Updated on 2025/07/18

写真a

 
KATSUHARA Maki
 
Organization
Scheduled update Professor
Position
Professor
Homepage
http://www.rib.okayama-u.ac.jp/~kmaki/katu-j.htm
External link

Degree

  • Doctor of Science ( The University of Tokyo )

Research Interests

  • membrane transport

  • plant physiology

  • salt tolerance

  • stress physiology

  • 植物生理学

  • aquaporin

  • ストレス生理学

  • 膜輸送

  • アクアポリン

  • 耐塩性

Research Areas

  • Life Science / Plant nutrition and soil science

  • Life Science / Plant molecular biology and physiology

Education

  • The University of Tokyo   大学院理学系研究科植物学専攻博士課程  

    1987.4 - 1990.3

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    Country： Japan

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  • The University of Tokyo   大学院理学系研究科植物学専攻修士課程  

    1985.4 - 1987.3

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    Country： Japan

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  • The University of Tokyo   理学部   生物学教室

    1981.4 - 1985.3

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    Country： Japan

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Research History

  • Okayama University   Institute of Plant Science and Resources   Professor

    2017.4

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    Country：Japan

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  • Okayama University   Institute of Plant Science and Resources   Associate Professor

    2003.10 - 2017.3

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    Country：Japan

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  • Okayama University   Research Institute for Bioresources   Assistant Professor

    1992.4 - 2003.9

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    Country：Japan

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  • Insect Agricultural Technology Research Institute   Research Fellow

    1990.10 - 1992.3

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    Country：Japan

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  • The University of Tokyo   Faculty of Science   Post-doctoral researcher

    1990.4 - 1990.9

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    Country：Japan

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Professional Memberships

Committee Memberships

  • 鳥取大学乾燥地研究センター   運営員会委員会委員(外部委員)  

    2024.6   

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    Committee type：Other

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  • The Japanese Society of Plant Physiologists   Representatives  

    2022.1 - 2023.12   

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    Committee type：Academic society

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  • 日本植物学会   編集担当理事  

    2021.3 - 2025.3   

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    Committee type：Academic society

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  • 根研究学会   評議員  

    2020.4   

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    Committee type：Academic society

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  • 鳥取大学乾燥地研究センター   共同研究委員会委員(外部委員)  

    2020.4 - 2024.3   

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  • 日本植物学会   編集実行委員  

    2012 - 2016   

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    Committee type：Academic society

    日本植物学会

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  • 日本植物生理学会   代議員  

    2012 - 2014   

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    Committee type：Academic society

    日本植物生理学会

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  • 根研究会   英文誌編集委員会委員  

    2007   

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    Committee type：Academic society

    根研究会

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Papers

  • The hidden cation-selective pore in ion-conducting aquaporin OsPIP2;4 from rice Reviewed

    Shuntaro Ono, Sen Thi Huong Tran, Yasunori Saitoh, Shigeko Utsugi, Tomoaki Horie, Maki Katsuhara

    Plant Physiology and Biochemistry   227   110168 - 110168   2025.10

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.plaphy.2025.110168

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  • Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of CO2 Reviewed

    Shaila Shermin Tania, Shigeko Utsugi, Yoshiyuki Tsuchiya, Shizuka Sasano, Maki Katsuhara, Izumi C. Mori

    Plant, Cell & Environment   2025.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    ABSTRACT

    The transport of CO2 across biomembranes in plant cells is essential for efficient photosynthesis. Some aquaporins capable of CO2 transport, referred to as ‘COOporins’, are postulated to play a crucial role in leaf CO2 diffusion. However, the structural basis of CO2 permeation through aquaporins remains largely unknown. Here, we show that amino acids in loop C are critical for the CO2 permeability of Arabidopsis thaliana PIP2 aquaporins. We found that swapping tyrosine and serine in loop C to histidine and phenylalanine, which differ between AtPIP2;1 and AtPIP2;3, altered CO2 permeability when examined in the Xenopus laevis oocyte heterologous expression system. AlphaFold2 modelling indicated that these substitution induced a conformational shift in the sidechain of arginine in the aromatic/arginine (ar/R) selectivity filter and in lysine at the extracellular mouth of the monomeric pore in PIP2 aquaporins. Our findings demonstrate that distal amino acid substitutions can trigger conformational changes of the ar/R filter in the monomeric pore, modulating CO2 permeability. Additionally, phylogenetic analysis suggested that aquaporins capable of dual water/CO2 permeability are ancestral to those that are water‐selective and CO2‐impermeable, and CO2‐selective and water impermeable.

    DOI： 10.1111/pce.15635

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  • OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance Reviewed

    Sen Thi Huong Tran, Maki Katsuhara, Yunosuke Mito, Aya Onishi, Ayaka Higa, Shuntaro Ono, Newton Chandra Paul, Rie Horie, Yoshihiko Harada, Tomoaki Horie

    Scientific Reports   15 ( 1 )   2025.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1038/s41598-025-96259-1

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    Other Link： https://www.nature.com/articles/s41598-025-96259-1

  • Analysis of the effect of permeant solutes on the hydraulic resistance of the plasma membrane in cells of Chara corallina Reviewed

    Masashi Tazawa, Randy Wayne, Maki Katsuhara

    Protoplasma   2024.10

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    In the cells of Chara corallina, permeant monohydric alcohols including methanol, ethanol and 1-propanol increased the hydraulic resistance of the membrane (Lpm−1). We found that the relative value of the hydraulic resistance (rLpm−1) was linearly dependent on the concentration (Cs) of the alcohol. The relationship is expressed in the equation: rLpm−1 = ρmCs + 1, where ρm is the hydraulic resistance modifier coefficient of the membrane. Ye et al. (2004) showed that membrane-permeant glycol ethers also increased Lp−1. We used their data to estimate Lpm−1 and rLpm−1. The values of rLpm−1 fit the above relation we found for alcohols. When we plotted the ρm values of all the permeant alcohols and glycol ethers against their molecular weights (MW), we obtained a linear curve with a slope of 0.014 M−1/MW and with a correlation coefficient of 0.99. We analyzed the influence of the permeant solutes on the relative hydraulic resistance of the membrane (rLpm−1) as a function of the external (π0) and internal (πi) osmotic pressures. The analysis showed that the hydraulic resistance modifier coefficients (ρm) were linearly related to the MW of the permeant solutes with a slope of 0.012 M−1/MW and with a correlation coefficient of 0.84. The linear relationship between the effects of permeating solutes on the hydraulic resistance modifier coefficient (ρm) and the MW can be explained in terms of the effect of the effective osmotic pressure on the hydraulic conductivity of water channels. The result of the analysis suggests that the osmotic pressure and not the size of the permeant solute as proposed by (Ye et al., J Exp Bot 55:449–461, 2004) is the decisive factor in a solute’s influence on hydraulic conductivity. Thus, characean water channels (aquaporins) respond to permeant solutes with essentially the same mechanism as to impermeant solutes.

    DOI： 10.1007/s00709-024-02000-6

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    Other Link： https://link.springer.com/article/10.1007/s00709-024-02000-6/fulltext.html

  • In planta evidence that the HAK transporter OsHAK2 is involved in Na+ transport in rice. Reviewed International journal

    Shigeto Morita, Natsumi Tamba, Mineo Shibasaka, Shizuka Sasano, Taiju Kadoike, Yasuyo Urase, Masamitsu Maruyama, Aguri Fukuoka, Junta Yanai, Takehiro Masumura, Yasunari Ogihara, Shigeru Satoh, Kunisuke Tanaka, Maki Katsuhara, Hideki Nakayama

    Bioscience, biotechnology, and biochemistry   87 ( 5 )   482 - 490   2023.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    HAK family transporters primarily function as K+ transporters and play major roles in K+ uptake and translocation in plants, whereas several HAK transporters exhibit Na+ transport activity. OsHAK2, a rice HAK transporter, was shown to mediate Na+ transport in Escherichia coli in a previous study. In this study, we investigated whether OsHAK2 is involved in Na+ transport in the rice plant. Overexpression of OsHAK2 increased Na+ translocation from the roots to the shoots of transgenic rice. It also increased both root and whole-plant Na+ content, and enhanced shoot length under low Na+ and K+ conditions. Meanwhile, OsHAK2 overexpression increased salt sensitivity under a long-term salt stress condition, indicating that OsHAK2 is not involved in salt tolerance, unlike in the case of ZmHAK4 in maize. These results suggest that OsHAK2 is permeable to Na+ and contributes to shoot growth in rice plants under low Na+ and K+ conditions.

    DOI： 10.1093/bbb/zbad020

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  • Distinct Functions of the Atypical Terminal Hydrophilic Domain of the HKT Transporter in the Liverwort Marchantia polymorpha. Reviewed International coauthorship International journal

    Shahin Imran, Masumi Oyama, Rie Horie, Natsuko I Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie

    Plant & Cell Physiology   63 ( 6 )   802 - 816   2022.6

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    K+/Na+ homeostasis is important for land plants, particularly under salt stress. In this study, the structure and ion transport properties of the high-affinity K+ transporter (HKT) of the liverwort Marchantia polymorpha were investigated. Only one HKT gene, MpHKT1, was identified in the genome of M. polymorpha. Phylogenetic analysis of HKT proteins revealed that non-seed plants possess HKTs grouped into a clade independent of the other two clades including HKTs of angiosperms. A distinct long hydrophilic domain was found in the C-terminus of MpHKT1. Complementary DNA (cDNA) of truncated MpHKT1 (t-MpHKT1) encoding the MpHKT_Δ596-812 protein was used to examine the functions of the C-terminal domain. Both MpHKT1 transporters fused with enhanced green fluorescent protein at the N-terminus were localized to the plasma membrane when expressed in rice protoplasts. Two-electrode voltage clamp experiments using Xenopus laevis oocytes indicated that MpHKT1 mediated the transport of monovalent alkali cations with higher selectivity for Na+ and K+, but truncation of the C-terminal domain significantly reduced the transport activity with a decrease in the Na+ permeability. Overexpression of MpHKT1 or t-MpHKT1 in M. polymorpha conferred accumulation of higher Na+ levels and showed higher Na+ uptake rates, compared to those of wild-type plants; however, phenotypes with t-MpHKT1 were consistently weaker than those with MpHKT1. Together, these findings suggest that the hydrophilic C-terminal domain plays a unique role in the regulation of transport activity and ion selectivity of MpHKT1.

    DOI： 10.1093/pcp/pcac044

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  • Calcium control of the hydraulic resistance in cells of Chara corallina Reviewed International coauthorship International journal

    Masashi Tazawa, Maki Katsuhara, Randy Wayne

    Protoplasma   260 ( 1 )   299 - 306   2022.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00709-022-01772-z

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    Other Link： https://link.springer.com/article/10.1007/s00709-022-01772-z/fulltext.html

  • Identification and Characterization of Rice OsHKT1;3 Variants Reviewed International journal

    Shahin Imran, Yoshiyuki Tsuchiya, Sen Thi Huong Tran, Maki Katsuhara

    Plants   10 ( 10 )   2006 - 2006   2021.9

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    In rice, the high-affinity K+ transporter, OsHKT1;3, functions as a Na+-selective transporter. mRNA variants of OsHKT1;3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1;3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1;3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1;3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1;3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.

    DOI： 10.3390/plants10102006

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  • Age dependence of the hydraulic resistances of the plasma membrane and the tonoplast (vacuolar membrane) in cells of Chara corallina Reviewed International coauthorship International journal

    Masashi Tazawa, Maki Katsuhara, Randy Wayne

    Protoplasma   258 ( 4 )   793 - 801   2021.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00709-020-01596-9

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    Other Link： https://link.springer.com/article/10.1007/s00709-020-01596-9/fulltext.html

  • Mechanisms Activating Latent Functions of PIP Aquaporin Water Channels via the Interaction between PIP1 and PIP2 Proteins Reviewed International journal

    Mineo Shibasaka, Tomoaki Horie, Maki Katsuhara

    Plant & Cell Physiology   62 ( 1 )   92 - 99   2021.3

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    <title>Abstract</title>
    Plant plasma membrane-type plasma membrane intrinsic protein (PIP) aquaporins are classified into two groups, PIP1s and PIP2s. In this study, we focused on HvPIP1;2, a PIP1 in barley (Hordeum vulgare), to dissect the molecular mechanisms that evoke HvPIP1-mediated water transport. No HvPIP1;2 protein was localized to the plasma membrane when expressed alone in Xenopus laevis oocytes. By contrast, a chimeric HvPIP1;2 protein (HvPIP1;2_24NC), in which the N- and C-terminal regions were replaced with the corresponding regions from HvPIP2;4, was found to localize to the plasma membrane of oocytes. However, HvPIP1;2_24NC showed no water transport activity in swelling assays. These results suggested that the terminal regions of PIP2 proteins direct PIP proteins to the plasma membrane, but the relocalization of PIP1 proteins was not sufficient to PIP1s functionality as a water channel in a membrane. A single amino acid replacement of threonine by methionine in HvPIP2;4 (HvPIP2;4T229M) abolished water transport activity. Co-expression of HvPIP1;2_24NC either with HvPIP2;4_12NC or with HvPIP2;4TM_12NC, in which the N- and C-terminal regions were replaced with the corresponding regions of HvPIP1;2, increased the water transport activity in oocytes. These data provided evidence that the HvPIP1;2 molecule has own water transport activity and an interaction with the middle part of the HvPIP2;4 protein (except for the N- and C-termini) is required for HvPIP1;2 functionality as a water channel. This molecular mechanism could be applied to other PIP1s and PIP2s in addition to the known mechanism that the terminal regions of some PIP2s lead some PIP1s to the plasma membrane.

    DOI： 10.1093/pcp/pcaa142

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    Other Link： http://academic.oup.com/pcp/article-pdf/62/1/92/36718180/pcaa142.pdf

  • Accession difference in leaf photosynthesis, root hydraulic conductance and gene expression of root aquaporins under salt stress in barley seedlings Reviewed International coauthorship International journal

    Asuka Kodama, Tamaki Watanabe, Makoto Yamaguchi, Ryohei Narita, Maki Katsuhara, Kazuhiro Sato, Taiichiro Ookawa, Tadashi Hirasawa

    Plant Production Science   24 ( 1 )   73 - 82   2021.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Informa UK Limited  

    DOI： 10.1080/1343943x.2020.1794915

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  • A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca2+-Sensitive HvPIP2;8 Na+ and K+ Conductance Reviewed International coauthorship International journal

    Sen Thi Huong Tran, Tomoaki Horie, Shahin Imran, Jiaen Qiu, Samantha McGaughey, Caitlin S. Byrt, Stephen D. Tyerman, Maki Katsuhara

    International Journal of Molecular Sciences   21 ( 19 )   7135 - 7135   2020.9

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, HvPIP2;8, facilitated cation transport when expressed in Xenopus laevis oocytes. HvPIP2;8-associated ion currents were detected with Na+ and K+, but not Cs+, Rb+, or Li+, and was inhibited by Ba2+, Ca2+, and Cd2+ and to a lesser extent Mg2+, which also interacted with Ca2+. Currents were reduced in the presence of K+, Cs+, Rb+, or Li+ relative to Na+ alone. Five HvPIP1 isoforms co-expressed with HvPIP2;8 inhibited the ion conductance relative to HvPIP2;8 alone but HvPIP1;3 and HvPIP1;4 with HvPIP2;8 maintained the ion conductance at a lower level. HvPIP2;8 water permeability was similar to that of a C-terminal phosphorylation mimic mutant HvPIP2;8 S285D, but HvPIP2;8 S285D showed a negative linear correlation between water permeability and ion conductance that was modified by a kinase inhibitor treatment. HvPIP2;8 transcript abundance increased in barley shoot tissues following salt treatments in a salt-tolerant cultivar Haruna-Nijo, but not in salt-sensitive I743. There is potential for HvPIP2;8 to be involved in barley salt-stress responses, and HvPIP2;8 could facilitate both water and Na+/K+ transport activity, depending on the phosphorylation status.

