Updated on 2025/07/17

写真a

 
SHINYA Tomonori
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
External link

Degree

  • 博士(工学) ( 東京農工大学 )

Research Interests

  • 生物間相互作用

  • Plant-insect interactions

  • Plant immunity

  • 分子間相互作用

  • 植食性昆虫

  • 害虫

  • イネ

Research Areas

  • Life Science / Applied molecular and cellular biology

  • Environmental Science/Agriculture Science / Plant protection science

  • Environmental Science/Agriculture Science / Environmental agriculture

Education

  • Tokyo University of Agriculture and Technology   工学研究科   生命工学専攻

    1999 - 2004

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    Country: Japan

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  • Tokyo University of Agriculture and Technology    

    1995 - 1999

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Research History

  • Okayama University   資源植物科学研究所   Associate Professor

    2019

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  • Okayama University   資源植物科学研究所   Assistant Professor

    2013 - 2018

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Papers

  • Natural variation-based genetic screen in rice identifies the isopentylamine biosynthetic gene that modulates brown planthopper behaviour. Reviewed International journal

    T Aboshi, M Teraishi, I Galis, T Yoshikawa, T Shinya

    Plant biology (Stuttgart, Germany)   27 ( 5 )   873 - 882   2025.8

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    We previously identified isopentylamine as a novel defence metabolite accumulated in rice (Oryza sativa L.) infested by brown planthoppers (BPHs, Nilaparvata lugens) and caterpillars, however the key biosynthetic gene(s) remained unknown. An analytical screen of genetically diverse rice cultivars was used to identify natural variation in isopentylamine production in rice, revealing that it can be produced at high levels in cv. Nipponbare but not in cv. Kasalath. Genetic approaches with chromosome fragment substitution lines and backcross inbred lines of Nipponbare and Kasalath were then used to identify genes potentially involved in biosynthesis of isopentylamine. The main candidate biosynthetic gene was knocked out with CRISPR/Cas9 to obtain three independent Nipponbare mutant lines, and correlations between gene function and isopentylamine were examined analytically. The gene Os10g0400500 was identified as the main biosynthetic gene for production of isopentylamine in rice. Furthermore, behavioural responses to isopentylamine in two-choice host assays were examined by placing BPHs in containers with mutant and wild-type Nipponbare leaves. Although BPHs were not always attracted to either wild-type or mutants, more BPHs, at least at some points, were found on the mutant leaves. Our results suggest that BPHs may show a preference for isopentylamine-deprived plants relative to wild types, which further corroborates the potential role of isopentylamine as defence against insect herbivores in chemically diverse rice species.

    DOI: 10.1111/plb.70041

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  • Spider mite tetranins elicit different defense responses in different host habitats. Reviewed International journal

    Yukiko Endo, Miku Tanaka, Takuya Uemura, Kaori Tanimura, Yoshitake Desaki, Rika Ozawa, Sara Bonzano, Massimo E Maffei, Tomonori Shinya, Ivan Galis, Gen-Ichiro Arimura

    The Plant journal : for cell and molecular biology   121 ( 5 )   e70046   2025.3

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    Spider mites (Tetranychus urticae) are a major threat to economically important crops. Here, we investigated the potential of tetranins, in particular Tet3 and Tet4, as T. urticae protein-type elicitors that stimulate plant defense. Truncated Tet3 and Tet4 proteins showed efficacy in activating the defense gene pathogenesis-related 1 (PR1) and inducing phytohormone production in leaves of Phaseolus vulgaris. In particular, Tet3 caused a drastically higher Ca2+ influx in leaves, but a lower reactive oxygen species (ROS) generation compared to other tetranins, whereas Tet4 caused a low Ca2+ influx and a high ROS generation in the host plants. Such specific and non-specific elicitor activities were examined by knockdown of Tet3 and Tet4 expressions in mites, confirming their respective activities and in particular showing that they function additively or synergistically to induce defense responses. Of great interest is the fact that Tet3 and Tet4 expression levels were higher in mites on their preferred host, P. vulgaris, compared to the levels in mites on the less-preferred host, Cucumis sativus, whereas Tet1 and Tet2 were constitutively expressed regardless of their host. Furthermore, mites that had been hosted on C. sativus induced lower levels of PR1 expression, Ca2+ influx and ROS generation, i.e., Tet3- and Tet4-responsive defense responses, in both P. vulgaris and C. sativus leaves compared to the levels induced by mites that had been hosted on P. vulgaris. Taken together, these findings show that selected tetranins respond to variable host cues that may optimize herbivore fitness by altering the anti-mite response of the host plant.

    DOI: 10.1111/tpj.70046

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  • Flower jasmonates control fertility but largely disconnect from defense metabolites in reproductive tissues of rice (Oryza sativa L.) Reviewed

    Kaori Fukumoto, Yuko Hojo, Hiroko Nakatani, David Wari, Tomonori Shinya, Ivan Galis

    Journal of Experimental Botany   2025.2

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    DOI: 10.1093/jxb/eraf073

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  • Chitin-signaling-dependent responses to insect oral secretions in rice cells propose the involvement of chitooligosaccharides in plant defense against herbivores Reviewed

    Yasukazu Kanda, Tomonori Shinya, David Wari, Yuko Hojo, Yuka Fujiwara, Wataru Tsuchiya, Zui Fujimoto, Bart P.H.J. Thomma, Yoko Nishizawa, Takashi Kamakura, Ivan Galis, Masaki Mori

    Plant Journal   121 ( 1 )   e17157   2025.1

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.17157

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  • Shoot-Silicon-Signal protein to regulate root silicon uptake in rice Reviewed

    Naoki Yamaji, Namiki Mitani-Ueno, Toshiki Fujii, Tomonori Shinya, Ji Feng Shao, Shota Watanuki, Yasunori Saitoh, Jian Feng Ma

    Nature Communications   15 ( 1 )   2024.12

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1038/s41467-024-55322-7

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    Other Link: https://www.nature.com/articles/s41467-024-55322-7

  • Comprehensive analysis of silicon impact on defense and metabolic responses in rice exposed to herbivory stress. Reviewed International journal

    Dandy Ahamefula Osibe, Yuko Hojo, Tomonori Shinya, Namiki Mitani-Ueno, Ivan Galis

    Frontiers in plant science   15   1399562 - 1399562   2024.5

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    Silicon (Si) uptake is generally beneficial for plants that need protection from insect herbivores. In pursue of mechanisms involved in Si-mediated defense, we comprehensively explored the impact of Si on several defensive and metabolic traits in rice exposed to simulated and real herbivory of Mythimna loreyi Duponchel larvae. Hydroponic experiments showed that Si-deprived rice supplemented with Si 72 h prior to insect infestation were similarly resistant to larvae as plants continuously grown in Si-containing media. Both Si and herbivory altered primary metabolism in rice, including the levels of several sugars, amino acids, and organic acids. While the accumulation of sugars was generally positively correlated with Si presence, multiple amino acids showed a negative correlation trend with Si supplementation. The levels of secondary metabolites, including isopentylamine, p-coumaroylputrescine and feruloylputrescine, were typically higher in the leaves of Si-supplemented plants exposed to herbivory stress compared to Si-deprived plants. In addition, simulated herbivory treatment in Si-supplemented plants induced more volatile emissions relative to Si-deprived plants, which was consistent with the increased transcripts of key genes involved in volatile biosynthesis. In ecological interactions, Si alone did not affect the oviposition choice of M. loreyi but gravid females showed a significant preference for simulated herbivory-treated/Si-deprived compared to Si-supplemented plants. Our data suggest that apart from mechanical defense, Si may affect rice metabolism in multiple ways that might enhance/modulate defense responses of rice under herbivory stress.

