Updated on 2025/07/17

写真a

 
SHINYA Tomonori
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
External link

Degree

  • 博士(工学) ( 東京農工大学 )

Research Interests

  • 生物間相互作用

  • Plant-insect interactions

  • Plant immunity

  • 分子間相互作用

  • 植食性昆虫

  • 害虫

  • イネ

Research Areas

  • Life Science / Applied molecular and cellular biology

  • Environmental Science/Agriculture Science / Plant protection science

  • Environmental Science/Agriculture Science / Environmental agriculture

Education

  • Tokyo University of Agriculture and Technology   工学研究科   生命工学専攻

    1999 - 2004

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    Country: Japan

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  • Tokyo University of Agriculture and Technology    

    1995 - 1999

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Research History

  • Okayama University   資源植物科学研究所   Associate Professor

    2019

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  • Okayama University   資源植物科学研究所   Assistant Professor

    2013 - 2018

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Papers

  • Natural variation-based genetic screen in rice identifies the isopentylamine biosynthetic gene that modulates brown planthopper behaviour. Reviewed International journal

    T Aboshi, M Teraishi, I Galis, T Yoshikawa, T Shinya

    Plant biology (Stuttgart, Germany)   27 ( 5 )   873 - 882   2025.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    We previously identified isopentylamine as a novel defence metabolite accumulated in rice (Oryza sativa L.) infested by brown planthoppers (BPHs, Nilaparvata lugens) and caterpillars, however the key biosynthetic gene(s) remained unknown. An analytical screen of genetically diverse rice cultivars was used to identify natural variation in isopentylamine production in rice, revealing that it can be produced at high levels in cv. Nipponbare but not in cv. Kasalath. Genetic approaches with chromosome fragment substitution lines and backcross inbred lines of Nipponbare and Kasalath were then used to identify genes potentially involved in biosynthesis of isopentylamine. The main candidate biosynthetic gene was knocked out with CRISPR/Cas9 to obtain three independent Nipponbare mutant lines, and correlations between gene function and isopentylamine were examined analytically. The gene Os10g0400500 was identified as the main biosynthetic gene for production of isopentylamine in rice. Furthermore, behavioural responses to isopentylamine in two-choice host assays were examined by placing BPHs in containers with mutant and wild-type Nipponbare leaves. Although BPHs were not always attracted to either wild-type or mutants, more BPHs, at least at some points, were found on the mutant leaves. Our results suggest that BPHs may show a preference for isopentylamine-deprived plants relative to wild types, which further corroborates the potential role of isopentylamine as defence against insect herbivores in chemically diverse rice species.

    DOI: 10.1111/plb.70041

    PubMed

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  • Spider mite tetranins elicit different defense responses in different host habitats. Reviewed International journal

    Yukiko Endo, Miku Tanaka, Takuya Uemura, Kaori Tanimura, Yoshitake Desaki, Rika Ozawa, Sara Bonzano, Massimo E Maffei, Tomonori Shinya, Ivan Galis, Gen-Ichiro Arimura

    The Plant journal : for cell and molecular biology   121 ( 5 )   e70046   2025.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    Spider mites (Tetranychus urticae) are a major threat to economically important crops. Here, we investigated the potential of tetranins, in particular Tet3 and Tet4, as T. urticae protein-type elicitors that stimulate plant defense. Truncated Tet3 and Tet4 proteins showed efficacy in activating the defense gene pathogenesis-related 1 (PR1) and inducing phytohormone production in leaves of Phaseolus vulgaris. In particular, Tet3 caused a drastically higher Ca2+ influx in leaves, but a lower reactive oxygen species (ROS) generation compared to other tetranins, whereas Tet4 caused a low Ca2+ influx and a high ROS generation in the host plants. Such specific and non-specific elicitor activities were examined by knockdown of Tet3 and Tet4 expressions in mites, confirming their respective activities and in particular showing that they function additively or synergistically to induce defense responses. Of great interest is the fact that Tet3 and Tet4 expression levels were higher in mites on their preferred host, P. vulgaris, compared to the levels in mites on the less-preferred host, Cucumis sativus, whereas Tet1 and Tet2 were constitutively expressed regardless of their host. Furthermore, mites that had been hosted on C. sativus induced lower levels of PR1 expression, Ca2+ influx and ROS generation, i.e., Tet3- and Tet4-responsive defense responses, in both P. vulgaris and C. sativus leaves compared to the levels induced by mites that had been hosted on P. vulgaris. Taken together, these findings show that selected tetranins respond to variable host cues that may optimize herbivore fitness by altering the anti-mite response of the host plant.

