2021/11/30 更新

写真a

シンヤ トモノリ
新屋 友規
SHINYA Tomonori
所属
資源植物科学研究所 准教授
職名
准教授
外部リンク

学位

  • 博士(工学) ( 東京農工大学 )

研究キーワード

  • 生物間相互作用

  • 植物-昆虫間相互作用

  • 植物免疫

  • 分子間相互作用

  • 植食性昆虫

  • 害虫

  • イネ

研究分野

  • ライフサイエンス / 応用分子細胞生物学

  • 環境・農学 / 植物保護科学

  • 環境・農学 / 環境農学

学歴

  • 東京農工大学    

    1999年 - 2004年

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    国名: 日本国

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  • 東京農工大学    

    1995年 - 1999年

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経歴

  • 岡山大学   資源植物科学研究所   准教授

    2019年 - 現在

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  • 岡山大学   資源植物科学研究所   助教

    2013年 - 2018年

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論文

  • Chitooligosaccharide elicitor and oxylipins synergistically elevate phytoalexin production in rice 査読

    Tomonori Shinya, Koji Miyamoto, Kenichi Uchida, Yuko Hojo, Emi Yumoto, Kazunori Okada, Hisakazu Yamane, Ivan Galis

    Plant Molecular Biology   2021年11月

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    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s11103-021-01217-w

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    その他リンク: https://link.springer.com/article/10.1007/s11103-021-01217-w/fulltext.html

  • The rice wound-inducible transcription factor RERJ1 sharing same signal transduction pathway with OsMYC2 is necessary for defense response to herbivory and bacterial blight. 査読 国際誌

    Ioana Valea, Atsushi Motegi, Naoko Kawamura, Koichi Kawamoto, Akio Miyao, Rika Ozawa, Junji Takabayashi, Kenji Gomi, Keiichirou Nemoto, Akira Nozawa, Tatsuya Sawasaki, Tomonori Shinya, Ivan Galis, Koji Miyamoto, Hideaki Nojiri, Kazunori Okada

    Plant molecular biology   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s11103-021-01186-0

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  • Herbivore-induced and constitutive volatiles are controlled by different oxylipin-dependent mechanisms in rice. 査読 国際誌

    Kadis Mujiono, Tilisa Tohi, Islam S Sobhy, Yuko Hojo, Tomonori Shinya, Ivan Galis

    Plant, cell & environment   44 ( 8 )   2687 - 2699   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/pce.14126

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  • Deciphering OPDA Signaling Components in the Momilactone-Producing Moss Calohypnum plumiforme. 査読 国際誌

    Hideo Inagaki, Koji Miyamoto, Noriko Ando, Kohei Murakami, Koki Sugisawa, Shion Morita, Emi Yumoto, Miyu Teruya, Kenichi Uchida, Nobuki Kato, Takuya Kaji, Yousuke Takaoka, Yuko Hojo, Tomonori Shinya, Ivan Galis, Akira Nozawa, Tatsuya Sawasaki, Hideaki Nojiri, Minoru Ueda, Kazunori Okada

    Frontiers in plant science   12   688565 - 688565   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2021.688565

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  • Ethylene functions as a suppressor of volatile production in rice. 査読 国際誌

    Kadis Mujiono, Tilisa Tohi, Islam S Sobhy, Yuko Hojo, Nhan Thanh Ho, Tomonori Shinya, Ivan Galis

    Journal of Experimental Botany   71 ( 20 )   6491 - 6511   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/jxb/eraa341

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  • Silencing of phosphoinositide dependent protein kinase orthologs reduces hypersensitive cell death in Nicotiana benthamiana. 査読

    Akinori Kiba, Kotoko Fukui, Maki Mitani, Ivan Galis, Yuko Hojo, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Plant Biotechnology (Tokyo, Japan)   37 ( 3 )   363 - 367   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Phosphatidic acid plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here we focused on phosphoinositide dependent protein kinases (PDKs) as a candidate required for phosphatidic acid signaling. Based on Arabidopsis PDK sequences, we identified four putative PDK orthologs in N. benthamiana genome. To address the role of PDKs in plant defense responses, we created all four NbPDKs-silenced plants by virus-induced gene silencing. the NbPDKs-silenced plants showed a moderately reduced growth phenotype. Induction of hypersensitive cell death was compromised in the NbPDKs-silenced plants challenged with Ralstonia solanacearum. The hypersensitive cell death induced by bacterial effectors was also reduced in the NbPDKs-silenced plants. the NbPDKs-silenced plants showed decreased production of salicylic acid, jasmonic acid and jasmonoyl-L-isoleucine, as well as hydrogen peroxide after inoculation with R. solanacearum. These results suggest that NbPDKs might have an important role in the regulation of the hypersensitive cell death via plant hormone signaling and oxidative burst.

    DOI: 10.5511/plantbiotechnology.20.0511b

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  • Nonglandular silicified trichomes are essential for rice defense against chewing herbivores 査読

    Joackin B. Andama, Kadis Mujiono, Yuko Hojo, Tomonori Shinya, Ivan Galis

    Plant, Cell & Environment   43 ( 9 )   2019 - 2032   2020年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/pce.13775

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.13775

  • Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death. 査読 国際誌

    Wenxiu Ye, Shintaro Munemasa, Tomonori Shinya, Wei Wu, Tao Ma, Jiang Lu, Toshinori Kinoshita, Hanae Kaku, Naoto Shibuya, Yoshiyuki Murata

    Proceedings of the National Academy of Sciences of the United States of America   117 ( 34 )   20932 - 20942   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Many pathogenic fungi exploit stomata as invasion routes, causing destructive diseases of major cereal crops. Intensive interaction is expected to occur between guard cells and fungi. In the present study, we took advantage of well-conserved molecules derived from the fungal cell wall, chitin oligosaccharide (CTOS), and chitosan oligosaccharide (CSOS) to study how guard cells respond to fungal invasion. In Arabidopsis, CTOS induced stomatal closure through a signaling mediated by its receptor CERK1, Ca2+, and a major S-type anion channel, SLAC1. CSOS, which is converted from CTOS by chitin deacetylases from invading fungi, did not induce stomatal closure, suggesting that this conversion is a fungal strategy to evade stomatal closure. At higher concentrations, CSOS but not CTOS induced guard cell death in a manner dependent on Ca2+ but not CERK1. These results suggest that stomatal immunity against fungal invasion comprises not only CTOS-induced stomatal closure but also CSOS-induced guard cell death.