    DOI： 10.3390/ijms21197135

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  • Na+ Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na+ Concentration and Regulates Shoot Na+ Concentration Reviewed International journal

    Yuki Kawakami, Shahin Imran, Maki Katsuhara, Yuichi Tada

    International Journal of Molecular Sciences   21 ( 17 )   6100 - 6100   2020.8

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    We characterized an Na+ transporter SvHKT1;1 from a halophytic turf grass, Sporobolus virginicus. SvHKT1;1 mediated inward and outward Na+ transport in Xenopus laevis oocytes and did not complement K+ transporter-defective mutant yeast. SvHKT1;1 did not complement athkt1;1 mutant Arabidopsis, suggesting its distinguishable function from other typical HKT1 transporters. The transcript was abundant in the shoots compared with the roots in S. virginicus and was upregulated by severe salt stress (500 mM NaCl), but not by lower stress. SvHKT1;1-expressing Arabidopsis lines showed higher shoot Na+ concentrations and lower salt tolerance than wild type (WT) plants under nonstress and salt stress conditions and showed higher Na+ uptake rate in roots at the early stage of salt treatment. These results suggested that constitutive expression of SvHKT1;1 enhanced Na+ uptake in root epidermal cells, followed by increased Na+ transport to shoots, which led to reduced salt tolerance. However, Na+ concentrations in phloem sap of the SvHKT1;1 lines were higher than those in WT plants under salt stress. Based on this result, together with the induction of the SvHKT1;1 transcription under high salinity stress, it was suggested that SvHKT1;1 plays a role in preventing excess shoot Na+ accumulation in S. virginicus.

    DOI： 10.3390/ijms21176100

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  • Ectopic expression of a rice plasma membrane intrinsic protein (OsPIP1;3) promotes plant growth and water uptake Reviewed International coauthorship International journal

    Siyu Liu, Tatsuya Fukumoto, Patrizia Gena, Peng Feng, Qi Sun, Qiang Li, Tadashi Matsumoto, Toshiyuki Kaneko, Hang Zhang, Yao Zhang, Shihua Zhong, Weizhong Zeng, Maki Katsuhara, Yoshichika Kitagawa, Aoxue Wang, Giuseppe Calamita, Xiaodong Ding

    The Plant Journal   102 ( 4 )   779 - 796   2020.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/tpj.14662

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.14662

  • Expression and Ion Transport Activity of Rice OsHKT1;1 Variants Reviewed International journal

    Shahin Imran, Tomoaki Horie, Maki Katsuhara

    Plants   9 ( 1 )   16 - 16   2019.12

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    OsHKT1;1 in rice, belongs to the high-affinity K+ Transporter family, has been found to be involved in salt tolerance. OsHKT1;1 in japonica rice (Nipponbare) produces mRNA variants, but their functions remain elusive. In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA. Absolute quantification by qPCR revealed that accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots, while only V1 and V7 mRNAs are predominant in roots. Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously. Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution. OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein. Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions. These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist, and that OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL.

    DOI： 10.3390/plants9010016

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  • Dynamics of the contents and distribution of ABA, auxins and aquaporins in developing caryopses of an ABA-deficient barley mutant and its parental cultivar Reviewed

    Oksana A. Seldimirova, Guzel R. Kudoyarova, Maki Katsuhara, Ilshat R. Galin, Denis Yu. Zaitsev, Natalia N. Kruglova, Dmitry S. Veselov, Stanislav Yu. Veselov

    Seed Science Research   29 ( 4 )   261 - 269   2019.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cambridge University Press (CUP)  

    <title>Abstract</title>Dynamics of abscisic acid (ABA) and indole-3-acetic acid (IAA) contents were followed in developing barley caryopses of the ABA-deficient mutant AZ34 and its parental cultivar Steptoe. Distribution of these hormones and HvPIP2 aquaporins (AQPs) was studied with the help of immunohistochemical methods in the roots and coleorhiza of developing embryos. In Steptoe, maturation of the caryopsis was accompanied by vast accumulation of ABA, while this hormone accumulated more slowly in the caryopsis of AZ34 and its content was lower than in Steptoe. Accumulation of ABA was accompanied by a decline in IAA level in the developing caryopsis, the process being delayed in AZ34 in accordance with the slower accumulation of ABA. ABA accumulated to high levels in the coleorhiza cells of Steptoe, while the effect was absent in AZ34. The high level of ABA was likely to be important for maintaining the barrier function of the coleorhiza, preventing germination of seminal roots and enabling seed dormancy, while the absence of ABA accumulation in coleorhiza of AZ34 may be responsible for the initiation of root germination inside the caryopsis. The abundance of HvPIP2 AQPs in the seminal roots was higher at the beginning of maturation of Steptoe caryopsis and declined afterwards, while the levels of APQs increased later in AZ34 in accordance with the delay in ABA accumulation. These results suggest the importance of ABA accumulation in coleorhiza for preventing precocious growth of seminal roots, and suggest regulation of IAA and aquaporin levels by this hormone during maturation of embryos.

    DOI： 10.1017/s0960258519000229

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  • The Mechanism of SO2 -Induced Stomatal Closure Differs from O3 and CO2 Responses and Is Mediated by Non-Apoptotic Cell Death in Guard Cells. Reviewed International journal

    Ooi L, Matsuura T, Munemasa S, Murata Y, Katsuhara M, Hirayama T, Mori IC

    Plant, cell & environment   42 ( 2 )   437 - 447   2019.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism.

    DOI： 10.1111/pce.13406

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  • High-affinity K+ transporters from a halophyte, Sporobolus virginicus, mediate both K+ and Na+ transport in transgenic Arabidopsis, X. laevis oocytes, and yeast. Reviewed

    Tada Y, Endo C, Katsuhara M, Horie T, Shibasaka M, Nakahara Y, Kurusu T

    Plant & Cell Physiology   60 ( 1 )   176 - 187   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcy202

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  • Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein Reviewed

    Yuichi Tada, Ryuichi Kawano, Shiho Komatsubara, Hideki Nishimura, Maki Katsuhara, Soichi Ozaki, Shin Terashima, Kentaro Yano, Chisato Endo, Muneo Sato, Mami Okamoto, Yuji Sawada, Masami Yokota Hirai, Takamitsu Kurusu

    Plant Science   278   54 - 63   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.plantsci.2018.10.019

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  • Energy costs of salinity tolerance in crop plants Invited Reviewed

    Stephen D. Tyerman, Rana Munns, Wieland Fricke, Borjana Arsova, Bronwyn J. Barkla, Jayakumar Bose, Helen Bramley, Caitlin Byrt, Zhonghua Chen, Timothy D. Colmer, Tracey Cuin, David A. Day, Kylie J. Foster, Matthew Gilliham, Sam W. Henderson, Tomoaki Horie, Colin L. D. Jenkins, Brent N. Kaiser, Maki Katsuhara, Darren Plett, Stanley J. Miklavcic, Stuart J. Roy, Francisco Rubio, Sergey Shabala, Megan Shelden, Kathleen Soole, Nicolas L. Taylor, Mark Tester, Michelle Watt, Stefanie Wege, Lars H. Wegner, Zhengyu Wen

    New Phytologist   221 ( 1 )   25 - 29   2019.1

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    DOI： 10.1111/nph.15555

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  • A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na+ and K+ and Permeable to Na+ and K+ Non-Selectively Reviewed International journal

    Izumi Mori, Yuichi Nobukiyo, Yoshiki Nakahara, Mineo Shibasaka, Takuya Furuichi, Maki Katsuhara

    Plants   7 ( 3 )   61 - 61   2018.7

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    Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. Current was not observed in X. laevis oocytes injected with HvCNGC2-3 complementary RNA (cRNA) in a bathing solution containing either Na+ or K+ solely, even in the presence of 8-bromoadenosine 3′,5′-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3′,5′-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na+ and K+ in the bathing solution and in the presence of 10 µM 8Br-cAMP, but not 8Br-cGMP. Permeability ratios of HvCNGC2-3 to K+, Na+ and Cl− were determined as 1:0.63:0.03 according to reversal-potential analyses. Amino-acid replacement of the unique ion-selective motif of HvCNGC2-3, AQGL, with the canonical motif, GQGL, resulted in the abolition of the current. This study reports a unique two-ion-dependent activation characteristic of the barley CNGC, HvCNGC2-3.

    DOI： 10.3390/plants7030061

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  • T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress Reviewed

    Oda Y, Kobayashi NI, Tanoi K, Ma JF, Itou Y, Katsuhara M, Itou T, Horie T

    International Journal of Molecular Sciences   19 ( 1 )   2018.1

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    DOI： 10.3390/ijms19010235

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    Other Link： http://orcid.org/0000-0001-7545-2771

  • Identification of an H2O2permeable PIP aquaporin in barley and a serine residue promoting H2O2transport Reviewed

    Rhee J, Horie T, Sasano S, Nakahara Y, Katsuhara M

    Physiologia Plantarum   159 ( 1 )   120 - 128   2017.1

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    DOI： 10.1111/ppl.12508

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  • Exogenous application of abscisic acid (ABA) increases root and cell hydraulic conductivity and abundance of some aquaporin isoforms in the ABA-deficient barley mutant Az34 Reviewed

    Guzel Sharipova, Dmitriy Veselov, Guzel Kudoyarova, Wieland Fricke, Ian C. Dodd, Maki Katsuhara, Takuya Furuichi, Igor Ivanov, Stanislav Veselov

    Annals of Botany   118 ( 4 )   777 - 785   2016.10

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    DOI： 10.1093/aob/mcw117

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  • Genome-Wide Characterization of Major Intrinsic Proteins in Four Grass Plants and Their Non-Aqua Transport Selectivity Profiles with Comparative Perspective

    Abul Kalam Azad, Jahed Ahmed, Md. Asraful Alum, Md. Mahbub Hasan, Takahiro Ishikawa, Yoshihiro Sawa, Maki Katsuhara

    PLOS ONE   11 ( 6 )   e0157735   2016.6

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    DOI： 10.1371/journal.pone.0157735

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  • Phosphorus deprivation effects on water relations of Nicotiana tabacum plant via reducing plasma membrane permeability

    M. Mahdieh, A. Mostajeran, M. Katsuhara

    RUSSIAN JOURNAL OF PLANT PHYSIOLOGY   63 ( 1 )   54 - 61   2016.1

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    DOI： 10.1134/S102144371601012X

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  • OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress

    Kei Suzuki, Naoki Yamaji, Alex Costa, Eiji Okuma, Natsuko I. Kobayashi, Tatsuhiko Kashiwagi, Maki Katsuhara, Cun Wang, Keitaro Tanoi, Yoshiyuki Murata, Julian I. Schroeder, Jian Feng Ma, Tomoaki Horie

    BMC PLANT BIOLOGY   16 ( 1 )   22   2016.1

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    DOI： 10.1186/s12870-016-0709-4

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  • OsHKT2;2/1-mediated Na+ influx over K+ uptake in roots potentially increases toxic Na+ accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress

    Kei Suzuki, Alex Costa, Hideki Nakayama, Maki Katsuhara, Atsuhiko Shinmyo, Tomoaki Horie

    JOURNAL OF PLANT RESEARCH   129 ( 1 )   67 - 77   2016.1

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    DOI： 10.1007/s10265-015-0764-1

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  • Yeast functional screen to identify genes conferring salt stress tolerance in Salicornia europaea

    Yoshiki Nakahara, Shogo Sawabe, Kenta Kainuma, Maki Katsuhara, Mineo Shibasaka, Masanori Suzuki, Kosuke Yamamoto, Suguru Oguri, Hikaru Sakamoto

    FRONTIERS IN PLANT SCIENCE   6   920   2015.10

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    DOI： 10.3389/fpls.2015.00920

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  • Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds

    Shigeko Utsugi, Mineo Shibasaka, Masahiko Maekawa, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   56 ( 9 )   1831 - 1840   2015.9

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    DOI： 10.1093/pcp/pcv104

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  • Dynamic Regulation of the Root Hydraulic Conductivity of Barley Plants in Response to Salinity/Osmotic Stress

    Toshiyuki Kaneko, Tomoaki Horie, Yoshiki Nakahara, Nobuya Tsuji, Mineo Shibasaka, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   56 ( 5 )   875 - 882   2015.5

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    DOI： 10.1093/pcp/pcv013

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  • Osmotic stress decreases PIP aquaporin transcripts in barley roots but H2O2 is not involved in this process

    Maki Katsuhara, Nobuya Tsuji, Mineo Shibasaka, Sanjib Kumar Panda

    JOURNAL OF PLANT RESEARCH   127 ( 6 )   787 - 792   2014.11

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    DOI： 10.1007/s10265-014-0662-y

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  • Expression of root HvPIPs of barley seedling under chilling stress

    Rui-Jun Duan, Hui-Yan Xiong, Katsuhara Maki

    Zhiwu Shengli Xuebao/Plant Physiology Journal   50 ( 8 )   1203 - 1208   2014.8

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    DOI： 10.13592/j.cnki.ppj.2014.0242

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  • CO2 Transport by PIP2 Aquaporins of Barley

    Izumi C. Mori, Jiye Rhee, Mineo Shibasaka, Shizuka Sasano, Toshiyuki Kaneko, Tomoaki Horie, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   55 ( 2 )   251 - 257   2014.2

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    DOI： 10.1093/pcp/pcu003

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  • Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite

    Maki Katsuhara, Shizuka Sasano, Tomoaki Horie, Tadashi Matsumoto, Jiye Rhee, Mineo Shibasaka

    PLANT BIOTECHNOLOGY   31 ( 3 )   213 - U173   2014

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    DOI： 10.5511/plantbiotechnology.14.0421a

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  • The photosynthetic response of tobacco plants overexpressing ice plant aquaporin McMIPB to a soil water deficit and high vapor pressure deficit

    Miki Kawase, Yuko T. Hanba, Maki Katsuhara

    JOURNAL OF PLANT RESEARCH   126 ( 4 )   517 - 527   2013.7

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    DOI： 10.1007/s10265-013-0548-4

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  • Overexpression of Alternative Oxidase Gene Confers Aluminum Tolerance by Altering the Respiratory Capacity and the Response to Oxidative Stress in Tobacco Cells

    Sanjib Kumar Panda, Lingaraj Sahoo, Maki Katsuhara, Hideaki Matsumoto

    MOLECULAR BIOTECHNOLOGY   54 ( 2 )   551 - 563   2013.6

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    DOI： 10.1007/s12033-012-9595-7

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  • Water and CO2 permeability of SsAqpZ, the cyanobacterium Synechococcus sp. PCC7942 aquaporin

    Xiaodong Ding, Tadashi Matsumoto, Patrizia Gena, Chengwei Liu, Marialuisa Pellegrini-Calace, Shihua Zhong, Xiaoli Sun, Yanming Zhu, Maki Katsuhara, Ikuko Iwasaki, Yoshichika Kitagawa, Giuseppe Calamita

    Biology of the Cell   105 ( 3 )   118 - 128   2013.3

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    DOI： 10.1111/boc.201200057

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  • Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells

    NAKAYAMA Pulla K

    生物工学会誌 : seibutsu-kogaku kaishi   91 ( 2 )   85 - 85   2013.2

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  • Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination

    Chengwei Liu, Tatsuya Fukumoto, Tadashi Matsumoto, Patrizia Gena, Daniele Frascaria, Tomoyuki Kaneko, Maki Katsuhara, Shihua Zhong, Xiaoli Sun, Yanming Zhu, Ikuko Iwasaki, Xiaodong Ding, Giuseppe Calamita, Yoshichika Kitagawa

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   63   151 - 158   2013.2

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    DOI： 10.1016/j.plaphy.2012.11.018

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  • Salinity tolerance mechanisms in glycophytes: An overview with the central focus on rice plants. Reviewed

    Horie T, Karahara I, Katsuhara M

    Rice (New York, N.Y.)   5 ( 1 )   11 - 11   2012.12

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  • Influence of Low Air Humidity and Low Root Temperature on Water Uptake, Growth and Aquaporin Expression in Rice Plants

