2021/07/12 更新

写真a

カツハラ マキ
且原 真木
KATSUHARA Maki
所属
資源植物科学研究所 教授
職名
教授
外部リンク

学位

  • 理学博士 ( 東京大学 )

研究キーワード

  • membrane transport

  • plant physiology

  • salt tolerance

  • stress physiology

  • 植物生理学

  • aquaporin

  • ストレス生理学

  • 膜輸送

  • アクアポリン

  • 耐塩性

研究分野

  • ライフサイエンス / 植物栄養学、土壌学

  • ライフサイエンス / 植物分子、生理科学

学歴

  • 東京大学    

    - 1990年

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    国名: 日本国

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  • 東京大学   Graduate School, Division of Science  

    - 1990年

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  • 東京大学   理学部   生物学

    - 1985年

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    国名: 日本国

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  • 東京大学   Faculty of Science  

    - 1985年

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経歴

  • - 岡山大学資源植物科学研究所 教授

    2017年

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  • - Professor,Institute of Plant Science and Resources,Okayama University

    2017年

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  • 岡山大学資源植物科学研究所 准教授   Institute of Plant Science and Resources

    2003年 - 2017年

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  • Associate Professor,Institute of Plant Science and Resources,Okayama University

    2003年 - 2017年

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所属学協会

委員歴

  • 日本植物学会   編集実行委員  

    2012年 - 2016年   

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    団体区分:学協会

    日本植物学会

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  • 日本植物生理学会   代議員  

    2012年 - 2014年   

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    団体区分:学協会

    日本植物生理学会

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  • 根研究会   英文誌編集委員会委員  

    2007年   

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    団体区分:学協会

    根研究会

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書籍等出版物

  • 植物学の百科事典(共著)

    丸善  2016年 

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  • 植物栄養学第2版(共著)

    文永堂  2010年 

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  • 水とからだの事典(共著)

    朝倉書店  2008年 

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  • みずみずしい体のしくみ:水の通り道「アクアポリン」の働きと病気」

    クバプロ  2005年 

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  • 植物の膜輸送システム ポンプ・トランスポーター・チャンネル研究の新展開

    秀潤社  2003年 

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  • Molecular aspects of potassium and water transport, and their bearing under salt stress

    Plant Nutrition for sustainable food production and environment 

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  • Molecular aspects of potassium and water transport, and their bearing under salt stress

    Plant Nutrition for sustainable food production and environment 

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  • パッチクランプによるチャンネルの解析

    「植物の細胞を観る実験プロトコール」 

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▼全件表示

MISC

  • Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport

    Jiye Rhee, Tomoaki Horie, Shizuka Sasano, Yoshiki Nakahara, Maki Katsuhara

    PHYSIOLOGIA PLANTARUM   159 ( 1 )   120 - 128   2017年1月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H2O2 permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H2O2-transporting aquaporins among 15 barley and rice TIPs. Influx of H2O2 into yeast cells expressing HvPIP2;5 was determined with a florescent-dye-based assay. Indirect immunofluorescence indicated that the expression of HvPIP2;5 protein was ubiquitous in root tissues, and was also weakly observed in leaf epidermal cells and cells in the vascular bundle. Point mutated variants of HvPIP2;5 were generated by the site-directed mutagenesis. Growth assays of yeast cells expressing these mutated HvPIP2;5 proteins suggested that Ser-126 in HvPIP2;5 has a large impact on H2O2 transport with a minor influence on the HvPIP2;5-mediated water transport.

    DOI: 10.1111/ppl.12508

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  • Exogenous application of abscisic acid (ABA) increases root and cell hydraulic conductivity and abundance of some aquaporin isoforms in the ABA-deficient barley mutant Az34

    Guzel Sharipova, Dmitriy Veselov, Guzel Kudoyarova, Wieland Fricke, Ian C. Dodd, Maki Katsuhara, Takuya Furuichi, Igor Ivanov, Stanislav Veselov

    ANNALS OF BOTANY   118 ( 4 )   777 - 785   2016年10月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Background and Aims Regulation of water channel aquaporins (AQPs) provides another mechanism by which abscisic acid (ABA) may influence water flow through plants. To the best of our knowledge, no studies have addressed the changes in ABA levels, the abundance of AQPs and root cell hydraulic conductivity (Lp(Cell)) in the same tissues. Thus, we followed the mechanisms by which ABA affects root hydraulics in an ABA-deficient barley mutant Az34 and its parental line 'Steptoe'. We compared the abundance of AQPs and ABA in cells to determine spatial correlations between AQP abundance and local ABA concentrations in different root tissues. In addition, abundance of AQPs and ABA in cortex cells was related to Lp(Cell).
    Methods Root hydraulic conductivity (Lp(Root)) was measured by means of root exudation analyses and Lp(Cell) using a cell pressure probe. The abundance of ABA and AQPs in root tissues was assessed through immunohistochemical analyses. Isoform-specific antibodies raised against HvPIP2; 1, HvPIP2; 2 and HvPIP2; 5 were used.
    Key Results Immunolocalization revealed lower ABA levels in root tissues of Az34 compared with ` Steptoe'. Root hydraulic conductivity (Lp(Root)) was lower in Az34, yet the abundance of HvPIPs in root tissues was similar in the two genotypes. Root hair formation occurred closer to the tip, while the length of the root hair zone was shorter in Az34 than in ` Steptoe'. Application of external ABA to the root medium of Az34 and ` Steptoe' increased the immunostaining of root cells for ABA and for HvPIP2; 1 and HvPIP2; 2 especially in root epidermal cells and the cortical cell layer located beneath, parallel to an increase in Lp(Root) and Lp(Cell). Treatment of roots with Fenton reagent, which inhibits AQP activity, prevented the ABA-induced increase in root hydraulic conductivity.
    Conclusion Shortly after (<2 h) ABA application to the roots of ABA-deficient barley, increased tissue ABA concentrations and AQP abundance (especially the plasma-membrane localized isoforms HvPIP2; 1 and HvPIP2; 2) were spatially correlated in root epidermal cells and the cortical cell layer located beneath, in conjunction with increased Lp(Cell) of the cortical cells. In contrast, long-term ABA deficiency throughout seedling development affects root hydraulics through other mechanisms, in particular the developmental timing of the formation of root hairs closer to the root tip and the length of the root hair zone.

    DOI: 10.1093/aob/mcw117

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  • Genome-Wide Characterization of Major Intrinsic Proteins in Four Grass Plants and Their Non-Aqua Transport Selectivity Profiles with Comparative Perspective

    Abul Kalam Azad, Jahed Ahmed, Md. Asraful Alum, Md. Mahbub Hasan, Takahiro Ishikawa, Yoshihiro Sawa, Maki Katsuhara

    PLOS ONE   11 ( 6 )   e0157735   2016年6月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Major intrinsic proteins (MIPs), commonly known as aquaporins, transport not only water in plants but also other substrates of physiological significance and heavy metals. In most of the higher plants, MIPs are divided into five subfamilies (PIPs, TIPs, NIPs, SIPs and XIPs). Herein, we identified 68, 42, 38 and 28 full-length MIPs, respectively in the genomes of four monocot grass plants, specifically Panicum virgatum, Setaria italica, Sorghum bicolor and Brachypodium distachyon. Phylogenetic analysis showed that the grass plants had only four MIP subfamilies including PIPs, TIPs, NIPs and SIPs without XIPs. Based on structural analysis of the homology models and comparing the primary selectivity-related motifs [two NPA regions, aromatic/arginine (ar/R) selectivity filter and Froger's positions (FPs)] of all plant MIPs that have been experimentally proven to transport non-aqua substrates, we predicted the transport profiles of all MIPs in the four grass plants and also in eight other plants. Groups of MIP subfamilies based on ar/R selectivity filter and FPs were linked to the non-aqua transport profiles. We further deciphered the substrate selectivity profiles of the MIPs in the four grass plants and compared them with their counterparts in rice, maize, soybean, poplar, cotton, Arabidopsis thaliana, Physcomitrella patens and Selaginella moellendorffii. In addition to two NPA regions, ar/R filter and FPs, certain residues, especially in loops B and C, contribute to the functional distinctiveness of MIP groups. Expression analysis of transcripts in different organs indicated that non-aqua transport was related to expression of MIPs since most of the unexpressed MIPs were not predicted to facilitate the transport of non-aqua molecules. Among all MIPs in every plant, TIP (BdTIP1;1, SiTIP1;2, SbTIP2;1 and PvTIP1;2) had the overall highest mean expression. Our study generates significant information for understanding the diversity, evolution, non-aqua transport profiles and insight into comparative transport selectivity of plant MIPs, and provides tools for the development of transgenic plants.

    DOI: 10.1371/journal.pone.0157735

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  • OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress

    Kei Suzuki, Naoki Yamaji, Alex Costa, Eiji Okuma, Natsuko I. Kobayashi, Tatsuhiko Kashiwagi, Maki Katsuhara, Cun Wang, Keitaro Tanoi, Yoshiyuki Murata, Julian I. Schroeder, Jian Feng Ma, Tomoaki Horie

    BMC PLANT BIOLOGY   16 ( 1 )   22   2016年1月

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Na+ exclusion from leaf blades is one of the key mechanisms for glycophytes to cope with salinity stress. Certain class I transporters of the high-affinity K+ transporter (HKT) family have been demonstrated to mediate leaf blade-Na+ exclusion upon salinity stress via Na+-selective transport. Multiple HKT1 transporters are known to function in rice (Oryza sativa). However, the ion transport function of OsHKT1;4 and its contribution to the Na+ exclusion mechanism in rice remain to be elucidated.
    Results: Here, we report results of the functional characterization of the OsHKT1; 4 transporter in rice. OsHKT1; 4 mediated robust Na+ transport in Saccharomyces cerevisiae and Xenopus laevis oocytes. Electrophysiological experiments demonstrated that OsHKT1; 4 shows strong Na+ selectivity among cations tested, including Li+, Na+, K+, Rb+, Cs+, and NH4+, in oocytes. A chimeric protein, EGFP-OsHKT1;4, was found to be functional in oocytes and targeted to the plasma membrane of rice protoplasts. The level of OsHKT1; 4 transcripts was prominent in leaf sheaths throughout the growth stages. Unexpectedly however, we demonstrate here accumulation of OsHKT1;4 transcripts in the stem including internode II and peduncle in the reproductive growth stage. Moreover, phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress. However, imposition of salinity stress on the RNAi plants in the reproductive growth stage caused significant Na+ overaccumulation in aerial organs, in particular, leaf blades and sheaths. In addition, Na-22(+) tracer experiments using peduncles of RNAi and WT plants suggested xylem Na+ unloading by OsHKT1;4.
    Conclusions: Taken together, our results indicate a newly recognized function of OsHKT1;4 in Na+ exclusion in stems together with leaf sheaths, thus excluding Na+ from leaf blades of a japonica rice cultivar in the reproductive growth stage, but the contribution is low when the plants are in the vegetative growth stage.

    DOI: 10.1186/s12870-016-0709-4

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  • OsHKT2;2/1-mediated Na+ influx over K+ uptake in roots potentially increases toxic Na+ accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress

    Kei Suzuki, Alex Costa, Hideki Nakayama, Maki Katsuhara, Atsuhiko Shinmyo, Tomoaki Horie

    JOURNAL OF PLANT RESEARCH   129 ( 1 )   67 - 77   2016年1月

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    記述言語:英語   出版者・発行元:SPRINGER JAPAN KK  

    HKT transporters are Na+-permeable membrane proteins, which mediate Na+ and K+ homeostasis in K+-depleted and saline environments in plants. Class II HKT transporters, a distinct subgroup found predominantly in monocots, are known to mediate Na+-K+ co-transport in principle. Here we report features of ion transport functions of No-OsHKT2;2/1, a class II transporter identified in a salt tolerant landrace of indica rice, Nona Bokra. We profiled No-OsHKT2;2/1 expression in organs of Nona Bokra plants with or without salinity stress. Dominant accumulation of the No-OsHKT2;2/1 transcript in K+-starved roots of Nona Bokra plants largely disappeared in response to 50 mM NaCl. We found that No-OsHKT2;2/1 expressed in the high-affinity K+ uptake deficient mutant of Saccharomyces cerevisiae and Xenopus laevis oocytes shows robust K+ selectivity even in the presence of a large amount of NaCl as reported previously. However, No-OsHKT2;2/1-expressing yeast cells exhibited Na+ hypersensitive growth under various concentrations of K+ and Na+ as the cells expressing Po-OsHKT2;2, a similar class II transporter from another salt tolerant indica rice Pokkali, when compared with the growth of cells harboring empty vector or cells expressing OsHKT2;4. The OsHKT2;4 protein expressed in Xenopus oocytes showed strong K+ selectivity in the presence of 50 mM NaCl in comparison with No-OsHKT2;2/1 and Po-OsHKT2;2. Together with apparent plasma membrane-localization of No-OsHKT2;2/1, these results point to possibilities that No-OsHKT2;2/1 could mediate destructive Na+ influx over K+ uptake in Nona Bokra plants upon salinity stress, and that a predominant physiological function of No-OsHKT2;2/1 might be the acquisition of Na+ and K+ in K+-limited environments.

