Updated on 2024/02/02

写真a

 
KONISHI Noriyuki
 
Organization
Institute of Plant Science and Resources Assistant Professor
Position
Assistant Professor
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Degree

  • 博士 (農学) ( 2017.3   東北大学 )

Research Areas

  • Life Science / Plant nutrition and soil science

  • Environmental Science/Agriculture Science / Environmental agriculture  / 植物栄養学

Education

  • Tohoku University   大学院農学研究科   応用生命科学専攻

    2012.4 - 2017.3

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    Country: Japan

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  • Tohoku University   農学部   応用生物化学科

    2008.4 - 2012.3

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    Country: Japan

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Research History

  • Okayama University   Institute of Plant Science and Resources   Assistant Professor

    2022.10

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  • Okayama University   Institute of Plant Science and Resources

    2022.4 - 2022.9

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  • Okayama University   Institute of Plant Science and Resources

    2019.4 - 2022.3

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  • Okayama University   Institute of Plant Science and Resources

    2017.4 - 2019.3

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Professional Memberships

 

Papers

  • Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice. Reviewed International journal

    Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Jian Feng Ma

    The New phytologist   2023.12

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    To play essential roles of manganese (Mn) in plant growth and development, it needs to be transported to different organs and tissues after uptake. However, the molecular mechanisms underlying Mn distribution between different tissues are poorly understood. We functionally characterized a member of rice natural resistance-associated macrophage protein (NRAMP) family, OsNramp5 in terms of its tissue specificity of gene expression, cell-specificity of protein localization, phenotypic analysis of leaf growth and response to Mn fluctuations. OsNramp5 is highly expressed in the leaf sheath. Immunostaining revealed that OsNramp5 is polarly localized at the proximal side of xylem parenchyma cells of the leaf sheath. Both the gene expression and protein abundance of OsNramp5 are unaffected by different Mn concentrations. Knockout of OsNramp5 decreased the distribution of Mn to the leaf sheath, but increased the distribution to the leaf blade at both low and high Mn supplies, resulting in reduced growth of leaf sheath. Furthermore, expression of OsNramp5 under the control of root-specific promoter in osnramp5 mutant complemented Mn uptake, but could not complement Mn distribution to the leaf sheath. These results indicate that OsNramp5 expressed in the leaf sheath plays an important role in unloading Mn from the xylem for the local distribution in rice.

    DOI: 10.1111/nph.19454

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  • Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues. Reviewed International journal

    Noriyuki Konishi, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    The Plant cell   35 ( 6 )   2232 - 2250   2023.5

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    Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.

    DOI: 10.1093/plcell/koad073

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  • Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice Reviewed

    Noriyuki Konishi, Sheng Huang, Naoki Yamaji, Jian Feng Ma

    Plant and Cell Physiology   2022.5

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    DOI: 10.1093/pcp/pcac032

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  • A pericycle‐localized silicon transporter for efficient xylem loading in rice Reviewed

    Sheng Huang, Naoki Yamaji, Gen Sakurai, Namiki Mitani‐Ueno, Noriyuki Konishi, Jian Feng Ma

    New Phytologist   234 ( 1 )   197 - 208   2022.4

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    DOI: 10.1111/nph.17959

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17959

  • Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway Reviewed

    Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma

    Plant Physiology   188 ( 3 )   1649 - 1664   2021.12

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    <title>Abstract</title>
    Uptake of boron (B) in rice (Oryza sativa) is mediated by the Low silicon rice 1 (OsLsi1) channel, belonging to the NOD26-like intrinsic protein III subgroup, and the efflux transporter B transporter 1 (OsBOR1). However, it is unknown how these transporters cooperate for B uptake and how they are regulated in response to B fluctuations. Here, we examined the response of these two transporters to environmental B changes at the transcriptional and posttranslational level. OsBOR1 showed polar localization at the proximal side of both the exodermis and endodermis of mature root region, forming an efficient uptake system with OsLsi1 polarly localized at the distal side of the same cell layers. Expression of OsBOR1 and OsLsi1 was unaffected by B deficiency and excess. However, although OsLsi1 protein did not respond to high B at the protein level, OsBOR1 was degraded in response to high B within hours, which was accompanied with a significant decrease of total B uptake. The high B-induced degradation of OsBOR1 was inhibited in the presence of MG-132, a proteasome inhibitor, without disturbance of the polar localization. In contrast, neither the high B-induced degradation of OsBOR1 nor its polarity was affected by induced expression of dominant-negative mutated dynamin-related protein 1A (OsDRP1AK47A) or knockout of the mu subunit (AP2M) of adaptor protein-2 complex, suggesting that clathrin-mediated endocytosis is not involved in OsBOR1 degradation and polar localization. These results indicate that, in contrast to Arabidopsis thaliana, rice has a distinct regulatory mechanism for B uptake through clathrin-independent degradation of OsBOR1 in response to high B.