    Tsuneo Kuwagata, Junko Ishikawa-Sakurai, Hidehiro Hayashi, Kiyoshi Nagasuga, Keiko Fukushi, Arifa Ahamed, Katsuko Takasugi, Maki Katsuhara, Mari Murai-Hatano

    PLANT AND CELL PHYSIOLOGY   53 ( 8 )   1418 - 1431   2012.8

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    DOI： 10.1093/pcp/pcs087

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  • Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana

    Cortwa Hooijmaijers, Ji Ye Rhee, Kyung Jin Kwak, Gap Chae Chung, Tomoaki Horie, Maki Katsuhara, Hunseung Kang

    JOURNAL OF PLANT RESEARCH   125 ( 1 )   147 - 153   2012.1

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    DOI： 10.1007/s10265-011-0413-2

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  • Functional characterization of a novel plasma membrane intrinsic protein2 in barley

    Mineo Shibasaka, Sizuka Sasano, Sigeko Utsugi, Maki Katsuhara

    Plant Signaling and Behavior   7 ( 12 )   1642 - 1646   2012

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    DOI： 10.4161/psb.22294

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  • Effect of nutrient deficiencies on the water transport properties in figleaf gourd plants

    Ji Ye Rhee, Gap Chae Chung, Maki Katsuhara, Sung-Ju Ahn

    HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY   52 ( 6 )   629 - 634   2011.12

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    DOI： 10.1007/s13580-011-0046-3

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  • K+ Transport by the OsHKT2;4 Transporter from Rice with Atypical Na+ Transport Properties and Competition in Permeation of K+ over Mg2+ and Ca2+ Ions

    Tomoaki Horie, Dennis E. Brodsky, Alex Costa, Toshiyuki Kaneko, Fiorella Lo Schiavo, Maki Katsuhara, Julian I. Schroeder

    PLANT PHYSIOLOGY   156 ( 3 )   1493 - 1507   2011.7

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    DOI： 10.1104/pp.110.168047

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  • Mechanisms of Water Transport Mediated by PIP Aquaporins and Their Regulation Via Phosphorylation Events Under Salinity Stress in Barley Roots

    Tomoaki Horie, Toshiyuki Kaneko, Genki Sugimoto, Shizuka Sasano, Sanjib Kumar Panda, Mineo Shibasaka, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   52 ( 4 )   663 - 675   2011.4

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    DOI： 10.1093/pcp/pcr027

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  • Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells

    Tomoaki Horie, Mitsuo Sugawara, Tomoyuki Okada, Koichiro Taira, Pulla Kaothien-Nakayama, Maki Katsuhara, Atsuhiko Shinmyo, Hideki Nakayama

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   111 ( 3 )   346 - 356   2011.3

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  • Hormonal treatment of the bark of rubber trees (Hevea brasiliensis) increases latex yield through latex dilution in relation with the differential expression of two aquaporin genes

    Kessarin Tungngoen, Unchera Viboonjun, Panida Kongsawadworakul, Maki Katsuhara, Jean-Louis Julien, Soulaiman Sakr, Herve Chrestin, Jarunya Narangajavana

    JOURNAL OF PLANT PHYSIOLOGY   168 ( 3 )   253 - 262   2011.2

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    DOI： 10.1016/j.jplph.2010.06.009

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  • Abiotic stresses modulate expression of major intrinsic proteins in barley (Hordeum vulgare)

    Ayalew Ligaba, Maki Katsuhara, Mineo Shibasaka, Gemechis Djira

    COMPTES RENDUS BIOLOGIES   334 ( 2 )   127 - 139   2011.2

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    DOI： 10.1016/j.crvi.2010.11.005

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  • Early response in water relations influenced by NaCl reflects tolerance or sensitivity of barley plants to salinity stress via aquaporins

    Maki Katsuhara, Ji Ye Rhee, Genki Sugimoto, Gap Chae Chung

    SOIL SCIENCE AND PLANT NUTRITION   57 ( 1 )   50 - 60   2011.2

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    DOI： 10.1080/00380768.2010.541870

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  • Water and H2O2 Permeability of Aquaporins Isolated from Cucumber and Fig leaf Gourd

    Ji Ye Rhee, Maki Katsuhara, Hunseung Kang, Gap Chae Chung

    HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY   51 ( 3 )   167 - 172   2010.6

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  • Insights into the salt tolerance mechanism in barley (Hordeum vulgare) from comparisons of cultivars that differ in salt sensitivity

    Ayalew Ligaba, Maki Katsuhara

    JOURNAL OF PLANT RESEARCH   123 ( 1 )   105 - 118   2010.1

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    DOI： 10.1007/s10265-009-0272-2

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  • Differential Sodium and Potassium Transport Selectivities of the Rice OsHKT2;1 and OsHKT2;2 Transporters in Plant Cells

    Xuan Yao, Tomoaki Horie, Shaowu Xue, Ho-Yin Leung, Maki Katsuhara, Dennis E. Brodsky, Yan Wu, Julian I. Schroeder

    PLANT PHYSIOLOGY   152 ( 1 )   341 - 355   2010.1

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  • A bacterial biosensor for oxidative stress using the constitutively expressed redox-sensitive protein roGFP2. Reviewed International journal

    Arias-Barreiro CR, Okazaki K, Koutsaftis A, Inayat-Hussain SH, Tani A, Katsuhara M, Kimbara K, Mori IC

    Sensors (Basel, Switzerland)   10 ( 7 )   6290 - 6306   2010

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    A highly specific, high throughput-amenable bacterial biosensor for chemically induced cellular oxidation was developed using constitutively expressed redox-sensitive green fluorescent protein roGFP2 in E. coli (E. coli-roGFP2). Disulfide formation between two key cysteine residues of roGFP2 was assessed using a double-wavelength ratiometric approach. This study demonstrates that only a few minutes were required to detect oxidation using E. coli-roGFP2, in contrast to conventional bacterial oxidative stress sensors. Cellular oxidation induced by hydrogen peroxide, menadione, sodium selenite, zinc pyrithione, triphenyltin and naphthalene became detectable after 10 seconds and reached the maxima between 80 to 210 seconds, contrary to Cd(2+), Cu(2+), Pb(2+), Zn(2+) and sodium arsenite, which induced the oxidation maximum immediately. The lowest observable effect concentrations (in ppm) were determined as 1.0 × 10(-7) (arsenite), 1.0 × 10(-4) (naphthalene), 1.0 × 10(-4) (Cu(2+)), 3.8 × 10(-4) (H(2)O(2)), 1.0 × 10(-3) (Cd(2+)), 1.0 × 10(-3) (Zn(2+)), 1.0 × 10(-2) (menadione), 1.0 (triphenyltin), 1.56 (zinc pyrithione), 3.1 (selenite) and 6.3 (Pb(2+)), respectively. Heavy metal-induced oxidation showed unclear response patterns, whereas concentration-dependent sigmoid curves were observed for other compounds. In vivo GSH content and in vitro roGFP2 oxidation assays together with E. coli-roGFP2 results suggest that roGFP2 is sensitive to redox potential change and thiol modification induced by environmental stressors. Based on redox-sensitive technology, E. coli-roGFP2 provides a fast comprehensive detection system for toxicants that induce cellular oxidation.

    DOI： 10.3390/s100706290

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  • Regulation mechanism of water permeability under hypertonic stress in barley root

    Kaneko Toshiyuki, Horie Tomoaki, Shibasaka Mineo, Katsuhara Maki

    Plant and Cell Physiology Supplement   2010   0034 - 0034   2010

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    The salt stress is one of the representative abiotic stresses that decreases growth and productivity of crops plants. The root water permeability of three barley (Hordeum Vulgare) cultivars that show a difference in the salt sensitivity was measured under salinity stress using a pressure chamber. Water permeability of salt sensitive variety did not show any difference in response to salt stress, in contrast to significant reductions of water permeability found in more salt tolerant varieties. Root water permeability of tolerant cultivars exposed to 100 mM NaCl was found to be extremely down-regulated after 1h. Interestingly, however, water permeability was partially recovered after 4 h, which was followed by a re-down-regulation afterwards. Further measurements using various inhibitors indicated that at least protein phosphorylation and recycling plays an essential role in the regulation mechanism.

    DOI： 10.14841/jspp.2010.0.0034.0

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  • Involvement of HbPIP2;1 and HbTIP1;1 Aquaporins in Ethylene Stimulation of Latex Yield through Regulation of Water Exchanges between Inner Liber and Latex Cells in Hevea brasiliensis

    Kessarin Tungngoen, Panida Kongsawadworakul, Unchera Viboonjun, Maki Katsuhara, Nicole Brunel, Soulaiman Sakr, Jarunya Narangajavana, Herve Chrestin

    PLANT PHYSIOLOGY   151 ( 2 )   843 - 856   2009.10

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    DOI： 10.1104/pp.109.140228

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  • New production method for greening concrete blocks.

    Katsuhara M

    Environment Solution Technology   8 ( 3 )   32 - 36   2009

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  • Biomarkers of Green Roof Vegetation: Anthocyanin and Chlorophyll as Stress Marker Pigments for Plant Stresses of Rooftop Environments.

    Mori, I.C, Utsugi,S, Tanakamaru, S, Tani, A, Enomoto, T, Katsuhara, M

    Journal of Environmental Engineering and Management   19   21 - 27   2009

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  • Function and regulation of PIP-type water channels in roots of barley under salinity stress

    Horie Tomoaki, Kaneko Toshiyuki, Sugimoto Genki, Shibasaka Mineo, Katsuhara Maki

    Plant and Cell Physiology Supplement   2009   S0029 - S0029   2009

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    The plasma membrane intrinsic protein (PIP) forms the pore for water transport as a water channel in plant cells. We have studied the molecular mechanism of water transport in roots of barley (Hordeum Vulgare cv. Haruna-nijo). Quantitative RT-PCR analyses using roots of barley plants revealed significant reductions in the accumulation of 6 out of 10 HvPIP mRNA in response to 200 mM NaCl. Characterization of water permeability of barely roots using a pressure chamber showed that water permeability was extremely down-regulated in 200 mM NaCl condition. Furthermore, phosphorylation was found to regulate water transport activity. HvPIP1 proteins did not show apparent water transport activity when they were expressed alone in Xenopus laevis oocytes. However, co-expression of HvPIP1 proteins with HvPIP2 proteins was shown to enhance water transport activity of HvPIP2 proteins. The regulation mechanism of water transport via HvPIP proteins in barley roots under salinity stress will be discussed.

    DOI： 10.14841/jspp.2009.0.s0029.0

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  • Characterization of four plasma membrane aquaporins in tulip petals: A putative homolog is regulated by phosphorylation

    Abul Kalam Azad, Maki Katsuhara, Yoshihiro Sawa, Takahiro Ishikawa, Hitoshi Shibata

    PLANT AND CELL PHYSIOLOGY   49 ( 8 )   1196 - 1208   2008.8

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    DOI： 10.1093/pcp/pcn095

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  • Barley plasma membrane intrinsic proteins (PIP aquaporins) as water and CO(2) transporters

    Maki Katsuhara, Yuko T. Hanba

    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY   456 ( 4 )   687 - 691   2008.7

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    DOI： 10.1007/s00424-007-0434-9

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  • Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants

    Majid Mahdieh, Akbar Mostajeran, Tomoaki Horie, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   49 ( 5 )   801 - 813   2008.5

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    DOI： 10.1093/pcp/pcn054

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  • Presence of aquaporin and V-ATPase on the contractile vacuole of Amoeba proteus

    Eri Nishihara, Etsuo Yokota, Akira Tazaki, Hidefurni Orii, Maki Katsuhara, Kensuke Kataoka, Hisako Igarashi, Yoshinori Moriyama, Teruo Shimmen, Seiji Sonobe

    BIOLOGY OF THE CELL   100 ( 3 )   179 - 188   2008.3

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    DOI： 10.1042/BC20070091

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  • Female mating receptivity inhibited by injection of male-derived extracts in Callosobruchus chinensis

    Takashi Yamane, Yoshinobu Kimura, Maki Katsuhara, Takahisa Miyatake

    JOURNAL OF INSECT PHYSIOLOGY   54 ( 2 )   501 - 507   2008.2

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    DOI： 10.1016/j.jinsphys.2007.11.009

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  • Expanding roles of plant aquaporins in plasma membranes and cell organelles

    KATSUHARA M.

    Funct. Plant Biol.   35 ( 1 )   1 - 14   2008

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  • Barley root hydraulic conductivity and aquaporins expression in relation to salt tolerance

    Maki Katsuhara, Mineo Shibasaka

    SOIL SCIENCE AND PLANT NUTRITION   53 ( 4 )   466 - 470   2007.8

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    DOI： 10.1111/j.1747-0765.2007.00154.x

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  • An aluminum-activated citrate transporter in barley.

    Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

    Plant & cell physiology   48 ( 8 )   1081 - 91   2007.8

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    Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for (14)C-labeled citrate, but not for malate. Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

    DOI： 10.1093/pcp/pcm091

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  • An efflux transporter of silicon in rice

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

    NATURE   448 ( 7150 )   209 - U12   2007.7

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    DOI： 10.1038/nature05964

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  • Gene expression and functional analysis of a candidate gene related to Al-activated citrate transporter in barley Reviewed

    Furukawa Jun, Yamaji Naoki, Wang Hua, Katsuhara Maki, Sato Kazuhiro, Takeda Kazuyoshi, Ma Jian Feng

    Plant and Cell Physiology   48   S44   2007

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  • Characterization of silicon permeability of NIP in gramineous plants Reviewed

    Mitani Namiki, Yamaji Naoki, Katsuhara Maki, Ma Jian Feng

    PLANT AND CELL PHYSIOLOGY   48   S30   2007

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  • Synchronous changes of aquaporins in rice seedlings during early phase of salt-stress.

    Mineo Shibasaka, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   48   S162 - S162   2007

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  • Comparison of transpiration rates of naturalized and domestic Sedum species

    Izumi Mori, Takashi Enomoto, Maki Katsuhara

    PLANT AND CELL PHYSIOLOGY   48   S227 - S227   2007

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  • The BnALMT1 Protein That is an Aluminum-Activated Malate Transporter is Localized in the Plasma Membrane. International journal

    Ayalew Ligaba, Maki Katsuhara, Wataru Sakamoto, Hideaki Matsumoto

    Plant signaling & behavior   2 ( 4 )   1 - 2   2007

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    We have previously reported that Al-induces citrate and malate efflux from P-sufficient and P-deficient plants of rape (Brassica napus L.) and that P-deficiency alone could not induce this response. Further investigation showed that the transcript of two genes designated BnALMT1 and BnALMT2 is accumulated in roots by Al-treatment. Transgenic tobacco cells (Nicotiana tabacum) and Xenopus laevis oocytes expressing the BnALMT1 and BnALMT2 proteins released more malate than control cells in the presence of Al, indicating that the BnALMT genes encode an Al-activated malate transporter. The transgenic tobacco cells exposed to toxic level of Al grew better than control cells indicating that the genes can enhance Al-resistance of plant cells. In this study we showed the subcellular localization of BnALMT1 fused to the green fluoresce protein (GFP). The BnALMT1:: GFP construct was transiently expressed in protoplasts prepared from Arabidopsis leaves using the polyethylene glycol (PEG) method. The result showed that the BnALMT1 protein is localized in the plasma membrane. This provides further evidence that the BnALMT proteins facilitate the transport of malate across the plasma membrane (PM).

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  • Molecular mechanisms of water uptake and transport in plant roots: research progress with water channel aquaporins

    Katsuhara Maki

    Plant Root   1   22 - 26   2007

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    Aquaporins (water channels) are membrane proteins which facilitate the transport of water and low molecular weight compounds across biological membranes. The author and collaborators identified barley aquaporins and investigated the level of transcripts in roots, water transport activity, tissue localization, expression reduction by salt stress, and diurnal changes in the expression of a particular aquaporin. Over-expression of a barley aquaporin, HvPIP2;1, increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants. Aquaporin research is providing significant insights into the water relations of plant roots.

