Faculty Profiles - HIRAYAMA Takashi

写真a

HIRAYAMA Takashi

Organization

Scheduled update Professor

External link

Degree

博士（理学） ( 京都大学 )

Research Interests

plat stress response system
植物環境ストレス応答
情報伝達機構
植物ホルモン
シロイヌナズナ

Research Areas

Life Science / Plant molecular biology and physiology

Education

京都大学理学研究科

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名古屋大学理学部

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Research History

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2010

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Yokohama City University Graduate School of Nanobioscience, Graduate

2009 - 2010

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横浜市立大学大学院総合理学研究科生体超分子システム科学専攻客員准教授

2001 - 2009

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ペンシルバニア大学博士研究員

1996 - 1998

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理化学研究所研究員

1992 - 2010

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Kyoto University Institute for Chemical Research

1989 - 1992

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Professional Memberships

日本植物生理学会

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日本分子生物学会

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Papers

Identification and characterization of functional DOG1 residues regulating the abscisic acid response in Arabidopsis

Noriyuki Nishimura, Wataru Tsuchiya, Nahomi Suzuki, Takashi Hirayama, Toshimasa Yamazaki

The Plant Journal 2025.4

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Publishing type：Research paper (scientific journal)

DOI： 10.1111/tpj.70180

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Responses of the marine filamentous red alga ‘Bangia’ sp. ESS1 to recurrent changes in seawater concentration Reviewed

Puja Kumari, Ryo Matsushima, Takashi Hirayama, Koji Mikami

Algal Research 80 103551 - 103551 2024.6

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Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.algal.2024.103551

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Synchronization between chloroplastic and cytosolic protein synthesis for photosynthesis complex assembly

Tomoya Fujita, Taisei Wakigawa, Naohiro Kawamoto, Yukio Kurihara, Yuu Hirose, Takashi Hirayama, Hirotaka Toh, Tomoko Kuriyama, Atsushi Hashimoto, Eriko Matsuura-Suzuki, Keiichi Mochida, Minoru Yoshida, Minami Matsui, Shintaro Iwasaki

2024.5

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DOI： 10.1101/2024.05.03.592458

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Application of Trehalose Mitigates Short-Styled Flowers in Solanaceous Crops Reviewed

Izumi C. Mori, Takakazu Matsuura, Masahiro Otao, Lia Ooi, Yasuyo Nishimura, Takashi Hirayama

Journal of Agricultural and Food Chemistry 71 ( 14 ) 5476 - 5482 2023.4

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Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.jafc.2c08479

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Exome-wide variation in a diverse barley panel reveals genetic associations with ten agronomic traits in Eastern landraces Reviewed

June-Sik Kim, Kotaro Takahagi, Komaki Inoue, Minami Shimizu, Yukiko Uehara-Yamaguchi, Asaka Kanatani, Daisuke Saisho, Ryuei Nishii, Alexander E. Lipka, Takashi Hirayama, Kazuhiro Sato, Keiichi Mochida

Journal of Genetics and Genomics 2022.12

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Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.jgg.2022.12.001

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FE UPTAKE‐INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis Reviewed International journal

Satoshi Okada, Gui J. Lei, Naoki Yamaji, Sheng Huang, Jian F. Ma, Keiichi Mochida, Takashi Hirayama

Plant, Cell & Environment 45 ( 11 ) 3322 - 3337 2022.11

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Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

FE UPTAKE-INDUCING PEPTIDE1 (FEP1), also named IRON MAN3 (IMA3) is a short peptide involved in the iron deficiency response in Arabidopsis thaliana. Recent studies uncovered its molecular function, but its physiological function in the systemic Fe response is not fully understood. To explore the physiological function of FEP1 in iron homoeostasis, we performed a transcriptome analysis using the FEP1 loss-of-function mutant fep1-1 and a transgenic line with oestrogen-inducible expression of FEP1. We determined that FEP1 specifically regulates several iron deficiency-responsive genes, indicating that FEP1 participates in iron translocation rather than iron uptake in roots. The iron concentration in xylem sap under iron-deficient conditions was lower in the fep1-1 mutant and higher in FEP1-induced transgenic plants compared with the wild type (WT). Perls staining revealed a greater accumulation of iron in the cortex of fep1-1 roots than in the WT root cortex, although total iron levels in roots were comparable in the two genotypes. Moreover, the fep1-1 mutation partially suppressed the iron overaccumulation phenotype in the leaves of the oligopeptide transporter3-2 (opt3-2) mutant. These data suggest that FEP1 plays a pivotal role in iron movement and in maintaining the iron quota in vascular tissues in Arabidopsis.

DOI： 10.1111/pce.14424

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.14424
Plant Hormonomics: A Key Tool for Deep Physiological Phenotyping to Improve Crop Productivity. Invited Reviewed

Takashi Hirayama, Keiichi Mochida

Plant & cell physiology 2022.5

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

Agriculture is particularly vulnerable to climate change. To cope with the risks posed by climate-related stressors to agricultural production, global population growth, and changes in food preferences, it is imperative to develop new climate-smart crop varieties with increased yield and environmental resilience. Molecular genetics and genomic analyses have revealed that allelic variations in genes involved in phytohormone-mediated growth regulation have greatly improved productivity in major crops. Plant science has remarkably advanced our understanding of the molecular basis of various phytohormone-mediated events in plant life. These findings provide essential information for improving the productivity of crops growing in changing climates. In this review, we highlight the recent advances in plant hormonomics (multiple phytohormone profiling) and discuss their application to crop improvement. We present plant hormonomics as a key tool for deep physiological phenotyping, focusing on representative plant growth regulators associated with the improvement of crop productivity. Specifically, we review advanced methodologies in plant hormonomics, highlighting mass spectrometry- and nanosensor-based plant hormone profiling techniques. We also discuss the applications of plant hormonomics in crop improvement through breeding and agricultural management practices.

DOI： 10.1093/pcp/pcac067

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PARN-like Proteins Regulate Gene Expression in Land Plant Mitochondria by Modulating mRNA Polyadenylation Invited Reviewed

Takashi Hirayama

International Journal of Molecular Sciences 22 ( 19 ) 10776 - 10776 2021.10

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：{MDPI} {AG}

DOI： 10.3390/ijms221910776

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Genetic Elucidation for Response of Flowering Time to Ambient Temperatures in Asian Rice Cultivars Reviewed

Kiyosumi Hori, Daisuke Saisho, Kazufumi Nagata, Yasunori Nonoue, Yukiko Uehara-Yamaguchi, Asaka Kanatani, Koka Shu, Takashi Hirayama, Jun-ichi Yonemaru, Shuichi Fukuoka, Keiichi Mochida

International Journal of Molecular Sciences 22 ( 3 ) 1024 - 1024 2021.1

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Climate resilience of crops is critical for global food security. Understanding the genetic basis of plant responses to ambient environmental changes is key to developing resilient crops. To detect genetic factors that set flowering time according to seasonal temperature conditions, we evaluated differences of flowering time over years by using chromosome segment substitution lines (CSSLs) derived from japonica rice cultivars “Koshihikari” × “Khao Nam Jen”, each with different robustness of flowering time to environmental fluctuations. The difference of flowering times in 9 years’ field tests was large in “Khao Nam Jen” (36.7 days) but small in “Koshihikari” (9.9 days). Part of this difference was explained by two QTLs. A CSSL with a “Khao Nam Jen” segment on chromosome 11 showed 28.0 days’ difference; this QTL would encode a novel flowering-time gene. Another CSSL with a segment from “Khao Nam Jen” in the region around Hd16 on chromosome 3 showed 23.4 days” difference. A near-isogenic line (NIL) for Hd16 showed 21.6 days’ difference, suggesting Hd16 as a candidate for this QTL. RNA-seq analysis showed differential expression of several flowering-time genes between early and late flowering seasons. Low-temperature treatment at panicle initiation stage significantly delayed flowering in the CSSL and NIL compared with “Koshihikari”. Our results unravel the molecular control of flowering time under ambient temperature fluctuations.

DOI： 10.3390/ijms22031024

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Temperature-dependent fasciation mutants provide a link between mitochondrial RNA processing and lateral root morphogenesis. Reviewed

Kurataka Otsuka, Akihito Mamiya, Mineko Konishi, Mamoru Nozaki, Atsuko Kinoshita, Hiroaki Tamaki, Masaki Arita, Masato Saito, Kayoko Yamamoto, Takushi Hachiya, Ko Noguchi, Takashi Ueda, Yusuke Yagi, Takehito Kobayashi, Takahiro Nakamura, Yasushi Sato, Takashi Hirayama, Munetaka Sugiyama

eLife 10 2021.1

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Publishing type：Research paper (scientific journal) Publisher：eLife Sciences Publications, Ltd

Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, <italic>root redifferentiation defective 1</italic> (<italic>rrd1</italic>), <italic>rrd2</italic>, and <italic>root initiation defective 4</italic> (<italic>rid4</italic>), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e. fasciated) LRs under high-temperature conditions due to extra cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.

DOI： 10.7554/elife.61611

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The barley pan-genome reveals the hidden legacy of mutation breeding Reviewed

Jayakodi, M., Padmarasu, S., Haberer, G., Bonthala, V.S., Gundlach, H., Monat, C., Lux, T., Kamal, N., Lang, D., Himmelbach, A., Ens, J., Zhang, X.-Q., Angessa, T.T., Zhou, G., Tan, C., Hill, C., Wang, P., Schreiber, M., Boston, L.B., Plott, C., Jenkins, J., Guo, Y., Fiebig, A., Budak, H., Xu, D., Zhang, J., Wang, C., Grimwood, J., Schmutz, J., Guo, G., Zhang, G., Mochida, K., Hirayama, T., Sato, K., Chalmers, K.J., Langridge, P., Waugh, R., Pozniak, C.J., Scholz, U., Mayer, K.F.X., Spannagl, M., Li, C., Mascher, M., Stein, N.

Nature 588 ( 7837 ) 284 - 289 2020.12

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Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

DOI： 10.1038/s41586-020-2947-8

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BdWRKY38 is required for the incompatible interaction of Brachypodium distachyon with the necrotrophic fungus Rhizoctonia solani Reviewed International journal

Yusuke Kouzai, Minami Shimizu, Komaki Inoue, Yukiko Uehara‐Yamaguchi, Kotaro Takahagi, Risa Nakayama, Takakazu Matsuura, Izumi C. Mori, Takashi Hirayama, Sobhy S. H. Abdelsalam, Yoshiteru Noutoshi, Keiichi Mochida

The Plant Journal 104 ( 4 ) 995 - 1008 2020.11

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Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Rhizoctonia solani is a soil-borne necrotrophic fungus that causes sheath blight in grasses. The basal resistance of compatible interactions between R. solani and rice is known to be modulated by some WRKY transcription factors (TFs). However, genes and defense responses involved in incompatible interaction with R. solani remain unexplored, because no such interactions are known in any host plants. Recently, we demonstrated that Bd3-1, an accession of the model grass Brachypodium distachyon, is resistant to R. solani and, upon inoculation with the fungus, undergoes rapid induction of genes responsive to the phytohormone salicylic acid (SA) that encode the WRKY TFs BdWRKY38 and BdWRKY44. Here, we show that endogenous SA and these WRKY TFs positively regulate this accession-specific R. solani resistance. In contrast to a susceptible accession (Bd21), the infection process in the resistant accessions Bd3-1 and Tek-3 was suppressed at early stages before the development of fungal biomass and infection machinery. A comparative transcriptome analysis during pathogen infection revealed that putative WRKY-dependent defense genes were induced faster in the resistant accessions than in Bd21. A gene regulatory network (GRN) analysis based on the transcriptome dataset demonstrated that BdWRKY38 was a GRN hub connected to many target genes specifically in resistant accessions, whereas BdWRKY44 was shared in the GRNs of all three accessions. Moreover, overexpression of BdWRKY38 increased R. solani resistance in Bd21. Our findings demonstrate that these resistant accessions can activate an incompatible host response to R. solani, and BdWRKY38 regulates this response by mediating SA signaling.

DOI： 10.1111/tpj.14976

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.14976
Exploration of Life-Course Factors Influencing Phenotypic Outcomes in Crops Reviewed

Keiichi Mochida, Alexander E Lipka, Takashi Hirayama

Plant and Cell Physiology 61 ( 8 ) 1381 - 1383 2020.8

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Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

DOI： 10.1093/pcp/pcaa087

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Other Link： http://academic.oup.com/pcp/article-pdf/61/8/1381/33662834/pcaa087.pdf
Decoding Plant–Environment Interactions That Influence Crop Agronomic Traits Reviewed

Keiichi Mochida, Ryuei Nishii, Takashi Hirayama

Plant and Cell Physiology 61 ( 8 ) 1408 - 1418 2020.8

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Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

<title>Abstract</title>To ensure food security in the face of increasing global demand due to population growth and progressive urbanization, it will be crucial to integrate emerging technologies in multiple disciplines to accelerate overall throughput of gene discovery and crop breeding. Plant agronomic traits often appear during the plants’ later growth stages due to the cumulative effects of their lifetime interactions with the environment. Therefore, decoding plant–environment interactions by elucidating plants’ temporal physiological responses to environmental changes throughout their lifespans will facilitate the identification of genetic and environmental factors, timing and pathways that influence complex end-point agronomic traits, such as yield. Here, we discuss the expected role of the life-course approach to monitoring plant and crop health status in improving crop productivity by enhancing the understanding of plant–environment interactions. We review recent advances in analytical technologies for monitoring health status in plants based on multi-omics analyses and strategies for integrating heterogeneous datasets from multiple omics areas to identify informative factors associated with traits of interest. In addition, we showcase emerging phenomics techniques that enable the noninvasive and continuous monitoring of plant growth by various means, including three-dimensional phenotyping, plant root phenotyping, implantable/injectable sensors and affordable phenotyping devices. Finally, we present an integrated review of analytical technologies and applications for monitoring plant growth, developed across disciplines, such as plant science, data science and sensors and Internet-of-things technologies, to improve plant productivity.

