患コングレス (MDSJ)  2017.10.28 

     More details

    Event date: 2017.10.26 - 2017.10.28

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 農薬ロテノンによる非細胞自律性ドパミン神経障害へのメタロチオネインの関与.

    宮崎育子, 村上真樹, 菊岡 亮, 磯岡奈未, 北村佳久, 十川千春, 十川紀夫, 浅沼幹人

    メタルバイオサイエンス研究会2017  2017.10.13 

     More details

    Event date: 2017.10.13 - 2017.10.14

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 抗うつ薬ミルタザピンによるアストロサイトのメタロチオネイン発現誘導がもたらすドパミン神経保護

    菊岡 亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 北村佳久, 浅沼幹人

    メタルバイオサイエンス研究会2017  2017.10.13 

     More details

    Event date: 2017.10.13 - 2017.10.14

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 磯岡奈未, 和田晃一, 宮崎育子, 古川智英子, 浅沼幹人

    パーキンソン病モデルにおけるコーヒー成分の神経保護効果とメタロチオネイン発現誘導

    メタルバイオサイエンス研究会2017  2017.10.13 

     More details

    Event date: 2017.10.13 - 2017.10.14

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 農薬ロテノンによるアストロサイト機能異常がもたらすドパミン神経障害.

    宮崎育子, 菊岡 亮, 磯岡奈未, 北村佳久, 浅沼幹人

    第39回日本生物学的精神医学会・第47回日本神経精神薬理学会合同年会  2017.9.29 

     More details

    Event date: 2017.9.28 - 2017.9.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • クロロゲン酸のPDモデルマウスにおける神経保護効果.

    磯岡奈未, 和田晃一, 宮崎育子, 浅沼幹人

    第39回日本生物学的精神医学会・第47回日本神経精神薬理学会合同年会  2017.9.28 

     More details

    Event date: 2017.9.28 - 2017.9.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトを介したミルタザピンのドパミン神経保護に関する検討

    菊岡 亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 村上真樹, 北村佳久, 浅沼幹人

    第39回日本生物学的精神医学会・第47回日本神経精神薬理学会合同年会  2017.9.28 

     More details

    Event date: 2017.9.28 - 2017.9.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Rotenone-induced dopaminergic neurotoxicity promoted by mesencephalic astrocyte dysfunction.

    Miyazaki, I, Murakami, S, Kikuoka, R, Isooka, N, Kitamura, Y, Asanuma, M

    23rd World Congress of Neurology  2017.9.18 

     More details

    Event date: 2017.9.17 - 2017.9.20

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective effects of rotigotine against dopaminergic neurodegeneration by targeting astrocytes.

    Asanuma, M, Miyazaki, I, Isooka, N, Kikuoka, R, Wada, K, Nakayama, E, Shin, K, Yamamoto, D, Kitamura, Y

    23rd World Congress of Neurology  2017.9.17 

     More details

    Event date: 2017.9.17 - 2017.9.20

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 農薬ロテノンによる非細胞自律性ドパミン神経障害.

    宮崎育子, 村上真樹, 菊岡 亮, 磯岡奈未, 北村佳久, 浅沼幹人

    第44回日本毒性学会学術年会  2017.7.10 

     More details

    Event date: 2017.7.10 - 2017.7.12

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • エポキシ樹脂BADGEの培養神経細胞への影響.

    浅沼幹人, 宮崎育子, 竹島美香, 中山恵利香, 進 浩太郎, Quin E. Kyle

    第44回日本毒性学会学術年会  2017.7.10 

     More details

    Event date: 2017.7.10 - 2017.7.12

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective effects of antidepressant mirtazapine against dopaminergic neurodegeneration in cultured cells and in parkinsonian mice possibly by targeting astrocytes.

    Asanuma, M, Miyazaki, I, Kikuoka, R, Murakami, S, Isooka, N, Kitamura, Y

    21st International Congress of Parkinson’s Disease and Movement Disorders  2017.6.5 

     More details

    Event date: 2017.6.4 - 2017.6.8

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Rotenone induces astrocyte-mediated non-cell autonomous dopaminergic neurotoxicity.

    Miyazaki, I, Murakami, S, Kikuoka, R, Isooka, N, Kitamura, Y, Asanuma, M

    21st International Congress of Parkinson’s Disease and Movement Disorders  2017.6.5 

     More details

    Event date: 2017.6.4 - 2017.6.8

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 妊娠・授乳期におけるエポキシ樹脂暴露の産仔脳1次繊毛への影響.

    浅沼幹人, 宮崎育子, 竹島美香, 村上 真樹, 三好 耕

    第122回日本解剖学会総会・全国学術集会  2017.3.30 

     More details

    Event date: 2017.3.28 - 2017.3.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • セロトニン1Aアゴニストによる1次繊毛への影響.

    宮崎育子, 磯岡奈未, 粂 明日香, 三好 耕, 浅沼幹人

    第122回日本解剖学会総会・全国学術集会  2017.3.28 

     More details

    Event date: 2017.3.28 - 2017.3.30

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective effects of rotigotine in parkinsonian mice.

    Isooka, N, Miyazaki, I, Kikuoka, R, Wada, K, Nakayama, E, Yamamoto, D, Shin, K, Kitamura, Y, Asanuma, M

    第90回日本薬理学会年会  2017.3.16 

     More details

    Event date: 2017.3.15 - 2017.3.17

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Rotenone-induced dopaminergic neurotoxicity mediated by astrocytes.

    Miyazaki, I, Murakami, S, Kikuoka, R, Isooka, N, Kitamura, Y, Asanuma, M

    第90回日本薬理学会年会  2017.3.16 

     More details

    Event date: 2017.3.15 - 2017.3.17

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 農薬ロテノン暴露によるドパミン神経障害におけるアストロサイトの関与.

    宮崎育子, 村上真樹, 菊岡 亮, 磯岡奈未, 北村佳久, 浅沼幹人

    第10回パーキンソン病・運動障害疾患コングレス  2016.10.6 

     More details

    Event date: 2016.10.6 - 2016.10.8

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ALSモデルの運動障害および運動神経変性に対するセロトニン1Aアゴニストの抑制効果.

    浅沼幹人, 宮崎育子, 村上真樹, 鳥越奈央, 中野剛志, 菊岡 亮, 北村佳久, 千堂年昭

    第10回パーキンソン病・運動障害疾患コングレス  2016.10.6 

     More details

    Event date: 2016.10.6 - 2016.10.8

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおけるミルタザピンの神経保護効果に関する検討.

    菊岡亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 村上真樹, 北村佳久, 浅沼幹人

    第46回日本神経精神薬理学会年会  2016.7.3 

     More details

    Event date: 2016.7.1 - 2016.7.3

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 抗うつ薬ミルタザピンのアストロサイトを標的としたドパミン神経保護.

    宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 村上真樹, 北村佳久, 浅沼幹人

    第46回日本神経精神薬理学会年会  2016.7.3 

     More details

    Event date: 2016.7.1 - 2016.7.3

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 筋萎縮性側索硬化症モデルにおける運動障害および運動神経変性に対する8-OH-DPATの抑制効果.

    浅沼幹人, 宮崎育子, 村上真樹, 鳥越奈央, 中野剛志, 菊岡 亮, 北村佳久, 千堂年昭

    第46回日本神経精神薬理学会年会  2016.7.3 

     More details

    Event date: 2016.7.1 - 2016.7.3

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Mirtazapine protects dopaminergic neurons via astrocytes.

    Miyazaki, I., Kikuoka, R., Kubota, N., Maeda, M., Kagawa, D., Moriyama, M., Kume, A., Murakami, S., Kitamura, Y., Asanuma, M.

    2016.5.21 

     More details

    Event date: 2016.5.18 - 2016.5.21

    Language:English   Presentation type:Poster presentation  

    researchmap

  • セロトニン1Aアゴニストによるアストロサイト1次繊毛の伸長.

    宮崎育子, 粂明日香, 三好耕, 浅沼幹人

    第121回日本解剖学会総会・全国学術集会  2016.3.29 

     More details

    Event date: 2016.3.28 - 2016.3.29

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Serotonin 1A receptors on astrocytes as a target for dopaminergic neuroprotection.

    Miyazaki, I, Kikuoka, R, Kubota, N, Maeda, M, Kagawa, D, Moriyama, M, Kume, A, Murakami, S, Kitamura, Y, Asanuma, M

    2016.3.11 

     More details

    Event date: 2016.3.9 - 2016.3.11

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective effect of fermented papaya preparation (SAIDO-PS501) via activation of astroglial anti-oxidative system.

    Murakami, S, Miyazaki, I, Asanuma, M

    2016.3.10 

     More details

    Event date: 2016.3.9 - 2016.3.11

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Effects of nicotine on doxorubicin and cyclophosphamide-induced spatial cognition and anxiety in rats

    Sugimoto, M, Kanemoto, E, Watanabe, S, Miyazaki, I, Asanuma, M, Kitamura, Y, Sendo, T

    2015.10.17 

     More details

    Event date: 2015.10.17 - 2015.10.21

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Doxorubicin and cyclophosphamide treatment causes anxiety-like behavior and spatial cognition impairment in rats.

    Kitamura, Y., Watanabe, S., Yoneda, S., Sugimoto, M., Kanemoto, E., Kanzaki, H., Machida, A., Miyazaki, I., Asanuma, M., Sendo, T.

    45th Annual Meeting of Society for Neuroscience (Neuroscience 2015)  2015.10.17 

     More details

    Event date: 2015.10.17 - 2015.10.21

    Language:English   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおけるアストロサイトの5-HT1Aレセプターを標的とした神経保護.

    宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 村上真樹, 北村佳久, 浅沼幹人

    第9回パーキンソン病・運動障害疾患コングレス  2015.10.15 

     More details

    Event date: 2015.10.15 - 2015.10.17

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ミルタザピンのアストロサイトを標的としたドパミン神経保護効果.

    宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 村上真樹, 北村佳久, 浅沼幹人

    第45回日本神経精神薬理学会 第37回日本生物学的精神医学会  2015.9.24 

     More details

    Event date: 2015.9.24 - 2015.9.26

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective profile of mirtazapine on astrocytes in parkinsonian mice.

    Miyazaki, I., Kikuoka, R., Kubota, N., Maeda, M., Kagawa, D., Murakami, S., Kitamura, Y., Asanuma, M.

    第56回日本神経学会学術大会  2015.5.20 

     More details

    Event date: 2015.5.20 - 2015.5.23

    Language:English   Presentation type:Poster presentation  

    researchmap

  • パパイア発酵食品のアストロサイトにおけるNrf2活性化作用.

    村上真樹, 宮崎育子, 浅沼幹人

    第88回日本薬理学会年会  2015.3.20 

     More details

    Event date: 2015.3.18 - 2015.3.20

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおけるミルタザピンの神経保護効果.

    宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 村上真樹, 北村佳久, 浅沼幹人

    第88回日本薬理学会年会  2015.3.19 

     More details

    Event date: 2015.3.18 - 2015.3.20

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Involvement of BDNF in doxorubicin and cyclophosphamide-induced spatial cognition in rats.

    Kitamura, Y, Yoneda, S, Miyazaki, I, Asanuma, M, Sendo, T

    44th Annual Meeting of Society for Neuroscience (Neuroscience 2014)  2014.11.15 

     More details

    Event date: 2014.11.15 - 2014.11.19

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ラットの行動変化から推察する抗がん剤投与による精神機能変化-ドキソルビシンおよびシクロホスファミド投与による影響-.

    北村佳久, 服部紗代, 米田紗緒里, 三宅彩香, 小山敏広, 宮崎育子, 浅沼幹人, 千堂年昭

    第26回日本サイコオンコロジー学会  2013.9.20 

     More details

    Event date: 2013.9.20 - 2013.9.21

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトの抗酸化因子の賦活機構と神経保護候補薬剤の探索

    宮崎育子, 浅沼幹人

    第53回日本神経学会総会  2012.5.22 

     More details

    Event date: 2012.5.22 - 2012.5.25

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 非シナプス性の神経伝達装置である神経細胞1次繊毛はドーパミン受容体を発現する.

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 浅沼幹人

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会  2011.10.27 

     More details

    Event date: 2011.10.27 - 2011.10.29

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アセチル-L-カルニチンの培養グリア細胞系への作用

    浦添夏帆, 村田麻衣子, 竹島美香, 宮崎育子, 浅沼幹人, 喜多大三

    第57回日本栄養改善学会学術総会  2010.9.10 

     More details

    Event date: 2010.9.10 - 2010.9.12

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 緑茶成分テアニンはアストログリアでのグルタチオン合成促進を介して参加ストレスによる神経細胞死を抑制する.

    竹島美香, 宮崎育子, 喜多大三, 浅沼幹人

    第57回日本栄養改善学会学術総会  2010.9.10 

     More details

    Event date: 2010.9.10 - 2010.9.12

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Influence of the suppression of cell proliferation and neurogenesis in the ability of antidepressants in an ACTH-induced animal model of treatment-resistant.

