2021/12/22 更新

写真a

オオノ アユム
大野 歩
Ohno Ayumu
所属
医歯薬学域 助教(特任)
職名
助教(特任)
外部リンク

学位

  • 博士(医学) ( 2013年3月   東北大学 )

研究分野

  • ライフサイエンス / 分子生物学

  • ライフサイエンス / 細菌学

  • ライフサイエンス / ウイルス学

学歴

  • 東北大学大学院 医学系研究科 博士課程    

    2009年4月 - 2013年3月

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  • 金沢大学大学院 医学系研究科 修士課程    

    2007年4月 - 2009年3月

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  • 東海大学 開発工学部 生物工学科    

    2003年4月 - 2007年3月

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経歴

  • Collaborative Research Center of OKAYAMA University for Infectious Diseases in India at NICED

    2021年11月 - 現在

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  • 岡山大学   大学院医歯薬学総合研究科   助教(特任)

    2021年9月 - 現在

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  • 東海大学   医学部基礎医学系分子生命科学情報生物医学研究室   研究員

    2017年9月 - 2021年8月

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  • 富山大学   大学院医学薬学研究部(医学)ウイルス学講座   助教

    2016年4月 - 2017年7月

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  • 富山大学   大学院医学薬学研究部(医学)ウイルス学講座   研究員

    2015年8月 - 2016年3月

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  • 理化学研究所   分子ウイルス学特別研究ユニット   特別研究員

    2013年6月 - 2015年7月

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▼全件表示

所属学協会

 

論文

  • Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer 国際誌

    Ayumu Ohno, Kazuo Umezawa, Satomi Asai, Kirill Kryukov, So Nakagawa, Hayato Miyachi, Tadashi Imanishi

    Scientific Reports   11 ( 1 )   3436 - 3436   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Spread of drug-resistant bacteria is a serious problem worldwide. We thus designed a new sequence-based protocol that can quickly identify bacterial compositions of clinical samples and their drug-resistance profiles simultaneously. Here we utilized propidium monoazide (PMA) that prohibits DNA amplifications from dead bacteria, and subjected the original and antibiotics-treated samples to 16S rRNA metagenome sequencing. We tested our protocol on bacterial mixtures, and observed that sequencing reads derived from drug-resistant bacteria were significantly increased compared with those from drug-sensitive bacteria when samples were treated by antibiotics. Our protocol is scalable and will be useful for quickly profiling drug-resistant bacteria.

    DOI: 10.1038/s41598-021-82903-z

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  • Cholera Rapid Diagnostic Tests for the Detection of Vibrio cholerae O1: An Updated Meta-Analysis. 国際誌

    Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Anusuya Debnath, Keinosuke Okamoto, Shin-Ichi Miyoshi

    Diagnostics (Basel, Switzerland)   11 ( 11 )   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The rapid diagnosis of cholera contributes to adequate outbreak management. This meta-analysis assesses the diagnostic accuracy of cholera rapid tests (RDTs) to detect Vibrio cholerae O1. METHODS: Systematic review and meta-analysis. We searched four databases (Medline, EMBASE, Google Scholar, and Web of Science up to 8 September 2021) for studies that evaluated cholera RDTs for the detection of V. cholerae O1 compared with either stool culture or polymerase chain reaction (PCR). We assessed the studies' quality using the QUADAS-2 criteria. In addition, in this update, GRADE approach was used to rate the overall certainty of the evidence. We performed a bivariate random-effects meta-analysis to calculate the pooled sensitivity and specificity of cholera RDTs. RESULTS: Overall, 20 studies were included in this meta-analysis. Studies were from Africa (n = 11), Asia (n = 7), and America (Haiti; n = 2). They evaluated eight RDTs (Crystal VC-O1, Crystal VC, Cholkit, Institut Pasteur cholera dipstick, SD Bioline, Artron, Cholera Smart O1, and Smart II Cholera O1). Using direct specimen testing, sensitivity and specificity of RDTs were 90% (95% CI, 86 to 93) and 86% (95% CI, 81 to 90), respectively. Cholera Sensitivity was higher in studies conducted in Africa [92% (95% CI, 89 to 94)] compared with Asia [82% (95% CI, 77 to 87)]. However, specificity [83% (95% CI, 71 to 91)] was lower in Africa compared with Asia [90% (95% CI, 84 to 94)]. GRADE quality of evidence was estimated as moderate. CONCLUSIONS: Against culture or PCR, current cholera RDTs have moderate sensitivity and specificity for detecting Vibrio cholerae O1.

    DOI: 10.3390/diagnostics11112095

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  • Examination of the microbiota of normal cow milk using MinIONTM nanopore sequencing.