DOI： 10.1093/pcp/pcaa064

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Other Link： http://academic.oup.com/pcp/article-pdf/61/8/1408/33662755/pcaa064.pdf
Life-course monitoring of endogenous phytohormone levels under field conditions reveals diversity of physiological states among barley accessions. Reviewed

Takashi Hirayama, Daisuke Saisho, Takakazu Matsuura, Satoshi Okada, Kotaro Takahagi, Asaka Kanatani, Jun Ito, Hiroyuki Tsuji, Yoko Ikeda, Keiichi Mochida

Plant & cell physiology 61 ( 8 ) 1438 - 1448 2020.4

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Agronomically important traits often develop during the later stages of crop growth as consequences of various plant-environment interactions. Therefore, the temporal physiological states that change and accumulate during the crop's life course can significantly affect the eventual phenotypic differences in agronomic traits among crop varieties. Thus, to improve productivity, it is important to elucidate the associations between temporal physiological responses during the growth of different crop varieties and their agronomic traits. However, data representing the dynamics and diversity of physiological states in plants grown under field conditions is sparse. In this study, we quantified the endogenous levels of five phytohormones-auxin, cytokinins, abscisic acid, jasmonate, and salicylic acid-in the leaves of eight diverse barley (Hordeum vulgare) accessions grown under field conditions sampled weekly over their life course to assess the ongoing fluctuations in hormone levels in the different accessions under field growth conditions. Notably, we observed enormous changes over time in the development-related plant hormones, such as auxin and cytokinins. Using 3' RNA-seq-based transcriptome data from the same samples, we investigated the expression of barley genes orthologous to known hormone-related genes of Arabidopsis throughout the life course. These data illustrated the dynamics and diversity of the physiological states of these field-grown barley accessions. Together our findings provide new insights into plant-environment interaction, highlighting that there is cultivar diversity in physiological responses during growth under field conditions.

DOI： 10.1093/pcp/pcaa046

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Hormonal and transcriptional analyses of fruit development and ripening in different varieties of black pepper (Piper nigrum)

Choy-Yuen Khew, Izumi C. Mori, Takakazu Matsuura, Takashi Hirayama, Jennifer Ann Harikrishna, Ee-Tiing Lau, Zehnder Jarroop Augustine Mercer, Siaw-San Hwang

Journal of Plant Research 133 ( 1 ) 73 - 94 2020.1

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Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media {LLC}

Black pepper (Piper nigrum L.) is one of the most popular and oldest spices in the world with culinary uses and various pharmacological properties. In order to satisfy the growing worldwide demand for black pepper, improved productivity of pepper is highly desirable. A primary constraint in black pepper production is the non-synchronous nature of flower development and non-uniform fruit ripening within a spike. The uneven ripening of pepper berries results in a high labour requirement for selective harvesting contributes to low productivity and affects the quality of the pepper products. In Malaysia, there are a few recommended varieties for black pepper planting, each having some limitations in addition to the useful characteristics. Therefore, a comparative study of different black pepper varieties will provide a better understanding of the mechanisms regulates fruit development and ripening. Plant hormones are known to influence the fruit development process and their roles in black pepper flower and fruit development were inferred based on the probe-based gene expression analysis and the quantification of the multiple plant hormones using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). In this study, jasmonic acid and salicylic acid were found to play roles in flowering and fruit setting, whereas auxin, gibberellin and cytokinins are important for fruit growth. Abscisic acid has positive role in fruit maturation and ripening in the development process. Distinct pattern of plant hormones related gene expression profiles with the hormones accumulation profiles suggested a complex network of regulation is involved in the signaling process and crosstalk between plant hormones was another layer of regulation in the black pepper fruit development mechanisms. The current study provides clues to help in elucidating the timing of the action of each specific plant hormone during fruit development and ripening which could be applied to enhance our ability to control the ripening process, leading to improving procedures for the production and post-harvest handling of pepper fruits.

DOI： 10.1007/s10265-019-01156-0

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Plant hormone profiling in developing seeds of common wheat (Triticum aestivum L.). Reviewed

Takakazu Matsuura, Izumi C Mori, Eiko Himi, Takashi Hirayama

Breeding science 69 ( 4 ) 601 - 610 2019.12

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This study examined contents of nine plant hormones in developing seeds of field-grown wheat varieties (Triticum aestivum L.) with different seed dormancy using liquid chromatography-mass spectrometry. The varieties showed marked diversity in germination indices at 15°C and 20°C. Contents of the respective hormones in seeds showed a characteristic pattern during seed maturation from 30-day post anthesis to 60-day post anthesis. Principal component analysis and hierarchical clustering analysis revealed that plant hormone profiles were not correlated with dormancy levels, indicating that hormone contents were not associated with preharvest sprouting (PHS) susceptibility. Indole acetic acid (IAA) contents of mature seeds showed positive correlation with the germination index, but no other hormone. Response of embryo-half seeds to exogenous abscisic acid (ABA) indicates that ABA sensitivity is correlated with whole-seed germinability, which can be explained in part by genotypes of MOTHER OF FT AND TFL (MFT) allele modulating ABA signaling of wheat seeds. These results demonstrate that variation in wheat seed dormancy is attributable to ABA sensitivity of mature seeds, but not to ABA contents in developing seeds.

DOI： 10.1270/jsbbs.19030

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Regulation of the poly(A) status of mitochondrial mRNA by poly(A) specific ribonuclease is conserved among land plants. Reviewed

Kanazawa M, Ikeda Y, Nishihama R, Yamaoka S, Lee NH, Yamato KT, Kohchi T, Hirayama T

Plant & Cell Physiology 61 ( 3 ) 470 - 480 2019.11

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

<title>Abstract</title>
Regulation of the stability and the quality of mitochondrial RNA is essential for the maintenance of mitochondrial and cellular functions in eukaryotes. We have previously reported that the eukaryotic poly(A)-specific ribonuclease (PARN) and the prokaryotic poly(A) polymerase encoded by AHG2 and AGS1, respectively, coordinately regulate the poly(A) status and the stability of mitochondrial mRNA in Arabidopsis. Mitochondrial function of PARN has not been reported in any other eukaryotes. To know how much this PARN-based mitochondrial mRNA regulation is conserved among plants, we studied the AHG2 and AGS1 counterparts of the liverwort, Marchantia polymorpha, a member of basal land plant lineage. We found that M. polymorpha has one ortholog each for AHG2 and AGS1, named MpAHG2 and MpAGS1, respectively. Their Citrine-fused proteins were detected in mitochondria of the liverwort. Molecular genetic analysis showed that MpAHG2 is essential and functionally interacts with MpAGS1 as observed in Arabidopsis. A recombinant MpAHG2 protein had a deadenylase activity in vitro. Overexpression of MpAGS1 and the reduced expression of MpAHG2 caused an accumulation of polyadenylated Mpcox1 mRNA. Furthermore, MpAHG2 suppressed Arabidopsis ahg2-1 mutant phenotype. These results suggest that the PARN-based mitochondrial mRNA regulatory system is conserved in land plants.

DOI： 10.1093/pcp/pcz212

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Other Link： http://academic.oup.com/pcp/article-pdf/61/3/470/32891632/pcz212.pdf
Transcriptome analysis and identification of a transcriptional regulatory network in the response to H<inf>2</inf>O<inf>2</inf> Reviewed

Ayaka Hieno, Hushna Ara Naznin, Keiko Inaba-Hasegawa, Tomoko Yokogawa, Natsuki Hayami, Mika Nomoto, Yasuomi Tada, Takashi Yokogawa, Mieko Higuchi-Takeuchi, Kosuke Hanada, Minami Matsui, Yoko Ikeda, Yuko Hojo, Takashi Hirayama, Kazutaka Kusunoki, Hiroyuki Koyama, Nobutaka Mitsuda, Yoshiharu Y. Yamamoto

Plant Physiology 180 ( 3 ) 1629 - 1646 2019.7

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DOI： 10.1104/pp.18.01426

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Overexpression of Prunus DAM6 inhibits growth, represses bud break competency of dormant buds and delays bud outgrowth in apple plants Reviewed

Yamane, Hisayo, Wada, Masato, Honda, Chikako, Matsuura, Takakazu, Ikeda, Yoko, Hirayama, Takashi, Osako, Yutaro, Gao-Takai, Mei, Kojima, Mikiko, Sakakibara, Hitoshi, Tao, Ryutaro

PLOS ONE 14 ( 4 ) e0214788 2019.4

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1371/journal.pone.0214788

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Computer vision-based phenotyping for improvement of plant productivity: a machine learning perspective Reviewed

Keiichi Mochida, Satoru Koda, Komaki Inoue, Takashi Hirayama, Shojiro Tanaka, Ryuei Nishii, Farid Melgani

GigaScience 8 ( 1 ) 2019.1

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Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

DOI： 10.1093/gigascience/giy153

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Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme Reviewed

Noriyuki Nishimura, Wataru Tsuchiya, James J. Moresco, Yuki Hayashi, Kouji Satoh, Nahomi Kaiwa, Tomoko Irisa, Toshinori Kinoshita, Julian I. Schroeder, John R. Yates, Takashi Hirayama, Toshimasa Yamazaki

Nature Communications 9 ( 1 ) 2132 2018.12

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Language：English Publishing type：Research paper (scientific journal) Publisher：Nature Publishing Group

DOI： 10.1038/s41467-018-04437-9

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Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages. Reviewed

Takagi H, Watanabe S, Tanaka S, Matsuura T, Mori IC, Hirayama T, Shimada H, Sakamoto A

BMC plant biology 18 ( 1 ) 287 2018.11

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1186/s12870-018-1491-2

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Loss of CG methylation in Marchantia polymorpha causes disorganization of cell division and reveals unique DNA methylation regulatory mechanisms of non-CG methylation. Reviewed

Ikeda Y, Nishihama R, Yamaoka S, Arteaga-Vazquez MA, Aguilar-Cruz A, Grimanelli D, Pogorelcnik R, Martienssen RA, Yamato KT, Kohchi T, Hirayama T, Mathieu O

Plant & cell physiology 2018.8

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Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcy161

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The putative peptide gene FEP1 regulates iron deficiency response in Arabidopsis. Reviewed

Hirayama T, Lei GJ, Yamaji N, Nakagawa N, Ma JF

Plant & cell physiology 59 ( 9 ) 1739 - 1752 2018.7

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcy145

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The Mechanism of SO₂ -Induced Stomatal Closure Differs from O₃ and CO₂ Responses and Is Mediated by Non-Apoptotic Cell Death in Guard Cells. Reviewed International journal

Ooi L, Matsuura T, Munemasa S, Murata Y, Katsuhara M, Hirayama T, Mori IC

Plant, cell & environment 42 ( 2 ) 437 - 447 2018.7

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Language：English Publishing type：Research paper (scientific journal)

Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism.

DOI： 10.1111/pce.13406

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Salicylic acid-dependent immunity contributes to resistance against Rhizoctonia solani, a necrotrophic fungal agent of sheath blight, in rice and Brachypodium distachyon Reviewed

Yusuke Kouzai, Mamiko Kimura, Megumi Watanabe, Kazuki Kusunoki, Daiki Osaka, Tomoko Suzuki, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Takakazu Matsuura, Izumi C. Mori, Takashi Hirayama, Eiichi Minami, Yoko Nishizawa, Komaki Inoue, Yoshihiko Onda, Keiichi Mochida, Yoshiteru Noutoshi

New Phytologist 217 ( 2 ) 771 - 783 2018.1

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Language：English Publishing type：Research paper (scientific journal) Publisher：Blackwell Publishing Ltd

DOI： 10.1111/nph.14849

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Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner (vol 67, pg 2519, 2016) Reviewed

Hiroshi Takagi, Yasuhiro Ishiga, Shunsuke Watanabe, Tomokazu Konishi, Mayumi Egusa, Nobuhiro Akiyoshi, Takakazu Matsuura, Izumi C. Mori, Takashi Hirayama, Hironori Kaminaka, Hiroshi Shimada, Atsushi Sakamoto

JOURNAL OF EXPERIMENTAL BOTANY 68 ( 17 ) 5011 - 5011 2017.8

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Phytohormones in red seaweeds: a technical review of methods for analysis and a consideration of genomic data Reviewed

Izumi C. Mori, Yoko Ikeda, Takakazu Matsuura, Takashi Hirayama, Koji Mikami

BOTANICA MARINA 60 ( 2 ) 153 - 170 2017.4

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DOI： 10.1515/bot-2016-0056

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Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds Reviewed

Koji Mikami, Izumi C. Mori, Takakazu Matsuura, Yoko Ikeda, Mikiko Kojima, Hitoshi Sakakibara, Takashi Hirayama

JOURNAL OF APPLIED PHYCOLOGY 28 ( 4 ) 2539 - 2548 2016.8

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DOI： 10.1007/s10811-015-0759-2

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ahg12 is a dominant proteasome mutant that affects multiple regulatory systems for germination of Arabidopsis Reviewed

Shimpei Hayashi, Takashi Hirayama

SCIENTIFIC REPORTS 6 2016.5

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DOI： 10.1038/srep25351

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Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner Reviewed

Hiroshi Takagi, Yasuhiro Ishiga, Shunsuke Watanabe, Tomokazu Konishi, Mayumi Egusa, Nobuhiro Akiyoshi, Takakazu Matsuura, Izumi C. Mori, Takashi Hirayama, Hironori Kaminaka, Hiroshi Shimada, Atsushi Sakamoto

JOURNAL OF EXPERIMENTAL BOTANY 67 ( 8 ) 2519 - 2532 2016.4

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DOI： 10.1093/jxb/erw071

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Crop improvement using life cycle datasets acquired under field conditions Reviewed

Keiichi Mochida, Daisuke Saisho, Takashi Hirayama

FRONTIERS IN PLANT SCIENCE 6 ( September ) 2015.9

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DOI： 10.3389/fpls.2015.00740

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ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis (vol 66, pg 2763, 2015) Reviewed

Yu Lu, Yuki Sasaki, Xingwen Li, Izumi C. Mori, Takakazu Matsuura, Takashi Hirayama, Takeo Sato, Junji Yamaguchi

JOURNAL OF EXPERIMENTAL BOTANY 66 ( 15 ) 4851 - 4851 2015.8

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Abscisic acid induces ectopic outgrowth in epidermal cells through cortical microtubule reorganization in Arabidopsis thaliana Reviewed

Shogo Takatani, Takashi Hirayama, Takashi Hashimoto, Taku Takahashi, Hiroyasu Motose

SCIENTIFIC REPORTS 5 2015.6

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DOI： 10.1038/srep11364

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ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis Reviewed

Lu, Y., Sasaki, Y., Li, X., Mori, I.C., Matsuura, T., Hirayama, T., Sato, T., Yamaguchij, J.