    DOI: 10.1073/pnas.1922319117

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  • Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana. 査読 国際誌

    Akinori Kiba, Masahito Nakano, Miki Hosokawa, Ivan Galis, Hiroko Nakatani, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Journal of Experimental Botany   71 ( 16 )   5027 - 5038   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Phospholipid signaling plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here, we isolated two phospholipase C2 (PLC2) orthologs in the N. benthamiana genome, designated as PLC2-1 and 2-2. Both NbPLC2-1 and NbPLC2-2 were expressed in most tissues and were induced by infiltration with bacteria and flg22. NbPLC2-1 and NbPLC2-2 (NbPLC2s) double-silenced plants showed a moderately reduced growth phenotype. The induction of the hypersensitive response was not affected, but bacterial growth and the appearance of bacterial wilt were accelerated in NbPLC2s-silenced plants when they were challenged with a virulent strain of Ralstonia solanacearum that was compatible with N. benthamiana. NbPLC2s-silenced plants showed reduced expression levels of NbPR-4, a marker gene for jasmonic acid signaling, and decreased jasmonic acid and jasmonoyl-L-isoleucine contents after inoculation with R. solanacearum. The induction of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) marker genes was reduced in NbPLC2s-silenced plants after infiltration with R. solanacearum or Pseudomonas fluorescens. Accordingly, the resistance induced by flg22 was compromised in NbPLC2s-silenced plants. In addition, the expression of flg22-induced PTI marker genes, the oxidative burst, stomatal closure, and callose deposition were all reduced in the silenced plants. Thus, NbPLC2s might have important roles in pre- and post-invasive defenses, namely in the induction of PTI.

    DOI: 10.1093/jxb/eraa233

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  • Affinity Labeling and Purification of Plant Chitin-Binding LysM Receptor with Chitin Octasaccharide Derivatives. 国際誌

    Tomonori Shinya, Naoto Shibuya, Hanae Kaku

    Methods in molecular biology (Clifton, N.J.)   2132   401 - 412   2020年

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    記述言語:英語  

    Lysin motif (LysM) is a carbohydrate-binding modules found in all kingdoms. LysM binds to N-acetylglucosamine-containing molecules such as peptidoglycan, chitin, Nod factor, and Myc factor and is found in peptidoglycan hydrolases, chitinases, and plant pathogen effectors and plant receptor/co-receptor for defense and symbiosis signaling. This chapter describes the synthesis of a nonradioactive chitin ligand, biotinylated chitin octasaccharide, (GlcNAc)8-Bio, and its application for the detection and characterization of chitin-binding LysM receptor CEBiP in the microsomal membrane fraction of rice suspension-cultured cells by affinity labeling. We also describe the purification of CEBiP from the plasma membrane of the rice cells by affinity chromatography with the synthesized (GlcNAc)8-APEA-CH-Sepharose as an affinity matrix.

    DOI: 10.1007/978-1-0716-0430-4_39

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  • Brown planthopper honeydew-associated symbiotic microbes elicit momilactones in rice 査読

    David Wari, Kabir Md Alamgir, Kadis Mujiono, Yuko Hojo, Akio Tani, Tomonori Shinya, Hiroko Nakatani, Ivan Galis

    Plant Signaling & Behavior   14 ( 11 )   1655335 - 1655335   2019年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa UK Limited  

    DOI: 10.1080/15592324.2019.1655335

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  • Honeydew-associated microbes elicit defense responses against brown planthopper in rice. 査読 国際誌

    David Wari, Md Alamgir Kabir, Kadis Mujiono, Yuko Hojo, Tomonori Shinya, Akio Tani, Hiroko Nakatani, Ivan Galis

    Journal of experimental botany   70 ( 5 )   1683 - 1696   2019年3月

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    記述言語:英語  

    Feeding of sucking insects, such as the rice brown planthopper (Nilaparvata lugens; BPH), causes only limited mechanical damage on plants that is otherwise essential for injury-triggered defense responses against herbivores. In pursuit of complementary BPH elicitors perceived by plants, we examined the potential effects of BPH honeydew secretions on the BPH monocot host, rice (Oryza sativa). We found that BPH honeydew strongly elicits direct and putative indirect defenses in rice, namely accumulation of phytoalexins in the leaves, and release of volatile organic compounds from the leaves that serve to attract natural enemies of herbivores, respectively. We then examined the elicitor active components in the honeydew and found that bacteria in the secretions are responsible for the activation of plant defense. Corroborating the importance of honeydew-associated microbiota for induced plant resistance, BPHs partially devoid of their microbiota via prolonged antibiotics ingestion induced significantly less defense in rice relative to antibiotic-free insects applied to similar groups of plants. Our data suggest that rice plants may additionally perceive herbivores via their honeydew-associated microbes, allowing them to discriminate between incompatible herbivores-that do not produce honeydew-and those that are compatible and therefore dangerous.

    DOI: 10.1093/jxb/erz041

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  • Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice. 査読 国際誌

    Tomonori Shinya, Shigetaka Yasuda, Kiwamu Hyodo, Rena Tani, Yuko Hojo, Yuka Fujiwara, Kei Hiruma, Takuma Ishizaki, Yasunari Fujita, Yusuke Saijo, Ivan Galis

    The Plant journal : for cell and molecular biology   94 ( 4 )   626 - 637   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Plant defense against herbivores is modulated by herbivore-associated molecular patterns (HAMPs) from oral secretions (OS) and/or saliva of insects. Furthermore, feeding wounds initiate plant self-damage responses modulated by danger-associated molecular patterns (DAMPs) such as immune defense-promoting plant elicitor peptides (Peps). While temporal and spatial co-existence of both patterns during herbivory implies a possibility of their close interaction, the molecular mechanisms remain undetermined. Here we report that exogenous application of rice (Oryza sativa) peptides (OsPeps) can elicit multiple defense responses in rice cell cultures. Specific activation of OsPROPEP3 gene transcripts in rice leaves by wounding and OS treatments further suggests a possible involvement of the OsPep3 peptide in rice-herbivore interactions. Correspondingly, we found that simultaneous application of OsPep3 and Mythimna loreyi OS significantly amplifies an array of defense responses in rice cells, including mitogen-activated protein kinase activation, and generation of defense-related hormones and metabolites. The induction of OsPROPEP3/4 by OsPep3 points to a positive auto-feedback loop in OsPep signaling which may contribute to additional enhancement of defense signal(s). Finally, the overexpression of the OsPep receptor OsPEPR1 increases the sensitivity of rice plants not only to the cognate OsPeps but also to OS signals. Our findings collectively suggest that HAMP-DAMP signal integration provides a critical step in the amplification of defense signaling in plants.

    DOI: 10.1111/tpj.13883

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  • Phosphoinositide 3-kinase participates in L-methionine sulfoximine-induced cell death via salicylic acid mediated signaling in Nicotiana benthamiana 査読

    Sayuri Sumida, Makoto Ito, Ivan Galis, Hiroko Nakatani, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba

    Journal of Plant Physiology   218   167 - 170   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier GmbH  

    Pseudomonas syringae pv. tabaci causes wildfire disease by the action of tabtoxinine-β-lactam (TβL), a non-specific bacterial toxin. To better understand the molecular mechanisms of wildfire disease and its development, we focused on the phosphoinositide 3-kinase in Nicotiana benthamiana (NbPI3K) and its potential role in the disease outbreak, using L-methionine sulfoximine (MSX) as an easily accessible mimic of the TβL action. The NbPI3K-silenced plants showed accelerated induction of cell death and necrotic lesion formation by MSX, and the expression of hin1, marker gene for the programmed cell death, was strongly induced in the plants. However, the accumulation of ammonium ions, caused by MSX inhibition of glutamine sythetase activity, was not affected by the NbPI3K-silencing. Interestingly, the expression of PR-1a, a marker gene for salicylic acid (SA) innate immunity signaling, and accumulation of SA were both enhanced in the NbPI3K-silenced plants. Accordingly, the acceleration of MSX-induced cell death by NbPI3K-silencing was reduced in NahG plants, and by double silencing of NbPI3K together with the NbICS1 encoding a SA-biosynthetic enzyme. As silencing of NbPI3K accelerated the TβL-induced necrotic lesions, and lesions of wildfire disease caused by P. syringae pv. tabaci, these results suggest that the NbPI3K-related pathway might act as a negative regulator of cell death during development of wildfire disease that involves SA-dependent signaling pathway downstream of TβL action in N. benthamiana.