    DOI: 10.3389/fpls.2024.1399562

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  • Glucosylation prevents autotoxicity of stress inducible DOPA in maize seedlings Reviewed

    Takako Aboshi, Kohei Ittou, Ivan Galis, Tomonori Shinya, Tetsuya Murayama

    Plant Growth Regulation   101 ( 1 )   159 - 167   2023.9

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    DOI: 10.1007/s10725-023-01009-w

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  • Comparative analysis of sorghum (C4) and rice (C3) plant headspace volatiles induced by artificial herbivory Reviewed

    Cyprian Osinde, Islam S. Sobhy, David Wari, Son Truong Dinh, Yuko Hojo, Dandy A. Osibe, Tomonori Shinya, Arthur K. Tugume, Anthony M. Nsubuga, Ivan Galis

    Plant Signaling Behavior   2023.8

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    Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1080/15592324.2023.2243064

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  • BSR1, a Rice Receptor-like Cytoplasmic Kinase, Positively Regulates Defense Responses to Herbivory. Reviewed International journal

    Yasukazu Kanda, Tomonori Shinya, Satoru Maeda, Kadis Mujiono, Yuko Hojo, Keisuke Tomita, Kazunori Okada, Takashi Kamakura, Ivan Galis, Masaki Mori

    International journal of molecular sciences   24 ( 12 )   2023.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Crops experience herbivory by arthropods and microbial infections. In the interaction between plants and chewing herbivores, lepidopteran larval oral secretions (OS) and plant-derived damage-associated molecular patterns (DAMPs) trigger plant defense responses. However, the mechanisms underlying anti-herbivore defense, especially in monocots, have not been elucidated. The receptor-like cytoplasmic kinase Broad-Spectrum Resistance 1 (BSR1) of Oryza sativa L. (rice) mediates cytoplasmic defense signaling in response to microbial pathogens and enhances disease resistance when overexpressed. Here, we investigated whether BSR1 contributes to anti-herbivore defense responses. BSR1 knockout suppressed rice responses triggered by OS from the chewing herbivore Mythimna loreyi Duponchel (Lepidoptera: Noctuidae) and peptidic DAMPs OsPeps, including the activation of genes required for biosynthesis of diterpenoid phytoalexins (DPs). BSR1-overexpressing rice plants exhibited hyperactivation of DP accumulation and ethylene signaling after treatment with simulated herbivory and acquired enhanced resistance to larval feeding. As the biological significance of herbivory-induced accumulation of rice DPs remains unexplained, their physiological activities in M. loreyi were analyzed. The addition of momilactone B, a rice DP, to the artificial diet suppressed the growth of M. loreyi larvae. Altogether, this study revealed that BSR1 and herbivory-induced rice DPs are involved in the defense against chewing insects, in addition to pathogens.

    DOI: 10.3390/ijms241210395

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  • The rice wound-inducible transcription factor RERJ1 sharing same signal transduction pathway with OsMYC2 is necessary for defense response to herbivory and bacterial blight. Reviewed International journal

    Ioana Valea, Atsushi Motegi, Naoko Kawamura, Koichi Kawamoto, Akio Miyao, Rika Ozawa, Junji Takabayashi, Kenji Gomi, Keiichirou Nemoto, Akira Nozawa, Tatsuya Sawasaki, Tomonori Shinya, Ivan Galis, Koji Miyamoto, Hideaki Nojiri, Kazunori Okada

    Plant Molecular Biology   109 ( 4-5 )   651 - 666   2022.7

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    KEY MESSAGE: This study describes biological functions of the bHLH transcription factor RERJ1 involved in the jasmonate response and the related defense-associated metabolic pathways in rice, with particular focus on deciphering the regulatory mechanisms underlying stress-induced volatile emission and herbivory resistance. RERJ1 is rapidly and drastically induced by wounding and jasmonate treatment but its biological function remains unknown as yet. Here we provide evidence of the biological function of RERJ1 in plant defense, specifically in response to herbivory and pathogen attack, and offer insights into the RERJ1-mediated regulation of metabolic pathways of specialized defense compounds, such as monoterpene linalool, in possible collaboration with OsMYC2-a well-known master regulator in jasmonate signaling. In rice (Oryza sativa L.), the basic helix-loop-helix (bHLH) family transcription factor RERJ1 is induced under environmental stresses, such as wounding and drought, which are closely linked to jasmonate (JA) accumulation. Here, we investigated the biological function of RERJ1 in response to biotic stresses, such as herbivory and pathogen infection, using an RERJ1-defective mutant. Transcriptome analysis of the rerj1-Tos17 mutant revealed that RERJ1 regulated the expression of a typical family of conserved JA-responsive genes (e.g., terpene synthases, proteinase inhibitors, and jasmonate ZIM domain proteins). Upon exposure to armyworm attack, the rerj1-Tos17 mutant exhibited more severe damage than the wildtype, and significant weight gain of the larvae fed on the mutant was observed. Upon Xanthomonas oryzae infection, the rerj1-Tos17 mutant developed more severe symptoms than the wildtype. Among RERJ1-regulated terpene synthases, linalool synthase expression was markedly disrupted and linalool emission after wounding was significantly decreased in the rerj1-Tos17 mutant. RERJ1 appears to interact with OsMYC2-a master regulator of JA signaling-and many OsJAZ proteins, although no obvious epistatic interaction was detected between them at the transcriptional level. These results indicate that RERJ1 is involved in the transcriptional induction of JA-mediated stress-responsive genes via physical association with OsMYC2 and mediates defense against herbivory and bacterial infection through JA signaling.