Journal of Experimental Botany 66 ( 9 ) 2763 - 2771 2015

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DOI： 10.1093/jxb/erv086

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A unique system for regulating mitochondrial mRNA poly(A) status and stability in plants Reviewed

Takashi Hirayama

Plant Signaling and Behavior 9 ( 10 ) 1 - 4 2014

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DOI： 10.4161/15592324.2014.973809

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Elucidation of the RNA Recognition Code for Pentatricopeptide Repeat Proteins Involved in Organelle RNA Editing in Plants Reviewed

Yusuke Yagi, Shimpei Hayashi, Keiko Kobayashi, Takashi Hirayama, Takahiro Nakamura

PLOS ONE 8 ( 3 ) 2013.3

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DOI： 10.1371/journal.pone.0057286

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A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis. Reviewed International journal

Hirayama T, Matsuura T, Ushiyama S, Narusaka M, Kurihara Y, Yasuda M, Ohtani M, Seki M, Demura T, Nakashita H, Narusaka Y, Hayashi S

Nature communications 4 2247 - 2247 2013

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Coordination of gene expression in the organelles and the nucleus is important for eukaryotic cell function. Transcriptional and post-transcriptional gene regulation in mitochondria remains incompletely understood in most eukaryotes, including plants. Here we show that poly(A)-specific ribonuclease, which influences the poly(A) status of cytoplasmic mRNA in many eukaryotes, directly regulates the poly(A) tract of mitochondrial mRNA in conjunction with a bacterial-type poly(A) polymerase, AGS1, in Arabidopsis. An Arabidopsis poly(A)-specific ribonuclease-deficient mutant, ahg2-1, accumulates polyadenylated mitochondrial mRNA and shows defects in mitochondrial protein complex levels. Mutations of AGS1 suppress the ahg2-1 phenotype. Mitochondrial localizations of AHG2 and AGS1 are required for their functions in the regulation of the poly(A) tract of mitochondrial mRNA. Our findings suggest that AHG2 and AGS1 constitute a regulatory system that controls mitochondrial mRNA poly(A) status in Arabidopsis.

DOI： 10.1038/ncomms3247

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Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing Reviewed

Maki Murayama, Shimpei Hayashi, Noriyuki Nishimura, Mayumi Ishide, Keiko Kobayashi, Yusuke Yagi, Tadao Asami, Takahiro Nakamura, Kazuo Shinozaki, Takashi Hirayama

JOURNAL OF EXPERIMENTAL BOTANY 63 ( 14 ) 5301 - 5310 2012.9

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DOI： 10.1093/jxb/ers188

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Multiple hormone treatment revealed novel cooperative relationships between abscisic acid and biotic stress hormones in cultured cells Reviewed

Mami Okamoto, Yuuri Tsuboi, Hideki Goda, Takeshi Yoshizumi, Yukihisa Shimada, Takashi Hirayama

PLANT BIOTECHNOLOGY 29 ( 1 ) 19 - 34 2012

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DOI： 10.5511/plantbiotechnology.11.1130a

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An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis Reviewed

June-Sik Kim, Junya Mizoi, Takuya Yoshida, Yasunari Fujita, Jun Nakajima, Teppei Ohori, Daisuke Todaka, Kazuo Nakashima, Takashi Hirayama, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki

PLANT AND CELL PHYSIOLOGY 52 ( 12 ) 2136 - 2146 2011.12

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DOI： 10.1093/pcp/pcr143

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The Regulatory Networks of Plant Responses to Abscisic Acid Reviewed

Taishi Umezawa, Takashi Hirayama, Takashi Kuromori, Kazuo Shinozaki

PLANT RESPONSES TO DROUGHT AND SALINITY STRESS: DEVELOPMENTS IN A POST-GENOMIC ERA 57 201 - 248 2011

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DOI： 10.1016/B978-0-12-387692-8.00006-0

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A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis Reviewed

Yoshinori Shingu, Tsutomu Mikawa, Mariko Onuma, Takashi Hirayama, Takehiko Shibata

FEBS JOURNAL 277 ( 10 ) 2360 - 2374 2010.5

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DOI： 10.1111/j.1742-4658.2010.07651.x

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Ds Transposon Mutant Lines for Saturation Mutagenesis of the Arabidopsis genome Reviewed

Takashi Kuromori, Takashi Hirayama

The Handbook of Plant Mutation Screening: Mining of Natural and Induced Alleles 17 - 30 2010.3

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DOI： 10.1002/9783527629398.ch2

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Research on plant abiotic stress responses in the post-genome era: past, present and future Reviewed

Takashi Hirayama, Kazuo Shinozaki

PLANT JOURNAL 61 ( 6 ) 1041 - 1052 2010.3

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DOI： 10.1111/j.1365-313X.2010.04124.x

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The PP2c-SnRK2 complex: The central regulator of an abscisic acid signaling pathway Reviewed

Hirayama, T., Umezawa, T.

Plant Signaling and Behavior 5 ( 2 ) 160 - 163 2010.2

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DOI： 10.4161/psb.5.2.10460

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ABA Hypersensitive Germination2-1 Causes the Activation of Both Abscisic Acid and Salicylic Acid Responses in Arabidopsis Reviewed

Noriyuki Nishimura, Mami Okamoto, Mari Narusaka, Michiko Yasuda, Hideo Nakashita, Kazuo Shinozaki, Yoshihiro Narusaka, Takashi Hirayama

PLANT AND CELL PHYSIOLOGY 50 ( 12 ) 2112 - 2122 2009.12

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DOI： 10.1093/pcp/pcp146

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Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Reviewed

Umezawa, T, Sugiyama, Y, Mizoguchi, M, Hayashi, S, Myouga, F, Yamaguchi-Shinozaki, K, Ishihama, Y, Hirayama, T, Shinozaki, K

Proc. Nat. Acad. Sci. USA. 106 ( 41 ) 17588 - 17593 2009.9

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DOI： 10.1073/pnas.0907095106

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Metabolic movement upon abscisic acid and salicylic acid combined treatments Reviewed

Okamoto Mami, Tsuboi Yuuri, Chikayama Eisuke, Kikuchi Jun, Hirayama Takashi

Plant Biotechnology 26 ( 5 ) 551 - 560 2009

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Plant hormones are known to play important roles for maintenance of internal conditions under various environmental stresses. Recent studies revealed that there is a significant cross-talk between abiotic and biotic stress responses. To understand such complex mechanisms, comprehensive analyses at multiple levels are required. In this study, to examine the dynamic interactions between plant hormone responses, we analyzed the metabolic movements of Arabidopsis thaliana cultured cells during hormone treatments by NMR metabolic profiling. First, we verified the effect of plant hormone treatments on intracellular metabolites, and detected that the abscisic acid (ABA), salicylic acid (SA), auxin, and brassinosteroid treatment caused metabolic changes. Secondly, since SA and ABA act antagonistically against each other, we monitored dynamic metabolic movements during ABA and SA combined treatments. The response to ABA-only treatment suggested that sugars and amino acids significantly increased. Although SA alone caused fewer metabolic changes, SA caused remarkable metabolic changes when applied in combination with ABA. In addition, our NMR data implied that salicylate glucoside (SAG), which is major metabolite converted from SA, significantly increased in the SA-only treatment but decreased with ABA in a dose dependent manner. These results suggest that ABA and SA cross-talk at the metabolite level in a complicate manner and that the combination of various conditions will provide us with a holistic view of plant stress response mechanisms.

DOI： 10.5511/plantbiotechnology.26.551

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Systematic NMR Analysis of Stable Isotope Labeled Metabolite Mixtures in Plant and Animal Systems: Coarse Grained Views of Metabolic Pathways Reviewed

Eisuke Chikayama, Michitaka Suto, Takashi Nishihara, Kazuo Shinozaki, Takashi Hirayama, Jun Kikuchi

PLOS ONE 3 ( 11 ) e3805 2008.11

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DOI： 10.1371/journal.pone.0003805

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The Glycerophosphoryl Diester Phosphodiesterase-Like Proteins SHV3 and its Homologs Play Important Roles in Cell Wall Organization Reviewed

Shimpei Hayashi, Tadashi Ishii, Toshiro Matsunaga, Rumi Tominaga, Takashi Kuromori, Takuji Wada, Kazuo Shinozaki, Takashi Hirayama

PLANT AND CELL PHYSIOLOGY 49 ( 10 ) 1522 - 1535 2008.10

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DOI： 10.1093/pcp/pcn120

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Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene Reviewed

Hiroki Ukitsu, Takashi Kuromori, Kiminori Toyooka, Yumi Goto, Ken Matsuoka, Eiji Sakuradani, Sakayu Shimizu, Asako Kamiya, Yuko Imura, Masahiro Yuguchi, Takuji Wada, Takashi Hirayama, Kazuo Shinozaki

PLANT AND CELL PHYSIOLOGY 48 ( 11 ) 1524 - 1533 2007.11

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DOI： 10.1093/pcp/pcm139

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Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA Reviewed

Takashi Hirayama, Kazuo Shinozaki

TRENDS IN PLANT SCIENCE 12 ( 8 ) 343 - 351 2007.8

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DOI： 10.1016/j.tplants.2007.06.013

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ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed Reviewed

Noriyuki Nishimura, Tomo Yoshida, Nobutaka Kitahata, Tadao Asami, Kazuo Shinozaki, Takashi Hirayama

PLANT JOURNAL 50 ( 6 ) 935 - 949 2007.6

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DOI： 10.1111/j.1365-313X.2007.03107.x

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Top-down phenomics of Arabidopsis thaliana: metabolic profiling by one- and two-dimensional nuclear magnetic resonance spectroscopy and transcriptome analysis of albino mutants. Reviewed

Tian C, Chikayama E, Tsuboi Y, Kuromori T, Shinozaki K, Kikuchi J, Hirayama T

The Journal of biological chemistry 282 ( 25 ) 18532 - 18541 2007.6

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DOI： 10.1074/jbc.M700549200

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Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance Reviewed

Satoshi Iuchi, Hiroyuki Koyama, Atsuko Iuchi, Yasufumi Kobayashi, Sadako Kitabayashi, Yuriko Kobayashi, Takashi Ikka, Takashi Hirayama, Kazuo Shinozaki, Masatomo Kobayashi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104 ( 23 ) 9900 - 9905 2007.6

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DOI： 10.1073/pnas.0700117104

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Loss of Necrotic Spotted Lesions 1 associates with cell death and defense responses in Arabidopsis thaliana Reviewed

Yoshiteru Noutoshi, Takashi Kuromori, Takuji Wada, Takashi Hirayama, Asako Kamiya, Yuko Imura, Michiko Yasuda, Hideo Nakashita, Ken Shirasu, Kazuo Shinozaki

PLANT MOLECULAR BIOLOGY 62 ( 1-2 ) 29 - 42 2006.9

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DOI： 10.1007/s11103-006-9001-6

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A trial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis Reviewed

Takashi Kuromori, Takuji Wada, Asako Kamiya, Masahiro Yuguchi, Takuro Yokouchi, Yuko Imura, Hiroko Takabe, Tetsuya Sakurai, Kenji Akiyama, Takashi Hirayama, Kiyotaka Okada, Kazuo Shinozaki

PLANT JOURNAL 47 ( 4 ) 640 - 651 2006.8

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DOI： 10.1111/j.1365-313X.2006.02808.x

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ABA-Hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs Reviewed

T Yoshida, N Nishimura, N Kitahata, T Kuromori, T Ito, T Asami, K Shinozaki, T Hirayama

PLANT PHYSIOLOGY 140 ( 1 ) 115 - 126 2006.1

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DOI： 10.1104/pp.105.070128

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Analysis of ABA Hypersensitive Germination2 revealed the pivotal functions of PARN in stress response in Arabidopsis Reviewed

N Nishimura, N Kitahata, M Seki, Y Narusaka, M Narusaka, T Kuromori, T Asami, K Shinozaki, T Hirayama

PLANT JOURNAL 44 ( 6 ) 972 - 984 2005.12

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DOI： 10.1111/j.1365-313X.2005.02589.x

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Isolation and characterization of novel mutants affecting the abscisic acid sensitivity of Arabidopsis germination and seedling growth Reviewed

N Nishimura, T Yoshida, M Murayama, T Asami, K Shinozaki, T Hirayama

PLANT AND CELL PHYSIOLOGY 45 ( 10 ) 1485 - 1499 2004.10

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DOI： 10.1093/pcp/pch171

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Quantitative trait loci analysis of nitrate storage in Arabidopsis leading to an investigation of the contribution of the anion channel gene, AtCLC-c, to variation in nitrate levels

H Harada, T Kuromori, T Hirayama, K Shinozaki, RA Leigh

JOURNAL OF EXPERIMENTAL BOTANY 55 ( 405 ) 2005 - 2014 2004.9

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DOI： 10.1093/jxb/erh224

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AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis Reviewed

K Takei, N Ueda, K Aoki, T Kuromori, T Hirayama, K Shinozaki, T Yamaya, H Sakakibara

PLANT AND CELL PHYSIOLOGY 45 ( 8 ) 1053 - 1062 2004.8

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DOI： 10.1093/pcp/pch119

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Stable isotope labeling of Arabidopsis thaliana for an NMR-based metabolomics approach Reviewed

J Kikuchi, K Shinozaki, T Hirayama

PLANT AND CELL PHYSIOLOGY 45 ( 8 ) 1099 - 1104 2004.8

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RCH1, a locus in Arabidopsis That confers resistance to the hemibiotrophic fungal pathogen Colletotrichum higginsianum Reviewed

Y Narusaka, M Narusaka, P Park, Y Kubo, T Hirayama, M Seki, T Shiraishi, J Ishida, M Nakashima, A Enju, T Sakurai, M Satou, M Kobayashi, K Shinozaki

MOLECULAR PLANT-MICROBE INTERACTIONS 17 ( 7 ) 749 - 762 2004.7

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A novel Arabidopsis gene required for ethanol tolerance is conserved among plants and archaea Reviewed

N Fujishige, N Nishimura, S Iuchi, T Kunii, K Shinozaki, T Hirayama

PLANT AND CELL PHYSIOLOGY 45 ( 6 ) 659 - 666 2004.6

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DOI： 10.1093/pcp/pch086

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A novel ethanol-hypersensitive mutant of Arabidopsis Reviewed

T Hirayama, N Fujishige, T Kunii, N Nishimura, S Iuchi, K Shinozaki

PLANT AND CELL PHYSIOLOGY 45 ( 6 ) 703 - 711 2004.6

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DOI： 10.1093/pcp/pch078

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A collection of 11,800 single-copy Ds transposon insertion lines in Arabidopsis Reviewed

T Kuromori, T Hirayama, Y Kiyosue, H Takabe, S Mizukado, T Sakurai, K Akiyama, A Kamiya, T Ito, K Shinozaki

PLANT JOURNAL 37 ( 6 ) 897 - 905 2004.3

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DOI： 10.1111/j.1365-313X.2003.02009.x

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Stable isotope labeling of Arabidopsis thaliana for an NMR-based metabolomics approach Reviewed

Kikuchi, J., Shinozaki, K., Hirayama, T.

Plant and Cell Physiology 45 ( 8 ) 1099 - 1104 2004

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DOI： 10.1093/pcp/pch117

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Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes Reviewed

Takahashi, N., Kuroda, H., Kuromori, T., Hirayama, T., Seki, M., Shinozaki, K., Shimada, H., Matsui, M.