    Kitamura, Y, Doi, M, Hayashi, H, Miyazaki, I, Asanuma, M, Kawasaki, H

    The 16th World Congress of Basic and Clinical Pharmacology(World Pharma2011)  2010.7.17 

     More details

    Event date: 2010.7.17 - 2010.7.23

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Involvement of alpha7 nicotinic acetylcholine receptor on the conditioned place aversion induced by naloxone in single-dose morphine-treated rats..

    Ishida, S, Ukutam, T, Miyazaki, I, Asanuma, M, Matsunaga, H, Senndo, T, Araki, H, Kawasaki, H, Kitamura, Y

    The 16th World Congress of Basic and Clinical Pharmacology (World Pharma2010)  2010.7.17 

     More details

    Event date: 2010.7.17 - 2010.7.23

    Language:English   Presentation type:Poster presentation  

    researchmap

  • アストロサイトにおけるメタロチオネイン発現誘導によるドパミン神経保護

    宮崎育子, 吉川友理, 竹島 美香, 三好 耕, 浅沼幹人

    第52回日本神経化学会大会  2009.6.22 

     More details

    Event date: 2009.6.22 - 2009.6.24

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン神経毒性に対するインターフェロンγおよびPPARγアゴニストの保護効果

    浅沼幹人, 宮崎育子, 福岡早紀, 穂積宏彰, 辻武史, 北村佳久, 千堂年昭, 喜多大三

    第52回日本神経化学会大会  2009.6.22 

     More details

    Event date: 2009.6.22 - 2009.6.24

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Oxidative stress as measured by electron spin resonance in peritoneal effluents is a significant risk factor for peritoneal dialysis withdrawal and mortality.

    Morinaga, H, Sugiyama, H, Inoue, T, Takiue, K, Kimumoto, Y, Nakao, K, Maeshima, Y, Miyazaki, I, Hiramatsu, M, Aasanuma, M, Makino H

    45th European Renal Association-European dialysis and Transplant Association (ERA-EDTA) Congress  2008.5.10 

     More details

    Event date: 2008.5.10 - 2008.5.13

    Language:English   Presentation type:Poster presentation  

    researchmap

  • システイン基含有分子としてのメタロチオネインのドパミンキノン誘発神経障害に対する保護効果.

    浅沼幹人, 宮崎育子, 三好 耕, 穂積宏彰, 十川紀夫

    メタロチオネインおよびメタルバイオサイエンス研究会2007  2007.9.28 

     More details

    Event date: 2007.9.28 - 2007.9.29

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタロチオネイン欠損による脳梁形成不全の増悪

    正井加織, 菊岡 亮, 名越 武, 十川千春, 十川紀夫, 宮崎育子, 浅沼幹人

    日本解剖学会第75回中国・四国支部学術集会  2021.10.30 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロチゴチンによるアストロサイトを標的としたドパミン神経保護 Invited

    宮崎育子

    ニュープロパッチ 8周年記念講演会  2021.10.9 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    researchmap

  • 妊娠・授乳期エポキシ樹脂BADGE曝露による新生仔マウス脳発達異常におけるエストロゲンβレセプターの関与

    浅沼幹人, 宮崎育子, 西山千春, 菊岡亮, 名越 武, Kyle Quin, 禅正和真

    第43回日本生物学的精神医学会・第51回日本神経精神薬理学会合同年会  2021.7.16 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソン病モデルマウスにおける杜仲抽出物のメタロチオネイン発現誘導および神経保護効果

    今福史智, Jin Sun, 磯岡奈未, 上舞 直, 清水崇司, 豊田俊明, 岡本裕成, 菊岡 亮, 宮崎育子, 浅沼幹人

    第34回創薬・薬理フォーラム岡山  2020.12.26 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソン病モデルマウスにおける杜仲抽出物のメタロチオネイン発現誘導および神経保護効果

    今福史智, Jin Sun, 磯岡奈未, 上舞 直, 清水崇司, 豊田俊明, 岡本裕成, 菊岡 亮, 宮崎育子, 浅沼幹人

    メタルバイオサイエンス研究会2020  2020.11.6 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • アストロサイトのセロトニン1Aレセプターを標的としたドパミン神経保護

    磯岡奈未, 宮崎育子, 菊岡 亮, 和田晃一, 中山恵利香, 進 浩太郎, 山本大地, 北村佳久, 浅沼幹人

    第45回岡山脳研究セミナー  2020.1.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 妊娠・授乳期エポキシ樹脂 BADGE 曝露の新生仔マウス脳発達への影響

    浅沼幹人, 宮崎育子, 菊岡 亮, 磯岡奈未, 禅正和真, 新居 麗, 園部奏生, 船越英丸, 中山恵利香, 進 浩太郎, 山本大地, Kyle Quin

    第45回岡山脳研究セミナー  2020.1.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 抗うつ薬ミルタザピンの神経-アストロサイト連関を介したドパミン神経保護効果

    菊岡 亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 北村佳久, 浅沼幹人

    第45回岡山脳研究セミナー  2020.1.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロチゴチンのアストロサイトセロトニン1A受容体を標的としたド パミン神経保護効果

    磯岡 奈未, 宮崎 育子, 菊岡 亮, 和田 晃一, 中山 恵利香, 進 浩太郎, 山本 大地, 北村 佳久, 浅沼 幹人

    第32回創薬・薬理フォーラム  2019.12.21 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 神経毒によるドパミン神経障害における部位特異的神経-グリア連関

    浅沼幹人, 磯岡奈未, 菊岡亮, 宮崎育子

    第17回神経科学研究会  2019.11.23 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    researchmap

  • 抗うつ薬ミルタザピンの神経−グリア連関を介したドパミン神経保護作用

    菊岡 亮, 宮崎育子, 村上真樹, 北村佳久, 千堂年昭, 浅沼幹人

    第31回創薬・薬理フォーラム  2019.7.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロテノン誘発神経毒性に対するカフェイン酸,クロロゲン酸の神経保護効果

    磯岡奈未, 和田晃一, 古川智英子, 宮崎育子, 浅沼幹人

    第134回日本薬理学会近畿部会  2018.11.23 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 抗鬱薬ミルタザピンの神経ーグリア連関を介したドパミン神経保護効果

    菊岡 亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 村上真樹, 北村佳久, 浅沼幹人

    第134回日本薬理学会近畿部会  2018.11.23 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 抗うつ薬ミルタザピンによるアストロサイトの5-HT1A受容体を介したメタロチオネイン発現誘導とドパミン神経保護

    菊岡 亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 北村佳久, 浅沼幹人

    メタルバイオサイエンス研究会2018  2018.11.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおけるメタロチオネインを介したカフェイン酸,クロロゲン酸の神経保護効果

    磯岡奈未, 和田晃一, 古川智英子, 宮崎育子, 浅沼幹人

    メタルバイオサイエンス研究会2018  2018.11.16 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミン神経毒性に対するHMGB1抗体の保護効果

    黒田啓太, 磯岡奈未, 菊岡 亮, 村上真樹, 大熊 佑, 宮崎育子, 劉 克約, 西堀正洋, 浅沼幹人

    第29回創薬・薬理フォーラム  2018.7.14 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 磯岡奈未, 和田晃一, 宮崎育子, 菊岡 亮, 古川智英子, 浅沼幹人

    ロテノン誘発パーキンソン病モデルにおける腸管神経障害とコーヒー成分による神経保護効果

    第28回創薬薬理フォーラム岡山  2017.12.16 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロテノン誘発パーキンソン病モデルへのコーヒー成分カフェイン酸,クロロゲン酸投与による神経保護効果.

    磯岡奈未, 和田晃一, 宮崎育子, 菊岡 亮, 浅沼幹人, ロテノン誘発パーキンソン病モデルへのコーヒー成分カフェイン酸, クロロゲン酸投与による神経保護効果

    第27回創薬・薬理フォーラム岡山  2017.7.29 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 抗うつ薬ミルタザピンのアストロサイトにおけるメタロチオネイン発現誘導およびドパミン神経保護効果.

    菊岡 亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 北村佳久, 浅沼幹人

    第27回創薬・薬理フォーラム岡山  2017.7.29 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソン病での神経障害に対するコーヒー成分による腸内環境修飾の影響 Invited

    浅沼幹人, 宮崎育子

    全日本コーヒー協会平成28年度研究成果報告会  2017.6.16 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    researchmap

  • Influence of nicotine on doxorubicin and cyclophosphamide-induced spatial cognitive impairment and anxiety-like behavior in rats.

    Kitamura, Y, Sugimoto, M, Kanemoto, E, Machida, A, Nakamura, Y, Miyazaki, I, Asanuma, M, Sendo, T

    47th Annual Meeting of Society for Neuroscience (Neuroscience 2017)  2017 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおけるミルタザピンの神経保護効果.

    菊岡亮, 宮崎育子, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 村上真樹, 北村佳久, 浅沼幹人, 千堂年昭

    第27回霧島神経薬理フォーラム  2016.8.18 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 抗うつ薬ミルタザピンのアストロサイトにおけるメタロチオネイン発現誘導およびドパミン神経保護効果.

    宮崎育子, 菊岡亮, 久保田菜月, 前田恵実, 香川大樹, 守山雅晃, 粂 明日香, 村上真樹, 北村佳久, 浅沼幹人

    メタルバイオサイエンス研究会サテライト2016  2016.8.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルにおけるアストロサイトでのL-DOPA取り込み.

    宮崎育子, 村上真樹, 浅沼幹人

    第24回日本臨床精神神経薬理学会・第44回日本神経精神薬理学会合同年会,  2014.11.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミン欠乏による線条体ニューロンの1次繊毛の伸長.

    三好 耕, 松崎伸介, 宮崎育子, 浅沼幹人, 片山泰一

    第41回日本脳科学会  2014.11.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ALSモデルマウスにおけるセロトニン1Aアゴニストによる神経保護効果の検討.

    中野剛志, 鳥越菜央, 村上真樹, 宮崎育子, 浅沼幹人, 北村佳久, 千堂年昭

    第36回日本生物学的精神医学会 第57回日本神経化学会大会合同年会  2014.9.29 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトはL-DOPAのリザーバーとなりうる.

    宮崎育子, 村上真樹, 浅沼幹人

    第37回日本神経科学大会  2014.9.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Disc1遺伝子exon 6に欠損を持つマウスを用いたDisc1の解析.

    三好 耕, 笠原恭輔, 宮崎育子, 松崎伸介, 黒田啓介, 貝淵弘三, 浅沼幹人, 片山泰一

    第37回日本神経科学大会  2014.9.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン誘発パーキンソン病モデルマウスの中枢および腸管神経系におけるメタロチオネインの変化.

    村上真樹, 宮崎育子, 十川紀夫, 浅沼幹人

    第67回日本酸化ストレス学会学術集会  2014.9.4 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 農薬ロテノン誘発パーキンソン病モデルマウスにおける中枢および腸管神経障害とメタロチオネインによる神経保護.

    村上真樹, 宮崎育子, 十川紀夫, 浅沼幹人

    第21回創薬・薬理フォーラム  2014.7.26 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロテノン皮下投与による中枢・末梢神経系における経時的組織学的変化.

    村上真樹, 宮崎育子, 浅沼幹人

    第125回日本薬理学会近畿部会  2014.6.20 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • アストロサイトを介したレベチラセタムのドパミン神経保護効果に関する検討.

    宮崎育子, 村上真樹, 鳥越菜央, 北村佳久, 浅沼幹人

    第125回日本薬理学会近畿部会  2014.6.20 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソン病モデルマウスにおけるレベチラセタムの神経保護とアストロサイトの関与.

    宮崎育子, 村上真樹, 浅沼幹人

    第55回日本神経学会学術大会  2014.5.24 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおけるレベチラセタムによる神経保護効果.

    宮崎育子, 村上真樹, 鳥越菜央, 北村佳久, 浅沼幹人

    第87回日本薬理学会年会  2014.3.19 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 酸化ストレスに対するアストロサイトの神経保護作用における、脳部位特異的プロファイリング.

    鳥越菜央, 宮崎育子, 村上真樹, 北村佳久, 千堂年昭, 浅沼幹人

    第20回創薬・薬理フォーラム岡山  2013.12.21 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Comparative study of psychological response on treatment with doxorubicin and cyclophosphamide between rats and mice.

    Yoneda, S., Hattori, S., Nakamura, H., Watanabe, S., Koyama, T., Miyazaki, I., Asanuma, M., Kitamura, Y., Sendo, T.

    43rd Annual Meeting of Society for Neuroscience (Neuroscience2013)  2013.11.13 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Ketamine exerts antidepressant-like effects during the forced swim test in adrenocorticotropic hormone-treated rats.