    DOI: 10.1093/plphys/kiab575

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  • Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition Reviewed

    Noriyuki Konishi, Jian Feng Ma

    New Phytologist   232 ( 4 )   1778 - 1792   2021.11

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    Ammonium is a preferential nitrogen form for rice (Oryza sativa) grown in paddy field, but the molecular mechanisms for ammonium uptake have not been well understood. We functionally characterized three members belonging to ammonium transporter 1 (AMT1) and investigated their contributions to ammonium uptake. Spatial expression analysis showed that the upregulated expression of OsAMT1;1 and OsAMT1;2 and downregulated expression of OsAMT1;3 by ammonium were higher in the root mature region than in the root tips. All OsAMT1 members were polarly localized at the distal side of exodermis in the mature region of crown roots and lateral roots. Upon exposure to ammonium, localization of OsAMT1;1 and OsAMT1;2 was also observed in the endoplasmic reticulum, but their abundance in the plasma membrane was not changed. Single knockout of either gene did not affect ammonium uptake, but knockout of all three genes resulted in 95% reduction of ammonium uptake. However, the nitrogen uptake did not differ between the wild-type rice and triple mutants at high ammonium and nitrate supply. Our results indicate that three OsAMT1 members are cooperatively required for uptake of low ammonium in rice roots and that they undergo a distinct regulatory mechanism in response to ammonium.

    DOI: 10.1111/nph.17679

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17679

  • Overexpression of the manganese/cadmium transporter OsNRAMP5 reduces cadmium accumulation in rice grain Reviewed

    Jia-Dong Chang, Sheng Huang, Noriyuki Konishi, Peng Wang, Jie Chen, Xin-Yuan Huang, Jian Feng Ma, Fang-Jie Zhao

    JOURNAL OF EXPERIMENTAL BOTANY   71 ( 18 )   5705 - 5715   2020.9

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    Rice is a major dietary source of the toxic metal cadmium (Cd), and reducing its accumulation in the grain is therefore important for food safety. We selected two cultivars with contrasting Cd accumulation and generated transgenic lines overexpressing OsNRAMP5, which encodes a major influx transporter for manganese (Mn) and Cd. We used two different promoters to control the expression, namely OsActin1 and maize Ubiquitin. Overexpression of OsNRAMP5 increased Cd and Mn uptake into the roots, but markedly decreased Cd accumulation in the shoots, whilst having a relatively small effect on Mn accumulation in the shoots. The overexpressed OsNRAMP5 protein was localized to the plasma membrane of all cell types in the root tips and lateral root primordia without polarity. Synchrotron X-ray fluorescence mapping showed that the overexpression lines accumulated more Cd in the root tips and lateral root primordia compared with the wild-type. When grown in three Cd-contaminated paddy soils, overexpression of OsNRAMP5 decreased concentration of Cd in the grain by 49-94% compared with the wild type. OsNRAMP5-overexpression plants had decreased Cd translocation from roots to shoots as a result of disruption of its radial transport into the stele for xylem loading, demonstrating the effect of transporter localization and polarity on ion homeostasis.

    DOI: 10.1093/jxb/eraa287

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  • 百科繚覧 Vol 1 : 若手研究者が挑む学際フロンティア

    Noriyuki Konishi

    2019.1

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  • The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions Reviewed

    Marcel P. Beier, Takayuki Fujita, Kazuhiro Sasaki, Keiichi Kanno, Miwa Ohashi, Wataru Tamura, Noriyuki Konishi, Masahide Saito, Fumi Imagawa, Keiki Ishiyama, Akio Miyao, Tomoyuki Yamaya, Soichi Kojima

    Physiologia Plantarum   167 ( 1 )   75 - 89   2019

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    © 2018 Scandinavian Plant Physiology Society Nitrogen is one of the most important elements for plant growth, and urea is one of the most frequently used nitrogen fertilizers worldwide. Besides the exogenously-supplied urea to the soil, urea is endogenously synthesized during secondary nitrogen metabolism. Here, we investigated the contribution of a urea transporter, DUR3, to rice production using a reverse genetic approach combined with localization studies. Tos17 insertion lines for DUR3 showed a 50% yield reduction in hydroponic culture, and a 26.2% yield reduction in a paddy field, because of decreased grain filling. Because shoot biomass production and shoot total N was not reduced, insertion lines were disordered not only in nitrogen acquisition but also in nitrogen allocation. During seed development, DUR3 insertion lines accumulated nitrogen in leaves and could not sufficiently develop their panicles, although shoot and root dry weights were not significantly different from the wild-type. The urea concentration in old leaf harvested from DUR3 insertion lines was lower than that in wild-type. DUR3 promoter-dependent β-glucuronidase (GUS) activity was localized in vascular tissue and the midribs of old leaves. These results indicate that DUR3 contributes to nitrogen translocation and rice yield under nitrogen-deficient and field conditions.