    DOI: 10.1111/tpj.70046

    PubMed

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  • Flower jasmonates control fertility but largely disconnect from defense metabolites in reproductive tissues of rice (Oryza sativa L.) Reviewed

    Kaori Fukumoto, Yuko Hojo, Hiroko Nakatani, David Wari, Tomonori Shinya, Ivan Galis

    Journal of Experimental Botany   2025.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jxb/eraf073

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  • Chitin-signaling-dependent responses to insect oral secretions in rice cells propose the involvement of chitooligosaccharides in plant defense against herbivores Reviewed

    Yasukazu Kanda, Tomonori Shinya, David Wari, Yuko Hojo, Yuka Fujiwara, Wataru Tsuchiya, Zui Fujimoto, Bart P.H.J. Thomma, Yoko Nishizawa, Takashi Kamakura, Ivan Galis, Masaki Mori

    Plant Journal   121 ( 1 )   e17157   2025.1

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.17157

    Scopus

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  • Shoot-Silicon-Signal protein to regulate root silicon uptake in rice Reviewed

    Naoki Yamaji, Namiki Mitani-Ueno, Toshiki Fujii, Tomonori Shinya, Ji Feng Shao, Shota Watanuki, Yasunori Saitoh, Jian Feng Ma

    Nature Communications   15 ( 1 )   2024.12

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1038/s41467-024-55322-7

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    Other Link: https://www.nature.com/articles/s41467-024-55322-7

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MISC

  • Sensing of herbivores by damaged self in plants Invited Reviewed

    Tomonori Shinya, Toshihisa Kotake, Ivan Galis

    Japanese Journal of Pesticide Science   47 ( 2 )   74 - 77   2022.8

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:Pesticide Science Society of Japan  

    DOI: 10.1584/jpestics.w22-17

    CiNii Books

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  • 親和性標識実験

    新屋友規

    植物細胞壁実験法 (石井忠ら編)   374 - 376   2016.2

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

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  • Oligosaccharin receptors in plant immunity

    Tomonori Shinya, Yoshitake Desaki, Naoto Shibuya

    Research Progress in Oligosaccharins   29 - 39   2016.1

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  • Plant immunity and cell wall(<Feature Articles>The plant cell wall as an information processing system)

    Shinya Tomonori, Shibuya Naoto

    Regulation of Plant Growth & Development   50 ( 1 )   76 - 82   2015

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    Authorship:Lead author   Language:Japanese   Publisher:The Japanese Society for Chemical Regulation of Plants  

    <p>Plants evolved a multilayered immune system against pathogenic microbes and insect herbivores. Plant cell wall and apoplast consist an essential component of this immune system as the first line of defense, where plant cells contact with these invaders. Plant immune responses can be triggered by the recognition of conserved microbial signature called MAMP (Microbe-associated molecular pattern) at the apoplastic region. In the plant immune system, plant cell wall can function as a barrier against invasion and also a sensor for pathogen attack. Alteration of cell wall integrity and the generation of cell wall-derived fragments are both considered to induce plant immune responses. These MAMPs and damage signals (including DAMPs, Damage-associated molecular patterns) trigger the generation of anti-microbial compounds in the apoplastic region and the reinforcement of the cell wall. On the other hand, pathogenic microbes evolved effector systems to inhibit/escape from the host defense machinery and surveillance system. These battles at the molecular level between plants and microbes often take place in the apoplast. It has also been becoming clear that the plant cell walls are involved in the detection of and defense responses against insect herbivores. Thus plant cell wall and apoplast are not only important as the battle field where host plants and invading organisms compete for the detection and defense responses but also important as a sensor and barrier for these invaders.</p>

    DOI: 10.18978/jscrp.50.1_76

    CiNii Article

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  • キチン・キトサンの生物活性と利用

    新屋友規, 渋谷直人

    菌類の事典 人間社会編   532 - 533   2013

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

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Research Projects

  • 植食性昆虫とその腸内細菌による危険信号分子の産生と植物の受容機構

    Grant number:21K05506  2021.04 - 2025.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    新屋 友規