    Nakamura, H, Yoneda, S, Miyake, A, Koyama, T, Miyazaki, I, Asanuma, M, Kitamura, Y, Sendo, T

    43rd Annual Meeting of Society for Neuroscience (Neuroscience2013)  2013.11.9 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Involvement of the 5-HT1A receptor function in the 8-OH-DPAT treatment on neurogenesis in ACTH-treated rats.

    Kitamura, Y., Miyake, A., Hattori, S., Koyama, T., Miyazaki, I., Asanuma M., Sendo, T.

    43rd Annual Meeting of Society for Neuroscience (Neuroscience2013)  2013.11.9 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • お茶の旨味成分テアニンの神経保護機構に関する研究 -培養アストログリア細胞におけるテアニンの細胞保護効果について-

    喜多大三, 浅沼幹人, 宮崎育子, 竹島美香

    第23回日本臨床神経精神薬理学会 第43回日本神経精神薬理学会 合同年会  2013.10.25 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • セロトニン1Aアゴニストによるアストロサイトにおけるメタロチオネイン発現誘導とドパミン神経保護.

    宮崎育子, 村上真樹, 竹島美香, 鳥越菜央, 三好 耕, 北村佳久, 浅沼幹人

    第23回日本臨床神経精神薬理学会 第43回日本神経精神薬理学会 合同年会  2013.10.25 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 環境毒誘発性パーキンソン病モデルマウスの中枢・末梢神経系障害におけるアストロサイトとメタロチオネインの関与.

    村上真樹, 宮崎育子, 鳥越菜央, 十川紀夫, 浅沼幹人

    第23回日本臨床神経精神薬理学会 第43回日本神経精神薬理学会 合同年会  2013.10.25 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 線条体アストロサイトが酸化ストレスに対して発現誘導する因子の網羅的解析.

    鳥越菜央, 宮崎育子, 村上真樹, 北村佳久, 千堂年昭, 浅沼幹人

    第23回日本臨床神経精神薬理学会 第43回日本神経精神薬理学会 合同年会  2013.10.25 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ニューロンの1次繊毛の解析.

    三好 耕, 松崎伸介, 宮崎育子, 浅沼幹人, 片山泰一

    第40回日本脳科学会  2013.9.28 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 創薬標的としてのアストロサイトのメタロチオネイン Invited

    浅沼幹人, 宮崎育子

    メタルバイオサイエンス研究会2013  2013.9.26 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • 抗がん剤投与動物における精神機能および神経新生に関する検討.

    米田紗緒里, 服部紗代, 中村紘子, 渡邊沙織, 村上真樹, 宮崎育子, 浅沼幹人, 北村佳久, 千堂年昭

    第24回霧島神経薬理フォーラム  2013.8.18 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Neuroprotective effects of serotoin 1A agonist target astrocytes.

    2013.7.5 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Astrocytes in the striatum act as a reservoir of L-DOPA but less convert to dopamine.

    Asanuma, M., Miyazaki, I.

    XI European Meeting on Glial Cells in Health and Disease  2013.7.3 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Mice carrying a 25-base-pair deletion in exon 6 of the Disc1 gene lack the Disc1 protein.

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 黒田啓介, 貝淵弘三, 片山泰一, 浅沼幹人

    第11回世界生物学的精神医学会国際会議 第35回日本生物学的精神医学会 合同大会  2013.6.23 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ロテノン神経毒性に対する中枢および末梢神経系のニューロンとグリアの変化とメタロチオネインの関与.

    村上真樹, 宮崎育子, 十川紀夫, 浅沼幹人

    第36回日本神経科学大会・第56回日本神経化学会大会・第23回日本神経回路学会大会合同大会  2013.6.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • セロトニン1Aアゴニストによる神経-アストロサイト連関の修飾

    宮崎育子, 村上真樹, 竹島美香, 鳥越菜央, 三好 耕, 北村佳久, 浅沼幹人

    第36回日本神経科学大会・第56回日本神経化学会大会・第23回日本神経回路学会大会合同大会  2013.6.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • セロトニン1Aアゴニストによるアストロサイトを標的としたドパミン神経保護.

    宮崎育子, 村上真樹, 竹島美香, 三好 耕, 浅沼幹人

    第54回日本神経学会学術大会  2013.5.29 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 中枢および末梢神経系におけるロテノンによる神経変性に対するメタロチオネインの関与.

    村上真樹, 宮崎育子, 鳥越菜央, 十川紀夫, 浅沼幹人

    第86回日本薬理学会年会  2013.3.23 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 5-HT1aアゴニスト8-OH-DPATによる神経保護効果はアストロサイトを標的とする.

    宮崎育子, 村上真樹, 竹島美香, 鳥越菜央, 三好 耕, 北村佳久, 浅沼幹人

    第86回日本薬理学会年会  2013.3.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドキソルビシンおよびシクロホスファミド投与ラットにおける精神機能変化および病態機序解明.

    北村佳久, 服部紗代, 三宅彩香, 小山敏広, 宮崎育子, 浅沼幹人

    第86回日本薬理学会年会  2013.3.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトの部位特異的プロファイリングと、その抗酸化防御機構を標的とした神経保護.

    鳥越菜央, 宮崎育子, 村上真樹, 小山敏広, 北村佳久, 浅沼幹人

    第86回日本薬理学会年会  2013.3.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ACTH反復投与ラットにおける5-HT1A受容体アゴニストの抗うつ効果および海馬神経新生に与える影響.

    三宅彩香, 北村佳久, 服部紗代, 小山敏広, 宮崎育子, 浅沼幹人, 千堂年昭

    第86回日本薬理学会年会  2013.3.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ACTH反復投与ラットにおける5-HT1A受 容体アゴニストの海馬神経新生および細胞内シグナリング伝達系に関する検討

    三宅彩香, 小山敏広, 宮崎育子, 浅沼幹人, 北村佳久

    第6回次世代を担う若手医療科学シンポジウム  2012.11.23 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • ドキソルビシン、シクロホスファミド処置ラットにおける精神障害の評価および解析

    服部紗代, 三宅彩香, 小山敏広, 宮崎育子, 浅沼幹人, 北村佳久

    第6回次世代を担う若手医療科学シンポジウム  2012.11.23 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • Rotenone-induced neurotoxicity in enteric and cerebral neuron-glia mixed culture

    宮崎育子, 村上真樹, 三好 耕, 浅沼幹人

    第55回日本神経化学会大会・第11回アジア太平洋神経化学会  2012.10.1 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Primary cilia and extra-synaptic neurotransmission

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 浅沼幹人

    第55回日本神経化学会大会・第11回アジア太平洋神経化学会  2012.9.30 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • マウスDisc1遺伝子exon 6の25塩基対の欠損はDisc1タンパクの発現を消失させる

    三好耕, 笠原恭輔, 村上真樹, 宮崎育子, 黒田啓介, 貝淵弘三, 片山泰一, 浅沼幹人

    第34回日本生物学的精神医学会  2012.9.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Disc1に25塩基対の欠損を持つマウスの解析

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 黒田啓介, 貝淵弘三, 片山泰一, 浅沼幹人

    第35回日本神経科学大会  2012.9.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルマウスにおける5-HT1aアゴニスト8-OH-DPATによる神経保護効果.

    宮崎育子, 村上真樹, 竹島美香, 浅沼幹人

    第85回日本薬理学会年会  2012.3.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 慢性ロテノン曝露の腸管神経叢ニューロンおよびグリアへの影響.

    村上真樹, 宮崎育子, 浅沼幹人

    第85回日本薬理学会年会  2012.3.15 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン暴露パーキンソン病モデルの腸管神経叢での神経障害とメタロチオネインの関与.

    村上真樹, 宮崎育子, 十川紀夫, 浅沼幹人

    メタロチオネインおよびメタルバイオサイエンス研究会2011  2011.12.8 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • アストロサイトの抗酸化機構を標的とした神経保護.

    浅沼幹人, 宮崎育子

    第9回神経科学研究会  2011.11.26 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ロテノン曝露パーキンソン病モデルの腸管神経叢における神経障害およびグリア細胞の関与.

    宮崎育子, 村上真樹, 竹島美香, 浅沼幹人

    第64回日本薬理学会西南部会  2011.11.20 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • L-テアニンのアストログリアでのグルタチオン増加を介したドパミン神経保護効果.

    竹島美香, 村上真樹, 宮崎育子, 浅沼幹人, 喜多大三

    第64回日本薬理学会西南部会  2011.11.20 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Effects of imipramine and lithium on the suppression of cell proliferation in the dentate gyrus of the hippocampus in ACTH-treated rats.

    Kuwatsuka, K, Onoue, Y, Hayashi, H, Doi, M, Koyama, T, Miyazaki, I, Asanuma, M, Kitamura, Y

    41th Annual Meeting of Society for Neuroscience  2011.11.16 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Uptake and metabolism of L-DOPA and dopamine in striatal astrocytes.

    Asanuma, M, Miyazaki, I, Murakami, S, Takeshima, M

    41th Annual Meeting of Society for Neuroscience  2011.11.16 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Effects of rotenone exposure on enteric neuronal or glial cells.

    Miyazaki, I, Murakami, S, Takeshima, M, Asanuma, M

    41th Annual Meeting of Society for Neuroscience  2011.11.16 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Effects of electroconvulsive stimuli on neurogenesis in dentate gyrus of ACTH-treated rats

    Koyama, T, Hayashi, H, Kuwatsuka, K, Onoue, Y, Doi, M, Miyazaki, I, Asanuma, M, Kitamura, Y

    41th Annual Meeting of Society for Neuroscience  2011.11.16 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ドーパミン伝達系とニューロンの1次繊毛の関係.

    笠原恭輔, 三好 耕, 村上真樹, 宮崎育子, 浅沼幹人

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会  2011.10.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 神経疾患とアストロサイトの抗酸化機構 Invited

    宮崎育子

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会合同年会  2011.10.27 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • アストロサイトに取り込まれたL-DOPAおよびドパミンの代謝に関する検討.

    浅沼幹人, 村上真樹, 竹島美香, 三好 耕, 宮崎育子

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会  2011.10.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 培養グリア細胞系におけるメタンフェタミンによる細胞毒性発現.

    喜多大三, 竹島美香, 三島顕人, 宮崎育子, 浅沼幹人

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会  2011.10.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン曝露の腸管神経叢における神経およびグリア細胞への影響.

    浅沼幹人, 村上真樹, 宮崎育子

    第5回パーキンソン病・運動障害疾患コングレス  2011.10.7 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 線条体アストロサイトに取り込まれたL-DOPAの利用効率.

    宮崎育子, 村上真樹, 浅沼幹人

    第54回日本神経化学会大会  2011.9.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • L-DOPAの初代培養ドパミン神経保護効果およびそれに対する3-OMDの抑制作用はアストロサイトを標的としている.

    浅沼幹人, 村上真樹, 宮崎育子

    第54回日本神経化学会大会  2011.9.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドーパミン受容体は非シナプス性の神経伝達装置である神経細胞1次繊毛に局在する.

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 浅沼幹人

    第34回日本神経科学大会  2011.9.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 腸管神経叢ニューロンおよびグリアに対するロテノン暴露の影響.

    宮崎育子,村上真樹,竹島美香,三好 耕,浅沼幹人

    第34回日本神経科学大会  2011.9.15 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン慢性投与パーキンソン病モデルにおける腸管神経叢ニューロンおよびグリアの変化.

    村上真樹, 宮崎育子, 竹島美香, 三好 耕, 浅沼幹人

    第34回日本神経科学大会  2011.9.15 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドーパミン伝達がニューロンの1次繊毛に及ぼす影響.

    笠原恭輔, 三好 耕, 村上真樹, 宮崎育子, 浅沼幹人

    第33回日本生物学的精神医学会  2011.5.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 非シナプス性の神経伝達を1次繊毛が媒介する可能性について.

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 浅沼幹人

    第33回日本生物学的精神医学会  2011.5.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • L-DOPAによるドパミン神経細胞増殖作用と3-OMDの抑制効果におけるアストロサイトの関与.

    浅沼幹人, 竹島美香, 村上真樹, 三好 耕, 宮崎育子

    第52回日本神経学会学術大会  2011.5.20 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ACTH反復投与ラットにおける海馬細胞新生の減少およびそのメカニズムに関する検討.

    林 宏美, 土居真穂, 尾上由華, 鍬塚圭子, 三宅綾香, 小山敏広, 四宮一昭, 宮崎育子, 浅沼幹人, 北村佳久

    日本薬学会第131年会,学生シンポジウム 創薬を目指す若手薬学研究者の挑戦 -病態モデルと薬効評価による治療戦略への薬理学的展開-  2011.3.29 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • ACTH反復投与ラットにおける海馬細胞新生の減少に対するimipramine、lithiumの作用に関する検討.