    DOI: 10.1007/s10265-015-0764-1

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  • Phosphorus deprivation effects on water relations of Nicotiana tabacum plant via reducing plasma membrane permeability

    M. Mahdieh, A. Mostajeran, M. Katsuhara

    RUSSIAN JOURNAL OF PLANT PHYSIOLOGY   63 ( 1 )   54 - 61   2016年1月

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    記述言語:英語   出版者・発行元:MAIK NAUKA/INTERPERIODICA/SPRINGER  

    Plants grown in phosphorus-deprived solutions often exhibit disruption of water transport due to reduction in root hydraulic conductivity (Lp (r) ). To uncover the relationship between root Lp (r) and water permeability coefficient (P (f)) of plasma membrane and the role of aquaporins, we evaluated P (f) of plasma membrane and also PIP-type aquaporin gene expression in tobacco (Nicotiana tabacum L.) plant roots after seven days P-deprivation. The results showed significant reduction in sap flow rate (J (v)) and osmotic root hydraulic conductivity (L (pr-o)) in P-deprived roots. These effects were reversed 24 h after P-resupplying. Interestingly, the P (f) of root protoplasts was 57% lower in P-deprived plants compared with P-sufficient ones. The expression of NtPIP1;1 and NtPIP2;1 aquaporins did not change significantly in P-deprived plants compared with P-sufficient ones, but the copy number of NtAQP1 increased significantly in P-deprived plants. P-deprivation did not change Lp r-o significantly in antisense NtAQP1 plants. Taken together, these findings suggest that P-deprivation may play an important role in modulation of root hydraulic conductivity by affecting P (f) in transcellular pathway of water flow across roots and aquaporins. Finally, we concluded that dominant water transport pathway under P-deprivation was transcellular one.

    DOI: 10.1134/S102144371601012X

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  • Yeast functional screen to identify genes conferring salt stress tolerance in Salicornia europaea

    Yoshiki Nakahara, Shogo Sawabe, Kenta Kainuma, Maki Katsuhara, Mineo Shibasaka, Masanori Suzuki, Kosuke Yamamoto, Suguru Oguri, Hikaru Sakamoto

    FRONTIERS IN PLANT SCIENCE   6   920   2015年10月

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    記述言語:英語   出版者・発行元:FRONTIERS MEDIA SA  

    Salinity is a critical environmental factor that adversely affects crop productivity. Halophytes have evolved various mechanisms to adapt to saline environments. Salicornia europaea L. is one of the most salt tolerant plant species. It does not have special salt-secreting structures like a salt gland or salt bladder, and is therefore a good model for studying the common mechanisms underlying plant salt tolerance. To identify candidate genes encoding key proteins in the mediation of salt tolerance in S. europaea, we performed a functional screen of a cDNA library in yeast. The library was screened for genes that allowed the yeast to grow in the presence of 1.3 M NaCl. We obtained three full-length S. europaea genes that confer salt tolerance. The genes are predicted to encode (1) a novel protein highly homologous to thaumatin-like proteins, (2) a novel coiled-coil protein of unknown function, and (3) a novel short peptide of 32 residues. Exogenous application of a synthetic peptide corresponding to the 32 residues improved salt tolerance of Arabidopsis. The approach described in this report provides a rapid assay system for large-scale screening of S. europaea genes involved in salt stress tolerance and supports the identification of genes responsible for such mechanisms. These genes may be useful candidates for improving crop salt tolerance by genetic transformation.

    DOI: 10.3389/fpls.2015.00920

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  • Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds

    Shigeko Utsugi, Mineo Shibasaka, Masahiko Maekawa, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   56 ( 9 )   1831 - 1840   2015年9月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Tonoplast intrinsic proteins (TIPs) are involved in the transport and storage of water, and control intracellular osmotic pressure by transporting material related to the water potential of cells. In the present study, we focused on HvTIP3;1 during the periods of seed development and desiccation in barley. HvTIP3;1 was specifically expressed in seeds. An immunochemical analysis showed that HvTIP3;1 strongly accumulated in the aleurone layers and outer layers of barley seeds. The water transport activities of HvTIP3;1 and HvTIP1;2, which also accumulated in seeds, were measured in the heterologous expression system of Xenopus oocytes. When they were expressed individually, HvTIP1;2 transported water, whereas HvTIP3;1 did not. However, HvTIP3;1 exhibited water transport activity when co-expressed with HvTIP1;2 in oocytes, and this activity was higher than when HvTIP1;2 was expressed alone. This is the first report to demonstrate that the water permeability of a TIP aquaporin was activated when co-expressed with another TIP. The split-yellow fluorescent protein (YFP) system in onion cells revealed that HvTIP3;1 interacted with HvTIP1;2 to form a heterotetramer in plants. These results suggest that HvTIP3;1 functions as an active water channel to regulate water movement through tissues during the periods of seed development and desiccation.

    DOI: 10.1093/pcp/pcv104

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  • Dynamic Regulation of the Root Hydraulic Conductivity of Barley Plants in Response to Salinity/Osmotic Stress

    Toshiyuki Kaneko, Tomoaki Horie, Yoshiki Nakahara, Nobuya Tsuji, Mineo Shibasaka, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   56 ( 5 )   875 - 882   2015年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Salinity stress significantly reduces the root hydraulic conductivity (Lp(r)) of several plant species including barley (Hordeum vulgare). Here we characterized changes in the Lp(r) of barley plants in response to salinity/osmotic stress in detail using a pressure chamber. Salt-tolerant and intermediate barley cultivars, K305 and Haruna-nijyo, but not a salt-sensitive cultivar, I743, exhibited characteristic time-dependent Lp(r) changes induced by 100 mM NaCl. An identical response was evoked by isotonic sorbitol, indicating that this phenomenon was triggered by osmotic imbalances. Further examination of this mechanism using barley cv. Haruna-nijyo plants in combination with the use of various inhibitors suggested that various cellular processes such as protein phosphorylation/dephosphorylation and membrane internalization appear to be involved. Interestingly, the three above-mentioned barley cultivars did not exhibit a remarkable difference in root cell sap osmolality under hypertonic conditions, in contrast to the case of Lp(r). The possible biological significance of the regulation of Lp(r) in barley plants upon salinity/osmotic stress is discussed.

    DOI: 10.1093/pcp/pcv013

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  • Osmotic stress decreases PIP aquaporin transcripts in barley roots but H2O2 is not involved in this process

    Maki Katsuhara, Nobuya Tsuji, Mineo Shibasaka, Sanjib Kumar Panda

    JOURNAL OF PLANT RESEARCH   127 ( 6 )   787 - 792   2014年11月

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    記述言語:英語   出版者・発行元:SPRINGER JAPAN KK  

    Previous reports indicate that salt stress reduces the root hydraulic conductance and the expression of plasmamembrane-type aquaporins (PIPs). As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decreases PIP transcripts. Eight of ten PIP transcripts were reduced to less than half by 360 mM mannitol treatment for 12 h in comparison with control samples. A large decrease of HvPIP2; 1 protein was also recorded. This reduction of both transcripts and proteins of HvPIP2s should be physiologically effective for preventing or reducing dehydration at an initial phase of severe salt/osmotic stress. Root cell sap osmolality increased from 278 to 372 mOsm 24 h after 360 mM mannitol treatment. These steps can secure survival and growth recovery with water reabsorption in barley. Our data also suggest that H2O2 seems not to be the main cause of osmotic stress-induced transcriptional down-regulation within the concentrations (20-500 mu M) and time periods (24 h) examined, although H2O2 was previously proposed to be involved in the mechanisms of salinity/osmotic tolerance.

    DOI: 10.1007/s10265-014-0662-y

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  • Expression of root HvPIPs of barley seedling under chilling stress

    Rui-Jun Duan, Hui-Yan Xiong, Katsuhara Maki

    Zhiwu Shengli Xuebao/Plant Physiology Journal   50 ( 8 )   1203 - 1208   2014年8月

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    記述言語:中国語   出版者・発行元:Science Press  

    The plant plasma membrane aquaporin (plasma membrane intrinsic proteins, PIPs) is a kind of membrane intrinsic proteins which has high selectivity and water transport. They play important roles in many physiological activities in plant growth and development. In this study, seedlings of barley variety 'Haruna-nijo' were treated with chilling stress. Expression of water channel protein PIPs of root in two processes which were stress period (4℃, 48 h) and recovery period (16℃, 48 h) were analyzed with quantitative real-time reverse transcriptase PCR (qRT-PCR). And root hydraulic conductivity (Lpr), root length and seedling height were analyzed at the same time. The results showed that: after 4℃ and 48 h stress, the growth of the barley seedlings was significantly inhibited, but root growth was no significant changed
    after 16℃, 48 h recovery, shoot height of stressed-seedling caught up that of control seedlings and root growth did not change significantly
    root hydraulic conductivity decreased in the period of chilling stress and increased rapidly in recovery period but there were no significant difference. The results of qRT-PCR showed: the highest expression were HvPIP1
    2 and HvPIP1
    3, the lowest expression were HvPIP1
    1 and HvPIP2
    3. Compared with the control group, HvPIPs expression were decreased in all after cold treatment, in which HvPIP1
    2, HvPIP1
    3, HvPIP1
    4, HvPIP1
    5, HvPIP2
    1, HvPIP2
    2 were down-regulated significantly. In recovery period, most of HvPIPs expression were increased, espeacally HvPIP1
    1, HvPIP1
    2, HvPIP1
    5, HvPIP2
    3 were up-regulated significantly, but HvPIP1
    4, HvPIP2
    5 were down-regulated. This study found that HvPIPs expressions of barley root were down-regulated overall in chilling stress and in recovery growth most HvPIPs were up-regulated. Combined with changes of root hydraulic conductivity and plant growth, the role of barley HvPIPs in chilling resistance reaction speculated that HvPIPs effected water absorption were different in different stages.

    DOI: 10.13592/j.cnki.ppj.2014.0242

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  • CO2 Transport by PIP2 Aquaporins of Barley

    Izumi C. Mori, Jiye Rhee, Mineo Shibasaka, Shizuka Sasano, Toshiyuki Kaneko, Tomoaki Horie, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   55 ( 2 )   251 - 257   2014年2月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    CO2 permeability of plasma membrane intrinsic protein 2 (PIP2) aquaporins of Hordeum vulgare L. was investigated. Five PIP2 members were heterologously expressed in Xenopus laevis oocytes. CO2 permeability was determined by decrease of cytosolic pH in CO2-enriched buffer using a hydrogen ion-selective microelectrode. HvPIP2; 1, HvPIP2; 2, HvPIP2; 3 and HvPIP2; 5 facilitated CO2 transport across the oocyte cell membrane. However, HvPIP2; 4 that is highly homologous to HvPIP2; 3 did not. The isoleucine residue at position 254 of HvPIP2; 3 was conserved in PIP2 aquaporins of barley, except HvPIP2; 4, which possesses methionine instead. CO2 permeability was lost by the substitution of the Ile254 of HvPIP2; 3 by methionine, while water permeability was not affected. These results suggest that PIP2 aquaporins are permeable to CO2. and the conserved isoleucine at the end of the E-loop is crucial for CO2 selectivity.

    DOI: 10.1093/pcp/pcu003

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  • Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite

    Maki Katsuhara, Shizuka Sasano, Tomoaki Horie, Tadashi Matsumoto, Jiye Rhee, Mineo Shibasaka

    PLANT BIOTECHNOLOGY   31 ( 3 )   213 - U173   2014年

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    記述言語:英語   出版者・発行元:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    Mercury-sensitive water transport activities were detected in seven NIP (Nodulin 26-like intrinsic protein) type aquaporins among eleven NIPs examined. Amino acid substitutions in rice OsNIP3;3 revealed that mercury-sensitivity depended on a histidine (but not on a cysteine) in apoplastic loop C in plant NIP aquaporins, although the cysteine is involved in the mercury-sensitivity of animal aquaporins. Rice OsNIP3;3 was also first identified as a unique aquaporin facilitating all water, hydrogen peroxide and arsenite transports. In rice OsNIP3;2, hydrogen peroxide and arsenite transport activities were detected, but water transport was not. Barley HvNIP1;2- or rice OsNIP2;1-expressing yeast cells showed the arsenite transport activity but not the H2O2 transport activity. The present work revealed novel molecular mechanisms of water and other low molecular weight compounds transport/selection in barley and rice NIP aquaporins, including the histidine-related mercury-sensitivity in the water transport of aquaporins.