    DOI: 10.1016/j.jplph.2017.07.016

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  • Oral Secretions Affect HIPVs Induced by Generalist (Mythimna loreyi) and Specialist (Parnara guttata) Herbivores in Rice. 査読 国際誌

    Islam S Sobhy, Atsushi Miyake, Tomonori Shinya, Ivan Galis

    Journal of chemical ecology   43 ( 9 )   929 - 943   2017年9月

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    記述言語:英語  

    Plants synthesize variable mixtures of herbivore-induced plant volatiles (HIPVs) as part of their evolutionary conserved defense. To elucidate the impact of chewing herbivores with different level of adaptation on HIPV profiles in rice, we measured HIPVs released from rice seedlings challenged by either the generalist herbivore Mythimna loreyi (MYL) or the specialist Parnara guttata (PAG). Both herbivores markedly elicited the emission of HIPVs, mainly on the second and third days after attack compared to control plants. In addition, side-by-side HIPV comparisons using MYL and PAG caterpillars revealed that generalist feeding induced comparably more HIPVs relative to specialist, particularly on day two as highlighted by multivariate analysis (PLS-DA) of emitted HIPVs, and further confirmed in mimicked herbivory experiments. Here, mechanically wounded plants treated with water (WW) released more VOCs than untreated controls, and on top of this, oral secretions (OS) from both herbivores showed differential effects on volatile emissions from the wounded plants. Similar to actual herbivory, MYL OS promoted higher amounts of HIPVs relative to PAG OS, thus supporting disparate induction of rice indirect defenses in response to generalist and specialist herbivores, which could be due to the differential composition of their OS. (196 words).

    DOI: 10.1007/s10886-017-0882-4

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  • Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants 査読

    Kimiaki Tanabe, Yuko Hojo, Tomonori Shinya, Ivan Galis

    JOURNAL OF INTEGRATIVE PLANT BIOLOGY   58 ( 11 )   903 - 913   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Two phenolamides (PAs), p-coumaroylputrescine and feruloylputrescine strongly accumulate in rice (Oryza sativa cv. Nipponbare) leaves subjected to attack of chewing and sucking herbivores. Here we identified and characterized in vitro three novel rice genes that mediated coumaroyl-CoA/feruloyl-CoA conjugation to polyamines, putrescine and agmatine. Interestingly, two genes were highly specific for their polyamine substrates, encoding putrescine N-hydroxycinnamoyltransferase and agmatine N-hydroxycinnamoyltransferase, while the third enzyme could use both polyamines and it was therefore annotated as putrescine/agmatine N-hydroxycinnamoyltransferase. All genes were preferentially expressed in rice roots and developing flowers, and in addition, the putrescine/agmatine N-hydroxycinnamoyltransferase transcripts were strongly induced by wounding in the young rice leaves. Because the wound response of this gene was only partially suppressed in the jasmonoyl-L-isoleucine deficient plants (Osjar1), it suggests that its upregulation (as well as inducible PAs in rice) may be largely independent of jasmonoyl-L-isoleucine signaling pathway. The finding of three closely related genes with a similar and/or overlapping activity in PA biosynthesis provides another striking example of rapid diversification of plant metabolism in response to environmental stresses in nature.

    DOI: 10.1111/jipb.12480

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  • Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice 査読

    Tomonori Shinya, Yuko Hojo, Yoshitake Desaki, John T. Christeller, Kazunori Okada, Naoto Shibuya, Ivan Galis

    SCIENTIFIC REPORTS   6   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Induced plant defense responses against insect herbivores are triggered by wounding and/or perception of herbivore elicitors from their oral secretions (OS) and/or saliva. In this study, we analyzed OS isolated from two rice chewing herbivores, Mythimna loreyi and Parnara guttata. Both types of crude OS had substantial elicitor activity in rice cell system that allowed rapid detection of early and late defense responses, i.e. accumulation of reactive oxygen species (ROS) and defense secondary metabolites, respectively. While the OS from M. loreyi contained large amounts of previously reported insect elicitors, fatty acid-amino acid conjugates (FACs), the elicitor-active P. guttata's OS contained no detectable FACs. Subsequently, elicitor activity associated with the high molecular mass fraction in OS of both herbivores was identified, and shown to promote ROS and metabolite accumulations in rice cells. Notably, the application of N-linolenoyl-Gln (FAC) alone had only negligible elicitor activity in rice cells; however, the activity of isolated elicitor fraction was substantially promoted by this FAC. Our results reveal that plants integrate various independent signals associated with their insect attackers to modulate their defense responses and reach maximal fitness in nature.

    DOI: 10.1038/srep32537

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  • Colletotrichum higginsianum extracellular LysM proteins play dual roles in appressorial function and suppression of chitin-triggered plant immunity 査読

    Hiroyuki Takahara, Stephane Hacquard, Anja Kombrink, H. Bleddyn Hughes, Vivek Halder, Guillaume P. Robin, Kei Hiruma, Ulla Neumann, Tomonori Shinya, Erich Kombrink, Naoto Shibuya, Bart P. H. J. Thomma, Richard J. O'Connell

    NEW PHYTOLOGIST   211 ( 4 )   1323 - 1337   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    The genome of the hemibiotrophic anthracnose fungus, Colletotrichum higginsianum, encodes a large repertoire of candidate-secreted effectors containing LysM domains, but the role of such proteins in the pathogenicity of any Colletotrichum species is unknown. Here, we characterized the function of two effectors, ChELP1 and ChELP2, which are transcriptionally activated during the initial intracellular biotrophic phase of infection. Using immunocytochemistry, we found that ChELP2 is concentrated on the surface of bulbous biotrophic hyphae at the interface with living host cells but is absent from filamentous necrotrophic hyphae. We show that recombinant ChELP1 and ChELP2 bind chitin and chitin oligomers invitro with high affinity and specificity and that both proteins suppress the chitin-triggered activation of two immune-related plant mitogen-activated protein kinases in the host Arabidopsis. Using RNAi-mediated gene silencing, we found that ChELP1 and ChELP2 are essential for fungal virulence and appressorium-mediated penetration of both Arabidopsis epidermal cells and cellophane membranes invitro. The findings suggest a dual role for these LysM proteins as effectors for suppressing chitin-triggered immunity and as proteins required for appressorium function.