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    外敵との攻防において植物が自身を守るためには、外敵を適切に認識することが防御機構を効果的に駆動するうえで重要である。食害時の損傷にともない生じる植物由来の分子の一部がエリシターとして植物に認識されることが知られており、我々も植物細胞壁由来の新規糖鎖エリシターを、イネとクサシロキヨトウの相互作用系から見出していた。本研究は、細胞壁損傷における昆虫腸内細菌の関与に注目して、植物由来エリシターの食害時産生メカニズムを明らかにするとともに、植物の昆虫エリシター認識機構に迫る研究を行うことを目的としている。
    令和3年度は、腸内細菌の植物由来エリシター産生への関与の検証を行った。抗生物質カクテルを含む餌で昆虫腸内細菌数を減少させ、エリシターとなる細胞壁断片の産生に必要な細胞壁酵素が腸内細菌に由来することを確認した。さらに、腸内細菌数を減少させたのち回収した吐き戻し液の、防御応答誘導能への影響を解析した。現在、吐き戻し液中の当該糖鎖の直接解析をLC-MSを用いて試みており、検出系が構築できた場合は、腸内細菌数を減少させたのち回収した吐き戻し液において当該糖鎖が検出されるかどうかを調べる。一方で、当該糖鎖エリシターによる一連の応答解析はイネ培養細胞の実験系を用いて行われてきたが、令和3年度はイネリーフディスクアッセイ系を用いた応答解析を行っており、次年度以降も継続して応答解析を進める。また、当該糖鎖のエリシター活性に重要な構造を明らかにするために、類似構造を有する糖鎖の応答解析を行った。

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  • Sensing of herbivores by damaged-cell wall recognition in plants

    Grant number:18K05558  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Shinya Tomonori

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    Molecules in insect oral secretions, which are deposited at the feeding area on plant leaves, play an important role in the recognition of herbivores by plants. In this study, we identified a plant cell wall derived-saccharide in the oral secretion of rice armyworm (Mythimna loreyi) larvae. The saccharide triggered an array of defense responses in rice-cultured cells. Further analysis implied that the cell wall degrading enzymes involved in the saccharide release is derived from the gut microbes of M. loreyi larvae.

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  • Elucidation of molecular mechanisms for transduction of jasmonate signal to fertility in rice

    Grant number:16K08143  2016.10 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Galis Ivan, SHINYA tomonori

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    We characterized in detail hormonal contents in rice flowers, finding that high levels of JA-Ile coincide with anthesis stage in the rice monocot model. Next, we identified multiple novel targets of JA signaling in flowers by microarray analysis, some of which belong to regulatory class genes. Out of them, novel JA-controlled NAC, MADS-box and AP2 genes of yet unknown functions were depicted as potential master regulators of fertility in rice. Furthermore, we make progress in understanding of metabolic defense in rice that is controlled by JA pathway in vegetative tissues but apparently becomes uncoupled from JA signal during later reproductive stages. Overall, our research provides advances in understanding of cross-talk between growth and defense that is important for achieving optimal fitness and growth of plants in nature.

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  • Functional role of molecular pattern recognition in plant-herbivore interactions

    Grant number:15K18820  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Shinya Tomonori

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    Plants detect herbivory through the recognition of molecular patterns derived from herbivores and themselves, however little is known about the molecular interactions of such co-existing signals during insect attack. In this study, we show that rice can simultaneously recognize several molecular patterns from rice armyworm (Mythimna loreyi) larvae. These findings suggest that a concurrent perception of diverse molecular patterns in rice may be required for a more efficient activation of its defense against herbivores.

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  • Similarities and differences in chitin-mediated recognition of microbes between plant species

    Grant number:24780334  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    SHINYA TOMONORI

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    Chitin is a major structural component of fungal cell walls and its perception triggers plant immune responses. Comparative analysis of chitin receptors in two model plants, Arabidopsis and rice, indicated species-specific differences in components of the receptor complex and their molecular characteristics. We also demonstrated that receptor-like cytoplasmic kinase PBL27 is an immediate downstream component of AtCERK1 signaling pathway in Arabidopsis.

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Class subject in charge

  • Special Seminar in Plant-Insect Interactions (2024academic year) Year-round  - その他

  • Topics in Plant immunity (2024academic year) Late  - その他

  • Plant physiology 2 (2024academic year) Fourth semester  - 金1,金2

  • Plant genetics and stress science (2024academic year) Late  - 火1~4

  • Seminar in Plant-insect interactions (2024academic year) Prophase  - その他

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