    DOI: 10.1007/s11103-021-01186-0

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  • Chitooligosaccharide elicitor and oxylipins synergistically elevate phytoalexin production in rice Reviewed International journal

    Tomonori Shinya, Koji Miyamoto, Kenichi Uchida, Yuko Hojo, Emi Yumoto, Kazunori Okada, Hisakazu Yamane, Ivan Galis

    Plant Molecular Biology   109 ( 4-5 )   595 - 609   2022.7

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    KEY MESSAGE: We show that in rice, the amino acid-conjugates of JA precursor, OPDA, may function as a non-canonical signal for the production of phytoalexins in coordination with the innate chitin signaling. The core oxylipins, jasmonic acid (JA) and JA-Ile, are well-known as potent regulators of plant defense against necrotrophic pathogens and/or herbivores. However, recent studies also suggest that other oxylipins, including 12-oxo-phytodienoic acid (OPDA), may contribute to plant defense. Here, we used a previously characterized metabolic defense marker, p-coumaroylputrescine (CoP), and fungal elicitor, chitooligosaccharide, to specifically test defense role of various oxylipins in rice (Oryza sativa). While fungal elicitor triggered a rapid production of JA, JA-Ile, and their precursor OPDA, rice cells exogenously treated with the compounds revealed that OPDA, rather than JA-Ile, can stimulate the CoP production. Next, reverse genetic approach and oxylipin-deficient rice mutant (hebiba) were used to uncouple oxylipins from other elicitor-triggered signals. It appeared that, without oxylipins, residual elicitor signaling had only a minimal effect but, in synergy with OPDA, exerted a strong stimulatory activity towards CoP production. Furthermore, as CoP levels were compromised in the OPDA-treated Osjar1 mutant cells impaired in the oxylipin-amino acid conjugation, putative OPDA-amino acid conjugates emerged as hypothetical regulators of CoP biosynthesis. Accordingly, we found several OPDA-amino acid conjugates in rice cells treated with exogenous OPDA, and OPDA-Asp was detected, although in small amounts, in the chitooligosaccharide-treated rice. However, as synthetic OPDA-Asp and OPDA-Ile, so far, failed to induce CoP in cells, it suggests that yet another presumed OPDA-amino acid form(s) could be acting as novel regulator(s) of phytoalexins in rice.

    DOI: 10.1007/s11103-021-01217-w

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    Other Link: https://link.springer.com/article/10.1007/s11103-021-01217-w/fulltext.html

  • Integrated view of plant metabolic defense with particular focus on chewing herbivores Reviewed

    David Wari, Takako Aboshi, Tomonori Shinya, Ivan Galis

    Journal of Integrative Plant Biology   64 ( 2 )   449 - 475   2022.2

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    DOI: 10.1111/jipb.13204

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  • Phytohormone‐dependent plant defense signaling orchestrated by oral bacteria of the herbivore Spodoptera litura Reviewed

    Yukiyo Yamasaki, Hiroka Sumioka, Mayu Takiguchi, Takuya Uemura, Yuka Kihara, Tomonori Shinya, Ivan Galis, Gen‐ichiro Arimura

    New Phytologist   231 ( 5 )   2029 - 2038   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/nph.17444

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17444

  • Herbivore-induced and constitutive volatiles are controlled by different oxylipin-dependent mechanisms in rice. Reviewed International journal

    Kadis Mujiono, Tilisa Tohi, Islam S Sobhy, Yuko Hojo, Tomonori Shinya, Ivan Galis

    Plant, cell & environment   44 ( 8 )   2687 - 2699   2021.8

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    Despite the importance of volatile organic compounds (VOCs) for plants, control mechanisms for their basal and stress-induced biosynthesis and release remain unclear. We sampled and characterized headspace and internal leaf volatile pools in rice (Oryza sativa), after a simulated herbivory treatment, which triggers an endogenous jasmonate burst. Certain volatiles, such as linalool, were strongly upregulated by simulated herbivory stress. In contrast, other volatiles, such as β-caryophyllene, were constitutively emitted and fluctuated according to time of day. Transcripts of the linalool synthase gene transiently increased 1-3 h after exposure of rice to simulated herbivory, whereas transcripts of caryophyllene synthase peaked independently at dawn. Unexpectedly, although emission and accumulation patterns of rice inducible and constitutive VOCs were substantially different, both groups of volatiles were compromised in jasmonate-deficient hebiba mutants, which lack the allene oxide cyclase (AOC) gene. This suggests that rice employs at least two distinct oxylipin-dependent mechanisms downstream of AOC to control production of constitutive and herbivore-induced volatiles. Levels of the JA precursor, 12-oxo-phytodienoic acid (OPDA), were correlated with constitutive volatile levels suggesting that OPDA or its derivatives could be involved in control of volatile emission in rice.

    DOI: 10.1111/pce.14126

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  • Deciphering OPDA Signaling Components in the Momilactone-Producing Moss Calohypnum plumiforme. Reviewed International journal

    Hideo Inagaki, Koji Miyamoto, Noriko Ando, Kohei Murakami, Koki Sugisawa, Shion Morita, Emi Yumoto, Miyu Teruya, Kenichi Uchida, Nobuki Kato, Takuya Kaji, Yousuke Takaoka, Yuko Hojo, Tomonori Shinya, Ivan Galis, Akira Nozawa, Tatsuya Sawasaki, Hideaki Nojiri, Minoru Ueda, Kazunori Okada

    Frontiers in plant science   12   688565 - 688565   2021

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    Jasmonic acid (JA) and its biologically active form jasmonoyl-L-isoleucine (JA-Ile) regulate defense responses to various environmental stresses and developmental processes in plants. JA and JA-Ile are synthesized from α-linolenic acids derived from membrane lipids via 12-oxo-phytodienoic acid (OPDA). In the presence of JA-Ile, the COI1 receptor physically interacts with JAZ repressors, leading to their degradation, resulting in the transcription of JA-responsive genes by MYC transcription factors. Although the biosynthesis of JA-Ile is conserved in vascular plants, it is not recognized by COI1 in bryophytes and is not biologically active. In the liverwort Marchantia polymorpha, dinor-OPDA (dn-OPDA), a homolog of OPDA with two fewer carbons, and its isomer dn-iso-OPDA accumulate after wounding and are recognized by COI1 to activate downstream signaling. The moss Calohypnum plumiforme produces the antimicrobial-specialized metabolites, momilactones. It has been reported that JA and JA-Ile are not detected in C. plumiforme and that OPDA, but not JA, can induce momilactone accumulation and the expression of these biosynthetic genes, suggesting that OPDA or its derivative is a biologically active molecule in C. plumiforme that induces chemical defense. In the present study, we investigated the biological functions of OPDA and its derivatives in C. plumiforme. Searching for the components potentially involving oxylipin signaling from transcriptomic and genomic data revealed that two COI1, three JAZ, and two MYC genes were present. Quantification analyses revealed that OPDA and its isomer iso-OPDA accumulated in larger amounts than dn-OPDA and dn-iso-OPDA after wounding. Moreover, exogenously applied OPDA, dn-OPDA, or dn-iso-OPDA induced the transcription of JAZ genes. These results imply that OPDA, dn-OPDA, and/or their isomers potentially act as biologically active molecules to induce the signaling downstream of COI1-JAZ. Furthermore, co-immunoprecipitation analysis showed the physical interaction between JAZs and MYCs, indicating the functional conservation of JAZs in C. plumiforme with other plants. These results suggest that COI1-JAZ-MYC mediated signaling is conserved and functional in C. plumiforme.