    DOI: 10.5511/plantbiotechnology.14.0421a

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  • The photosynthetic response of tobacco plants overexpressing ice plant aquaporin McMIPB to a soil water deficit and high vapor pressure deficit

    Miki Kawase, Yuko T. Hanba, Maki Katsuhara

    JOURNAL OF PLANT RESEARCH   126 ( 4 )   517 - 527   2013年7月

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    記述言語:英語   出版者・発行元:SPRINGER JAPAN KK  

    We investigated the photosynthetic capacity and plant growth of tobacco plants overexpressing ice plant (Mesembryanthemum crystallinum L.) aquaporin McMIPB under (1) a well-watered growth condition, (2) a well-watered and temporal higher vapor pressure deficit (VPD) condition, and (3) a soil water deficit growth condition to investigate the effect of McMIPB on photosynthetic responses under moderate soil and atmospheric humidity and water deficit conditions. Transgenic plants showed a significantly higher photosynthesis rate (by 48 %), higher mesophyll conductance (by 52 %), and enhanced growth under the well-watered growth condition than those of control plants. Decreases in the photosynthesis rate and stomatal conductance from ambient to higher VPD were slightly higher in transgenic plants than those in control plants. When plants were grown under the soil water deficit condition, decreases in the photosynthesis rate and stomatal conductance were less significant in transgenic plants than those in control plants. McMIPB is likely to work as a CO2 transporter, as well as control the regulation of stomata to water deficits.

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  • Overexpression of Alternative Oxidase Gene Confers Aluminum Tolerance by Altering the Respiratory Capacity and the Response to Oxidative Stress in Tobacco Cells

    Sanjib Kumar Panda, Lingaraj Sahoo, Maki Katsuhara, Hideaki Matsumoto

    MOLECULAR BIOTECHNOLOGY   54 ( 2 )   551 - 563   2013年6月

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    記述言語:英語   出版者・発行元:HUMANA PRESS INC  

    Aluminum (Al) stress represses mitochondrial respiration and produces reactive oxygen species (ROS) in plants. Mitochondrial alternative oxidase (AOX) uncouples respiration from mitochondrial ATP production and may improve plant performance under Al stress by preventing excess accumulation of ROS. We tested respiratory changes and ROS production in isolated mitochondria and whole cell of tobacco (SL, ALT 301) under Al stress. Higher capacities of AOX pathways relative to cytochrome pathways were observed in both isolated mitochondria and whole cells of ALT301 under Al stress. AOX1 when studied showed higher AOX1 expression in ALT 301 than SL cells under stress. In order to study the function of tobacco AOX gene under Al stress, we produced transformed tobacco cell lines by introducing NtAOX1 expressed under the control of the cauliflower mosaic virus (CaMV) 35 S promoter in sensitive (SL) Nicotiana tabacum L. cell lines. The enhancement of endogenous AOX1 expression and AOX protein with or without Al stress was in the order of transformed tobacco cell lines > ALT301 > wild type (SL). A decreased respiratory inhibition and reduced ROS production with a better growth capability were the significant features that characterized AOX1 transformed cell lines under Al stress. These results demonstrated that AOX plays a critical role in Al stress tolerance with an enhanced respiratory capacity, reducing mitochondrial oxidative stress burden and improving the growth capability in tobacco cells.

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  • Water and CO2 permeability of SsAqpZ, the cyanobacterium Synechococcus sp. PCC7942 aquaporin

    Xiaodong Ding, Tadashi Matsumoto, Patrizia Gena, Chengwei Liu, Marialuisa Pellegrini-Calace, Shihua Zhong, Xiaoli Sun, Yanming Zhu, Maki Katsuhara, Ikuko Iwasaki, Yoshichika Kitagawa, Giuseppe Calamita

    Biology of the Cell   105 ( 3 )   118 - 128   2013年3月

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    記述言語:英語  

    Background information: Cyanobacteria possess Aquaporin-Z (AqpZ) membrane channels which have been suggested to mediate the water efflux underlying osmostress-inducible gene expression and to be essential for glucose metabolism under photomixotrophic growth. However, preliminary observations suggest that the biophy-sical properties of transport and physiological meaning of AqpZ in such photosynthetic microorganisms are not yet completely assessed. Results: In this study, we used Xenopus laevis oocyte and proteoliposome systems to directly demonstrate the water permeability of the cyanobacterium Synechococcus sp. PCC7942 aquaporin, SsAqpZ. By an in vitro assay of intracellular acidification in yeast cells, SsAqpZ was found to transport also CO2. Consistent with this result, during the entire exponential phase of growth, Synechococcus SsAqpZ-null-mutant cells grew slower than the corresponding wild-type cells. This phenotype was stronger with higher levels of extracellular CO2. In line with the conversion of CO2 gas into HCO3- ions under alkaline conditions, the impairment in growth of the SsAqpZ-null strain was weaker in more alkaline culture medium. Conclusions: Cyanobacterial SsAqpZ may exert a pleiotropic function in addition to the already reported roles in macronutrient homeostasis and osmotic-stress response as it appears to constitute an important pathway in CO2 uptake, a fundamental step in photosynthesis. © 2013 Soçiété Française des Microscopies and Soçiété de Biologie Cellulaire de France.

    DOI: 10.1111/boc.201200057

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  • Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination

    Chengwei Liu, Tatsuya Fukumoto, Tadashi Matsumoto, Patrizia Gena, Daniele Frascaria, Tomoyuki Kaneko, Maki Katsuhara, Shihua Zhong, Xiaoli Sun, Yanming Zhu, Ikuko Iwasaki, Xiaodong Ding, Giuseppe Calamita, Yoshichika Kitagawa

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   63   151 - 158   2013年2月

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    記述言語:英語   出版者・発行元:ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    OsPIP1;1 is one of the most abundant aquaporins in rice leaves and roots and is highly responsible to environmental stresses. However, its biochemical and physiological functions are still largely unknown. The oocyte assay data showed OsPIP1;1 had lower water channel activity in contrast to OsPIP2;1. EGFP and immunoelectron microscopy studies revealed OsPIP1;1 was predominantly localized in not only plasma membrane but also in some ER-like intracellular compartments in the cells. OsPIP1;1 exhibited low water channel activity in Xenopus oocytes but coexpression of OsPIP2;1 significantly enhanced its water permeability. Stop-flow assay indicated that 10His-OsPIP1;1-reconstituted proteoliposomes had significantly higher water permeability than the control liposomes. Overexpression of OsPIP1;1 greatly altered many physiological features of transgenic plants in a dosage-dependent manner. Moderate expression of OsPIP1;1 increased rice seed yield, salt resistance, root hydraulic conductivity, and seed germination rate. This work suggests OsPIP1;1 functions as an active water channel and plays important physiological roles. Published by Elsevier Masson SAS.

    DOI: 10.1016/j.plaphy.2012.11.018

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  • Influence of Low Air Humidity and Low Root Temperature on Water Uptake, Growth and Aquaporin Expression in Rice Plants

    Tsuneo Kuwagata, Junko Ishikawa-Sakurai, Hidehiro Hayashi, Kiyoshi Nagasuga, Keiko Fukushi, Arifa Ahamed, Katsuko Takasugi, Maki Katsuhara, Mari Murai-Hatano

    PLANT AND CELL PHYSIOLOGY   53 ( 8 )   1418 - 1431   2012年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    The effects of low air humidity and low root temperature (LRT) on water uptake, growth and aquaporin gene expression were investigated in rice plants. The daily transpiration of the plants grown at low humidity was 1.5- to 2-fold higher than that at high humidity. LRT at 13 degrees C reduced transpiration, and the extent was larger at lower humidity. LRT also reduced total dry matter production and leaf area expansion, and the extent was again larger at lower humidity. These observations suggest that the suppression of plant growth by LRT is associated with water stress due to decreased water uptake ability of the root. On the other hand, the net assimilation rate was not affected by low humidity and LRT, and water use efficiency was larger for LRT. We found that low humidity induced coordinated up-regulation of many PIP and TIP aquaporin genes in both the leaves and the roots. Expression levels of two root-specific aquaporin genes, OsPIP2;4 and OsPIP2;5, were increased significantly after 6 and 13 d of LRT exposure. Taken together, we discuss the possibility that aquaporins are part of an integrated response of this crop to low air humidity and LRT.

    DOI: 10.1093/pcp/pcs087

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  • Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana

    Cortwa Hooijmaijers, Ji Ye Rhee, Kyung Jin Kwak, Gap Chae Chung, Tomoaki Horie, Maki Katsuhara, Hunseung Kang

    JOURNAL OF PLANT RESEARCH   125 ( 1 )   147 - 153   2012年1月

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    記述言語:英語   出版者・発行元:SPRINGER TOKYO  

    Although aquaporins have been known to transport hydrogen peroxide (H(2)O(2)) across cell membranes, the H(2)O(2)-regulated expression patterns and the permeability of every family member of the plasma membrane intrinsic protein (PIP) toward H(2)O(2) have not been determined. This study investigates the H(2)O(2)-regulated expression levels of all plasma membrane aquaporins of Arabidopsis thaliana (AtPIPs), and determines the permeability of every AtPIP for H(2)O(2) in yeast. Hydrogen peroxide treatment of Arabidopsis down-regulated the expression of AtPIP2 subfamily in roots but not in leaves, whereas the expression of AtPIP1 subfamily was not affected by H(2)O(2) treatment. The growth and survival of yeast cells that expressed AtPIP2;2, AtPIP2;4, AtPIP2;5, or AtPIP2;7 was reduced in the presence of H(2)O(2), while the growth of yeast cells expressing any other AtPIP family member was not affected by H(2)O(2). These results show that only certain isoforms of AtPIPs whose expression is regulated by H(2)O(2) treatment are permeable for H(2)O(2) in yeast cells, and suggest that the integrated regulation of aquaporin expression by H(2)O(2) and the capacity of individual aquaporin to transport H(2)O(2) are important for plant response to H(2)O(2).

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  • Functional characterization of a novel plasma membrane intrinsic protein2 in barley

    Mineo Shibasaka, Sizuka Sasano, Sigeko Utsugi, Maki Katsuhara

    Plant Signaling and Behavior   7 ( 12 )   1642 - 1646   2012年

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    記述言語:英語  

    Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2
    8 in barley (Hordeum vulgare). HvPIP2
    8 shared 72-76% identity with other HvPIP2s and 74% identity with rice OsPIP2
    8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2
    8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2
    8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1
    2 was not observed. These results suggest that HvPIP2
    8 plays a role in water homeostasis although further functional analysis is required in future. © 2012 Landes Bioscience.

    DOI: 10.4161/psb.22294

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  • Effect of nutrient deficiencies on the water transport properties in figleaf gourd plants

    Ji Ye Rhee, Gap Chae Chung, Maki Katsuhara, Sung-Ju Ahn

    HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY   52 ( 6 )   629 - 634   2011年12月

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    記述言語:英語   出版者・発行元:KOREAN SOC HORTICULTURAL SCIENCE  

    Effects of nitrogen, phosphorus, and potassium deficiencies on water transport properties in figleaf gourd plants were studied. Plants were treated for different period of deficiency and physiological parameters such as stomatal conductance, photosynthesis and transpiration were measured. Cell and root pressure probes were utilized to measure turgor and root pressures, half-times of water exchange and hydraulic conductivities to analyze water transport properties. When plants were grown in nitrogen or phosphorus deficient nutrient solutions, they became insensitive to mercury, suggesting that aquaporin was closed resulting in reduced hydraulic conductivity. Inclusion of tungstate, however, restored the sensitivity of cells to mercury, indicating the importance of internal nutrient concentration, not the incoming nutrient supply. The hydrostatic hydraulic conductivity of roots grown in nitrogen deficient solution, representing apoplastic pathway of water transport, was reduced but this reduction was dramatically recovered by the application of tungstate, indicating the importance of nutrient availability from storage pools in relation to water status of plants.

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  • K+ Transport by the OsHKT2;4 Transporter from Rice with Atypical Na+ Transport Properties and Competition in Permeation of K+ over Mg2+ and Ca2+ Ions

    Tomoaki Horie, Dennis E. Brodsky, Alex Costa, Toshiyuki Kaneko, Fiorella Lo Schiavo, Maki Katsuhara, Julian I. Schroeder

    PLANT PHYSIOLOGY   156 ( 3 )   1493 - 1507   2011年7月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Members of class II of the HKT transporters, which have thus far only been isolated from grasses, were found to mediate Na+-K+ cotransport and at high Na+ concentrations preferred Na+-selective transport, depending on the ionic conditions. But the physiological functions of this K+-transporting class II of HKT transporters remain unknown in plants, with the exception of the unique class II Na+ transporter OsHKT2;1. The genetically tractable rice (Oryza sativa; background Nipponbare) possesses two predicted K+-transporting class II HKT transporter genes, OsHKT2;3 and OsHKT2;4. In this study, we have characterized the ion selectivity of the class II rice HKT transporter OsHKT2;4 in yeast and Xenopus laevis oocytes. OsHKT2;4 rescued the growth defect of a K+ uptake-deficient yeast mutant. Green fluorescent protein-OsHKT2;4 is targeted to the plasma membrane in transgenic plant cells. OsHKT2;4-expressing oocytes exhibited strong K+ permeability. Interestingly, however, K+ influx in OsHKT2;4-expressing oocytes did not require stimulation by extracellular Na+, in contrast to other class II HKT transporters. Furthermore, OsHKT2;4-mediated currents exhibited permeabilities to both Mg2+ and Ca2+ in the absence of competing K+ ions. Comparative analyses of Ca2+ and Mg2+ permeabilities in several HKT transporters, including Arabidopsis thaliana HKT1;1 (AtHKT1;1), Triticum aestivum HKT2;1 (TaHKT2;1), OsHKT2;1, OsHKT2;2, and OsHKT2;4, revealed that only OsHKT2;4 and to a lesser degree TaHKT2;1 mediate Mg2+ transport. Interestingly, cation competition analyses demonstrate that the selectivity of both of these class II HKT transporters for K+ is dominant over divalent cations, suggesting that Mg2+ and Ca2+ transport via OsHKT2;4 may be small and would depend on competing K+ concentrations in plants.