    林 宏美, 土居真穂, 尾上由華, 鍬塚圭子, 小山敏広, 四宮一昭, 宮崎育子, 浅沼幹人, 北村佳久

    第84回日本薬理学会年会  2011.3.24 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミンによるグリア細胞毒性発現とアセチル-L-カルニチンの細胞保護効果について.

    浦添夏帆, 村田麻衣子, 竹島美香, 宮崎育子, 浅沼幹人, 喜多大三

    第63回日本薬理学会西南部会・第20回日韓薬理学会合同セミナー  2010.11.26 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミンが神経細胞一次繊毛に及ぼす影響.

    笠原恭輔, 三好 耕, 宮崎育子, 浅沼幹人

    第32回日本生物学的精神医学会  2010.10.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 繊毛が媒介する非シナプス性の神経伝達と精神疾患.

    三好 耕, 笠原恭輔, 村上真樹, 宮崎育子, 浅沼幹人

    第32回日本生物学的精神医学会  2010.10.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • フェニルアルキルアミン系違法ドラッグによるモノアミン神経毒性に関する検討.

    宮崎育子, 吉川友理, 竹島美香, 三好 耕, 船田正彦, 浅沼幹人

    第20回日本臨床精神神経薬理学会・第40回日本神経精神薬理学会合同年会  2010.9.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 培養グリア細胞系におけるメタンフェタミン細胞毒性に対するアセチル-L-カルニチンの作用

    浦添夏帆, 村田麻衣子, 竹島美香, 宮崎育子, 浅沼幹人, 喜多大三

    第20回日本臨床精神神経薬理学会・第40回日本神経精神薬理学会合同年会  2010.9.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 緑茶成分テアニンのアストロサイトでの抗酸化機構の賦活作用とドパミン神経保護効果.

    竹島美香, 宮崎育子, 吉川友理, 村上真樹, 喜多大三, 浅沼幹人

    第20回日本臨床精神神経薬理学会・第40回日本神経精神薬理学会合同年会  2010.9.15 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトによるドパミンキノン毒性に対する神経保護.

    宮崎育子, 吉川友理, 竹島美香, 三好 耕, 喜多大三, 浅沼幹人

    第33回日本神経科学大会 第53回日本神経化学会大会 第20回日本神経回路学会大会合同大会  2010.9.4 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • リチウムは神経細胞1次繊毛を伸長する.

    三好 耕, 笠原恭輔, 宮崎育子, 浅沼幹人

    第33回日本神経科学大会 第53回日本神経化学会大会 第20回日本神経回路学会大会合同大会  2010.9.3 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 線条体アストロサイトにおけるL-DOPAおよびドパミンの取り込みと代謝.

    浅沼幹人, 宮崎育子

    第51回日本神経学会総会  2010.5.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ACTH反復投与ラットを用いた海馬歯状回における細胞増殖およびアストログリア活性に及ぼす影響

    土居真穂, 長町智子, 江川真希, 宮崎育子, 川崎博己, 千堂年昭, 浅沼幹人, 北村佳久

    第83回日本薬理学会年会  2010.3.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Morphine単回投与ラットにおけるnaloxone誘導条件付け場所嫌悪行動およびc-Fos発現に対するニコチン受容体の関与

    石田 茂, 河崎陽一, 浅沼幹人, 松永 尚, 千堂年昭, 荒木博陽, 川崎博己, 北村佳久

    第83回日本薬理学会年会  2010.3.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 培養ドパミン神経系におけるメタンフェタミン神経毒性に対するドコサヘキサエン酸の作用

    村田麻衣子, 浦添夏帆, 宮崎育子, 浅沼幹人, 喜多大三

    第83回日本薬理学会年会  2010.3.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 活性化アストロサイトにおけるドパミントランスポーターを介したドパミン特異的メ タロチオネイン誘導

    宮崎育子, 吉川友理, 竹島美香, 浅沼幹人

    第83回日本薬理学会年会  2010.3.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • L-テアニンはアストロサイトのグルタチオンを増加させ,過剰ドパミンによる神経細 胞死を抑制する

    竹島美香, 宮崎育子, 吉川友理, 喜多大三, 浅沼幹人

    第83回日本薬理学会年会  2010.3.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • フィチン酸の培養ドパミン細胞系への作用

    浦添夏帆, 村田麻衣子, 竹島美香, 吉川友理, 宮崎育子, 浅沼幹人, 喜多大三

    第62回日本薬理学会 西南部会  2009.11.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 培養ドパミン細胞系におけるフィチン酸の効果

    浦添夏帆, 村田麻衣子, 竹島美香, 吉川友理, 宮崎育子, 浅沼幹人, 喜多大三

    第19回日本臨床精神神経薬理学会・第39回日本神経精神薬理学会 合同年会  2009.11.14 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Effects of psychotropic drugs and pericentrin mutation on neuronal primary cilia

    Miyoshi, K, Kasahara, K, Miyazaki, I, Asanuma, M

    第19回日本臨床精神神経薬理学会・第39回日本神経精神薬理学会 合同年会  2009.11.14 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ドパミンキノン誘発神経障害に対するバイカレインの保護効果

    竹島美香, 宮崎育子, 村田麻衣子, 吉川友理, 浦添夏帆, 喜多大三, 浅沼幹人

    第19回日本臨床精神神経薬理学会・第39回日本神経精神薬理学会 合同年会  2009.11.14 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Effects of imipramine and lithium on the suppression of cell proliferation and neurogenesis of dentate gyrus of hippocampus in ACTH-treated rats

    Doi, M, Miyazaki, I, Shinomiya, K, Asanuma, M, Kitamura, Y

    第19回日本臨床精神神経薬理学会・第39回日本神経精神薬理学会 合同年会  2009.11.14 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • マウス脳の神経細胞1次繊毛に対するメタンフェタミンの作用

    笠原恭輔, 三好 耕, 宮崎育子, 浅沼幹人

    第19回日本臨床精神神経薬理学会・第39回日本神経精神薬理学会 合同年会  2009.11.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • フラボノイド,バイカレインの抗酸化能に関する検討

    竹島美香, 宮崎育子, 村田麻衣子, 吉川友理, 浦添夏帆, 喜多大三, 浅沼幹人

    第116回日本薬理学会 近畿部会  2009.11.13 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 酸化ストレスに対するアストロサイトでのメタロチオネイン発現はドパミン神経保護的に作用する

    宮崎育子, 浅沼幹人

    メタロチオネインおよびメタルバイオサイエンス研究会  2009.10.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトでの抗酸化機構の重要性と新規パーキンソン病治療薬ゾニサミドの神経保護効果

    浅沼幹人, 宮崎育子

    第7回神経化学研究会  2009.9.19 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミン神経毒性におけるアストロサイトでのメタロチオネイン発現誘導

    宮崎育子, 吉川友理, 竹島美香, 三好 耕, 喜多大三, 浅沼幹人

    第32回日本神経科学大会  2009.9.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 129系由来のDisc1欠損変異を持つマウスの解析

    三好 耕, 笠原恭輔, 宮崎育子, 浅沼幹人

    第32回日本神経科学大会  2009.9.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective properties of astrocytes through induction of quinone-quenching molecules in parkinsonian model

    Miyazaki, I, Kikkawa, Y, Takeshima, M, Miyoshi, K, Asanuma, M.

    22th Biennial Meeting of the International Society for Neurochemistry (ISN)/the Asian-Pacific Society for Neurochemistry (APSN) Joint Meeting  2009.8.25 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Reduction of PPAR-g in methamphetamine-induced neurotoxicity and protective effects of interferon-g

    Asanuma, M, Miyazaki, I, Kikkawa, Y, Takeshima, M, Miyoshi, K, Kita, T

    22th Biennial Meeting of the International Society for Neurochemistry (ISN)/the Asian-Pacific Society for Neurochemistry (APSN) Joint Meeting  2009.8.24 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • L-DOPA treatment-specific induction of metallothionein in reactive astrocytes in the striatum of parkinsonian model and its neuroprotective effects against dopaminergic neurotoxicity

    Miyazaki, I, Asanuma, M

    13th International Congress of Parkinson’s Disease and Movement Disorders  2009.6.10 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • A novel anti-parkinsonian agent zonisamide has astrocyte-proliferating effect and neuroprotective effect against 6-OHDA-induced dopaminergic neurodegeneration

    Asanuma, M, Miyazaki, I

    13th International Congress of Parkinson’s Disease and Movement Disorders  2009.6.8 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルのアストロサイトでのキノン消去分子の誘導を介した抗酸化機構

    浅沼幹人, 宮崎育子

    第50回日本神経学会総会  2009.5.20 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 過剰ドパミン誘発神経障害に対するアストロサイトでの抗酸化機構の重要性

    宮崎育子, 浅沼幹人

    第17回カテコールアミンと神経疾患研究会  2009.4.18 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ドコヘキサエン酸の培養ドパミン神経系への作用

    村田麻衣子, 竹島美香, 染矢 恵, 宮崎育子, 浅沼幹人, 喜多大三

    第82回日本薬理学会年会  2009.3.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • L-テアニンの培養ドパミン神経系への作用

    染矢 恵, 竹島美香, 村田麻衣子, 宮崎育子, 浅沼幹人, 喜多大三

    第82回日本薬理学会年会  2009.3.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン神経毒性におけるPRAR-γの関与とインターフェロン-γの保護効果

    福岡早紀, 穂積宏彰, 木本直孝, 吉川友理, 辻 武史, 宮崎育子, 北村佳久, 千堂年昭, 喜多大三, 浅沼幹人

    第82回日本薬理学会年会  2009.3.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノン暴露後早期のアストロサイトの反応

    木本直孝, 福岡早紀, 吉川友理, Diaz-Corrales, Francisco J, 宮崎育子, 浅沼幹人

    第82回日本薬理学会年会  2009.3.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ACTH反復投与ラットにおける中枢ドパミントランスポーター発現に関する検討

    江本清香, 北村佳久, 宮崎育子, 北川航平, 長町智子, 土居真穂, 石丸由衣, 浅沼幹人, 千堂年昭

    第82回日本薬理学会年会  2009.3.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • フラボノイド,バイカレインの培養ドパミン神経系への作用

    竹島美香, 染矢 恵, 村田麻衣子, 宮崎育子, 浅沼幹人, 喜多大三

    第61回日本薬理学会西南部会  2008.11.28 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Development of animal model of treatment-resistant-depression in rats -Effects of antidepressants on the duration of immobility of ACTH-treated rats in the forced swim test.

    Kitamura, Y, Kitagawa, K, Miyazaki, T, Nagamachi, T, Doi, M, Miyazaki, I, Asanuma, M, Sendo, T, Kawasaki, H, Gomita,Y

    38th Annual Meeting of Society for Neuroscience  2008.11.16 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ドパミンキノン障害性に対するアスピリンの保護効果

    吉川友理, 木本直孝, 福岡早紀, 宮崎育子, 浅沼幹人

    第114回日本薬理学会近畿部会  2008.11.14 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • テアニンの培養モノアミン神経およびグリア細胞系への作用

    染矢 恵, 村田麻衣子, 竹島美香, 宮崎育子, 浅沼幹人, 喜多大三

    第18回日本臨床精神神経薬理学会・第38回日本神経精神薬理学会合同大会  2008.10.2 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • グリアでのメタロチオネイン発現誘導によるドパミン神経保護効果

    宮崎育子, 吉川友理, 木本直孝, 三好 耕, 浅沼幹人

    第18回日本臨床精神神経薬理学会・第38回日本神経精神薬理学会合同大会  2008.10.1 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 神経細胞1次繊毛は情動や摂食に関与する

    三好 耕, 笠原恭輔, 宮崎育子, 浅沼幹人

    第2回アジア・太平洋生物学的精神医学会・第30回日本生物学的精神医学会  2008.9.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 神経細胞1次繊毛の精神疾患への関与

    三好 耕, 笠原恭輔, 宮崎育子, 浅沼幹人

    第51回日本神経化学会大会  2008.9.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • L-DOPA投与に特異的な活性化アストロサイトにおけるメタロチオネインの発現誘導

    宮崎育子, 吉川友理, 木本直孝, 三好 耕, 浅沼幹人

    第51回日本神経化学会大会  2008.9.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • マウス脳の神経細胞1次繊毛に対する向精神薬の作用

    笠原恭輔, 三好 耕, 宮崎育子, 浅沼幹人

    第2回アジア・太平洋生物学的精神医学会・第30回日本生物学的精神医学会  2008.9.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン神経毒性に対するインターフェロンγの保護効果におけるPPARγの関与

    浅沼幹人, 福岡早紀, 穂積宏彰, 宮崎育子, 北村佳久, 千堂年昭, 喜多大三

    第51回日本神経化学会大会  2008.9.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Pericentrin変異マウスを用いた神経細胞繊毛の機能解析

    三好 耕, 笠原恭輔, 宮崎育子, 浅沼幹人

    第31回日本神経科学大会  2008.7.10 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • セフトリアキソンの脳内グルタミン酸トランスポーターGLT-1発現誘導に関する検討

    吉川友理, 木本直孝, 福岡早紀, 宮崎育子, 浅沼幹人

    第113回日本薬理学会近畿部会  2008.6.20 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミン急性神経毒性に対するインターフェロン-γの保護効果発現機序に関する検討

    福岡早紀, 浅沼幹人, 宮崎育子, 穂積宏彰, 吉川友理, 木本直孝, 北村佳久, 千堂年昭, 喜多大三

    第113回日本薬理学会近畿部会  2008.6.20 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミン急性神経毒性に対するIFN-γの保護効果

    穂積宏彰, 浅沼幹人, 宮崎育子, 福岡早紀, 吉川友理, 木本直孝, 北村佳久, 千堂年昭, 喜多大三, 五味田 裕

    第81回日本薬理学会年会  2008.3.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • フラボン配糖体バイカリンの培養ドパミン神経系への作用

    竹島美香, 田中弓子, 染矢 恵, 村田麻衣子, 宮崎育子, 浅沼幹人, 喜多大三

    第81回日本薬理学会年会  2008.3.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ラット海馬神経細胞及びアストロサイトの形態学的変化に対するACTH反復投与の影響

    長町智子, 宮崎育子, 江本清香, 土居真穂, 川崎博巳, 浅沼幹人, 北村佳久, 千堂年昭, 五味田 裕

    第81回日本薬理学会年会  2008.3.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン急性神経毒性に対するインターフェロン-γの保護効果.