    DOI: 10.1111/nph.13994

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  • Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory 査読

    Kabir Md Alamgir, Yuko Hojo, John T. Christeller, Kaori Fukumoto, Ryutaro Isshiki, Tomonori Shinya, Ian T. Baldwin, Ivan Galis

    PLANT CELL AND ENVIRONMENT   39 ( 2 )   453 - 466   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Plants defend against attack from herbivores by direct and indirect defence mechanisms mediated by the accumulation of phytoalexins and release of volatile signals, respectively. While the defensive arsenals of some plants, such as tobacco and Arabidopsis are well known, most of rice's (Oryza sativa) defence metabolites and their effectiveness against herbivores remain uncharacterized. Here, we used a non-biassed metabolomics approach to identify many novel herbivory-regulated metabolic signatures in rice. Most were up-regulated by herbivore attack while only a few were suppressed. Two of the most prominent up-regulated signatures were characterized as phenolamides (PAs), p-coumaroylputrescine and feruloylputrescine. PAs accumulated in response to attack by both chewing insects, i. e. feeding of the lawn armyworm (Spodoptera mauritia) and the rice skipper (Parnara guttata) larvae, and the attack of the sucking insect, the brown planthopper (Nilaparvata lugens, BPH). In bioassays, BPH insects feeding on 15% sugar solution containing p-coumaroylputrescine or feruloylputrescine, at concentrations similar to those elicited by heavy BPH attack in rice, had a higher mortality compared to those feeding on sugar diet alone. Our results highlight PAs as a rapidly expanding new group of plant defence metabolites that are elicited by herbivore attack, and deter herbivores in rice and other plants.

    DOI: 10.1111/pce.12640

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  • Silencing of Nicotiana benthamiana SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against Pseudomonas syringae 査読

    Akinori Kiba, Yu Imanaka, Masahito Nakano, Ivan Galis, Yuko Hojo, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Plant Biotechnology   33 ( 2 )   111 - 115   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    © 2016 The Japanese Society for Plant Cell and Molecular Biology. We previously identified SEC14, phospholipid transfer protein superfamily gene, in Nicotiana benthamiana (NbSEC14) that was closely related to phospholipid signaling as well as jasmonic acid-dependent defense responses during plant immune responses against Ralstonia solanacearum. To examine effect of NbSEC14-silencing on basal plant defenses, we used two other bacterial pathogens with different virulent strategies, Pseudomonas syringae pv. tabaci and pv. mellea. NbSEC14-silenced plants showed accelerated growth of P. syringae pv. tabaci and pv. mellea, and formation of necrotic lesions. Induction of JA-related PR-4 gene was compromised in NbSEC14-silenced plants, which was supported by reduced jasmonic acid levels in NbSEC14-silenced plants. These results suggested that NbSEC14 might be regulating plant basal resistance against plant pathogenic Pseudomonads via jasmonic acid-dependent signaling pathway.

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  • Chitin-mediated plant-fungal interactions: catching, hiding and handshaking 査読

    Tomonori Shinya, Tomomi Nakagawa, Hanae Kaku, Naoto Shibuya

    CURRENT OPINION IN PLANT BIOLOGY   26   64 - 71   2015年8月

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    記述言語:英語   出版者・発行元:CURRENT BIOLOGY LTD  

    Plants can detect infecting fungi through the perception of chitin oligosaccharides by lysin motif receptors such as CEBiP and CERK1. A major function of CERK1 seems to be as a signaling molecule in the receptor complex formed with ligand-binding molecules and to activate downstream defense signaling. Fungal pathogens, however, have developed counter strategies to escape from the chitin-mediated detection by using effectors and/or changing their cell walls. Common structural features between chitin and Nod-/Mycfactors and corresponding receptors have suggested the close relationships between the chitin-mediated immunity and rhizobial/arbuscular mycorrhizal symbiosis. The recent discovery of the dual function of OsCERK1 in both plant immunity and mycorrhizal symbiosis sheds new light on the evolutionary relationships between defense and symbiotic systems in plants.

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  • Uptake of chitosan-derived D-glucosamine oligosaccharides in Streptomyces coelicolor A3(2) 査読

    Pascal Viens, Marie-Pierre Dubeau, Akane Kimura, Yoshitake Desaki, Tomonori Shinya, Naoto Shibuya, Akihiro Saito, Ryszard Brzezinski

    FEMS MICROBIOLOGY LETTERS   362 ( 9 )   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The csnR gene, localized at the beginning of an operon, csnR-K, which organization is conserved through many actinomycete genomes, was previously shown to repress the transcription of the chitosanase gene csnA in Streptomyces lividans. However, knowledge on the function of the whole csnR-K operon in the metabolism of chitosan (an N-deacetylated derivative of chitin) remained limited. Mutants of S. coelicolor A3(2) harboring partial or total deletions of the csnR-K operon were analyzed for their capacity to uptake glucosamine oligosaccharides (GlcN)(n). The csnR-K operon was autoregulated by CsnR repressor and its transcription was inducible by GlcN oligosaccharides. The operon controlled the uptake of GlcN oligosaccharides in S. coelicolor A3(2), with a minor contribution to the consumption of monomeric GlcN but not chitin-related N-acetylated derivatives. The deletion of the whole operon abolished the uptake of GlcN oligosaccharides. The CsnEFG transporter encoded by this operon is the front door for the assimilation of chitosan-derived hydrolysis products in S. coelicolor A3(2). The ATP-binding component MsiK was essential for CsnEFG transport function. Also, deletion of msiK abolished the induction of csnA transcription by GlcN oligosaccharides.

    DOI: 10.1093/femsle/fnv048

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  • Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27 査読

    Tomonori Shinya, Koji Yamaguchi, Yoshitake Desaki, Kenta Yamada, Tomoko Narisawa, Yoshihiro Kobayashi, Kanako Maeda, Maruya Suzuki, Takumi Tanimoto, Jun Takeda, Masato Nakashima, Ryota Funama, Mari Narusaka, Yoshihiro Narusaka, Hanae Kaku, Tsutomu Kawasaki, Naoto Shibuya

    PLANT JOURNAL   79 ( 1 )   56 - 66   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Recognition of microbe-associated molecular patterns (MAMPs) initiates pattern-triggered immunity in host plants. Pattern recognition receptors (PRRs) and receptor-like cytoplasmic kinases (RLCKs) are the major components required for sensing and transduction of these molecular patterns. However, the regulation of RLCKs by PRRs and their specificity remain obscure. In this study we show that PBL27, an Arabidopsis ortholog of OsRLCK185, is an immediate downstream component of the chitin receptor CERK1 and contributes to the regulation of chitin-induced immunity in Arabidopsis. Knockout of PBL27 resulted in the suppression of several chitin-induced defense responses, including the activation of MPK3/6 and callose deposition as well as in disease resistance against fungal and bacterial infections. On the other hand, the contribution of PBL27 to flg22 signaling appears to be very limited, suggesting that PBL27 selectively regulates defense signaling downstream of specific PRR complexes. In vitro phosphorylation experiments showed that CERK1 preferentially phosphorylated PBL27 in comparison to BIK1, whereas phosphorylation of PBL27 by BAK1 was very low compared with that of BIK1. Thus, the substrate specificity of the signaling receptor-like kinases, CERK1 and BAK1, may determine the preference of downstream RLCKs.

