Faculty Profiles - FUJII Nobuharu

MISC - FUJII Nobuharu

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Allogeneic peripheral blood stem cell transplantation for the treatment of chronic active Epstein-Barr virus infection

N Fujii, K Takenaka, A Hiraki, Y Maeda, K Ikeda, K Shinagawa, A Ashiba, M Munemasa, K Sunami, Y Hiramatsu, F Ishimaru, K Niiya, T Yoshino, M Harada

BONE MARROW TRANSPLANTATION 26 ( 7 ) 805 - 808 2000.10

Language：English Publisher：NATURE PUBLISHING GROUP

The prognosis of chronic active Epstein-Barr virus infection (CAEBV) is very poor. We describe a 24-year-old male with severe CAEBV who was treated with allogeneic peripheral blood stem cell transplantation (allo-PBSCT), On admission, EBER-1 in lymphocytes infiltrating the liver, EBV-DNA in peripheral blood mononuclear cells (PBMC) and monoclonal NK cell proliferation were confirmed. After unsuccessful chemotherapy, he received an allo-PBSCT from his HLA-identical sister. Although he died of pulmonary hemorrhage on day +19, EBV-DNA was undetectable by PCR in PBMC, and the post-mortem liver showed no EBER-1-positive lymphocytes. This experience suggests that EBV-positive lymphocytes in CAEBV may be eradicated by allo-PBSCT, thereby raising the possibility of a new treatment modality.

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Allogeneic peripheral blood stem cell transplantation for the treatment of chronic active Epstein-Barr virus infection

N Fujii, K Takenaka, A Hiraki, Y Maeda, K Ikeda, K Shinagawa, A Ashiba, M Munemasa, K Sunami, Y Hiramatsu, F Ishimaru, K Niiya, T Yoshino, M Harada

BONE MARROW TRANSPLANTATION 26 ( 7 ) 805 - 808 2000.10

Language：English Publisher：NATURE PUBLISHING GROUP

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Allogeneic Peripheral Blood Stem Cell Transplantation in 23 Adult Patients With Hematologic Malignancies: A Single-Center Experience

TAKENAKA Katsuto, SHINAGAWA Katsuji, SUNAMI Kazutaka, FUJII Nobuharu, HIRAMATSU Yasushi, MAEDA Yoshinobu, NAWA Yuichiro, KATAYAMA Yoshio, TESHIMA Takanori, ISHIMARU Fumihiko, KIURA Katsuyuki, IKEDA Kazuma, HARADA Mine

Int J Hematol 72 ( 3 ) 362 - 370 2000.10

A CASE OF ANAPHYLAXIS AFTER PLATELET CONCENTRATE TRANSFUSION WITH ANTI-IGAM (1) AND C9 ANTIBODIES

46 ( 3 ) 324 - 329 2000

Successful engraftment of allogeneic peripheral blood stem cell transplant after nonmyeloablative preparative regimen

Int J Hematol 72 ( 4 ) 499 2000

Decreases in Ikaros activity correlate with blast crisis in patients with chronic myelogenous leukemia

H Nakayama, F Ishimaru, N Avitahl, N Sezaki, N Fujii, K Nakase, Y Ninomiya, A Harashima, J Minowada, J Tsuchiyama, K Imajoh, T Tsubota, S Fukuda, T Sezaki, K Kojima, M Hara, H Takimoto, S Yorimitsu, Takahashi, I, A Miyata, S Taniguchi, Y Tokunaga, H Gondo, Y Niho, S Nakao, T Kyo, H Dohy, N Kamada, M Harada

CANCER RESEARCH 59 ( 16 ) 3931 - 3934 1999.8

Language：English Publisher：AMER ASSOC CANCER RESEARCH

Gene targeting studies in mice have shown that the lack of Ikaros activity leads to T-cell hyperproliferation and T-cell neoplasia, establishing the Ikaros gene as a tumor suppressor gene in mice. This prompted us to investigate whether mutations in Ikaros play a role in human hematological malignancies. Reverse transcription-PCR was used to determine the relative expression Levels of Ikaros isoforms in a panel of human leukemia/lymphoma cell lines and human bone marrow samples from patients with hematological malignancies. Among the cell lines examined, only BV-173, which was derived from a chronic myelogenous Leukemia (CML) patient in lymphoid blast crisis, overexpressed the dominant-negative isoform, Ik-6. In 9 of 17 samples of patients in blast crisis of CML, Ikaros activity had been reduced either by drastically reducing mRNA expression (4 of 17) or by overexpressing the dominant-negative isoform Ik-6 (5 of 17). Significantly, expression of Ikaros isoforms seemed normal in chronic phase CML patients and patients with other hematological malignancies. In some cases, overexpression of the dominant-negative Ik-6 protein was confirmed by Western blot analysis, and Southern blot analysis indicated that decreases in Ikaros activity correlated with a mutation in the Ikaros Locus. In summary, these findings suggest that a reduction of Ikaros activity may be an important step in the development of blast crisis in CML and provide further evidence that mutations that alter Ikaros expression may contribute to human hematological malignancies.

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Molecular characterization of total kininogen deficiency in Japanese patients

F Ishimaru, H Dansako, K Nakase, N Fujii, N Sezaki, H Nakayama, N Fujii, Y Komiyama, K Iijima, K Takenaka, T Teshima, K Shinagawa, K Ikeda, K Niiya, M Harada

INTERNATIONAL JOURNAL OF HEMATOLOGY 69 ( 2 ) 126 - 128 1999.2

Language：English Publisher：CARDEN JENNINGS PUBL CO LTD

Kininogens are multifunctional plasma glycoproteins. There are two forms of human kininogen: low molecular weight kininogen (LK) and high molecular weight kininogen (HK). Both are derived from the same gene by alternative splicing. Same patients with kininogen deficiency have been reported to be deficient only in HK while others are deficient in both HK and LK (total kininogen deficiency). We analyzed three Japanese patients with total kininogen deficiency by the Csp45I digestion study of exon 5 as previously reported in Williams trait and found that two had the same point mutation of C to T at base 22 of exon 5, resulting in a transition of CGA (Arg) codon to TGA (Stop) codon. This is the first report of molecular characterization of total kininogen deficiency in the Japanese population. (C) 1999 The Japanese Society of Hematology.

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