Updated on 2022/12/17

写真a

 
Ohno Ayumu
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Special-Appointment Assistant Professor
Position
Special-Appointment Assistant Professor
External link

Degree

  • 博士(医学) ( 2013.3   東北大学 )

Research Areas

  • Life Science / Molecular biology

  • Life Science / Bacteriology

  • Life Science / Virology

Education

  • 東北大学大学院 医学系研究科 博士課程    

    2009.4 - 2013.3

      More details

  • 金沢大学大学院 医学系研究科 修士課程    

    2007.4 - 2009.3

      More details

  • 東海大学 開発工学部 生物工学科    

    2003.4 - 2007.3

      More details

Research History

  • Collaborative Research Center of OKAYAMA University for Infectious Diseases in India at NICED

    2021.11

      More details

  • Okayama University   Graduate School of Medicine , Dentistry and Pharmaceutical Sciences   Assistant Professor

    2021.9

      More details

  • Tokai University   医学部基礎医学系分子生命科学情報生物医学研究室   Researcher

    2017.9 - 2021.8

      More details

  • University of Toyama   Graduate School of Medicine and Pharmaceutical Sciences for Research   Researcher

    2017.4 - 2017.7

      More details

  • University of Toyama   Graduate School of Medicine and Pharmaceutical Sciences for Research   Assistant Professor

    2016.4 - 2017.3

      More details

  • University of Toyama   Graduate School of Medicine and Pharmaceutical Sciences for Research   Researcher

    2015.8 - 2016.3

      More details

  • RIKEN   分子ウイルス学特別研究ユニット   Research fellow

    2013.6 - 2015.7

      More details

▼display all

Professional Memberships

 

Papers

  • Burden of Shigella in South Asia: a systematic review and meta-analysis. International journal

    Basilua Andre Muzembo, Kei Kitahara, Debmalya Mitra, Ayumu Ohno, Januka Khatiwada, Shanta Dutta, Shin-Ichi Miyoshi

    Journal of travel medicine   2022.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Shigella remains one of the most common causes of diarrhoea in South Asia. Current estimates of the prevalence of Shigella are critical for guiding control measures. We estimated the prevalence of Shigella species and serogroups in South Asia. METHODS: We performed a systematic review using PubMed, EMBASE, Google Scholar, and Web of Science for peer-reviewed studies published between 2000 and June 19, 2022. We also manually searched the reference lists of the reviewed studies to identify additional studies. We included studies that detected the presence of Shigella in stool by culture or polymerase chain reaction (PCR). Studies associated with outbreaks were excluded. Two investigators independently reviewed the studies, extracted the data, and performed quality assessment. A random-effects meta-analysis was performed to determine the pooled prevalence of Shigella. RESULTS: Our search yielded 5707 studies, of which 91 studies from five South Asian countries were included in the systematic review, 79 in the meta-analysis of Shigella prevalence and 63 in the meta-analysis of Shigella serogroups prevalence. The pooled prevalence of Shigella was 7% (95% CI: 6-7%), with heterogeneity (I2 = 98.7; p < 0.01). The prevalence of Shigella was higher in children aged < 5 years (10%; 95% CI: 8-11%), in rural areas (12%; 95% CI: 10-14%), and in studies using PCR (15%; 95% CI: 11-19%). Shigella flexneri (58%) was the most abundant serogroup, followed by Shigella sonnei (19%), Shigella boydii (10%), and Shigella dysenteriae (9%). S. flexneri 2a was the most frequently isolated serotype (36%), followed by serotype 3a (12%), serotype 6 (12%), and serotype 1b (6%). The prevalence of non-typeable Shigella was 10.0%. CONCLUSIONS: Although the prevalence of Shigella in South Asia remains generally high, it varies by age group and geographical area, with data lacking in some countries. Effective Shigella vaccines would be advantageous for both endemic communities and travellers.

    DOI: 10.1093/jtm/taac132

    PubMed

    researchmap

  • Colonization with extended-spectrum beta-lactamase-producing Escherichia coli and traveler's diarrhea attack rates among travelers to India: a systematic review and meta-analysis. International journal

    Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Keinosuke Okamoto, Shin-Ichi Miyoshi

    Tropical diseases, travel medicine and vaccines   8 ( 1 )   22 - 22   2022.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: India is an attractive destination for travelers. Unfortunately, numerous reports exist on traveler's diarrhea (TD) and fecal colonization with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) among international travelers visiting India. Here, we systematically reviewed studies published on the acquisition of ESBL-EC and TD attack rates among international visitors to India. METHODS: Design: Systematic review and meta-analysis. A systematic search was performed using Google Scholar, PubMed, EMBASE, Web of Science, and gray literature from 2000 to December 2021, for studies containing data for ESBL-EC acquisition or TD experience related to a trip to India. Random effects models were used to compute the prevalence of ESBL-EC acquisition and TD attack. RESULTS: The literature search yielded a total of 5023 records. Of these, 31 met our inclusion criteria for systematic review and only 17 could be meta-analyzed (9 for TD, and 8 for ESBL-EC). The overall pooled attack rate of TD was 39% (95% confidence interval, CI: 25-53%). In studies where travelers' memory was used to diagnose TD, the pooled attack rate of TD was slightly higher (42%, 95% CI: 21-64%) compared to those where TD was objectively documented (33%, 95% CI: 17-49%). There were significant risks to be colonized with ESBL-EC among the travelers who experienced TD. The pooled rate of ESBL-EC colonization was 72% (CI: 67-78%). Most ESBL-EC produced CTX-M-15 enzyme. Furthermore, most of the travelers who acquired ESBL-EC were from highly industrialized countries recruited from travel clinics: Canada (n = 80), Germany (n = 69), Netherlands (n = 20), Sweden (n = 18), Japan (n = 10), Finland (n = 8), USA (n = 7), Spain (n = 5), and Denmark (n = 3). CONCLUSIONS: TD pooled attack rate and ESBL-EC acquisition among international travelers visiting India were high in this study. However, we cannot make generalizations based upon this TD pooled attack rate for the current situation, due to a lack of current data. Our study highlights that travelers should be advised on TD to ensure that they do not disregard the risk of contracting TD and be better prepared as a result. It also illustrates the importance of international travel in acquiring antibiotic-resistant Escherichia coli.