    Yasunori Shinozuka, Kazuhiro Kawai, Tomomi Kurumisawa, Yuko Shimizu, Tadashi Imanishi, Ayumu Ohno, Mano Takahashi, Sohei Kaneko, Naoki Suzuki

    The Journal of veterinary medical science   83 ( 11 )   1620 - 1627   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this study was to evaluate the microbiota of normal milk in dairy cows and their relationship with host factors, such as the age of the cow (Age), somatic cell counts in milk (SCCs), and days in milk (DIM). We investigated 48 milk samples from 22 cows with no systemic or local clinical signs using MinIONTM nanopore sequencing for a 16S rRNA gene amplicon. Bacterial richness was positively correlated with the DIM (P=0.043), and both the Shannon-Wiener Index and Simpson's Index, which are metrics of alpha-diversity, were also significantly positively correlated with the SCC (P<0.001). The composition ratios of both Actinobacteria at the phylum level and Kocuria spp. at the genus level in the milk microbiota were significantly correlated with the SCC (P<0.001 and P<0.001, respectively). In the beta-diversity test, the one-way analysis of similarities test showed a significant difference (P=0.0051) between the low- and high-SCC groups. This study clarified that the composition of the normal milk microbiota in this herd was related to the SCC. It also raised the possibility of variations in bacterial genera in the normal milk microbiota between the low- and high-SCC groups. However, to clarify the actual condition of the milk microbiota and to elucidate the relationship with the SCC, it is necessary to perform further analyses taking into account not only the relative abundance, but also the absolute abundance of microbes.

    DOI: 10.1292/jvms.21-0353

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  • Human short tandem repeat identification using a nanopore-based DNA sequencer: a pilot study 査読 国際誌

    Minoru Asogawa, Ayumu Ohno, So Nakagawa, Eriko Ochiai, Yasuhiro Katahira, Megumi Sudo, Motoki Osawa, Masatoshi Sugisawa, Tadashi Imanishi

    Journal of Human Genetics   65 ( 1 )   21 - 24   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Short tandem repeats (STRs) are repetitive DNA sequences that are highly polymorphic and widely used for personal identification in the field of forensic medicine. The standard method for determining the repeat number of STRs is capillary electrophoresis of PCR products; however, the use of DNA sequencing has increased because it can identify same-sized alleles with nucleotide substitutions (iso-alleles). In this study, we performed human STR genotyping using a portable nanopore-based DNA sequencer, the MinION, and evaluated its performance. Because the sequence quality obtained by MinION is considerably lower than those obtained with other DNA sequencers, we developed an original scoring scheme for judging the genotypes from MinION reads. Analysis of seven human samples for 21–45 STR loci yielded an average of 857 thousand reads per sample, and the accuracy of genotyping and iso-allele identification reached 75.7% and 82%, respectively. Although the accuracy is higher than that reported previously, further improvements are required before this method can be practically applied.

    DOI: 10.1038/s10038-019-0688-z

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  • Bovine leukemia virus proviral load is more strongly associated with bovine major histocompatibility complex class II DRB3 polymorphism than with DQA1 polymorphism in Holstein cow in Japan 査読 国際誌

    Shin Nosuke Takeshima, Ayumu Ohno, Yoko Aida

    Retrovirology   16 ( 1 )   14 - 14   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bovine leukemia virus (BLV) causes enzootic bovine leukosis and is closely related to the human T-lymphotropic virus. Bovine major histocompatibility complex (BoLAs) are used extensively as markers of disease and immunological traits in cattle. For BLV diagnosis, proviral load is a major diagnosis index for the determination of disease progression and transmission risk. Therefore, we investigated the frequency of BoLA-DRB3 alleles, BoLA-DQA1 alleles, and haplotypes of BoLA class II isolated from the heads of 910 BLV-infected cows out of 1290 cows assessed from BLV-positive farms, in a nationwide survey from 2011 to 2014 in Japan. Our aim was to identify BoLA class II polymorphisms associated with the BLV proviral load in the Holstein cow. The study examined 569 cows with a high proviral load and 341 cows with a low proviral load. Using the highest odds ratio (OR) as a comparison index, we confirmed that BoLA-DRB3 was the best marker for determining which cow spread the BLV (OR 13.9 for BoLA-DRB3, OR 11.5 for BoLA-DQA1, and OR 6.2 for BoLA class II haplotype). In addition, DRB3002:01,009:02,012:01,014:01, and015:01 were determined as BLV provirus associated alleles. BoLA-DRB3002:01,009:02, and014:01 were determined as resistant alleles (OR > 1), and BoLA-DRB3012:01 and015:01 were determined as susceptible alleles (OR < 1). In this study, we showed that BoLA-DRB3 was a good marker for determining which cow spread BLV, and we found not only one resistant allele (BoLA-DRB3009:02), but also two other disease-resistant alleles and two disease-susceptible alleles. This designation of major alleles as markers of susceptibility or resistance can allow the determination of the susceptibility or resistance of most cows to disease. Overall, the results of this study may be useful in eliminating BLV from farms without having to separate cows into several cowsheds.