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  • Mechanisms of Water Transport Mediated by PIP Aquaporins and Their Regulation Via Phosphorylation Events Under Salinity Stress in Barley Roots

    Tomoaki Horie, Toshiyuki Kaneko, Genki Sugimoto, Shizuka Sasano, Sanjib Kumar Panda, Mineo Shibasaka, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   52 ( 4 )   663 - 675   2011年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Water homeostasis is crucial to the growth and survival of plants under water-related stress. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. Here we report the water transport activity and mechanisms for the regulation of barley (Hordeum vulgare) PIP aquaporins. HvPIP2 but not HvPIP1 channels were found to show robust water transport activity when expressed alone in Xenopus laevis oocytes. However, the co-expression of HvPIP1 with HvPIP2 in oocytes resulted in significant increases in activity compared with the expression of HvPIP2 alone, suggesting the participation of HvPIP1 in water transport together with HvPIP2 presumably through heteromerization. Severe salinity stress (200 mM NaCl) significantly reduced root hydraulic conductivity (Lp(r)) and the accumulation of six of 10 HvPIP mRNAs. However, under relatively mild stress (100 mM NaCl), only a moderate reduction in Lp(r) with no significant difference in HvPIP mRNA levels was observed. Sorbitol-mediated osmotic stress equivalent to 100 and 200 mM NaCl induced nearly identical Lp(r) reductions in barley roots. Furthermore, the water transport activity in intact barley roots was suggested to require phosphorylation that is sensitive to a kinase inhibitor, staurosporine. HvPIP2s also showed water efflux activity in Xenopus oocytes, suggesting a potential ability to mediate water loss from cells under hypertonic conditions. Water transport via HvPIP aquaporins and the significance of reductions of Lp(r) in barley plants during salinity stress are discussed.

    DOI: 10.1093/pcp/pcr027

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  • Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells

    Tomoaki Horie, Mitsuo Sugawara, Tomoyuki Okada, Koichiro Taira, Pulla Kaothien-Nakayama, Maki Katsuhara, Atsuhiko Shinmyo, Hideki Nakayama

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   111 ( 3 )   346 - 356   2011年3月

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    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Potassium ion (K(+)) plays vital roles in many aspects of cellular homeostasis including competing with sodium ion (Na(+)) during potassium starvation and salt stress. Therefore, one way to engineer plant cells with improved salt tolerance is to enhance K(+) uptake activity of the cells, while keeping Na(+) out during salt stress. Here, in search for Na(+)-insensitive transporter for this purpose, bacterial expression system was used to characterize two K(+) transporters, OsHAK2 and OsHAK5, isolated from rice (Oryza sativa cv. Nipponbare). The two OsHAK transporters are members of a KT/HAK/KUP transporter family, which is one of the major K(+) transporter families in bacteria, fungi and plants. When expressed in an Escherichia cola K(+) transport mutant strain LB2003, both OsHAK transporters rescued the growth defect in K(+)-limiting conditions by significantly increasing the K(+) content of the cells. Under the condition with a large amount of extracellular Na(+), we found that OsHAK5 functions as a Na(+)-insensitive K(+) transporter, while OsHAK2 is sensitive to extracellular Na(+) and exhibits higher Na(+) over K(+) transport activities. Moreover, constitutive expression of OsHAK5 in cultured-tobacco BY2 (Nicotiana tabacum cv. Bright Yellow 2) cells enhanced the accumulation of K(+) but not Na(+) in the cells during salt stress and conferred increased salt tolerance to the cells. Transient expression experiment indicated that OsHAK5 is localized to the plant plasma membrane. These results suggest that the plasma-membrane localized Na(+) insensitive K(+) transporters, similar to OsHAK5 identified here, could be used as a tool to enhance salt tolerance in plant cells. (C) 2010, The Society for Biotechnology, japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2010.10.014

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  • Abiotic stresses modulate expression of major intrinsic proteins in barley (Hordeum vulgare)

    Ayalew Ligaba, Maki Katsuhara, Mineo Shibasaka, Gemechis Djira

    COMPTES RENDUS BIOLOGIES   334 ( 2 )   127 - 139   2011年2月

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    記述言語:英語   出版者・発行元:ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    In one of the most important crops, barley (Hordeum vulgare L), gene expression and physiological roles of most major intrinsic proteins (MIPs) remained to be elucidated. Here we studied expression of five tonoplast intrinsic protein isoforms (HvTIP1;2, HvTIP2:1, HvTIP2;2,HvTIP2;3 and HvTIP4:1), a NOD26-like intrinsic protein (HvNIP2;1) and a plasma membrane intrinsic protein (HvPIP2;1) by using the quantitative real-time RT-PCR. Five-day-old seedlings were exposed to abiotic stresses (salt, heavy metals and nutrient deficiency), abscisic acid (ABA) and gibberellic acid (GA) for 24 h. Treatment with 100 mM NaCl, 0.1 mM ABA and 1 mM GA differentially regulated gene expression in roots and shoots. Nitrogen and prolonged P-deficiency downregulated expression of most MIP genes in roots. Intriguingly, gene expression was restored to the values in the control three days after nutrient supply was resumed. Heavy metals (0.2 mM each of Cd, Cu, Zn and Cr) downregulated the transcript levels by 60-80% in roots, whereas 0.2 mM Hg upregulated expressions of most genes in roots. This was accompanied by a 45% decrease in the rate of transpiration. In order to study the physiological role of the MIPs, cDNA of three genes (HvTIP2:1, HvTIP2:3 and HvNIP2;1) have been cloned and heterologous expression was performed in Xenopus laevis oocytes. Osmotic water permeability was determined by a swelling assay. However, no water uptake activity was observed for the three proteins. Hence, the possible physiological role of the proteins is discussed. (C) 2010 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.

    DOI: 10.1016/j.crvi.2010.11.005

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  • Hormonal treatment of the bark of rubber trees (Hevea brasiliensis) increases latex yield through latex dilution in relation with the differential expression of two aquaporin genes

    Kessarin Tungngoen, Unchera Viboonjun, Panida Kongsawadworakul, Maki Katsuhara, Jean-Louis Julien, Soulaiman Sakr, Herve Chrestin, Jarunya Narangajavana

    JOURNAL OF PLANT PHYSIOLOGY   168 ( 3 )   253 - 262   2011年2月

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    記述言語:英語   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Natural rubber is synthesized in laticifers in the inner liber of the rubber tree (Hevea brasiliensis). Upon bark tapping, the latex is expelled due to fiber turgor pressure. The mature laticifers are devoid of plasmodesmata; therefore a corresponding decrease-in the total latex solid content is likely to occur due to water influx inside the laticifers. Auxins and ethylene used as efficient yield stimulants in mature untapped rubber trees, but, bark treatments with abscisic acid (ABA) and salicylic acid (SA) could also induce a transient increase latex yield. We recently reported that there are three aquaporin genes, HbPIP2;1, HbT1P1;1 and HbPIP1;1, that are regulated differentially after ethylene bark treatment. HbPIP2:1 was up-regulated in both the laticifers and the inner liber tissues, whereas HbT1P1:1 was up-regulated in the latex cells, but very markedly down-regulated in the inner liber tissues. Conversely, HbPIP1:1 was down-regulated in both tissues. In the present study, HbPIP2;1 and HbT1P1:1 showed a similar expression in response to auxin, ABA and SA, as seen in ethylene stimulation, while HbPIP1;1 was slightly regulated by auxin, but neither by ABA nor SA. The analysis of the HbPIP1:1 promoter region indicated the presence of only ethylene and auxin responsive elements. In addition, the poor efficiency of this HbPIP1;1 in increasing plasmalemma water conductance was confirmed in Xenopus oocytes. Thus, an increase in latex yield in response to all of these hormones was proposed to be the major function of aquaporins, HbPIP2:1 and HbTIP1;1. This study emphasized that the circulation of water between the laticifers and their surrounding tissues that result in latex dilution, as well as the probable maintenance of the liber tissues turgor pressure, favor the prolongation of latex flow. (C) 2010 Elsevier GmbH. All rights reserved.

    DOI: 10.1016/j.jplph.2010.06.009

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  • Early response in water relations influenced by NaCl reflects tolerance or sensitivity of barley plants to salinity stress via aquaporins

    Maki Katsuhara, Ji Ye Rhee, Genki Sugimoto, Gap Chae Chung

    SOIL SCIENCE AND PLANT NUTRITION   57 ( 1 )   50 - 60   2011年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Barley varieties, K305 and I743, which are sodium chloride (NaCl) tolerant and sensitive respectively, were hydroponically grown to determine the short-term effects of NaCl on the cell water relations in roots using a cell pressure probe, and on the transcript levels of 10 barley PIP aquaporin genes (HvPIPs) in roots. Stomatal conductance, as an indicator of sensitivity to NaCl, was decreased to less than half values of control upon exposure to 100 mmol L-1 NaCl for 24 h in I743 whereas tolerant variety, K305, was able to maintain original conductance. Osmotic half-times of water exchange in cortical cells allowed for a clear distinction between the two varieties up to 200 mmol L-1 NaCl. With treatment duration of up to 12 h with 100 mmol L-1 NaCl, the elastic modulus was reduced in I743 but increased in K305. Hydrostatic half-times of water exchange in K305 increased rapidly, whereas this value remained unchanged in I743. Application of abscisic acid (ABA) after 1 h NaCl treatment restored the hydraulic conductivity of cells (Lp) in K305 but not in I743 whereas the opposite results were obtained when mercury chloride (HgCl2) was applied, verifying the contrasting gating response of aquaporins in two varieties. Reduced expression of HvPIPs was consistent with the reduction of hydraulic conductivity of both varieties after 24 h NaCl, but without any significant differences between them, indicating the importance of the activities of existing aquaporins rather than de novo synthesis to cope with short-term effects of salt stress.

    DOI: 10.1080/00380768.2010.541870

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  • Water and H2O2 Permeability of Aquaporins Isolated from Cucumber and Figleaf Gourd. (共著)

    Rhee, J, Katsuhara, M, Kang, H. S, Chung, G. C

    Horticulture Environmental and Biotechnology   51 ( 3 )   167 - 172   2010年6月

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    記述言語:英語  

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  • Differential sodium and potassium transport selectivities of the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells. (共著)

    Yao, X, Horie, T, Xue, S, Leung, H-Y, Katsuhara, M, Brodsky, D.E, Wu, Y, Schroeder, J.I

    Plant Physiology   152 ( 1 )   341 - 355   2010年1月

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  • Insight into the salt tolerance mechanism in barley (Hordeum vulgare) from the comparisons of cultivars that differ in salt sensitivity. (共著)

    Ligaba, A, Katsuhara, M

    Journal of Plant Research   123 ( 1 )   341 - 355   2010年

  • Involvement of HbPIP2;1 and HbTIP1;1 Aquaporins in Ethylene Stimulation of Latex Yield through Regulation of Water Exchanges between Inner Liber and Latex Cells in Hevea brasiliensis

    Kessarin Tungngoen, Panida Kongsawadworakul, Unchera Viboonjun, Maki Katsuhara, Nicole Brunel, Soulaiman Sakr, Jarunya Narangajavana, Herve Chrestin

    PLANT PHYSIOLOGY   151 ( 2 )   843 - 856   2009年10月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Natural rubber is synthesized in specialized articulated cells (laticifers) located in the inner liber of Hevea brasiliensis. Upon bark tapping, the laticifer cytoplasm (latex) is expelled due to liber tissue turgor pressure. In mature virgin (untapped) trees, short-term kinetic studies confirmed that ethylene, the rubber yield stimulant used worldwide, increased latex yield, with a concomitant decrease in latex total solid content, probably through water influx in the laticifers. As the mature laticifers are devoid of plasmodesmata, the rapid water exchanges with surrounding liber cells probably occur via the aquaporin pathway. Two full-length aquaporin cDNAs (HbPIP2;1 and HbTIP1;1, for plasma membrane intrinsic protein and tonoplast intrinsic protein, respectively) were cloned and characterized. The higher efficiency of HbPIP2;1 than HbTIP1;1 in increasing plasmalemma water conductance was verified in Xenopus laevis oocytes. HbPIP2;1 was insensitive to HgCl(2). In situ hybridization demonstrated that HbPIP2;1 was expressed in all liber tissues in the young stem, including the laticifers. HbPIP2;1 was up-regulated in both liber tissues and laticifers, whereas HbTIP1;1 was down-regulated in liber tissues but up-regulated in laticifers in response to bark Ethrel treatment. Ethylene-induced HbPIP2;1 up-regulation was confirmed by western-blot analysis. The promoter sequences of both genes were cloned and found to harbor, among many others, ethylene-responsive and other chemical-responsive (auxin, copper, and sulfur) elements known to increase latex yield. Increase in latex yield in response to ethylene was emphasized to be linked with water circulation between the laticifers and their surrounding tissues as well as with the probable maintenance of liber tissue turgor, which together favor prolongation of latex flow.