    DOI: 10.1186/s40794-022-00179-1

    PubMed

    researchmap

  • Rapid diagnostic tests versus RT-PCR for Ebola virus infections: a systematic review and meta-analysis. International journal

    Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Ngangu Patrick Ntontolo, Nlandu Roger Ngatu, Keinosuke Okamoto, Shin-Ichi Miyoshi

    Bulletin of the World Health Organization   100 ( 7 )   447 - 458   2022.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To evaluate the clinical accuracy of rapid diagnostic tests for the detection of Ebola virus. METHODS: We searched MEDLINE®, Embase® and Web of Science for articles published between 1976 and October 2021 reporting on clinical studies assessing the performance of Ebola virus rapid diagnostic tests compared with reverse transcription polymerase chain reaction (RT-PCR). We assessed study quality using the QUADAS-2 criteria. To estimate the pooled sensitivity and specificity of these rapid diagnostic tests, we used a bivariate random-effects meta-analysis. FINDINGS: Our search identified 113 unique studies, of which nine met the inclusion criteria. The studies were conducted in the Democratic Republic of the Congo, Guinea, Liberia and Sierra Leone and they evaluated 12 rapid diagnostic tests. We included eight studies in the meta-analysis. The pooled sensitivity and specificity of the rapid tests were 86% (95% confidence interval, CI: 80-91) and 95% (95% CI: 91-97), respectively. However, pooled sensitivity decreased to 83% (95% CI: 77-88) after removing outliers. Pooled sensitivity increased to 90% (95% CI: 82-94) when analysis was restricted to studies using the RT-PCR from altona Diagnostics as gold standard. Pooled sensitivity increased to 99% (95% CI: 67-100) when the analysis was restricted to studies using whole or capillary blood specimens. CONCLUSION: The included rapid diagnostic tests did not detect all the Ebola virus disease cases. While the sensitivity and specificity of these tests are moderate, they are still valuable tools, especially useful for triage and detecting Ebola virus in remote areas.

    DOI: 10.2471/BLT.21.287496

    PubMed

    researchmap

  • Long-Term Kinetics of Serological Antibodies against Vibrio cholerae Following a Clinical Cholera Case: A Systematic Review and Meta-Analysis. International journal

    Basilua Andre Muzembo, Kei Kitahara, Debmalya Mitra, Ayumu Ohno, Shin-Ichi Miyoshi

    International journal of environmental research and public health   19 ( 12 )   2022.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Approximately 2.9 million people worldwide suffer from cholera each year, many of whom are destitute. However, understanding of immunity against cholera is still limited. Several studies have reported the duration of antibodies following cholera; however, systematic reviews including a quantitative synthesis are lacking. OBJECTIVE: To meta-analyze cohort studies that have evaluated vibriocidal, cholera toxin B subunit (CTB), and lipopolysaccharide (LPS) antibody levels following a clinical cholera case. METHODS: Design: Systematic review and meta-analysis. We searched PubMed and Web of science for studies assessing antibodies against Vibrio cholerae in cohorts of patients with clinical cholera. Two authors independently extracted data and assessed the quality of included studies. Random effects models were used to pool antibody titers in adults and older children (aged ≥ 6 years). In sensitivity analysis, studies reporting data on young children (2-5 years) were included. RESULTS: Nine studies met our inclusion criteria for systematic review and seven for meta-analysis. The pooled mean of vibriocidal antibody titers in adults and older children (aged ≥ 6 years) was 123 on day 2 post-symptom onset, which sharply increased on day 7 (pooled mean = 6956) and gradually waned to 2247 on day 30, 578 on day 90, and 177 on day 360. Anti-CTB IgA antibodies also peaked on day 7 (pooled mean = 49), followed by a rapid decrease on day 30 (pooled mean = 21), and further declined on day 90 (pooled mean = 10), after which it plateaued from day 180 (pooled mean = 8) to 360 (pooled mean = 6). Similarly, anti-CTB IgG antibodies peaked in early convalescence between days 7 (pooled mean = 65) and 30 (pooled mean = 69), then gradually waned on days 90 (pooled mean = 42) and 180 (pooled mean = 30) and returned to baseline on day 360 (pooled mean = 24). Anti-LPS IgA antibodies peaked on day 7 (pooled mean = 124), gradually declined on day 30 (pooled mean = 44), which persisted until day 360 (pooled mean = 10). Anti LPS IgG antibodies peaked on day 7 (pooled mean = 94). Thereafter, they decreased on day 30 (pooled mean = 85), and dropped further on days 90 (pooled mean = 51) and 180 (pooled mean = 47), and returned to baseline on day 360 (pooled mean = 32). Sensitivity analysis including data from young children (aged 2-5 years) showed very similar findings as in the primary analysis. CONCLUSIONS: This study confirms that serological antibody (vibriocidal, CTB, and LPS) titers return to baseline levels within 1 year following clinical cholera, i.e., before the protective immunity against subsequent cholera wanes. However, this decay should not be interpreted as waning immunity because immunity conferred by cholera against subsequent disease lasts 3-10 years. Our study provides evidence for surveillance strategies and future research on vaccines and also demonstrates the need for further studies to improve our understanding of immunity against cholera.

    DOI: 10.3390/ijerph19127141

    PubMed

    researchmap

  • Cholera Outbreaks in India, 2011-2020: A Systematic Review. International journal

    Basilua Andre Muzembo, Kei Kitahara, Anusuya Debnath, Ayumu Ohno, Keinosuke Okamoto, Shin-Ichi Miyoshi

    International journal of environmental research and public health   19 ( 9 )   2022.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Fecal contamination of water sources and open defecation have been linked to cholera outbreaks in India. However, a systematic review on the drivers responsible for these outbreaks has yet to be published. Here, we systematically review the published literature on cholera outbreaks in India between 2011 and 2020. We searched studies in English in three databases (MEDLINE, EMBASE, and Web of Science) and the Integrated Disease Surveillance Program that tracks cholera outbreaks throughout India. Two authors independently extracted data and assessed the quality of the included studies. Quantitative data on the modes of transmission reviewed in this study were assessed for any change over time between 2011-2015 and 2016-2020. Our search retrieved 10823 records initially, out of which 81 full-text studies were assessed for eligibility. Among these 81 studies, 20 were eligible for inclusion in this review. There were 565 reported outbreaks between 2011 and 2020 that led to 45,759 cases and 263 deaths. Outbreaks occurred throughout the year; however, they exploded with monsoons (June through September). In Tamil Nadu, a typical peak of cholera outbreaks was observed from December to January. Seventy-two percent (33,089/45,759) of outbreak-related cases were reported in five states, namely Maharashtra, West Bengal, Punjab, Karnataka, and Madhya Pradesh. Analysis of these outbreaks highlighted the main drivers of cholera including contaminated drinking water and food, inadequate sanitation and hygiene (including open defecation), and direct contact between households. The comparison between 2011-2015 and 2016-2020 showed a decreasing trend in the outbreaks that arose due to damaged water pipelines. Many Indians still struggle with open defecation, sanitation, and clean water access. These issues should be addressed critically. In addition, it is essential to interrupt cholera short-cycle transmission (mediated by households, stored drinking water and foodstuffs) during an outbreak. As cholera is associated with deprivation, socio-economic development is the only long-term solution.

