Faculty Profiles - NAMBA Hikaru

MISC - NAMBA Hikaru

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High-resolution immuno-scanning electron microscopy using a non-coating method: study of herpes simplex virus glycoproteins on the surface of virus particles and infected cells. Reviewed International journal

J A Padilla, F Uno, M Yamada, H Namba, S Nii

Journal of electron microscopy 46 ( 2 ) 171 - 80 1997

Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：OXFORD UNIV PRESS

The expression of two glycoproteins, i.e. glycoprotein C (gC) and glycoprotein D (gD), of herpes simplex virus type 1 (HSV-1) on the surface of extracellular particles of this virus was examined by immuno-scanning electron microscopy. Scanning electron microscopy specimens of infected cells immuno-labelled against the glycoproteins with colloidal gold particles were prepared by a conventional coating and a non-coating method. Surface ultrastructure of infected cells and gold particles were observed more clearly with specimens prepared by the non-coating method. The appearance of virus particles in association with glycoprotein expression on these particles and on the surface of infected cells was then studied. Progeny virus particles began to appear 6 h after infection, increased in number as the infection proceeded, and covered most of the cell surface by 16 h. Six to 24 h after the infection, the labelling density for each glycoprotein on virus particles remained constant. The labelling density for gD was always higher than that for gC. The patch-like distribution of gold-labelling against gD was often detected on infected cell monolayers at the exponential and late stage of one cycle of virus growth. The labelling density for gD on virus particles was the highest on these produced in Vero and L-929 cells, moderate in MRC-5, BHK-21 and FL cells, and the lowest in HEp-2 cells.

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BDNF gene can Be activated by Ca2+ signals without involvement of de novo AP-1 synthesis. International journal

K Sano, H Nanba, A Tabuchi, T Tsuchiya, M Tsuda

Biochemical and biophysical research communications 229 ( 3 ) 788 - 93 1996.12

Language：English Publisher：ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS

Although stimulation of N-methyl-D-aspartate receptors or voltage-dependent calcium channels induces both the activation of c-fos and brain-derived neurotrophic factor (BDNF) genes, it is not certain how the activation of these genes is related. Using primary cultures of rat hippocampal neurons, we found that exposing the cells to cycloheximide allowed subsequent activation of BDNF mRNA expression, although activation of AP-1 DNA-binding activity resulting from the c-fos induction was abolished. Super-induction of BDNF gene was also caused by cycloheximide. The estimated half-life of BDNF mRNA was approximately 2.5 hrs, which was almost identical to that of c-fos mRNA. These results indicate that nascent AP-1 is not required for the activation of BDNF gene, leading to the notion that the BDNF gene can be activated by Ca2+ signals as an immediate early gene.

DOI： 10.1006/bbrc.1996.1881

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インフルエンザウイルスの細胞侵入に対するセシウム添加の影響(in vitro)

山下信子, 小川寛人, 難波ひかる, 山田雅夫

臨床とウイルス 48 ( 3 ) S91 - S91 2020.9

唾液からのHHV6とHHV7のDNA検出率の前方視的検討保育園入園半年と非就園半年の比較

鳥越貞義, 渡辺正博, 難波ひかる, 山下信子, 小川寛人, 山田雅夫, 菅秀, 根来麻奈美

臨床とウイルス 47 ( 2 ) S84 - S84 2019.4

セシウムがインフルエンザウイルス増殖に及ぼす影響

山下信子, 小川寛人, 難波ひかる, 山田雅夫

日本ウイルス学会学術集会プログラム・予稿集(Web) 67th 2019

岡山大学自然生命科学研究支援センターに搬入される家畜ブタのE型肝炎ウイルス感染状況について

小川寛人, 平山晴子, 田中爽暉, 矢田範夫, 難波ひかる, 山下信子, 米満研三, 前田健, 樅木勝巳, 山田雅夫

岡山実験動物研究会報 ( 35 ) 2019

ヒトヘルペスウイルス6Bのテグメント蛋白質間の相互作用解析

難波ひかる

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

HHV6Bテグメント蛋白質間相互作用の網羅的解析

難波ひかる, 小川寛人, 山下信子, 山田雅夫

日本ウイルス学会学術集会プログラム・予稿集(Web) 67th 2019

養豚農場から大学動物実験施設に搬入される家畜ブタのE型肝炎ウイルス感染状況について

小川寛人, 平山晴子, 田中爽暉, 矢田範夫, 難波ひかる, 山下信子, 米満研三, 前田健, 樅木勝巳, 山田雅夫

日本獣医学会学術集会講演要旨集 161回 475 - 475 2018.8

動物実験施設に搬入される家畜ブタにおけるE型肝炎ウイルスの遺伝子検出および血清学的解析

小川寛人, 難波ひかる, 山下信子, 山田雅夫, 田中爽暉, 矢田範夫, 平山晴子, 樅木勝巳, 米満研三, 前田健

臨床とウイルス 46 ( 2 ) S58 - S58 2018.4

抗GFP-Nanobody共免疫沈降法によるウイルステグメント蛋白質の相互作用解析

難波ひかる

生命科学系学会合同年次大会 2017年度 [3LBA - 114] 2017.12

Metabolic pathway catalyzed by Vanin-1 pantetheinase plays a suppressive role in influenza virus replication in human alveolar epithelial A549 cells. International journal