    DOI: 10.1104/pp.109.140228

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  • New production method for greening concrete blocks.

    Katsuhara M

    Environment Solution Technology   8 ( 3 )   32 - 36   2009年

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  • Biomarkers of Green Roof Vegetation: Anthocyanin and Chlorophyll as Stress Marker Pigments for Plant Stresses of Rooftop Environments.

    Mori, I.C, Utsugi,S, Tanakamaru, S, Tani, A, Enomoto, T, Katsuhara, M

    Journal of Environmental Engineering and Management   19   21 - 27   2009年

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  • 岡山大学資源生物科学研究所における屋上緑化による建物冷却効果

    且原真木, 田中丸重美, 森泉, 谷明生, 宇都木繁子, 榎本敬, 米谷俊彦

    環境制御   31 ( 1 )   21 - 25   2009年

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  • 新しい緑化ブロック作成技術

    且原真木

    環境浄化技術   8 ( 3 )   32 - 36   2009年

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  • Cooling effect on buildings by the roof greening at Research Institute for Bioresources, Okayama University.

    Katsuhatra, M, Tanakamaru, S, Mori, I.C, Tani, A, Utsugi,S, Enomoto, T, Maitani, T

    Environment Research and Control   31 ( 1 )   21 - 25   2009年

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  • Characterization of four plasma membrane aquaporins in tulip petals: A putative homolog is regulated by phosphorylation

    Abul Kalam Azad, Maki Katsuhara, Yoshihiro Sawa, Takahiro Ishikawa, Hitoshi Shibata

    PLANT AND CELL PHYSIOLOGY   49 ( 8 )   1196 - 1208   2008年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    We suggested previously that temperature-dependent tulip (Tulipa gesneriana) petal movement that is concomitant with water transport is regulated by reversible phosphorylation of an unidentified plasma membrane intrinsic protein (PIP). In this study, four full-length cDNAs of PIPs from tulip petals were identified and cloned. Two PIPs, namely TgPIP1;1 and TgPIP1;2, are members of the PIP1 subfamily, and the remaining two PIPs, namely TgPIP2;1 and TgPIP2;2, belong to the PIP2 subfamily of aquaporins and were named according to the nomenclature of PIP genes in plants. Of these four homologs, only TgPIP2;2 displayed significant water channel activity in the heterologous expression assay using Xenopus laevis oocytes. The water channel activity of this functional isoform was abolished by mercury and was affected by inhibitors of protein kinase and protein phosphatase. Using a site-directed mutagenesis approach to substitute several serine residues with alanine, and assessing water channel activity using the methylotrophic yeast Pichia pastoris expression assay, we showed that Ser35, Ser116 and Ser274 are the putative phosphorylation sites of TgPIP2;2. Real-time reverse transcriptionPCR analysis revealed that the transcript levels of TgPIP1;1 and TgPIP1;2 in tulip petals, stems, leaves, bulbs and roots are very low when compared with those of TgPIP2;1 and TgPIP2;2. The transcript level of TgPIP2;1 is negligible in roots, and TgPIP2;2 is ubiquitously expressed in all organs with significant transcript levels. From the data reported herein, we suggest that TgPIP2;2 might be modulated by phosphorylation and dephosphorylation for regulating water channel activity, and may play a role in transcellular water transport in all tulip organs.

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  • Barley plasma membrane intrinsic proteins (PIP aquaporins) as water and CO(2) transporters

    Maki Katsuhara, Yuko T. Hanba

    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY   456 ( 4 )   687 - 691   2008年7月

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    記述言語:英語   出版者・発行元:SPRINGER  

    We identified barley aquaporins and demonstrated that one, HvPIP2;1, transports water and CO(2). Regarding water homeostasis in plants, regulations of aquaporin expression were observed in many plants under several environmental stresses. Under salt stress, a number of plasma membrane-type aquaporins were down-regulated, which can prevent continuous dehydration resulting in cell death. The leaves of transgenic rice plants that expressed the largest amount of HvPIP2;1 showed a 40% increase in internal CO(2) conductance compared with leaves of wild-type rice plants. The rate of CO(2) assimilation also increased in the transgenic plants. The goal of our plant aquaporin research is to determine the key aquaporin species responsible for water and CO(2) transport, and to improve plant water relations, stress tolerance, CO(2) uptake or assimilation, and plant productivity via molecular breeding of aquaporins.

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  • Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants

    Majid Mahdieh, Akbar Mostajeran, Tomoaki Horie, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   49 ( 5 )   801 - 813   2008年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Plasma membrane intrinsic proteins (PIPs), a type of aquaporins, mediate water transport in many plant species. In this study, we investigated the relationship between the functions of PIP-type water channels and water relations of tobacco plants (Nicotiana tabacum cv. Samsun) under drought stress. Drought stress treatments have led to reductions in the stomatal conductance, transpiration, water potential and turgor pressure in leaves, and also the sap flow rate and osmotic hydraulic conductance in roots. In contrast, leaf osmotic pressure was increased in response to drought stress. Interestingly, the accumulation of NtPIP1;1 and NtPIP2;1 transcripts was significantly decreased, but only that of the NtAQP1 transcript was increased under drought stress. Functional analysis using Xenopus laevis oocytes revealed that NtPIP2;1 shows marked water transport activity, but the activities of NtAQP1 and NtPIP1;1 are weak or almost negligible, respectively, when expressed alone. However, co-expression of NtPIP1;1 with NtPIP2;1 significantly enhanced water transport activity compared with that of NtPIP1;1- or NtPIP2;1-expressing oocytes, suggesting that these two aquaporins may function as a water channel, forming a heterotetramer. Heteromerization of NtPIP1;1 and NtPIP2;1 was also suggested by co-expression analyses of NtPIP1;1-GFP (green fluorescent protein) and NtPIP2;1 in Xenopus oocytes. Re-watering treatments recovered water relation parameters and the accumulation of the three NtPIP transcripts to levels similar to control conditions. These results suggest that NtPIP1;1 and NtPIP2;1 play an important role in water transport in roots, and that expression of NtPIP1;1 and NtPIP2;1 is down-regulated in order to reduce osmotic hydraulic conductance in the roots of tobacco plants under drought stress.

    DOI: 10.1093/pcp/pcn054

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  • Presence of aquaporin and V-ATPase on the contractile vacuole of Amoeba proteus

    Eri Nishihara, Etsuo Yokota, Akira Tazaki, Hidefurni Orii, Maki Katsuhara, Kensuke Kataoka, Hisako Igarashi, Yoshinori Moriyama, Teruo Shimmen, Seiji Sonobe

    BIOLOGY OF THE CELL   100 ( 3 )   179 - 188   2008年3月

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    記述言語:英語   出版者・発行元:PORTLAND PRESS LTD  

    Background information. The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90].
    Results. In the present study, we cloned an AQP gene from A. proteus [ApAQP (A. proteus AQP)] that encodes a 295-amino-acid protein. The protein has six putative TMs (transmembrane domains) and two NPA (Asn-Pro-Ala) motifs, which are conserved among various AQPs and are thought to be involved in the formation of water channels that span the lipid bilayer. Using Xenopus oocytes, we have demonstrated that the ApAQP protein product can function as a water channel. Immuncifluorescence microscopy with anti-ApAQP antibody revealed that ApAQP is detected on the CV membrane and on the vesicles around the CV. The presence of V-ATPase (vacuolar H+-ATPase) on the vesicle membrane around the CV was also detected.
    Conclusions. Our data on ApAQP allow us to provide the first informed explanation of the high water permeability of the CV membrane in amoeba. Moreover, the results suggest that vesicles possessing V-ATPase are involved in generating an osmotic gradient. Based on our findings, we propose a new hypothesis for the mechanism of CV function.

    DOI: 10.1042/BC20070091

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  • Female mating receptivity inhibited by injection of male-derived extracts in Callosobruchus chinensis

    Takashi Yamane, Yoshinobu Kimura, Maki Katsuhara, Takahisa Miyatake

    JOURNAL OF INSECT PHYSIOLOGY   54 ( 2 )   501 - 507   2008年2月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: < 3, 3-14, and > 14 kDa, the filtrate containing MW substances < 3 kDa reduced female receptivity 3 h and I day after injection, whereas the fraction containing MW substances > 14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C chinensis. (c) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2007.11.009

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  • Expanding roles of plant aquaporins in plasma membranes and cell organelles.

    Katsuhara, M, Hanba, T.Y, Shiratake, K, Maeshima, M

    Functional Plant Biology   35 ( 1 )   1 - 14   2008年

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  • Barley root hydraulic conductivity and aquaporins expression in relation to salt tolerance

    Maki Katsuhara, Mineo Shibasaka

    SOIL SCIENCE AND PLANT NUTRITION   53 ( 4 )   466 - 470   2007年8月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    Root hydraulic conductivity (Lp(r)) and aquaporins were investigated in barley seedlings under salt stress of 100 mmol L-1 NaCl in hydroponic culture for 24 and 48 h. No reduction in root elongation was observed in stressed seedlings, and more that 75% of root and shoot growth was maintained under salt stress of 100 mmol L-1 NaCl. A slight increase in HvPIP2;1 transcript, which was the most abundant of the three aquaporins investigated, was observed. No significant changes in LPr and HvPIP2;1 protein were detected. The possible reason for these complicated results and the function of aquaporins in LPr under salt stress are discussed.

    DOI: 10.1111/j.1747-0765.2007.00154.x

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  • An efflux transporter of silicon in rice

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

    NATURE   448 ( 7150 )   209 - U12   2007年7月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is an important nutrient for the optimal growth and sustainable production of rice(1-4). Rice accumulates up to 10% silicon in the shoot, and this high accumulation is required to protect the plant from multiple abiotic and biotic stresses(1-5). A gene, Lsi1, that encodes a silicon influx transporter has been identified in rice(6). Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1. This gene is constitutively expressed in the roots. The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells. Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter. The identification of this silicon transporter revealed a unique mechanism of nutrient transport in plants: having an influx transporter on one side and an efflux transporter on the other side of the cell to permit the effective transcellular transport of the nutrients.

    DOI: 10.1038/nature05964

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  • Molecular mechanisms of water uptake and transport in plant roots: research progress with water channel aquaporins.

    Katsuhara, M

    Plant Root   1   22 - 26   2007年

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  • The BnALMT1 protein that is an aluminum-activated malate transporter is localized in the plasma membrane.

    Ligaba, A, Katsuhara, M, Sakamoto, W, Matsumoto, H

    Plant signaling & behavior   2   1 - 2   2007年

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  • Comparison of transpiration rate of naturalized and domestic Sedum species.

    Mori, C.I, Enomoto, T, Katsuhara, M

    ITE Letters on batteries, New Technologies and Medicine   8   434 - 438   2007年

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  • An Aluminum-Activated Citrate Transporter in Barley.

    Furukawa, J, Yamaji, N, Wang, H, Mitani, N, Murata, Y, Sato, K, Katsuhara, M, Takeda, K, Ma, J.F

    Plant and Cell Physiology   48   1081 - 1091   2007年

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  • Low temperature and aquaporins, a molecular mechanism of water transport.