    DOI: 10.1111/ppl.12872

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  • Outgrowth of Rice Tillers Requires Availability of Glutamine in the Basal Portions of Shoots Reviewed

    Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Noriyuki Konishi, Kazuhiro Sasaki, Mitsue Miyao, Toshihiko Hayakawa, Tomoyuki Yamaya

    Rice   11 ( 1 )   2018.12

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    © 2018, The Author(s). Background: Our previous studies concluded that metabolic disorder in the basal portions of rice shoots caused by a lack of cytosolic glutamine synthetase1;2 (GS1;2) resulted in a severe reduction in the outgrowth of tillers. Rice mutants lacking GS1;2 (gs1;2 mutants) showed a remarkable reduction in the contents of both glutamine and asparagine in the basal portions of shoots. In the current study, we attempted to reveal the mechanisms for this decrease in asparagine content using rice mutants lacking either GS1;2 or asparagine synthetase 1 (AS1). The contributions of the availability of glutamine and asparagine to the outgrowth of rice tillers were investigated. Results: Rice has two AS genes, and the enzymes catalyse asparagine synthesis from glutamine. In the basal portions of rice shoots, expression of OsAS1, the major species in this tissue, was reduced in gs1;2 mutants, whereas OsAS2 expression was relatively constant. OsAS1 was expressed in phloem companion cells of the nodal vascular anastomoses connected to the axillary bud vasculatures in the basal portions of wild-type shoots, whereas cell-specific expression was markedly reduced in gs1;2 mutants. OsAS1 was up-regulated significantly by NH4+ supply in the wild type but not in gs1;2 mutants. When GS reactions were inhibited by methionine sulfoximine, OsAS1 was up-regulated by glutamine but not by NH4+. The rice mutants lacking AS1 (as1 mutants) showed a decrease in asparagine content in the basal portions of shoots. However, glutamine content and tiller number were less affected by the lack of AS1. Conclusion: These results indicate that in phloem companion cells of the nodal vascular anastomoses, asparagine synthesis is largely dependent on glutamine or its related metabolite-responsive AS1. Thus, the decrease in glutamine content caused by a lack of GS1;2 is suggested to result in low expression of OsAS1, decreasing asparagine content. However, the availability of asparagine generated from AS1 reactions is apparently less effective for the outgrowth of tillers. With respect to the tiller number and the contents of glutamine and asparagine in gs1;2 and as1 mutants, the availability of glutamine rather than asparagine in basal portions of rice shoots may be required for the outgrowth of rice tillers.

    DOI: 10.1186/s12284-018-0225-2

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  • Decreasing arsenic accumulation in rice by overexpressing OsNIP1;1 and OsNIP3;3 through disrupting arsenite radial transport in roots Reviewed

    Sheng Kai Sun, Yi Chen, Jing Che, Noriyuki Konishi, Zhong Tang, Anthony J. Miller, Jian Feng Ma, Fang Jie Zhao

    New Phytologist   219 ( 2 )   641 - 653   2018.7

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    © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust Rice is a major dietary source of the toxic metalloid arsenic. Reducing arsenic accumulation in rice grain is important for food safety. We generated transgenic rice overexpressing two aquaporin genes, OsNIP1;1 and OsNIP3;3, under the control of a maize ubiquitin promoter or the rice OsLsi1 promoter, and tested the effect on arsenite uptake and translocation. OsNIP1;1 and OsNIP3;3 were highly permeable to arsenite in Xenopus oocyte assays. Both transporters were localized at the plasma membrane. Knockout of either gene had little effect on arsenite uptake or translocation. Overexpression of OsNIP1;1 or OsNIP3;3 in rice did not affect arsenite uptake but decreased root-to-shoot translocation of arsenite and shoot arsenic concentration markedly. The overexpressed OsNIP1;1 and OsNIP3;3 proteins were localized in all root cells without polarity. Expression of OsNIP1;1 driven by the OsLsi1 promoter produced similar effects. When grown in two arsenic-contaminated paddy soils, overexpressing lines contained significantly lower arsenic concentration in rice grain than the wild-type without compromising plant growth or the accumulation of essential nutrients. Overexpression of OsNIP1;1 or OsNIP3;3 provides a route for arsenite to leak out of the stele, thus restricting arsenite loading into the xylem. This strategy is effective in reducing arsenic accumulation in rice grain.