    DOI： 10.3117/plantroot.1.22

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  • Low Temperature and Aquaporins, a Molecular Mechanism of Water Transport

    KATSUHARA Maki, CHUNG Gap Chae, SAKURAI Junko, MURAI Mari, IZUMI Yohei, TSUMUKI Hisaaki

    Cryobiology and cryotechnology   53 ( 1 )   21 - 32   2007

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    Language：English   Publisher：Japanese Society for Cryobiology and Cryotechnology  

    Aquaporins are membrane proteins facilitating the membrane transport of water and low molecular weight compounds. Recent studies have indicated that aquaporins are involved in cellular tolerance mechanisms under low temperature stress in plants and insects. Critical roles of aquaporins in cold tolerance and the influence of H_2O_2 were suggested in the cucumber and figleaf gourd. Reduction of water uptake was observed in rice under chilling stress, in which the down-regulation of aquaporin expression and probable inactivation of aquaporins were involved. Glycerol and water transport by aquaporins was investigated, and revealed to be essential for freezing tolerance in insect cells.

    DOI： 10.20585/cryobolcryotechnol.53.1_21

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  • The BnALMT1 and BnALMT2 genes from rape encode aluminum-activated malate transporters that enhance the aluminum resistance of plant cells

    Ayalew Ligaba, Maki Katsuhara, Peter R. Ryan, Mineo Shibasaka, Hideaki Matsumoto

    PLANT PHYSIOLOGY   142 ( 3 )   1294 - 1303   2006.11

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    DOI： 10.1104/pp.106.085233

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  • A silicon transporter in rice

    JF Ma, K Tamai, N Yamaji, N Mitani, S Konishi, M Katsuhara, M Ishiguro, Y Murata, M Yano

    NATURE   440 ( 7084 )   688 - 691   2006.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/nature04590

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  • Hydraulic conductivity and aquaporins of cortical cells in gravitropically bending roots of Pisum sativum L.

    N Miyamoto, M Katsuhara, T Ookawa, K Kasamo, T Hirasawa

    PLANT PRODUCTION SCIENCE   8 ( 5 )   515 - 524   2005.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1626/pps.8.515

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  • Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis

    M Katsuhara, T Otsuka, B Ezaki

    PLANT SCIENCE   169 ( 2 )   369 - 373   2005.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.plantsci.2005.03.030

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  • A novel cyanobacterial SmtB/ArsR family repressor regulates the expression of a CPx-ATPase and a metallothionein in response to both Cu(I)/Ag(I) and Zn(II)/Cd(II)

    T Liu, S Nakashima, K Hirose, M Shibasaka, M Katsuhara, B Ezaki, DP Giedroc, K Kasamo

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 17 )   17810 - 17818   2004.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M310560200

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  • A wheat gene encoding an aluminum-activated malate transporter

    T Sasaki, Y Yamamoto, B Ezaki, M Katsuhara, SJ Ahn, PR Ryan, E Delhaize, H Matsumoto

    PLANT JOURNAL   37 ( 5 )   645 - 653   2004.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-313X.2003.01991.x

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  • Overexpression of the barley aquaporin HvPIP2;1 increases internal CO2 conductance and CO2 assimillation in the leaves of transgenic rice plants

    YT Hanba, M Shibasaka, Y Hayashi, T Hayakawa, K Kasamo, Terashima, I, M Katsuhara

    PLANT AND CELL PHYSIOLOGY   45 ( 5 )   521 - 529   2004

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pch070

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  • Changes in roots and salt sensitivity of rice plants over-expressing aquaporin

    M Katsuhara, K Koshio, M Shibasaka, Y Hayashi, T Hayakawa, K Kasamo

    PLANT AND CELL PHYSIOLOGY   45   S174 - S174   2004

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  • Expression of an aquaporin at night in relation to the growth and root water permeability in barley seedlings

    M Katsuhara, K Koshio, M Shibasaka, K Kasamo

    SOIL SCIENCE AND PLANT NUTRITION   49 ( 6 )   883 - 888   2003.12

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  • Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants

    M Katsuhara, K Koshio, M Shibasaka, Y Hayashi, T Hayakawa, K Kasamo

    PLANT AND CELL PHYSIOLOGY   44 ( 12 )   1378 - 1383   2003.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcg167

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  • A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

    T Liu, S Nakashima, K Hirose, Y Uemura, M Shibasaka, M Katsuhara, K Kasamo

    FEBS LETTERS   542 ( 1-3 )   159 - 163   2003.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0014-5793(03)00370-3

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  • Increase in CO2 permeability (diffusion conductance) in leaves of transgenic rice plant over-expressing barley aquaporin

    M Katsuhara, Y Hanba, M Shibasaka, Y Hayashi, T Hayakawa, K Kasamo

    PLANT AND CELL PHYSIOLOGY   44   S86 - S86   2003

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  • A novel histidine-rich CPx-ATPase from the filamentous cyanobacterium Oscillatoria brevis related to multiple-heavy-metal cotolerance

    L Tong, S Nakashima, M Shibasaka, M Katsuhara, K Kasamo

    JOURNAL OF BACTERIOLOGY   184 ( 18 )   5027 - 5035   2002.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/JB.184.18.5027-5035.2002

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  • Functional analysis of water channels in barley roots

    M Katsuhara, Y Akiyama, K Koshio, M Shibasaka, K Kasamo

    PLANT AND CELL PHYSIOLOGY   43 ( 8 )   885 - 893   2002.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcf102

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  • Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis

    B Ezaki, M Katsuhara, M Kawamura, H Matsumoto

    PLANT PHYSIOLOGY   127 ( 3 )   918 - 927   2001.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1104/pp.127.3.918

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  • Isolation of barley saLT gene: Its relation to salt tolerance and to hormonal regulation by abscisic acid and jasmonic acid

    M Katsuhara, M Yamada, K Kasamo

    SOIL SCIENCE AND PLANT NUTRITION   47 ( 1 )   187 - 193   2001.3

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  • Expression and stress-dependent induction of potassium channel transcripts in the common ice plant

    H Su, D Golldack, M Katsuhara, CS Zhao, HJ Bohnert

    PLANT PHYSIOLOGY   125 ( 2 )   604 - 614   2001.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1104/pp.125.2.604

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  • Identification and characterization of a putative heavy metal transport gene from a cyanobacterium Oscillatoria Brevis :

    Liu Tong, NAKASHIMA Susumu, SHIBASAKA Mineo, KATSUHARA Maki, KASAMO Kunihiro

    Plant and cell physiology   42   s208   2001

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    Language：English   Publisher：Japanese Society of Plant Physiologists  

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    Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=185274

  • In situ RNA hybridization using Technovit resin in Arabidopsis thaliana

    K Takechi, W Sakamoto, M Katsuhara, M Murata, F Motoyoshi

    PLANT MOLECULAR BIOLOGY REPORTER   17 ( 1 )   43 - 51   1999

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  • Intracellular pH and proton-transport in barley root cells under salt stress: In vivo 31P-NMR study

    Maki Katsuhara, Yoshiaki Yazaki, Katsuhiro Sakano, Toshio Kawasaki

    Plant and Cell Physiology   38 ( 2 )   155 - 160   1997

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    DOI： 10.1093/oxfordjournals.pcp.a029146

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  • Salt stress induced nuclear and DNA degradation in meristematic cells of barley roots

    Maki Katsuhara, Toshio Kawasaki

    Plant and Cell Physiology   37 ( 2 )   169 - 173   1996

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Plant Physiologists  

    DOI： 10.1093/oxfordjournals.pcp.a028928

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  • Distribution and Production of trans-Aconitic Acid in Barnyard Grass (Echinochloa crus-galli var. oryzicola) as Putative Antifeedant against Brown Planthoppers :

    KATSUHARA Maki, SAKANO Katsuhiro, SATO Mamoru, KAWAKITA Hiromu, KAWABE Susumu

    Plant and cell physiology   34 ( 2 )   251 - 254   1993

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    A quantitative analysis of organic acid in leaves of barnyard grass revealed that the contents of trans-aconitic acid were high, suggesting that this compound may act as an antifeedant against brown planthoppers. However, trans-aconitic acid could not be detected in the phloem sap which was considered to be the main nutrient source for brown planthoppers. trans-Aconitic acid was formed in vitro from cis-aconitic acid through the aconitate isomerase activity which was detected only in the leaf sheath, but not in the leaf blade.

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    Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=181825

  • Detection of mycoplasmalike organisms in the phloem sap collected from mulberry trees with dwarf disease.

    KAWAKITA HIROMU, KATSUHARA MAKI

    Journal of Insect Biotechnology and Sericology   62 ( 2 )   125 - 131   1993

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    Language：Japanese   Publisher：The Japanese Society of Sericultural Science  

    Mycoplasmalike organisms (MLOs), the pathogens of mulberry dwarf disease, were succesufully isolated by stylectomy method (cutting of insect stylets by YAG laser) using a homopterous insect, Ricania japonica. The MLOs were detectable in the phloem sap collected from diseased mulberry plants as fluorescent particles stained with 4, 6-diamidino-2-phenylindole (DAPI) under a fluorescence microscope but were not detected in the sap of healthy mulberry plants. The presence of MLOs in the phloem sap was also confirmed by electronmicroscopy.<br>On the other hand, two kinds of leafhoppers, vectors of mulberry dwarf MLOs, and other some tested hemipterous insects could not used for this laser stylectomy. Scanning-electronmicroscopical observation showed that the stylets of these leafhoppers which were not suitable for the stylectomy method were pushed out or broken after laser cutting, whwereas stylets of Ricania japonica remained firmly in the tissues.

    DOI： 10.11416/kontyushigen1930.62.125

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    Other Link： http://agriknowledge.affrc.go.jp/RN/2010510238

  • Cytoplasmic Alkalization and Cytoplasmic Streaming Induced by Light and Histidine in Leaf Cells of Egeria densa: in vivo 31P-NMR study

    Yoshito Tominaga, Kazuyuki Kuchitsu, Maki Katsuhara, Masashi Tazawa, Shigetoh Miyachi

    Plant and Cell Physiology   32 ( 2 )   261 - 268   1991.3

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press ({OUP})  

    DOI： 10.1093/oxfordjournals.pcp.a078072

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  • Patch-clamp study on ion channels in the tonoplast of nitellopsis obtusa

    Maki Katsuhara, Tetsuro Mimura, Masashi Tazawa

    Plant and Cell Physiology   32 ( 2 )   179 - 184   1991

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    DOI： 10.1093/oxfordjournals.pcp.a078062

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  • Mechanism of calcium‐dependent salt tolerance in cells of Nitellopsis obtusa: role of intracellular adenine nucleotides

    M. KATSUHARA, M. TAZAWA

    Plant, Cell &amp; Environment   13 ( 2 )   179 - 184   1990

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-3040.1990.tb01290.x

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  • ATP-regulated ion channels in the plasma membrane of a characeae alga, Nitellopsis obtusa

    Maki Katsuhara, Tetsuro Mimura, Masashi Tazawa

    Plant Physiology   93 ( 1 )   343 - 346   1990

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Society of Plant Biologists  

    DOI： 10.1104/pp.93.1.343

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  • Patch-clamp study on a Ca2+-regulated K+ channel in the tonoplast of the brackish characeae lamprothamnium succinctum

    Maki Katsuhara, Tetsuro Mimura, Masashi Tazawa

    Plant and Cell Physiology   30 ( 4 )   549 - 555   1989

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    DOI： 10.1093/oxfordjournals.pcp.a077774

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  • Rapid cytoplasmic alkalization and dynamics of intracellular compartmentation of inorganic phosphate during adaptation against salt stress in a halotolerant unicellular green alga dunaliella tertiolecta:31P-nuclear magnetic resonance study Reviewed

    Kuchitsu, K., Katsuhara, M., Miyachi, S.

    Plant and Cell Physiology   30 ( 3 )   407 - 414   1989

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  • ATP IS ESSENTIAL FOR CALCIUM-INDUCED SALT TOLERANCE IN NITELLOPSIS-OBTUSA

    M KATSUHARA, M TAZAWA

    PROTOPLASMA   138 ( 2-3 )   190 - 192   1987

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Books

  • 植物栄養学（第3版）

    馬, 建鋒, 信濃, 卓郎, 高野, 順平（ Role： Joint author ,  水ポテンシャルと水の吸収 pp37-42）

    文永堂出版  2024.7  （ ISBN:9784830041457 ）

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    Total pages：xx, 318p   Language：Japanese

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  • 根っこってなんだろう?

    且原真木, 松波麻耶（ Role： Joint author ,  根っこはどうやって水を吸収するの）

    文研出版  2023.8  （ ISBN:9784580825970 ）

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    Total pages：47p   Responsible for pages：10-11p   Language：Japanese Book type：Textbook, survey, introduction

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  • バイオスティミュラントハンドブック : 植物の生理活性プロセスから資材開発、適用事例まで

    日本バイオスティミュラント協議会, 山内, 靖雄, 須藤, 修, 和田, 哲夫（ Role： Contributor ,  第１編第2章第4節 水ストレスが植物に与える影響と植物の応答）

    エヌ・ティー・エス  2022.4  （ ISBN:9784860437695 ）

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    Total pages：iii, xiii, 460, xiip, 図版vip   Language：Japanese

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  • 植物学の百科事典(共著)

    丸善  2016 

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  • 植物栄養学第２版（共著）

    文永堂  2010 

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  • 水とからだの事典（共著）

    朝倉書店  2008 

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  • みずみずしい体のしくみ：水の通り道「アクアポリン」の働きと病気」

    クバプロ  2005 

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  • 植物の膜輸送システム ポンプ・トランスポーター・チャンネル研究の新展開

    秀潤社  2003 

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  • Molecular aspects of potassium and water transport, and their bearing under salt stress

    Plant Nutrition for sustainable food production and environment 

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  • パッチクランプによるチャンネルの解析

    「植物の細胞を観る実験プロトコール」 

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  • Molecular aspects of potassium and water transport, and their bearing under salt stress

    Plant Nutrition for sustainable food production and environment 

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MISC

  • Controls of the water transport activities of Arabidopsis tonoplast intrinsic proteins 3, AtTIP3s and the effects on seed development and germination

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   64th   2023

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  • Analysis of CO2 transport activity of plasma membrane-associated aquaporins of tomato

    光本晏理, 宇都木繁子, 土屋善幸, 且原真木, 森泉

    日本植物生理学会年会(Web)   64th   2023

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  • Controls of the water transport activities of Arabidopsis tonoplast intrinsic proteins 3, AtTIP3s

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   63rd   2022

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  • 植物のアクアポリン

    且原 真木

    脳神経内科   94 ( 5 )   635 - 642   2021.5

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    Authorship：Corresponding author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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  • オオムギ種子における液胞膜型アクアポリン(HvTIPs)の役割

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   60th   2019

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

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  • 種子で発現するオオムギ液胞膜型アクアポリン(HvTIPs)のタンパク質間相互作用

    宇都木繁子, 柴坂三根夫, 且原真木

    日本植物生理学会年会(Web)   59th   2018

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  • オオムギ種子における液胞膜型アクアポリンの水輸送活性の調節

    宇都木繁子, 柴坂三根夫, 且原真木

    日本植物生理学会年会要旨集   55th   2014

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  • 種子特異的に発現するオオムギTIP3;1による水輸送活性の調節

    宇都木繁子, 篠野静香, 柴坂三根夫, 且原真木

    日本植物生理学会年会要旨集   54th   2013

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  • 原形質膜型アクアポリンPIP1とPIP2の共発現による活性抑制

    柴坂三根夫, 篠野静香, 宇都木繁子, 且原真木

    日本植物生理学会年会要旨集   54th   2013

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  • オオムギMIPファミリーの解析

    宇都木繁子, 柴坂三根夫, 且原真木, 前川雅彦

    育種学研究   13   2011

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  • Varietal differences in physiological responses to salt stress in barley seedings