    穂積宏彰, 浅沼幹人, 宮崎育子, 福岡早紀, 吉川友理, 木本直孝, 北村佳久

    第8回創薬薬理フォーラム  2007.12.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 培養モノアミン神経系に及ぼすベスフェノールAの毒性に関する検討.

    竹島美香, 田中弓子, 宮崎育子, 浅沼幹人, 喜多大三

    第60回日本薬理学会西南部会  2007.11.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Protective effects of a novel anti-parkinsonian agent zonisamide on dopamine quinone-related neurotoxicity.

    Asanuma, M, Miyazaki, I

    37th Annual Meeting of Society for Neuroscience  2007.11.4 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Glutathione-increasing mechanism of a novel anti-parkinsonian agent zonisamide in the basal ganglia.

    Miyazaki, I, Asanuma, M

    37th Annual Meeting of Society for Neuroscience  2007.11.4 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 違法ドラッグ(いわゆる脱法ドラッグ)の精神依存性および神経毒性:フェネチルアミン誘導体の評価.

    舩田正彦, 青尾直也, 浅沼幹人, 宮崎育子, 花尻(木倉)瑠理, 合田幸広, 和田 清

    第42回日本アルコール・薬物医学会総会  2007.9.28 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソン病モデルの線条体グリア細胞でのグルタチオン合成.

    浅沼幹人, 宮崎育子, 穂積宏彰

    第5回神経科学研究会  2007.9.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 培養モノアミン神経系に及ぼすビスフェノールAの作用.

    竹島美香, 田中弓子, 宮崎育子, 浅沼幹人, 喜多大三

    第54回日本栄養改善学会  2007.9.20 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンキノン神経毒性におけるグルタチオン合成関連分子のグリアでの変化.

    浅沼幹人, 宮崎育子, 三好 耕, 穂積宏彰, 十川紀夫

    Neuroscience 2007(第30回日本神経科学大会・第50回日本神経化学会大会・ 第17回日本神経回路学会大会 合同学会)  2007.9.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンキノン誘発神経障害に対するメタロチオネインの保護効果.

    宮崎育子, 穂積宏彰, 三好 耕, 浅沼幹人

    Neuroscience 2007(第30回日本神経科学大会・第50回日本神経化学会大会・ 第17回日本神経回路学会大会 合同学会)  2007.9.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Pericentrin変異マウスを用いた神経細胞1次繊毛の機能解析.

    三好 耕, 宮崎育子, 浅沼幹人

    Neuroscience 2007(第30回日本神経科学大会・第50回日本神経化学会大会・第17回日本神経回路学会大会 合同学会)  2007.9.10 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 神経細胞1次繊毛の生物学的意義の検討.

    三好 耕, 宮崎育子, 浅沼幹人

    第29回日本生物学的精神医学会,第37回日本神経精神薬理学会  2007.7.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンキノン神経毒性におけるグリアでのグルタチオン合成関連分子の変化.

    宮崎育子, 穂積宏彰, 三好 耕, 浅沼幹人

    第29回日本生物学的精神医学会,第37回日本神経精神薬理学会  2007.7.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ビスフェノールAの培養モノアミン神経系への作用.

    竹島美香, 田中弓子, 宮崎育子, 浅沼幹人, 喜多大三

    第29回日本生物学的精神医学会,第37回日本神経精神薬理学会  2007.7.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン神経毒性に関与する炎症・免疫関連分子の網羅的腱索.

    穂積宏彰, 宮崎育子, 喜多大三, 北村佳久, 千堂年昭, 五味田裕, 浅沼幹人

    第29回日本生物学的精神医学会,第37回日本神経精神薬理学会  2007.7.12 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミン神経特異的酸化ストレスとしてのドパミンキノン神経障害に対するメタロチオネインの作用.

    宮崎育子, 浅沼幹人

    第34回日本脳科学会  2007.6.8 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • p53関連因子PAG608のL-DOPA投与パーキンソン病モデルの運動ニューロンでの特異的発現.

    浅沼幹人, 宮崎育子

    第34回日本脳科学会  2007.6.8 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソン病モデル線条体でのL-DOPA誘発キノン体生成とシステイン基含有分子.

    浅沼幹人, 宮崎育子, 穂積宏彰, 十川紀夫

    第48回日本神経学会総会  2007.5.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メチルフェニデートのドパミン神経毒性に対する作用.

    日名俊行, 浅沼幹人, 喜多大三, 宮崎育子, 小川紀雄, 北村佳久, 千堂年昭, 五味田裕

    日本薬学会第127年会  2007.3.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Protective effects of a novel anti-parkinsonian agent zonisamide on dopamine quinone-related neurotoxicity.

    Miyazaki, I, Asanuma, M, Diaz-Corrales, F.J, Ogawa, N

    10th International Congress of Parkinson's Disease and Movement Disorders  2006.10.31 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • A novel anti-parkinsonian agent zonisamide increases glutathione levels in the basal ganglia.

    Asanuma, M, Miyazaki, I, Diaz-Corrales, F.J, Ogawa, N

    10th International Congress of Parkinson's Disease and Movement Disorders  2006.10.31 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン急性神経毒性におけるドパミンキノン体生成の関与とキノン消去による阻止 Invited

    浅沼幹人, 宮崎育子, 北市清幸

    第18回日本アルコール精神医学会・第9回ニコチン・薬物依存研究フォーラム平成18年度合同学術総会  2006.9.29 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • Effect of methylphenidate on excess extra-vesicular dopamine-induced dopaminergic neurotoxicity.

    浅沼幹人, 喜多大三, 日名俊行, 宮崎育子, 小川紀雄, 北村佳久, 千堂年昭, 五味田裕

    第28回日本生物学的精神医学会・第36回日本神経精神薬理学会・第49回日本神経化学会合同年会  2006.9.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Dopamine quinone-related neurotoxicity and potential neuroprotective agents.

    宮崎育子, 浅沼幹人, 小川紀雄

    第28回日本生物学的精神医学会・第36回日本神経精神薬理学会・第49回日本神経化学会合同年会  2006.9.15 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Involvement of pericentrin in the formation of neuronal primary cilia.

    Miyoshi K, Miyazaki, I, Asanuma, M

    第28回日本生物学的精神医学会・第36回日本神経精神薬理学会・第49回日本神経化学会合同年会  2006.9.14 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Pericentrinは発達期大脳皮質の神経一次繊毛の基部に局在する.

    三好 耕, 宮崎育子, 浅沼幹人

    第29回日本神経科学大会  2006.7.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アポトーシス促進因子PAG608のL-DOPA投与パーキンソン病モデル脊髄運動ニューロンでの特異的発現.

    宮崎育子, 清水雅子, Francisco, J. Diaz-Correles, Maria J. Esrava-Alva, 浅沼幹人

    第29回日本神経科学大会  2006.7.20 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • シナプス小胞外遊離ドパミンからのドパミンキノン障害性とその消去による神経保護効果.

    浅沼幹人, 宮崎育子, 小川紀雄

    第33回日本脳科学会  2006.6.2 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ドパミン神経特異的酸化ストレスとしてのドパミンキノンに対するドパミンアゴニストの作用.

    宮崎育子, 浅沼幹人, 小川紀雄

    第33回日本脳科学会  2006.6.2 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Mechanism of protective effect of non-steroidal anti-inflammatory drugs on methamphetamine-induced neurotoxicity.

    宮崎育子, 辻 武史, 浅沼幹人, Francisco J, Díaz-Corrales, 三好 耕, 小川紀雄

    第48回日本神経化学会  2005.9.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Transplantation of tyrosinase cDNA-transfected hepatocytes into the atriatum of hemi-parkinsonian model.

    浅沼幹人, 宮崎育子, Francisco J, Díaz-Corrales, 難波正義, 小川紀I雄

    第48回日本神経化学会  2005.9.28 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • p53関連アポトーシス促進因子PAG608のパーキンソン病モデル線条体でのL-DOPA投与による特異的誘導と脳神経核運動ニューロンでの特異的局在.

    浅沼幹人, 宮崎育子, 清水雅子, 小川紀雄

    第3回神経科学研究会  2005.9.17 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミン神経毒性発現におけるキノン生成の関与.

    宮崎育子, 浅沼幹人, Francisco, J. Diaz-Corrales, 清水雅子, 三好 耕, 小川紀雄

    第28回日本神経科学大会  2005.7.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 発達期マウスにおけるpericentrinの発現解析.

    三好 耕, 大西一成, 浅沼幹人, 宮崎育子, Francisco, J. Diaz-Corrales, 小川紀I雄

    第28回日本神経科学大会  2005.7.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデルへのL-DOPA投与により誘発されるアポトーシス促進因子PAG608の運動ニューロンでの特異的発現.

    浅沼幹人, 清水雅子, 宮崎育子, Francisco J, Díaz-Corrales,三好耕, 小川紀I雄

    第28回日本神経科学大会  2005.7.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Aggregation of proteins in the centrosome and its importance in the neurodegenerative process.

    Diaz-Corrales FJ, Asanuma M, Miyazaki I, Miyoshi K, Ogawa N

    第27回日本生物学的精神医学会・第35回日本神経精神薬理学会合同年会  2005.7.8 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • キノン生成体のメタンフェタミン急性ドパミン神経毒性発現における関与.

    宮崎育子, 浅沼幹人, Diaz-Corrales FJ, 三好 耕, 清水雅子, 北市清幸, 小川紀雄

    第27回日本生物学的精神医学会・第35回日本神経精神薬理学会合同年会  2005.7.7 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミン神経特異的酸化ストレスとしてのドパミンキノンに対するシステイン基含有分子の消去作用.

    浅沼幹人, 宮崎育子, 小川紀雄

    第32回日本脳科学会  2005.6.3 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 過剰ドパミンおよびメタンフェタミン誘発ドパミン神経障害における共通因子としてのキノン体生成.

    宮崎育子, 浅沼幹人, 小川紀雄

    第32回日本脳科学会  2005.6.3 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • パーキンソ病モデル線条体に特異的なL-DOPA誘発キノン体生成.

    浅沼幹人, 宮崎育子, 小川紀雄

    第46回日本神経学会総会  2005.5.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ロテノンのドパミン神経毒性における中心体-微小管構成異常の関与.

    Francisco J. Diaz-Corrales, 浅沼幹人, 宮崎育子, 三好 耕, 小川紀雄

    第13回カテコールアミンと神経疾患研究会  2005.4.23 

     More details

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • Dopamine agonist pergolide prevents levodopa-induced quinoprotein formation in parkisonian striatum and shows quenching effects on dopamine-semiquinone generated in vitro.