    DOI: 10.3389/fpls.2021.688565

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  • Phytohormone elicitation in maize by oral secretions of specialist Mythimna separata and generalist Spodoptera litura Reviewed

    Morita M, Yamasaki Y, Shinya T, Galis I, Arimura GI

    J Plant Interact   16 ( 1 )   587 - 590   2021

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    Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1080/17429145.2021.2006334

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  • Ethylene functions as a suppressor of volatile production in rice. Reviewed International journal

    Kadis Mujiono, Tilisa Tohi, Islam S Sobhy, Yuko Hojo, Nhan Thanh Ho, Tomonori Shinya, Ivan Galis

    Journal of Experimental Botany   71 ( 20 )   6491 - 6511   2020.10

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    We examined the role of ethylene in the production of rice (Oryza sativa) volatile organic compounds (VOCs), which act as indirect defense signals against herbivores in tritrophic interactions. Rice plants were exposed to exogenous ethylene (1 ppm) after simulated herbivory, which consisted of mechanical wounding supplemented with oral secretions (WOS) from the generalist herbivore larva Mythimna loreyi. Ethylene treatment highly suppressed VOCs in WOS-treated rice leaves, which was further corroborated by the reduced transcript levels of major VOC biosynthesis genes in ethylene-treated rice. In contrast, the accumulation of jasmonates (JA), known to control VOCs in higher plants, and transcript levels of primary JA response genes, including OsMYC2, were not largely affected by ethylene application. At the functional level, flooding is known to promote internode elongation in young rice via ethylene signaling. Consistent with the negative role of ethylene on VOC genes, the accumulation of VOCs in water-submerged rice leaves was suppressed. Furthermore, in mature rice plants, which naturally produce less volatiles, VOCs could be rescued by the application of the ethylene perception inhibitor 1-methylcyclopropene. Our data suggest that ethylene acts as an endogenous suppressor of VOCs in rice plants during development and under stress.

    DOI: 10.1093/jxb/eraa341

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  • Silencing of phosphoinositide dependent protein kinase orthologs reduces hypersensitive cell death in Nicotiana benthamiana. Reviewed

    Akinori Kiba, Kotoko Fukui, Maki Mitani, Ivan Galis, Yuko Hojo, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Plant Biotechnology (Tokyo, Japan)   37 ( 3 )   363 - 367   2020.9

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    Phosphatidic acid plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here we focused on phosphoinositide dependent protein kinases (PDKs) as a candidate required for phosphatidic acid signaling. Based on Arabidopsis PDK sequences, we identified four putative PDK orthologs in N. benthamiana genome. To address the role of PDKs in plant defense responses, we created all four NbPDKs-silenced plants by virus-induced gene silencing. the NbPDKs-silenced plants showed a moderately reduced growth phenotype. Induction of hypersensitive cell death was compromised in the NbPDKs-silenced plants challenged with Ralstonia solanacearum. The hypersensitive cell death induced by bacterial effectors was also reduced in the NbPDKs-silenced plants. the NbPDKs-silenced plants showed decreased production of salicylic acid, jasmonic acid and jasmonoyl-L-isoleucine, as well as hydrogen peroxide after inoculation with R. solanacearum. These results suggest that NbPDKs might have an important role in the regulation of the hypersensitive cell death via plant hormone signaling and oxidative burst.

    DOI: 10.5511/plantbiotechnology.20.0511b

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  • Nonglandular silicified trichomes are essential for rice defense against chewing herbivores Reviewed

    Joackin B. Andama, Kadis Mujiono, Yuko Hojo, Tomonori Shinya, Ivan Galis

    Plant, Cell & Environment   43 ( 9 )   2019 - 2032   2020.9

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/pce.13775

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.13775

  • Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death. Reviewed International journal

    Wenxiu Ye, Shintaro Munemasa, Tomonori Shinya, Wei Wu, Tao Ma, Jiang Lu, Toshinori Kinoshita, Hanae Kaku, Naoto Shibuya, Yoshiyuki Murata

    Proceedings of the National Academy of Sciences of the United States of America   117 ( 34 )   20932 - 20942   2020.8

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    Many pathogenic fungi exploit stomata as invasion routes, causing destructive diseases of major cereal crops. Intensive interaction is expected to occur between guard cells and fungi. In the present study, we took advantage of well-conserved molecules derived from the fungal cell wall, chitin oligosaccharide (CTOS), and chitosan oligosaccharide (CSOS) to study how guard cells respond to fungal invasion. In Arabidopsis, CTOS induced stomatal closure through a signaling mediated by its receptor CERK1, Ca2+, and a major S-type anion channel, SLAC1. CSOS, which is converted from CTOS by chitin deacetylases from invading fungi, did not induce stomatal closure, suggesting that this conversion is a fungal strategy to evade stomatal closure. At higher concentrations, CSOS but not CTOS induced guard cell death in a manner dependent on Ca2+ but not CERK1. These results suggest that stomatal immunity against fungal invasion comprises not only CTOS-induced stomatal closure but also CSOS-induced guard cell death.

    DOI: 10.1073/pnas.1922319117

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  • Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana. Reviewed International journal

    Akinori Kiba, Masahito Nakano, Miki Hosokawa, Ivan Galis, Hiroko Nakatani, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Journal of Experimental Botany   71 ( 16 )   5027 - 5038   2020.8

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    Phospholipid signaling plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here, we isolated two phospholipase C2 (PLC2) orthologs in the N. benthamiana genome, designated as PLC2-1 and 2-2. Both NbPLC2-1 and NbPLC2-2 were expressed in most tissues and were induced by infiltration with bacteria and flg22. NbPLC2-1 and NbPLC2-2 (NbPLC2s) double-silenced plants showed a moderately reduced growth phenotype. The induction of the hypersensitive response was not affected, but bacterial growth and the appearance of bacterial wilt were accelerated in NbPLC2s-silenced plants when they were challenged with a virulent strain of Ralstonia solanacearum that was compatible with N. benthamiana. NbPLC2s-silenced plants showed reduced expression levels of NbPR-4, a marker gene for jasmonic acid signaling, and decreased jasmonic acid and jasmonoyl-L-isoleucine contents after inoculation with R. solanacearum. The induction of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) marker genes was reduced in NbPLC2s-silenced plants after infiltration with R. solanacearum or Pseudomonas fluorescens. Accordingly, the resistance induced by flg22 was compromised in NbPLC2s-silenced plants. In addition, the expression of flg22-induced PTI marker genes, the oxidative burst, stomatal closure, and callose deposition were all reduced in the silenced plants. Thus, NbPLC2s might have important roles in pre- and post-invasive defenses, namely in the induction of PTI.

    DOI: 10.1093/jxb/eraa233

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  • Affinity Labeling and Purification of Plant Chitin-Binding LysM Receptor with Chitin Octasaccharide Derivatives. International journal

    Tomonori Shinya, Naoto Shibuya, Hanae Kaku

    Methods in molecular biology (Clifton, N.J.)   2132   401 - 412   2020

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    Lysin motif (LysM) is a carbohydrate-binding modules found in all kingdoms. LysM binds to N-acetylglucosamine-containing molecules such as peptidoglycan, chitin, Nod factor, and Myc factor and is found in peptidoglycan hydrolases, chitinases, and plant pathogen effectors and plant receptor/co-receptor for defense and symbiosis signaling. This chapter describes the synthesis of a nonradioactive chitin ligand, biotinylated chitin octasaccharide, (GlcNAc)8-Bio, and its application for the detection and characterization of chitin-binding LysM receptor CEBiP in the microsomal membrane fraction of rice suspension-cultured cells by affinity labeling. We also describe the purification of CEBiP from the plasma membrane of the rice cells by affinity chromatography with the synthesized (GlcNAc)8-APEA-CH-Sepharose as an affinity matrix.