    DOI: 10.1111/tpj.12535

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  • Presence of LYM2 dependent but CERK1 independent disease resistance in Arabidopsis 査読

    Yoshihiro Narusaka, Tomonori Shinya, Mari Narusaka, Noriko Motoyama, Hikaru Shimada, Kosuke Murakami, Naoto Shibuya

    Plant Signaling and Behavior   8 ( 9 )   e25345   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Plants have the ability to detect invading fungi through the perception of chitin fragments released from the fungal cell walls. Plant chitin receptor consists of two types of plasma membrane proteins, CEBiP and CERK1. However, the contribution of these proteins to chitin signaling is different between Arabidopsis and rice. In Arabidopsis, it seems CERK1 receptor kinase is enough for both ligand perception and signaling, whereas both CEBiP and OsCERK1 are required for chitin signaling in rice. Here we report that Arabidopsis CEBiP homolog, LYM2, is not involved in chitin signaling but contributes to resistance against a fungal pathogen, Alternaria brassicicola, indicating the presence of a novel disease resistance mechanism in Arabidopsis. © 2013 Landes Bioscience.

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  • Functional Characterization of CEBiP and CERK1 Homologs in Arabidopsis and Rice Reveals the Presence of Different Chitin Receptor Systems in Plants 査読

    Tomonori Shinya, Noriko Motoyama, Asahi Ikeda, Miyuki Wada, Kota Kamiya, Masahiro Hayafune, Hanae Kaku, Naoto Shibuya

    PLANT AND CELL PHYSIOLOGY   53 ( 10 )   1696 - 1706   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Chitin is a representative microbe-associated molecular pattern (MAMP) molecule for various fungi and induces immune responses in many plant species. It has been clarified that the chitin signaling in rice requires a receptor kinase OsCERK1 and a receptor-like protein (Os)CEBiP, which specifically binds chitin oligosaccharides. On the other hand, Arabidopsis requires a receptor kinase (At)CERK1 for chitin signaling but it is not clear whether the plant also requires a CEBiP-like molecule for chitin perception/signaling. To clarify the similarity/difference of the chitin receptor in these two model plants, we first characterized CEBiP homologs in Arabidopsis. Only one of three CEBiP homologs, AtCEBiP (LYM2), showed a high-affinity binding for chitin oligosaccharides similar to rice CEBiP. AtCEBiP also represented the major chitin-binding protein in the Arabidopsis membrane. However, the single/triple knockout (KO) mutants of Arabidopsis CEBiP homologs and the overexpressor of AtCEBiP showed chitin-induced defense responses similar to wild-type Arabidopsis, indicating that AtCEBiP is biochemically functional as a chitin-binding protein but does not contribute to signaling. Studies of the chitin binding properties of the ectodomains of At/OsCERK1 and the chimeric receptors consisting of ecto/cytosolic domains of these molecules indicated that AtCERK1 is sufficient for chitin perception by itself.

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  • Effector-Mediated Suppression of Chitin-Triggered Immunity by Magnaporthe oryzae Is Necessary for Rice Blast Disease 査読

    Thomas A. Mentlak, Anja Kombrink, Tomonori Shinya, Lauren S. Ryder, Ippei Otomo, Hiromasa Saitoh, Ryohei Terauchi, Yoko Nishizawa, Naoto Shibuya, Bart P. H. J. Thomma, Nicholas J. Talbot

    PLANT CELL   24 ( 1 )   322 - 335   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Plants use pattern recognition receptors to defend themselves from microbial pathogens. These receptors recognize pathogen-associated molecular patterns (PAMPs) and activate signaling pathways that lead to immunity. In rice (Oryza sativa), the chitin elicitor binding protein (CEBiP) recognizes chitin oligosaccharides released from the cell walls of fungal pathogens. Here, we show that the rice blast fungus Magnaporthe oryzae overcomes this first line of plant defense by secreting an effector protein, Secreted LysM Protein1 (Slp1), during invasion of new rice cells. We demonstrate that Slp1 accumulates at the interface between the fungal cell wall and the rice plasma membrane, can bind to chitin, and is able to suppress chitin-induced plant immune responses, including generation of reactive oxygen species and plant defense gene expression. Furthermore, we show that Slp1 competes with CEBiP for binding of chitin oligosaccharides. Slp1 is required by M. oryzae for full virulence and exerts a significant effect on tissue invasion and disease lesion expansion. By contrast, gene silencing of CEBiP in rice allows M. oryzae to cause rice blast disease in the absence of Slp1. We propose that Slp1 sequesters chitin oligosaccharides to prevent PAMP-triggered immunity in rice, thereby facilitating rapid spread of the fungus within host tissue.

    DOI: 10.1105/tpc.111.092957

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  • Biochemical and phylogenetic analysis of CEBiP-like LysM domain-containing extracellular proteins in higher plants. 査読 国際誌

    Judith Fliegmann, Sandra Uhlenbroich, Tomonori Shinya, Yves Martinez, Benoit Lefebvre, Naoto Shibuya, Jean-Jacques Bono

    Plant physiology and biochemistry : PPB   49 ( 7 )   709 - 20   2011年7月

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    記述言語:英語  

    The chitin elicitor-binding protein (CEBiP) from rice was the first plant lysin motif (LysM) protein for which the biological and biochemical function had been established. It belongs to a plant-specific family of extracellular LysM proteins (LYMs) for which we analyzed the phylogeny. LYMs are present in vascular plants only, where an early gene duplication event might have resulted in two types which were retained in present day genomes. LYMs consist of a signal peptide, three consecutive LysMs, separated by cysteine pairs, and a C-terminal region without any known signature, whose length allows the distinction between the two types, and which may be followed by a glycosylphosphatidylinositol (GPI) anchor motif. We analyzed a representative of each type, MtLYM1 and MtLYM2, from Medicago truncatula at the biochemical level and with respect to their expression patterns and observed some similarities but also marked differences. MtLYM1 and MtLYM2 proved to be very different with regard to abundance and apparent molecular mass on SDS-PAGE. Both undergo several post-translational modifications, including N-glycosylation and the addition of a GPI anchor, which would position the proteins at the outer face of the plasma membrane. Only MtLYM2, but not MtLYM1, showed specific binding to biotinylated N-acetylchitooctaose in a manner similar to CEBiP, which belongs to the same type. We postulate that LYM2-type proteins likely function in the perception of chitin-related molecules, whereas possible functions of LYM1-type proteins remain to be elucidated.

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  • Conserved Fungal LysM Effector Ecp6 Prevents Chitin-Triggered Immunity in Plants 査読

    Ronnie de Jonge, H. Peter van Esse, Anja Kombrink, Tomonori Shinya, Yoshitake Desaki, Ralph Bours, Sander van der Krol, Naoto Shibuya, Matthieu H. A. J. Joosten, Bart P. H. J. Thomma

    SCIENCE   329 ( 5994 )   953 - 955   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC ADVANCEMENT SCIENCE  

    Multicellular organisms activate immunity upon recognition of pathogen-associated molecular patterns (PAMPs). Chitin is the major component of fungal cell walls, and chitin oligosaccharides act as PAMPs in plant and mammalian cells. Microbial pathogens deliver effector proteins to suppress PAMP-triggered host immunity and to establish infection. Here, we show that the LysM domain-containing effector protein Ecp6 of the fungal plant pathogen Cladosporium fulvum mediates virulence through perturbation of chitin-triggered host immunity. During infection, Ecp6 sequesters chitin oligosaccharides that are released from the cell walls of invading hyphae to prevent elicitation of host immunity. This may represent a common strategy of host immune suppression by fungal pathogens, because LysM effectors are widely conserved in the fungal kingdom.