    Katsuhara, M, Chung, G.C, Sakurai, J, Murai, M, Izumi, Y, Tsumuki, H

    Cryobiology and Cryotechnology   53 ( 1 )   21 - 32   2007年

  • The BnALMT1 and BnALMT2 genes from rape encode aluminum-activated malate transporters that enhance the aluminum resistance of plant cells

    Ayalew Ligaba, Maki Katsuhara, Peter R. Ryan, Mineo Shibasaka, Hideaki Matsumoto

    PLANT PHYSIOLOGY   142 ( 3 )   1294 - 1303   2006年11月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    The release of organic anions from roots can protect plants from aluminum (Al) toxicity and help them overcome phosphorus ( P) deficiency. Our previous findings showed that Al treatment induced malate and citrate efflux from rape ( Brassica napus) roots, and that P deficiency did not induce the efflux. Since this response is similar to the malate efflux from wheat ( Triticum aestivum) that is controlled by the TaALMT1 gene, we investigated whether homologs of TaALMT1 are present in rape and whether they are involved in the release of organic anions. We isolated two TaALMT1 homologs from rape designated BnALMT1 and BnALMT2 (B. napus Al-activated malate transporter). The expression of these genes was induced in roots, but not shoots, by Al treatment but P deficiency had no effect. Several other cations ( lanthanum, ytterbium, and erbium) also increased BnALMT1 and BnALMT2 expression in the roots. The function of the BnALMT1 and BnALMT2 proteins was investigated by heterologous expression in cultured tobacco ( Nicotiana tabacum) cells and in Xenopus laevis oocytes. Both transfection systems showed an enhanced capacity for malate efflux but not citrate efflux, when exposed to Al. Smaller malate fluxes were also activated by ytterbium and erbium treatment. Transgenic tobacco cells grew significantly better than control cells following an 18 h treatment with Al, indicating that the expression of BnALMT1 and BnALMT2 increased the resistance of these plant cells to Al stress. This report demonstrates that homologs of the TaALMT1 gene from wheat perform similar functions in other species.

    DOI: 10.1104/pp.106.085233

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  • A silicon transporter in rice

    JF Ma, K Tamai, N Yamaji, N Mitani, S Konishi, M Katsuhara, M Ishiguro, Y Murata, M Yano

    NATURE   440 ( 7084 )   688 - 691   2006年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is beneficial to plant growth and helps plants to overcome abiotic and biotic stresses by preventing lodging ( falling over) and increasing resistance to pests and diseases, as well as other stresses(1-3). Silicon is essential for high and sustainable production of rice(4), but the molecular mechanism responsible for the uptake of silicon is unknown. Here we describe the Low silicon rice 1 (Lsi1) gene, which controls silicon accumulation in rice, a typical silicon-accumulating plant. This gene belongs to the aquaporin family(5) and is constitutively expressed in the roots. Lsi1 is localized on the plasma membrane of the distal side of both exodermis and endodermis cells, where casparian strips are located. Suppression of Lsi1 expression resulted in reduced silicon uptake. Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only. The identification of a silicon transporter provides both an insight into the silicon uptake system in plants, and a new strategy for producing crops with high resistance to multiple stresses by genetic modification of the root's silicon uptake capacity.

    DOI: 10.1038/nature04590

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  • Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis

    M Katsuhara, T Otsuka, B Ezaki

    PLANT SCIENCE   169 ( 2 )   369 - 373   2005年8月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Reactive oxygen species (ROS)-related membrane lipid peroxidation in the root of Arabidopsis thaliana was fluorescently visualized and investigated under salt stress. In the control roots without salt stress, more fluorescence was observed in the elongating region than in the meristematic region. Salt stress of 100 mM NaCl enhanced the fluorescence in both, indicating that salt stress-induced ROS, and consequently membrane lipid peroxidation. In transgenic tobacco glutathione S-transferase over-expressing Arabidopsis (the parB plants), less fluorescence was observed than in the non-transgenic control plants. In the salt-stressed parB plant roots, the fluorescent brightness was reduced to 46% of that of the non-transgenic plant in the meristematic region. However, the inhibition of root growth was not improved in parB plants under salt stress at pH 5.7. That is, 100 mM of salt stress reduced the root growth to 40% or less both in the parent control plants and the parB plants. The root tissue osmotic pressure was almost the same between the two tested lines, which may be one of the reasons why no difference was observed in root growth between the two lines. These results suggested that salt stress-induced oxidative stress, and the introduction/overexpression of a glutathione S-transferase gene may have reduced the amount of ROS but the removal of ROS was not sufficient to effect salt tolerance because salt stress also caused an osmotic imbalance reducing the root growth. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.plantsci.2005.03.030

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  • Hydraulic Conductivity and Aquaporins of Cortical Cells in Gravitropically Bending Roots of Pisum sativum L.

    N.Miyamoto, M.Katsuhara, T.Ookawa, K.Kasamo, T.Hirasawa

    Plant and Production Science   2005年

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  • A novel cyanobacterial SmtB/ArsR family repressor regulates the expression of a CPx-ATPase and a metallothionein in response to both Cu(I)/Ag(I) and Zn(II)/Cd(II)

    T Liu, S Nakashima, K Hirose, M Shibasaka, M Katsuhara, B Ezaki, DP Giedroc, K Kasamo

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 17 )   17810 - 17818   2004年4月

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    記述言語:英語   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    A novel SmtB/ArsR family metalloregulator, denoted BxmR, has been identified and characterized from the cyanobacterium Oscillatoria brevis. Genetic and biochemical evidence reveals that BxmR represses the expression of both bxa1, encoding a CPx-ATPase metal transporter, as well as a divergently transcribed operon encoding bxmR and bmtA, a heavy metal sequestering metallothionein. Derepression of the expression of all three genes is mediated by both monovalent (Ag(I) and Cu(I)) and divalent (Zn(II) and Cd(II)) heavy metal ions, a novel property among SmtB/ArsR metal sensors. Electrophoretic gel mobility shift experiments reveal that apoBxmR forms multiple resolvable complexes with oligonucleotides containing a single 12-2-12 inverted repeat derived from one of the two operator/promoter regions with similar apparent affinities. Preincubation with either monovalent or divalent metal ions induces disassembly of both the BxmR-bxa1 and BxmR-bxmR/bmtA operator/promoter complexes. Interestingly, the temporal regulation of expression of bxa1 and bmtA mRNAs is different in O. brevis with bxa1 induced first upon heavy metal treatment, followed by bmtA/bxmR. A dynamic interplay among Bxa1, BmtA, and BxmR is proposed that maintains metal homeostasis in O. brevis by balancing the relative rates of metal storage and efflux of multiple heavy metal ions.

    DOI: 10.1074/jbc.M310560200

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  • A wheat gene encoding an aluminum-activated malate transporter

    T Sasaki, Y Yamamoto, B Ezaki, M Katsuhara, SJ Ahn, PR Ryan, E Delhaize, H Matsumoto

    PLANT JOURNAL   37 ( 5 )   645 - 653   2004年3月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING LTD  

    The major constraint to plant growth in acid soils is the presence of toxic aluminum (Al) cations, which inhibit root elongation. The enhanced Al tolerance exhibited by some cultivars of wheat is associated with the Al-dependent efflux of malate from root apices. Malate forms a stable complex with Al that is harmless to plants and, therefore, this efflux of malate forms the basis of a hypothesis to explain Al tolerance in wheat. Here, we report on the cloning of a wheat gene, ALMT1 (aluminum-activated malate transporter), that co-segregates with Al tolerance in F-2 and F-3 populations derived from crosses between near-isogenic wheat lines that differ in Al tolerance. The ALMT1 gene encodes a membrane protein, which is constitutively expressed in the root apices of the Al-tolerant line at greater levels than in the near-isogenic but Al-sensitive line. Heterologous expression of ALMT1 in Xenopus oocytes, rice and cultured tobacco cells conferred an Al-activated malate efflux. Additionally, ALMT1 increased the tolerance of tobacco cells to Al treatment. These findings demonstrate that ALMT1 encodes an Al-activated malate transporter that is capable of conferring Al tolerance to plant cells.

    DOI: 10.1111/j.1365-313X.2003.01991.x

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  • Overexpression of the barley aquaporin HvPIP2;1 increases internal CO2 conductance and CO2 assimilation in the leaves of transgenic rice plants.

    Y.T.Hanba, M.Shibasaka, Y.Hayashi, T.Hayakawa, K.Kasamo, I.Terashima, M. Katsuhara

    Plant Cell Physiology   2004年

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  • 植物の根に関する諸問題-水透過性の分子機構:根における水チャネル・アクアポリンの機能-

    且原真木

    農業および園芸   79:600-605   2004年

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  • 水の吸収と輸送の分子機構:水チャネル研究の新展開

    且原真木

    根の研究   13:15-20   2004年

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  • A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

    T Liu, S Nakashima, K Hirose, Y Uemura, M Shibasaka, M Katsuhara, K Kasamo

    FEBS LETTERS   542 ( 1-3 )   159 - 163   2003年5月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmaA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(03)00370-3

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  • Over-expression of a barely aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants.

    M.Katsuhara, K.Koshio, M.Shibasaka, Y.Hayashi, T.Hayakawa, K.Kasamo

    Plant Cell Physiology   2003年

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  • Expression of an aquaporin at night in relation to the growth and root water permeability in barley seedlings.

    M.Katsuhara, K.Koshio, M.Shibasaka, K.Kasamo

    Soil Science and Plant Nutrition   2003年

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  • A novel histidine-rich CPx-ATPase from the filamentous cyanobacterium Oscillatoria brevis related to multiple-heavy-metal cotolerance

    L Tong, S Nakashima, M Shibasaka, M Katsuhara, K Kasamo

    JOURNAL OF BACTERIOLOGY   184 ( 18 )   5027 - 5035   2002年9月

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    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel gene related to heavy-metal transport was cloned and identified from the filamentous cyanobacterium Oscillatoria brevis. Sequence analysis of the gene (the Bxa1 gene) showed that its product possessed high homology with heavy-metal transport CPx-ATPases. The CPC motif, which is proposed to form putative cation transduction channel, was found in the sixth transmembrane helix. However, instead of the CXXC motif that is present in the N termini of most metal transport CPx-ATPases, Bxa1 contains a unique Cys-Cys (CC) sequence element and histidine-rich motifs as a putative metal binding site. Northern blotting and real-time quantitative reverse transcription-PCR showed that expression of Bxa1 mRNA was induced in vivo by both monovalent (Cu+ and Ag+) and divalent (Zn2+ and Cd2+) heavy-metal ions at similar levels. Experiments on heavy-metal tolerance in Escherichia coli with recombinant Bxa1 demonstrated that Bxa1 conferred resistance to both monovalent and divalent heavy metals. This is the first report of a CPx-ATPase responsive to both monovalent and divalent heavy metals.

    DOI: 10.1128/JB.184.18.5027-5035.2002

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  • Functional analysis of water channels in barley roots.

    M.Katsuhara, Y.Akiyama, K.Koshio, M.Shibasaka, K.Kasamo

    Plant Cell Physiology   2002年

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  • Isolation of barley salT gene: its relation to salt tolerance and to hormonal regulation by abscisic acid and jasmonic acid.

    M.Katsuhara, M.Yamada, K.Kasamo

    Soil Science and Plant Nutrition   2001年

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  • Expression and stress-dependent induction of potassium channel transcripts in the common ice plant.

    H.Su, D.Golldack, M.Katsuhara, C.Zhao, H.J.Bohnert

    Plant Physiology   2001年

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  • Different Mechanisms of Four Aluminum (Al)-Resistant Transgenes for Al Toxicity in Arabidopsis thaliana.

    B.Ezaki, M.Katsuhara, M.Kawamura, H.Matsumoto

    Plant Physiology   2001年

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  • 研究室・研究所めぐり(22)岡山大学・資源生物科学研究所

    且原真木

    遺伝   2000年

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  • Cell death and growth recovery of barley after transient salt stress.

    M.Katsuhara, M.Shibasaka

    Journal of Plant Research   2000年

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  • Establishment of in situ hybridization using Technovit resin in Arabidopsis thaliana.

    K.Takechi, W.Sakamoto, M.Katsuhara, M.Murata, F.Motoyoshi

    Plant Molecular Biology Reporter   1999年

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  • ATP IS ESSENTIAL FOR CALCIUM-INDUCED SALT TOLERANCE IN NITELLOPSIS-OBTUSA

    M KATSUHARA, M TAZAWA

    PROTOPLASMA   138 ( 2-3 )   190 - 192   1987年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

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講演・口頭発表等

  • A barley HvCNGC2-3, is activated by cAMP and the co-presence of external Na+ and K+, and permeates Na+ and K+ non-selectively.

    Taiwan-Japan Plant Biology 2017  2017年 

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  • 内膜系におけるアクアポリンの輸送基質と機能

    第19回植物オルガネラワークショップ  2017年 

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  • ダイコン野生種ハマダイコンにおける海岸生-琵琶湖陸封生の間での耐塩性分化

    日本植物学会第81回大会  2017年 

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  • 種子におけるオオムギ液胞膜型アクアポリン(HvTIPs)の相互作用と水輸送活性

    第58回日本植物生理学会年会  2017年 

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  • Identification of the CO2 transporters from among the plant aquaporins.