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  • Brown planthopper honeydew-associated symbiotic microbes elicit momilactones in rice Reviewed International journal

    David Wari, Kabir Md Alamgir, Kadis Mujiono, Yuko Hojo, Akio Tani, Tomonori Shinya, Hiroko Nakatani, Ivan Galis

    Plant Signaling & Behavior   14 ( 11 )   1655335 - 1655335   2019.11

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  • Honeydew-associated microbes elicit defense responses against brown planthopper in rice. Reviewed International journal

    David Wari, Md Alamgir Kabir, Kadis Mujiono, Yuko Hojo, Tomonori Shinya, Akio Tani, Hiroko Nakatani, Ivan Galis

    Journal of experimental botany   70 ( 5 )   1683 - 1696   2019.3

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    Feeding of sucking insects, such as the rice brown planthopper (Nilaparvata lugens; BPH), causes only limited mechanical damage on plants that is otherwise essential for injury-triggered defense responses against herbivores. In pursuit of complementary BPH elicitors perceived by plants, we examined the potential effects of BPH honeydew secretions on the BPH monocot host, rice (Oryza sativa). We found that BPH honeydew strongly elicits direct and putative indirect defenses in rice, namely accumulation of phytoalexins in the leaves, and release of volatile organic compounds from the leaves that serve to attract natural enemies of herbivores, respectively. We then examined the elicitor active components in the honeydew and found that bacteria in the secretions are responsible for the activation of plant defense. Corroborating the importance of honeydew-associated microbiota for induced plant resistance, BPHs partially devoid of their microbiota via prolonged antibiotics ingestion induced significantly less defense in rice relative to antibiotic-free insects applied to similar groups of plants. Our data suggest that rice plants may additionally perceive herbivores via their honeydew-associated microbes, allowing them to discriminate between incompatible herbivores-that do not produce honeydew-and those that are compatible and therefore dangerous.

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  • Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice. Reviewed International journal

    Tomonori Shinya, Shigetaka Yasuda, Kiwamu Hyodo, Rena Tani, Yuko Hojo, Yuka Fujiwara, Kei Hiruma, Takuma Ishizaki, Yasunari Fujita, Yusuke Saijo, Ivan Galis

    The Plant journal : for cell and molecular biology   94 ( 4 )   626 - 637   2018.5

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    Plant defense against herbivores is modulated by herbivore-associated molecular patterns (HAMPs) from oral secretions (OS) and/or saliva of insects. Furthermore, feeding wounds initiate plant self-damage responses modulated by danger-associated molecular patterns (DAMPs) such as immune defense-promoting plant elicitor peptides (Peps). While temporal and spatial co-existence of both patterns during herbivory implies a possibility of their close interaction, the molecular mechanisms remain undetermined. Here we report that exogenous application of rice (Oryza sativa) peptides (OsPeps) can elicit multiple defense responses in rice cell cultures. Specific activation of OsPROPEP3 gene transcripts in rice leaves by wounding and OS treatments further suggests a possible involvement of the OsPep3 peptide in rice-herbivore interactions. Correspondingly, we found that simultaneous application of OsPep3 and Mythimna loreyi OS significantly amplifies an array of defense responses in rice cells, including mitogen-activated protein kinase activation, and generation of defense-related hormones and metabolites. The induction of OsPROPEP3/4 by OsPep3 points to a positive auto-feedback loop in OsPep signaling which may contribute to additional enhancement of defense signal(s). Finally, the overexpression of the OsPep receptor OsPEPR1 increases the sensitivity of rice plants not only to the cognate OsPeps but also to OS signals. Our findings collectively suggest that HAMP-DAMP signal integration provides a critical step in the amplification of defense signaling in plants.

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  • Phosphoinositide 3-kinase participates in L-methionine sulfoximine-induced cell death via salicylic acid mediated signaling in Nicotiana benthamiana Reviewed

    Sayuri Sumida, Makoto Ito, Ivan Galis, Hiroko Nakatani, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba

    Journal of Plant Physiology   218   167 - 170   2017.11

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  • Oral Secretions Affect HIPVs Induced by Generalist (Mythimna loreyi) and Specialist (Parnara guttata) Herbivores in Rice. Reviewed International journal

    Islam S Sobhy, Atsushi Miyake, Tomonori Shinya, Ivan Galis

    Journal of chemical ecology   43 ( 9 )   929 - 943   2017.9

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    Plants synthesize variable mixtures of herbivore-induced plant volatiles (HIPVs) as part of their evolutionary conserved defense. To elucidate the impact of chewing herbivores with different level of adaptation on HIPV profiles in rice, we measured HIPVs released from rice seedlings challenged by either the generalist herbivore Mythimna loreyi (MYL) or the specialist Parnara guttata (PAG). Both herbivores markedly elicited the emission of HIPVs, mainly on the second and third days after attack compared to control plants. In addition, side-by-side HIPV comparisons using MYL and PAG caterpillars revealed that generalist feeding induced comparably more HIPVs relative to specialist, particularly on day two as highlighted by multivariate analysis (PLS-DA) of emitted HIPVs, and further confirmed in mimicked herbivory experiments. Here, mechanically wounded plants treated with water (WW) released more VOCs than untreated controls, and on top of this, oral secretions (OS) from both herbivores showed differential effects on volatile emissions from the wounded plants. Similar to actual herbivory, MYL OS promoted higher amounts of HIPVs relative to PAG OS, thus supporting disparate induction of rice indirect defenses in response to generalist and specialist herbivores, which could be due to the differential composition of their OS. (196 words).

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  • Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants Reviewed

    Kimiaki Tanabe, Yuko Hojo, Tomonori Shinya, Ivan Galis

    JOURNAL OF INTEGRATIVE PLANT BIOLOGY   58 ( 11 )   903 - 913   2016.11

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  • Colletotrichum higginsianum extracellular LysM proteins play dual roles in appressorial function and suppression of chitin-triggered plant immunity Reviewed

    Hiroyuki Takahara, Stephane Hacquard, Anja Kombrink, H. Bleddyn Hughes, Vivek Halder, Guillaume P. Robin, Kei Hiruma, Ulla Neumann, Tomonori Shinya, Erich Kombrink, Naoto Shibuya, Bart P. H. J. Thomma, Richard J. O'Connell

    NEW PHYTOLOGIST   211 ( 4 )   1323 - 1337   2016.9

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  • Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice Reviewed

    Tomonori Shinya, Yuko Hojo, Yoshitake Desaki, John T. Christeller, Kazunori Okada, Naoto Shibuya, Ivan Galis

    SCIENTIFIC REPORTS   6   2016.9

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    DOI: 10.1038/srep32537

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  • Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory Reviewed

    Kabir Md Alamgir, Yuko Hojo, John T. Christeller, Kaori Fukumoto, Ryutaro Isshiki, Tomonori Shinya, Ian T. Baldwin, Ivan Galis

    PLANT CELL AND ENVIRONMENT   39 ( 2 )   453 - 466   2016.2

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  • Silencing of Nicotiana benthamiana SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against Pseudomonas syringae Reviewed

