研究者詳細 - 坂本　亘

論文 - 坂本　亘

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Leaf-variegated mutations and their responsible genes in Arabidopsis thaliana

Sakamoto, W

Genes & Genetic Systems 78 ( 1 ) 1 - 9 2003年

記述言語：英語掲載種別：研究論文（学術雑誌）

Leaf variegation has long been known as a recessive genetic trait in higher plants. Unlike albino mutants, leaf-variegated mutants are non-lethal and thus enable us to study a novel mechanism of plastid development and maintenance. Variegation results from a defect that makes chloroplast development unstable, since at least part of the tissues gives rise to normal chloroplasts. Despite the fact that leaf-variegated mutants have contributed to the findings of maternal inheritance or have been used as genetic markers, these mutations and the responsible loci have been poorly understood at the molecular level. A comprehensive study of the leaf-variegated mutants is possible in Arabidopsis, since such mutants have been known and the cloning can be at relative ease as a model plant. Here I summarize recent progress on characterization of the Arabidopsis leaf-variegated mutants. Detailed analysis of the responsible loci revealed that variegation is caused by a defect in various metabolic pathways related to organelle functions. Thus, studies on these genes provide us with novel redundant mechanisms by which heteroplasmic organelles such as plastids and mitochondria can survive from an environmental stress.

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[Leaf-variegated mutants in higher plants as tools for studying a novel function in plastids and mitochondria].

Wataru Sakamoto

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 47 ( 12 Suppl ) 1771 - 6 2002年9月

Reduction in amounts of mitochondrial DNA in the sperm cells as a mechanism for maternal inheritance in Hordeum vulgare

Sodmergen, Zhang, QA, Zhang, YT, Sakamoto, W, Kuroiwa, T

Planta 216 ( 2 ) 2002年

The VAR1 locus of Arabidopsis encodes a chloroplastic FtsH and is responsible for leaf variegation in the mutant alleles 国際誌

Sakamoto, W, Tamura, T, Hanba-Tomita, Y, Murata, M

Genes To Cells 7 ( 8 ) 769 - 80 2002年

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: A leaf-variegated mutation var1 of Arabidopsis results in the development of abnormal plastids and the formation of a green/white sector. Genetic analysis of the var1 mutant indicated that it acts synergistically with another mutation var2, suggesting that the two genes are relevant. The VAR2 locus has been shown to encode a chloroplastic FtsH, an ATP-dependent protease which is possibly involved in the degradation of thylakoid proteins and plastid development. RESULTS: In this study we show that the VAR1 locus encodes a chloroplastic FtsH protein homologous to VAR2. VAR1 contains a conserved motif for ATPase and a metalloprotease characteristic to FtsH proteins, and is targeted into chloroplasts. A VAR1-fusion protein synthesized in vitro exhibited ATPase activity and partial metalloprotease activity. The maximum yield of photochemistry, measured by chlorophyll fluorescence, showed that the var1 mutants were sensitive to photoinhibitory light exposure at 800 micro mol/m2/s. CONCLUSION: VAR1 and VAR2 comprise an FtsH small gene family together with other FtsH genes in Arabidopsis. VAR1 as well as VAR2 may play an important role in degrading photodamaged subunits in photosystem II. Loss of VAR1 and VAR2 perhaps impairs the photoprotection mechanism and thylakoid development, causing leaf variegation as a consequence.

DOI： 10.1046/j.1365-2443.2002.00558.x

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Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel homologue

Mimida, N, Goto, K, Kobayashi, Y, Araki, T, Ahn, JH, Weigel, D, Murata, M, Motoyoshi, F, Sakamoto, W

Genes To Cells 6 ( 4 ) 2001年

The Purple leaf (Pl) locus of rice: the Pl(w) allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis

Sakamoto, W, Ohmori, T, Kageyama, K, Miyazaki, C, Saito, A, Murata, M, Noda, K, Maekawa, M

Plant and Cell Physiology 42 ( 9 ) 2001年

Mitochondrial localization of AtOXA1, an Arabidopsis homologue of yeast Oxa1p involved in the insertion and assembly of protein complexes in mitochondrial inner membrane

Sakamoto, W, Spielewoy, N, Bonnard, G, Murata, M, Wintz, H

Plant and Cell Physiology 41 ( 10 ) 2000年

The YELLOW VARIEGATED (VAR2) locus encodes a homologue of FtsH, an ATP-dependent protease in Arabidopsis