    Taiwan-Japan Plant Biology 2017  2017年 

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  • ハマダイコンにおけるセシウム蓄積特性の解析

    日本植物学会第81回大会  2017年 

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  • オオムギ原形質膜型アクアポリンPIP2 ファミリーの機能と根における発現プロファイル

    日本植物学会第80回大会  2016年 

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  • シロイヌナズナ概日時計変異体elf3 の根の水透過性

    日本植物学会第80回大会  2016年 

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  • 海浜植物ハマエンドウのエコタイプ間に見られる環境応答能力の違い

    日本植物分類学会第15回大会  2016年 

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  • 地球環境問題と形質転換作物:避けて通れない課題

    倉敷地方新農業経営者クラブ連絡協議会セミナー  2016年 

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  • Barley and rice aquaporins transporting hydrogenperoxide.

    第57回日本植物生理学会年会  2016年 

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  • A Salicornia europaea gene (SeNN43) encodes a novel short peptide that can improve salt tolerance in plants.

    第57回日本植物生理学会年会  2016年 

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  • 淡水域に隔離されたハマエンドウの耐塩性低下に関する比較研究

    日本植物学会第78回大会  2014年 

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  • 沈水植物ヤナギモにおける水輸送タンパク質PIPの働き

    日本植物生理学会2014年度年会  2014年 

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  • Plasma membrane intrinsic protein1 (PIP1) is a modulator of PIP2 water channel.

    日本植物生理学会2014年度年会  2014年 

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  • CO2輸送体スクリーニング法の開発

    日本植物生理学会2014年度年会  2014年 

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  • 琵琶湖湖岸と海浜に生育するハマエンドウにおける環境ストレスへの適応的な応答

    日本植物生理学会2014年度年会  2014年 

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  • イネOsHAK2およびOsHAK5輸送体の機能解析

    日本植物生理学会2014年度年会  2014年 

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  • CO2 permeability of PIP2 aquaporins.

    日本植物生理学会2014年度年会  2014年 

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  • The effect of aquaporin McMIPB and RsPIP2 on leaf photosynthesis of tobacco and eucalyptus plants.

    日本植物生理学会2014年度年会  2014年 

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  • Preliminary dissection of CO2 permeability of a cyanobacterium.

    日本植物生理学会2014年度年会  2014年 

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  • 過酸化水素輸送性アクアポリンの同定と機能/発現解析

    日本植物学会第78回大会  2014年 

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  • 琵琶湖に隔離されたハマエンドウにおける耐塩性低下の検証

    植物分類学会第13回大会  2014年 

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  • Plant aquaporin: A molecular regulator in plant water relations.

    The 1st Okayama University and King Faisal University's Workshop on Environmental and Life Science.  2014年 

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  • 植物細胞の水チャネルの調節

    神谷宣郎先生生誕百周年記念シンポジウム  2013年 

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  • 塩生植物アッケシソウの新規耐塩性遺伝子の機能スクリーニング

    第54日本植物生理学会年会  2013年 

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  • イネの水利用、成長、アクアポリン発現におよぼす低湿度と低地温の影響

    第54日本植物生理学会年会  2013年 

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  • 種子特異的に発現するオオムギTIP3;1による水輸送活性の調節

    第54日本植物生理学会年会  2013年 

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  • 琵琶湖に陸封されたハマエンドウと海浜集団の間に生じた光合成特性の分化

    第54日本植物生理学会年会  2013年 

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  • 琵琶湖に隔離されたハマエンドウと海浜集団の間におけるABAを介した光合成特性の分化

    日本植物学会第77回大会  2013年 

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  • それではいかに放射性物質に対応するか:植物科学からの取り組み

    吉備の国クラスター・エコ環境グループ講演会  2013年 

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  • 水輸送とCO2輸送の分子基盤:アクアポリン

    日本植物学会第77回大会シンポジウム  2013年 

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  • 原形質膜型アクアポリンPIP1とPIP2の共発現による活性抑制

    第54日本植物生理学会年会  2013年 

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  • アクアポリンのCO2透過性

    第54日本植物生理学会年会  2013年 

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  • イネアクアポリンOsPIPの発現と根水透過性:浸透圧ストレスの影響および形質転換での解析

    第54日本植物生理学会年会  2013年 

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  • Root hydraulic conductivity and PIP aquaporins of barely and rice in response to salinity/osmotic stress.

    植物膜生物学国際学会2013  2013年 

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  • 重金属イオンによる植物アクアポリン阻害作用についての検討

    第54日本植物生理学会年会  2013年 

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  • Effect of low root-temperature on water use, growth and aquaporin expression in rice plants under various air-humidity conditions.

    植物膜生物学国際学会2013  2013年 

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  • The functional analysis of Cyclic-Nucleotide Gated Cation Channels (CNGCs) in barley.

    植物膜生物学国際学会2013  2013年 

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  • The photosynthetic response of tobacco and eucalyptus plants overexpressing aquaporins.

    植物膜生物学国際学会2013  2013年 

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  • Gating of plasma membrane intrinsic protein1 via interaction with plasma membrane intrinsic protein2.

    植物膜生物学国際学会2013  2013年 

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  • The Third Type of PIP Aquaporins.

    日本植物生理学会2012年度年会  2012年 

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  • オオムギとイネの根水透過性制御と浸透圧ストレス応答

    第36回根研究集会  2012年 

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  • Regulation of root hydraulic conductivity in gramineous plants under osmotic stress.

    日本植物生理学会2012年度年会  2012年 

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  • オオムギにおけるNa輸送性CNGCチャネルの機能解析

    日本土壌肥料学会2012年度鳥取大会  2012年 

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  • Osmotic-induced reduction of root hydraulic conductivity is essential as an early response in slat/osomotic tolerance

    ゴードン科学会議 植物の塩と水ストレス  2012年 

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  • イネとオオムギの亜ヒ酸輸送性NIP型アクアポリン

    日本植物学会第76回大会  2012年 

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  • 水生植物ヤナギモにおける水輸送タンパク質PIPの解析

    日本植物学会第76回大会  2012年 

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  • Dynamic regulation of root hydraulic conductivity and aquaporins under salt stress.

    International symposium, Strategies of Plants against Global Environmental Change.  2011年 

     詳細を見る

  • Detection of transport activity in barley and rice aquaporins with newly developed yeast system.

    日本植物生理学会2011年度年会  2011年 

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  • Hydrogen peroxide permeability of aquaporin in rice, barley and Arabidopsis

    第3回植物アクアポリン研究会  2011年 

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  • 形質膜アクアポリンPIP1の水チャンネルはPIP2によって開けられる

    日本植物生理学会2011年度年会  2011年 

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  • アクアポリンのCO2透過性

    第3回植物アクアポリン研究会  2011年 

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  • 浸透圧ストレス環境下におけるオオムギの水利用機能研究

    第3回植物アクアポリン研究会  2011年 

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  • 亜ヒ酸輸送活性を持つイネおよびオオムギのアクアポリン

    第3回植物アクアポリン研究会  2011年 

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  • オオムギの根水透過性とアクアポリンの浸透圧ストレス応答

    第3回植物アクアポリン研究会  2011年 

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  • 原形質膜局在型アクアポリンの共発現による活性化の分子機構

    第3回植物アクアポリン研究会  2011年 

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  • 浸透圧ストレス環境下におけるイネ科作物の水利用機能研究

    第35回根研究集会  2011年 

     詳細を見る

  • Dynamic regulation of root hydraulic conductivity and aquaporins under salt stress.

    The JSRR's 20th Anniversary Symposium  2011年 

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  • 緑化ブロックと屋上緑化の新展開

    第二回かんきょうひろば全体交流会  2011年 

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  • オオムギPIP1型と2型アクアポリンのへテロマー形成の分子機構

    日本植物生理学会2010年度年会  2010年 

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  • 植物における水輸送の分子基盤・アクアポリン:動物との共通点と相違点

    3部局合同セミナー  2010年 

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  • 原形質膜局在型アクアポリンPIP1とPIP2の共発現による活性化メカニズムの解析

    日本植物生理学会2010年度年会  2010年 

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  • 緑化ブロックの開発

    第4回産学官民コミュニティー全国大会 in おかやま  2010年 

     詳細を見る

  • Regulation of Root Water Permeability in Barley under Salt Stress.

    ゴードン科学会議(植物の塩およびお水ストレス)  2010年 

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  • Cooling effect on buildings by the roof greening at Institute of Plant Science and Resources, Okayama University.

    第5回ジョモケニアッタ農工大学科学・技術;産業会議  2010年 

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  • Studies on water channel activity of a PIP1 aquaporin and its interaction with PIP2 aquaporins.

    第15回国際植物膜生物学ワークショップ  2010年 

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  • Water transport in plants under stresses: from molecules to whole plant.

    第5回ジョモケニアッタ農工大学科学・技術;産業会議  2010年 

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  • 高浸透圧ストレス下におけるオオムギ根の水輸送活性制御機構の解明

    日本植物生理学会2010年度年会  2010年 

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  • Aquaporin - molecular mechanism of water transport in plants: Common and different characteristics between animals and plants.

    2010年 

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  • 水と植物の2つの視点から 見えること 考えること

    第四回岡山大学いちょう並木研究サロン  2009年 

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  • 原形質膜局在型アクアポリンPIP1とPIP2の共発現による活性化メカニズムの解析

    日本植物生理学会2009年度年会  2009年 

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  • オオムギアクアポリンの機能と塩ストレス応答

    日本植物生理学会2009年度年会  2009年 

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  • オオムギ根の水輸送活性は塩ストレス下において翻訳後修飾によって抑制されている

    日本植物生理学会2009年度年会  2009年 

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  • Varietal differences in the rates of root growth and leaf photosynthesis, hydraulic conductivity and expressions of aquaporins in barley seedlings under salt stress conditions.

    PlantBiology2009  2009年 

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  • 花と果実におけるアクアポリンの発現・調節と機能

    日本植物生理学会2009年度年会  2009年 

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  • アクアポリンが光合成機能に果たす役割の定量的評価

    日本生態学会近畿地区会2009年第2回例会  2009年 

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  • 高等植物の光合成と水チャネル

    第55回日本生態学会福岡大会  2008年 

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  • マメゾウムシ二種におけるオスによるメスの交尾抑制の変異と種間交尾がメスの再交尾に与える影響

    第55回日本生態学会福岡大会  2008年 

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  • Xenopus oocyteとPichia pastorisで発現させたチューリップ花弁由来アクアポリンの水輸送活性:ホモログTgPIP2;2はリン酸化によって制御される

    日本植物生理学会2008年度年会  2008年 

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  • Water transport in rice and Graminean plants: from whole plant to aquaporins.

    Beijing Botanical Society Workshop  2008年 

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  • 草本植物の茎葉では機械的ストレスを水分移動によって緩和している

    日本植物生理学会2008年度年会  2008年 

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  • オオムギ原形質膜型アクアポリンの発現制御と水輸送特性

    日本植物生理学会2008年度年会  2008年 

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  • マメゾウムシ2種におけるオスの交尾抑制物質の生化学的分析

    第52回日本応用動物昆虫学会大会  2008年 

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  • セイヨウナシアクアポリンPcPIP2;2のリン酸化による水輸送活性調節

    日本植物生理学会2008年度年会  2008年 

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  • タバコアクアポリンの機能・発現と根の水透過性の制御

    第28回根研究会  2008年 

     詳細を見る

  • 屋上緑化の現状と展望:資源生物科学研究所の取り組みと研究開発

    吉備の国クラスター・エコ環境グループ講演会  2008年 

     詳細を見る

  • 植物と水と私たち

    第53回大原孫三郎總一郎記念講演会  2008年 

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  • オオムギ根における水透過性測定のためのpressure chamber作成と測定法の確立

    第28回根研究会  2008年 

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  • プロジェクト概要と現在の活動について

    岡山大学資源生物科学研究所屋上緑化プロジェクト公開報告・見学会  2008年 

     詳細を見る

  • コンクリート製緑化ブロック

    平成20年度第1回TLOシーズ説明会  2008年 

     詳細を見る

  • Transcriptional Regulations and Water Transport Activity of PIP-type Aquaporins in Roots of Barley.

    Gordon Research Conference - Salt & Water Stress In Plants  2008年 

     詳細を見る

  • Phosphorylational regulation of plasma membrane aquaporin, PIP2, and its physiological roles in fruit and flower.

    FESPB2008  2008年 

     詳細を見る

  • 塩ストレス条件下におけるオオムギ幼植物の根の水伝道度と光合成速度の品種間差

    日本作物学会第226回講演会  2008年 

     詳細を見る

  • PIP aquaporins and water relations in barley roots under salt stress.

    Gordon Research Conference ? Salt & Water Stress In Plants  2008年 

     詳細を見る

  • セイヨウナシのアクアポリンPIP2のリン酸化による活性調節と果実日肥大におけるリン酸化状態の変化

    園芸学会平成20年度秋季大会  2008年 

     詳細を見る

  • オオムギ原形質膜型アクアポリンの発現と機能および塩ストレスと重金属の影響の解析

    日本植物学会第72回大会  2008年 

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  • 塩ストレス条件下におけるオオムギ根の水透過性

    第29回根研究会  2008年 

     詳細を見る

  • 耐塩性の異なるオオムギ2品種の根における原形質膜型アクアポリンの発現

    第29回根研究会  2008年 

     詳細を見る

  • シロイヌナズナの浸透圧ストレス誘導性糖トランスポーターERD6の機能解析

    第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会  2008年 

     詳細を見る

  • Aquaporins mediate the transports of essential molecules in plants growing in various environments.