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  • Glyco-conjugates as elicitors or suppressors of plant innate immunity 査読

    Alba Silipo, Gitte Erbs, Tomonori Shinya, J. Maxwell Dow, Michelangelo Parrilli, Rosa Lanzetta, Naoto Shibuya, Mari-Anne Newman, Antonio Molinaro

    GLYCOBIOLOGY   20 ( 4 )   406 - 419   2010年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS INC  

    Innate immunity is the first line of defense against invading microorganisms in vertebrates and the only line of defense in invertebrates and plants. Bacterial glyco-conjugates, such as lipopolysaccharides (LPS) from the outer membrane of Gram-negative bacteria and peptidoglycan (PGN) from the cell walls of both Gram-positive and Gram-negative bacteria, and fungal and oomycete glycoconjugates such as oligosaccharides derived from the cell wall components beta-glucan, chitin and chitosan, have been found to act as elicitors of plant innate immunity. These conserved indispensable microbe-specific molecules are also referred to as microbe-associated molecular patterns (MAMPs). Other glyco-conjugates such as bacterial extracellular polysaccharides (EPS) and cyclic glucan have been shown to suppress innate immune responses, thus conversely promoting pathogenesis. MAMPs are recognized by the plant innate immune system though the action of pattern recognition receptors (PRRs). A greater insight into the mechanisms of MAMP recognition and the description of PRRs for different microbial glyco-conjugates will have considerable impact on the improvement of plant health and disease resistance. Here we review the current knowledge about the bacterial MAMPs LPS and PGN, the fungal MAMPs beta-glucan, chitin and chitosan oligosaccharides and the bacterial suppressors EPS and cyclic glucan, with particular reference to the chemical structures of these molecules, the PRRs involved in their recognition (where these have been defined), and possible mechanisms underlying suppression.

    DOI: 10.1093/glycob/cwp201

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  • Characterization of receptor proteins using affinity cross-linking with biotinylated ligands. 査読

    Tomonori Shinya, Tomohiko Osada, Yoshitake Desaki, Masahiro Hatamoto, Yuko Yamanaka, Hisashi Hirano, Ryota Takai, Fang-Sik Che, Hanae Kaku, Naoto Shibuya

    Plant & cell physiology   51 ( 2 )   262 - 70   2010年2月

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    記述言語:英語  

    The plant genome encodes a wide range of receptor-like proteins but the function of most of these proteins is unknown. We propose the use of affinity cross-linking of biotinylated ligands for a ligand-based survey of the corresponding receptor molecules. Biotinylated ligands not only enable the analysis of receptor-ligand interactions without the use of radioactive compounds but also the isolation and identification of receptor molecules by a simple affinity trapping method. We successfully applied this method for the characterization, isolation and identification of the chitin elicitor binding protein (CEBiP). A biocytin hydrazide conjugate of N-acetylchitooctaose (GN8-Bio) was synthesized and used for the detection of CEBiP in the plasma or microsomal membrane preparations from rice and carrot cells. Binding characteristics of CEBiP analyzed by inhibition studies were in good agreement with the previous results obtained with the use of a radiolabeled ligand. The biotin-tagged CEBiP could be purified by avidin affinity chromatography and identified by LC-MALDI-MS/MS after tryptic digestion. We also used this method to detect OsFLS2, a rice receptor-like kinase for the perception of the peptide elicitor flg22, in membrane preparations from rice cells overexpressing OsFLS2. This work demonstrates the applicability of this method to the purification and identification of plant receptor proteins.

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  • Suppression of a phospholipase D gene, OsPLDbeta1, activates defense responses and increases disease resistance in rice. 査読 国際誌

    Takeshi Yamaguchi, Masaharu Kuroda, Hiromoto Yamakawa, Taketo Ashizawa, Kazuyuki Hirayae, Leona Kurimoto, Tomonori Shinya, Naoto Shibuya

    Plant physiology   150 ( 1 )   308 - 19   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Phospholipase D (PLD) plays an important role in plants, including responses to abiotic as well as biotic stresses. A survey of the rice (Oryza sativa) genome database indicated the presence of 17 PLD genes in the genome, among which OsPLDalpha1, OsPLDalpha5, and OsPLDbeta1 were highly expressed in most tissues studied. To examine the physiological function of PLD in rice, we made knockdown plants for each PLD isoform by introducing gene-specific RNA interference constructs. One of them, OsPLDbeta1-knockdown plants, showed the accumulation of reactive oxygen species in the absence of pathogen infection. Reverse transcription-polymerase chain reaction and DNA microarray analyses revealed that the knockdown of OsPLDbeta1 resulted in the up-/down-regulation of more than 1,400 genes, including the induction of defense-related genes such as pathogenesis-related protein genes and WRKY/ERF family transcription factor genes. Hypersensitive response-like cell death and phytoalexin production were also observed at a later phase of growth in the OsPLDbeta1-knockdown plants. These results indicated that the OsPLDbeta1-knockdown plants spontaneously activated the defense responses in the absence of pathogen infection. Furthermore, the OsPLDbeta1-knockdown plants exhibited increased resistance to the infection of major pathogens of rice, Pyricularia grisea and Xanthomonas oryzae pv oryzae. These results suggested that OsPLDbeta1 functions as a negative regulator of defense responses and disease resistance in rice.

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  • The msiK gene, encoding the ATP-hydrolysing component of N,N '-diacetylchitobiose ABC transporters, is essential for induction of chitinase production in Streptomyces coelicolor A3(2) 査読

    Akihiro Saito, Takeshi Fujii, Tomonori Shinya, Naoto Shibuya, Akikazu Ando, Kiyotaka Miyashita

    MICROBIOLOGY-SGM   154 ( Pt 11 )   3358 - 3365   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The dasABC genes encode an ATP-binding cassette (ABC) transporter, which is one of the uptake systems for N,N'-diacetylchitobiose [(GlcNAC)(2)] in Streptomyces coelicolor A3(2), although the gene encoding the ABC subunit that provides ATP hydrolysis for DasABC has not been identified. In this study, we disrupted the sequence that is highly homologous to the msiK gene, the product of which is an ABC subunit assisting several ABC permeases in other Streptomyces species. Disruption of msiK severely affected the ability of S. coelicolor A3(2) to utilize maltose, cellobiose, starch, cellulose, chitin and chitosan, but not glucose. The msiK null mutant lacked (GlcNAC)(2)-uptake activity, but GlcNAc transport activity was unaffected. The data indicated that msiK is essential for (GlcNAC)(2) uptake, which in S. coelicolor A3(2) is governed by ABC transporters including the DasABC-MsiK system, in contrast to Escherichia coli and Serratia marcescens, in which (GlcNAC)(2) uptake is mediated by the phosphotransferase system. Interestingly, the induction of chitinase production by (GlcNAC)(2) or chitin was absent in the msiK null mutant, unlike in the parent strain M145. The defect in chitinase gene induction was rescued by expressing the His-tagged MsiK protein under the control of the putative native promoter on a multicopy plasmid. The data suggest that uptake of (GlcNAC)(2) is necessary for induction of chitinase production. The msiK gene was constitutively transcribed, whereas the transcription of dasA [(GlcNAC)(2)-binding protein gene], malE (putative maltose-binding protein gene), cebE1 (putative cellobiose-binding protein gene) and bxlE1 (putative xylobiose-binding protein gene) was induced by their corresponding sugar ligands. This is believed to be the first report to indicate that (GlcNAC)(2) uptake mediated by ABC transporters is essential for chitinase production in streptomycetes, which are known to be the main degraders of chitin in soil.