Plant and Cell Physiology 45 ( 1 ) 83 - 91 2004

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DOI： 10.1093/pcp/pch009

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Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in arabidopsis cell culture Reviewed

S Takahashi, T Katagiri, T Hirayama, K Yamaguchi-Shinozaki, K Shinozaki

PLANT AND CELL PHYSIOLOGY 42 ( 2 ) 214 - 222 2001.2

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DOI： 10.1093/pcp/pce028

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Ethylene captures a metal! Metal ions are involved in ethylene perception and signal transduction Reviewed

T Hirayama, JM Alonso

PLANT AND CELL PHYSIOLOGY 41 ( 5 ) 548 - 555 2000.5

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DOI： 10.1093/pcp/41.5.548

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A transmembrane hybrid-type histidine kinase in arabidopsis functions as an osmosensor Reviewed

T Urao, B Yakubov, R Satoh, K Yamaguchi-Shinozaki, M Seki, T Hirayama, K Shinozaki

PLANT CELL 11 ( 9 ) 1743 - 1754 1999.9

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DOI： 10.1105/tpc.11.9.1743

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EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis Reviewed

JM Alonso, T Hirayama, G Roman, S Nourizadeh, Ecker, JR

SCIENCE 284 ( 5423 ) 2148 - 2152 1999.6

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DOI： 10.1126/science.284.5423.2148

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Responsive-to-antagonist1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis Reviewed

T Hirayama, JJ Kieber, N Hirayama, M Kogan, P Guzman, S Nourizadeh, JM Alonso, WP Dailey, A Dancis, Ecker, JR

CELL 97 ( 3 ) 383 - 393 1999.4

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DOI： 10.1016/S0092-8674(00)80747-3

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Molecular responses to water stress in Arabidopsis thaliana. Reviewed

Shinozaki, K, Yamaguchi-Shinozaki, K, Mizoguchi, T, Urao, T, Katagiri, T, Nakashima, K, Abe, H, Ichimura, K, Liu, Q, Nanjo, T, Uno, Y, Iuchi, S, Seki, M, Ito, T, Hirayama, T, Mikami, K

J. Plant Res. 111 ( 1102 ) 345 - 351 1998

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Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant Reviewed

T Hirayama, C Ishida, T Kuromori, S Obata, C Shimoda, M Yamamoto, K Shinozaki, C Ohto

FEBS LETTERS 413 ( 1 ) 16 - 20 1997.8

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DOI： 10.1016/S0014-5793(97)00871-5

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AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana Reviewed

T Hirayama, N Mitsukawa, D Shibata, K Shinozaki

PLANT MOLECULAR BIOLOGY 34 ( 1 ) 175 - 180 1997.5

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A cdc5(+) homolog of a higher plant, Arabidopsis thaliana Reviewed

T Hirayama, K Shinozaki

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 93 ( 23 ) 13371 - 13376 1996.11

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DOI： 10.1073/pnas.93.23.13371

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A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana Reviewed

T Mizoguchi, K Irie, T Hirayama, N Hayashida, K YamaguchiShinozaki, K Matsumoto, K Shinozaki

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 93 ( 2 ) 765 - 769 1996.1

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DOI： 10.1073/pnas.93.2.765

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A GENE ENCODING A PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C IS INDUCED BY DEHYDRATION AND SALT STRESS IN ARABIDOPSIS-THALIANA Reviewed

T HIRAYAMA, C OHTO, T MIZOGUCHI, K SHINOZAKI

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 92 ( 9 ) 3903 - 3907 1995.4

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DOI： 10.1073/pnas.92.9.3903

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Gene expression and signal transduction in Arabidopsis plants under water-stress conditions Reviewed

K Shinozaki, K YamaguchiShinozaki, T Urao, T Mizoguchi, T Hirayama, T Kiyosue, T Katagiri, M Noji, N Hayashida

PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL IV 511 - 516 1995

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Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae

Hirayarna, T., Maeda, T., Saito, H., Shinozaki, K.

Mgg Molecular & General Genetics 249 ( 2 ) 1995

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DOI： 10.1007/BF00290358

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A variety of functions of protein kinases in plants Reviewed

Mizoguchi, T., Hayashida, N., Hirayama, T., Urao, T., Iwasaki, T., Shinozaki, K.

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 39 ( 12 ) 2131 - 2149 1994

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NOVEL PROTEIN-KINASE OF ARABIDOPSIS-THALIANA (APK1) THAT PHOSPHORYLATES TYROSINE, SERINE AND THREONINE Reviewed

T HIRAYAMA, A OKA

PLANT MOLECULAR BIOLOGY 20 ( 4 ) 653 - 662 1992.11

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DOI： 10.1007/BF00046450

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EXON INTRON ORGANIZATION OF THE ARABIDOPSIS-THALIANA PROTEIN-KINASE GENES CDC2A AND CDC2B Reviewed

Y IMAJUKU, T HIRAYAMA, H ENDOH, A OKA

FEBS LETTERS 304 ( 1 ) 73 - 77 1992.6

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DOI： 10.1016/0014-5793(92)80592-5

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IDENTIFICATION OF 2 CELL-CYCLE-CONTROLLING CDC2 GENE HOMOLOGS IN ARABIDOPSIS-THALIANA Reviewed

T HIRAYAMA, Y IMAJUKU, T ANAI, M MATSUI, A OKA

GENE 105 ( 2 ) 159 - 165 1991.9

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DOI： 10.1016/0378-1119(91)90146-3

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CHARACTERIZATION OF THE VIR-A GENE OF THE AGROPINE-TYPE PLASMID PRIA4 OF AGROBACTERIUM-RHIZOGENES Reviewed

H ENDOH, T HIRAYAMA, T AOYAMA, A OKA

FEBS LETTERS 271 ( 1-2 ) 28 - 32 1990.10

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DOI： 10.1016/0014-5793(90)80364-O

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PUTATIVE START CODON TTG FOR THE REGULATORY PROTEIN VIRG OF THE HAIRY-ROOT-INDUCING PLASMID PRIA4 Reviewed

T AOYAMA, T HIRAYAMA, S TAMAMOTO, A OKA

GENE 78 ( 1 ) 173 - 178 1989.5

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DOI： 10.1016/0378-1119(89)90325-9

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ORGANIZATION AND CHARACTERIZATION OF THE VIRCD-GENES FROM AGROBACTERIUM-RHIZOGENES Reviewed

T HIRAYAMA, T MURANAKA, H OHKAWA, A OKA

MOLECULAR & GENERAL GENETICS 213 ( 2-3 ) 229 - 237 1988.8

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DOI： 10.1007/BF00339586

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成長過程の理解と農業形質予測 Invited Reviewed

平山隆志

アグリバイオ 8 34 - 38 2024.7

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アブシシン酸が働くための新たな仕組み Invited

西村宜之, 土屋渉, 平山隆志, 山崎俊正

化学と生物 57 736 - 742 2019.8

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Regulation of poly（A）status of plant organellar RNA Invited

Takashi Hirayama

Biological Science (Tokyo) 68 ( 4 ) 223 - 231 2017.5

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CiNii Article

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海藻は未知の植物ホルモン情報伝達機構を持つ

三上浩司, 森泉, 松浦恭和, 池田陽子, 平山隆志

Algal Resources 8 ( 1 ) 82 2015.7

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J-GLOBAL

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エチレン、ジベレリン、ジャスモン酸、ストリゴラクトン Invited

王敬銘, 伊藤晋作, 浅見忠男, 平山隆志, 中島正敏

化学と生物 48 5637 - 5642 2010.9

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アブシジン酸

上野琴巳, 平山隆志

化学と生物 48 ( 8 ) 555 - 563 2010.8

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DOI： 10.1271/kagakutoseibutsu.48.555

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多次元NMRと代謝物ケミカルシフトデータベースを用いたメタボローム一斉解析法の構築(Multidimensional NMR-based metabolomics using metabolite chemical shift databases)

近山英輔, 岡本真美, 関山恭代, 平山隆志, 斉藤和季, 菊地淳

生物物理 47 ( Suppl.1 ) S112 - S112 2007.11

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シロイヌナズナpolyA specific ribonucleaseの機能とホルモン応答

平山隆志, 鳴坂義弘, 安田美智子, 鳴坂真理, 北畑信隆, 黒森崇, 浅見忠男, 篠崎一雄, 仲下英雄, 西村宜之

日本植物生理学会年会要旨集 48th 80 2007.3

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DOI： 10.14841/jspp.2007.0.S039.0

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A New Pathosystem for Study on Plant-Fungal Pathogen Interaction.

Narusaka Yoshihiro, Enju Akiko, Sakurai Tetsuya, Satou Masakazu, Kobayashi Masatomo, Shinozaki Kazuo, Narusaka Mari, Park Pyoyun, Kubo Yasuyuki, Hirayama Takashi, Seki Motoaki, Shiraishi Tomonori, Ishida Junko, Nakashima Maiko

Plant and Cell Physiology Supplement 2004 ( 0 ) 454 - 454 2004.3

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When challenged with the fungus Colletotrichum higginsianum, Arabidopsis accession Columbia plant (Col-0) developed brown necrotic lesions, including a yellow halo around the lesion. The Arabidopsis accessions showed variation in response to C. higginsianum. Arabidopsis accession Eil-0 was highly resistant to C. higginsianum. In the Eil-0 plant inoculated with the fungus, cell death did not appear as a rapid response, and the level of camalexin accumulated was low. Analyses using RNA gel blotting and cDNA microarray suggested that the defense responses against C. higginsianum occur mainly through the JA/ET-dependent pathway. Segregation analysis of progeny from crosses between Eil-0 and Col-0 plants was performed to determine the genetic basis of resistance. The resistance was dominant and seemed to be conferred by a single locus. Arabidopsis and C. higginsianum provide an excellent pathosystem for studying the molecular and cellular bases of plant-pathogen interactions

DOI： 10.14841/jspp.2004.0.454.0

CiNii Article

J-GLOBAL

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The 10th Arabidopsis Meeting 参加報告

平山隆志, Kim Gyung-Tae, 関原明, 篠崎一雄

植物の化学調節 34 ( 2 ) 316 - 320 1999.12

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真核生物の細胞周期調節に関与する新たなMyb様転写因子植物, 酵母からヒトまで広く存在するCdc5.標的遺伝子の解明が急務

平山隆志, 篠崎一雄

化学と生物 36 ( 1 ) 2 - 4 1998.1

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プロテインキナ-ゼ (植物のシグナルトランスダクション--分子機構と実験法) -- (タンパク質のリン酸化と第二メッセンジャ-)

溝口剛, 篠崎一雄, 平山隆志

現代化学増刊 ( 30 ) 130 - 168 1996.11

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Receptors for plant hormones

HIRAYAMA Takashi, SHINOZAKI Kazuo

Chemical Regulation of Plants 30 ( 2 ) 144 - 149 1995.12

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Presentations

原始紅藻 ‘Bangia’ sp. ESS1が持つ高度な海水希釈ストレス耐性は細胞形態と脂肪酸組成の可逆的変化を伴う

Puja Kumari, 松島良, 平山隆志, 三上浩司

日本応用藻類学会第22回大会 2024

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Event date： 2024.9.7 - 2024.9.8

Language：Japanese Presentation type：Oral presentation (general)

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Field transcriptome analysis unveils novel genetic pathways for early heading in spring barley

日本植物生理学会年会(Web) 2024

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Dissection of developmental state transition in the shoot apical meristem of barley by single meristem RNA-seq

井藤純, 野村有子, 高萩航太郎, KIM J., KIM J., 鹿島誠, 岡田聡史, 佐藤奈緒, 清水みなみ, 最相大輔, 持田恵一, 平山隆志, 辻寛之, 辻寛之

育種学研究 2023

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Developmental state transition in the shoot apical meristem of barley

井藤純, 野村有子, 高萩航太郎, 岡田聡史, 久下修平, 佐藤奈緒, 新井駿一, 松本大輝, 杉村みどり, 関緑, 服部公央亮, 梅崎太造, 池田陽子, 最相大輔, 持田恵一, 平山隆志, 辻寛之

日本植物生理学会年会(Web) 2022

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Gene expression analysis of the unique inflorescence structure in barley

井藤純, 佐藤奈緒, 野村有子, 新井駿一, 高萩航太郎, 岡田聡史, 岡田聡史, 武田(神谷)紀子, 豊岡公徳, 最相大輔, 平山隆志, 持田恵一, 辻寛之, 辻寛之

育種学研究 2022

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Data driven crop design technology

平山隆志, 最相大輔, 井藤純, 服部公央亮, 岡田聡史, 池田陽子, 梅崎太造, 辻寛之, 持田恵一

日本植物生理学会年会(Web) 2022

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Language：Japanese Presentation type：Poster presentation

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Data driven crop design technology

平山隆志, 岡田聡史, 最相大輔, 井藤純, 服部公央亮, 池田陽子, 梅崎太造, 辻寛之, 持田恵一

日本分子生物学会年会プログラム・要旨集(Web) 2021

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Genomic research across the barley life cycle toward ’preemptive breeding’

最相大輔, 岡田聡史, 金谷麻加, 金谷麻加, 池田陽子, 井藤純, 辻寛之, 持田恵一, 持田恵一, 持田恵一, 平山隆志

育種学研究 2021

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Developmental trajectory analysis of the shoot apex in barley by single meristem transcriptome

井藤純, 野村有子, 佐藤奈緒, 岡田聡史, 高萩航太郎, 杉村みどり, 関緑, 最相大輔, 持田恵一, 平山隆志, 辻寛之

育種学研究 2021

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Pseudo-time transition of barley life cycle in the field

最相大輔, 岡田聡史, 金谷麻加, 金谷麻加, 池田陽子, 井藤純, 辻寛之, 持田恵一, 持田恵一, 持田恵一, 平山隆志

育種学研究 2021

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Developmental trajectory analysis of barley life cycle using field transcriptome data

岡田聡史, 最相大輔, 金谷麻加, 金谷麻加, 池田陽子, 井藤純, 辻寛之, 井上小槙, 上原由紀子, 清水みなみ, 持田恵一, 持田恵一, 持田恵一, 平山隆志

育種学研究 2021

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イメージングから探るオオムギシュート頂メリステムの発生過程

井藤純, 久下修平, 新井駿一, 佐藤奈緒, 鷲見典克, 服部公央亮, 野村有子, 赤司裕子, 田中真理, 最相大輔, 梅崎太造, 平山隆志, 辻寛之

日本植物学会大会研究発表記録(CD-ROM) 2020

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Intraspecific variation of field growth dynamics in barley