    DOI: 10.3390/ijerph19095738

    PubMed

    researchmap

  • Regression tree analysis of the relationship between the concentrations of antimicrobial components and the microbiota of normal milk from dairy cows

    Yasunori SHINOZUKA, Naoki SUZUKI, Sohei KANEKO, Kazuhiro KAWAI, Tomomi KURUMISAWA, Yuko SHIMIZU, Tadashi IMANISHI, Ayumu OHNO, Mano TAKAHASHI, Naoki ISOBE

    Journal of Veterinary Medical Science   2022

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Veterinary Science  

    DOI: 10.1292/jvms.21-0541

    researchmap

  • Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer International journal

    Ayumu Ohno, Kazuo Umezawa, Satomi Asai, Kirill Kryukov, So Nakagawa, Hayato Miyachi, Tadashi Imanishi

    Scientific Reports   11 ( 1 )   3436 - 3436   2021.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Spread of drug-resistant bacteria is a serious problem worldwide. We thus designed a new sequence-based protocol that can quickly identify bacterial compositions of clinical samples and their drug-resistance profiles simultaneously. Here we utilized propidium monoazide (PMA) that prohibits DNA amplifications from dead bacteria, and subjected the original and antibiotics-treated samples to 16S rRNA metagenome sequencing. We tested our protocol on bacterial mixtures, and observed that sequencing reads derived from drug-resistant bacteria were significantly increased compared with those from drug-sensitive bacteria when samples were treated by antibiotics. Our protocol is scalable and will be useful for quickly profiling drug-resistant bacteria.

    DOI: 10.1038/s41598-021-82903-z

    Scopus

    PubMed

    researchmap

  • Cholera Rapid Diagnostic Tests for the Detection of Vibrio cholerae O1: An Updated Meta-Analysis. International journal

    Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Anusuya Debnath, Keinosuke Okamoto, Shin-Ichi Miyoshi

    Diagnostics (Basel, Switzerland)   11 ( 11 )   2021.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The rapid diagnosis of cholera contributes to adequate outbreak management. This meta-analysis assesses the diagnostic accuracy of cholera rapid tests (RDTs) to detect Vibrio cholerae O1. METHODS: Systematic review and meta-analysis. We searched four databases (Medline, EMBASE, Google Scholar, and Web of Science up to 8 September 2021) for studies that evaluated cholera RDTs for the detection of V. cholerae O1 compared with either stool culture or polymerase chain reaction (PCR). We assessed the studies' quality using the QUADAS-2 criteria. In addition, in this update, GRADE approach was used to rate the overall certainty of the evidence. We performed a bivariate random-effects meta-analysis to calculate the pooled sensitivity and specificity of cholera RDTs. RESULTS: Overall, 20 studies were included in this meta-analysis. Studies were from Africa (n = 11), Asia (n = 7), and America (Haiti; n = 2). They evaluated eight RDTs (Crystal VC-O1, Crystal VC, Cholkit, Institut Pasteur cholera dipstick, SD Bioline, Artron, Cholera Smart O1, and Smart II Cholera O1). Using direct specimen testing, sensitivity and specificity of RDTs were 90% (95% CI, 86 to 93) and 86% (95% CI, 81 to 90), respectively. Cholera Sensitivity was higher in studies conducted in Africa [92% (95% CI, 89 to 94)] compared with Asia [82% (95% CI, 77 to 87)]. However, specificity [83% (95% CI, 71 to 91)] was lower in Africa compared with Asia [90% (95% CI, 84 to 94)]. GRADE quality of evidence was estimated as moderate. CONCLUSIONS: Against culture or PCR, current cholera RDTs have moderate sensitivity and specificity for detecting Vibrio cholerae O1.

    DOI: 10.3390/diagnostics11112095

    PubMed

    researchmap

  • Examination of the microbiota of normal cow milk using MinIONTM nanopore sequencing.

    Yasunori Shinozuka, Kazuhiro Kawai, Tomomi Kurumisawa, Yuko Shimizu, Tadashi Imanishi, Ayumu Ohno, Mano Takahashi, Sohei Kaneko, Naoki Suzuki

    The Journal of veterinary medical science   83 ( 11 )   1620 - 1627   2021.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The aim of this study was to evaluate the microbiota of normal milk in dairy cows and their relationship with host factors, such as the age of the cow (Age), somatic cell counts in milk (SCCs), and days in milk (DIM). We investigated 48 milk samples from 22 cows with no systemic or local clinical signs using MinIONTM nanopore sequencing for a 16S rRNA gene amplicon. Bacterial richness was positively correlated with the DIM (P=0.043), and both the Shannon-Wiener Index and Simpson's Index, which are metrics of alpha-diversity, were also significantly positively correlated with the SCC (P<0.001). The composition ratios of both Actinobacteria at the phylum level and Kocuria spp. at the genus level in the milk microbiota were significantly correlated with the SCC (P<0.001 and P<0.001, respectively). In the beta-diversity test, the one-way analysis of similarities test showed a significant difference (P=0.0051) between the low- and high-SCC groups. This study clarified that the composition of the normal milk microbiota in this herd was related to the SCC. It also raised the possibility of variations in bacterial genera in the normal milk microbiota between the low- and high-SCC groups. However, to clarify the actual condition of the milk microbiota and to elucidate the relationship with the SCC, it is necessary to perform further analyses taking into account not only the relative abundance, but also the absolute abundance of microbes.