    YAMAGUCHI Makoto, WATANABE Tamaki, KATSUHARA Maki, TAKEDA Kazuyoshi, OOKAWA Taiichiro, HIRASAWA Tadashi

    79   182 - 183   2010.9

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  • 岡山大学資源生物科学研究所における屋上緑化による建物冷却効果

    且原真木, 田中丸重美, 森泉, 谷明生, 宇都木繁子, 榎本敬, 米谷俊彦

    環境制御   31 ( 1 )   21 - 25   2009

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  • 新しい緑化ブロック作成技術

    且原真木

    環境浄化技術   8 ( 3 )   32 - 36   2009

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  • コムギ種子特異的な液胞型アクアポリン(TIP)の解析

    宇都木繁子, 且原真木, 前川雅彦

    育種学研究   11   2009

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  • 105 Varietal differences in root hydraulic conductivity and the rate of leaf photosyntesis under salt stress conditions in barley seedlings

    Watanabe Tamaki, Sato Kosuke, Katsuhara Maki, Ookawa Taiichiro, Takeda Kazuyoshi, Hirasawa Tadashi

    Japanese Journal of Crop Science   77 ( 2 )   210 - 211   2008.9

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  • 9-6 オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析(9.植物の無機栄養,2007年度東京大会)

    古川 純, 山地 直樹, 王 華, 三谷 奈見季, 村田 佳子, 佐藤 和広, 且原 真木, 武田 和義, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 53 )   64 - 64   2007.8

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  • B201 アズキゾウムシにおけるオス由来のメスの交尾抑制物質の生化学的分析

    山根 隆史, 宮竹 貴久, 木村 吉伸, 村田 芳行, 且原 真木, 中島 修平

    日本応用動物昆虫学会大会講演要旨   ( 51 )   20 - 20   2007.3

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  • オオムギのアルミニウム活性型クエン酸輸送体候補遺伝子の発現ならびに機能解析

    古川純, 山地直樹, 王華, 且原真木, 佐藤和広, 武田和義, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007

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  • イネ科NIPのケイ酸輸送特性の解析

    三谷奈見季, 山地直樹, 且原真木, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007

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  • 9-66 イネケイ酸吸収遺伝子Lsi1の輸送特性の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    三谷 奈見季, 且原 真木, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 52 )   72 - 72   2006.9

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  • オオムギアクアポリンHvPIP2;1の過剰発現による形質転換イネの葉内CO2拡散コンダクタンスと光合成速度の増加

    半場祐子, 柴坂三根夫, 林泰行, 早川孝彦, 笠毛邦宏, 寺島一郎, 且原真木

    日本植物生理学会年会要旨集   47th   2006

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  • アクアポリンの多様性と多面性:耐塩性や光合成との関係

    且原真木, 柴坂三根夫, 半場祐子

    日本植物生理学会年会要旨集   46th   2005

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  • 植物の根に関する諸問題-水透過性の分子機構：根における水チャネル・アクアポリンの機能-

    且原真木

    農業および園芸   79:600-605 ( 5 )   600 - 605   2004

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  • A Molecular mechanisms of water absorption and water transport : recent progress in study on water channels

    KATSUHARA Maki

    Root Research   13:15-20 ( 1 )   15 - 20   2004

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    Discovery of aquaporin (water channel) genes have facilitated investigations into the molecular mechanisms of cellular water permeability and transport. We isolated genes of plasma-membrane type aquaporins in barley roots and demonstrated the water transport activity, tissue localization, and diurnal change in expression of a particular aquaporin, HvPIP2;1, in barley. Over-expression of HvPIP2;1 increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants. Furthermore, we noticed the increase in internal CO2 conductance of transgenic plants. This finding indicated that HvPIP2;1 can facilitate permeation of a CO2 as well as water molecule.

    DOI： 10.3117/rootres.13.15

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  • 9-4 コムギのアルミニウム活性化型リンゴ酸トランスポーター遺伝子の機能解析 : 対立遺伝子間の機能相違(9.植物の無機栄養)

    佐々木 孝行, 山本 洋子, 且原 真木, 松本 英明

    日本土壌肥料学会講演要旨集   ( 49 )   66 - 66   2003.8

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  • コムギのA1耐性機構 : リンゴ酸分泌とそのトランスポーター遺伝子

    佐々木 孝行, 山本 洋子, 且原 真木, 大沢 裕樹, 松本 秀明

    根の研究 = Root research   12 ( 2 )   71 - 71   2003.6

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  • A gene encoding an aluminum-activated malate transporter segregates with aluminum tolerance in wheat

    T Sasaki, Y Yamamoto, B Ezaki, M Katsuhara, PR Ryan, E Delhaize, H Matsumoto

    PLANT AND CELL PHYSIOLOGY   44   S84 - S84   2003

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  • Studies on salt stress-tolerant mechanism in barley.1. Analysis of genes expressed specifically under salt stress in salt stress-tolerant barley

    SUGIMOTO M, KATSUHARA M, OAKADA Y, SATO K, ITO K, TAKEDA K

    育種学研究 = Breeding research   4 ( 1 )   20 - 20   2002.3

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  • 9-3 アルミニウム耐性小麦に特異的な遺伝子の機能解析(9.植物の無機栄養)

    佐々木 孝行, 江崎 文一, 山本 洋子, 且原 真木, 松本 英明

    日本土壌肥料学会講演要旨集   ( 48 )   46 - 46   2002.3

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  • Cloning and characterization of a gene expressed in aluminum-tolerant wheat

    T Sasaki, Y Yamamoto, B Ezaki, M Katsuhara, H Matsumoto

    PLANT AND CELL PHYSIOLOGY   43   S51 - S51   2002

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  • 研究室・研究所めぐり（２２）岡山大学・資源生物科学研究所

    且原真木

    遺伝   54 ( 2 )   67 - 69   2000

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Presentations

  • OsPIP2;4イオンチャネルアクアポリンOsPIP2;4の分子機構と生理機能

    且原真木, Sen Tran, 小野峻太郎, 大西亜耶, 三戸祐之輔, 堀江智明

    日本植物学会第88回大会  2024.9.16 

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    Event date： 2024.9.14 - 2024.9.16

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  • 根圏のH2O2・鉄・有機物を制御して環境修復・地球温暖化阻止・有機物利用を進める

    我妻忠雄, 且原真木, 土屋善幸, 田原恒, Toan Nguyen Sy, 程為国, 俵谷圭太郎, 塩野義人, 本間幸

    日本土壌肥料学会2024年度福岡大会  2024.9.3 

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    Event date： 2024.9.3 - 2024.9.5

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • シャジクモ（Chara braunii）細胞膜Na＋共役型リン酸輸送体分子の機能解析

    三村徹郎, 藤原ひとみ, 村西直樹, 大西美輪, 西山智明, 菅野里美, 石崎公庸, 深城英弘, 坂山英俊, Robert Reid, 且原真木

    第65回日本植物生理学会  2024.3.19 

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    Event date： 2024.3.17 - 2024.3.19

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • イネのイオンチャネルアクアポリンicAQPの同定と生理機能の解析

    三戸祐之輔, Sen Tran, 大西亜耶, 小野峻太郎, 且原真木, 堀江智明

    第65回日本植物生理学会  2024.3.17 

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    Event date： 2024.3.17 - 2024.3.19

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  • Plant molecular physiology of salt stress and salt tolerance mechanisms Invited International conference

    Maki Katsuhara

    The 6th IBOC International Biology Conference 2023  2023.10.25 

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    Event date： 2023.10.25

    Language：English   Presentation type：Oral presentation (invited, special)  

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  • Na ion and K ion transport mechanism of ion channel aquaporins. International conference

    Ono S, Katsuhara M

    Taiwan Japan Plant Biology 2023  2023.10.14 

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    Event date： 2023.10.13 - 2023.10.15

    Language：English   Presentation type：Poster presentation  

    Venue：Taipei  

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  • Carbon dioxide transport by Arabidopsis PIP2 aquaporins. International conference

    Tania SS, Utsugi S, Tsuchiya Y, Sasano S, Katsuhara M, Mori I

    Taiwan Japan Plant Biology 2023  2023.10.14 

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    Event date： 2023.10.13 - 2023.10.15

    Language：English   Presentation type：Poster presentation  

    Venue：Taipei  

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  • Identification, characterization, and molecular mechanisms of ion-channel aquaporins in plants. International coauthorship International conference

    Katsuhara M, Tran THS, Ono S, Horie Tomoaki

    Taiwan Japan Plant Biology 2023  2023.10.14 

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    Event date： 2023.10.13 - 2023.10.15

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Taipei  

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  • Salt-tolerant Pokkali Rice OsHKT1;1 Variants: Expression and Ion Transport Activity. International conference

    Imran S, Ono S, Tomoaki H, Katsuhara M

    Taiwan Japan Plant Biology 2023  2023.10.14 

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    Event date： 2023.10.13 - 2023.10.15

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    Venue：Taipei  

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  • 根圏でのH2O2とFeによるFenton 反応を制御して、有機質資源利用と地球温暖化阻止を進める

    我妻忠雄, Toan Nguyen Syl, 程 為国, 田原 恒, 且原真木, 土屋善幸, 俵谷圭太郎

    日本土壌肥料学会2023年度愛媛大会  2023.9.13 

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    Event date： 2023.9.12 - 2023.9.14

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：松山  

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  • 植物のイオン輸送性アクアポリン

    且原真木, Tran THS, 小野修太朗, 堀江智明

    第17回トランスポーター研究会  2023.5.27 

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    Event date： 2023.5.27

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    Venue：名古屋  

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  • 耐塩性オオムギの根水透過性 (Lpr) 下方制御に関わるアクアポリンHvPIP2;1のPhos-tag® SDS-PAGEを使ったリン酸化解

    大西亜耶, 且原真木

    第17回トランスポーター研究会  2023.5.27 

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    Event date： 2023.5.27

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    Venue：名古屋  

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  • イオン透過性アクアポリンの Na+イオンおよび K+イオン輸送メカニズム

    小野峻太郎, 且原真木

    第64回日本植物生理学会  2023.3.13 

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    Event date： 2023.3.13 - 2023.3.17

    Language：Japanese   Presentation type：Poster presentation  

    Venue：仙台  

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  • サボテンの高温・乾燥耐性を支えるアクアポリンの組織分布と生理機能の解明

    佐藤良介, 榊原光, 水口慎太郎, 御堂育子, 鈴木孝征, 堀部貴紀, 柘植尚志, 且原真木, 前島正義

    第64回日本植物生理学会  2023.3.13 

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    Event date： 2023.3.13 - 2023.3.17

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    Venue：仙台  

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  • トマト原形質膜局在型アクアポリンの CO2 輸送活性の解析

    光本晏理, 宇都木繁子, 土屋善幸, 且原真木, 森泉

    第64回日本植物生理学会  2023.3.13 

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    Event date： 2023.3.13 - 2023.3.17

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  • シロイヌナズナ液胞膜型アクアポリン AtTIP3 サブファミリーの水輸送活性調節と種子発達と発芽への影響

    宇都木繁子, 且原真木

    第64回日本植物生理学会  2023.3.13 

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    Event date： 2023.3.13 - 2023.3.17

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    Venue：仙台  

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  • 塩ストレス初期段階における耐塩性オオムギK305の根水透過性 (Lpr) 下方制御とオオムギアクアポリンHvPIP2;1のリン酸化の解析

    大西亜耶, 且原真木

    第56回根研究集会（福岡）（オンライン）  2022.9.17 

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    Event date： 2022.9.17 - 2022.9.18

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  • イネにおけるイオン輸送性アクアポリンの同定と機能解析

    且原真木, Sen Tran, 堀江智明

    日本植物学会第86回大会（京都）  2022.9.17 

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    Event date： 2022.9.15 - 2022.9.19

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  • 鉄との協同による植物の新規な根圏改変機能 - 根圏フェントン反応による有機環境の改変・ストレスの回避 ①

    我妻忠雄, 田原恒, 且原真木, 土屋善幸, 俵谷圭太郎

    日本土壌肥料学会2022年度大会  2022.9 

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    Event date： 2022.9.13 - 2022.9.15

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • サボテンの高い環境耐性に対するアクアポリンの生理的役割の解明

    佐藤 良介, 鈴木孝征, 朝比奈 雅志, 堀部 貴紀, 柘植尚志, 且原真木, 前島 正義

    第63回日本植物生理学会（つくば）（オンライン）  2022.3 

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    Event date： 2022.3.22 - 2022.3.24

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  • シロイヌナズナ液胞膜型アクアポリンAtTIP3ファミリーの水輸送活性の制御

    宇都木 繁子, 且原 真木

    第63回日本植物生理学会（つくば）（オンライン）  2022.3 

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    Event date： 2022.3.22 - 2022.3.24

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  • シロイヌナズナ液胞膜型アクアポリンAtTIP3 の水輸送活性をコントロールするC末端領域の解析

    宇都木 繁子, 且原 真木

    第62回日本植物生理学会（島根）（オンライン）  2021.3 

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    Event date： 2021.3.14 - 2021.3.16

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  • イネアクアポリンOsPIP2;4 の発現量と根水透過性（Lpr）の相関性

    大西 亜耶, 且原 真木

    第62回日本植物生理学会（島根）（オンライン）  2021.3 

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    Event date： 2021.3.14 - 2021.3.16

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  • Ca2+-sensitive and non-selective Na+/K+ channel activity of a barley aquaporin HvPIP2;8.

    Tran S, Horie T, Imran S, Qiu J, McGaughey S, Byrt SC, Tyerman SD, Katsuhara M

    2021.3 

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    Event date： 2021.3.14 - 2021.3.16

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  • シロイヌナズナの液胞膜型アクアポリンTIP2;2 の機能解析

    山成 由佳, 中原 由揮, 藤本 緋菜, 本廣 夕佳, 桑形 恒男, 半場 祐子, 且原 真木, 奈良 久美

    第62回日本植物生理学会（島根）（オンライン）  2021.3 

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    Event date： 2021.3.14 - 2021.3.16

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  • PIP1型アクアポリン水輸送機能の新しい活性化メカニズム

    柴坂三根夫, 堀江智明, 且原真木

    日本植物学会第84回大会（名古屋）（オンライン）  2020.9 

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    Event date： 2020.9.19 - 2020.9.21

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  • 塩ストレス初期段階でのオオムギアクアポリンHvPIP2;1 のリン酸修飾による根水透過性 (Lpr) の下方制御機構

    大西亜耶, 且原真木

    第61回日本植物生理学会（大阪）（オンライン）  2020.3 

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    Event date： 2020.3.19 - 2020.3.21

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Ca2+-sensitive and non-selective Na+/K+ channel activity in a barley aquaporin HvPIP2;8

    Sen Thi Huong Tran, Maki Katsuhara, Tomoaki Horie

    2020.3 

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    Event date： 2020.3.19 - 2020.3.21

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  • イネのTILLING 変異系統を用いたOsHKT1;4 Na+ 輸送体の塩ストレス下における生理機能の解明

    河村敏貴, 小林奈通子, グエンタンハオ, 石川亮, 且原真木, 田野井慶太朗, 松坂弘明, 熊丸敏博, センテナックヘルベ, ベリーアナリエナ, 堀江智明

    第61回日本植物生理学会（大阪）（オンライン）  2020.3 

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    Event date： 2020.3.19 - 2020.3.21

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  • シロイヌナズナ液胞膜型アクアポリン (AtTIPs) の水輸送活性の解析

    宇都木繁子, 且原真木

    第61回日本植物生理学会（大阪）（オンライン）  2020.3 

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    Event date： 2020.3.19 - 2020.3.21

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  • Expression and Ion Transport Activity of Salt Tolerant Pokkali Rice OsHKT1;1 Variants.