    Ogawa, N, Asanuma, M, Miyazaki, I, Díaz-Corrales, F.J

    9th International Congress of Parkinson's Disease and Movement Disorders,  2005.3.7 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Transplantation of tyrosinase-producing cells into parkinsonian model

    Asanuma, M, Miyazaki, I, Ogawa, N

    34th Annual Meeting of Society for Neuroscience  2004.10.26 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • DISC1は中心体タンパクkendrinと結合する

    三好 耕, 大西一成, 浅沼幹人, 宮崎育子, Francisco Diaz, 小川紀雄

    第47回日本神経化学大会・第27回日本神経科学大会  2004.9.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 5MeO-DIPTによるドパミン神経細胞毒性

    宮崎育子, 浅沼幹人, 小川紀雄, 舩田正彦

    第47回日本神経化学大会・第27回日本神経科学大会  2004.9.22 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • マンガン誘発ドパミン神経毒性とパーキンの保護効果におけるキノン体生成の関与

    浅沼幹人, 宮崎育子, 小川紀雄

    第47回日本神経化学大会・第27回日本神経科学大会  2004.9.21 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 統合失調症・気分障害候補遺伝子DISC1産物は中心体タンパクkendrinと結合する

    三好 耕, 大西一成, 浅沼幹人, 宮崎育子, Francisco Diaz, 小川紀雄

    第34回日本神経精神薬理学会・第26回日本生物学的精神医学会合同年会  2004.7.23 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 5MeO-DIPTのドパミン神経毒性に関する検討

    宮崎育子, 浅沼幹人, 三好 耕, 小川紀雄, 舩田正彦

    第34回日本神経精神薬理学会・第26回日本生物学的精神医学会合同年会  2004.7.23 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • マンガン誘発神経毒性に対するパーキンの保護効果とドパミンの関与

    宮崎育子, 浅沼幹人, 東 洋一郎, 小川紀雄

    第31回日本脳科学会  2004.6.11 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミンのドパミン神経毒性におけるチロシナーゼの関与

    浅沼幹人, 宮崎育子, 小川紀雄

    第31回日本脳科学会  2004.6.11 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • マンガン誘発ドパミン神経毒性に対するパーキンの保護効果とドパミンキノンの関与

    浅沼幹人, 宮崎育子, 小川紀雄

    第45回日本神経学会総会  2004.5.13 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタロチオネインとパーキンソン病 Invited

    浅沼幹人, 宮崎育子, 小川紀雄

    メタロチオネイン2003  2003.11.22 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • メタロチオネインは神経毒6-hydroxydopamine 毒性を減弱させるか

    宮崎育子, 浅沼幹人, 小川紀雄

    第41回メタロチオネイン研究会  2003.11.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • マンガン誘発神経毒性におけるパーキン蛋白のドパミン神経特異的保護効果

    浅沼幹人, 宮崎育子

    第1回神経科学研究会  2003.10.25 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • マンガン誘発神経毒性におけるパーキン蛋白のドパミン神経特異的な関与

    宮崎育子, 浅沼幹人, 東 洋一郎, 服部信孝, 水野美邦, 小川紀雄

    第33回日本神経精神薬理学会年会  2003.10.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Rotenone induces fragmentation of Golgi apparatus in dopaminergic neuroblastoma cell line.

    ディアス フランシスコ, 浅沼幹人, 宮崎育子, 小川紀雄

    第33回日本神経精神薬理学会年会  2003.10.9 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • メタフェタミンによるドパミン神経障害に対する非ステロイド性消炎鎮痛薬の効果 -II-

    辻 武史, 浅沼幹人, 宮崎育子, 小川紀雄

    第33回日本神経精神薬理学会年会  2003.10.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 線条体アストロサイトにおけるドパミンレセプターの発現

    宮崎育子, 浅沼幹人, 小川紀雄

    第46回日本神経化学会大会  2003.9.25 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデル線条体へのメラニン合成酵素チロシナーゼ産生細胞の移植.

    浅沼幹人, 宮崎育子, 小川紀雄

    第46回日本神経化学会大会  2003.9.24 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Specific gene expression and possible involvement of inflammation in methamphetamine-induced neurotoxicity.

    Asanuma, M, Miyazaki, I, Higashi, Y, Tsuji, T, Ogawa, N

    ISN /APSN Sponsored Satellite Meeting. Current Status of Dependence/Abuse Studies: Cellular and Molecular Mechanisms of Drugs of Abuse and Neurotoxicity  2003.8.1 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Involvement of parkin protein in manganese-induced ER stress in dopaminergic cells.

    浅沼幹人, 東 洋一郎, 宮崎育子, 服部信孝, 水野美邦, 小川紀雄

    第26回日本神経科学大会  2003.7.24 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Possible involvement of tyrosinase in methamphetamine-induced dopaminergic toxicity.

    宮崎育子, Haque Md. Emdadul, 浅沼幹人, 小川紀雄

    第26回日本神経科学大会  2003.7.23 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンおよびその誘導体のキノン体生成を介したアポトーシス誘導性.

    浅沼幹人, 宮崎育子, M. Emdadul Haque, 東 洋一郎, 小川紀雄

    第11回カテコールアミンと神経疾患研究会  2003.4.19 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メタンフェタミンによるドパミン神経毒性に関与するPAG608の作用機構の解析と非ステロイド性消炎鎮痛薬投与の効果に関する検討

    浅沼幹人, 宮崎育子, 東 洋一郎, 辻 武史, 小川紀雄

    厚生科学研究費補助金医薬安全総合研究事業「規制薬物の依存及び神経毒性の発現にかかわる仕組みの分子生物学的解明に関する研究」平成14年度班会議  2003.2.13 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Effects of tyrosinase replacement in hemi-parkinsonian model

    Asanuma., M, Miyazaki, I, Higashi, Y, Tanaka, K, Ogawa, N

    32nd Annual Meeting of Society for Neuroscience  2002.11 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • L-DOPAおよびドパミンのキノン体生成を介したアポトーシス誘導性とそれに対する防御に関する検討

    小川紀雄, M. Emdadul Haque, 宮崎育子, 東 洋一郎, 浅沼幹人

    第32回日本神経精神薬理学会年会  2002.10.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メラニン合成酵素チロシナーゼのドパミン神経機能補完作用

    浅沼幹人, 宮崎育子, 東 洋一郎, 田中健一, 小川紀雄

    第32回日本神経精神薬理学会年会  2002.10.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メタフェタミンによるドパミン神経障害に対する非ステロイド性消炎鎮痛薬の効果

    辻 武史, 浅沼幹人, 宮崎育子, 田中健一, 小川紀雄

    第32回日本神経精神薬理学会年会  2002.10.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトにおけるドパミンレセプター発現と情報伝達系の存在に関する検討

    宮崎育子, 浅沼幹人, 東 洋一郎, 辻 武史, 小川紀雄

    第32回日本神経精神薬理学会年会  2002.10.18 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • パーキンソン病モデル線条体グリア細胞におけるドパミントランスポーターの発現誘導

    浅沼幹人, 宮崎育子, 東 洋一郎, 小川紀雄

    第45回日本神経化学会大会  2002.7.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンアゴニストcabergolineが示す神経保護効果の作用機序.

    吉岡真世, 田中健一, 藤田尚子, 宮崎育子, 浅沼幹人, 小川紀雄

    第45回日本神経化学会大会  2002.7.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトにおけるドパミントランスポーターを会したドパミン取り込み機構

    宮崎育子, 浅沼幹人, 東 洋一郎, 小川紀雄

    第45回日本神経化学会大会  2002.7.17 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • メラノサイトのパーキンソン病への移植に関する基礎検討. ワークショップ1「移植・再生医学の基礎と臨床」

    浅沼幹人, 宮崎育子, 東 洋一郎, 小川紀雄

    第101回岡山医学会総会  2002.6.1 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • チロシナーゼのParkinson病モデルに対する神経機能補完作用

    浅沼幹人, 宮崎育子, 東 洋一郎, 田中健一, 小川紀雄

    第43回日本神経学会総会  2002.5 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • L-DOPA連日投与によるパーキンソン病モデルの線条体グリア細胞におけるドパミントランスポーターの発現誘導

    小川紀雄, 浅沼幹人, 宮崎育子, 東 洋一郎, 田中健一

    厚生科学研究費補助金特定疾患対策研究事業「神経変性疾患に関する研究班」 平成13年度班会議  2002.1.12 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 6-OHDA誘導神経細胞死におけるPAG608の関与

    東 洋一郎, 浅沼幹人, 宮崎育子, M, Emdadul Haque, 小川紀雄

    第24回日本分子生物学会年会  2001.12.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Expression of tyrosinase, melanin synthetic enzyme, in hemi-parkinsonian model

    Asanuma, M, Higashi, Y. Miyazaki, I, Tanaka, K, Ogawa, N

    31st Annual Meeting of Society for Neuroscience  2001.11.15 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • メタンフェタミン神経毒性におけるp53関連遺伝子の関与

    浅沼幹人, 東 洋一郎, 宮崎育子, 辻 武史, 田中健一, 小川紀雄

    第31回日本神経精神薬理学会年会  2001.10.4 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンアゴニストropiniroleが示す神経保護作用の分子機序

    藤田尚子, 田中健一, 宮崎育子, 小川紀雄

    第44回日本神経化学会・第24回日本神経科学合同大会  2001.9.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 免疫抑制性ならびに非免疫抑制性イムノフィリンリガンドが示す細胞保護効果と作用機序

    田中健一, 藤田尚子, 宮崎育子, 吉岡真世, 東 洋一郎, 浅沼幹人, 小川紀雄

    第44回日本神経化学会・第24回日本神経科学合同大会  2001.9.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 6-hydroxydopamine誘導神経細胞死におけるPAG608の関与

    東 洋一郎, 浅沼幹人, 宮崎育子, M, Emdadul Haque, 小川紀雄

    第44回日本神経化学会・第24回日本神経科学合同大会  2001.9.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • アストロサイトにおけるドパミンレセプターおよびトランスポーターの発現

    宮崎育子, 浅沼幹人, 東 洋一郎, 小川紀雄

    第44回日本神経化学会・第24回日本神経科学合同大会  2001.9.27 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 非ステロイド性消炎鎮痛薬の一酸化窒素ラジカル消去作用と神経細胞死に対する保護効果

    浅沼幹人, 宮崎育子, 東洋一郎, 小川紀雄

    第12回中四国生体ラジカル研究会  2001.7.27 

     More details

    Language:Japanese  

    researchmap

  • Metallothionein-I, IIノックアウトマウスにおける6-hydroxydopamineによるドパミン神経毒性の増悪

    宮崎育子, 浅沼幹人, 東 洋一郎, 小川紀雄

    第28回日本脳科学会  2001.5.31 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Neuroprotective properties of GPI1046, a non-immunosuppressive immunophilin ligand

    Ogawa, N, Tanaka, K. Miyazaki, I, Fujita, N, Asanuma, M

    5th International Conference on Progress in Alzheimer's and Parkinson's Disease  2001.4.5 

     More details

    Language:English  

    researchmap

  • Direct redistribution and accumulation of dopaminergic drugs in the nucleus

    Asanuma, M, Miyazaki, I, Higashi, Y. Tanaka, K, Ogawa, N

    9th International Catecholamine Symposium  2001.4.4 

     More details

    Language:English  

    researchmap

  • Molecular mechanism in protective effects of cabergoline

    Tanaka, K, Miyazaki, I, Fujita, N, Asanuma, M, Ogawa, N

    9th International Catecholamine Symposium  2001.4.3 

     More details

    Language:English  

    researchmap

  • ニューロメラニン合成酵素チロシナーゼのドパミン神経障害への関与

    浅沼幹人, 東 洋一郎, 宮崎育子, 小川紀雄

    第9回カテコールアミン研究会  2001.4 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ニューロメラニン合成酵素チロシナーゼの機能異常によるドパミン神経障害

    小川紀雄, 浅沼幹人, 東洋一郎, 宮崎育子, 辻 武史

    厚生科学研究費補助金特定疾患対策研究事業「神経変性疾患に関する研究班」平成12年度班会議  2001.1.13 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Biochemical bases for neuroprotective effects of cabergoline

    Ogawa, N, Tanaka, K, Miyazaki, I, Fujita, N, Asanuma, M, Higashi, Y

    14th International Congress on Parkinson's Disease  2001 

     More details

    Language:English  

    researchmap

  • メタンフェタミンによるp53のDNA結合活性の変化と核内への直接移行・蓄積に関する検討

    浅沼幹人, 宮崎育子, 東 洋一郎, 田中健一, Cadet, J.L, 小川紀雄

    第30回日本神経精神薬理学会年会  2000.10.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 非免疫抑制性イムノフィリンリガンドGPI1046の抗酸化作用ならびに神経保護修復効果

    田中健一, 宮崎育子, 藤田尚子, 浅沼幹人, 吉岡真世

    第30回日本神経精神薬理学会年会  2000.10.26 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミンの核内への直接移行と集積に関する検討

    浅沼幹人, 宮崎育子, 田中健一, 小川紀雄

    第43回日本神経化学会  2000.10.20 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 非ステロイド性消炎鎮痛薬はNOによる神経細胞死を抑制する

    宮崎育子, 浅沼幹人, 小川紀雄

    第43回日本神経化学会  2000.10.19 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドパミン系神経細胞における増殖抑制因子メタロチオネイン-3の発現と動態

    東 洋一郎, 浅沼幹人, 宮崎育子, 小川紀雄

    第23回日本神経科学大会・第10回日本神経回路学会大会合同大会  2000.9.5 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Changes in expression on metallothionein-III messenger RNA in rat brain related to dopaminergic neurodegeneration and aging