    DOI: 10.1186/s12977-019-0476-z

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  • Rapid sequencing-based diagnosis of infectious bacterial species from meningitis patients in Zambia 査読 国際誌

    So Nakagawa, Shigeaki Inoue, Kirill Kryukov, Junya Yamagishi, Ayumu Ohno, Kyoko Hayashida, Ruth Nakazwe, Mox Kalumbi, Darlington Mwenya, Nana Asami, Chihiro Sugimoto, Mable M. Mutengo, Tadashi Imanishi

    Clinical and Translational Immunology   8 ( 11 )   e01087   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Objectives: We have developed a portable system for the rapid determination of bacterial composition for the diagnosis of infectious diseases. Our system comprises of a nanopore technology-based sequencer, MinION, and two laptop computers. To examine the accuracy and time efficiency of our system, we provided a proof-of-concept for the detection of the causative bacteria of 11 meningitis patients in Zambia. Methods: We extracted DNA from cerebrospinal fluid samples of each patient and amplified the 16S rRNA gene regions. The sequencing library was prepared, and the sequenced reads were simultaneously processed for bacterial composition determination using the minimap2 software and the representative prokaryote genomes. Results: The sequencing results of four of the six culture-positive samples were consistent with those of conventional culture-based methods. The dominant bacterial species in each of these samples were identified from the sequencing data within only 3 min. Although the major bacterial species were also detected from the other two culture-positive samples and five culture-negative samples, their presence could not be confirmed. Moreover, as a whole, although the number of sequencing reads obtained within a short sequencing run was small, there was no change in the major bacterial species over time with prolonged sequencing. In addition, the processing time strongly correlated with the number of sequencing reads used for the analysis. Conclusion: Our results suggest that time-effective analysis could be achieved by determining the number of sequencing reads required for the rapid diagnosis of infectious bacterial species depending on the complexity of bacterial species in a sample.

    DOI: 10.1002/cti2.1087

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  • Improved photocatalytic air cleaner with decomposition of aldehyde and aerosol-associated influenza virus infectivity in indoor air 査読

    Kimiyasu Shiraki, Hiroshi Yamada, Yoshihiro Yoshida, Ayumu Ohno, Teruo Watanabe, Takafumi Watanabe, Hiroyuki Watanabe, Hidemitsu Watanabe, Masao Yamaguchi, Fumio Tokuoka, Shigeatsu Hashimoto, Masakazu Kawamura, Norihisa Adachi

    Aerosol and Air Quality Research   17 ( 11 )   2901 - 2912   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AAGR Aerosol and Air Quality Research  

    Air pollution caused by fine particulate matter (PM2.5), volatile organic compounds, and bioaerosols is a major environmental risk to health. We developed a photocatalytic air cleaner for reducing the pollution levels of indoor air; we improved the photocatalytic system by using UV-LED for the removal of acetaldehyde and PM2.5 and by reducing the weight and size of the system. The efficiency of photocatalysis depends on the surface area and materials. Therefore, we prepared a nanosized titanium dioxide (TiO2)-coated aluminum plate irradiated by UV-LED lamps (wavelength: 375 nm) as a photocatalytic air cleaner. Passing air continuously through a TiO2-coated aluminum plate (5 × 10 × 1 cm) under black light for 200 min decomposed 90% of 5 ppm acetaldehyde (12.4 µmol h–1) and generated two carbon dioxide molecules (25.43 µmol h–1) at a molar ratio of 1:2, indicating complete decomposition of acetaldehyde with high efficiency. This photocatalytic air cleaner was applied to the decomposition of acetaldehyde and inactivation and removal of aerosol-associated influenza virus. Acetaldehyde (20 ppm) in a 1-m3 cubic space was eliminated in 60 min at a half-life of 8 min. The aerosol-associated infectivity and the RNA genome of influenza virus A/PR/8/1934 (H1N1) produced by a nebulizer in a 779-L cubic space were eliminated within 7 min; however, they were detectable for up to 28 minutes when the functional photocatalytic air cleaner was not used. The presence of intermediate breakdown products of influenza virus indicated that the virus was broken down by photocatalysis. Thus, the photocatalytic air cleaner efficiently decomposed and eliminated organic chemicals, acetaldehyde, and aerosol-associated influenza virus infectivity and viral RNA, indicating that it can clean and detoxify the indoor air in a closed space for maintaining a safer environment.