    DOI: 10.1111/nph.15190

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  • Cytosolic Glutamine Synthetase Isozymes Play Redundant Roles in Ammonium Assimilation under Low-Ammonium Conditions in Roots of Arabidopsis thaliana Reviewed

    Noriyuki Konishi, Masahide Saito, Fumi Imagawa, Keiich Kanno, Tomoyuki Yamaya, Soichi Kojima

    Plant and Cell Physiology   59 ( 3 )   601 - 613   2018.3

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    © The Author(s) 2018. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. Ammonium is a major nitrogen source for plants; it is assimilated into glutamine via a reaction catalyzed by glutamine synthetase (GLN). Arabidopsis expresses four cytosolic GLN genes, GLN1; 1, GLN1; 2, GLN1; 3 and GLN1; 4, in roots. However, the function and organization of these GLN1 isozymes in ammonium assimilation in roots remain unclear. In this study, we aimed to characterize the four GLN1 isozymes. The levels of growth of the wild type and gln1 single and multiple knockout lines were compared in a hydroponic culture at ammonium concentrations of 0.1 and 3 mM. Under the low-ammonium concentration, in single mutants for each GLN1 gene, there was little effect on growth, whereas the triple mutant for GLN1; 1, GLN1; 2 and GLN1; 3 grew slowly and accumulated ammonium. Under the high-ammonium concentration, the single mutant for GLN1; 2 showed 50% decreases in fresh weight and glutamine, whereas the other gln1 single mutants did not show notable changes in the phenotype. The double mutant for GLN1; 1 and GLN1; 2 showed less growth and a lower glutamine concentration than the single mutant for GLN1; 2. Promoter analysis indicated an overlapping expression of GLN1; 1 with GLN1; 2 in the surface layers of the roots. We thus concluded that: (i) at a low concentration, ammonium was assimilated by GLN1; 1, GLN1; 2 and GLN1; 3, and they were redundant; (ii) low-affinity GLN1; 2 could contribute to ammonium assimilation at concentrations ranging from 0.1 to 3 mM; and (iii) GLN1; 1 supported GLN1; 2 within the outer cell layers of the root.

    DOI: 10.1093/pcp/pcy014

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  • A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains Reviewed

    Yui Yabuki, Miwa Ohashi, Fumi Imagawa, Keiki Ishiyama, Marcel Pascal Beier, Noriyuki Konishi, Toshiko Umetsu-Ohashi, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima

    Rice   10 ( 1 )   1 - 10   2017.12

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    © 2017, The Author(s). Background: Asparagine is one of the most dominant organic nitrogen compounds in phloem and xylem sap in a wide range of plant species. Asparaginase (ASNase; EC, 3.5.1.1) catabolizes asparagine into aspartate and ammonium; therefore, it is suggested to play a key role in asparagine metabolism within legume sink organs. However, the metabolic fate of asparagine in source and sink organs during rice seed production remains to be elucidated. Therefore, the main objective of this study is to investigate the asparagine metabolism in a temporal and spatial manner during rice seed production. Results: For this purpose, the expression of genes involved in asparagine catabolism, such as asparaginase1 (OsASNase1) and 2 (OsASNase2), were quantitatively measured, and contents of asparagine, aspartate and ammonium ions were determined in sink and source organs during spikelet ripening. Quantitative real-time PCR and in situ localization studies determined that OsASNase2 is expressed in the dorsal vascular bundles and nucellar projection of developing grains, as well as in mesophyll and phloem companion cells of senescent flag leaves. Amino acid measurements revealed that the aspartate concentration is higher than asparagine in both source and sink organs. Conclusion: This work suggests that asparaginase dependent asparagine catabolism occurred not only in sink but also in source organs.

    DOI: 10.1186/s12284-017-0143-8

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  • Ammonium uptake capacity and response of cytosolic glutamine synthetase 1;2 to ammonium supply are key factors for the adaptation of ammonium nutrition in Arabidopsis thaliana Reviewed

    Takanori Yasuda, Noriyuki Konishi, Soichi Kojima

    Soil Science and Plant Nutrition   63 ( 6 )   553 - 560   2017.11

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    © 2017 Japanese Society of Soil Science and Plant Nutrition. Plant requires nitrogen for the growth, and it use nitrate and ammonium from the environment. Plant suffers from the toxicity when excess ammonium is supplied as a sole nitrogen, although it could be a good nitrogen source for plant growth. We hypothesized that the different responses of ecotypes to ammonium nutrient could partly account for the adaptation of Arabidopsis to an ammonium environment. The purpose of this study is to understand the different responses of ecotypes in ammonium environment. The growth of Arabidopsis thaliana ecotypes, Columbia was compared to those of Arabidopsis thaliana ecotypes, Landsberg erecta in ammonium nutrient. The ratio of shoot dry weight to root dry weight was compared to evaluate the adaptation of two ecotypes. The shoot:root ratio of Landsberg was significantly higher than that of Columbia. T-DNA insertion in cytosolic glutamine synthetase 1;2, one of the essential ammonium assimilatory enzymes, led a decrease of shoot:root ratio. We also measured the isotope-labeled ammonium uptake and the expression levels of ammonium transporter genes, and also the expression of ammonium assimilatory genes, glutamine synthetase genes and glutamate synthase genes, in roots after ammonium re-supply using real-time polymerase chain reaction analysis. We found that (1) ammonium uptake of Landsberg erecta was higher than that of Columbia, when ammonium was supplied at higher concentration, and (2) cytosolic glutamine synthetase 1;2 was highly increased by ammonium supply in the root of Landsberg erecta. The present study suggested the importance of these two factors for adaptation of Arabidopsis to an ammonium-rich environment.