    DOI: 10.1292/jvms.21-0353

    PubMed

    researchmap

  • Human short tandem repeat identification using a nanopore-based DNA sequencer: a pilot study Reviewed International journal

    Minoru Asogawa, Ayumu Ohno, So Nakagawa, Eriko Ochiai, Yasuhiro Katahira, Megumi Sudo, Motoki Osawa, Masatoshi Sugisawa, Tadashi Imanishi

    Journal of Human Genetics   65 ( 1 )   21 - 24   2020.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Short tandem repeats (STRs) are repetitive DNA sequences that are highly polymorphic and widely used for personal identification in the field of forensic medicine. The standard method for determining the repeat number of STRs is capillary electrophoresis of PCR products; however, the use of DNA sequencing has increased because it can identify same-sized alleles with nucleotide substitutions (iso-alleles). In this study, we performed human STR genotyping using a portable nanopore-based DNA sequencer, the MinION, and evaluated its performance. Because the sequence quality obtained by MinION is considerably lower than those obtained with other DNA sequencers, we developed an original scoring scheme for judging the genotypes from MinION reads. Analysis of seven human samples for 21–45 STR loci yielded an average of 857 thousand reads per sample, and the accuracy of genotyping and iso-allele identification reached 75.7% and 82%, respectively. Although the accuracy is higher than that reported previously, further improvements are required before this method can be practically applied.

    DOI: 10.1038/s10038-019-0688-z

    Scopus

    PubMed

    researchmap

  • Bovine leukemia virus proviral load is more strongly associated with bovine major histocompatibility complex class II DRB3 polymorphism than with DQA1 polymorphism in Holstein cow in Japan Reviewed International journal

    Shin Nosuke Takeshima, Ayumu Ohno, Yoko Aida

    Retrovirology   16 ( 1 )   14 - 14   2019.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Bovine leukemia virus (BLV) causes enzootic bovine leukosis and is closely related to the human T-lymphotropic virus. Bovine major histocompatibility complex (BoLAs) are used extensively as markers of disease and immunological traits in cattle. For BLV diagnosis, proviral load is a major diagnosis index for the determination of disease progression and transmission risk. Therefore, we investigated the frequency of BoLA-DRB3 alleles, BoLA-DQA1 alleles, and haplotypes of BoLA class II isolated from the heads of 910 BLV-infected cows out of 1290 cows assessed from BLV-positive farms, in a nationwide survey from 2011 to 2014 in Japan. Our aim was to identify BoLA class II polymorphisms associated with the BLV proviral load in the Holstein cow. The study examined 569 cows with a high proviral load and 341 cows with a low proviral load. Using the highest odds ratio (OR) as a comparison index, we confirmed that BoLA-DRB3 was the best marker for determining which cow spread the BLV (OR 13.9 for BoLA-DRB3, OR 11.5 for BoLA-DQA1, and OR 6.2 for BoLA class II haplotype). In addition, DRB3002:01,009:02,012:01,014:01, and015:01 were determined as BLV provirus associated alleles. BoLA-DRB3002:01,009:02, and014:01 were determined as resistant alleles (OR > 1), and BoLA-DRB3012:01 and015:01 were determined as susceptible alleles (OR < 1). In this study, we showed that BoLA-DRB3 was a good marker for determining which cow spread BLV, and we found not only one resistant allele (BoLA-DRB3009:02), but also two other disease-resistant alleles and two disease-susceptible alleles. This designation of major alleles as markers of susceptibility or resistance can allow the determination of the susceptibility or resistance of most cows to disease. Overall, the results of this study may be useful in eliminating BLV from farms without having to separate cows into several cowsheds.

    DOI: 10.1186/s12977-019-0476-z

    Scopus

    PubMed

    researchmap

  • Rapid sequencing-based diagnosis of infectious bacterial species from meningitis patients in Zambia Reviewed International journal

    So Nakagawa, Shigeaki Inoue, Kirill Kryukov, Junya Yamagishi, Ayumu Ohno, Kyoko Hayashida, Ruth Nakazwe, Mox Kalumbi, Darlington Mwenya, Nana Asami, Chihiro Sugimoto, Mable M. Mutengo, Tadashi Imanishi

    Clinical and Translational Immunology   8 ( 11 )   e01087   2019.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Objectives: We have developed a portable system for the rapid determination of bacterial composition for the diagnosis of infectious diseases. Our system comprises of a nanopore technology-based sequencer, MinION, and two laptop computers. To examine the accuracy and time efficiency of our system, we provided a proof-of-concept for the detection of the causative bacteria of 11 meningitis patients in Zambia. Methods: We extracted DNA from cerebrospinal fluid samples of each patient and amplified the 16S rRNA gene regions. The sequencing library was prepared, and the sequenced reads were simultaneously processed for bacterial composition determination using the minimap2 software and the representative prokaryote genomes. Results: The sequencing results of four of the six culture-positive samples were consistent with those of conventional culture-based methods. The dominant bacterial species in each of these samples were identified from the sequencing data within only 3 min. Although the major bacterial species were also detected from the other two culture-positive samples and five culture-negative samples, their presence could not be confirmed. Moreover, as a whole, although the number of sequencing reads obtained within a short sequencing run was small, there was no change in the major bacterial species over time with prolonged sequencing. In addition, the processing time strongly correlated with the number of sequencing reads used for the analysis. Conclusion: Our results suggest that time-effective analysis could be achieved by determining the number of sequencing reads required for the rapid diagnosis of infectious bacterial species depending on the complexity of bacterial species in a sample.

    DOI: 10.1002/cti2.1087

    Scopus

    PubMed

    researchmap

  • Improved photocatalytic air cleaner with decomposition of aldehyde and aerosol-associated influenza virus infectivity in indoor air Reviewed

    Kimiyasu Shiraki, Hiroshi Yamada, Yoshihiro Yoshida, Ayumu Ohno, Teruo Watanabe, Takafumi Watanabe, Hiroyuki Watanabe, Hidemitsu Watanabe, Masao Yamaguchi, Fumio Tokuoka, Shigeatsu Hashimoto, Masakazu Kawamura, Norihisa Adachi

    Aerosol and Air Quality Research   17 ( 11 )   2901 - 2912   2017.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AAGR Aerosol and Air Quality Research  

