研究者詳細 - 坂本　亘

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Putative phospholipid hydroperoxide glutathione peroxidase gene from Arabidopsis thaliana induced by oxidative stress

Sugimoto Manabu, Sakamoto Wataru

Genes & genetic systems 72 ( 5 ) 311 - 316 1997年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：The Genetics Society of Japan

An Arabidopsis cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA comprised 803 bp and included an open reading frame which encodes a polypeptide of 169 amino acid residues. The deduced amino acid sequence showed about 80 and 50% homology with plant putative PHGPXs and mammalian PHGPXs, respectively. Southern blot analysis suggested that the putative PHGPX gene was a single-copy gene. The expression profile of the putative PHGPX in Arabidopsis under NaCl and Al/Fe treatments, which generate oxidative stress, was analyzed. Northern blot analysis revealed that the Arabidopsis putative PHGPX mRNA levels were increased about 3 and 4.5 times after exposure to NaCl and Al/Fe, respectively. These results suggest that the putative PHGPX gene is induced by oxidative stress in Arabidopsis.<br>

DOI： 10.1266/ggs.72.311

その他リンク： https://jlc.jst.go.jp/DN/JALC/00084483256?from=CiNii

Altered mitochondrial gene expression in a maternal distorted leaf mutant of Arabidopsis induced by chloroplast mutator

Sakamoto, W, Kondo, H, Murata, M, Motoyoshi, F

Plant Cell 8 ( 8 ) 1996年

Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Utsugi, S, Sakamoto, W, Ogura, Y, Murata, M, Motoyoshi, F

Plant Molecular Biology 32 ( 4 ) 1996年

PLANT-MITOCHONDRIA - FROM GENE TO FUNCTION

SAKAMOTO, W

Breeding Science 45 ( 3 ) 407 - 407 1995年

petD mRNA maturation in Chlamydomonas reinhardtii chloroplasts:role of 5′endonucleolytic processing.

SAKAMOTO W.

Mol.Cell.Biol. 14 ( 9 ) 6180 - 6186 1994年

THE PETD GENE IS TRANSCRIBED BY FUNCTIONALLY REDUNDANT PROMOTERS IN CHLAMYDOMONAS-REINHARDTII CHLOROPLASTS

STURM, NR, KURAS, R, BUSCHLEN, S, SAKAMOTO, W, KINDLE, KL, STERN, DB, WOLLMAN, FA

Molecular and Cellular Biology 14 ( 9 ) 1994年

Function of the Chlamydomonas reinhardtii petD 5'untraslated region in regulating the accumulation of subunit IV of the cytochrome b_6/f complex

SAKAMOTO W.

Plant J. 6 ( 4 ) 503 - 512 1994年

RESTRICTION FRAGMENTS HOMOLOGOUS TO MITOCHONDRIAL PLASMID-LIKE DNAS ARE LOCATED WITHIN LIMITED CHROMOSOMAL REGIONS ON THE RICE NUCLEAR GENOME

KANAZAWA, A, KISHIMOTO, N, SAKAMOTO, W, OHSAWA, R, UKAI, Y, TSUTSUMI, N, HIRAI, A, SAITO, A

Theoretical and Applied Genetics 87 ( 5 ) 1993年

In vivo analysis of Chlamydomonas chloroplast petD gene expression using stable transformation of β-glucuronidase translational fusions

SAKAMOTO W.

Proc. Natl. Acad. Sci. USA 90 ( 2 ) 497 - 501 1993年

ミトコンドリアに存在するプラスミド様DNAの栽培イネにおける分布と量的変異〔英文〕

金沢章, SAKAMOTO Wataru, NAKAGAHRA Masahiro, KADOWAKI Koh-ichi, TSUTUSMI Nobuhiro, TANO Shigemitsu

The Japanese journal of genetics 67 ( 4 ) 309 - 319 1992年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：The Genetics Society of Japan

Distribution of four kinds of mitochondrial plasmid-like DNAs, Bl, B2, B3 and B4 was investigated by Southern analysis using 85 accessions of cultivated rice (Oryza sativa L.). The frequencies of these molecules found in cultivars differed from each other. The mitochondrial genome were categorized into nine types according to their presence or absence. They were found mostly in ecospecies Indica, a few in Javanica, and not found in Japonica. The result indicated the polymorphic and monomorphic patterns of the mitochondrial genomic organization within Indica and Japonica cultivars, respectively. Quantitative variation was found among four kinds of mitochondiral plasmid-like DNAs, suggesting that these molecules were unequally distributed or replicated during the process of mitochondrial division.<br>

DOI： 10.1266/jjg.67.309

その他リンク： https://jlc.jst.go.jp/DN/JALC/00079223454?from=CiNii

RFLP analysis of nuclear DNAs homologous with mitochondrial plasmid-like DNAs in cultivated rice.