    Shahin Imran, Maki Katsuhara, Tomoaki Horie

    2020.3 

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    Event date： 2020.3.19 - 2020.3.21

    Language：English   Presentation type：Oral presentation (general)  

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  • デンジソウの就眠運動リズムにおけるアクアポリンの発現解析

    田中福人, 且原真木, 富岡憲治

    第26回日本時間生物学会学術大会（金沢）  2019.10 

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    Event date： 2019.10.12 - 2019.10.13

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • シロイヌナズナ液胞膜型アクアポリンAtTIP2;2の過酸化水素透過性の検討

    山成由佳子, 中原由揮, 且原真木, 奈良久美

    日本植物学会第83回大会（仙台）  2019.9 

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    Event date： 2019.9.15 - 2019.9.17

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  • 時計因子ELF3は胚軸における水輸送調節にどうのように関連しているか？

    藤田知美, 奥村綾子, 土平絢子, 前島正義, 且原真木, 奈良久美

    日本植物学会第83回大会（仙台）  2019.9 

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    Event date： 2019.9.15 - 2019.9.17

    Language：Japanese   Presentation type：Poster presentation  

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  • 塩生植物アッケシソウから単離した新規ペプチドの遺伝子（SeNN43）は植物の耐塩性を向上させ、根の細胞を膨張させる

    坂本光, 開沼健太, 北村蒼門, 中原由揮, 且原真木, 小栗秀

    第60回日本植物生理学会（名古屋）  2019.3 

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    Event date： 2019.3.13 - 2019.3.15

    Language：Japanese   Presentation type：Poster presentation  

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  • オオムギ種子における液胞膜型アクアポリン（HvTIPs）の役割

    宇都木繁子, 且原真木

    第60回日本植物生理学会（名古屋）  2019.3 

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    Event date： 2019.3.13 - 2019.3.15

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Electrophysiological Analysis of Rice OsHKT1;1 variants

    Shahin Imran, Maki Katsuhara, Tomoaki Horie

    2019.3 

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    Event date： 2019.3.13 - 2019.3.15

    Language：English   Presentation type：Oral presentation (general)  

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  • 耐塩性の異なるオオムギ品種の塩ストレス初期応答

    大西亜耶, 且原真木

    第60回日本植物生理学会（名古屋）  2019.3 

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    Event date： 2019.3.13 - 2019.3.15

    Language：Japanese   Presentation type：Poster presentation  

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  • オオムギの根水透過性制御にかかわる遺伝因子の探求

    大西亜耶, 且原真木

    第49回根研究集会（盛岡）  2018.10 

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    Event date： 2018.10.27 - 2018.10.28

    Language：Japanese   Presentation type：Poster presentation  

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  • Na+/K+透過性をもつアクアポリン：イネOsPIP2;4およびオオムギHvPIP2;8の解析

    且原真木, 堀江智明

    第49回根研究集会（盛岡）  2018.10 

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    Event date： 2018.10.27 - 2018.10.28

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • シロイヌナズナの液胞膜型アクアポリンAtTIP2;2とAtTIP2;3の水透過性

    山成由佳子, 中原由揮, 且原真木, 奈良久美

    日本植物学会第82回大会（広島）  2018.9 

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    Event date： 2018.9.14 - 2018.9.16

    Language：Japanese   Presentation type：Poster presentation  

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  • シャジクモ細胞膜リン酸輸送体の分子機能解析

    三村徹郎, 大西美輪, 村西直樹, 藤原ひとみ, 石崎公康, 深城英弘, 西山智明, 坂山英俊, Rob, J.Reid, 且原真木

    日本植物学会第82回大会（広島）  2018.9 

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    Event date： 2018.9.14 - 2018.9.16

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Regulation of water transport and Na+ influx. Invited

    Katsuhara, M

    The 22nd New Phytologist Workshop ‘Energy costs of salinity tolerance in crop plants’ (Adelaide, Australia)  2018.4 

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    Event date： 2018.4.10 - 2018.4.12

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • CO2輸送体候補OsTIP2;2の細胞内局在

    中原由揮, 柴坂三根夫, 谷口洋二郎, 且原真木

    第59回日本植物生理学会（札幌）  2018.3 

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    Event date： 2018.3.28 - 2018.3.30

    Language：Japanese   Presentation type：Poster presentation  

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  • CO2輸送体候補アクアポリンOsTIP2;2の同定と機能解析

    中原由揮, 柴坂三根夫, 森泉, 谷口純一郎, 堀江智明, 金子智之, 且原真木

    第59回日本植物生理学会（札幌）  2018.3 

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    Event date： 2018.3.28 - 2018.3.30

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • イネアクアポリンOsPIP2;4 の発現量と根水透過性(Lpr)の相関性

    大西 亜耶, 且原 真木

    第52回根研究集会（熊本）(オンライン)  2020.11.21 

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    Language：Japanese   Presentation type：Poster presentation  

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  • Regulation in root hydraulic conductivity (Lpr) and PIP aquaporins under salt stress.

    Katsuhara, M

    Aquaporin solutions - Innovation conference.  2020.11.10 

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    Language：English   Presentation type：Oral presentation (invited, special)  

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  • Regulation of water transport and sodium influx in plant cells Invited

    Katsuhara, M

    Jilin University College of Plant Science Symposium  2019.10.17 

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    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • 塩ストレス下でのナトリウム流入と水輸送の制御 Invited

    且原真木

    第34回資源植物科学シンポジウム・第10回植物ストレス科学研究シンポジウム  2018.3.5 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 内膜系におけるアクアポリンの輸送基質と機能

    第19回植物オルガネラワークショップ  2017 

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  • ダイコン野生種ハマダイコンにおける海岸生-琵琶湖陸封生の間での耐塩性分化

    日本植物学会第81回大会  2017 

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  • 種子におけるオオムギ液胞膜型アクアポリン（HvTIPs）の相互作用と水輸送活性

    第58回日本植物生理学会年会  2017 

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  • Identification of the CO2 transporters from among the plant aquaporins.

    Taiwan-Japan Plant Biology 2017  2017 

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  • ハマダイコンにおけるセシウム蓄積特性の解析

    日本植物学会第81回大会  2017 

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  • A barley HvCNGC2-3, is activated by cAMP and the co-presence of external Na+ and K+, and permeates Na+ and K+ non-selectively.

    Taiwan-Japan Plant Biology 2017  2017 

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  • シロイヌナズナ概日時計変異体elf3 の根の水透過性

    日本植物学会第80回大会  2016 

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  • 海浜植物ハマエンドウのエコタイプ間に見られる環境応答能力の違い

    日本植物分類学会第15回大会  2016 

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  • 地球環境問題と形質転換作物：避けて通れない課題

    倉敷地方新農業経営者クラブ連絡協議会セミナー  2016 

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  • Barley and rice aquaporins transporting hydrogenperoxide.

    第57回日本植物生理学会年会  2016 

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  • A Salicornia europaea gene (SeNN43) encodes a novel short peptide that can improve salt tolerance in plants.

    第57回日本植物生理学会年会  2016 

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  • オオムギ原形質膜型アクアポリンPIP2 ファミリーの機能と根における発現プロファイル

    日本植物学会第80回大会  2016 

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  • 沈水植物ヤナギモにおける水輸送タンパク質PIPの働き

    日本植物生理学会2014年度年会  2014 

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  • Plasma membrane intrinsic protein1 (PIP1) is a modulator of PIP2 water channel.

    日本植物生理学会2014年度年会  2014 

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  • CO2輸送体スクリーニング法の開発

    日本植物生理学会2014年度年会  2014 

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  • 琵琶湖湖岸と海浜に生育するハマエンドウにおける環境ストレスへの適応的な応答

    日本植物生理学会2014年度年会  2014 

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  • イネOsHAK2およびOsHAK5輸送体の機能解析

    日本植物生理学会2014年度年会  2014 

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  • CO2 permeability of PIP2 aquaporins.

    日本植物生理学会2014年度年会  2014 

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  • The effect of aquaporin McMIPB and RsPIP2 on leaf photosynthesis of tobacco and eucalyptus plants.

    日本植物生理学会2014年度年会  2014 

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  • Preliminary dissection of CO2 permeability of a cyanobacterium.

    日本植物生理学会2014年度年会  2014 

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  • 過酸化水素輸送性アクアポリンの同定と機能/発現解析

    日本植物学会第78回大会  2014 

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  • 琵琶湖に隔離されたハマエンドウにおける耐塩性低下の検証

    植物分類学会第13回大会  2014 

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  • Plant aquaporin: A molecular regulator in plant water relations.

    The 1st Okayama University and King Faisal University's Workshop on Environmental and Life Science.  2014 

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  • 淡水域に隔離されたハマエンドウの耐塩性低下に関する比較研究

    日本植物学会第78回大会  2014 

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  • 塩生植物アッケシソウの新規耐塩性遺伝子の機能スクリーニング

    第54日本植物生理学会年会  2013 

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  • イネの水利用、成長、アクアポリン発現におよぼす低湿度と低地温の影響

    第54日本植物生理学会年会  2013 

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  • 種子特異的に発現するオオムギTIP3;1による水輸送活性の調節

    第54日本植物生理学会年会  2013 

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  • 琵琶湖に陸封されたハマエンドウと海浜集団の間に生じた光合成特性の分化

    第54日本植物生理学会年会  2013 

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  • 琵琶湖に隔離されたハマエンドウと海浜集団の間におけるABAを介した光合成特性の分化

    日本植物学会第77回大会  2013 

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  • それではいかに放射性物質に対応するか：植物科学からの取り組み

    吉備の国クラスター・エコ環境グループ講演会  2013 

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  • 水輸送とCO2輸送の分子基盤：アクアポリン

    日本植物学会第77回大会シンポジウム  2013 

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  • 原形質膜型アクアポリンPIP1とPIP2の共発現による活性抑制

    第54日本植物生理学会年会  2013 

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  • アクアポリンのCO2透過性

    第54日本植物生理学会年会  2013 

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  • イネアクアポリンOsPIPの発現と根水透過性：浸透圧ストレスの影響および形質転換での解析

    第54日本植物生理学会年会  2013 

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  • Root hydraulic conductivity and PIP aquaporins of barely and rice in response to salinity/osmotic stress.

    植物膜生物学国際学会2013  2013 

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  • 重金属イオンによる植物アクアポリン阻害作用についての検討

    第54日本植物生理学会年会  2013 

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  • Effect of low root-temperature on water use, growth and aquaporin expression in rice plants under various air-humidity conditions.

    植物膜生物学国際学会2013  2013 

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  • The functional analysis of Cyclic-Nucleotide Gated Cation Channels (CNGCs) in barley.

    植物膜生物学国際学会2013  2013 

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  • The photosynthetic response of tobacco and eucalyptus plants overexpressing aquaporins.

    植物膜生物学国際学会2013  2013 

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  • Gating of plasma membrane intrinsic protein1 via interaction with plasma membrane intrinsic protein2.

    植物膜生物学国際学会2013  2013 

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  • 植物細胞の水チャネルの調節

    神谷宣郎先生生誕百周年記念シンポジウム  2013 

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  • オオムギにおけるNa輸送性CNGCチャネルの機能解析

    日本土壌肥料学会2012年度鳥取大会  2012 

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  • Osmotic-induced reduction of root hydraulic conductivity is essential as an early response in slat/osomotic tolerance

    ゴードン科学会議 植物の塩と水ストレス  2012 

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  • イネとオオムギの亜ヒ酸輸送性NIP型アクアポリン

    日本植物学会第76回大会  2012 

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  • 水生植物ヤナギモにおける水輸送タンパク質PIPの解析

    日本植物学会第76回大会  2012 

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  • The Third Type of PIP Aquaporins.

    日本植物生理学会2012年度年会  2012 

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  • Regulation of root hydraulic conductivity in gramineous plants under osmotic stress.

    日本植物生理学会2012年度年会  2012 

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  • オオムギとイネの根水透過性制御と浸透圧ストレス応答

    第36回根研究集会  2012 

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  • アクアポリンのCO2透過性

    第3回植物アクアポリン研究会  2011 

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  • 浸透圧ストレス環境下におけるオオムギの水利用機能研究

    第3回植物アクアポリン研究会  2011 

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  • 亜ヒ酸輸送活性を持つイネおよびオオムギのアクアポリン

    第3回植物アクアポリン研究会  2011 

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  • オオムギの根水透過性とアクアポリンの浸透圧ストレス応答

    第3回植物アクアポリン研究会  2011 

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  • 原形質膜局在型アクアポリンの共発現による活性化の分子機構

    第3回植物アクアポリン研究会  2011 

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  • 浸透圧ストレス環境下におけるイネ科作物の水利用機能研究

    第35回根研究集会  2011 

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  • Dynamic regulation of root hydraulic conductivity and aquaporins under salt stress.

    The JSRR's 20th Anniversary Symposium  2011 

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  • 緑化ブロックと屋上緑化の新展開

    第二回かんきょうひろば全体交流会  2011 

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  • Dynamic regulation of root hydraulic conductivity and aquaporins under salt stress.

    International symposium, Strategies of Plants against Global Environmental Change.  2011 

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  • 形質膜アクアポリンPIP1の水チャンネルはPIP2によって開けられる

    日本植物生理学会2011年度年会  2011 

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  • Detection of transport activity in barley and rice aquaporins with newly developed yeast system.

    日本植物生理学会2011年度年会  2011 

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  • Hydrogen peroxide permeability of aquaporin in rice, barley and Arabidopsis

    第3回植物アクアポリン研究会  2011 

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  • オオムギPIP1型と2型アクアポリンのへテロマー形成の分子機構

    日本植物生理学会2010年度年会  2010 

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  • 植物における水輸送の分子基盤・アクアポリン：動物との共通点と相違点

    3部局合同セミナー  2010 

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  • 原形質膜局在型アクアポリンPIP1とPIP2の共発現による活性化メカニズムの解析

    日本植物生理学会2010年度年会  2010 

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  • 緑化ブロックの開発

    第４回産学官民コミュニティー全国大会 in おかやま  2010 

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  • Regulation of Root Water Permeability in Barley under Salt Stress.

    ゴードン科学会議（植物の塩およびお水ストレス）  2010 

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  • Cooling effect on buildings by the roof greening at Institute of Plant Science and Resources, Okayama University.

    第５回ジョモケニアッタ農工大学科学・技術；産業会議  2010 

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  • Studies on water channel activity of a PIP1 aquaporin and its interaction with PIP2 aquaporins.

    第１５回国際植物膜生物学ワークショップ  2010 

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  • Water transport in plants under stresses: from molecules to whole plant.

    第５回ジョモケニアッタ農工大学科学・技術；産業会議  2010 

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  • 高浸透圧ストレス下におけるオオムギ根の水輸送活性制御機構の解明

    日本植物生理学会2010年度年会  2010 

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  • Aquaporin - molecular mechanism of water transport in plants: Common and different characteristics between animals and plants.

    2010 

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  • 水と植物の２つの視点から 見えること 考えること

    第四回岡山大学いちょう並木研究サロン  2009 

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  • 原形質膜局在型アクアポリンPIP1とPIP2の共発現による活性化メカニズムの解析

    日本植物生理学会2009年度年会  2009 

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  • オオムギアクアポリンの機能と塩ストレス応答

    日本植物生理学会2009年度年会  2009 

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  • オオムギ根の水輸送活性は塩ストレス下において翻訳後修飾によって抑制されている

    日本植物生理学会2009年度年会  2009 

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  • Varietal differences in the rates of root growth and leaf photosynthesis, hydraulic conductivity and expressions of aquaporins in barley seedlings under salt stress conditions.

    PlantBiology2009  2009 

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  • 花と果実におけるアクアポリンの発現・調節と機能

    日本植物生理学会2009年度年会  2009 

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  • アクアポリンが光合成機能に果たす役割の定量的評価

    日本生態学会近畿地区会2009年第２回例会  2009 

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  • 高等植物の光合成と水チャネル

    第55回日本生態学会福岡大会  2008 

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  • マメゾウムシ二種におけるオスによるメスの交尾抑制の変異と種間交尾がメスの再交尾に与える影響

    第55回日本生態学会福岡大会  2008 

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  • Xenopus oocyteとPichia pastorisで発現させたチューリップ花弁由来アクアポリンの水輸送活性：ホモログTgPIP2;2はリン酸化によって制御される

    日本植物生理学会2008年度年会  2008 

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  • Water transport in rice and Graminean plants: from whole plant to aquaporins.