Nobuko Yamashita, Masato Yashiro, Hirohito Ogawa, Hikaru Namba, Nobuyuki Nosaka, Yousuke Fujii, Tsuneo Morishima, Hirokazu Tsukahara, Masao Yamada

Biochemical and biophysical research communications 489 ( 4 ) 466 - 471 2017.8

Language：English Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE

Our previous analysis of gene expression profiles in the peripheral blood from patients with influenza A (H1N1) pdm09 pneumonia revealed elevated transcription levels of the vanin-1 (vascular non-inflammatory molecule 1, VNN1) gene, which encodes an epithelial ectoenzyme with pantetheinase activity involved in recycling coenzyme A. Here, to elucidate the role of VNN1 in influenza A virus (IAV) H1N1 infection, we investigated the change of VNN1 expression in the context of IAV infection and the effects of its related substances, i.e., its direct substrate pantetheine and its two metabolites pantothenic acid and cysteamine on the replication of IAV in the human alveolar epithelial carcinoma cell line A549. The messenger RNA expression of VNN1 in A549 cells was significantly increased (by 4.9-fold) after IAV infection under an elevated concentration of pantetheine. Moreover, VNN1 mRNA levels were elevated by > 100-fold in response to pro-inflammatory cytokines, especially TNF-α and IL-1β. Pantetheine significantly reduced the IAV replication and IAV Matrix 1 (M1) mRNA levels when it was administered prior to and during infection. In addition, cysteamine treatment during IAV infection significantly reduced the viral replication and IAV M1 mRNA levels, whereas pantothenic acid did not. These findings suggest that the metabolic pathway catalyzed by VNN1 pantetheinase plays a suppressive role in IAV infection in the respiratory tract, especially in severe conditions under hypercytokinemia.

DOI： 10.1016/j.bbrc.2017.05.172

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抗GFP-Nanobody共免疫沈降法によるウイルステグメント蛋白質の相互作用解析

難波ひかる

日本生化学会大会(Web) 90th [3LBA - 114] 2017

集団保育児における唾液中のHHV-6とHHV-7DNAの排泄

難波ひかる, 渡辺正博, 鳥越貞義, 山下信子, 山田雅夫

臨床とウイルス 45 ( 2 ) S76 - S76 2017

突発性発疹の感染様式に関する研究

鳥越貞義, 難波ひかる, 渡辺正博, 宮崎裕樹, 森島恒雄, 山田雅夫

臨床とウイルス 43 ( 2 ) S111 - S111 2015

Vanin-1がインフルエンザウイルス感染に果たす役割

山下信子, 難波ひかる, 山田雅夫, 森島恒雄

臨床とウイルス 41 ( 2 ) S97 - S97 2013.5

インフルエンザウイルス増殖に対する酸化ストレス関連遺伝子Vanin-1の影響

山下信子, 難波ひかる, 山田雅夫, 森島恒雄

日本ウイルス学会学術集会プログラム・抄録集 61st 2013

HHV-6Bテグメント蛋白質の単独発現系による細胞内局在解析

難波ひかる, 山下信子, 山田雅夫

日本ウイルス学会学術集会プログラム・抄録集 60th 2012

Vanin-1がインフルエンザウイルス感染に果たす役割

山下信子, 山下信子, 難波ひかる, 山田雅夫, 森島恒雄

日本ウイルス学会学術集会プログラム・抄録集 60th 2012

Enhancive effects of D-glucose and its analogs on expression of d-glucose-unrelated transgenes in mammalian cells. Reviewed

Miyuki Kimura, Hikaru Namba, Manabu Okubo, Mai Ezumi, Nao Susumu, Masao Yamada, Yujiro Arao

Journal of bioscience and bioengineering 112 ( 2 ) 194 - 201 2011.8

Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN

We studied the effects of d-glucose on transgene expression in mammalian cells by a reporter gene assay using CV-1 cells and a CMV promoter-controlled EGFP gene. Treatment of CV-1 cells with 5% D-glucose unchanged the number of fluorescent cells in fluorescence microscopic observation but significantly intensified fluorescence in the fluorometric assay. Furthermore, EGFP itself and mRNA became more abundant in Western blot and quantitative RT-PCR analyses of 5% D-glucose-treated cells, respectively. These results indicate that elevated D-glucose can activate transgene expression via transcriptional stimulation, at least in part. The same concentrations of L-glucose led to only negligible increases in transgene expression, indicating that D-glucose's effect is different from its osmotic effect. The D-glucose-induced augmentation of fluorescence was observed not only in the experiment using the CMV promoter-controlled EGFP gene but also in experiments using the SV40 and RSV promoter-controlled ones, suggesting that elevated D-glucose can enhance transgene expression regulated by various promoters commonly used in transgene expression. The assessment of D-glucose analogs for their enhancive effects on transgene expression revealed that 1,6-anhydro-D-glucose and β-methyl-D-glucoside had stronger effects than D-glucose. From this result, we can expect to find more effective carbohydrates to enhance transgene expression. The α- and β-M-D-glucosides, which are slightly different from each other in three-dimensional structure, exerted largely distinct stimulative effects on transgene expression, suggesting that fundamental rules determine the enhancive effects of saccharides and that the modification of the saccharide by applying such rules will enable us to develop more powerful substances for transgene expression.

DOI： 10.1016/j.jbiosc.2011.04.010

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