    DOI: 10.4209/aaqr.2017.06.0220

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  • Interaction of Immunoglobulin with Cytomegalovirus-Infected Cells. 査読 国際誌

    Nobuyasu Aiba, Atsuko Shiraki, Misako Yajima, Yukari Oyama, Yoshihiro Yoshida, Ayumu Ohno, Hiroshi Yamada, Masaya Takemoto, Tohru Daikoku, Kimiyasu Shiraki

    Viral immunology   30 ( 7 )   500 - 507   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT, INC  

    Intravenous immunoglobulin (IVIG) is used to treat or prevent severe viral infection, especially cytomegalovirus (CMV) infections. IVIG was characterized to understand its interaction with CMV-infected cells. IVIG retarded CMV spread and reduced virus yields depending on the neutralizing (NT) antibody titer. Immediate early protein synthesis was reduced by IVIG in 3 to 15 h, and IVIG specifically reduced the ratio of 66/68k protein synthesis among immediate early proteins in an NT antibody-dependent manner between 4 and 8 h after infection, indicating that antigenic modulation of CMV-infected cells by IVIG reduced viral protein synthesis and virus production. The half-life of antibody bound to CMV-infected cells was 3.8 h. NT antibody titers to varicella-zoster virus (VZV) and CMV in IVIG were dose dependently absorbed by cells infected with VZV and CMV, respectively, but the antibody titers to CMV and VZV, respectively, were not affected. NT antibody in 0.3 mL of IVIG (15 mg) was specifically absorbed by 108 CMV-infected cells and 107 VZV-infected cells, suggesting that the NT antibody in IVIG might be inactivated by one-tenth of a similar volume of CMV-infected or VZV-infected cells. Various antiviral activities of IVIG may contribute to control and alleviation of CMV infection.

    DOI: 10.1089/vim.2016.0151

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  • Acyclovir-resistant herpes simplex virus 1 infection early after allogeneic hematopoietic stem cell transplantation with T-cell depletion 査読 国際誌

    Yu Akahoshi, Junya Kanda, Ayumu Ohno, Yusuke Komiya, Ayumi Gomyo, Jin Hayakawa, Naonori Harada, Kazuaki Kameda, Tomotaka Ugai, Hidenori Wada, Yuko Ishihara, Koji Kawamura, Kana Sakamoto, Miki Sato, Kiriko Terasako-Saito, Shun ichi Kimura, Misato Kikuchi, Hideki Nakasone, Shinichi Kako, Kimiyasu Shiraki, Yoshinobu Kanda

    Journal of Infection and Chemotherapy   23 ( 7 )   485 - 487   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We previously reported that oral low-dose acyclovir (200 mg/day) for the prevention of herpes simplex virus (HSV) infections after allogenic hematopoietic stem cell transplantation (HSCT) is effective without the emergence of acyclovir-resistant HSV infections. However, HSV infections are of significant concern because the number of allogeneic HSCT with T-cell depletion, which is a risk factor of the emergence of drug-resistant HSV infections, has been increasing. We experienced a 25-year-old female who received allogenic HSCT from an unrelated donor with 1-antigen mismatch using anti-thymocyte globulin. Despite acyclovir prophylaxis (200 mg/day), she developed the right palatal ulcer that was positive for HSV-1 specific antigen by fluorescent antibody on day 20 and developed new hypoglossal and tongue ulcers on day 33. Replacement of acyclovir with foscarnet improved her ulcers. We isolated 2 acyclovir-resistant and foscarnet-sensitive strains from the right palatal and hypoglossal ulcers, which had the same frame shift mutation in the thymidine kinase genes. The rate of proliferation of the isolate from the hypoglossal ulcer was faster than that from the right palatal ulcer in the plaque reduction assay. HSV strains that acquired acyclovir-resistant mutations at the right palatal ulcer with larger plaque might spread to the hypoglossal ulcer as the secondary site of infection because of better growth property. Second-line antiviral agents should be considered when we suspect treatment failure of HSV infection, especially in HSCT with T-cell depletion. Further studies are required whether low-dose acyclovir prophylaxis leads to the emergence of virological resistance.