    Beijing Botanical Society Workshop  2008 

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  • 草本植物の茎葉では機械的ストレスを水分移動によって緩和している

    日本植物生理学会2008年度年会  2008 

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  • オオムギ原形質膜型アクアポリンの発現制御と水輸送特性

    日本植物生理学会2008年度年会  2008 

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  • マメゾウムシ２種におけるオスの交尾抑制物質の生化学的分析

    第５２回日本応用動物昆虫学会大会  2008 

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  • セイヨウナシアクアポリンPcPIP2;2のリン酸化による水輸送活性調節

    日本植物生理学会2008年度年会  2008 

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  • タバコアクアポリンの機能・発現と根の水透過性の制御

    第２８回根研究会  2008 

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  • 屋上緑化の現状と展望：資源生物科学研究所の取り組みと研究開発

    吉備の国クラスター・エコ環境グループ講演会  2008 

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  • 植物と水と私たち

    第53回大原孫三郎總一郎記念講演会  2008 

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  • オオムギ根における水透過性測定のためのpressure chamber作成と測定法の確立

    第２８回根研究会  2008 

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  • プロジェクト概要と現在の活動について

    岡山大学資源生物科学研究所屋上緑化プロジェクト公開報告・見学会  2008 

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  • コンクリート製緑化ブロック

    平成２０年度第１回ＴＬＯシーズ説明会  2008 

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  • Transcriptional Regulations and Water Transport Activity of PIP-type Aquaporins in Roots of Barley.

    Gordon Research Conference - Salt & Water Stress In Plants  2008 

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  • Phosphorylational regulation of plasma membrane aquaporin, PIP2, and its physiological roles in fruit and flower.

    FESPB2008  2008 

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  • 塩ストレス条件下におけるオオムギ幼植物の根の水伝道度と光合成速度の品種間差

    日本作物学会第226回講演会  2008 

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  • PIP aquaporins and water relations in barley roots under salt stress.

    Gordon Research Conference ? Salt & Water Stress In Plants  2008 

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  • セイヨウナシのアクアポリンPIP2のリン酸化による活性調節と果実日肥大におけるリン酸化状態の変化

    園芸学会平成２０年度秋季大会  2008 

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  • オオムギ原形質膜型アクアポリンの発現と機能および塩ストレスと重金属の影響の解析

    日本植物学会第７２回大会  2008 

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  • 塩ストレス条件下におけるオオムギ根の水透過性

    第２９回根研究会  2008 

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  • 耐塩性の異なるオオムギ２品種の根における原形質膜型アクアポリンの発現

    第２９回根研究会  2008 

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  • シロイヌナズナの浸透圧ストレス誘導性糖トランスポーターERD6の機能解析

    第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会  2008 

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  • オオムギ液胞膜型アクアポリン遺伝子の環境ストレスによる発現調節

    日本植物生理学会2007年度年会（愛媛） 2007年3月28日-30日  2007 

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  • Aquaporins mediate the transports of essential molecules in plants growing in various environments.

    5th International Conference of Aquaporin  2007 

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  • The effect of aquaporin HvPIP2;1 and McMIPA on leaf photosynthesis for rice and tobacco plants.

    5th International Conference of Aquaporin (Nara, Japan) 2007年7月13日-16日  2007 

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  • オオムギ原形質膜型アクアポリン遺伝子の同定と塩ストレスによる発現制御

    日本植物学会第７１回大会  2007 

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  • Multi-functional plant aquaporins mediating transports of water and essential molecules for plant life.

    Special Lectures by Professors in Sister Research (Agricultural Plant Stress Research Center, Chonnam National University, Korea)  2007 

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  • 緑化ブロックと建物緑化新技術の開発

    岡山大学知恵の見本市2007  2007 

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  • オオムギ原形質膜型アクアポリン遺伝子の同定と塩ストレスによる発現制御

    第２７回根研究会  2007 

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  • アズキゾウムシにおけるオス由来のメス交尾抑制物質の生化学的分析

    第51回日本応用動物昆虫学会  2007 

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  • 植物のアクアポリンの多彩な役割とその制御

    第９回植物生体膜シンポジウム「植物の生理機能と生理学的構造の役割」（  2007 

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  • イネ科NIPのケイ酸輸送特性の解析

    日本植物生理学会2007年度年会  2007 

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  • オオムギのアルミニウム活性型クエン酸輸送体候補遺伝子の発現ならびに機能解析

    日本植物生理学会2007年度年会  2007 

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  • 塩ストレスの初期段階におけるイネアクアポリン発現の同調的変動

    日本植物生理学会2007年度年会（愛媛） 2007年3月28日-30日  2007 

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  • 在来および帰化マンネングサ属の水利用効率の比較

    日本植物生理学会2007年度年会  2007 

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  • アクアポリン分子の基質輸送に伴う分子骨格構造の変動

    日本植物生理学会2006年度年会  2006 

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  • Cloning and functional analysis of an aluminium-induced malate transporter gene from rape (Brassica napus).

    日本植物生理学会2006年度年会  2006 

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  • 水輸送の分子機構・アクアポリンと低温

    第５２回低温生物工学会セミナー  2006 

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  • 水環境とオオムギの遺伝子発現制御

    日本植物生理学会2006年度年会  2006 

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  • A molecular investigation of an aluminium-induced malate secretion from rape (Brassica napus L.).

    PlantBiology2006  2006 

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  • Salt and osmotic stress regulates differentially plasma-membrane-type aquaporin gene expression in salt tolerant and salt sensitive barley.

    Gordon Research Conference – Salt & Water Stress In Plants  2006 

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  • Cloning and Functional Analysis of Plasma Membrane Aquaporin Subfamilies of Tulip Petal.

    PlantBiology2006  2006 

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  • ヒ素と重金属に応答する植物モニター系の構築

    岡山大学・学内COE「資源生物を用いた地球環境のモニター系の構築と環境保全への応用」国際ワークショップ  2006 

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  • アクアポリンの水ストレスへの応答と多彩な機能

    東北農業研究セミナー「生命を支える分子システム－植物の水吸収をになう「アクアポリン」研究の最前線」  2006 

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  • 岡山県南部に適化した薄層省管理緑化の新システムの開発

    大学発新事業創出促進事業「技術シーズ発信会」  2005 

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  • オオムギのCDPK遺伝子および液胞膜型CAX遺伝子の同定と機能解析

    第69回日本植物学会  2005 

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  • Osmotic stress regulates differentially aquaporin genes expression in salt tolerant and salt sensitive barley (Hordeum vulgare).

    4th Plant Genomic European Meetings  2005 

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  • 水チャネル・MIP遺伝子ファミリーの多様な機能

    第１回ムギ類研究会  2005 

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  • オオムギの液胞膜型Cation/H+ exchanger (CAX)

    第８回植物生体膜シンポジウム  2005 

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  • アクアポリンの多様性と多面性：耐塩性や光合成との関係 （シンポジウム アクアポリンの多様性：構造、機能、局在）

    日本植物生理学会2005年度年会シンポジウム  2005 

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  • イネゲノム中の全てのPIPファミリー遺伝子

    日本植物生理学会2005年度年会  2005 

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  • Transcriptional regulation of plasma membrane intrinsic protein (PIP) aquaporins in barley (Hordeum vulgare ,Haruna-nijo) by heavy metals.

    10th International Congress SABRAO  2005 

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  • Functional and molecular analysis of the barley Ca2+/H+ antiporter.

    Plant Biology2005  2005 

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  • オオムギ根の水輸送とアクアポリンファミリー

    第21回根研究集会  2004 

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  • 屈性を示している根の細胞の水吸収

    第１回植物アクアポリン研究討論会  2004 

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  • オオムギ水チャネルとその塩ストレス応答についての解析

    第１回植物アクアポリン研究討論会  2004 

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  • オオムギアクアポリンHvPIP2;1を過剰発現させた形質転換イネ葉での二酸化炭素拡散コンダクタンスと光合成速度の上昇

    第１回植物アクアポリン研究討論会  2004 

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  • Regulation of malate efflux in wheat root apex under aluminum stress: role of protein phosphorylation and ALMT1 gene.

    The 1st International Symposium "Life and Environmental Science in Future"  2004 

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  • 水チャンネル過剰発現イネにおける根の形質と塩ストレス応答性の変化

    日本植物生理学会2004年度年会  2004 

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  • 植物細胞におけるイオンと水：その生理と輸送機構の動物細胞との共通点と相違点

    第４７回日本腎臓学会学術総会  2004 

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  • オオムギ根のアクアポリンと水輸送、および塩ストレスとの関係

    第68回日本植物学会  2004 

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  • Aquaporins under salt stress

    Gordon Research Conference "Salt and Water Stress in Plants"  2004 

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  • オオムギにおけるストレス応答性遺伝子MAPKおよびCAXの単離と機能解析

    第21回根研究集会  2004 

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  • グルタチオン包合体の液胞膜透過機構に関するABCトランスポーター遺伝子

    第68回日本植物学会  2004 

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  • 植物のアクアポリンの働き

    第19回「大学と科学」公開シンポジウム みずみずしい体のしくみ：水の通り道“アクアポリン”の働きと病気  2004 

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  • タバコ由来のグルタチオン-Ｓ-トランスフェラーゼ遺伝子（parB）の導入によって活性酸素除去系を強化した形質転換シロイヌナズの塩ストレスに対する応答

    日本植物学会第６７回大会  2003 

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  • オオムギ幼根の水透過性に関連する水チャネルの夜間における発現

    第１９回根研究集会  2003 

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  • オオムギ水チャンネルは二酸化炭素を透過する-形質転換イネ葉での二酸化炭素拡散コンダクタンスの上昇-

    関西光合成研究会  2003 

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  • オオムギの水輸送と塩ストレス応答

    第１９回根研究集会  2003 

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  • 重金属イオン輸送体、CPx-ATPase遺伝子(Bxa1)の単離と特性解析

    日本植物生理学会2003年度年会  2003 

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  • コムギにおけるアルミニウム活性化型リンゴ酸トランスポーター遺伝子のアルミニウム耐性への関与

    日本植物生理学会2003年度年会  2003 

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  • コムギのAl耐性機構－リンゴ酸分泌とそのトランスポーター遺伝子－

    第１８回根研究集会  2003 

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  • オオムギ水チャンネル遺伝子を過剰発現させた形質転換体イネ緑葉での二酸化炭素透過性（拡散コンダクタンス）の上昇

    日本植物生理学会2003年度年会  2003 

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  • コムギのアルミニウム活性化型リンゴ酸トランスポーター遺伝子の機能解析：対立遺伝子間の機能相違

    日本土壌肥料学会２００３年度神奈川大会  2003 

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  • Increase in leaf CO2 conductance and decrease in salt tolerance in transgenic rice plant over-expressing barley aquaporin.

    Plant Biology 2003  2003 

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  • アルミニウム耐性小麦におけるリンゴ酸放出機構の分子生物学的解析

    第6回植物生体膜シンポジウム  2002 

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  • 塩ストレス抵抗性オオムギに関する研究１． 塩ストレスで特異的に発現する遺伝子の解析

    日本農芸化学会２００２年度大会  2002 

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  • 植物水チャンネルの遺伝子と機能

    第6回植物生体膜シンポジウム  2002 

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  • アルミニウム耐性小麦に特異的な遺伝子の機能解析

    日本土壌肥料学会2002年度名古屋大会  2002 

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  • オオムギの水チャネルHvPIP2;1の塩ストレスおよび乾燥ストレス下における機能的発現と日周変化

    日本植物生理学会2002年度年会  2002 

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  • かび臭物質生産ラン藻Oscillatoria brevisの多種金属耐性に関わる重金属イオン輸送体に関する研究

    日本水処理生物学会第３９回大会  2002 

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  • かび臭物質生産ラン藻Oscillatoria brevisの重金属耐性に関する分子生物学的研究

    日本水処理生物学会第３９回大会  2002 

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  • ラン藻Oscillatoria brevisからの重金属輸送遺伝子の同定と解析

    日本植物生理学会2001年度年会  2001 

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  • オオムギ根から単離した水チャンネル遺伝子の機能発現

    日本植物生理学会2001年度年会  2001 

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  • ラン藻Oscillatoria brevisのP-型重金属イオン輸送ATPaseの研究

    日本植物学会第65回大会  2001 

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  • オオムギの水チャンネル遺伝子bpw1の局在性と発現の日周変化

    日本植物生理学会2001年度年会  2001 

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  • Water channels in barley roots.

    The 6th International Society of Root Research Symposium  2001 

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  • ラン藻Oscillatoria brevisのメタロチオネイン遺伝子の同定

    日本植物学会第65回大会  2001 

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  • 塩ストレス誘導性細胞死と生体膜

    植物生体膜シンポジウム  2000 

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  • Apoptosis-like cell death under salt stress.

    Gordon Research Conference "Cellular Basisi of Adaptation to Salt and Water Stress in Plants"  2000 

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  • 植物の水チャンネルの構造と機能発現

    第17回資源生物科学シンポジウム  2000 

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  • Cell death and stress response in plants

    International symposium on "Stress responses in biological systems"  1999 

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  • 塩ストレス誘導性細胞死の初期過程における細胞膜インテグリティーの研究

    日本植物生理学会1999年度年会  1999 

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  • オオムギからの水チャンネル遺伝子の単離と塩ストレス下における発現の解析

    日本植物生理学会1999年度年会 1999年3月  1999 

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  • 耐塩性の異なるオオムギ3品種におけるsalT様遺伝子の発現制御

    日本植物学会第63回大会  1999 

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Works

  • 都市緑化おかやまフェア2009

    2009

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Awards

  • 第５回村川技術奨励賞

    2010  

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    Country：Japan

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  • 日本作物学会論文賞

    2007  

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    Country：Japan

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  • 日本植物生理学会論文賞

    2006  

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    Country：Japan

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Research Projects

  • 水とミネラル（カリウム、ナトリウム）を同一分子で取り込む植物の輸送体：その分子メカニズム解明と利用応用のための基盤研究

    2024.10 - 2025.09

    公益財団法人両備檉園記念財団  研究助成金 

    且原真木

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    Authorship：Principal investigator 

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  • Remediation of the agricultural environments using the plant species with roots containing high H2O2 in the presence of woody plant organics-Fe complex via rhizosphere Fenton reaction

    Grant number：21K05869  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    我妻 忠雄, 且原 真木, 俵谷 圭太郎, 田原 恒

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    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    キク科植物根のみが過酸化水素(H2O2)を大量に放出し、周辺鉄（Fe）との根圏Fenton反応でメタン、アトラジンを分解できることを見出した(Wagatsuma et al. 2021, 2022)。以上を踏まえ実験し、以下の結果を得た：（１）キュウリ、オオムギ、マリーゴールド（キク科）幼植物根を、H2DCF(細胞内活性酸素に蛍光)とBESH2O2(細胞内H2O2に蛍光)で、根アポプラスト中のH2O2をKI + starch溶液で、それぞれ染色した。その結果、オオムギはH2O2が不検出、キュウリ、マリーゴールドは細胞内に高濃度のH2O2を持つが、マリーゴールドのみが根外にH2O2を放出することが解った。（２）高塩環境で進化発生したキク科植物根から大量に放出されるH2O2は、根圏土壌catalaseでO2とH2Oに速やかに分解され、塩類土壌根圏に不足するO2の補給、塩類濃度の希釈、土壌緻密度の降下などによって塩ストレス回避に貢献すると推察した。（３）間伐材phenolicsからのFenton反応用の高性能鉄資材の創製のために、まずユーカリ葉を用いた。70 % 含水アセトン抽出液中の水溶液にFeCl3を反応させ、Phenolics-Fe錯体を得た。この凍結乾燥粉末の水溶性画分には、有機物Homologueとして一般に用いられているメチレンブルー(MB)をH2O2存在下で分解するFenton活性が、不溶性画分にはFenton活性とMB吸着能が認められ、これら能力には反復性があった。今後の方向性は、以下の通りである：①より高濃度のH2O2を放出するキク科植物を探索する。②H2O2供給土壌のcatalase活性の変化を明らかにすること。③今回創製したPhenolics-Fe錯体よりも、より強力な活性を持つ鉄資材の創製に取り組むこと。④土壌腐植、各種有機質肥料・資材などに対するFenton反応による小分子化の実態を明らかにすること。⑤以上により、Fenton活性による根圏有機環境の制御によって、有害有機環境を修復するとともに、高塩や低リンなどのストレスを回避させ得るかを解明すること。