    Air pollution caused by fine particulate matter (PM2.5), volatile organic compounds, and bioaerosols is a major environmental risk to health. We developed a photocatalytic air cleaner for reducing the pollution levels of indoor air; we improved the photocatalytic system by using UV-LED for the removal of acetaldehyde and PM2.5 and by reducing the weight and size of the system. The efficiency of photocatalysis depends on the surface area and materials. Therefore, we prepared a nanosized titanium dioxide (TiO2)-coated aluminum plate irradiated by UV-LED lamps (wavelength: 375 nm) as a photocatalytic air cleaner. Passing air continuously through a TiO2-coated aluminum plate (5 × 10 × 1 cm) under black light for 200 min decomposed 90% of 5 ppm acetaldehyde (12.4 µmol h–1) and generated two carbon dioxide molecules (25.43 µmol h–1) at a molar ratio of 1:2, indicating complete decomposition of acetaldehyde with high efficiency. This photocatalytic air cleaner was applied to the decomposition of acetaldehyde and inactivation and removal of aerosol-associated influenza virus. Acetaldehyde (20 ppm) in a 1-m3 cubic space was eliminated in 60 min at a half-life of 8 min. The aerosol-associated infectivity and the RNA genome of influenza virus A/PR/8/1934 (H1N1) produced by a nebulizer in a 779-L cubic space were eliminated within 7 min; however, they were detectable for up to 28 minutes when the functional photocatalytic air cleaner was not used. The presence of intermediate breakdown products of influenza virus indicated that the virus was broken down by photocatalysis. Thus, the photocatalytic air cleaner efficiently decomposed and eliminated organic chemicals, acetaldehyde, and aerosol-associated influenza virus infectivity and viral RNA, indicating that it can clean and detoxify the indoor air in a closed space for maintaining a safer environment.

    DOI: 10.4209/aaqr.2017.06.0220

    Scopus

    researchmap

  • Interaction of Immunoglobulin with Cytomegalovirus-Infected Cells. Reviewed International journal

    Nobuyasu Aiba, Atsuko Shiraki, Misako Yajima, Yukari Oyama, Yoshihiro Yoshida, Ayumu Ohno, Hiroshi Yamada, Masaya Takemoto, Tohru Daikoku, Kimiyasu Shiraki

    Viral immunology   30 ( 7 )   500 - 507   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARY ANN LIEBERT, INC  

    Intravenous immunoglobulin (IVIG) is used to treat or prevent severe viral infection, especially cytomegalovirus (CMV) infections. IVIG was characterized to understand its interaction with CMV-infected cells. IVIG retarded CMV spread and reduced virus yields depending on the neutralizing (NT) antibody titer. Immediate early protein synthesis was reduced by IVIG in 3 to 15 h, and IVIG specifically reduced the ratio of 66/68k protein synthesis among immediate early proteins in an NT antibody-dependent manner between 4 and 8 h after infection, indicating that antigenic modulation of CMV-infected cells by IVIG reduced viral protein synthesis and virus production. The half-life of antibody bound to CMV-infected cells was 3.8 h. NT antibody titers to varicella-zoster virus (VZV) and CMV in IVIG were dose dependently absorbed by cells infected with VZV and CMV, respectively, but the antibody titers to CMV and VZV, respectively, were not affected. NT antibody in 0.3 mL of IVIG (15 mg) was specifically absorbed by 108 CMV-infected cells and 107 VZV-infected cells, suggesting that the NT antibody in IVIG might be inactivated by one-tenth of a similar volume of CMV-infected or VZV-infected cells. Various antiviral activities of IVIG may contribute to control and alleviation of CMV infection.

    DOI: 10.1089/vim.2016.0151

    Web of Science

    PubMed

    researchmap

  • Acyclovir-resistant herpes simplex virus 1 infection early after allogeneic hematopoietic stem cell transplantation with T-cell depletion Reviewed International journal

    Yu Akahoshi, Junya Kanda, Ayumu Ohno, Yusuke Komiya, Ayumi Gomyo, Jin Hayakawa, Naonori Harada, Kazuaki Kameda, Tomotaka Ugai, Hidenori Wada, Yuko Ishihara, Koji Kawamura, Kana Sakamoto, Miki Sato, Kiriko Terasako-Saito, Shun ichi Kimura, Misato Kikuchi, Hideki Nakasone, Shinichi Kako, Kimiyasu Shiraki, Yoshinobu Kanda

    Journal of Infection and Chemotherapy   23 ( 7 )   485 - 487   2017.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    We previously reported that oral low-dose acyclovir (200 mg/day) for the prevention of herpes simplex virus (HSV) infections after allogenic hematopoietic stem cell transplantation (HSCT) is effective without the emergence of acyclovir-resistant HSV infections. However, HSV infections are of significant concern because the number of allogeneic HSCT with T-cell depletion, which is a risk factor of the emergence of drug-resistant HSV infections, has been increasing. We experienced a 25-year-old female who received allogenic HSCT from an unrelated donor with 1-antigen mismatch using anti-thymocyte globulin. Despite acyclovir prophylaxis (200 mg/day), she developed the right palatal ulcer that was positive for HSV-1 specific antigen by fluorescent antibody on day 20 and developed new hypoglossal and tongue ulcers on day 33. Replacement of acyclovir with foscarnet improved her ulcers. We isolated 2 acyclovir-resistant and foscarnet-sensitive strains from the right palatal and hypoglossal ulcers, which had the same frame shift mutation in the thymidine kinase genes. The rate of proliferation of the isolate from the hypoglossal ulcer was faster than that from the right palatal ulcer in the plaque reduction assay. HSV strains that acquired acyclovir-resistant mutations at the right palatal ulcer with larger plaque might spread to the hypoglossal ulcer as the secondary site of infection because of better growth property. Second-line antiviral agents should be considered when we suspect treatment failure of HSV infection, especially in HSCT with T-cell depletion. Further studies are required whether low-dose acyclovir prophylaxis leads to the emergence of virological resistance.

    DOI: 10.1016/j.jiac.2017.02.001

    Web of Science

    Scopus

    PubMed

    researchmap

  • Detection of the BLV provirus from nasal secretion and saliva samples using BLV-CoCoMo-qPCR-2: Comparison with blood samples from the same cattle Reviewed International journal

    Yuan Yuan, Yuri Kitamura-Muramatsu, Susumu Saito, Hiroshi Ishizaki, Miwa Nakano, Satoshi Haga, Kazuhiro Matoba, Ayumu Ohno, Hironobu Murakami, Shin nosuke Takeshima, Yoko Aida

    Virus Research   210   248 - 254   2015.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Bovine leukemia virus (BLV) induces enzootic bovine leukosis, which is the most common neoplastic disease in cattle. Sero-epidemiological studies show that BLV infection occurs worldwide. Direct contact between infected and uninfected cattle is thought to be one of the risk factors for BLV transmission. Contact transmission occurs via a mixture of natural sources, blood, and exudates. To confirm that BLV provirus is detectable in these samples, matched blood, nasal secretion, and saliva samples were collected from 50 cattle, and genomic DNA was extracted. BLV-CoCoMo-qPCR-2, an assay developed for the highly sensitive detection of BLV, was then used to measure the proviral load in blood (n=50), nasal secretions (n=48), and saliva (n=47) samples. The results showed that 35 blood samples, 14 nasal secretion samples, and 6 saliva samples were positive for the BLV provirus. Matched blood samples from cattle that were positive for the BLV provirus (either in nasal secretion or saliva samples) were also positive in their blood. The proviral load in the positive blood samples was >14,000 (copies/1×105 cells). Thus, even though the proviral load in the nasal secretion and saliva samples was much lower (<380 copies/1×105 cells) than that in the peripheral blood, prolonged direct contact between infected and healthy cattle may be considered as a risk factor for BLV transmission.