    DOI: 10.1016/j.jiac.2017.02.001

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  • Detection of the BLV provirus from nasal secretion and saliva samples using BLV-CoCoMo-qPCR-2: Comparison with blood samples from the same cattle 査読 国際誌

    Yuan Yuan, Yuri Kitamura-Muramatsu, Susumu Saito, Hiroshi Ishizaki, Miwa Nakano, Satoshi Haga, Kazuhiro Matoba, Ayumu Ohno, Hironobu Murakami, Shin nosuke Takeshima, Yoko Aida

    Virus Research   210   248 - 254   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bovine leukemia virus (BLV) induces enzootic bovine leukosis, which is the most common neoplastic disease in cattle. Sero-epidemiological studies show that BLV infection occurs worldwide. Direct contact between infected and uninfected cattle is thought to be one of the risk factors for BLV transmission. Contact transmission occurs via a mixture of natural sources, blood, and exudates. To confirm that BLV provirus is detectable in these samples, matched blood, nasal secretion, and saliva samples were collected from 50 cattle, and genomic DNA was extracted. BLV-CoCoMo-qPCR-2, an assay developed for the highly sensitive detection of BLV, was then used to measure the proviral load in blood (n=50), nasal secretions (n=48), and saliva (n=47) samples. The results showed that 35 blood samples, 14 nasal secretion samples, and 6 saliva samples were positive for the BLV provirus. Matched blood samples from cattle that were positive for the BLV provirus (either in nasal secretion or saliva samples) were also positive in their blood. The proviral load in the positive blood samples was >14,000 (copies/1×105 cells). Thus, even though the proviral load in the nasal secretion and saliva samples was much lower (<380 copies/1×105 cells) than that in the peripheral blood, prolonged direct contact between infected and healthy cattle may be considered as a risk factor for BLV transmission.

    DOI: 10.1016/j.virusres.2015.08.013

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  • Risk factors associated with increased bovine leukemia virus proviral load in infected cattle in Japan from 2012 to 2014 査読 国際誌

    Ayumu Ohno, Shin nosuke Takeshima, Yuki Matsumoto, Yoko Aida

    Virus Research   210   283 - 290   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, a malignant B cell lymphoma. BLV has spread worldwide and causes serious problems. After infection, the BLV genome is integrated into the host DNA and can be amplified during periods of latency. We previously designed degenerate primers using the Coordination of Common Motifs (CoCoMo) algorithm to establish a new quantitative real-time PCR method (BLV-CoCoMo-qPCR-2) of measuring the proviral load of both known and novel BLV variants. Here, we aimed to examine the correlation between proviral load and risk factors for BLV infection, such as breeding systems, parousity, and colostrum feeding. Blood and serum samples were collected from 83 BLV-positive farms in 22 prefectures of Japan, and the BLV proviral load and anti-BLV antibody levels were measured. BLV was detected in 73.3% (1039/1,417) of cattle by BLV-CoCoMo-qPCR-2 and the provirus was detected in 93 of 1039 antibody-negative samples. The results showed that the proviral load increased with progression of lymphocytosis. Next, the risk factors associated with increasing BLV infection rate were examined along with any association with proviral load. The proviral load was higher in cattle with lymphocytosis than in healthy cattle, and higher in multiparous cows than in nulliparous cows. Finally, proviral loads were higher in contact breeding systems than in non-contact breeding systems. Taken together, these findings may help to formulate a plan for eliminating BLV from contaminated farms. This is the first nationwide study to estimate BLV proviral load in Japanese cattle.

    DOI: 10.1016/j.virusres.2015.08.020

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  • Detection and molecular characterization of bovine leukemia virus in Philippine cattle 査読 国際誌

    Meripet Polat, Ayumu Ohno, Shin nosuke Takeshima, Jiyun Kim, Mari Kikuya, Yuki Matsumoto, Claro Niegos Mingala, Misao Onuma, Yoko Aida

    Archives of Virology   160 ( 1 )   285 - 296   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4+ T-cell epitope, the CD8+ T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.

    DOI: 10.1007/s00705-014-2280-3

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  • Molecular evolution of the hemagglutinin and neuraminidase genes of pandemic (H1N1) 2009 influenza viruses in Sendai, Japan, during 2009-2011 査読 国際誌

    Irona Khandaker, Akira Suzuki, Taro Kamigaki, Kentaro Tohma, Takashi Odagiri, Takashi Okada, Ayumu Ohno, Kanako Otani, Rumi Sawayama, Kazuhisa Kawamura, Michiko Okamoto, Hitoshi Oshitani