SAKAMOTO W.

Theor. Appl. Genet. 82 ( 2 ) 179 - 184 1991年

イネミトコンドリアに存在するプラスミド様DNAの核内相同部位に関する連鎖分析〔英文〕

金沢章, SAKAMOTO Wataru, KISHIMOTO Naoki, YANO Masahiro, TSUTSUMI Nobuhiro, SAITO Akira, TANO Shigemitsu

遺伝学雑誌 66 ( 5 ) 597 - 607 1991年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Genetics Society of Japan

A genetical study on the nucleotide sequences of the nuclear DNAs which share homology with rice mitochondrial plasmid-like DNAs, B1, B2, B3 and B4 was carried out. Restriction fragments of the nuclear DNAs hybridized with these plasmid-like DNAs showed polymorphisms in their length between Indica and Japonica rice cultivars. The hybridized signals found specifically in Indica or Japonica cultivars segregated in the F₂ population derived from a cross between these two subspecies. The observed ratio of the nuclear homologues in the F₂ population demonstrated that they were transmitted according to the Mendelian inheritance. The co-segregation of homologues was examined and the linkage was detected between the B1-nuclear homologue of Japonica and the B4-nuclear homologue of Indica, and also between the nuclear homologues of B2 and B3 of Indica. The linkage between the B1-nuclear homologue of Japonica and the B4-nuclear homologue of Indica was conserved in the different rice cultivars.<br>

DOI： 10.1266/jjg.66.597

その他リンク： http://agriknowledge.affrc.go.jp/RN/2010472559

GENETIC-ANALYSIS OF NUCLEAR DNAS HOMOLOGOUS TO SMALL MITOCHONDRIAL PLASMID-LIKE DNAS IN CULTIVATED RICE 査読

W SAKAMOTO, KI KADOWAKI, N KISHIMOTO, M YANO, A SAITO, S TANO

RICE GENETICS II 365 - 372 1991年

Analysis of mitochondrial DNAs from Oryza glaberrima and its cytoplasmic substituted line for Oryza sativa associated with cytoplasmic male sterility.

SAKAMOTO Wataru, KADOWAKI Koh-ichi, TANO Shigemitsu, YABUNO Tomosaburo

遺伝学雑誌 65 ( 1 ) 1 - 6 1990年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：The Genetics Society of Japan

It was described previously that the combination of the cytoplasm of a Japanese variety of Oryza sativa and the nucleus of O. glaberrima produced by successive crossings led to male sterility. In the present study, the mitochondrial DNAs (mtDNAs) were isolated from this cytoplasmic male-sterile (CMS) strain and its maintainer strain, and were analyzed with restriction enzymes and by agarose gel electrophoresis. Differences in the restriction pattern were observed between the CMS strain having sativa cytoplasm and its maintainer strain having glaberrima cytoplasm. The plasmid-like DNAs (B1 and B2), which are often observed in mitochondria of CMS strains derived from O. sativa, could not be detected by Southern hybridization analysis as well as gel electrophoresis. It was suggested that the plasmid-like DNAs were not associated with the cytoplasmic male sterility. However, the homologous sequences to B1 and B2 were detected in the nuclear genomes of all species tested.<br>

DOI： 10.1266/jjg.65.1

Web of Science

CiNii Article

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NUCLEOTIDE-SEQUENCE OF THE CYTOCHROME-OXIDASE SUBUNIT-I GENE FROM RICE MITOCHONDRIA

K KADOWAKI, T SUZUKI, S KAZAMA, T OHFUCHI, W SAKAMOTO

NUCLEIC ACIDS RESEARCH 17 ( 18 ) 7519 - 7519 1989年9月

ANALYSIS OF HOMOLOGY OF SMALL PLASMID-LIKE MITOCHONDRIAL DNAS IN THE DIFFERENT CYTOPLASMIC MALE STERILE STRAINS IN RICE

SAKAMOTO, W, MOMOSE, M, TSUTSUMI, N, TANO, S, YAMAGUCHI, H

Japanese Journal of Genetics 64 ( 1 ) 1989年

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