    DOI: 10.1080/00380768.2017.1395292

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  • Transcriptional repressor IAA17 is involved in nitrogen use by modulating cytosolic glutamine synthetase GLN1;2 in Arabidopsis roots Reviewed

    Masahide Saito, Noriyuki Konishi, Keiichi Kanno, Tomoyuki Yamaya, Soichi Kojima

    Soil Science and Plant Nutrition   63 ( 2 )   163 - 170   2017.3

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    © 2017 Japanese Society of Soil Science and Plant Nutrition. Nitrogen is an essential element for plant growth. Development of the root system architecture is highly correlated with nitrogen availability from the environment. Recent studies show that auxin response modules are involved in nitrate-dependent lateral root growth. However, the role of auxin in nitrogen metabolism remains to be elucidated. Few researchers have addressed the effect of auxin signaling modules on the use of ammonium for nutrition. The purpose of this study was to describe and examine the relation between auxin signaling modules and ammonium for nutrition. The growth of T-DNA insertion lines for auxin signaling modules was compared with that of a wild type under different nitrogen regimes. Nitrogen use efficiency consists of two components: metabolism and uptake. The nitrogen usage index was calculated following elemental analysis to evaluate nitrogen metabolism. Isotope-labeled ammonium uptake was measured under nitrogen-deficient and -sufficient conditions. Transcriptional levels and accumulation of enzymes necessary for primary ammonium assimilation, glutamine synthetase and glutamate synthase were evaluated. One of the T-DNA insertion lines for the auxin signaling module, IAA17, showed severe growth reduction in hydroponic solution containing ammonium as a major nitrogen source. The accumulation of cytosolic glutamine synthetase was reduced in the roots of iaa17. The expression of cytosolic glutamine synthetase 1;2 in iaa17 did not respond to ammonium supply. Here we show that the auxin signaling module has an effect on ammonium use by regulating the transcriptional level of cytosolic glutamine synthetase 1;2 in the root, the gene essential for ammonium assimilation.

    DOI: 10.1080/00380768.2017.1314178

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  • Contributions of two cytosolic glutamine synthetase isozymes to ammonium assimilation in Arabidopsis roots Reviewed

    Noriyuki Konishi, Keiki Ishiyama, Marcel Pascal Beier, Eri Inoue, Keiichi Kanno, Tomoyuki Yamaya, Hideki Takahashi, Soichi Kojima

    Journal of Experimental Botany   68 ( 3 )   613 - 625   2017

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    © The Author 2017. Glutamine synthetase (GS) catalyzes a reaction that incorporates ammonium into glutamate and yields glutamine in the cytosol and chloroplasts. Although the enzymatic characteristics of the GS1 isozymes are well known, their physiological functions in ammonium assimilation and regulation in roots remain unclear. In this study we show evidence that two cytosolic GS1 isozymes (GLN1;2 and GLN1;3) contribute to ammonium assimilation in Arabidopsis roots. Arabidopsis T-DNA insertion lines for GLN1;2 and GLN1;3 (i.e. gln1;2 and gln1;3 single-mutants), the gln1;2:gln1;3 double-mutant, and the wild-type accession (Col-0) were grown in hydroponic culture with variable concentrations of ammonium to compare their growth, and their content of nitrogen, carbon, ammonium, and amino acids. GLN1;2 and GLN1;3 promoter-dependent green fluorescent protein was observed under conditions with or without ammonium supply. Loss of GLN1;2 caused significant suppression of plant growth and glutamine biosynthesis under ammonium-replete conditions. In contrast, loss of GLN1;3 caused slight defects in growth and Gln biosynthesis that were only visible based on a comparison of the gln1;2 single-and gln1;2:gln1;3 double-mutants. GLN1;2, being the most abundantly expressed GS1 isozyme, markedly increased following ammonium supply and its promoter activity was localized at the cortex and epidermis, while GLN1;3 showed only low expression at the pericycle, suggesting their different physiological contributions to ammonium assimilation in roots. The GLN1;2 promoter-deletion analysis identified regulatory sequences required for controlling ammonium-responsive gene expression of GLN1;2 in Arabidopsis roots. These results shed light on GLN1 isozyme-specific regulatory mechanisms in Arabidopsis that allow adaptation to an ammonium-replete environment.