    Akinori Kiba, Yu Imanaka, Masahito Nakano, Ivan Galis, Yuko Hojo, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Plant Biotechnology   33 ( 2 )   111 - 115   2016

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    DOI: 10.5511/plantbiotechnology.16.0503a

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  • Chitin-mediated plant-fungal interactions: catching, hiding and handshaking Reviewed

    Tomonori Shinya, Tomomi Nakagawa, Hanae Kaku, Naoto Shibuya

    CURRENT OPINION IN PLANT BIOLOGY   26   64 - 71   2015.8

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  • Uptake of chitosan-derived D-glucosamine oligosaccharides in Streptomyces coelicolor A3(2) Reviewed

    Pascal Viens, Marie-Pierre Dubeau, Akane Kimura, Yoshitake Desaki, Tomonori Shinya, Naoto Shibuya, Akihiro Saito, Ryszard Brzezinski

    FEMS MICROBIOLOGY LETTERS   362 ( 9 )   2015.5

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  • Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27 Reviewed

    Tomonori Shinya, Koji Yamaguchi, Yoshitake Desaki, Kenta Yamada, Tomoko Narisawa, Yoshihiro Kobayashi, Kanako Maeda, Maruya Suzuki, Takumi Tanimoto, Jun Takeda, Masato Nakashima, Ryota Funama, Mari Narusaka, Yoshihiro Narusaka, Hanae Kaku, Tsutomu Kawasaki, Naoto Shibuya

    PLANT JOURNAL   79 ( 1 )   56 - 66   2014.7

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  • Presence of LYM2 dependent but CERK1 independent disease resistance in Arabidopsis Reviewed

    Yoshihiro Narusaka, Tomonori Shinya, Mari Narusaka, Noriko Motoyama, Hikaru Shimada, Kosuke Murakami, Naoto Shibuya

    Plant Signaling and Behavior   8 ( 9 )   e25345   2013.9

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  • Functional Characterization of CEBiP and CERK1 Homologs in Arabidopsis and Rice Reveals the Presence of Different Chitin Receptor Systems in Plants Reviewed

    Tomonori Shinya, Noriko Motoyama, Asahi Ikeda, Miyuki Wada, Kota Kamiya, Masahiro Hayafune, Hanae Kaku, Naoto Shibuya

    PLANT AND CELL PHYSIOLOGY   53 ( 10 )   1696 - 1706   2012.10

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  • Effector-Mediated Suppression of Chitin-Triggered Immunity by Magnaporthe oryzae Is Necessary for Rice Blast Disease Reviewed

    Thomas A. Mentlak, Anja Kombrink, Tomonori Shinya, Lauren S. Ryder, Ippei Otomo, Hiromasa Saitoh, Ryohei Terauchi, Yoko Nishizawa, Naoto Shibuya, Bart P. H. J. Thomma, Nicholas J. Talbot

    PLANT CELL   24 ( 1 )   322 - 335   2012.1

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  • Biochemical and phylogenetic analysis of CEBiP-like LysM domain-containing extracellular proteins in higher plants. Reviewed International journal

    Judith Fliegmann, Sandra Uhlenbroich, Tomonori Shinya, Yves Martinez, Benoit Lefebvre, Naoto Shibuya, Jean-Jacques Bono

    Plant physiology and biochemistry : PPB   49 ( 7 )   709 - 20   2011.7

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    The chitin elicitor-binding protein (CEBiP) from rice was the first plant lysin motif (LysM) protein for which the biological and biochemical function had been established. It belongs to a plant-specific family of extracellular LysM proteins (LYMs) for which we analyzed the phylogeny. LYMs are present in vascular plants only, where an early gene duplication event might have resulted in two types which were retained in present day genomes. LYMs consist of a signal peptide, three consecutive LysMs, separated by cysteine pairs, and a C-terminal region without any known signature, whose length allows the distinction between the two types, and which may be followed by a glycosylphosphatidylinositol (GPI) anchor motif. We analyzed a representative of each type, MtLYM1 and MtLYM2, from Medicago truncatula at the biochemical level and with respect to their expression patterns and observed some similarities but also marked differences. MtLYM1 and MtLYM2 proved to be very different with regard to abundance and apparent molecular mass on SDS-PAGE. Both undergo several post-translational modifications, including N-glycosylation and the addition of a GPI anchor, which would position the proteins at the outer face of the plasma membrane. Only MtLYM2, but not MtLYM1, showed specific binding to biotinylated N-acetylchitooctaose in a manner similar to CEBiP, which belongs to the same type. We postulate that LYM2-type proteins likely function in the perception of chitin-related molecules, whereas possible functions of LYM1-type proteins remain to be elucidated.

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  • Conserved Fungal LysM Effector Ecp6 Prevents Chitin-Triggered Immunity in Plants Reviewed

    Ronnie de Jonge, H. Peter van Esse, Anja Kombrink, Tomonori Shinya, Yoshitake Desaki, Ralph Bours, Sander van der Krol, Naoto Shibuya, Matthieu H. A. J. Joosten, Bart P. H. J. Thomma

    SCIENCE   329 ( 5994 )   953 - 955   2010.8

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  • Glyco-conjugates as elicitors or suppressors of plant innate immunity Reviewed

    Alba Silipo, Gitte Erbs, Tomonori Shinya, J. Maxwell Dow, Michelangelo Parrilli, Rosa Lanzetta, Naoto Shibuya, Mari-Anne Newman, Antonio Molinaro

    GLYCOBIOLOGY   20 ( 4 )   406 - 419   2010.4

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  • Characterization of receptor proteins using affinity cross-linking with biotinylated ligands. Reviewed

    Tomonori Shinya, Tomohiko Osada, Yoshitake Desaki, Masahiro Hatamoto, Yuko Yamanaka, Hisashi Hirano, Ryota Takai, Fang-Sik Che, Hanae Kaku, Naoto Shibuya

    Plant & cell physiology   51 ( 2 )   262 - 70   2010.2

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    The plant genome encodes a wide range of receptor-like proteins but the function of most of these proteins is unknown. We propose the use of affinity cross-linking of biotinylated ligands for a ligand-based survey of the corresponding receptor molecules. Biotinylated ligands not only enable the analysis of receptor-ligand interactions without the use of radioactive compounds but also the isolation and identification of receptor molecules by a simple affinity trapping method. We successfully applied this method for the characterization, isolation and identification of the chitin elicitor binding protein (CEBiP). A biocytin hydrazide conjugate of N-acetylchitooctaose (GN8-Bio) was synthesized and used for the detection of CEBiP in the plasma or microsomal membrane preparations from rice and carrot cells. Binding characteristics of CEBiP analyzed by inhibition studies were in good agreement with the previous results obtained with the use of a radiolabeled ligand. The biotin-tagged CEBiP could be purified by avidin affinity chromatography and identified by LC-MALDI-MS/MS after tryptic digestion. We also used this method to detect OsFLS2, a rice receptor-like kinase for the perception of the peptide elicitor flg22, in membrane preparations from rice cells overexpressing OsFLS2. This work demonstrates the applicability of this method to the purification and identification of plant receptor proteins.