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  • CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis 査読

    Ayako Miya, Premkumar Albert, Tomonori Shinya, Yoshitake Desaki, Kazuya Ichimura, Ken Shirasu, Yoshihiro Narusaka, Naoto Kawakami, Hanae Kaku, Naoto Shibuya

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   104 ( 49 )   19613 - 19618   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Chitin is a major component of fungal cell walls and serves as a microbe-associated molecular pattern (MAMP) for the detection of various potential pathogens in innate immune systems of both plants and animals. We recently showed that chitin elicitor-binding protein (CEBiP), plasma membrane glycoprotein with LysM motifs, functions as a cell surface receptor for chitin elicitor in rice. The predicted structure of CEBiP does not contain any intracellular domains, suggesting that an additional component(s) is required for signaling through the plasma membrane into the cytoplasm. Here, we identified a receptor-like kinase, designated CERK1, which is essential for chitin elicitor signaling in Arabidopsis. The KO mutants for CERK1 completely lost the ability to respond to the chitin elicitor, including MAPK activation, reactive oxygen species generation, and gene expression. Disease resistance of the KO mutant against an incompatible fungus, Alternaria brassicicola, was partly impaired. Complementation with the WT CERK1 gene showed cerk1 mutations were responsible for the mutant phenotypes. CERK1 is a plasma membrane protein containing three LysM motifs in the extracellular domain and an intracellular Ser/Thr kinase domain with autophosphorylation/myelin basic protein kinase activity, suggesting that CERK1 plays a critical role in fungal MAMP perception in plants.

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  • Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism 査読

    Tomonori Shinya, Ivan Galis, Tomoko Narisawa, Mami Sasaki, Hiroo Fukuda, Hideaki Matsuoka, Mikako Saito, Ken Matsuoka

    PLANT AND CELL PHYSIOLOGY   48 ( 10 )   1404 - 1413   2007年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    We previously demonstrated that a -1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

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  • The dasABC gene cluster, adjacent to dasR, encodes a novel ABC transporter for the uptake of N,N’-diacetylchitobiose in Streptomyces coelicolor A3(2).

    SAITO Akihiro, SHINYA Tomonori, MIYAMOTO Katsushiro, YOKOYAMA Tomofumi, KAKU Hanae, MINAMI Eiichi, SHIBUYA Naoto, TSUJIBO Hiroshi, NAGATA Yoshiho, ANDO Akikazu, FUJII Takeshi, MIYASHITA Kiyotaka

    Applied and Environmental Microbiology   73 ( 9 )   3000 - 3008   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1128/AEM.02612-06

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  • Characterization of NtChitIV, a class IV chitinase induced by beta-1,3-, 1,6-glucan elicitor from Alternaria alternata 102: Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV 査読

    Tomonori Shinya, Kazunari Hanai, Ivan Galis, Kaoru Suzuki, Ken Matsuoka, Hideaki Matsuoka, Mikako Saito

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   353 ( 2 )   311 - 317   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the beta-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5'-flanking region of a gene encoding class IV chitinases were isolated on the basis of the amino acid sequence of TBC-1. Sequence analysis indicated that NtChitIV encoded TBC-1, TBC-3, or both. Since purified TBC-1 and TBC-3 from BY-2 cells lack a chitin binding domain in the N-terminal region, these enzymes suggested to be derived from NtChitIV by post-translational proteolytic processing. The transcripts of NtChitIV accumulated rapidly within I h after treatment with AaGlucan. Accumulation was maximal 3 h after treatment. Reporter gene assays were used to analyze the promoter regions involved in the transcriptional control of NtChitIV, and these assays revealed that the 1.89-kb NtChitIV promoter was activated by AaGlucan but not by salicylic acid (SA) or methyl jasmonate (MeJA). The AaGlucan-induced transcriptional activation via 1.89-kb NtChitIV promoter was attenuated by pretreatment with SA or MeJA. These results suggest that NtChitIV expression is particularly induced by AaGlucan and that the AaGlucan-dependent signaling pathway is different from the SA- and MeJA-dependent signaling pathways. (c) 2006 Elsevier Inc. All rights reserved.

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  • Novel β-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco 査読

    Tomonori Shinya, Rozenn Ménard, Ikuko Kozone, Hideaki Matsuoka, Naoto Shibuya, Serge Kauffmann, Ken Matsuoka, Mikako Saito

    FEBS Journal   273 ( 11 )   2421 - 2431   2006年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A novel elicitor that induces chitinases in tobacco BY-2 cells was isolated from Alternaria alternata 102. Six other fungi, including A. alternata IFO 6587, could not induce, or weakly induce chitinase activity. The purified elicitor was soluble in 75% methanol and showed the chitinase-inducing activity when applied at concentrations of as low as 25 ng.mL-1. Structural determination by methylation analysis, reducing-end analysis, MALDI-TOF/MS, and NMR spectroscopy indicated that the elicitor was a mixture of β-1,3-, 1,6-oligoglucans mostly with a degree of polymerization of between 8 and 17. Periodate oxidation of the elicitor suggested that the 1,6-linked and nonreducing terminal residues are essential for the elicitor activity. Further analysis of the elicitor responses in BY-2 cells indicated that the activity of this β-1,3-, 1,6-glucan elicitor was about 1000 times more potent than that of laminarin, which is a known elicitor of defense responses in tobacco. Analyzing the expression of defense-related genes indicated that a phenylalanine ammonia-lyase gene and a coumaroyl-CoA O-methyltransferase gene were transiently expressed by this β-1,3-, 1,6-glucan elicitor. The elicitor induced a weak oxidative burst but did not induce cell death in the BY-2 cells. In the tissue of tobacco plants, this β-1,3-, 1,6-glucan elicitor induced the expression of basic PR-3 genes, the phenylpropanoid pathway genes, and the sesquiterpenoid pathway genes. In comparison with laminarin and laminarin sulfate, which are reported to be potent elicitors of defense responses in tobacco, the expression pattern of genes induced by the purified β-1,3-, 1,6-glucan elicitor was more similar to that induced by laminarin than to that induced by laminarin sulfate. © 2006 The Authors.