    Virus Genes   47 ( 3 )   456 - 466   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Abstract: Analyzing the evolutionary pattern of the influenza A(H1N1)pdm09 strain in different regions is important for understanding its diversification. We therefore conducted this study to elucidate the genetic variability and molecular evolution of the influenza A(H1N1)pdm09 strains that circulated during the 2009-2010 and 2010-2011 influenza seasons in Sendai, Japan. Nasopharyngeal swab specimens were collected from patients with influenza-like illnesses who visited outpatient clinics in Sendai City, Japan, from September 2009 to April 2011. A total of 75 isolates were selected from September 2009 to April 2011 to analyze the genetic changes in the entire hemagglutinin 1 (HA1) segment of the HA gene and the neuraminidase (NA) gene based on sequence analysis. Bayesian coalescent Markov chain Monte Carlo analyses of HA1 and NA gene sequences were performed for further analysis. High sequence identities were observed for HA1 and NA in influenza A(H1N1)pdm09, displaying 99.06 and 99.33 % nucleotide identities, respectively, with the A(H1N1)pdm09 vaccine strain A/California/07/2009. The substitution rates of nucleotides for HA1 in the 2009-2010 and 2010-2011 were 1.5 × 10-3 and 1.6 × 10 -3 substitutions per site per year, respectively. Phylogenetic tree analysis demonstrated that Sendai isolates were clustered into global clade 7, which is characterized by an S203T mutation in the HA1 gene. Moreover, two distinct circulation clusters were present in the 2010-2011 season. Mutations were present in antigenic or receptor-binding domains of the HA1 segment, including A141V, S143G, S183P, S185T, and S203T. The Bayesian skyline plot model illustrated a steady rate for the maintenance of genetic diversity, followed by a slight increase in the later part of the 2010-2011 season. Selection analysis revealed that the HA1 (position 197) and NA (position 46) sites were under positive selection; however, no known mutation conferring resistance to NA inhibitors such as H275Y was observed. The effect on control of the influenza A(H1N1)pdm09 virus, including vaccine strain selection, requires continuous monitoring of the strain by genetic surveillance. © 2013 The Author(s).

    DOI: 10.1007/s11262-013-0980-5

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  • Genetic characterization of human respiratory syncytial virus detected in hospitalized children in the Philippines from 2008 to 2012 査読 国際誌

    Ayumu Ohno, Akira Suzuki, Socorro Lupisan, Hazel Galang, Lydia Sombrero, Rapunzel Aniceto, Michiko Okamoto, Mariko Saito, Naoko Fuji, Hirono Otomaru, Chandra Nath Roy, Dai Yamamoto, Raita Tamaki, Remigio Olveda, Hitoshi Oshitani

    Journal of Clinical Virology   57 ( 1 )   59 - 65   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: Human respiratory syncytial virus (HRSV) is the leading cause of acute lower respiratory tract infection in infants and young children. However, molecular characteristic of HRSV is still unknown in the Philippines. Objective: To describe the molecular epidemiology of circulating HRSV detected in the Philippines. Study design: From May 2008 to April 2012, nasopharyngeal swabs were collected from infants and children aged between 7 days and 14 years who were hospitalized with severe pneumonia. HRSV was detected by nested PCR targeting M2 gene, and C-terminus of the G gene was sequenced for phylogenetic analysis. Result: Out of total 2150 samples, 19.3% (n= 415) were positive for HRSV, and 65.0% of them (n= 270) were identified as HRSV-A and 35.0% (n= 145) as HRSV-B. There were two major HRSV outbreaks: between June 2008 and February 2009, and between June and March 2012. Majority of HRSV strains detected during the former outbreak were HRSV-A (97.5%, 203/208) whereas during the later outbreak, both HRSV-A (54/158, 34.2%) and HRSV-B (104/158, 65.8%) were detected. All HRSV-A strains were classified as genotype NA1 and all HRSV-B as genotype BA, which had 60-nucleotide duplication in secondary hypervariable region of the G gene. Among HRSV-B positive samples, there were 2 distinct clusters with unique amino acid changes and low homology in compared to other strains in BA, suggesting emergence of new variant of HRSV-B. Conclusion: The study provides an overview of the genetic variation in circulating HRSV viruses in the Philippines along with identification of possibly a novel variant of HRSV-B. © 2013 Elsevier B.V.

    DOI: 10.1016/j.jcv.2013.01.001

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MISC

  • 古細菌ゲノムのSpike-inを利用した定量的16Sメタゲノム解析法の評価(Evaluation of quantitative 16S metagenomic analysis using spike-in archaeal genome)

    大野 歩, 高橋 麻乃, 羽原 拓哉, Kryukov Kirill, 中川 草, 今西 規

    日本細菌学雑誌   76 ( 1 )   67 - 67   2021年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • Propidium monoazideとnanopore DNAシークエンサーを用いた薬剤耐性細菌の迅速なプロファイリング法(Rapid profiling of drug-resistant bacteria using propidium monoazide and a nanopore DNA sequencer)