最相大輔, 岡田聡史, 井藤純, 辻寛之, 高萩航太郎, 高萩航太郎, 持田恵一, 持田恵一, 持田恵一, 平山隆志

育種学研究 2020

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Genetic analysis on heading time of barley (Hordeum vulgare) under multiple environments

岡田聡史, 最相大輔, 井藤純, 辻寛之, 高萩航太郎, 持田恵一, 持田恵一, 平山隆志

育種学研究 2020

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ゼニゴケの新規DNAメチル化制御機構:植物と動物の狭間で

池田陽子, 十川太輔, 西浜竜一, 山岡尚平, 荒木崇, 河内孝之, 平山隆志, 大和勝幸

日本植物学会大会研究発表記録(CD-ROM) 2020

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圃場オオムギを用いた時系列クロマチン修飾解析

池田陽子, 金谷麻加, 井上小槙, 最相大輔, 井藤純, 辻寛之, 持田恵一, 持田恵一, 持田恵一, 平山隆志

日本植物生理学会年会(Web) 2019

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野外環境におけるオオムギ茎頂メリステムの成長過程の系統間比較

井藤純, 野村有子, 最相大輔, 高萩航太郎, 高萩航太郎, 持田恵一, 持田恵一, 平山隆志, 辻寛之

育種学研究 2019

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任意の圃場環境に最適な作物の育種を可能に-データ科学に基づく作物設計基盤技術の開発

平山隆志, 最相大輔, 井藤純, 高萩航太郎, 香西雄介, 鷲見典克, 池田陽子, 井上小槙, 上原由紀子, 清水みなみ, 服部公央亮, 梅崎太造, 梅崎太造, 辻寛之, 持田恵一, 持田恵一, 持田恵一

日本生物工学会大会講演要旨集 2019

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葉身伸長に基づくオオムギ生長過程の表現型可塑性

最相大輔, 井藤純, 辻寛之, 平山隆志

育種学研究 2018

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フィールド環境下のオオムギの花芽形成過程における茎頂メリステムの応答性の系統間差

井藤純, 野村有子, 最相大輔, 平山隆志, 辻寛之

日本植物生理学会年会(Web) 2018

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圃場環境下における野生オオムギと栽培オオムギの生長段階と植物ホルモン動態の季節変動

最相大輔, 松浦恭和, 池田陽子, 森泉, 持田恵一, 持田恵一, 平山隆志

育種学研究 2017

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Analysis of a PARN deficient mutant, ABA hypersensitive germination2-1, of Arabidopsis.

Hirayama, T, Ushiyama, S, Hayashi S, Matsuura, T, Mori, I

The 16th Annual Meeting of the RNA Society and The 13th Annual Meeting of the RNA Society of Japan. 2011.6.16

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Event date： 2011.6.14 - 2011.6.18

Language：English Presentation type：Poster presentation

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シロイヌナズナpolyA specific ribonucleaseの機能とホルモン応答

平山隆志, 鳴坂義弘, 安田美智子, 鳴坂真理, 北畑信隆, 黒森崇, 浅見忠男, 篠崎一雄, 仲下英雄, 西村宜之

日本植物生理学会年会要旨集 2007.3.15

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Language：Japanese

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Function of PolyA specific ribonuclease in plant hormone response

Hirayama Takashi, Narusaka Yoshihiro, Yasuda Michiko, Narusaka Mari, Kitahata Nobutaka, Kuromori Takashi, Asami Tadao, Shinozaki Kazuo, Nakashita Hideo, Nishimura Noriyuki

PLANT AND CELL PHYSIOLOGY 2007

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Event date： 2007

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多次元NMRメタボロミクスの基盤整備と植物代謝物解析への応用

田春杰, 坪井裕理, 近山英輔, 関山恭代, 黒森崇, 篠崎一雄, 平山隆志, 菊地淳

日本植物生理学会年会要旨集 2006.3 The Japanese Society of Plant Physiologists

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Language：Japanese

In these days of post genome era, metabolome studies attract more attention because the information on metabolites is the key data for so-called systems biology. To analyze the metabolites, mass spectrometry (MS) has been used mainly. However, the MS analysis is not always suitable to detect chemically-diversed metabolites. Therefore, we have been trying to establish NMR-based metabolomics approach. We have conducted the stable isotope labeling of plants, insects and mice, optimization of the extraction solvent for metabolites, building a chemical shift database, and constructing a semi-automtic signal assignment system, SpinAssign. In this talk, we will review these methodologic developments. We will also show the in vivo NMR measurements of Arabidopsis T87 cultured cells treated with ABA coupled with transcriptome analysis, and our attempt to detect metabolite changes in Ds insertion mutants. In addition, the future perspective and the potential of NMR metabolomics will be discussed.

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シロイヌナズナの概日リズム制御と光周性花成

藤原すみれ, 小田篤, 田島武臣, CALVINO M, 大越友里, 黒森崇, 平山隆志, 楠城時彦, 篠崎一雄

日本植物生理学会年会要旨集 2004.3.20

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Language：Japanese

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A New Pathosystem for Study on Plant-Fungal Pathogen Interaction.

Narusaka Yoshihiro, Enju Akiko, Sakurai Tetsuya, Satou Masakazu, Kobayashi Masatomo, Shinozaki Kazuo, Narusaka Mari, Park Pyoyun, Kubo Yasuyuki, Hirayama Takashi, Seki Motoaki, Shiraishi Tomonori, Ishida Junko, Nakashima Maiko

Plant and Cell Physiology Supplement 2004.3.20 日本植物生理学会

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Event date： 2004.3.20

When challenged with the fungus Colletotrichum higginsianum, Arabidopsis accession Columbia plant (Col-0) developed brown necrotic lesions, including a yellow halo around the lesion. The Arabidopsis accessions showed variation in response to C. higginsianum. Arabidopsis accession Eil-0 was highly resistant to C. higginsianum. In the Eil-0 plant inoculated with the fungus, cell death did not appear as a rapid response, and the level of camalexin accumulated was low. Analyses using RNA gel blotting and cDNA microarray suggested that the defense responses against C. higginsianum occur mainly through the JA/ET-dependent pathway. Segregation analysis of progeny from crosses between Eil-0 and Col-0 plants was performed to determine the genetic basis of resistance. The resistance was dominant and seemed to be conferred by a single locus. Arabidopsis and C. higginsianum provide an excellent pathosystem for studying the molecular and cellular bases of plant-pathogen interactions

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Characterization and functional analyses of DOG1-dependent ABA signaling cascade

Noriyuki Nishimura, Wataru Tsuchiya, Ryoichi Yano, Nahomi Suzuki, Takashi Hirayama, Toshimasa Yamazaki

2024 International Conference on Arabidopsis Research 2024.7.17

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Polyadenylation negatively regulates cytochrome c maturation through the modulation of C-to-U editing of ccb3/ccmC mRNA in plant mitochondria

Akihito Mamiya, Kayoko Yamamoto, Takehito Kobayashi, Yusuke Yagi, Takahiro Nakamura, Hidehiro Fukaki, Issei Nakazato, Shin-ichi Arimura, Takashi Hirayama, Munetaka Sugiyama

13th International Conference for Plant Mitochondrial Biology 2024.5.28

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Uncovering the link between RNA editing and polyadenylation, two mysterious modifications of plant mitochondrial mRNA Invited

Akihito Mamiya, Kayoko Yamamoto, Takehito Kobayashi, Yusuke Yagi, Takahiro Nakamura, Hidehiro Fukaki, June-Sik Kim, Issei Nakazato, Shin-ichi Arimura, Munetaka Sugiyama, Takashi Hirayama

2024.3.19

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圃場作物ライフコースホルモン一斉分析で見えてきたもの Invited

平山隆志

日本植物生理学会年会関連集会 2024.3.19

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Analysis of AGS2 RNA helicase implicated in the post-transcriptional regulation of mitochondria mRNA

Haruko Kaita, June-Sik Kim, Akihito Mamiya, Munetaka Sugiyama, Keiichi Mochida, Takashi Hirayama

2024.3.18

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Arabidopsis SD5/DROL1 protein, a subunit of U5 snRNP, regulates nutrient response through pre-mRNA splicing and jasmonic acid signaling

Kodai Ishibashi, Toshihiro Arae, Takeshi Yoshizumi, Yukio Kurihara, Takashi Kuromori, Zofia Szweykowska-Kulińska, Artur Jarmołowski, Takashi Hirayama, Minami Matsui, Misato Ohtani

2024.3.18

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ミトコンドリアのpolyA付加mRNA蓄積の影響を抑制するags2変異

平山隆志

第1回植物RNA研究者ネットワークシンポジウム 2023.12.10

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植物ミトコンドリアmRNAのポリA付加はシトクロムc成熟因子ccb3/ccmCのmRNA編集抑制によりシトクロム呼吸経路を負に制御する

間宮章仁, 山本荷葉子, 小林健人, 八木祐介, 中村崇裕, 平山隆志, 深城英弘, 杉山宗隆

日本分子生物学会 2023.12.8

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Photosynthetic activity regulates lateral root morphogenesis via pre-mRNA splicing

Takayanagi, N, Arae, T, Shimizu, T, Horiguchi, G, Aida, M, Fukaki, H. Masuda, T, Notaguchi, M, Hirayama, T, Ohtani, M

TJPB2023 2023.10.15

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Life course monitoring of plant hormones of barleys grown in the field conditions

Hirayama, T, Saisho, D, Kim, J.S, Okada, S, Ito, J, Matsuura, T, Hattori, K, Tsuji, H, Koda, S, Nishii, R, Umezaki, T, Mochida, K

The 3rd Barley Mutant Conference 2023.10.9

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Dissection of developmental state transition in the shoot apical meristem of barley by single meristem RNA-seq

Ito, J, Nomura, Y, Takahagi, K, Kim, J.S, Kashima, M, Okada, S, Sato, N, Shimizu, M, Saisho, D, Mochida, K, Hirayama, T, Tsuji, H

The 3rd Barley Mutant Conference 2023.10.9

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Functional analysis of DOG1-dependent ABA signaling cascade

Nishimura, N, Tsuchiya, W, Suzuki, N, Hirayama, T, Yamazaki, T

15th International Symposium on Pre-Harvest Sprouting in Cereals 2023.10.6

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シングルメリステムRNA-seq解析によるオオムギ茎頂メリステムの発生ステート推移の解明

井藤純, 野村有子, 高萩航太郎, 金俊植, 鹿島誠, 岡田聡史, 佐藤奈緒, 清水みなみ, 最相大輔, 持田恵一, 平山隆志, 辻寛之

日本育種学会第144回講演会 2023.9.18

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Functional analysis of DOG1-dependent ABA signaling cascade

Nishimura, N, Tsuchiya, W, Suzuki, N, Hirayama, T, Yamazaki, T

The 33rd International Conference on Arabidopsis Research 2023.6.8

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Field transcriptome dynamics of barley during winter cultivation.

Kim. J.S, Ito, J, Takahagi, K, Kanatani, A, Shimizu, M, Uehara-Yamaguchi, Y, Inoue, K, Okada, S, Matsuura, T, Hattori, K, Ikeda, Y, Saisho, D, Tsuji, H, Hirayama, T, Sato, K, Mochida, K

The 33rd International Conference on Arabidopsis Research 2023.6.8

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Role of pre-mRNA splicing in lateral root morphogenesis regulated by plastid signal.

Takayanagi, N, Arae, T, Shimizu, T, Horiguchi, G, Aida, M, Fukaki, H. Masuda, T, Notaguchi, M, Hirayama, T, Ohtani, M

The 33rd International Conference on Arabidopsis Research 2023.6.8

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A defect in an RNA metabolic enzyme suppresses the adverse effect of the accumulation of polyadenylated mitochondrial mRNA in Arabidopsis

Hirayama, T, Kim. J.S, Sorokin, A, Mochida, K

The 33rd International Conference on Arabidopsis Research 2023.6.7

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Interaction between polyadenylation and C-to-U editing of mitochondrial mRNA involved in cytochrome c maturation.

Mamiya, A, Yamamoto, K, Kobayashi, Y, Yagi, Y, Nakamura, T, Hirayama, T, Fukaki, H, Sugiyama, M

The 33rd International Conference on Arabidopsis Research 2023.6.6

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植物ミトコンドリアのシトクロムc成熟に関わるmRNA編集とポリA付加の関係性について

間宮章仁, 山本荷葉子, 小林健人, 八木祐介, 中村崇裕, 平山隆志, 深城英弘, 杉山宗隆

日本植物生理学会年会 2023.3.18

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ミトコンドリアにおけるpolyA付加mRNAの蓄積の影響を抑制するシロイヌナズナの変異について

平山隆志, 金俊植, 持田恵一

日本植物生理学会年会 2023.3.18

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オオムギ匍匐性は作用が異なる3つのQTLが制御する複合形質である

福嶋七海, 松浦恭和, 門田有希, 西田英隆, 平山隆志, 加藤鎌司

日本育種学会第142回大会 2022.9.24

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植物ミトコンドリアにおけるシトクロムC成熟に関わるmRNA編集とポリA付加の関係性について

間宮章仁, 大塚蔵嵩, 山本荷葉子, 小林健人, 八木祐介, 中村崇裕, 平山隆志, 深城英弘, 杉山宗隆

日本植物学会 2022.9.17

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Interaction between polyadenylation and C-to-U editing of mitochondrial mRNA involved in cytochorome c maturation

Mamiya, A, Otsuka, K, Kobayashi, T, Yagi, Y, Nakamura, T, Hirayama, T, Fukaki, H, Sugiyama, M

12th International Conference for Plant Mitochondrial Biology 2022.5.25

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シングルメリステムRNA-seq解析によるオオムギ茎頂メリステムの発生ステート推移の解明

井藤純, 野村有子, 高萩航太郎, 岡田聡史, 佐藤奈緒, 松本大輝, 新井駿一, 杉村みどり, 関緑, 服部公央亮, 梅崎太造, 最相大輔, 持田恵一, 平山隆志, 辻寛之

日本植物生理学会年会 2022.3.24

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ディープフェノタイピングによるオオムギの成長トラジェクトリの多様性の解明

持田恵一, 金俊植, 高萩航太郎, 金谷麻加, 井上小槙, 上原由紀子, 清水みなみ, 最相大輔, 井藤純, 服部公央亮, 岡田聡史, 池田陽子, 梅崎太造, 辻寛之, 平山隆志

日本植物生理学会年会 2022.3.23

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Diversity of FEP/IMA genes across higher plants

Sorokin, A, Hirayama, T

2022.3.23

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A genome-wide chronological study of histone modifications and gene expression in barley grown under field conditions

Ikeda, Y, Okada, S, Kanatani, K, Inoue, K, Saisho, D, Ito, J, Tsuji, H, Mochida, K, Hirayama, T

2021.3.15

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Life-course phytohormone profiling of field barley for seasons reveals unique fluctuations along with environmental responses and developmental progress under the field conditions

Hirayama, T, Saisho, D, Matsuura, T, Ito, J, Okada, S, Kanatani, A, Tsuji, H, Mochida, K

2021.3.15

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Diversity of physiological states in diverse barley accessions under field conditions.