    DOI: 10.1016/j.virusres.2015.08.013

    Scopus

    PubMed

    researchmap

  • Risk factors associated with increased bovine leukemia virus proviral load in infected cattle in Japan from 2012 to 2014 Reviewed International journal

    Ayumu Ohno, Shin nosuke Takeshima, Yuki Matsumoto, Yoko Aida

    Virus Research   210   283 - 290   2015.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, a malignant B cell lymphoma. BLV has spread worldwide and causes serious problems. After infection, the BLV genome is integrated into the host DNA and can be amplified during periods of latency. We previously designed degenerate primers using the Coordination of Common Motifs (CoCoMo) algorithm to establish a new quantitative real-time PCR method (BLV-CoCoMo-qPCR-2) of measuring the proviral load of both known and novel BLV variants. Here, we aimed to examine the correlation between proviral load and risk factors for BLV infection, such as breeding systems, parousity, and colostrum feeding. Blood and serum samples were collected from 83 BLV-positive farms in 22 prefectures of Japan, and the BLV proviral load and anti-BLV antibody levels were measured. BLV was detected in 73.3% (1039/1,417) of cattle by BLV-CoCoMo-qPCR-2 and the provirus was detected in 93 of 1039 antibody-negative samples. The results showed that the proviral load increased with progression of lymphocytosis. Next, the risk factors associated with increasing BLV infection rate were examined along with any association with proviral load. The proviral load was higher in cattle with lymphocytosis than in healthy cattle, and higher in multiparous cows than in nulliparous cows. Finally, proviral loads were higher in contact breeding systems than in non-contact breeding systems. Taken together, these findings may help to formulate a plan for eliminating BLV from contaminated farms. This is the first nationwide study to estimate BLV proviral load in Japanese cattle.

    DOI: 10.1016/j.virusres.2015.08.020

    Scopus

    PubMed

    researchmap

  • Detection and molecular characterization of bovine leukemia virus in Philippine cattle Reviewed International journal

    Meripet Polat, Ayumu Ohno, Shin nosuke Takeshima, Jiyun Kim, Mari Kikuya, Yuki Matsumoto, Claro Niegos Mingala, Misao Onuma, Yoko Aida

    Archives of Virology   160 ( 1 )   285 - 296   2015.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4+ T-cell epitope, the CD8+ T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.

    DOI: 10.1007/s00705-014-2280-3

    Scopus

    PubMed

    researchmap

  • Molecular evolution of the hemagglutinin and neuraminidase genes of pandemic (H1N1) 2009 influenza viruses in Sendai, Japan, during 2009-2011 Reviewed International journal

    Irona Khandaker, Akira Suzuki, Taro Kamigaki, Kentaro Tohma, Takashi Odagiri, Takashi Okada, Ayumu Ohno, Kanako Otani, Rumi Sawayama, Kazuhisa Kawamura, Michiko Okamoto, Hitoshi Oshitani

    Virus Genes   47 ( 3 )   456 - 466   2013.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Abstract: Analyzing the evolutionary pattern of the influenza A(H1N1)pdm09 strain in different regions is important for understanding its diversification. We therefore conducted this study to elucidate the genetic variability and molecular evolution of the influenza A(H1N1)pdm09 strains that circulated during the 2009-2010 and 2010-2011 influenza seasons in Sendai, Japan. Nasopharyngeal swab specimens were collected from patients with influenza-like illnesses who visited outpatient clinics in Sendai City, Japan, from September 2009 to April 2011. A total of 75 isolates were selected from September 2009 to April 2011 to analyze the genetic changes in the entire hemagglutinin 1 (HA1) segment of the HA gene and the neuraminidase (NA) gene based on sequence analysis. Bayesian coalescent Markov chain Monte Carlo analyses of HA1 and NA gene sequences were performed for further analysis. High sequence identities were observed for HA1 and NA in influenza A(H1N1)pdm09, displaying 99.06 and 99.33 % nucleotide identities, respectively, with the A(H1N1)pdm09 vaccine strain A/California/07/2009. The substitution rates of nucleotides for HA1 in the 2009-2010 and 2010-2011 were 1.5 × 10-3 and 1.6 × 10 -3 substitutions per site per year, respectively. Phylogenetic tree analysis demonstrated that Sendai isolates were clustered into global clade 7, which is characterized by an S203T mutation in the HA1 gene. Moreover, two distinct circulation clusters were present in the 2010-2011 season. Mutations were present in antigenic or receptor-binding domains of the HA1 segment, including A141V, S143G, S183P, S185T, and S203T. The Bayesian skyline plot model illustrated a steady rate for the maintenance of genetic diversity, followed by a slight increase in the later part of the 2010-2011 season. Selection analysis revealed that the HA1 (position 197) and NA (position 46) sites were under positive selection; however, no known mutation conferring resistance to NA inhibitors such as H275Y was observed. The effect on control of the influenza A(H1N1)pdm09 virus, including vaccine strain selection, requires continuous monitoring of the strain by genetic surveillance. © 2013 The Author(s).