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  • Suppression of a phospholipase D gene, OsPLDbeta1, activates defense responses and increases disease resistance in rice. Reviewed International journal

    Takeshi Yamaguchi, Masaharu Kuroda, Hiromoto Yamakawa, Taketo Ashizawa, Kazuyuki Hirayae, Leona Kurimoto, Tomonori Shinya, Naoto Shibuya

    Plant physiology   150 ( 1 )   308 - 19   2009.5

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    Phospholipase D (PLD) plays an important role in plants, including responses to abiotic as well as biotic stresses. A survey of the rice (Oryza sativa) genome database indicated the presence of 17 PLD genes in the genome, among which OsPLDalpha1, OsPLDalpha5, and OsPLDbeta1 were highly expressed in most tissues studied. To examine the physiological function of PLD in rice, we made knockdown plants for each PLD isoform by introducing gene-specific RNA interference constructs. One of them, OsPLDbeta1-knockdown plants, showed the accumulation of reactive oxygen species in the absence of pathogen infection. Reverse transcription-polymerase chain reaction and DNA microarray analyses revealed that the knockdown of OsPLDbeta1 resulted in the up-/down-regulation of more than 1,400 genes, including the induction of defense-related genes such as pathogenesis-related protein genes and WRKY/ERF family transcription factor genes. Hypersensitive response-like cell death and phytoalexin production were also observed at a later phase of growth in the OsPLDbeta1-knockdown plants. These results indicated that the OsPLDbeta1-knockdown plants spontaneously activated the defense responses in the absence of pathogen infection. Furthermore, the OsPLDbeta1-knockdown plants exhibited increased resistance to the infection of major pathogens of rice, Pyricularia grisea and Xanthomonas oryzae pv oryzae. These results suggested that OsPLDbeta1 functions as a negative regulator of defense responses and disease resistance in rice.

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  • The msiK gene, encoding the ATP-hydrolysing component of N,N '-diacetylchitobiose ABC transporters, is essential for induction of chitinase production in Streptomyces coelicolor A3(2) Reviewed

    Akihiro Saito, Takeshi Fujii, Tomonori Shinya, Naoto Shibuya, Akikazu Ando, Kiyotaka Miyashita

    MICROBIOLOGY-SGM   154 ( Pt 11 )   3358 - 3365   2008.11

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    DOI: 10.1099/mic.0.2008/019612-0

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  • CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis Reviewed

    Ayako Miya, Premkumar Albert, Tomonori Shinya, Yoshitake Desaki, Kazuya Ichimura, Ken Shirasu, Yoshihiro Narusaka, Naoto Kawakami, Hanae Kaku, Naoto Shibuya

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   104 ( 49 )   19613 - 19618   2007.12

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  • Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism Reviewed

    Tomonori Shinya, Ivan Galis, Tomoko Narisawa, Mami Sasaki, Hiroo Fukuda, Hideaki Matsuoka, Mikako Saito, Ken Matsuoka

    PLANT AND CELL PHYSIOLOGY   48 ( 10 )   1404 - 1413   2007.10

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  • The dasABC gene cluster, adjacent to dasR, encodes a novel ABC transporter for the uptake of N,N '-diacetylchitobiose in Streptomyces coelicolor A3(2)

    Akihiro Saito, Tomonori Shinya, Katsushiro Miyamoto, Tomofumi Yokoyama, Hanae Kaku, Eiichi Minami, Naoto Shibuya, Hiroshi Tsujibo, Yoshiho Nagata, Akikazu Ando, Takeshi Fujii, Kiyotaka Miyashita

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   73 ( 9 )   3000 - 3008   2007.5

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  • Characterization of NtChitIV, a class IV chitinase induced by beta-1,3-, 1,6-glucan elicitor from Alternaria alternata 102: Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV Reviewed

    Tomonori Shinya, Kazunari Hanai, Ivan Galis, Kaoru Suzuki, Ken Matsuoka, Hideaki Matsuoka, Mikako Saito

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   353 ( 2 )   311 - 317   2007.2

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  • Novel beta-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco Reviewed

    T Shinya, R Menard, Kozone, I, H Matsuoka, N Shibuya, S Kauffmann, K Matsuoka, M Saito

    FEBS JOURNAL   273 ( 11 )   2421 - 2431   2006.6

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  • A novel chitinase isozyme in tobacco BY-2 cells induced by the autoclaved Alternaria alternata culture medium Reviewed

    Mikako Saito, Tomonori Shinya, Kazunari Hanai, Tetsuya Katagi, Hideaki Matsuoka

    Plant Science   167 ( 4 )   811 - 817   2004.10

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    DOI: 10.1016/j.plantsci.2004.05.018

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  • Cell-lytic activity of tobacco BY-2 induced by a fungal elicitor from alternaria alternata attributed to the expression of a class I beta-1,3-glucanase gene. Reviewed International journal

    Tomonori Shinya, Shinobu Gondo, Hiroshi Iijima, Kazunari Hanai, Hideaki Matsuoka, Mikako Saito

    Bioscience, biotechnology, and biochemistry   68 ( 6 )   1265 - 72   2004.6

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    Stress-induced cell-lytic activity was found in tobacco BY-2 cells treated with various stresses. Among 14 stresses, an elicitor fraction isolated from Alternaria alternata showed the highest inducing activity. Cell-lytic activity increased for 72 h even in the control sample, treated with distilled water, and several isozymes of beta-1,3-glucanases and chitinases were found to be involved in it. In contrast, cell-lytic activity in BY-2 cells treated with a fungal elicitor reached a higher level after 60 h. The principal enzymes specifically involved in this stress-induced portion are speculated to be basic beta-1,3-glucanases. A class I beta-1,3-glucanase gene (glu1) was found to be the specific gene for the stress-induced cell-lytic activity. Its expression became observable at 24 h, and the intensity reached a maximum at about 60-72 h. The glu1 was thus assigned as a late gene. Its role in the stress response is discussed in conjunction with earlier genes such as chitinases.

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  • Isolation of a novel isozyme of tobacco BY-2 chitinase induced by a fungal elicitor Reviewed

    Tomonori Shinya, Kazunari Hanai, Mikako Saito, Hideaki Matsuoka

    Plant Biotechnology   21 ( 2 )   155 - 158   2004.6

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    DOI: 10.5511/plantbiotechnology.21.155

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MISC

  • Sensing of herbivores by damaged self in plants Invited Reviewed

    Tomonori Shinya, Toshihisa Kotake, Ivan Galis

    Japanese Journal of Pesticide Science   47 ( 2 )   74 - 77   2022.8

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    DOI: 10.1584/jpestics.w22-17

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  • 親和性標識実験

    新屋友規

    植物細胞壁実験法 (石井忠ら編)   374 - 376   2016.2

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  • Oligosaccharin receptors in plant immunity

    Tomonori Shinya, Yoshitake Desaki, Naoto Shibuya

    Research Progress in Oligosaccharins   29 - 39   2016.1

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  • Plant immunity and cell wall(<Feature Articles>The plant cell wall as an information processing system)