    5th International Conference of Aquaporin  2007年 

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  • The effect of aquaporin HvPIP2;1 and McMIPA on leaf photosynthesis for rice and tobacco plants.

    5th International Conference of Aquaporin (Nara, Japan) 2007年7月13日-16日  2007年 

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  • オオムギ原形質膜型アクアポリン遺伝子の同定と塩ストレスによる発現制御

    日本植物学会第71回大会  2007年 

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  • Multi-functional plant aquaporins mediating transports of water and essential molecules for plant life.

    Special Lectures by Professors in Sister Research (Agricultural Plant Stress Research Center, Chonnam National University, Korea)  2007年 

     詳細を見る

  • 緑化ブロックと建物緑化新技術の開発

    岡山大学知恵の見本市2007  2007年 

     詳細を見る

  • オオムギ原形質膜型アクアポリン遺伝子の同定と塩ストレスによる発現制御

    第27回根研究会  2007年 

     詳細を見る

  • アズキゾウムシにおけるオス由来のメス交尾抑制物質の生化学的分析

    第51回日本応用動物昆虫学会  2007年 

     詳細を見る

  • 植物のアクアポリンの多彩な役割とその制御

    第9回植物生体膜シンポジウム「植物の生理機能と生理学的構造の役割」(  2007年 

     詳細を見る

  • イネ科NIPのケイ酸輸送特性の解析

    日本植物生理学会2007年度年会  2007年 

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  • オオムギのアルミニウム活性型クエン酸輸送体候補遺伝子の発現ならびに機能解析

    日本植物生理学会2007年度年会  2007年 

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  • 塩ストレスの初期段階におけるイネアクアポリン発現の同調的変動

    日本植物生理学会2007年度年会(愛媛) 2007年3月28日-30日  2007年 

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  • 在来および帰化マンネングサ属の水利用効率の比較

    日本植物生理学会2007年度年会  2007年 

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  • オオムギ液胞膜型アクアポリン遺伝子の環境ストレスによる発現調節

    日本植物生理学会2007年度年会(愛媛) 2007年3月28日-30日  2007年 

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  • Salt and osmotic stress regulates differentially plasma-membrane-type aquaporin gene expression in salt tolerant and salt sensitive barley.

    Gordon Research Conference – Salt & Water Stress In Plants  2006年 

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  • Cloning and Functional Analysis of Plasma Membrane Aquaporin Subfamilies of Tulip Petal.

    PlantBiology2006  2006年 

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  • ヒ素と重金属に応答する植物モニター系の構築

    岡山大学・学内COE「資源生物を用いた地球環境のモニター系の構築と環境保全への応用」国際ワークショップ  2006年 

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  • アクアポリンの水ストレスへの応答と多彩な機能

    東北農業研究セミナー「生命を支える分子システム-植物の水吸収をになう「アクアポリン」研究の最前線」  2006年 

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  • A molecular investigation of an aluminium-induced malate secretion from rape (Brassica napus L.).

    PlantBiology2006  2006年 

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  • アクアポリン分子の基質輸送に伴う分子骨格構造の変動

    日本植物生理学会2006年度年会  2006年 

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  • Cloning and functional analysis of an aluminium-induced malate transporter gene from rape (Brassica napus).

    日本植物生理学会2006年度年会  2006年 

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  • 水輸送の分子機構・アクアポリンと低温

    第52回低温生物工学会セミナー  2006年 

     詳細を見る

  • 水環境とオオムギの遺伝子発現制御

    日本植物生理学会2006年度年会  2006年 

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  • Functional and molecular analysis of the barley Ca2+/H+ antiporter.

    Plant Biology2005  2005年 

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  • 岡山県南部に適化した薄層省管理緑化の新システムの開発

    大学発新事業創出促進事業「技術シーズ発信会」  2005年 

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  • オオムギのCDPK遺伝子および液胞膜型CAX遺伝子の同定と機能解析

    第69回日本植物学会  2005年 

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  • Osmotic stress regulates differentially aquaporin genes expression in salt tolerant and salt sensitive barley (Hordeum vulgare).

    4th Plant Genomic European Meetings  2005年 

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  • 水チャネル・MIP遺伝子ファミリーの多様な機能

    第1回ムギ類研究会  2005年 

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  • オオムギの液胞膜型Cation/H+ exchanger (CAX)

    第8回植物生体膜シンポジウム  2005年 

     詳細を見る

  • アクアポリンの多様性と多面性:耐塩性や光合成との関係 (シンポジウム アクアポリンの多様性:構造、機能、局在)

    日本植物生理学会2005年度年会シンポジウム  2005年 

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  • イネゲノム中の全てのPIPファミリー遺伝子

    日本植物生理学会2005年度年会  2005年 

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  • Transcriptional regulation of plasma membrane intrinsic protein (PIP) aquaporins in barley (Hordeum vulgare ,Haruna-nijo) by heavy metals.

    10th International Congress SABRAO  2005年 

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  • オオムギ根の水輸送とアクアポリンファミリー

    第21回根研究集会  2004年 

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  • 屈性を示している根の細胞の水吸収

    第1回植物アクアポリン研究討論会  2004年 

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  • オオムギ水チャネルとその塩ストレス応答についての解析

    第1回植物アクアポリン研究討論会  2004年 

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  • オオムギアクアポリンHvPIP2;1を過剰発現させた形質転換イネ葉での二酸化炭素拡散コンダクタンスと光合成速度の上昇

    第1回植物アクアポリン研究討論会  2004年 

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  • Regulation of malate efflux in wheat root apex under aluminum stress: role of protein phosphorylation and ALMT1 gene.

    The 1st International Symposium "Life and Environmental Science in Future"  2004年 

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  • 水チャンネル過剰発現イネにおける根の形質と塩ストレス応答性の変化

    日本植物生理学会2004年度年会  2004年 

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  • 植物細胞におけるイオンと水:その生理と輸送機構の動物細胞との共通点と相違点

    第47回日本腎臓学会学術総会  2004年 

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  • オオムギ根のアクアポリンと水輸送、および塩ストレスとの関係

    第68回日本植物学会  2004年 

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  • Aquaporins under salt stress

    Gordon Research Conference "Salt and Water Stress in Plants"  2004年 

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  • オオムギにおけるストレス応答性遺伝子MAPKおよびCAXの単離と機能解析

    第21回根研究集会  2004年 

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  • グルタチオン包合体の液胞膜透過機構に関するABCトランスポーター遺伝子

    第68回日本植物学会  2004年 

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  • 植物のアクアポリンの働き

    第19回「大学と科学」公開シンポジウム みずみずしい体のしくみ:水の通り道“アクアポリン”の働きと病気  2004年 

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  • Increase in leaf CO2 conductance and decrease in salt tolerance in transgenic rice plant over-expressing barley aquaporin.

    Plant Biology 2003  2003年 

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  • タバコ由来のグルタチオン-S-トランスフェラーゼ遺伝子(parB)の導入によって活性酸素除去系を強化した形質転換シロイヌナズの塩ストレスに対する応答

    日本植物学会第67回大会  2003年 

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  • オオムギ幼根の水透過性に関連する水チャネルの夜間における発現

    第19回根研究集会  2003年 

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  • オオムギ水チャンネルは二酸化炭素を透過する-形質転換イネ葉での二酸化炭素拡散コンダクタンスの上昇-

    関西光合成研究会  2003年 

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  • オオムギの水輸送と塩ストレス応答

    第19回根研究集会  2003年 

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  • 重金属イオン輸送体、CPx-ATPase遺伝子(Bxa1)の単離と特性解析

    日本植物生理学会2003年度年会  2003年 

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  • コムギにおけるアルミニウム活性化型リンゴ酸トランスポーター遺伝子のアルミニウム耐性への関与

    日本植物生理学会2003年度年会  2003年 

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  • コムギのAl耐性機構-リンゴ酸分泌とそのトランスポーター遺伝子-

    第18回根研究集会  2003年 

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  • オオムギ水チャンネル遺伝子を過剰発現させた形質転換体イネ緑葉での二酸化炭素透過性(拡散コンダクタンス)の上昇

    日本植物生理学会2003年度年会  2003年 

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  • コムギのアルミニウム活性化型リンゴ酸トランスポーター遺伝子の機能解析:対立遺伝子間の機能相違

    日本土壌肥料学会2003年度神奈川大会  2003年 

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  • かび臭物質生産ラン藻Oscillatoria brevisの重金属耐性に関する分子生物学的研究

    日本水処理生物学会第39回大会  2002年 

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  • アルミニウム耐性小麦におけるリンゴ酸放出機構の分子生物学的解析

    第6回植物生体膜シンポジウム  2002年 

     詳細を見る

  • 塩ストレス抵抗性オオムギに関する研究1. 塩ストレスで特異的に発現する遺伝子の解析

    日本農芸化学会2002年度大会  2002年 

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  • 植物水チャンネルの遺伝子と機能

    第6回植物生体膜シンポジウム  2002年 

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  • アルミニウム耐性小麦に特異的な遺伝子の機能解析

    日本土壌肥料学会2002年度名古屋大会  2002年 

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  • オオムギの水チャネルHvPIP2;1の塩ストレスおよび乾燥ストレス下における機能的発現と日周変化

    日本植物生理学会2002年度年会  2002年 

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  • かび臭物質生産ラン藻Oscillatoria brevisの多種金属耐性に関わる重金属イオン輸送体に関する研究

    日本水処理生物学会第39回大会  2002年 

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  • ラン藻Oscillatoria brevisのメタロチオネイン遺伝子の同定

    日本植物学会第65回大会  2001年 

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  • ラン藻Oscillatoria brevisからの重金属輸送遺伝子の同定と解析

    日本植物生理学会2001年度年会  2001年 

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  • オオムギ根から単離した水チャンネル遺伝子の機能発現

    日本植物生理学会2001年度年会  2001年 

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  • ラン藻Oscillatoria brevisのP-型重金属イオン輸送ATPaseの研究

    日本植物学会第65回大会  2001年 

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  • オオムギの水チャンネル遺伝子bpw1の局在性と発現の日周変化

    日本植物生理学会2001年度年会  2001年 

     詳細を見る

  • Water channels in barley roots.

    The 6th International Society of Root Research Symposium  2001年 

     詳細を見る

  • 塩ストレス誘導性細胞死と生体膜

    植物生体膜シンポジウム  2000年 

     詳細を見る

  • 植物の水チャンネルの構造と機能発現

    第17回資源生物科学シンポジウム  2000年 

     詳細を見る

  • Apoptosis-like cell death under salt stress.

    Gordon Research Conference "Cellular Basisi of Adaptation to Salt and Water Stress in Plants"  2000年 

     詳細を見る

  • Cell death and stress response in plants

    International symposium on "Stress responses in biological systems"  1999年 

     詳細を見る

  • 塩ストレス誘導性細胞死の初期過程における細胞膜インテグリティーの研究

    日本植物生理学会1999年度年会  1999年 

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  • オオムギからの水チャンネル遺伝子の単離と塩ストレス下における発現の解析

    日本植物生理学会1999年度年会 1999年3月  1999年 

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  • 耐塩性の異なるオオムギ3品種におけるsalT様遺伝子の発現制御

    日本植物学会第63回大会  1999年 

     詳細を見る

▼全件表示

Works(作品等)

  • 都市緑化おかやまフェア2009

    2009年

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受賞

  • 第5回村川技術奨励賞

    2010年  

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    受賞国:日本国

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  • 日本作物学会論文賞

    2007年  

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    受賞国:日本国

    researchmap

  • 日本植物生理学会論文賞

    2006年  

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    受賞国:日本国

    researchmap

 

担当授業科目

  • 植物分子生理学演習 (2021年度) 前期  - その他

  • 植物分子生理学演習 (2021年度) 後期  - その他

  • 植物分子生理学演習 (2021年度) 後期  - その他

  • 植物分子生理学演習 (2021年度) 前期  - その他

  • 植物生理機能学 (2021年度) 前期  - その他

  • 環境応答生理学 (2021年度) 前期  - 水1~4

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 植物分子生理学演習 (2020年度) 前期  - その他

  • 植物分子生理学演習 (2020年度) 後期  - その他

  • 植物分子生理学演習 (2020年度) 後期  - その他

  • 植物分子生理学演習 (2020年度) 前期  - その他

  • 植物生理学1 (2020年度) 第3学期  - 金1,金2

  • 植物生理機能学 (2020年度) 前期  - その他

  • 環境応答生理学 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

▼全件表示