    DOI: 10.1007/s11262-013-0980-5

    Scopus

    PubMed

    researchmap

  • Genetic characterization of human respiratory syncytial virus detected in hospitalized children in the Philippines from 2008 to 2012 Reviewed International journal

    Ayumu Ohno, Akira Suzuki, Socorro Lupisan, Hazel Galang, Lydia Sombrero, Rapunzel Aniceto, Michiko Okamoto, Mariko Saito, Naoko Fuji, Hirono Otomaru, Chandra Nath Roy, Dai Yamamoto, Raita Tamaki, Remigio Olveda, Hitoshi Oshitani

    Journal of Clinical Virology   57 ( 1 )   59 - 65   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Background: Human respiratory syncytial virus (HRSV) is the leading cause of acute lower respiratory tract infection in infants and young children. However, molecular characteristic of HRSV is still unknown in the Philippines. Objective: To describe the molecular epidemiology of circulating HRSV detected in the Philippines. Study design: From May 2008 to April 2012, nasopharyngeal swabs were collected from infants and children aged between 7 days and 14 years who were hospitalized with severe pneumonia. HRSV was detected by nested PCR targeting M2 gene, and C-terminus of the G gene was sequenced for phylogenetic analysis. Result: Out of total 2150 samples, 19.3% (n= 415) were positive for HRSV, and 65.0% of them (n= 270) were identified as HRSV-A and 35.0% (n= 145) as HRSV-B. There were two major HRSV outbreaks: between June 2008 and February 2009, and between June and March 2012. Majority of HRSV strains detected during the former outbreak were HRSV-A (97.5%, 203/208) whereas during the later outbreak, both HRSV-A (54/158, 34.2%) and HRSV-B (104/158, 65.8%) were detected. All HRSV-A strains were classified as genotype NA1 and all HRSV-B as genotype BA, which had 60-nucleotide duplication in secondary hypervariable region of the G gene. Among HRSV-B positive samples, there were 2 distinct clusters with unique amino acid changes and low homology in compared to other strains in BA, suggesting emergence of new variant of HRSV-B. Conclusion: The study provides an overview of the genetic variation in circulating HRSV viruses in the Philippines along with identification of possibly a novel variant of HRSV-B. © 2013 Elsevier B.V.

    DOI: 10.1016/j.jcv.2013.01.001

    Scopus

    PubMed

    researchmap

▼display all

MISC

  • 古細菌ゲノムのSpike-inを利用した定量的16Sメタゲノム解析法の評価(Evaluation of quantitative 16S metagenomic analysis using spike-in archaeal genome)

    大野 歩, 高橋 麻乃, 羽原 拓哉, Kryukov Kirill, 中川 草, 今西 規

    日本細菌学雑誌   76 ( 1 )   67 - 67   2021.2

     More details

    Language:English   Publisher:日本細菌学会  

    researchmap

  • Propidium monoazideとnanopore DNAシークエンサーを用いた薬剤耐性細菌の迅速なプロファイリング法(Rapid profiling of drug-resistant bacteria using propidium monoazide and a nanopore DNA sequencer)

    大野 歩, 梅澤 和夫, 浅井 さとみ, Kryukov Kirill, 中川 草, 宮地 勇人, 今西 規

    日本細菌学雑誌   75 ( 1 )   69 - 69   2020.1

     More details

    Language:English   Publisher:日本細菌学会  

    researchmap

  • Rapid bacterial identification method using nanopore DNA sequencer

    大野歩, 中川草, KRYUKOV Kirill, KRYUKOV Kirill, 今西規

    臨床化学   49 ( 4 )   265 - 270   2020

     More details

    Language:Japanese  

    Scopus

    J-GLOBAL

    researchmap

  • MinIONを用いたDNA型判定の試み(その3)

    麻生川稔, 麻生川稔, 大野歩, 中川草, 落合恵理子, 大澤資樹, 杉澤正俊, 今西規

    日本法科学技術学会誌   25 ( Supplement )   2020

  • Nanopore DNA Sequencerを用いた迅速なウイルス検出法の検討

    大野歩, KRYUKOV Kirill, 中川草, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020

  • ナノポアDNAシークエンサーを用いた薬剤耐性菌同定技術

    大野歩, 梅澤和夫, KRYUKOV Kirill, 中川草, 浅井さとみ, 宮地勇人, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

  • MinIONを用いたDNA型判定の試み(その2)

    麻生川稔, 麻生川稔, 大野歩, 中川草, 落合恵理子, 片平泰弘, 須藤恵美, 大澤資樹, 杉澤正俊, 今西規

    日本法科学技術学会誌   24 ( Supplement )   2019

  • Propidium monoazideを用いたゲノム解析による迅速な薬剤耐性菌の同定

    大野歩, 梅澤和夫, KRYUKOV Kirill, 中川草, 浅井さとみ, 宮地勇人, 今西規

    日本ゲノム微生物学会年会要旨集   13th   2019

  • ホルスタイン種における牛白血病抵抗性・感受性を規定するウシ主要組織適合遺伝子複合体クラスIIアリルの同定

    竹嶋伸之輔, 大野歩, 間陽子

    日本獣医師会獣医学術学会年次大会講演要旨集   2017   2018

  • MinIONを用いたDNA型判定の試み(その1)

    麻生川稔, 麻生川稔, 杉澤正俊, 大野歩, 中川草, 今西規

    日本法科学技術学会誌   23 ( Supplement )   2018

  • 生菌抽出法による薬剤耐性菌の迅速なプロファイリング技術の開発

    大野歩, 梅澤和夫, KRYUKOV Kirill, 中川草, 浅井さとみ, 宮地勇人, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   41st   2018

  • 次世代シークエンサーを用いたヒト膣内細菌叢の多様性解析

    須藤恵美, 大野歩, KRYUKOV Kirill, 宮澤麻里子, 信田政子, 三上幹男, 今西規

    日本分子生物学会年会プログラム・要旨集(Web)   41st   2018

  • 全国調査に基づく乳牛および肉牛の牛白血病プロウイルス量の制御に関わる主要組織適合抗原クラスII遺伝子の検出

    竹嶋伸之輔, 大野歩, 菊谷真理, 松本有生, 小原潤子, 間陽子

    日本獣医学会学術集会講演要旨集   159th   392 - 392   2016

     More details

    Language:Japanese   Publisher:(公社)日本獣医学会  

    J-GLOBAL

    researchmap

  • Epidemiological features of BLV infection in Japan from 2012 to 2013

    Ayumu Ohno, Shin-nosuke Takeshima, Mari Kikuya, Yuki Matsumoto, Yoko Aida

    RETROVIROLOGY   12   2015.8

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:BIOMED CENTRAL LTD  

    Web of Science

    researchmap

  • Epitope mapping of CD8+T cells on bovine leukemia virus Gag, Env and Tax protein in cattle with different bovine MHC DRB3 alleles

    Lanlan Bai, Shin-nosuke Takeshima, Ayumu Ohno, Yuki Matsumoto, Emiko Isogai, Junko Kohara, Yoko Aida