Hirayama T, Takahagi, K, Inoue, K, Uehara-Yamaguchi, Y, Kanatani, A, Saisho, D, Matsuura, T, Ito, J, Matsushita, Y, Tsuji, H, Mochida, K

2020.3.20

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Diversity of developmental trajectory in barley under field conditions

Mochida, K, Takahagi, K, Inoue, K, Uehara-Yamaguchi, Y, Shimizu, M, Kanatani, A, Saisho, D, Matsushita, Y, Hirayama T

2020.3.20

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A regulatory system of seed dormancy and germination regulated by abscisic acid signaling

Nishimura, N, Tsuchiya, W, Moresco, J.J, Hayashi, Y, Satoh, K, Kaiwa, N, Irisa, T, Kinoshita, T, Schroeder, J.I, Yates, J.R, Hirayama, T, Yamazaki, T

6th Workshop on the Molecular Aspects of Seed Dormancy and Germination 2019.9.23

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任意の圃場環境に最適な作物の育種を可能に～データ科学に基づく作物設計基盤技術の開発～ Invited

平山隆志

日本生物工学会 2019.9.17

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ミトコンドリア電子伝達系の不具合が引き起こす，側根形成時の過剰な細胞分裂について

間宮章仁, 大塚蔵嵩, 小林健人, 八木祐介, 中村崇裕, 平山隆志, 杉山宗隆

日本植物学会 2019.9.16

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Mask R-CNN を用いたオオムギの葉に関する形態的特徴の抽出

加藤有祐, 鷲見典克, 服部公央亮, 田口亮, 保黒政大, 最相大輔, 梅崎太造, 平山隆志

画像センシング展2019 2019.6.13

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Analysis of mitochondrial RNA processing factors involved in lateral root development

Mamiya, A, Otsuka, K, Kobayashi, K, Yagi, Y, Nakamura, T, Hirayama, T, Sugiyama, M

Post-transcriptional Gene Regulation in Plants 2019 Nara 2019.3.18

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Regulation of the poly(A) status of mitochondrial mRNA is conserved among land plants

Kanazawa, M, Ikeda, Y, Nishihama, R, Yamaoka, R, Yamato, K.T, Kohchi, T, Hirayama, T

Post-transcriptional Gene Regulation in Plants 2019 Nara 2019.3.18

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Variation of gene regulatory networks of flowering in barley under field conditions

Inoue, K, Takahagi, K, Uhehara-Yamaguchi, Y, Shimizu, M, Saisho, D, Matsuura, T, Kanatani, A, Ito, J, Tsuji, H, Hirayama, T, Mochida, K

2019.3.14

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Combined hormonome and transcriptome profiling of barley throughout the life-course under field conditions reveals conserved, genotype- and life-stage specific physiological states

Hirayama, T, Saisho, D, Takahagi, K, Matsuura, T, Kanatani, A, Inoue, K, Uehara-Yamaguchi, Y, Shimizu, M, Mochida, K

2019.3.14

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Chronological Analysis of chromatin modification using barley grown under field conditions

keda, Y, Kanatani, A, Inoue, K, Saisho, D, Ito, J, Tsuji, H, Mochida, K, Hirayama, T

2019.3.14

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Physiological dynamics across diverse barley accessions under filed condition illustrated by combined hormonome and transcriptome profiling

Hirayama, T, Saisho, D, Takahagi, K, Matuura, T, Kanatani, A, Inoue, K, Uehara-Yamaguchi, Y, Shimizu, M, Mochida, K

Phenome 2019 2019.2.8

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Phenotypic plasticity of barley developmental trajectory

Saisho, D, Ito, J, Tsuji, H, Mochida, K, Ikeda, Y, Hirayama, T

Phenome 2019 2019.2.8

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野外環境におけるオオムギ茎頂メリステムの成長過程の系統間比較

井藤純, 野村有子, 最相大輔, 持田恵一, 平山隆志, 辻寛之

第13回ムギ類研究会 2018.11.27

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オオムギ生長過程の表現型可塑性

最相大輔, 井藤純, 辻寛之, 持田恵一, 池田陽子, 平山隆志

第13回ムギ類研究会 2018.11.26

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オオムギ茎頂メリステムの発生フェノロジー.第13回ムギ類研究会

井藤純, 野村有子, 最相大輔, 平山隆志, 辻寛之

第13回ムギ類研究会 2018.11.26

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Control of seed dormancy and germination by DOG1-AHG1 PP2C Phosphatase complex via binding to heme.

Nishimura, N, Tsuchiya, W, Moresco, J.J, Hayashi, Y, Satoh, K, Kaiwa, N, Irisa, T, Kinoshita, T, Schroeder, J. I, Yates III, J. R, Hirayama, T, Yamazaki, T

6th Plant Dormancy Symposium 2018 2018.10.25

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水稲品種Khao Nam Jenに由来する日長非依存的な出穂期QTLの検出

堀清純, 永田和史, 福岡修一, 最相大輔, 平山隆志, 持田恵一

日本育種学会第134回講演会 2018.9.22

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DNNを用いたオオムギ領域の抽出および生長推定への活用

加藤有祐, 鷲見典克, 服部公央亮, 田口亮, 保黒政大, 最相大輔, 梅崎太造, 平山隆志

第17回情報科学技術フォーラム 2018.9.21

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IR画像のセグメンテーションによるオオムギの生長度合いの推定

松井悠, 鷲見典克, 服部公央亮, 田口亮, 保黒政大, 最相大輔, 梅崎太造, 平山隆志

第17回情報科学技術フォーラム 2018.9.20

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ゼニゴケにおけるDNAメチル化制御.

池田陽子, 西浜竜一, 山岡尚平, Arteaga-Vazquez, Mario A, Grimanelli, Daniel・Martienssen, Robert A・Pogorelcnik, Romain・Mathieu, Olivier, 大和勝幸, 河内孝之, 平山隆志

日本植物学会 2018.9.15

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A Novel short Peptide regulates the iron-deficient responsive genes in Arabidopsis.

Hirayama, T, Lei G. J, Nakagawa, N, Yamaji, N, Ma J. F

29th International Conference on Arabidopsis Research. 2018.6.26

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Field multi-omics approaches in barley toward robust crop development

Saisho, D, Ito, J, Tsuji, H, Mochida, K, Ikeda, Y, Hirayama, T

Second International Barley Mutants Workshop 2018 2018.6.26

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Plasticity of flowering time in response to environmental cues at the shoot apex in field-grown barley varieties Invited

Ito, J, Saisho, D, Mochida, K, Hirayama, T, Tsuji, H

25th International Conference on Sexual Plant Reproduction. 2018.6.12

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野外トランスクリプトームデータ解析によるオオムギ成長ステージの遷移およびその多様性の解明

高萩航太郎, 松下康弘, 井上小槙, 山口(上原, 由紀子, 最相大輔, 江田智尊, 西井龍映, 平山隆志, 持田恵一

日本植物生理学会年会 2018.3.30

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発達中の小麦種子の植物ホルモンの一斉分析

松浦恭和, 森泉, 平山隆志

日本農芸化学会 2018.3.29

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An integrated genome information resources in Pooideae and its application to identify homoeologous relations of transcription factors in barley and wheat

Inoue, K, Takahagi, K, Hirayama, T, Mochida

2018.3.29

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Field crop data acquisition for the data driven crop design technology

Saisho, D, Sumi, N, Hattori, K, Ikeda, Y, Mochida, K, Umezaki, T, Hirayama

2018.3.29

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Functional data analysis applied to identify time-series changes of environmental factors that are related to flowering in barley.

Mochida, K, Matsui, H, Lipka, A. E, Takahagi, K, Inoue, K, Ishii, M, Sato. K, Hirayama, T

2018.3.28

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Field multi-omics approaches in barley to reveal crop phenology.

Ikeda, Y, Saisho, D, Matsuura, T, Ito, J, Tsuji, H, Mochida, K, Hirayama, T

2018.3.28

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IR画像のセグメンテーションによるオオムギの生長度合いの推定.

松井悠, 鷲見典克, 服部公央亮, 田口亮, 保黒政大, 最相大輔, 梅崎太造, 平山隆志

動的画像処理実利用化ワークショップ2018 2018.3.8

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セマンティックセグメンテーションによるオオムギ領域の抽出

加藤有祐, 鷲見典克, 服部公央亮, 田口亮, 保黒政大, 最相大輔, 梅崎太造, 平山隆志

動的画像処理実利用化ワークショップ2018 2018.3.8

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Field omics approach to explore physiological variations decisive for agricultural traits Invited

Hirayama. T

Workshop：Data driven crop design technology, 2018.2.20

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多視点カメラを用いたオオムギ生長度合いの推定

鷲見典克, 服部公央亮, 最相大輔, 田口亮, 保黒政大, 平山隆志, 梅崎太造

Vision Engineering Workshop2017 2017.12.7

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Data driven crop design technology

Hirayama, T, Saisho, D, Ikeda, Y, Sumi, N, Hattori, K, Hiroyuki, T, Umezaki, T, Mochida, K

Taiwan-Japan Plant Biology 2017 2017.11.5

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シロイヌナズナの側根形成初期過程において高温下で過剰な細胞分裂をもたらすミトコンドリア異常の解析

間宮章仁, 大塚蔵嵩, 野崎守, 山本荷葉子, 小林健人, 八木祐介, 中村崇裕, 平山隆志, 上田貴志, 蜂谷卓士, 野口航, 杉山宗隆

日本植物学会 2017.9.9

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ゼニゴケのPARNはミトコンドリアmRNAポリA鎖長を制御する

金澤まい, 池田陽子, 西浜竜一, 山岡尚平, 大和勝幸, 河内孝之, 平山隆志

第19回日本RNA学会年会 2017.7.20

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圃場環境下における野生オオムギと栽培オオムギの生長段階と植物ホルモン動態の季節変動

最相大輔, 松浦恭和, 池田陽子, 森泉, 持田恵一, 平山隆志

日本育種学会第131回講演会 2017.3.29

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Unlocking physiological dynamics throughout crop lifecycles under field condition

Mochida, K, Takahagi, K, Matsuura, T, Ikeda, Y, Saisho, D, Hirayama, T

2nd Agriculture and Climate Change Conference 2017.3.27

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A novel peptide involved in the iron homeostasis under a mitochondrial defect in Arabidopsis

Hirayama, T, Lei, G.J, Ma, J.F

2017.3.17

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ゼニゴケミトコンドリアmRNAポリA鎖長制御因子MpAHG2及びMpAGS1の解析

金澤まい, 池田陽子, 西浜竜一, 山岡尚平, 大和勝幸, 河内孝之, 平山隆志

日本植物生理学会年会 2017.3.17

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Identification and characterization of AHG1-mediated ABA signaling components in Arabidopsis

Nishimura, N, Tsuchiya, W, Moresco, J.J, Satoh, K, Kaiwa, K, Irisa, T, Hayashi, Y, Kinoshita, T, Schroeder, J.I, Yates, J.R.III, Hirayama, T, Yamazaki, T

2017.3.16

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PARN directly regulates the poly(A) status of mitochondrial mRNA in liverwort.

Kanazawa, M, Ikeda, Y, Nishihama, R, Yamaoka, S, Kohchi, T, Hirayama, T

2016.12.1

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オルガネラ機能と植物ホルモン等情報分子の絡まり Invited

平山隆志

日本植物学会 2016.9.17

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シロイヌナズナの側根形成時に細胞分裂の制限に関わるミトコンドリアmRNA 編集・ポリA代謝の解析

間宮章仁, 野崎守, 大塚蔵嵩, 山本荷葉子, 小林健人, 八木祐介, 中村崇裕, 平山隆志, 杉山宗隆

日本植物学会 2016.9.16

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Empirical identification of the transcriptional network for H2O2 responses in Arabidopsis

Hieno, A, Hshuna N.A, Hasegawa-Inaba, K, Yokogawa, T, Obata. D, Nomoto, M, Tada. Y, Yokogawa, T, Higuchi, M, Hanada, K, Matsui, M, Hirayama, T, Mitsuda, N, Yamamoto Y

ICES 2016 Kyoto 2016.9.12

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Poly(A)-dependent metabolism and editing of mitochondrial RNA are involved in the OFF regulation of formative cell division during lateral root organogenesis in Arabidopsis thaliana.

Mamiya, A, Otsuka, K, Yamamoto, K, Nozaki, M, Yagi, Y, Nakamura, T, Ueda, T, Hirayama,T, Sugiyama, M

The 21st Annual Meeting of the RNA Society 2016.7.30

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PARN and PAP regulate the poly(A) status of mitochondrial mRNA in liverwort.

Kanazawa, M, Ikeda, Y, Nishihama, R, Yamaoka, S, Kohchi, T, Hirayama, T

The 21st Annual Meeting of the RNA Society 2016.6.30

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Transcriptome anaylsis of ahg2-1 revealed a unique relation between mitochondrial and cellular functions.

Hirayama, T

2016.3.20

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ゼニゴケミトコンドリアmRNAポリA尾部長制御因子の解析

金澤まい, 池田陽子, 西浜竜一, 山岡尚平, 河内孝之, 平山隆志

日本植物学会 2016.3.19

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A complex ABA signaling network mediated by PP2Cs

Nishimura, N, Moresco, J, Mitsuda, N, Tu, P, Nishimura, H, Hayashi, Y, Hirayama, T, Kinoshita, T, Schroeder, J.I, Yates, J.R, Satoh, K

2016.3.18

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シロイヌナズナの側根原基形成における非対称細胞分裂の終結制御とミトコンドリア機能および温度との関係

間宮章仁, 大塚蔵嵩, 山本荷葉子, 八木祐介, 中村崇裕, 野崎守, 佐藤康, 上田貴志, 蜂谷卓士, 野口航, 平山隆志, 杉山宗隆

日本分子生物学会 2015.12.4

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褐藻シオミドロ雄配偶体に優先的なサイトカイニンの蓄積

三上浩司, 森泉, 池田陽子, 松浦恭和, 平山隆志

日本水産学会秋季大会 2015.9.24

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The purine metabolite allantoin can activate the MYC2-modulated JA-signaling pathway in an ABA-dependent manner.