    DOI: 10.1093/jxb/erw454

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  • Nitrate supply-dependent shifts in communities of root-associated bacteria in Arabidopsis Reviewed

    Noriyuki Konishi, Takashi Okubo, Tomoyuki Yamaya, Toshihiko Hayakawa, Kiwamu Minamisawa

    Microbes and Environments   32 ( 4 )   314 - 323   2017

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE  

    © 2017, Japanese Society of Microbial Ecology. All rights reserved. Root-associated bacterial communities are necessary for healthy plant growth. Nitrate is a signal molecule as well as a major nitrogen source for plant growth. In this study, nitrate-dependent alterations in root-associated bacterial communities and the relationship between nitrate signaling and root-associated bacteria in Arabidopsis were examined. The bacterial community was analyzed by a ribosomal RNA intergenic spacer analysis (RISA) and 16S rRNA amplicon sequencing. The Arabidopsis root-associated bacterial community shifted depending on the nitrate amount and timing of nitrate application. The relative abundance of operational taxonomic units of 25.8% was significantly changed by the amount of nitrate supplied. Moreover, at the family level, the relative abundance of several major root-associated bacteria including Burkholderiaceae, Paenibacillaceae, Bradyrhizobiaceae, and Rhizobiaceae markedly fluctuated with the application of nitrate. These results suggest that the application of nitrate strongly affects root-associated bacterial ecosystems in Arabidopsis. Bulk soil bacterial communities were also affected by the application of nitrate; however, these changes were markedly different from those in root-associated bacteria. These results also suggest that nitrate-dependent alterations in root-associated bacterial communities are mainly affected by plant-derived factors in Arabidopsis. T-DNA insertion plant lines of the genes for two transcription factors involved in nitrate signaling in Arabidopsis roots, NLP7 and TCP20, showed similar nitrate-dependent shifts in root-associated bacterial communities from the wild-type, whereas minor differences were observed in root-associated bacteria. Thus, these results indicate that NLP7 and TCP20 are not major regulators of nitrate-dependent bacterial communities in Arabidopsis roots.

    DOI: 10.1264/jsme2.ME17031

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  • Asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots Reviewed

    Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Noriyuki Konishi, Kentaro Nakano, Keiichi Kanno, Toshihiko Hayakawa, Tomoyuki Yamaya

    Plant and Cell Physiology   56 ( 4 )   769 - 778   2015

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. Asparagine is synthesized from glutamine by the reaction of asparagine synthetase (AS) and is the major nitrogen form in both xylem and phloem sap in rice (Oryza sativa L.). There are two genes encoding AS, OsAS1 and OsAS2, in rice, but the functions of individual AS isoenzymes are largely unknown. Cell type- And NH+<inf>4</inf> -inducible expression of OsAS1 as well as analyses of knockout mutants were carried out in this study to characterize AS1. OsAS1 was mainly expressed in the roots, with in situ hybridization showing that the corresponding mRNA was specifically accumulated in the three cell layers of the root surface (epidermis, exodermis and sclerenchyma) in an NH+<inf>4</inf> -dependent manner. Conversely, OsAS2 mRNA was abundant in leaf blades and sheathes of rice. Although OsAS2 mRNA was detectable in the roots, its content decreased when NH+<inf>4</inf> was supplied. Retrotransposon-mediated knockout mutants lacking AS1 showed slight stimulation of shoot length and slight reduction in root length at the seedling stage. On the other hand, the mutation caused an approximately 80-90% reduction in free asparagine content in both roots and xylem sap. These results suggest that AS1 is responsible for the synthesis of asparagine in rice roots following the supply of NH+<inf>4</inf>. Characteristics of the NH+<inf>4</inf> -dependent increase and the root surface cell-specific expression of OsAS1 gene are very similar to our previous results on cytosolic glutamine synthetase1;2 and NADH-glutamate synthase1 in rice roots. Thus, AS1 is apparently coupled with the primary assimilation of NH+<inf>4</inf> in rice roots.

    DOI: 10.1093/pcp/pcv005

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  • NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in the Arabidopsis root Reviewed

    Noriyuki Konishi, Keiki Ishiyama, Kaya Matsuoka, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima

    Physiologia Plantarum   152 ( 1 )   138 - 151   2014.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    Plant roots under nitrogen deficient conditions with access to both ammonium and nitrate ions, will take up ammonium first. This preference for ammonium rather than nitrate emphasizes the importance of ammonium assimilation machinery in roots. Glutamine synthetase (GS) and glutamate synthase (GOGAT) catalyze the conversion of ammonium and 2-oxoglutarate to glutamine and glutamate. Higher plants have two GOGAT species, ferredoxin-dependent glutamate synthase (Fd-GOGAT) and nicotinamide adenine dinucleotide (NADH)-GOGAT. While Fd-GOGAT participates in the assimilation of ammonium, which is derived from photorespiration in leaves, NADH-GOGAT is highly expressed in roots and its importance needs to be elucidated. While ammonium as a minor nitrogen form in most soils is directly taken up, nitrate as the major nitrogen source needs to be converted to ammonium prior to uptake. The aim of this study was to investigate and quantify the contribution of NADH-GOGAT to the ammonium assimilation in Arabidopsis (Arabidopsis thaliana Columbia) roots. Quantitative real-time polymerase chain reaction (PCR) and protein gel blot analysis showed an accumulation of NADH-GOGAT in response to ammonium supplied to the roots. In addition the localization of NADH-GOGAT and Fd-GOGAT did not fully overlap. Promoter-β-glucuronidase (GUS) fusion analysis and immunohistochemistry showed that NADH-GOGAT was highly accumulated in non-green tissue like vascular bundles, shoot apical meristem, pollen, stigma and roots. Reverse genetic approaches suggested a reduction in glutamate production and biomass accumulation in NADH-GOGAT transfer DNA (T-DNA) insertion lines under normal CO2 condition. The data emphasize the importance of NADH-GOGAT in the ammonium assimilation in Arabidopsis roots. © 2014 Scandinavian Plant Physiology Society.

    DOI: 10.1111/ppl.12177

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  • NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root Reviewed

    Soichi Kojima, Noriyuki Konishi, Marcel Pascal Beier, Keiki Ishiyama, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya

    Plant Signaling and Behavior   9 ( 8 )   2014.6

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    Publishing type:Research paper (scientific journal)  

    © 2014 Landes Bioscience. Higher plants have 2 GOGAT species, Fd-GOGAT and NADHGOGAT. While Fd-GOGAT mainly assimilates ammonium in leaves, which is derived from photorespiration, the function of NADH-GOGAT, which is highly expressed in roots,1 needs to be elucidated. The aim of this study was to clarify the role of NADH-GOGAT in Arabidopsis roots. The supply of ammonium to the roots caused an accumulation of NADH-GOGAT, while Fd-GOGAT 1 and Fd-GOGAT 2 showed no response. A promoter–GUS fusion analysis and immunohistochemistry showed that NADH-GOGAT was located in nongreen tissues like vascular bundles, shoot apical meristem, pollen, stigma, and roots. The localization of NADH-GOGAT and Fd-GOGAT was not overlapped. NADHGOGAT T-DNA insertion lines showed a reduction of glutamate and biomass under normal CO2 conditions. These data emphasizes the importance of NADHGOGAT in the ammonium assimilation of Arabidopsis roots.

    DOI: 10.4161/psb.29402

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Books

  • 百科繚覧 : 若手研究者が挑む学際フロンティア

    東北大学学際科学フロンティア研究所「百科繚覧」編集委員会, 東北大学学際科学フロンティア研究所

    東北大学出版会  2019.1  ( ISBN:9784861633133

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    Total pages:冊   Language:Japanese

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MISC

  • Investigation on the possible involvement of phosphatidic acid in the polar localization of silicon transporter Lsi1 in rice

    KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   64th   2023

  • Identification of a long-distance signaling protein for regulating Si uptake in rice

    YAMAJI Naoki, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   64th   2023

  • Regulation mechanism of boron uptake in rice

    HUANG Sheng, KONISHI Noriyuki, YAMAJI Naoki, MITANI-UENO Namiki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

  • Identification of the Si transporter Lsi3 involved in efficient xylem loading of Si in rice roots

    HUANG Sheng, YAMAJI Naoki, SAKURAI Gen, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

  • Multiple motifs are required for the polar localization of silicon transporter Lsi1 in rice

    KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

  • イネにおけるホウ素吸収の制御機構

    黄勝, 山地直樹, 小西範幸, 邵継鋒, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

  • イネのアンモニウム吸収におけるアンモニウム輸送体1ファミリーの役割

    小西範幸, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

  • Clathrin-mediated endocytosis is not required for the polar localization of mineral transporters in rice

    KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   62nd   2021

  • ケイ酸輸送体Lsi1の極性形成に関わる制御因子の探索

    小西範幸, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

  • ケイ酸輸送体Lsi1の極性分布に関与するモチーフの同定

    小西範幸, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020

  • Role of ammonium transporters in ammonium uptake by rice roots

    KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   61st   2020

  • イネケイ酸輸送体Lsi1の極性分布に関する解析

    小西範幸, MA Jian Feng

    日本農芸化学会大会講演要旨集(Web)   2019   2019

  • ケイ酸輸送体Lsi1の極性制御に関わる領域の同定

    小西範幸, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019

  • Role of N- and C-terminal of rice silicon transporter Lsi1 in its polar localization

    KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   60th   2019

  • シロイヌナズナの窒素利用におけるオーキシン情報伝達の役割の解析

    齋藤雅英, 小西範幸, 菅野圭一, 山谷知行, 小島創一

    日本土壌肥料学会講演要旨集   63   2017

  • 硝酸供給に応答したシロイヌナズナ根の共生細菌群集の変化

    小西範幸, 小西範幸, 大久保卓, 早川俊彦, 山谷知行, 南澤究

    植物微生物研究会研究交流会講演要旨集   26th   2016

  • シロイヌナズナの根におけるアンモニウムの初期同化

    小西範幸, 小西範幸, 石山敬貴, 菅野圭一, 早川俊彦, 山谷知行, 小島創一

    根の研究   25 ( 4 )   2016

  • Analyzing the role of auxin signal transduction in nitrogen utilization of Arabidopsis thaliana

    SAITO Masahide, KONISHI Noriyuki, KANNO Keiichi, KOJIMA Soichi

    日本植物生理学会年会要旨集   57th   2016

  • Cytosolic glutamine synthetase 1;2 dependent ammonium assimilation in Arabidopsis

    KONISHI Noriyuki, ISHIYAMA Keiki, KANNO Keiichi, YAMAYA Tomoyuki, KOJIMA Soichi

    日本植物生理学会年会要旨集   57th   2016

  • 硝酸供給に応じたシロイヌナズナ根の共生細菌群集の変化

    小西範幸, 小西範幸, 早川俊彦, 山谷知行, 南澤究

    植物微生物研究会研究交流会講演要旨集   25th   2015

  • Identification of a specific combination of DNA elements in the promoter regions of ammonium responsive genes in Arabidopsis root

    KONISHI Noriyuki, ISHIYAMA Keiki, MARUYAMA Aoi, SAITO Masahide, MARU Ikumi, HAYAKAWA Toshihiko, YAMAYA Tomoyuki, KOJIMA Soichi

    日本植物生理学会年会要旨集   56th   2015

  • シロイヌナズナは2つの低親和型グルタミン合成酵素を使い分けて根でアンモニウムを同化する

    小西範幸, 石山敬貴, 丸郁美, 松岡香矢, 早川俊彦, 山谷知行, 小島創一

    日本植物生理学会年会要旨集   55th   2014

  • シロイヌナズナNADH-グルタミン酸合成酵素の根におけるアンモニウム同化の重要性

    小西範幸, 松岡香矢, 丸郁美, 早川俊彦, 山谷知行, 小島創一

    日本植物生理学会年会要旨集   54th   2013

  • シロイヌナズナサイトゾル型グルタミン合成酵素1;2は根でアンモニウムによって誘導されアンモニウムを同化する

    小西範幸, 早川俊彦, 山谷知行, 小島創一

    日本農芸化学会大会講演要旨集(Web)   2013   2013

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Research Projects

  • イネアンモニウム輸送体の偏在と小胞体蓄積の生理的意義の解明

    Grant number:22K14805  2022.04 - 2024.03

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    小西 範幸

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

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  • イネ根における4種類のアンモニウム輸送体の機能分担機構の解明

    Grant number:19J00190  2019.04 - 2022.03

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    小西 範幸

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    Grant amount:\9360000 ( Direct expense: \7200000 、 Indirect expense:\2160000 )

    1.これまでに、3種類のAMT1分子種 (AMT1;1, AMT1;2, AMT1;3) が遠心側の細胞膜に極性局在すること、および、ERにも局在することを見出した。しかし、これらの現象が引き起こされるメカニズムや生理的意義は不明なままである。極性局在にはリン酸化が関与しうること、AMT1の活性制御にC末端のリン酸化が関わることが知られていることから、AMT1;2の主要な細胞質領域であるC末端のリン酸化されうる8か所のアミノ酸残基に着目し、細胞内局在と活性の制御におけるリン酸化の影響を検討した。これら8アミノ酸にリン酸化または脱リン酸化を模倣した変異を導入した変異型AMT1;2-Flagをamt1三重変異体に導入し、Flagタグを用いた免疫染色で細胞内局在を、根におけるアンモニウム吸収実験で活性を評価した。これまでに検討したすべての変異型AMT1;2において、極性やER局在への明確な影響は見られなかった。一方、吸収活性に関しては、1アミノ酸の置換によって活性が著しく低下するリン酸化サイトを複数見出した。興味深いことに、1か所以上の残基でリン酸化模倣による活性低下がみられ、少なくとも2か所の残基で脱リン酸化模倣による活性低下がみられた。この結果から、複数のリン酸化と脱リン酸によって、AMT1;2の活性が細やかに調節される可能性が示唆された。
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    2.これまでの解析でいずれの窒素条件でもamt2三重変異体は明確な表現型を示さなかった。amt2三重変異体ではAMT1分子種がAMT2の欠損を機能的に相補すると考え、amt1三重変異体にAMT2分子種の変異を加えた四重、五重、六重変異体を作出した。
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    3. GS1と相互作用する膜タンパク質をGS1;2 promoter::GS1;2-Flagを導入したイネの根を用いた免疫沈降で探索したが、候補となる輸送体を見出すことができなかった。

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