    Shinya Tomonori, Shibuya Naoto

    Regulation of Plant Growth & Development   50 ( 1 )   76 - 82   2015

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    <p>Plants evolved a multilayered immune system against pathogenic microbes and insect herbivores. Plant cell wall and apoplast consist an essential component of this immune system as the first line of defense, where plant cells contact with these invaders. Plant immune responses can be triggered by the recognition of conserved microbial signature called MAMP (Microbe-associated molecular pattern) at the apoplastic region. In the plant immune system, plant cell wall can function as a barrier against invasion and also a sensor for pathogen attack. Alteration of cell wall integrity and the generation of cell wall-derived fragments are both considered to induce plant immune responses. These MAMPs and damage signals (including DAMPs, Damage-associated molecular patterns) trigger the generation of anti-microbial compounds in the apoplastic region and the reinforcement of the cell wall. On the other hand, pathogenic microbes evolved effector systems to inhibit/escape from the host defense machinery and surveillance system. These battles at the molecular level between plants and microbes often take place in the apoplast. It has also been becoming clear that the plant cell walls are involved in the detection of and defense responses against insect herbivores. Thus plant cell wall and apoplast are not only important as the battle field where host plants and invading organisms compete for the detection and defense responses but also important as a sensor and barrier for these invaders.</p>

    DOI: 10.18978/jscrp.50.1_76

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  • キチン・キトサンの生物活性と利用

    新屋友規, 渋谷直人

    菌類の事典 人間社会編   532 - 533   2013

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  • 免疫装置としてのはたらき「植物の病原菌認識とシグナル伝達における細胞壁の役割」

    新屋友規, 渋谷直人

    生物の科学 遺伝   2012

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  • LysM受容体を介した植物免疫応答

    賀来華江, 新屋友規, 渋谷直人

    化学と生物   50 ( 1 )   52 - 58   2012

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    Language:Japanese   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu.50.52

    CiNii Article

    CiNii Books

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    Other Link: https://jlc.jst.go.jp/DN/JALC/00387176671?from=CiNii

  • キチン受容体をめぐる植物と微生物のせめぎあい

    新屋友規, 渋谷直人

    化学と生物   2010

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    Authorship:Lead author  

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Research Projects

  • 植食性昆虫とその腸内細菌による危険信号分子の産生と植物の受容機構

    Grant number:21K05506  2021.04 - 2025.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    新屋 友規

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    外敵との攻防において植物が自身を守るためには、外敵を適切に認識することが防御機構を効果的に駆動するうえで重要である。食害時の損傷にともない生じる植物由来の分子の一部がエリシターとして植物に認識されることが知られており、我々も植物細胞壁由来の新規糖鎖エリシターを、イネとクサシロキヨトウの相互作用系から見出していた。本研究は、細胞壁損傷における昆虫腸内細菌の関与に注目して、植物由来エリシターの食害時産生メカニズムを明らかにするとともに、植物の昆虫エリシター認識機構に迫る研究を行うことを目的としている。
    令和3年度は、腸内細菌の植物由来エリシター産生への関与の検証を行った。抗生物質カクテルを含む餌で昆虫腸内細菌数を減少させ、エリシターとなる細胞壁断片の産生に必要な細胞壁酵素が腸内細菌に由来することを確認した。さらに、腸内細菌数を減少させたのち回収した吐き戻し液の、防御応答誘導能への影響を解析した。現在、吐き戻し液中の当該糖鎖の直接解析をLC-MSを用いて試みており、検出系が構築できた場合は、腸内細菌数を減少させたのち回収した吐き戻し液において当該糖鎖が検出されるかどうかを調べる。一方で、当該糖鎖エリシターによる一連の応答解析はイネ培養細胞の実験系を用いて行われてきたが、令和3年度はイネリーフディスクアッセイ系を用いた応答解析を行っており、次年度以降も継続して応答解析を進める。また、当該糖鎖のエリシター活性に重要な構造を明らかにするために、類似構造を有する糖鎖の応答解析を行った。

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  • Sensing of herbivores by damaged-cell wall recognition in plants

    Grant number:18K05558  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Shinya Tomonori

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    Molecules in insect oral secretions, which are deposited at the feeding area on plant leaves, play an important role in the recognition of herbivores by plants. In this study, we identified a plant cell wall derived-saccharide in the oral secretion of rice armyworm (Mythimna loreyi) larvae. The saccharide triggered an array of defense responses in rice-cultured cells. Further analysis implied that the cell wall degrading enzymes involved in the saccharide release is derived from the gut microbes of M. loreyi larvae.

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  • Elucidation of molecular mechanisms for transduction of jasmonate signal to fertility in rice

    Grant number:16K08143  2016.10 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Galis Ivan, SHINYA tomonori

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    We characterized in detail hormonal contents in rice flowers, finding that high levels of JA-Ile coincide with anthesis stage in the rice monocot model. Next, we identified multiple novel targets of JA signaling in flowers by microarray analysis, some of which belong to regulatory class genes. Out of them, novel JA-controlled NAC, MADS-box and AP2 genes of yet unknown functions were depicted as potential master regulators of fertility in rice. Furthermore, we make progress in understanding of metabolic defense in rice that is controlled by JA pathway in vegetative tissues but apparently becomes uncoupled from JA signal during later reproductive stages. Overall, our research provides advances in understanding of cross-talk between growth and defense that is important for achieving optimal fitness and growth of plants in nature.

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  • Functional role of molecular pattern recognition in plant-herbivore interactions

    Grant number:15K18820  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Shinya Tomonori

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    Plants detect herbivory through the recognition of molecular patterns derived from herbivores and themselves, however little is known about the molecular interactions of such co-existing signals during insect attack. In this study, we show that rice can simultaneously recognize several molecular patterns from rice armyworm (Mythimna loreyi) larvae. These findings suggest that a concurrent perception of diverse molecular patterns in rice may be required for a more efficient activation of its defense against herbivores.

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  • Similarities and differences in chitin-mediated recognition of microbes between plant species

    Grant number:24780334  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    SHINYA TOMONORI

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    Chitin is a major structural component of fungal cell walls and its perception triggers plant immune responses. Comparative analysis of chitin receptors in two model plants, Arabidopsis and rice, indicated species-specific differences in components of the receptor complex and their molecular characteristics. We also demonstrated that receptor-like cytoplasmic kinase PBL27 is an immediate downstream component of AtCERK1 signaling pathway in Arabidopsis.

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  • Molecular interactions at plant-insect interface

    Grant number:24570026  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    GALIS Ivan, SHINYA Tomonori, TANI Akio

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    Grant amount:\5460000 ( Direct expense: \4200000 、 Indirect expense:\1260000 )

    We established a novel system to study rice-insect interactions. We showed that the accumulation of active defense hormone jasmonoyl-L-isoleucine (JA-Ile) in rice depends on the function of OsJAR1 enzyme. JA and JA-Ile accumulations in rice were controlled by the availability of the main pathway substrate, α-linolenic acid. In comparison to wounding, rice plants specifically responded to elicitors present in chewing herbivore oral secretions. We identified two novel herbivory-related phenolamide phytoalexins. Using ROS and rice phenolamides as molecular markers, we identified and partially purified a new putative insect elicitor from Mythimna loreyi oral secretions.

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