    大野 歩, 梅澤 和夫, 浅井 さとみ, Kryukov Kirill, 中川 草, 宮地 勇人, 今西 規

    日本細菌学雑誌   75 ( 1 )   69 - 69   2020年1月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • Nanopore DNA Sequencerを用いた迅速なウイルス検出法の検討

    大野歩, KRYUKOV Kirill, 中川草, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020年

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  • MinIONを用いたDNA型判定の試み(その3)

    麻生川稔, 麻生川稔, 大野歩, 中川草, 落合恵理子, 大澤資樹, 杉澤正俊, 今西規

    日本法科学技術学会誌   25 ( Supplement )   2020年

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  • 感染症医療の未来を拓く新たな検査 ナノポアDNAシークエンサーを用いた迅速な細菌同定法

    大野歩, 中川草, KRYUKOV Kirill, KRYUKOV Kirill, 今西規

    臨床化学   49 ( 4 )   265 - 270   2020年

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    記述言語:日本語   出版者・発行元:(一社)日本臨床化学会  

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  • Propidium monoazideを用いたゲノム解析による迅速な薬剤耐性菌の同定

    大野歩, 梅澤和夫, KRYUKOV Kirill, 中川草, 浅井さとみ, 宮地勇人, 今西規

    日本ゲノム微生物学会年会要旨集   13th   2019年

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  • ナノポアDNAシークエンサーを用いた薬剤耐性菌同定技術

    大野歩, 梅澤和夫, KRYUKOV Kirill, 中川草, 浅井さとみ, 宮地勇人, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019年

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  • MinIONを用いたDNA型判定の試み(その2)

    麻生川稔, 麻生川稔, 大野歩, 中川草, 落合恵理子, 片平泰弘, 須藤恵美, 大澤資樹, 杉澤正俊, 今西規

    日本法科学技術学会誌   24 ( Supplement )   2019年

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  • MinIONを用いたDNA型判定の試み(その1)

    麻生川稔, 麻生川稔, 杉澤正俊, 大野歩, 中川草, 今西規

    日本法科学技術学会誌   23 ( Supplement )   2018年

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  • 生菌抽出法による薬剤耐性菌の迅速なプロファイリング技術の開発

    大野歩, 梅澤和夫, KRYUKOV Kirill, 中川草, 浅井さとみ, 宮地勇人, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   41st   2018年

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  • 次世代シークエンサーを用いたヒト膣内細菌叢の多様性解析

    須藤恵美, 大野歩, KRYUKOV Kirill, 宮澤麻里子, 信田政子, 三上幹男, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   41st   2018年

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  • ホルスタイン種における牛白血病抵抗性・感受性を規定するウシ主要組織適合遺伝子複合体クラスIIアリルの同定

    竹嶋伸之輔, 大野歩, 間陽子

    日本獣医師会獣医学術学会年次大会講演要旨集   2017   2018年

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  • 全国調査に基づく乳牛および肉牛の牛白血病プロウイルス量の制御に関わる主要組織適合抗原クラスII遺伝子の検出

    竹嶋伸之輔, 大野歩, 菊谷真理, 松本有生, 小原潤子, 間陽子

    日本獣医学会学術集会講演要旨集   159th   392 - 392   2016年

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • Epidemiological features of BLV infection in Japan from 2012 to 2013

    Ayumu Ohno, Shin-nosuke Takeshima, Mari Kikuya, Yuki Matsumoto, Yoko Aida

    RETROVIROLOGY   12   2015年8月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:BIOMED CENTRAL LTD  

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  • Epitope mapping of CD8+T cells on bovine leukemia virus Gag, Env and Tax protein in cattle with different bovine MHC DRB3 alleles

    Lanlan Bai, Shin-nosuke Takeshima, Ayumu Ohno, Yuki Matsumoto, Emiko Isogai, Junko Kohara, Yoko Aida

    RETROVIROLOGY   12   2015年8月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:BIOMED CENTRAL LTD  

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  • 国内の牛白血病ウイルス感染牛において,BLV-CoCoMo-qPCRにより検出されたプロウイルス量の解析

    大野歩, 竹嶋伸之輔, 間陽子

    日本獣医学会学術集会講演要旨集   158th   335 - 335   2015年

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • BLV-CoCoMo Direct PCR法を用いた全血からの牛白血病ウイルスの検出法の開発と実証試験

    綿貫園子, 竹嶋伸之輔, 大野歩, 若松敏枝, 的場和弘, 石崎宏, 間陽子

    日本獣医学会学術集会講演要旨集   158th   335 - 335   2015年

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  • フィリピンの牛白血病ウイルスの検出と分子性状解析(Detection and molecular characterization of bovine leukemia virus from the Philippines)