    Ogawa, N, Miyazaki, K, Asanuma, M, Aoki-Sogawa, C

    XXIInd Congress of Collegium Internationale Neuro-Psychopharmacologium  2000.7.10 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Neuroprotective and anti-oxidative properties of the dopamine agonist cabergoline

    Tanaka, K, Miyazaki, I, Fujita, N, Ogawa, N

    22nd Collegium Internationale Neuro-Psychopharmacologicum (CINP)  2000.7.10 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ニューロンメラニン合成酵素チロシナーゼ機能抑制によるドパミン神経障害に関する検討

    浅沼幹人, 東 洋一郎, 宮崎育子, 小川紀雄

    第27回日本脳科学会  2000.5.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ドパミンアゴニストcabergolineの抗酸化作用と神経保護効果

    田中健一, 宮崎育子, 浅沼幹人, 藤田尚子, 小川紀雄

    第73回日本薬理学会年会  2000.3.23 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ドーパミン神経におけるチロシナーゼの機能解析

    東 洋一郎, 浅沼幹人, 宮崎育子, 小川紀雄

    第22回日本分子生物学会年会  1999.12.10 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • マウスグリア細胞におけるドーパミンにより誘導されるメタロチオネイン-III mRNAの発現制御機構

    十川千春, 十川紀夫, 宮崎育子, 浅沼幹人, 小川紀雄, 古田裕昭

    第2回メタロチオネイン研究会  1999.11.24 

     More details

    Language:Japanese  

    researchmap

  • ドパミン神経変性および加齢に伴う脳内metallochioneine-III mRNA発現の変化

    浅沼幹人, 宮崎育子, 東 洋一郎, 十川千春, 田中健一, 小川紀雄

    第2回メタロチオネイン研究会  1999.11.24 

     More details

    Language:Japanese  

    researchmap

  • ドパミンの核内転写因子への直接作用に関する検討

    浅沼幹人, 宮崎育子, 田中健一, 小川紀雄

    第42回日本神経化学会  1999.9.17 

     More details

    Language:Japanese  

    researchmap

  • 加齢による脳内metallothionein-III mRNA発現の変化

    宮崎育子, 浅沼幹人, 十川千春, 田中健一, 東 洋一郎, 小川紀雄

    第42回日本神経化学会  1999.9.16 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • D2レセプターを介するドパミンアゴニストropiniroleの抗酸化作用ならびに神経保護効果

    小川紀雄, 田中健一, 宮崎育子, 浅沼幹人, 飯田基之

    第29回日本神経精神薬理学会年会  1999.9.14 

     More details

    Language:Japanese  

    researchmap

  • 6-OHDA投与後の脳内酸化ストレス消去系の経時変化

    田中健一, 宮崎育子, 藤田尚子, 浅沼幹人, 小川紀雄

    第29回日本神経精神薬理学会年会  1999.9.14 

     More details

    Language:Japanese  

    researchmap

  • Dopamine D2 receptor mediated antioxidant and neuroprotective effects of ropinirole.

    Ogawa, N, Miyazaki, I, Tanaka, K, Iida, M, Asanuma, M

    XIII International Congress on Parkinson's Disease  1999.7.27 

     More details

    Language:English  

    researchmap

  • ドパミンアゴニストropiniroleの神経保護作用とその機序について

    飯田基之, 宮崎育子, 田中健一, 浅沼幹人, 小川紀雄

    第26回日本脳科学会  1999.5.14 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • マウスグリア細胞株(VR-2g)におけるドーパミンにより誘導されるメタロチオネイン-III (GIF) mRNAの発現制御機構

    十川千春, 十川紀夫, 宮崎育子, 浅沼幹人, 小川紀雄, 古田裕昭

    第72回日本薬理学会年会  1999.3.24 

     More details

    Language:Japanese  

    researchmap

▼display all

Industrial property rights

  • キノン体若しくはキノン体前駆体の毒性に起因する障害に対する予防治療剤

     More details

    Application no:特願2005-27094 

    特許第4734560号

    researchmap

Awards

  • 平成18年度岡山医学会賞 新見賞

    2007  

     More details

    Country:Japan

    researchmap

  • 第32回日本脳科学会 学会奨励賞

    2005  

     More details

    Country:Japan

    researchmap

  • 第4回メタロチオネイン研究会 研究奨励賞

    2003  

     More details

    Country:Japan

    researchmap

Research Projects

  • アストロサイトの部位特異性に基づくα-シヌクレイン動態および神経障害機構の解明

    Grant number:19K07993  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    宮崎 育子, 浅沼 幹人

      More details

    Authorship:Principal investigator 

    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    アストロサイトが神経細胞内α-シヌクレイン(α-syn)動態に及ぼす影響と環境毒ロテノン誘発神経障害との連関を解析した.中脳あるいは線条体アストロサイト培養液添加による中脳神経細胞内α-syn発現変化を検討するために,15日齢SDラット胎仔からの中脳神経細胞に中脳アストロサイト培養液あるいは線条体アストロサイト培養液を添加し,チロシン水酸化酵素(TH),グルタミン酸脱炭酸酵素(GAD),トリプトファン水酸化酵素(TPH2)とα-synの二重染色を行った.中脳アストロサイト培養液の添加により,中脳神経細胞におけるα-syn発現が誘導され,これは主にTPH2陽性セロトニン神経細胞とTH陽性ドパミン神経細胞で惹起されることがわかった.一方,線条体アストロサイト培養液添加では中脳神経細胞におけるα-syn発現変化はみられなかった.ロテノン(5 nM)を48時間曝露した中脳あるいは線条体アストロサイトの培養液で中脳神経細胞を処置すると,TPH2陽性セロトニン神経細胞ではα-syn発現がさらに誘導され,神経細胞数は増加した.また,GAD陽性グルタミン酸神経細胞では,ロテノン処置した中脳アストロサイト培養液添加でα-syn発現および細胞生存率ともに変化はみられなかった.一方,TH陽性ドパミン神経細胞では,ロテノン曝露中脳アストロサイト培養液処置により細胞生存率が約70%に低下し,残存する神経細胞におけるα-syn発現は一様ではなく細胞により異なる動態を示した.これはドパミン神経細胞が障害される過程でα-syn発現動態が変化することを示唆する.
    以上の結果より,中脳アストロサイトが分泌する何らかの因子がセロトニン神経細胞やドパミン神経細胞におけるα-syn発現を高発現させること,細胞内α-syn発現に加え他の要因がロテノン誘発ドパミン神経障害に関与することが示唆された.

    researchmap

  • Studies of rotenone-induced dopaminergic neurodegeneration focusing on brain region-specific astrocyte dysfunction.

    Grant number:16K09673  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Miyazaki Ikuko

      More details

    Authorship:Principal investigator 

    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    In this study, we revealed that astrocyte-conditioned media from low-dose rotenone-treated mesencephalic, but not striatal, astrocytes produced dopaminergic neuron-specific neurotoxicity, and that endogenous dopamine and its oxidation play an important role in mesencephalic astrocyte-mediated dopaminergic neurodegeneration. In addition, we demonstrated that reduction of antioxidative molecule metallothionein (MT) in enteric glial cells after rotenone-exposure induced enteric neurotoxicity. Oral administration of coffee components, caffeic acid and chlorogenic acid, upregulated MT expression both in the striatal astrocytes and enteric glial cells, and inhibited degeneration of central dopaminergic and peripheral enteric neurons in rotenone-treated mice. These results suggest possible involvement of brain region-specific glial dysfunction in neurotoxicity induced by environmental toxin rotenone.

    researchmap

  • アストロサイトにおけるNFAT関連分子の部位特異的誘導がもたらす神経変性

    2014

    岡山医学振興会研究助成 

    宮崎育子

      More details

    Authorship:Principal investigator 

    researchmap

  • アストロサイトにおけるオートファジーに着目した環境毒誘発パーキンソニズムの病態解析

    2014

    山陽放送学術文化財団研究助成 

    宮崎育子

      More details

    Authorship:Principal investigator 

    researchmap

  • Studies on mechanism of rotenone-induced neurodegeneration in central and peripheral nervous systems and involvement of neuron-astrocyte interaction.

    Grant number:25461279  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MIYAZAKI IKUKO, ASANUMA MASATO

      More details

    Authorship:Principal investigator 

    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    In this study, we revealed that subcutaneous administration of rotenone caused progressive neurodegeneration in the olfactory bulb and myenteric plexus of ascending colon, in addition to the nigrostriatal pathway, but not in the thoracic cord. Rotenone treatment also induced increases in the number of astrocytes in a time- and region-specific manner. These results suggested that neurodegeneration after rotenone exposure was region-specific, but did not spread retrogradely from the peripheral tissue to nigrostriatal pathway, and that glial activation could be related to rotenone-induced neurotoxicity. Using primary cultured cells, we showed that rotenone induced activation of NFAT, secretion of inflammatory cytokines, and decreased metallothionein release in/from rotenone-treated mesencephalic astrocytes, which caused dopaminergic neurotoxicity. These results suggest possible involvement of glial dysfunction in dopaminergic neurotoxicity induced by environmental toxin rotenone.

    researchmap

  • Systemic neurodegeneration and involvement of astroglial cells in Parkinson's disease.

    Grant number:22590934  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MIYAZAKI Ikuko, ASANUMA Masato, NAKAMURA Kazufumi

      More details

    Authorship:Principal investigator 

    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    In this study, we revealed that GFAP-positive glial cells were distributed along the myenteric plexus in the ileum and ascending colon of rotenone-chronically treated rats, preceding central dopaminergic neurodegeneration, suggesting possible involvement of enteric glial cells in rotenone-induced cytotoxicity of enteric neuronal cells which precedes neurodegeneration in central nervous system. Using primary cultured cells, we also proposed a possible involvement of some molecules secreted from rotenone-treated mesencephalic astroglia in rotenone-induced dopaminergic neurotoxicity, and revealed that metallothionein could protect dopaminergic neurons against rotenone toxicity.

    researchmap

  • ドパミン神経選択的障害因子ドパミンキノンによる神経障害に対するアストログリアの抗酸化機構の役割に関する研究

    2008

    (財)川崎医学・医療福祉学振興会教育研究助成 

    宮崎育子

      More details

    Authorship:Principal investigator 

    researchmap

  • ドパミンキノン神経障害を含む脳内酸化ストレスに対するアストログリアの抗酸化機構の解析

    2008

    岡山医学振興会研究助成 

    宮崎育子

      More details

    Authorship:Principal investigator 

    researchmap

  • シナプス小胞外の遊離ドパミンによるドパミン神経障害とその保護に関わる分子の探索

    Grant number:18700364  2006 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    宮崎 育子

      More details

    Authorship:Principal investigator 

    Grant amount:\3500000 ( Direct expense: \3500000 )

    1.内在性キノン消去系分子の検索
    小胞外ドパミン(DA)過剰モデル細胞においてキノン還元酵素NQO1が増加しており,DA神経毒6-OHDAによる片側パーキンソン病(PD)モデルマウスの線条体では,NQO1が増加し,NQOI遺伝子の転写因子Nrf2の神経細胞での活性化ならびにDAキノン(DAQ)消去にはたらくグルタチオン(GSH)の合成基質のアストログリアでの取り込み部位の発現増加がみられることを組織学的に確認できた.
    2.内在性キノン消去系分子のキノン毒性に対する保護効果
    DA系神経細胞CATH.aへの過剰DA添加あるいはメタンフェタミン(METH)添加による小胞外過剰DA状態でのキノン結合蛋白キノプロテイン(QP:DAQ生成の指標)の増加と細胞障害性が,NQO1の賦活薬BHAの前処置により抑制できることを明らかにした.また,昨年度の検索でDAQを消去することがわかった内在性キノン消去分子の金属結合蛋白メタロチオネイン1(MT1)のキノン毒性への効果について検討した.CATH.a細胞への過剰DA添加によるQP増加ならびに細胞死は,MT1を誘導す亜鉛添加により抑制できた.
    3.内土性キノン消去系分子ノックダンによるドパミン神経障害増悪の検討
    昨年度,DAQを酸化させるチロシナーゼの欠損マヴスの線条体では,METH投与によるDATの脱落が野生型に比べて著明に増悪することを報告したが,CATH.a細胞へのチロシナーゼ阻害薬添加によりMETH神経毒性が増悪することも確認できた.また,DAQを無毒化することが明らかになった中枢神経作用薬の投与により,大脳基底核のGSH量が増加し,PDモデル障害側黒質でのDA神経の脱落および障害側線条体でのL-DOPA連日投与によるQPの著増が抑制された.さらに,片側PDモデルの障害側のDA神経障害は,野生型に比べMT1ノックアウトマウスでより顕著であった.
    DAQ消去にはたらくGSH,NQO1,Nrf2,チロシナーゼ,MT1などのシステイン基含有分子およびこれらの発現を高める薬剤が,小胞外遊離DAによるDA神経毒性に対して保護的にはたらくことを明らかにできた.