Takagi, H, Ishiga, Y, Egusa, M, Watanabe, S, Konishi, T, Akiyoshi, N, Matsuura, T, Mori, I, Hirayama, T, Shimada, H, Kaminaka, H, Sakamoto, A

Plant Biology 2015 2015.7.27

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海藻は未知の植物ホルモン情報伝達機構を持つ

三上浩司, 森泉, 松浦恭和, 池田陽子, 平山隆志

日本応用藻類学会第14回大会 2015.5.16

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アブシジン酸シグナルで働くAHG1の相互作用因子の解析

西村宜之, James Moresco, 光田展隆, Patricia Tu, 西村秀希, 林優紀, 平山隆志, 木下俊則, Julian I. Schroeder, John R. Yates, III, 佐藤浩二

日本植物生理学会年会 2015.3.18

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Isolation of interacting proteins for AHG2 or AGS1 that is involved in the regulation of poly(A) status of mitochondrial mRNA in plants

Hirayama, T, Nakagawa, R, Yagi, Y, Ma, J.F, Nakamura, T, Matsuura, T

2015.3.17

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シロイヌナズナの側根帯化変異体の解析から見えてきたミトコンドリアmRNAのポリA依存的代謝と編集の機能的連関

大塚蔵嵩, 山本荷葉子, 間宮章仁, 斉藤真人, 有田真規, 玉置裕章, 八木祐介, 中村崇裕, 野崎守, 佐藤康, 上田貴志, 平山隆志, 杉山宗隆

日本植物生理学会年会 2015.3.17

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ゼニゴケEILがエチレンおよび硫黄栄養応答に果たす役割

牧野宏美, 前田祐華, 上土井優貴, 陶山明子, 石田咲子, 石崎公庸, 西浜竜一, 平山隆志, 河内孝之, 丸山明子

日本植物生理学会年会 2015.3.17

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シロイヌナズナPARN変異株ahg2のミトコンドリアトランスクリプトーム解析. 第４回植物RNA研究者ネットワークシンポジウム

平山隆志

第４回植物RNA研究者ネットワークシンポジウム 2015.1.19

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原始紅藻類における植物ホルモン定量分析法の開発

三上浩司, 森泉, 松浦恭和, 平山隆志

日本水産学会秋季大会 2014.9.21

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Unique function of poly(A)-specific ribonuclease of Arabidopsis

Hirayama, T, Matsuura, T, Nakagawa, R, Ohtani, M, Hayashi, H

International Conference on Arabidopsis Research 2014.7.30

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Abscisic acid induces ectopic outgrowth and cortical microtubule reorganization in epidermal cells of Arabidopsis thalianaa

Takatani, S, Hirayama, T, Hashimoto, T, Takahashi, T, Motose, H

International Conference on Arabidopsis Research 2014.7.29

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Transcriptome analysis reveals the role of plant hormones in pear fruit set

Itai, A, Morishita, Y, MIshima, R, Kanetaka, S, Matsuura, T, Mori, I, Hirayama, T, Murayama, H, Habu, T

12th International Pear Symposium 2014.7.16

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Study for mechanism of the disease resistance in the transgenic rice accumulated β-amyrin

Nishira T, Nakatsuji Y, Kusunoki K, Ikeda K, Goto Y, Mano A, Osbourn A, Matsuura T, Mori I C, Hirayama T, Noutoshi Y, Toyoda K, Shiraishi T, Yamamoto M, Ichinose Y, Inagaki Y

2014.7.3

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ABI1, a negative regulator of abscisic acid signalling, regulates plant growth in response to balance of carbon and nitrogen availability in Arabidopsis

Lu, Y, Sato, T, Mori, I, Hirayama, T, Yamaguchi, J

2014.3.20

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poly(A)特異的RNA分解酵素(PARN)の植物特有の機能

平山隆志

日本植物生理学会年会 2014.3.19

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プリン代謝中間体アラントインはジャスモン酸応答を惹起する

高木絋, 江草真由美, 渡辺俊介, 森泉, 平山隆志, 島田裕士, 上中弘典, 坂本敦

日本植物生理学会年会 2014.3.19

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Expression analysis of tea genes regulated by light using custom microarray

Niwa, Y, Nishimura, H, Hirayama, T, Sawada, Y, Hirai, M, Nagasaki, H, Nakamura, Y, Kobayashi, E, Watanabe, S, Ogawa, T, Nakamura, Y, Kobayashi, H

2014.3.19

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アブシジン酸は表皮細胞の突起形成と微小管の脱重合を引き起こす

高谷彰吾, 橋本隆, 平山隆志, 高橋卓, 本瀬宏康

日本植物生理学会年会 2014.3.18

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植物特異的ミトコンドリアmRNA poly(A)制御機構 Invited

平山隆志

第16回オルガネラワークショップ 2014.3.17

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A novel role of poly(A) specific ribonuclease in plants Invited

Hirayama T

RIKEN Symposium: RNA Sciences in Cell and Developmental Biology III 2013.12.7

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A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis

Hirayama T, Matsuura T, Ohtani, M, Hayashi, S

2013.12.5

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ブラキポディウムのTILLING系統の整備 Invited

最相大輔, 松浦恭和, 松島良, 平山隆志

岡山大学資源植物科学研究所共同利用・共同研究拠点ワークショップ「ブラキポディウムが加速する植物科学」 2013.11.29

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植物ミトコンドリアmRNA poly(A)鎖長調節機構

平山隆志, 大谷美沙都, 林晋平

第3回植物RNA研究ネットワークシンポジウム 2013.11.22

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Plant has a unique regulatory mechanism of mitochondrial mRNA polyA status

Takashi Hirayama

8th International Conference for Plant Mitochondrial Biology 2013.9.14

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Bio- and information resources of Brachypodium toward genome-oriented breeding in Triticeae crops Invited

Saisho, D, Matsuura, T, Matsushima, R, Mochida, K, Shinozaki, K, Hirayama

12th International Wheat Genetics Symposium 2013.9.11

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Novel aspects of poly(A)-dependent mRNA metabolism in plant mitochondrial revealed by analysis of lateral root fasciation mutants of Arabidopsis

Otsuka, K, Yagi, Y, Nakamura, T, Nozaki, M, Sato, Y, Ueda, T, Hirayama, T, Sugiyama, M

RNA Meeting 2013.7.25

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The regulatory mechanism of C/N response by mediation of ABA signaling

Lu, Y, Sato, T, Mori, I, Hirayama, T, Yamaguchi, J

2013.3.22

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Plant specific function of polyA specific ribonuclease (PARN)

Hirayama, T, Matsuura, T, Ushiyama, S, Hayashi, S

2013.3.22

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側根原基形成時の細胞分裂制御におけるミトコンドリアmRNA代謝の役割

大塚蔵嵩, 野崎守, 佐藤康, 蜂谷卓士, 野口航, 上田貴志, 平山隆志, 杉山宗隆

日本植物生理学会年会 2013.3.22

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NIMA related kinases interact with floral regulatory factors, FT, TFL1

Takaya, K, Ikeda, R, Kawamoto, N, Sakai, T, Hirayama, T, Goto, K, Araki, T, Takahashi, T, Motose, H

2013.3.22

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植物ミトコンドリアmRNAのpolyA制御機構 Invited

平山隆志

岡山大学資源植物科学研究所共同利用・共同研究拠点ワークショップ「植物ミトコンドリア研究の新展開」 2013.1.28

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ブラキポディウムTILLINGライン整備について Invited

最相大輔, 加藤美奈子, 松浦恭和, 松島良, 持田恵一, 平山隆志

第一回ブラキポディウムワークショップ 2012.11.12

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Plant specific function of polyA specific ribonuclease (PARN) Invited

Takashi Hirayama

Frontiers in Plant RNA Research 2012 2012.10.17

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シロイヌナズナのPARN/AHG2とAHG2 SUPPRESSOR1 (AGS1)はミトコンドリアmRNAのpolyA鎖長調節に関与する

平山隆志, 松浦恭和, 牛山翔, 林晋平

第14回日本RNA学会年会 2012.7.19

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Role of mitochondria in the restriction of formattive cell division at the initial stage of lateral root primordium development.

Sugiyama, M, Otsuka, K, Konishi, M, Kinoshita, A, Hachiya, T, Noguchi, K, Ueda, T, Hirayama, T

23rd International Conference of Arabidopsis Research 2012.7.6

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AHG2/PARN and AHG2 suppressor 1 (AGS1) regulate the polyA status of mitochondrial mRNA.

Hirayama, T, Matsuura, T, Ushiyama, S, Hayashi, S

23rd International Conference of Arabidopsis Research 2012.7.5

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Pentatricopeptide repeat (PPR)モチーフのRNA認識コード

八木祐介, 林晋平, 小林啓子, 平山隆志, 中村崇裕

日本植物生理学会年会 2012.3.17

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Unique function of Arabidopsis seed predominant PP2Cs, AHG1 and AHG3, in ABA response in seeds and early seedlings

Ushiyama, S, Otagiri, M, Ino, Y, Umezawa, T, Hirayama, T

2012.3.17

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多重ホルモン処理における遺伝子発現解析

岡本真美, 坪井裕理, 郷田秀樹, 嶋田幸久, 平山隆志

第52回日本植物生理学会年会 2011.3.22

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ABA関連PP2C AHG1, AHG3特異的相互作用因子の解析

牛山翔, 小田切正人, 井野洋子, 梅澤泰史, 平山隆志

第52回日本植物生理学会年会 2011.3.21

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植物ホルモンの働きとストレス応答 Invited

平山隆志

第２７回資源植物科学シンポジウム並びに第３回植物ストレス科学研究シンポジウム 2011.3.7

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シロイヌナズナpolyA分解酵素AHG2/PARNの機能と細胞応答統御機構 Invited

平山隆志

シンポジウム, RNAから植物を考える〜植物RNA研究の最先端〜 2010.8.27

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The PP2C-SnRK2 complex: A central regulatory module of protein phosphorylation networks in abscisic acid signaling.

Umezawa, T, Sugiyama, N, Mizoguchi, M, Hayashi, S, Myouga, F, Shinozaki, K.Y, Ishihama, Y, Hirayama, T, Shinozaki, K

21th International Conference of Arabidopsis Research. 2010.6.9

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Analysis of a PARN deficient mutant, ABA hypersensitive germination2-1, of Arabidopsis.

Hirayama, T, Ushiyama, S, Hayashi S

21th International Conference of Arabidopsis Research 2010.6.9

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Analysis of a PARN deficient mutant, ABA hypersensitive germination2-1, of Arabidopsis.

Hirayama, T, Ushiyama, S, Hayashi S

The 19th CDB Meeting: RNA Sciences in Cell and Developmental Biology. 2010.3.11

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Research Projects

ミトコンドリアmRNA動体と細胞機能間相互作用の分子機構解明

Grant number：25K02299 2025.04 - 2028.03

日本学術振興会科学研究費助成事業基盤研究(B)

平山隆志, 間宮章仁

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Grant amount：\18850000 （ Direct expense: \14500000 、 Indirect expense：\4350000 ）

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Development of novel bio-nanodevice for sustainable agriculture

Grant number：21K19117 2021.07 - 2023.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory) Grant-in-Aid for Challenging Research (Exploratory)

平山隆志, 林靖彦, 持田恵一

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Grant amount：\6500000 （ Direct expense: \5000000 、 Indirect expense：\1500000 ）

申請者らは、これまでに、植物を含む生物の経時的な成長状態や生理状態データを用いた非線形の形質予測モデルを構築することにより、環境要因や遺伝要因データから生体の動態を予測することが可能であることを実証した。一方で、予測に必要なデータの取得は、現段階ではコスト、時間、労力の点で効率が低く、より簡便に生体の生理状態に関するデータを取得する技術の開発が、基礎生物学のみならずその社会実装を実現する医学、農学においても不可欠である。本研究は、センサー開発においてコスト、操作、そして倫理的な点においてもハードルが低い植物を用いて、現在注目を集めているカーボンナノチューブ（CNT/SWNT）を利用して生理状態を把握する生体センサーを開発することを目指している。2021年度は、まずその観測システムの構築をおこなった。CNT/SWNTを用いて植物体内の活性酸素の変動を観測した報告を参考に、観測システム構築を行なった。これまでに、ほぼ同様のCNT/SWNTを利用した報告例が２報あるが、互いに異なるデータもあり、その検証や改良点の抽出も含めた検証実験との位置付けである。その結果、いずれの報告とも多々で相違が見受けられたものの、タバコ葉において傷害で誘導された活性酸素を観測することに、独自に構築したシステムを利用して成功した。また、CNT/SWNTをDNAで分散させる場合の条件検討も試みた。さらに、新たな物質のセンサー開発のために、生体分子のアプタマーとして報告があるDNAの情報を探索し、それらを合成しセンサー開発を進めた。この他、異なる薄幕を用いた基盤を利用したセンサーの開発についても検討を始めた。

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Dissection of iron deficiency response involving novel short peptide FEP1.

Grant number：21H02509 2021.04 - 2024.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

平山隆志, 馬建鋒

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Grant amount：\17420000 （ Direct expense: \13400000 、 Indirect expense：\4020000 ）

シロイヌナズナ短鎖ペプチドFEP1がどのように鉄欠乏応答の制御および鉄ホメオスタシスに関わっているかを明らかにするため、独自に作成したfep1欠損株やFEP1誘導発現形質転換株を利用して網羅的遺伝子発現解析を行った。詳細なデータ解析から、FEP1は維管束組織おける鉄欠乏応答で重要な役割を持つという知見を得た。また、FEP1類似遺伝子の植物界における保存性を調査し、維管束系を持つシダ植物にはFEP1類似遺伝子があるが、コケ植物には見当たらず、維管束組織との関わりが示唆された。シダ植物のFEP1様遺伝子はシロイヌナズナのものと同様鉄欠乏により誘導されるが、FEP1の活性に不可欠となるC末端の構造が異なっており機能については不明な点が多いが、ナス科植物にも同様の遺伝子が見つかっており、今後解析が必要である。さらに、FEP1周辺の機能因子の同定を目的に、FEP1を含む鉄欠乏応答遺伝子が恒常的に発現しているミトコンドリアpoly(A)制御関連変異株ahg2-1を背景にして、恒常的鉄欠乏応答形質の抑制変異を2系統獲得しているが、その一つの変異の同定に成功しRNA代謝関連の遺伝子が関与していることを明らかにした。一方、出芽酵母を用いた鉄欠乏応答制御の再構成を試み、植物で見られる応答がほぼ再現された。植物細胞を用いた実験で邪魔となる様々な二次的な影響を排除できる再構成系を用いて、今後構成因子に人為的な変化を導入し、その影響を調査することで精密な制御系の描出が可能と考えている。

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Dissection of iron deficiency response involving novel short peptide FEP1.