    DOI: 10.1111/j.1742-4658.2006.05249.x

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  • A novel chitinase isozyme in tobacco BY-2 cells induced by the autoclaved Alternaria alternata culture medium 査読

    Mikako Saito, Tomonori Shinya, Kazunari Hanai, Tetsuya Katagi, Hideaki Matsuoka

    Plant Science   167 ( 4 )   811 - 817   2004年10月

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    掲載種別:研究論文(学術雑誌)  

    BY-2 cells form a linear linkage of several cells. Such a unique configuration is thought to be suitable for the cell-to-cell communication analysis in vivo that is the basis of the elucidation of the systemic acquired resistance in plants. Since chitinase is one of the typical stress response proteins in plants, BY-2 cells were treated with various biotic and abiotic stresses to investigate if chitinase could be induced in BY-2 cells. Among 33 stresses, the autoclaved Alternaria alternata culture medium could induce at least three chitinase isozymes. The amount of each isozyme was very small but the most abundant one, TBC-1, could be successfully isolated at the yield of 2μg from 2.4kg wet matter of BY-2 cells. The N-terminal amino acid sequence of TBC-1 was analyzed, and 24 residues were determined. The sequence was homologous to those of class IV or II chitinases of other plants, but not homologous to those of already known tobacco chitinases. Therefore, TBC-1 was a novel isozyme of tobacco chitinase and the first chitinase found in a BY-2 cell line. © 2004 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.plantsci.2004.05.018

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  • Cell-lytic activity of tobacco BY-2 induced by a fungal elicitor from alternaria alternata attributed to the expression of a class I beta-1,3-glucanase gene. 査読 国際誌

    Tomonori Shinya, Shinobu Gondo, Hiroshi Iijima, Kazunari Hanai, Hideaki Matsuoka, Mikako Saito

    Bioscience, biotechnology, and biochemistry   68 ( 6 )   1265 - 72   2004年6月

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    記述言語:英語  

    Stress-induced cell-lytic activity was found in tobacco BY-2 cells treated with various stresses. Among 14 stresses, an elicitor fraction isolated from Alternaria alternata showed the highest inducing activity. Cell-lytic activity increased for 72 h even in the control sample, treated with distilled water, and several isozymes of beta-1,3-glucanases and chitinases were found to be involved in it. In contrast, cell-lytic activity in BY-2 cells treated with a fungal elicitor reached a higher level after 60 h. The principal enzymes specifically involved in this stress-induced portion are speculated to be basic beta-1,3-glucanases. A class I beta-1,3-glucanase gene (glu1) was found to be the specific gene for the stress-induced cell-lytic activity. Its expression became observable at 24 h, and the intensity reached a maximum at about 60-72 h. The glu1 was thus assigned as a late gene. Its role in the stress response is discussed in conjunction with earlier genes such as chitinases.

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  • Isolation of a novel isozyme of tobacco BY-2 chitinase induced by a fungal elicitor 査読

    Tomonori Shinya, Kazunari Hanai, Mikako Saito, Hideaki Matsuoka

    Plant Biotechnology   21 ( 2 )   155 - 158   2004年6月

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    掲載種別:研究論文(学術雑誌)  

    Three isozymes of chitinase were induced by a fungal elicitor in cultured tobacco BY-2 cells. The most acidic one, designated TBC-3, was described here. The molecular mass of TBC-3 was estimated to be 28.5 kDa. The N-terminal amino acid sequence of TBC-3 was analyzed and a homology search was performed. Fifteen amino acids at the N-terminus showed 60% homology to class II chitinases from other plants but not higher than 27% homology to already known chitinases from tobacco. Therefore, TBC-3 is thought to be a novel tobacco chitinase that may be classified into class II.

    DOI: 10.5511/plantbiotechnology.21.155

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MISC

  • 親和性標識実験

    新屋友規

    植物細胞壁実験法 (石井忠ら編)   374 - 376   2016年2月

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    担当区分:筆頭著者, 責任著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • Oligosaccharin receptors in plant immunity

    Tomonori Shinya, Yoshitake Desaki, Naoto Shibuya

    Research Progress in Oligosaccharins   29 - 39   2016年1月

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    © Springer Science+Business Media New York 2016. It has been well known that oligosaccharides generated from fungal, bacterial, or plant cell walls can induce various defense responses in plant cells. Traditionally they have been called as “elicitors” or “general elicitors” that induce defense responses in a wide range of plant species. These molecules are now classifi ed as representative MAMPs/PAMPs (microbe/pathogen-associated molecular patterns) or DAMPs (damage/danger-associated molecular patterns) and believed to play important roles in plant immunity, as a trigger of the so-called pattern- triggered immunity (PTI). Biological activities of these oligosaccharide elicitors, including structure/function relationships, corresponding receptors, and downstream signaling, have been the subject of intense studies, by which these oligosaccharides became most well-characterized oligosaccharins. In this chapter, present knowledge on the plant receptors of carbohydrate elicitors generated from the cell walls of pathogenic microbes and also host plants is summarized. Mechanism of the activation of downstream signaling is discussed for chitin receptor, as this system has become the most well-characterized oligosaccharin receptor in plant immunity. It is also discussed how pathogenic microbes try to escape from the MAMP- mediated detection of their invasion by host plants.

    DOI: 10.1007/978-1-4939-3518-5_3

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  • 植物免疫と細胞壁

    新屋友規, 渋谷直人

    植物の生長調節   50   76 - 82   2015年

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  • キチン・キトサンの生物活性と利用

    新屋友規, 渋谷直人

    菌類の事典 人間社会編   532 - 533   2013年

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • LysM受容体を介した植物免疫応答

    賀来華江, 新屋友規, 渋谷直人

    化学と生物   2012年

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  • 免疫装置としてのはたらき「植物の病原菌認識とシグナル伝達における細胞壁の役割」

    新屋友規, 渋谷直人

    生物の科学 遺伝   2012年

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  • キチン受容体をめぐる植物と微生物のせめぎあい

    新屋友規, 渋谷直人

    化学と生物   2010年

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担当授業科目

  • 植物免疫学特論 (2021年度) 後期  - その他

  • 植物生理学2 (2021年度) 第4学期  - 金1,金2

  • 植物遺伝学および生物ストレス学 (2021年度) 後期  - 火1~4

  • 植物-昆虫相互作用学演習 (2021年度) 前期  - その他

  • 植物-昆虫相互作用学演習 (2021年度) 後期  - その他

  • 植物-昆虫相互作用学演習 (2021年度) 後期  - その他

  • 植物-昆虫相互作用学演習 (2021年度) 前期  - その他

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 資源植物学ラボマニュアル (2021年度) 前期  - 火5-8

  • 植物免疫学特論 (2020年度) 特別  - その他

  • 植物生理学2 (2020年度) 第4学期  - 金1,金2

  • 植物遺伝学および生物ストレス学 (2020年度) 後期  - その他

  • 植物-昆虫相互作用学演習 (2020年度) 前期  - その他

  • 植物-昆虫相互作用学演習 (2020年度) 後期  - その他

  • 植物-昆虫相互作用学演習 (2020年度) 後期  - その他

  • 植物-昆虫相互作用学演習 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

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