研究者詳細 - 渡部　昌実

論文 - 渡部　昌実

分割表示 266 件中 181 - 200 件目／全件表示 >>

A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector 査読

Masami Watanabe, Masakiyo Sakaguchi, Rie Kinoshita, Haruki Kaku, Yuichi Ariyoshi, Hideo Ueki, Ryuta Tanimoto, Shin Ebara, Kazuhiko Ochiai, Junichiro Futami, Shun-Ai Li, Peng Huang, Yasutomo Nasu, Nam-Ho Huh, Hiromi Kumon

ONCOLOGY REPORTS 31 ( 3 ) 1089 - 1095 2014年3月

Adenovirus-mediated REIC/Dkk-3 gene therapy: Development of an autologous cancer vaccination therapy (Review) 査読

Masami Watanabe, Yasutomo Nasu, Hiromi Kumon

ONCOLOGY LETTERS 7 ( 3 ) 595 - 601 2014年3月

Anti-Cancer Effects of REIC/Dkk-3-encoding Adenoviral Vector for the Treatment of Non-small Cell Lung Cancer 査読

Kazuhiko Shien, Norimitsu Tanaka, Masami Watanabe, Junichi Soh, Masakiyo Sakaguchi, Keitaro Matsuo, Hiromasa Yamamoto, Masashi Furukawa, Hiroaki Asano, Kazunori Tsukuda, Yasutomo Nasu, Nam-Ho Huh, Shinichiro Miyoshi, Hiromi Kumon, Shinichi Toyooka

PLOS ONE 9 ( 2 ) 2014年2月

N’-[4-(dipropylamino)benzylidene]-2-hydroxybenzohydrazide is a dynamin GTPase inhibitor that suppresses cancer cell migration and invasion by inhibiting actin polymerization . 査読

Hiroshi Yamada, Tadashi Abe, Shun-Ai Li, Shota Tago, Peng Huang, Masami Watanabe, Satoru Ikeda, Naohisa Ogo, Akira Asai, Kohji Takei

Biochem Biophys Res Commun 443 ( 2 ) 511 - 517 2014年1月

A rare complication: Misdirection of an indwelling urethral catheter into the ureter 査読

Tsutomu Ishikaw, Motoo Araki, Takeshi Hirata, Masami Watanabe, Shin Ebara, Toyohiko Watanabe, Yasutomo Nasu, Hiromi Kumon

Acta Medica Okayama 68 ( 1 ) 47 - 51 2014年

Comparison of Incubation Solutions Prior to the Purification of Porcine Islet Cells. 国際誌

Takashi Kawai, Hirofumi Noguchi, Takashi Kuise, Atsuko Nakatsuka, Akihiro Katayama, Noriko Imagawa, Hitomi Usui Kataoka, Issei Saitoh, Yasufumi Noguchi, Masami Watanabe, Toshiyoshi Fujiwara

Cell medicine 6 ( 1-2 ) 9 - 14 2013年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

For pancreatic islet transplantation, one of the most important steps of islet isolation is islet purification. The most common method of islet purification is density gradient centrifugation because there are differences in density between islets and acinar tissue. However, the density of islets/acinar tissue depends on several conditions, such as the incubation time before purification and the osmolality of the preincubation solution. In this study, we evaluated the impact of using two different preincubation solutions before purification. We used the University of Wisconsin (UW) solution and a new preservation solution (HN-1), which we recently developed. There were no significant differences between the two solutions in terms of the islet yield, rate of viability, and purity or stimulation index after purification. There were also no differences in the attainability and suitability of posttransplantation normoglycemia. Our study shows that the HN-1 solution is equivalent to the UW solution for preincubation before islet purification.

DOI： 10.3727/215517913X674180

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Comparison of New Preservation Solutions, HN-1 and University of Wisconsin Solution, in Pancreas Preservation for Porcine Islet Isolation. 査読国際誌

Katayama Akihiro, Noguchi Hirofumi, Kuise Takashi, Nakatsuka Atsuko, Hirota Daisho, Kataoka Hitomi, Kawai Takashi, Inoue Kentaro, Imagawa Noriko, Saitoh Issei, Noguchi Yasufumi, Watanabe Masami, Wada Jun, Fujiwara Toshiyoshi

Cell Medicine 6 ( 1-2 ) 3 - 8 2013年12月

Testosterone replacement elevates the serum uric acid levels in patients with female to male gender identity disorder 査読

Hiroaki Kurahashi, Masami Watanabe, Morito Sugimoto, Yuichi Ariyoshi, Sabina Mahmood, Motoo Araki, Kazushi Ishii, Yasutomo Nasu, Atsushi Nagai, Hiromi Kumon

ENDOCRINE JOURNAL 60 ( 12 ) 1321 - 1327 2013年12月

Culture Conditions for Mouse Pancreatic Stem Cells. 国際誌

Hirofumi Noguchi, Issei Saitoh, Hitomi Usui Kataoka, Masami Watanabe, Yasufumi Noguchi, Toshiyoshi Fujiwara