Grant number：19K22434 2019.06 - 2021.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

Hirayama Takashi

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Grant amount：\6370000 （ Direct expense: \4900000 、 Indirect expense：\1470000 ）

We identified short peptide genes that are actually translated by comprehensive transcript analysis and ribo-RNAseq analysis from the light-responsive treated plants of the grass model plant, Brachypodium distachyon. These included at least 100 short peptide genes that have not been reported before. To define the sequence set of non-coding RNA genes, we pooled RNA extracted from 21 tissues and analyzed the sequence data of a full-length cDNA library obtained by the cap-trapping method to identify about 10,000 non-redundant lncRNAs candidate sequences. We are attempting to create a discriminant model using some of the protein-coding genes and lncRNA candidate sequences as teacher data.

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Elucidation of the functional molecular mechanisms of seed dormancy mediated by abscisic acid

Grant number：19H02925 2019.04 - 2022.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Nishimura Noriyuki

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Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

DOG1 is one of important components of seed dormancy via abscisic acid (ABA) response. To elucidate the physiological role of DOG1 and its regulatory mechanisms, we identified and characterized novel components that interact with DOG1 and suppress the function of DOG1 in planta. We also performed biochemical analysis of DOG1 and succeeded in identifying new functional amino acid residues that are important for DOG1 function.

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Data driven crop design technology

2016.10 - 2022.03

Japan Science and Technology Agency CREST

平山隆志, 持田恵一, 辻寛之, 梅崎太造

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Authorship：Principal investigator

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Meta heuristics in field environment: improvement of agronomic sustainability and productivity through optimization of soil, weather, and crops

Grant number：15KT0038 2015.07 - 2019.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

Mochida Keiichi, Takahagi Kotaro, Sakurai Tetsuya, Matsuura Takakazu

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Grant amount：\17810000 （ Direct expense: \13700000 、 Indirect expense：\4110000 ）

In this study, we aimed to integrate various physiological data from crops grown under field conditions by transcriptome and hormone analysis throughout their life-course, and to develop a basis for agri-data science to maximize crop productivity and sustainability through computational modeling. For this purpose, we investigated time series transcriptome and hormone datasets from a wild barley and a landrace accession, and revealed physiological changes along with their growth. We also analyzed genome-scale polymorphisms of diverse barley accessions. Moreover, using a legacy dataset of heading date of barely accessions and weather datasets, we developed statistical and/or machine learning-based models that estimate barely heading date.

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Mechanisms of mRNA alternative splicing and ABA-responsive phosphosignaling

Grant number：15H04383 2015.04 - 2018.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

Umezawa Taishi

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Grant amount：\18200000 （ Direct expense: \14000000 、 Indirect expense：\4200000 ）

Abscisic acid (ABA) is a major phytohormone which regulates stress responses in plants. It had been demonstrated that protein phosphorylation is important in ABA signal transduction pathway, and we found several mRNA splicing factors as phosphoproteins in response to ABA. To elucidate splicing events in response to ABA, we performed RNA-seq analysis using Arabidopsis WT and srk2dei mutant, and identified over 170 splice variants were regulated by ABA and SnRK2. We are going to functional analysis of splice variants in ABA signaling, and RNA-seq analysis using Arabidopsis seeds in the same way.

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Comprehensive analysis of the abscisic acid signaling network in higher plant seeds.

Grant number：24370023 2012.04 - 2016.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

Hirayama Takashi, UMEZAWA Taishi, MATUURA Takakazu

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Grant amount：\18850000 （ Direct expense: \14500000 、 Indirect expense：\4350000 ）

In order to understand the protein-phosphorylation net-work responding to a phytohormone, abscisic acid, which is one of the major regulators of germination and abiotic stress response in germination, molecular genetic and phospho-protemic approaches were conducted. We successfully identified the interactors of seed specific ABA-related PP2C, AHG1 and AHG3, and detected phosphoproteins in Arabidopsis seeds and barleys by phosphoproteomic analysis.

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植物のミトコンドリアｍＲＮＡ制御機構の解明

Grant number：23112715 2011.04 - 2013.03

日本学術振興会科学研究費助成事業新学術領域研究(研究領域提案型) 新学術領域研究(研究領域提案型)

平山隆志

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Grant amount：\9360000 （ Direct expense: \7200000 、 Indirect expense：\2160000 ）

高等植物シロイヌナズナのミトコンドリアのmRNA安定制御に関わると考えられるpolyA特異的RNA分解酵素AHG2/PARNとpolyA付加酵素AGS1の機能解明を進めている。平成２４年度は、主にahg2-1変異株のミトコンドリアの性状について解析を行った。そのひとつに、タンパク質の変動を調査した。核ゲノムコードのミトコンドリア局在タンパク質とミトコンドリアゲノムコードのタンパク質についてwestern blottingを行い、蓄積量を調査した。その結果、変異株ではミトコンドリアゲノムコードのタンパク質の増加が認められた。また、Blue-Native-PAGEを用いてミトコンドリア複合体のタンパク質量を調査した。その結果、変異株では、複合体の量が変動していることが確認された。このことから、mRNAの蓄積がタンパク質の増加を引き起こし、それがタンパク質複合体の量比を混乱することにより、呼吸に必要な複合体形成が阻害もしは不安定化を引き起こしていると推察される。また、ミトコンドリアDNA量、RNA量をqPCRを用いて、解析し、変異株では核あたりのミトコンドリア量が増加ていること、更にミトコンドリアDNAあたりのmRNA量が増加していることを明らかにした。また、予備実験としてAHG2, AGS1それぞれと相互作用するタンパク質の同定を試みた。それぞれのGFP結合タンパク質を発現する形質転換体からミトコンドリアを抽出し、供沈殿するタンパク質を質量分析装置を用いて、解析した。この懐石には理化学研究所の支援研究を利用させていただいた。その結果、それぞれ、ミトコンドリアで機能するRNA制御因子との相互作用が認められた。これらのデータを出すとともに、当該年度は論文の執筆を行った。現在、投稿した論文はrevise中で、まもなく投稿する予定である。

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高等植物のRNA制御と環境応答機構に関する分子生物学的解析

Grant number：21112521 2009 - 2010

日本学術振興会科学研究費助成事業新学術領域研究(研究領域提案型) 新学術領域研究(研究領域提案型)

平山隆志

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Grant amount：\9100000 （ Direct expense: \7000000 、 Indirect expense：\2100000 ）

昨年度までに、polyA特異的RNA分解酵素のAHG2の機能解析と、ahg2-1変異株の抑制変異遺伝子として分離されたpolyA付加酵素をコードするAGSI遺伝子の同定を進めてきた。本年度は、これら因子の標的分子の探索を中心に研究を進めた。ahg2-1変異を対象とした網羅的代謝物解析から、ミトコンドリア内転写物の蓄積が認められた。ミトコンドリア機能異常変異atphb3との比較解析から、ahg2-1変異はミトコンドリアに何らかの異常を持つことが示唆されたので、ミトコンドリア転写物いくつかのpolyA状態をPAT法により調査した。その結果、調べた転写物のほとんどでpolyA付加が認められた。一方野生株、ags1,atphb3ではpolyA付加は認められなかった。さらにinverse PCR法でnad7転写物のpolyA鎖長の状態を詳細に調べたところ、野生株ではpolyA付加が認められなかったが、ahg2-1では6～17bのpolyAを持つ転写物が確認された。また、ミトコンドリア転写物のいくつかについてNorthern法により蓄積量を調査したところ、調べたほとんどの転写物のahg2-1での蓄積が認められた。以上の結果から、AHG2およびAGS1の標的はミトコンドリア転写物と考えられる。これまで植物はもちろん他の真確生物に於いてもミトコンドリアmRNAのpolyA付加および除去に関わる因子双方が同定されている例はほとんどなく、その制御が不明であったため、この研究成果は意義が大きいと考える。
しかしながら、これまでのところ、AHG2とAGS1はともにミトコンドリア局在を示す実験結果はない。これらのN末には、ミトコンドリア移行配列様構造が存在するので、これらを除いた改変遺伝子がahg2-1変異を相補できるかを検討し、ミトコンドリア局在と機能の関連を調査している。

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Analysis of ABA response network using novel ABA response mutants of Arabidopsis

Grant number：20570050 2008 - 2010

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

HIRAYAMA Takashi, UMEZAWA Taishi

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Grant amount：\4940000 （ Direct expense: \3800000 、 Indirect expense：\1140000 ）

Molecular genetic and molecular biological analyses of several novel Arabidopsis mutants that impair the abscisic acid (ABA)-response allowed us to describe the ABA signal transduction pathway from perception to nuclear events and find the novel relation between ABA response and mitochondrial function. These findings offer a new avenue for understanding the cellular response mechanisms to ABA.

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Molecular genetics of abscisic acid signaling pathway

2008 - 2010

Grant-in-Aid for Scientific Research

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Grant type：Competitive

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Analysis of GEK1 function that is required for ethanol tolerance in higher plants

Grant number：15570045 2003 - 2004

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

HIRAYAMA Takashi

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Grant amount：\3700000 （ Direct expense: \3700000 ）

1.Phenotypic analysis using gek1 mutants.
To investigate the effect of gek1 mutation on the metabolic pathway, we tried to detect the change in metabolites by muti-nuclear NMR measurement combined with ^<13>C, ^<15>N stable isotope labelling. The results showed that gek1 activated the amino acid metabolic pathway in the presence of ethanol more strongly than the wild type. Microarray analysis revealed that several stress inducible genes were upregulated more rapidly by ethanol treatment in the gekl mutant.
2.Screening for gek1 suppressor mutants.
Putative gek1 suppressor mutants, totally 34 lines, were isolated. We analyzed most of them and found they were adh mutants. We are going to analyze rest of them.
3.Identification GEK1-interacting proteins.
Three putative GEK1-interacting proteins were identified by yeast two-hybrid analysis. Two of them are Zn-finger proteins (#20,#24). The rest one is NFU2(#76) that is presumed to be involved in the formation of Fe-S cluster in chloroplasts. The subcellular localization and the analyses of disruption mutants (#24,#76) did not offer any clear link between these proteins and GEK1 so far.
4.Recombinant GEK1 protein.
A recombinant GEK1 protein expressed in E.coli was obtained. We investigated its activity to breakdown acetaldehyde in vitro. We tried several conditions, but failed to detect such activity. We obtained the recombinant protein of the Pyrococcus GEK1 homologus gene that is presumed to be more stable. We tried to detect the same activity but failed again. Therefore, we concluded that GEK1 does not have such activity. We also tried to see any activity to change the metabolite profiling. The extract from ^<13>C,^<15>N stable isotope labeled plants were reacted with the recombinant protein and measured by NMR. However, we could not detect any changes.

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Isolation and analysis of mutants with altered ABA recognition specificity using an ABA antagonist.

Grant number：12640642 2000 - 2001

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

HIRAYAMA Takashi

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Grant amount：\4100000 （ Direct expense: \4100000 ）

In order to identify the ABA receptors, we employed a novel screening using an ABA binding inhibitor to isolate mutants that have altered binding specificity of ABA receptors. We used a ABA analogue, PBI-51, as a ABA binding inhibitor since this chemical has been reported to work as ABA binding inhibitor in Brassica napus. We tried to isolate mutants with two type of screening strategies. One is to search for mutants that seem to recognize PB151 as an ABA binding inhibitor. We isolated several candidates from 500,000 M2 seeds from EMS treated plants. However, these candidates turned out to be ABA insensitive mutants. The other strategy was to isolate mutants that seems recognize PB151 as ABA. In this screen, we isolated 8 independent lines from 400,000 M2 seeds from EMS treated plants. Those candidates show ABA-hypersensitivity, although these are not allelic to known ABA-hypersensitive mutants, eral or ein2.To increase the efficiency to isolate ABA receptor mutants, we thought out the strategy to use aba2 mutants, and isolated several dozens of candidates. ldentification of those mutated genes will surely contribute in our understanding on ABA siganling pathway. In the course to isolate ABA related mutants, we isolated a novel ethanol hypersensitiv mutant, gekol, from Arabidopsis. We identified the GEKO I gene by mapping and found it encoded a novel protein that conserved among plants and archea bacteria. GEKO I may play a pivotal role for plant sp ecific functions.

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Genetic and molecular analysis on an ethylene signaling pathway in Arabidopsis

1996.09 - 1998.08

日本医療研究開発機構 Human Frontier Science Program Biology

Joseph R. Ecker

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Authorship：Principal investigator

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酵母の高浸透圧耐性突然変異株の分離と浸透圧ストレス応答性遺伝子の発現について

Grant number：05740458 1993

日本学術振興会科学研究費助成事業奨励研究(A) 奨励研究(A)

平山隆志

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Grant amount：\1000000 （ Direct expense: \1000000 ）

1、酵母の高浸透圧応答性遺伝子の単離及び解析
出芽酵母よりディファレンシャルスクリーニング法を用いて高浸透圧応答性遺伝子のcDNAクローン7つ(HOR1-HOR7)を単離した。これらのクローン構造解析を行い、HOR1,HOR3,HOR4,HOR5,HOR6がそれぞれglycerol-3-phosphate dehidrogenase,glucokinase,hexose,transporter,HSP12,P-type A TPaseをコードしていることが明らかにした。HOR2,HOR7は、今まで報告されていない遺伝子であった。さらにHOR2遺伝子と構造の類似してはいるが恒常的に発現している遺伝子、RHR2が酵母にはあることがわかった。このことから、HOR2及びRHR2の産物は酵母の生育にとって重要な働きを担っていると考えられたが、これらの遺伝子の破壊株は特徴的な表現型を示さなかった。
様々な変異株のおけるこれらの7つのHOR遺伝子の発現様式を調べることで、情報伝達系路についてある程度示唆を得た。まず、高浸透圧応答には、いくつかの情報伝達経路があると予想された。また、いくつかの遺伝子はcAMPによっても制御されている可能性も示唆された。
2、高浸透圧応答に関与する情報伝達突然変異体の単離
単離した高浸透圧応答性遺伝子HOR2の発現調節領域を大腸菌由来のLacZ遺伝子に結合しレポーター遺伝子を構築した。このレポータ遺伝子は高浸透圧に応答した。このレポーター遺伝子を導入した酵母菌を用いて高浸透圧応答に異常の見られる突然変異株の分離を試みている。

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植物の細胞周期を制御するCDC2遺伝子ファミリーの研究

Grant number：03780230 1991

日本学術振興会科学研究費助成事業奨励研究(A) 奨励研究(A)

平山隆志

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Grant amount：\700000 （ Direct expense: \700000 ）

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