    RETROVIROLOGY   12   2015.8

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:BIOMED CENTRAL LTD  

    Web of Science

    researchmap

  • 国内の牛白血病ウイルス感染牛において,BLV-CoCoMo-qPCRにより検出されたプロウイルス量の解析

    大野歩, 竹嶋伸之輔, 間陽子

    日本獣医学会学術集会講演要旨集   158th   335 - 335   2015

     More details

    Language:Japanese   Publisher:(公社)日本獣医学会  

    J-GLOBAL

    researchmap

  • BLV-CoCoMo Direct PCR法を用いた全血からの牛白血病ウイルスの検出法の開発と実証試験

    綿貫園子, 竹嶋伸之輔, 大野歩, 若松敏枝, 的場和弘, 石崎宏, 間陽子

    日本獣医学会学術集会講演要旨集   158th   335 - 335   2015

     More details

    Language:Japanese   Publisher:(公社)日本獣医学会  

    J-GLOBAL

    researchmap

  • フィリピンの牛白血病ウイルスの検出と分子性状解析(Detection and molecular characterization of bovine leukemia virus from the Philippines)

    Polat Meripet, 大野 歩, 竹嶋 伸之輔, 菊谷 真理, 松本 有生, Mingala Claro N., 小沼 操, 間 陽子

    日本獣医学会学術集会講演要旨集   157回   407 - 407   2014.8

     More details

    Language:English   Publisher:(公社)日本獣医学会  

    researchmap

  • 牛白血病ウイルスのCD8+T細胞エピトープのマッピング-異なる主要組織適合遺伝子複合体(MHC)を有するウシを用いた解析-

    BAI Lanlan, BAI Lanlan, 竹嶋伸之輔, 大野歩, 松本有生, 磯貝恵美子, 小原潤子, 間陽子

    日本ウイルス学会学術集会プログラム・抄録集   62nd   2014

  • 国内の牛白血病ウイルス感染牛において,BLV-CoCoMo-qPCRにより検出されたプロウイルス量の解析

    大野歩, 竹嶋伸之輔, 菊谷真理, 松本有生, 間陽子

    日本ウイルス学会学術集会プログラム・抄録集   62nd   2014

  • BLV-CoCoMo-qPCRを用いた国内の牛白血病ウイルス感染牛のプロウイルス量の解析

    大野歩, 竹嶋伸之輔, 菊谷真理, 松本有生, 間陽子

    日本獣医学会学術集会講演要旨集   157th   424 - 424   2014

     More details

    Language:Japanese   Publisher:(公社)日本獣医学会  

    J-GLOBAL

    researchmap

  • 異なるBoLA-DRB3アリル遺伝子を保有するウシにおける牛白血病ウイルスCD8+ T細胞エピトープのマッピング

    BAI Lanlan, BAI Lanlan, 竹嶋伸之輔, 大野歩, 松本有生, 磯貝恵美子, 小原潤子, 間陽子

    日本獣医学会学術集会講演要旨集   157th   408 - 408   2014

     More details

    Language:Japanese   Publisher:(公社)日本獣医学会  

    J-GLOBAL

    researchmap

  • 日本におけるBLV-CoCoMo-qPCRを用いた牛白血病感染牛のプロウイルス量の調査

    大野歩, 竹嶋伸之輔, 松本有生, 間陽子

    日本ウイルス学会学術集会プログラム・抄録集   61st   2013

  • Etiology of childhood pneumonia in Tacloban, the Philippines

    A. Suzuki, S. Lupisan, N. Fuji, A. Ohno, Y. Furuse, R. Tamaki, M. Saito, H. Oreste, M. Mondoy, L. Sombrero, A. De Leon, R. Olveda, H. Oshitani

    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES   14   E27 - E27   2010.3

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER SCI LTD  

    DOI: 10.1016/j.ijid.2010.02.1547

    Web of Science

    researchmap

  • 2009/2010シーズンに仙台市内で流行したRSウイルスの解析

    大野歩, 鈴木陽, 押谷仁, 川村和久

    外来小児科   13 ( 3 )   381 - 381   2010

     More details

    Language:Japanese   Publisher:(一社)日本外来小児科学会  

    J-GLOBAL

    researchmap

  • フィリピン・レイテ島における小児重症肺炎の疫学研究

    鈴木陽, 大野歩, 藤直子, 古瀬祐気

    日本小児科学会雑誌   114 ( 2 )   217 - 217   2010

     More details

    Language:Japanese   Publisher:(公社)日本小児科学会  

    J-GLOBAL

    researchmap

  • 2008~2009年フィリピン・レイテ島タクロバンにおいて小児重症肺炎患者から検出されたRSウイルス

    大野歩, 鈴木陽, 藤直子, 古瀬祐気, 玉記雷太, 齋藤麻理子, 押谷仁

    感染症学雑誌   84 ( 5 )   290 - 290   2010

     More details

    Language:Japanese   Publisher:(一社)日本感染症学会  

    J-GLOBAL

    researchmap

  • フィリピン・レイテ島における小児重症肺炎の疫学調査

    鈴木陽, 古瀬祐気, 藤直子, 大野歩, 玉記雷太, 齋藤麻理子, 押谷仁

    日本小児科学会雑誌   113 ( 12 )   1876 - 1876   2009

     More details

    Language:Japanese   Publisher:(公社)日本小児科学会  

    J-GLOBAL

    researchmap

  • ヒト下垂体・腺腫におけるNOTCHシグナル分子の発現解析

    江頭登, 大野歩, 竹井麻生, 竹腰進, 寺本明, 長村義之

    日本内分泌学会雑誌   83 ( 2 )   402 - 402   2007

  • ヒト下垂体腺腫におけるNotchシグナル伝達の解析

    江頭登, 大野歩, 竹井麻生, 梶谷華子, 宮腰隆史, 竹腰進, 寺本明, 長村義之

    日本病理学会会誌   96 ( 1 )   299 - 299   2007

     More details

    Language:Japanese   Publisher:(一社)日本病理学会  

    J-GLOBAL

    researchmap

  • 下垂体腺腫の機能分化におけるNotchシグナル伝達の解析

    江頭登, 大野歩, 竹井麻生, 梶谷華子, 宮腰隆史, 竹腰進, 寺本明, 長村義之

    日本内分泌学会雑誌   83 ( 1 )   173 - 173   2007

     More details

    Language:Japanese   Publisher:(一社)日本内分泌学会  

    J-GLOBAL

    researchmap

▼display all

Research Projects

  • 次世代シークエンサーを用いた鼻腔内混合感染の実態調査および重症化リスクの検証

    Grant number:20K17196  2020.04 - 2022.03

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    大野 歩

      More details

    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    researchmap