    researchmap

  • パミン神経特異的酸化ストレスとしてのドパミンキノンを指標にした非ステロイド性消炎鎮痛薬のドパミン神経保護効果のプロファイリング

    2006

    カテコールアミンと神経疾患研究会研究助成 

    宮崎育子

      More details

    Authorship:Principal investigator 

    researchmap

  • パーキンソン病のL-DOPA投与による副作用発現とグリアのドパミン取込みとの連関

    Grant number:15790450  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    宮崎 育子

      More details

    Authorship:Principal investigator 

    Grant amount:\3200000 ( Direct expense: \3200000 )

    昨年度,ラット胎児中脳あるいは線条体からの初代培養アストロサイトにおいてドパミンD1,D3,D4,D5レセプターおよびドパミントランスポーター(DAT)が発現しており,L-DOPA投与によりパーキンソン病(PD)モデル傷害側のアストロサイトでのドパミンの取り込みおよびDATの発現増加が認められることを明らかにした.本年度は,初代培養アストロサイトにおけるドパミンの取り込み,L-DOPAからのドパミン産生能,PDモデル脳のミクログリアにおけるドパミン取り込みについて検討した.
    1.ドパミン神経障害時の培養アストロサイトでのドパミンの取込み動態の変化
    線条体からのニューロン・アストロサイト混合初代培養系に6-OHDAあるいはLPSで前処置を行い,さらにドパミンを添加しドパミン神経障害を惹起させると,アストロサイトへのドパミンの取り込みがみられ,DATの発現が増加することを蛍光二重染色により明らかにした.さらに,このときアストロサイト細胞内においてドパミンが増加することを高速液体クロマトグラフィーにより検出した.
    2.培養アストロサイトにおけるL-DOPAからのドパミン産生機構
    線条体からの初代培養アストロサイトにおいて,DOPA脱炭酸酵素およびL型中性アミノ酸トランスポーターが発現していることをWestern blot法により確認した.このことから,L-DOPAはアストロサイトに取り込まれ,ドパミンに変換されている可能性が考えられる.
    3.パーキンソン病モデル脳のミクログリアにおけるドパミン取り込み
    6-OHDA注入による片側PDモデルラットにL-DOPAを1週間連日投与し,最終投与3時間後の脳凍結切片を用いてドパミンとOX-42(ミクログリアマーカー)の蛍光二重染色を行った.PDモデル傷害側線条体でミクログリアの著しい増殖がみられたが,ミクログリアへのドパミンの取り込みは認められなかった.

    researchmap

  • グリア機能不全に着目した環境毒誘発パーキンソン病における腸管神経変性機構の解明

    Grant number:21K07415  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    浅沼 幹人, 宮崎 育子

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    researchmap

  • Basic construction for medical treatment of oral cancer by controlling of metal-binding protein expression utilizing optogenetics

    Grant number:20K10151  2020.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    十川 紀夫, 今村 泰弘, 十川 千春, 宮崎 育子, 村上 聡, 荒 敏昭

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    researchmap

  • 日本語話者における発達性読み書き障害の病因遺伝子解析

    Grant number:19K07802  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    岡 牧郎, 宮崎 育子

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\2470000 ( Direct expense: \1900000 、 Indirect expense:\570000 )

    本研究は、日本語話者の発達性読み書き障害(developmental dyslexia:DD)患者において、病因となる遺伝子変異の有無を明らかにすることを目的としている。また、日本語話者のDD患者に海外での報告にない特異的な遺伝子変異の有無を確認したい。さらには、遺伝子変異の有無によるDDの臨床像の違いを検討する。この研究は日本で初めての取り組みであり、研究成果はDDの病態生理に関する研究の進歩やDDの治療教育に貢献できると考える。
    2019年度は共同研究者と討議を重ね、口腔内検体からの安定したDNA抽出方法や遺伝子解析方法など検討した。欧米諸国で報告されている候補遺伝子(DYX1C1, DCDC2, KIAA0319, ROBO1など)において、それぞれで報告のある一塩基多型(SNP)変異と同様の変異がみられるかどうかを対照群とともにリアルタイムPCRで検討し、その後に特異的な遺伝子変異の有無についても検討する方針であった。現在は、これらのSNP変異をまとめて検討することが可能なマイクロアレイを行う方針にした。準備が整ったため、現在は検体採取段階である。

    researchmap

  • Foundation of medical treatment for tongue cancer by the regulation of metal-binding protein

    Grant number:17K11689  2017.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Sogawa Norio

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    In order to construct a foundation of medical treatment for tongue cancer, it was investigated the influence of the regulation of MT-4 expression on cancer cell growth, an intracellular zinc ion regulating protein expressed specifically in squamous epithelium. Moreover, it was explored the inducers of MT-4 gene expression and intracellular interacted factor with MT-4 protein.
    As the results, it was revealed that the zinc sensitivity was associated with cell proliferation of cancer, and zinc sensitive cancer cells decreased the cell proliferation by MT-4 gene introduction. Moreover, it was suggested that MT-4 gene expression was induced by glucocorticoids and MT-4 was related with gene expression by itself.

    researchmap

  • Development of neuroprotective therapy for amyotrophic lateral sclerosis using serotonergic reagent

    Grant number:24659431  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    ASANUMA Masato, MIYAZAKI Ikuko

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\3770000 ( Direct expense: \2900000 、 Indirect expense:\870000 )

    We examined neuroprotective effects of serotonin-1A receptor agonist 8-OH-DPAT on progressive motor neuron degeneration in amyotrophic lateral sclerosis (ALS) model mice and in primary culture cells. The pre-treatments of 8-OH-DPAT prior to onset of motor symptoms delayed disease onset. The chronic post-treatments of the drug after the onset significantly inhibited disease progression and showed protective effects on motor neuron loss in the cervical and lumber spinal cords in the ALS models. Furthermore, we revealed that the neuroprotective effects are based, in part, on expression and release of metallothionein in/from astrocytes via serotonin-1A receptors and sequent activation of transcription factor Nrf2.

    researchmap

  • A novel therapeutic strategy for Parkinson's disease by activation of dopamine quinone-sensing molecules in astroglia

    Grant number:21591082  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    ASANUMA Masato, MIYAZAKI Ikuko

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    We revealed that induction and sequent release of glutathione or metallothionein in astroglia play an antioxidative protective role against neurotoxicity induced by dopamine neuron-specific oxidative stress in this study. Using neuron-astroglia coculture and parkinsonian model mice, we also proposed a possible therapeutic strategy for Parkinson's disease that a serotonin receptor agonist can protect dopamine neurons via activation of dopamine quinone-sensing molecules in astroglia.

    researchmap

  • Studies on dopamine neuron-specific dmenerative factor dopamine quinone in the pathogenesis of Parkinson's disease.

    Grant number:17590878  2005 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    ASANUMA Masato, MIYAZAKI Ikuko

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\3700000 ( Direct expense: \3400000 、 Indirect expense:\300000 )

    To clarify the possible roles of dopamine neuron-specific degenerative factor dopamine quinone in the pathogenesis of Parkinson's disease and to investigate therapeutic application of the dopamine quinone-regulating agents, we performed following experiments using dopaminergic neuronal cultured cells and hemi-parkinsonian model mice.
    1. Screening of interacting molecules with dopamine or dopamine quinone
    By electron spin resonance method using in vitro dopamine quinone generating system, we identified metallothionein-1, some dopamine agonists and certain drug as dopamine quinone-interacting molecules.
    2. Dopamine quinone-induced toxicity in cultured neuronal cells and effects of regulation of quinone-quenching system
    In dopaminergic CATH.a cells, dopamine quinone generated from excess free dopamine exerts neurotoxic effects. However, the dopamine-quinone-induced neurotoxicity was prevented by quenching of dopamine quinone, e.g. quinone reductase inducer, metallothionein inducer and a drug which enhances up-take of the substrate of glutathione synthesis in astroglial cells.
    3. Changes in quinone-quenching system and effects of its regulation in parkinsonian animal models
    Several molecules of quinone-quenching system were activated in the lesioned striatum of hemi-parkinsonian mice. The drug, which interacts with dopamine quinone, enhanced astroglial proliferation, up-take of glutathione substrate in astroglial cells, and increased glutathione levels in the striatum. Furthermore, the drug ameliorated dopaminergic neuronal loss in the substantia nigra and inhibited repeated L-DOPA injections-induced elevation of protein-bound quinone (quinoprotein) in the striatum. Repeated L-DOPA administration markedly elevated striatal quinoprotein levels and reduced the dopaminergic nerve terminals specifically on the lesioned side in metallothionein-knockout parkinsonian mice, suggesting that intrinsic cysteine-rich molecule metallothionein protects against L-DOPA-induced dopamine quinone neurotoxicity in parkinsonian mice by its quinone-quenching property.
    These experimental results suggest a therapeutic potency of dopamine quinone-quenching agents for the patients of Parkinson's disease, and clarify that neuro-glial interaction on up-take of the substrate of glutathione synthesis plays a role in neuroprotective strategies against progressive dopaminergic neurodegeneration.

    researchmap

  • Studies on tyrosinase-producing cell transplantation into parkinsonian models.

    Grant number:14570596  2002 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    ASANUMA Masato, MIYAZAKI Ikuko

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\3200000 ( Direct expense: \3200000 )

    To investigate the possible effects of tyrosinase-producing cell transplantation in parkinsonian models, we performed intrastriatal transplantation of melanocytes or melanoma cells, as tyrosinase-producing cells, into hemi-parkinsonian model animals, as follows :
    1.Transplantation of melanoma cells into the striatum of parkinsonian models
    Intrastriatal transplantation of B16-F1 melanoma cells, as tyrosinase-producing cells, into hemi-parkinsonian rats lesioned by 6-OHDA markedly ameliorated apomorphine-induced rotation behavior towards contralateral side up to 40 days after the transplantation of melanoma cells, coinciding with expression of dopamine-positive signals around survived transplant.
    2.Primary cultured melanocvtes from the back of newborn mice
    We established the procedure of primary culture for melanocytes from the back of newborn C57BL mice, which showed higher tyrosinase activity than melanocytes from albino mice.
    3.Transplantation of primary_cultured melanocytes into the striatum of parkinsonian models
    The transplantation of primary cultured melanocytes from the back of newborn black mice into the lesioned striatum of hemi-parkinsonian mice markedly and continuously ameliorated the apomorphine-induced contralateral rotation behavior up to 3 months after the melanocyte transplantation. In the transplanted group with melanocytes, the striatal transplant melanocytes survived coinciding with dopamine- and tyrosinase-positive signals along the transplant in the striatum 3 months after the transplantation.
    4.Primary cultured melanocytes from the adult mice
    We tried to establish the culture of melanocytes from the back skin of adult C57BL mice for future auto-transplantation of melanocytes into parkinsonian models, but failed to do it because of contamination of the folliculi.
    5.Distribution of tyrosinase-positive cells in the brain of parkinsonian models
    Tyrosinase constitutively expressed mainly on oligodendrocytes in the corpus callosum, striatum and substantia nigra. In the parkinsonian model mice, tyrosinase-positive oligodendrocytes increased in the striatum, whereas it decreased in the substantia nigra.
    These experimental results suggest a therapeutic potency of tyrosinase-producing cells such as melanocytes which may complement tyrosine hydroxylase when dopaminergic neurons are degenerated in Parkinson's disease, and may normalize L-DOPA-induced abnormal dopamine turnover

    researchmap

▼display all

 

Class subject in charge

  • Primary Anatomy (2021academic year) special  - その他

  • Human Anatomy (2021academic year) Concentration  - その他

  • Neuroanatomy (2021academic year) special  - その他

  • Practice in Neuroanatomy (2021academic year) special  - その他

  • Lecture: Cerebral stroke (2021academic year) special  - その他

  • Research Projects and Practicals: Medical Neurobiology I (2021academic year) special  - その他

  • Lecture and Research Projects: Medical Neurobiology I (2021academic year) special  - その他

  • Research Projects and Practicals: Medical Neurobiology II (2021academic year) special  - その他

  • Lecture and Research Projects: Medical Neurobiology II (2021academic year) special  - その他

  • Primary Anatomy (2020academic year) special  - その他

  • Neuroanatomy (2020academic year) special  - その他

  • Practice in Neuroanatomy (2020academic year) special  - その他

  • Research Projects and Practicals: Medical Neurobiology I (2020academic year) special  - その他

  • Lecture and Research Projects: Medical Neurobiology I (2020academic year) special  - その他

  • Research Projects and Practicals: Medical Neurobiology II (2020academic year) special  - その他

  • Lecture and Research Projects: Medical Neurobiology II (2020academic year) special  - その他

▼display all