Cell medicine 5 ( 2-3 ) 63 - 8 2013年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Recently, mouse pancreatic stem cells have been isolated from adult mouse pancreata. However, these pancreatic stem cells could be maintained only under specific culture conditions with lot-limited fetal bovine serum (FBS). For the efficient isolation and maintenance of mouse pancreatic stem cells, it is important to identify culture conditions that can be used independent of the FBS lot. In this study, we evaluated the culture conditions required to maintain mouse pancreatic stem cells. The mouse pancreatic stem cells derived from the pancreas of a newborn mouse, HN#101, were cultured under the following conditions: 1) Dulbecco's modified Eagle's medium (DMEM) with 20% lot-limited FBS, in which mouse pancreatic stem cells could be cultured without changes in morphology and growth activity; 2) complete embryonic stem (ES) cell media; and 3) complete ES cell media on feeder layers of mitomycin C-treated STO cells, which were the same culture conditions used for mouse ES cells. Under culture conditions #1 and #3, the HN#101 cells continued to form a flat "cobblestone" monolayer and continued to divide actively beyond the population doubling level (PDL) 100 without growth inhibition, but this did not occur under culture condition #2. The gene expression profile and differentiated capacity of the HN#101 cells cultured for 2 months under culture condition #3 were similar to those of HN#101 cells at PDL 50. These data suggest that complete ES cell media on feeder layers could be useful for maintaining the undifferentiated state of pancreatic stem cells.

DOI： 10.3727/215517913X666495

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Isolation Efficiency of Mouse Pancreatic Stem Cells Is Age Dependent. 国際誌

Takashi Kuise, Hirofumi Noguchi, Issei Saitoh, Hitomi Usui Kataoka, Masami Watanabe, Yasufumi Noguchi, Toshiyoshi Fujiwara

Cell medicine 5 ( 2-3 ) 69 - 73 2013年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Mouse pancreatic stem cells have been isolated from mouse pancreata. This study evaluated the efficacy of isolating mouse pancreatic stem cells using mice of different ages. The pancreata of newborn mice, 8-week-old mice, and 24-week-old mice were harvested and digested by using collagenase. The "duct-like" cells in the digested pancreatic tissue were then inoculated into 96-well plates, cloned by limiting dilution, and cultured in DMEM with 20% FBS. Pancreatic stem cells were isolated from the pancreata of all newborn mice, while cells could only be isolated from 10% of the pancreata of 8-week-old mice and could not be isolated from the pancreata of any 24-week-old mice. These data suggest that young mice may have some pancreatic stem cells and that older mice may only have a few pancreatic stem cells. These data also indicate that it is extremely difficult to isolate pancreatic stem cells from older mice, suggesting that future research focus its efforts on finding methods of isolating pancreatic stem cells from adult mice.

DOI： 10.3727/215517913X666503

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Comparison of incubation solutions prior to the purification of porcine islet cells. 査読

Kawai Takashi, Noguchi Hirofumi, Kuise Takashi, Nakatsuka Atsuko, Katayama Akihiro, Imagawa Noriko, Kataoka, Hitomi Usui, Saitoh Issei, Noguchi Yasufumi, Watanabe Masami, Fujiwara Toshiyoshi

Cell Medicine 2013年10月

Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours 査読

Kazuhiko Ochiai, Masami Watanabe, Daigo Azakami, Masaki Michishita, Yasunaga Yoshikawa, Chihiro Udagawa, Pornphimon Metheenukul, Thippayarat Chahomchuen, Hiroshi Aoki, Hiromi Kumon, Masami Morimatsu, Toshinori Omi

VETERINARY JOURNAL 197 ( 3 ) 769 - 775 2013年9月

Clinical significance of CD24 as a predictor of bladder cancer recurrence 査読

Chunxiao Liu, Shaobo Zheng, Haiyan Shen, Kai Xu, Jie Chen, Hulin Li, Yawen Xu, Abai Xu, Binshen Chen, Haruki Kaku, Yasutomo Nasu, Hiromi Kumon, Peng Huang, Masami Watanabe

ONCOLOGY LETTERS 6 ( 1 ) 96 - 100 2013年7月

Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia. 査読国際誌

Hiroshi Yamada, Tadashi Abe, Ayano Satoh, Nana Okazaki, Shota Tago, Kinue Kobayashi, Yumi Yoshida, Yoshiya Oda, Masami Watanabe, Kazuhito Tomizawa, Hideki Matsui, Kohji Takei