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  • デュアル輸送機能を示す新型アクアポリンの分子機構と生理機能の解明

    Grant number：20K06708  2020.04 - 2024.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    且原 真木, 堀江 智明

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    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    本研究では水輸送機能と同時にナトリウム輸送機能も合わせ持つ、すなわち２つの異なる輸送機能をデュアルで示す新型アクアポリンを対象として、分子生理学および電気生理学的手法によって機能特性を解明すること、また免疫組織化学や形質転換体を用いて生理機能を明らかにすることを目指している。
    本年度はイネの原形質膜型アクアポリンPIP11種をアフリカツメガエル卵母細胞に発現させて二電極電圧クランプ法を適用して解析した。その結果イネ原形質膜型アクアポリンの一つOsPIP2;4がK+やNa+を透過させることを見出した。OsPIP2;4は葉より根での発現が高く、間接蛍光抗体法により主として外皮で発現していることがわかった。OsPIP2;4は、細胞外Ca2+濃度が低いときは一価カチオンに対する輸送活性を示した（透過性の順序はK+ > Na+ > Rb+ ～ Cs+ > Li+）が、高Ca2+条件ではイオン輸送活性は阻害された（IC50=92μM）。OsPIP1型アクアポリンと共発現させると、OsPIP2;4のイオン輸送性は阻害された。人為的にアミノ酸を置換した変異OsPIP2;4の解析から、水輸送とイオン輸送は分子内で別々の機構によって制御されていると考えられた。すなわち、水を輸送するが一価カチオンを輸送しない変異OsPIP2;4分子や、逆に水を輸送できないが一価カチオンを輸送する変異OsPIP2;4分子を得ることができた。またOsPIP2;4による過剰発現体と抑制体のイネ植物体を使って塩ストレス下でのNaやKの蓄積量を調べた結果、野生型イネと比べて過剰発現体では150 mM NaClの塩ストレスによってNaの蓄積が増加する一方で抑制体ではNaの蓄積が減少することが明らかになった。

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  • A Novel Therapeutic Strategy for Macular Edema Targeting Aquaporins.

    Grant number：17K11450  2017.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Shiraga Fumio

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    Grant amount：\4680000 （ Direct expense: \3600000 、 Indirect expense：\1080000 ）

    Macular edema is a common condition in diabetic retinopathy, retinal vein occlusion, age-related macular degeneration, and macular epiretinal membranes. Excessive changes in water and osmolality impair the cells that make up the macula, resulting in decreased vision. In this study, we focused on retinal pigment epithelial cells, which are responsible for water transport in the macula, and aquaporins, which are water transport proteins in cells, and investigated the effects of various stresses on cells in macular diseases on the expression of aquaporins in retinal pigment epithelial cells. The results showed that the expression of aquaporin-1 was significantly decreased by extension stress on the cells. On the other hand, there were no significant changes in the expression of other aquaporins.

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  • Physiological and molecular study of CO2 permeable aquaporins

    Grant number：26440146  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Katsuhara Maki, MORI Izumni

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    Grant amount：\5200000 （ Direct expense: \4000000 、 Indirect expense：\1200000 ）

    We established yeast screening system to identify CO2 permeable aquaporins. Among 69 aquaporins from rice, barley and Arabidopsis, 18 aquaporins were identified as putative CO2 permeable aquaporins. One of these aquaporins, OsTIP2;2 was detected around chloroplasts and some organelles (possible mitochondria), and seemed to have a function to supply CO2 intracellularly to inner chloroplast.

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  • Identification and analysis of rice and barley aquaporins as arsenite transporters.

    Grant number：20580063  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    KATSUHARA Maki

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    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    In this project, rice and barley aquaporins were selected and analyzed form the view to elucidate the molecular mechanism of arsenite transports in Poaceae plants. Final goal of this project is to breed rice/barley lines containing low As. Using screening system with yeast cells, 5 aquaporin genes (including known 3 genes) were identified as arsenite transporters. Among these genes, temporal increase of OsNIP2 ; 1 expression in roots and decrease of OsNIP3;3 in leaves were observed in rice plants treated with arsenite.

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  • Studies on Functional Analysis and Regulation Mechanism of Plant Aquaporins

    Grant number：19580106  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SHIBATA Hitoshi, KATSUHARA Maki

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    Grant amount：\3250000 （ Direct expense: \2500000 、 Indirect expense：\750000 ）

    チューリップ花弁の開閉は温度依存的であり、開花のためには水の花弁への輸送が必須で、水チャンネルの活性化が起こる。水チャネルを構成するアクアポリンの分子種を同定するために、細胞質アクアポリン遺伝子4種類をクローニングし、TgPIP2:2が水の輸送に関与することを機能面から確証した。またアクアポリンがリン酸化されて活性型になり、脱リン酸化で不活性化されることを確認した。TgPIP2:2も分子内でリン酸化を受ける3種のセリン残基(Ser35, Ser116, Ser274)を特定した。また、PIP2:2が花弁、茎、葉、塊茎、根いずれの組織においても最も発現量が多い分子種であり、根から花弁にいたる水の輸送に関与している可能性を指摘した。
    TgPIP2-2を酵母(ピキア)に発現させ、水チャンネル活性を図る手法を新たに導入した。リン酸化脱リン酸化の阻害剤が活性に影響したことから、簡便に水チャンネル活性を測定できる系であるとともに調節機構を検証するための優れた方法であると結論した。

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  • The elucidation of the low-temperature stress mechanism using reconstituted vesicles with tonoplast H^+-ATPase

    Grant number：11440237  1999 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KASAMO Kunihiro, KATSUHARA Maki, SHIBASAKA Mineo

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    Grant amount：\14200000 （ Direct expense: \14200000 ）

    Proton pumping across the tonoplast was markedly suppressed by chilling. In order to clarify the mechanism of chilling-induced depression of proton-pumping across tonoplast. we reconstituted chimera proteoliposomes with H^+-ATPase solubilized from the tonoplast of chilling insensitive rice (var. Nipponbare) without chilling and individual or combined lipids extracted from the tonoplast of chilling-sensitive rice (var. Boro) cells with or without chilling at 5 ℃. We further examined the relation between membrane fluidity and chilling.
    As the result, the chilling-induced decrease in proton pumping across the tonoplast is due to the decrease in the fluidity of the hydrophilic surface region of the lipid bilayer of the tonoplast, which is caused by glycolipids, such as glucocerebroside and acylsteryl glucoside.

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  • 塩ストレスによる細胞死誘導機構

    Grant number：10740370  1998 - 1999

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    且原 真木

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    Grant amount：\2200000 （ Direct expense: \2200000 ）

    プログラム細胞死(アポトーシス)は最近の研究から、植物細胞において道管形成などの形態形成や病原菌への抵抗性などで、中心的な役割をになう重要な細胞機構であると考えられるようになってきた。しかしその初期過程および分子メカニズムについてはまだ不明の点が多い。そこでこれらの点について、塩ストレスによって細胞死が誘導されるオオムギ根の実験系で研究をおこなった。
    動物細胞のアポトーシスにおいては、核DNAの断片化に先立って細胞膜インテグリティーの崩壊が起こるとされている。すなわち、通常では細胞膜脂質二重層の細胞質側に局在するフォスファチジルセリン(PS)の局在がくずれ、細胞外からPSに特異的に結合するアネキシンVによって細胞が標識されるようになる。昨年度の研究ではオオムギ根、オオムギ培養細胞、およびイネ培養細胞から単離したプロトプロストでは、塩ストレス処理によってはアネキシンVで標識されるようにはならない、という結果を報告した。今年度はプロトプロスト化しない、組織のままの根端を用いて、染色液を加圧して処理することにより染色することができることを見出した。すなわち塩ストレス処理後30分〜1時間で、一部の細胞がアネキシンVで標識されるようになることを明らかにした。
    また細胞死抑制遺伝子として、いくつかの植物種からdad1遺伝子が報告されたので、オオムギにおいても、そのホモログを検索した。PCR法を用いていくつかの産物を検出したが、塩基配列を解析した結果、既存のdad1と類似するものは見つからなかった。引き続きオオムギdad1の同定を進めているところである。

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  • 塩ストレスによる細胞死とストレスに適応した新根形成機構に関する細胞生理学的研究

    Grant number：09251213  1997

    日本学術振興会  科学研究費助成事業  重点領域研究

    且原 真木, 柴坂 三根夫

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    Grant amount：\1800000 （ Direct expense: \1800000 ）

    塩ストレスによってオオムギ根の伸長が抑制された時、塩ストレスの程度が中程度であったり、一過的であった場合は、新根が発生する。一方、この時いったんストレスを受けて伸長が停止した古い根については、根細胞では核DNA分解を伴う細胞死が起こっていることをこれまで明らかにした。この細胞核DNAの分解は、アポトーシス様の機構が働き、時間的にも早い反応であることから、何か積極的な細胞生理的意味があるのではないかと考えた。具体的には、ストレスを受けた根の物質を分解し、輸送して塩ストレスに適応した新しい根や、地上部の形成に再利用しているのではないかと仮説を立てた。この仮説を検証するために、実験をおこなった。また細胞死と根形成の関係についてさらなる知見を得るため、ヒトおよび線虫の細胞死抑制遺伝子を導入した形質転換タバコを用いて実験をおこなった。
    オオムギ根に^<32>Pまたは^3Hで標識したチミジンを根に取り込ませて、根細胞のDNAを標識した。一定の時間根に塩ストレスを与えた後、アポトーシス様の機構で分解された核酸成分が転流・再利用されて、新根あるいは地上部の核酸合成に利用されるまで待った後、新根、地上部から核酸を抽出して、取り込まれた放射能を測定した。その結果、ストレス解除後成長を再開した地上部に有意に標識が入ることが明らかになった。新根にも標識が入るようだが、地上部ほど明確な結果にならなかった。
    次にヒトおよび線虫の細胞死抑制遺伝子を導入した形質転換タバコを農水省農業生物資源研究所から提供を受けて、根の形態形成を観察した。形質転換タバコの根は成長が遅くなり、外見上はまっすぐに伸びず、縮れたようになっていた。維管束系を観察したところ、木部の構造に異常が生じた根が多く見られ、道管の通導が悪く、成熟が遅延していることがわかった。細胞死抑制因子が働いて、木部の組織分化・細胞配列に影響を与えた結果と推察された。これらの個体の塩ストレス応答について、現在研究を進めているところである。

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  • 高親和性カリウム輸送系の細胞生理学的研究

    Grant number：08740620  1996

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    且原 真木

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    Grant amount：\900000 （ Direct expense: \900000 ）

    本研究では資源作物オオムギからの高親和性カリウム輸送系遺伝子の同定し,環境および植物体の組織による高親和性カリウム輸送系発現の調節を解明することを目指した。
    酵母から数種、植物ではコムギから1種類報告されていた高親和性カウム輸送系遺伝子において認められた共通のアミノ酸配列部分に対応する20から30の塩基配列を人工合成した。上流プライマーとして5種類(TRK1、TRK3、TRK5、MH1、MH2)作成した。下流プライマーとして4種類(TRK2、TRK4、HR2、HR3)作成した。
    高親和性カリウム輸送系は、カリウム欠乏条件で発現していると考えられている。そこで標準の培養液からカリウム塩を除いた条件で、2週間水耕栽培したオオムギ(品種赤神力)の根からRNAを抽出し、cDNAに変換した後、上記プライマーを上流、下流いろいろな組み合わせを使って高親和性カリウム輸送系遺伝子のPCRによる増幅を試みた。様々なPCR条件を検討したが結局いかなる組み合わせを用いてもPCR産物を得ることができなかった。名古屋大学のグループによると(未発表データ)、アラビドプシスにおいて、TRK1(5'-GGIAAYACIYTITTYCC-3')とTRK2(5'-VDIGGIARRTACATCAT-3')の組み合わせで、高親和性カリウム輸送系遺伝子を増幅することができたという。オオムギでは、このプライマーの組み合わせでもPCR産物は得ることができなかったので、アラビドプシスとは、遺伝子の塩基配列が多少異なるのかも知れない。あるいは、今回用いた栽培条件では高親和性カリウム輸送系が発現していなかったという可能性もあるので、現在栽培条件を変えて(0.25mMの硫酸カルシウムのみの培地)1週間育てたオオムギからRNAを抽出して、再度PCRをおこなっている。

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  • カリウム輸送系の細胞生理学および分子生物学的研究

    Grant number：07740622  1995

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    且原 真木

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    Grant amount：\900000 （ Direct expense: \900000 ）

    カリウムは植物の3大栄養の一つであり、また高濃度NaClが存在する塩ストレス条件下ではナトリウムイオンと拮抗するカリウムイオン吸収能力が耐塩性機構に深く関与すると考えられている。そこでカリウム輸送系の細胞生理学および分子生物学的研究を、Mesembryanthemun crystallinumおよびオオムギを研究材料としておこなった。前者については、カリウムチャンネル遺伝子断片がPCR法によって同定されていたので、その成果に基づき今年度は遺伝子の全長と遺伝子発現について調べた。その結果この遺伝子のcDNAは2,800bpの長さであることがわかった。またこの遺伝子は主として根で発現していた。PCR法によると、葉では根のものと異なった長さのDNAが増幅されてきたので、根とは異なる種類のカリウムチャンネルが発現しているものと考えられる。塩ストレスによる影響については、この遺伝子の発現は塩ストレスによりやや減少することが明らかになった。発現部位と塩ストレスの影響についてさらに詳細に検討するため、in situ PCR法の導入を現在検討している。つぎにオオムギのカリウム輸送系遺伝子については、Mesembryanthemun crystallinumと同じ条件では、目的とする遺伝子はPCRによって増幅できなかった。そこでPCRの条件を検討した結果、いくつかのPCR産物を得ることができた。これらが目的とするものかどうか、現在クローニングと塩基配列決定の準備を進めている。

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  • Studies on Salinity Tolerance of mineral translocation in halophyte roots.

    Grant number：06660080  1994 - 1995

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for General Scientific Research (C)

    SHIBASAKA Mineo, KATSUHARA Maki, NAKASHIMA Susumu

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    Grant amount：\1900000 （ Direct expense: \1900000 ）

    This research project was aimed on determining the characteristics of plasmamembrane contributed to salinity tolerance of a halophyte, Salicornia virginica, roots, based on a physiological and biochemical comparison with mesophyte. Though integrity of mesophyte plasmamembranes has been known to be loss under conditions of high concentration of salt, it has not been clear that what changes in the membranes causes the damage. We planed to investigate what changes in the membranes enhanced permeability and how much important metabolites flowed through the membranes.
    Treatments of high NaCl conc.solutions diminished absorption of potassium of a mesophyte, Hordeum vulgare. By the contrast, these treatments enhanced that of a halophyte, Salicornia. We found that important metabolites flowed out of barley roots treated by high NaCl conc.solutions and the high energy bonds was lost. Decrement of potassium uptake in barley could be caused by the loss of energy, which was determined by ^<31>P-NMR and a biochemical quantitation. The loss of membrane integrity was detected by taking up a fluorescent substance (propidium iodide). Releasing peripheral proteins from the membranes and decreasing of plasmamembrane ATPase apoprotein were found simultaneously. However, relationship between these phenomenon and increment of membrane permeability has not be clarified.
    Because gene expression needs energy, introducing salinity tolerant gene to barley will be a failure without improving plasmamembrane.

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