    Polat Meripet, 大野 歩, 竹嶋 伸之輔, 菊谷 真理, 松本 有生, Mingala Claro N., 小沼 操, 間 陽子

    日本獣医学会学術集会講演要旨集   157回   407 - 407   2014年8月

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    記述言語:英語   出版者・発行元:(公社)日本獣医学会  

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  • 牛白血病ウイルスのCD8+T細胞エピトープのマッピング-異なる主要組織適合遺伝子複合体(MHC)を有するウシを用いた解析-

    BAI Lanlan, BAI Lanlan, 竹嶋伸之輔, 大野歩, 松本有生, 磯貝恵美子, 小原潤子, 間陽子

    日本ウイルス学会学術集会プログラム・抄録集   62nd   2014年

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  • 国内の牛白血病ウイルス感染牛において,BLV-CoCoMo-qPCRにより検出されたプロウイルス量の解析

    大野歩, 竹嶋伸之輔, 菊谷真理, 松本有生, 間陽子

    日本ウイルス学会学術集会プログラム・抄録集   62nd   2014年

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  • BLV-CoCoMo-qPCRを用いた国内の牛白血病ウイルス感染牛のプロウイルス量の解析

    大野歩, 竹嶋伸之輔, 菊谷真理, 松本有生, 間陽子

    日本獣医学会学術集会講演要旨集   157th   424 - 424   2014年

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • 異なるBoLA-DRB3アリル遺伝子を保有するウシにおける牛白血病ウイルスCD8+ T細胞エピトープのマッピング

    BAI Lanlan, BAI Lanlan, 竹嶋伸之輔, 大野歩, 松本有生, 磯貝恵美子, 小原潤子, 間陽子

    日本獣医学会学術集会講演要旨集   157th   408 - 408   2014年

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • 日本におけるBLV-CoCoMo-qPCRを用いた牛白血病感染牛のプロウイルス量の調査

    大野歩, 竹嶋伸之輔, 松本有生, 間陽子

    日本ウイルス学会学術集会プログラム・抄録集   61st   2013年

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  • フィリピン・レイテ島における小児重症肺炎の疫学研究

    鈴木陽, 大野歩, 藤直子, 古瀬祐気

    日本小児科学会雑誌   114 ( 2 )   217 - 217   2010年

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    記述言語:日本語   出版者・発行元:(公社)日本小児科学会  

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  • 2008~2009年フィリピン・レイテ島タクロバンにおいて小児重症肺炎患者から検出されたRSウイルス

    大野歩, 鈴木陽, 藤直子, 古瀬祐気, 玉記雷太, 齋藤麻理子, 押谷仁

    感染症学雑誌   84 ( 5 )   290 - 290   2010年

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    記述言語:日本語   出版者・発行元:(一社)日本感染症学会  

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  • 2009/2010シーズンに仙台市内で流行したRSウイルスの解析

    大野歩, 鈴木陽, 押谷仁, 川村和久

    外来小児科   13 ( 3 )   381 - 381   2010年

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    記述言語:日本語   出版者・発行元:(一社)日本外来小児科学会  

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  • フィリピン・レイテ島における小児重症肺炎の疫学調査

    鈴木陽, 古瀬祐気, 藤直子, 大野歩, 玉記雷太, 齋藤麻理子, 押谷仁

    日本小児科学会雑誌   113 ( 12 )   1876 - 1876   2009年

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    記述言語:日本語   出版者・発行元:(公社)日本小児科学会  

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  • 下垂体腺腫の機能分化におけるNotchシグナル伝達の解析

    江頭登, 大野歩, 竹井麻生, 梶谷華子, 宮腰隆史, 竹腰進, 寺本明, 長村義之

    日本内分泌学会雑誌   83 ( 1 )   173 - 173   2007年

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    記述言語:日本語   出版者・発行元:(一社)日本内分泌学会  

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  • ヒト下垂体腺腫におけるNotchシグナル伝達の解析

    江頭登, 大野歩, 竹井麻生, 梶谷華子, 宮腰隆史, 竹腰進, 寺本明, 長村義之

    日本病理学会会誌   96 ( 1 )   299 - 299   2007年

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    記述言語:日本語   出版者・発行元:(一社)日本病理学会  

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  • ヒト下垂体・腺腫におけるNOTCHシグナル分子の発現解析

    江頭登, 大野歩, 竹井麻生, 竹腰進, 寺本明, 長村義之

    日本内分泌学会雑誌   83 ( 2 )   402 - 402   2007年

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共同研究・競争的資金等の研究

  • 次世代シークエンサーを用いた鼻腔内混合感染の実態調査および重症化リスクの検証

    研究課題/領域番号:20K17196  2020年04月 - 2022年03月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    大野 歩

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    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

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