The Journal of neuroscience : the official journal of the Society for Neuroscience 33 ( 10 ) 4514 - 26 2013年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Dynamin GTPase, a key molecule in endocytosis, mechanically severs the invaginated membrane upon GTP hydrolysis. Dynamin functions also in regulating actin cytoskeleton, but the mechanisms are yet to be defined. Here we show that dynamin 1, a neuronal isoform of dynamin, and cortactin form ring complexes, which twine around F-actin bundles and stabilize them. By negative-staining EM, dynamin 1-cortactin complexes appeared as "open" or "closed" rings depending on guanine nucleotide conditions. By pyrene actin assembly assay, dynamin 1 stimulated actin assembly in mouse brain cytosol. In vitro incubation of F-actin with both dynamin 1 and cortactin led to the formation of long and thick actin bundles, on which dynamin 1 and cortactin were periodically colocalized in puncta. A depolymerization assay revealed that dynamin 1 and cortactin increased the stability of actin bundles, most prominently in the presence of GTP. In rat cortical neurons and human neuroblastoma cell line, SH-SY5Y, both dynamin 1 and cortactin localized on actin filaments and the bundles at growth cone filopodia as revealed by immunoelectron microscopy. In SH-SY5Y cell, acute inhibition of dynamin 1 by application of dynamin inhibitor led to growth cone collapse. Cortactin knockdown also reduced growth cone filopodia. Together, our results strongly suggest that dynamin 1 and cortactin ring complex mechanically stabilizes F-actin bundles in growth cone filopodia. Thus, the GTPase-dependent mechanochemical enzyme property of dynamin is commonly used both in endocytosis and regulation of F-actin bundles by a dynamin 1-cortactin complex.

DOI： 10.1523/JNEUROSCI.2762-12.2013

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Dose-response analysis of testosterone replacement therapy in patients with female to male gender identity disorder 査読

Aya Nakamura, Masami Watanabe, Morito Sugimoto, Tomoko Sako, Sabina Mahmood, Haruki Kaku, Yasutomo Nasu, Kazushi Ishii, Atsushi Nagai, Hiromi Kumon

ENDOCRINE JOURNAL 60 ( 3 ) 275 - 281 2013年3月

In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle 査読

Koichi Oishi, Yoshitaka Miyamoto, Hiroaki Saito, Katsutoshi Murase, Kenji Ono, Makoto Sawada, Masami Watanabe, Yasufumi Noguchi, Toshiyoshi Fujiwara, Shuji Hayashi, Hirofumi Noguchi

PLoS ONE 8 ( 2 ) 2013年2月

Culture conditions for mouse pancreatic stem cells. 査読

Noguchi H, Saitoh I, Kataoka HU, Watanabe M, Fujiwara T

Cell Medicine. 5 63 - 68 2013年1月

Isolation efficiency of mouse pancreatic stem cells is age-dependent. 査読

Kuise T, Noguchi H, Saitoh I, Kataoka HU, Watanabe M, Fujiwara T

Cell Medicine. 5 69 - 73 2013年1月

Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line. 査読国際誌

Peng Huang, Masami Watanabe, Haruki Kaku, Hideo Ueki, Hirofumi Noguchi, Morito Sugimoto, Takeshi Hirata, Hiroshi Yamada, Kohji Takei, Shaobo Zheng, Kai Xu, Yasutomo Nasu, Yasuyuki Fujii, Chunxiao Liu, Hiromi Kumon

Molecular and clinical oncology 1 ( 1 ) 180 - 184 2013年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Cancer stem cells (CSCs) are thought to be crucial for understanding the biological roots of cancer, and are of increasing importance as a target for new anticancer agents. According to an expression analysis of the cell surface antigens of various types of cancer, CD133 is considered to be a potential marker of cancer stemness. In this study, a human urinary bladder cancer cell line (J82) was used to analyze the cancer stem cell-like characteristics of CD133+ bladder cancer cells in vitro and in vivo. The CD133 expression in the J82 cells was examined and the cells were immunomagnetically categorized into positive and negative subsets. The CD133- and CD133+ subsets were phenotypically divergent with regard to the cell growth pattern, while CD133+ cells tended to colonize during their growth. In CD133+ cells, the pluripotent stem cell factors Oct-4 and Sox-2 were upregulated, and a statistically significant proliferation increase was observed when compared to CD133- cells. The CD133+ subpopulation was more tolerant to the chemotherapeutic agent cisplatin, and Bacillus Calmette-Guérin (BCG), an agent instilled intravesically to treat bladder cancer. In addition, CD133+ J82 cells were more resistant to radiation treatment when compared to CD133- cells. The in vivo tumorigenesis of the CD133- and CD133+ subsets of J82 cancer cells was also examined by subcutaneously injecting them into nude mice. The tumor growth was more aggressive in the CD133+ subpopulation, showing a significant difference in the tumorigenic potential in these subsets. In conclusion, J82 human bladder cancer cells include CD133- and CD133+ subpopulations, while the CD133 molecule is a potential marker of the potential malignancy of human bladder cancer. In the present study, the CD133+ subpopulation was herein demonstrated to have certain characteristics consistent with those of cancer stem cells.

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DIRECT THERAPEUTIC EFFECT OF REIC/DKK-3-ENCODING ADENOVIRAL VECTOR FOR NON-SMALL CELL LUNG CANCER 査読

Yamamoto Hiromasa, Tanaka Norimitsu, Toyooka Shinichi, Watanabe Masami, Sakaguchi Masakiyo, Soh Junichi, Shien Kazuhiko, Furukawa Masashi, Asano Hiroaki, Tsukuda Kazunori, Nasu Yasutomo, Huh Nam-ho, Kumon Hiromi, Miyoshi Shinichirou

JOURNAL OF THORACIC ONCOLOGY 7 ( 11 ) S463 2012年11月

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