Takechi, K, Sodmergen, Murata, M, Motoyoshi, F, Sakamoto, W

Plant and Cell Physiology 41 ( 12 ) 2000年

イネ完熟種子糊粉層におけるアントシアニン生合成に係わる転写制御遺伝子のトランジェントアッセイ系

前川雅彦, 景山圭祐, 力石和英, 宇都木繁子, 野田和彦, 坂本亘

育種学研究 2 ( 4 ) 181 - 187 2000年

記述言語：日本語出版者・発行元：日本育種学会

イネにおけるアントシアニン生合成の転写制御遺伝子の発現解析に必要なトランジェントアッセイ系を, 常時材料を準備できるイネ完熟種子糊粉層で確立した. まず, 岡大資生研紫米 (RIB-PI) の完熟種子を用いて糊粉層を露出するための吸水時間と温度を検討した. 露出できた糊粉層にトウモロコシのアントシアニン生合成の転写制御遺伝子, ClとB-Peruをパーティクルガンで導入したところ, アントシアニンのスポットが観察されたので, 両遺伝子を用いたトランジェントアッセイ系について至適条件を検討した. その結果, 28℃ で4時間吸水させて露出した糊粉層でアントシアニンのスポット数が最大になった. また, 導入するプラスミドの量は, ClとBPeruがともに5μgかClが5μgでB-Peruが10μgのときに, アントシアニンのスポット数が多くなった. 以上の結果から, イネの完熟種子糊粉層を用いたトランジェントアッセイとして, 28℃ で4時間吸水させて糊粉層を露出させ, C1とB-Pmをともに5μgずつ導入することによって, 安定的にアントシアニンを誘導できることが明らかとなった.

DOI： 10.1270/jsbbr.2.181

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The strange evolutionary history of plant mitochondrial tRNAs and their aminoacyl-tRNA synthetases

Small, I, Akashi, K, Chapron, A, Dietrich, A, Duchene, AM, Lancelin, D, Marechal-Drouard, L, Menand, B, Mireau, H, Moudden, Y, Ovesna, J, Peeters, N, Sakamoto, W, Souciet, G, Wintz, H

Journal of Heredity 90 ( 3 ) 1999年

In situ RNA hybridization using Technovit resin in Arabidopsis thaliana

Takechi, K, Sakamoto, W, Katsuhara, M, Murata, M, Motoyoshi, F

Plant Molecular Biology Reporter 17 ( 1 ) 1999年

TERMINAL FLOWER 1-like genes in Brassica species

Mimida, N, Sakamoto, W, Murata, M, Motoyoshi, F

Plant Science 142 ( 2 ) 1999年

Characterization of a flower-specific gene encoding a putative myrosinase binding protein in Arabidopsis thaliana

Takechi, K, Sakamoto, W, Utsugi, S, Murata, M, Motoyoshi, F

Plant and Cell Physiology 40 ( 12 ) 1999年

A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana

Menand, B, Marechal-Drouard, L, Sakamoto, W, Dietrich, A, Wintz, H

Proceedings of the National Academy of Sciences of the United States of America 95 ( 18 ) 1998年

Mix 'n' match - Plant mitochondrial tRNAS and their aminoacyl-tRNA synthetases

Small, I, Akashi, K, Chapron, A, Dietrich, A, Duchene, AM, Lancelin, D, Marechal-Drouard, L, Menand, B, Mireau, H, Moudden, Y, Ovesna, J, Peeters, N, Sakamoto, W, Souciet, G, Wintz, H, Moller, IM, Gardestrom, P, Glimelius, K, Glaser, E

Plant Mitochondria: From Gene To Function 1998年

Arabidopsis thaliana vegetative storage protein (VSP) genes: gene organization and tissue-specific expression

Utsugi, S, Sakamoto, W, Murata, M, Motoyoshi, F

Plant Molecular Biology 38 ( 4 ) 1998年

Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity

Cardazzo, B, Hamel, P, Sakamoto, W, Wintz, H, Dujardin, G

Gene 221 ( 1 ) 1998年

Cloning and molecular analysis of the Arabidopsis gene Terminal Flower 1

Ohshima, S, Murata, M, Sakamoto, W, Ogura, Y, Motoyoshi, F

Molecular & General Genetics 254 ( 2 ) 1997年

AN UNUSUAL ATP9-RPL16 COTRANSCRIPTIN THE MATERNAL DISTORTED LEAF MUTANT OF ARABIDOPSIS THALIANA

SAKAMOTO Wataru, MURATA Minoru, MOTOYOSHI Fusao

Plant and cell physiology 38 ( 8 ) s55 1997年

Functional complementation of an oxa1(-) yeast mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes

Hamel, P, Sakamoto, W, Wintz, H, Dujardin, G

Plant Journal 12 ( 6 ) 1997年

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