Faculty Profiles - NAKA Shuuhei

Papers - NAKA Shuuhei

Division display 21 - 40 of about 81 ／ All the affair displays >>

口腔内のcnm遺伝子陽性S.mutansとIgA腎症患者の糖鎖異常IgA沈着の関連性

三崎太郎, 仲周平, 鈴木仁, 李明峰, 青木良輔, 長澤康行, 松岡大貴, 伊藤誓悟, 野村良太, 仲野道代, 鈴木祐介, 仲野和彦

日本腎臓学会誌 65 ( 3 ) 271 - 271 2023.5

腎炎のあたらしい"モデル動物"の開発ラットおよびマウスを用いた口腔細菌のIgA腎症発症への影響の検討

野村良太, 長澤康行, 三崎太郎, 仲周平, 仲野道代, 仲野和彦

日本腎臓学会誌 65 ( 3 ) 210 - 210 2023.5

Cnm陽性Streptococcus mutansの分子生物学的解析(Molecular biological analysis of Cnm positive Streptococcus mutans)

Matsuoka Daiki, Naka Shuhei, Goto Kana, Nomura Ryota, Nakano Kazuhiko, Matsumoto-Nakano Michiyo

小児歯科学雑誌 61 ( 大会抄録号 ) 274 - 274 2023.4

cnm-positive Streptococcus mutans is associated with galactose-deficient IgA in patients with IgA nephropathy. International journal

Taro Misaki, Shuhei Naka, Hitoshi Suzuki, Mingfeng Lee, Ryosuke Aoki, Yasuyuki Nagasawa, Daiki Matsuoka, Seigo Ito, Ryota Nomura, Michiyo Matsumoto-Nakano, Yusuke Suzuki, Kazuhiko Nakano

PloS one 18 ( 3 ) e0282367 2023

Language：English Publishing type：Research paper (scientific journal)

The presence of Streptococcus mutans expressing Cnm protein encoded by cnm (cnm-positive S. mutans) in the oral cavity is associated with immunoglobulin A (IgA) nephropathy (IgAN). However, the precise mechanism by which cnm-positive S. mutans is involved in the pathogenesis of IgAN remains unclear. The present study evaluated glomerular galactose-deficient IgA1 (Gd-IgA1) to clarify the association between the presence of cnm-positive S. mutans and glomerular Gd-IgA1 in patients with IgAN. The presence of S. mutans and cnm-positive S. mutans was evaluated by polymerase chain reaction in saliva specimens from 74 patients with IgAN or IgA vasculitis. Immunofluorescent staining of IgA and Gd-IgA1 using KM55 antibody in clinical glomerular tissues was then performed. There was no significant association between the glomerular staining intensity of IgA and the positive rate of S. mutans. However, there was a significant association between the glomerular staining intensity of IgA and the positive rate of cnm-positive S. mutans (P < 0.05). There was also a significant association between the glomerular staining intensity of Gd-IgA1 (KM55) and the positive rate of cnm-positive S. mutans (P < 0.05). The glomerular staining intensity of Gd-IgA1 (KM55) was not associated with the positive rate of S. mutans. These results suggest that cnm-positive S. mutans in the oral cavity is associated with the pathogenesis of Gd-IgA1 in patients with IgAN.

DOI： 10.1371/journal.pone.0282367

PubMed

researchmap

Distribution of periodontopathic bacterial species between saliva and tonsils.

Ryota Nomura, Yasuyuki Nagasawa, Taro Misaki, Seigo Ito, Shuhei Naka, Mieko Okunaka, Maiko Watanabe, Kenzo Tsuzuki, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Odontology 111 ( 3 ) 719 - 727 2022.12

Language：English Publishing type：Research paper (scientific journal)

Periodontopathic bacteria cause an inflammatory disease localized in the periodontal tissue and are associated with various conditions in other body parts. The distribution of periodontopathic bacterial species in the tonsils is unknown, even though the tonsils are located close to the oral cavity, and inflammation of the tonsils causes various systemic diseases. We detected the major periodontopathic bacterial species residing in saliva and tonsil specimens from 25 subjects undergoing tonsillectomy. Nine of the ten major periodontopathic bacterial species were detected by polymerase chain reaction of tonsil specimens, among which Campylobacter rectus was the most common (80.0%), followed by Porphyromonas gingivalis (36.0%). The other seven types of periodontopathic bacterial species were distributed with 0% to 25.0% abundance in the tonsil specimens. C. rectus had a high detection rate in tonsil specimens (> 75.0%), regardless of whether it was detected in the corresponding saliva specimens. However, the detection rate for P. gingivalis in tonsil specimens was significantly higher in subjects with P. gingivalis-positive saliva (77.8%) than in those with P. gingivalis-negative saliva (6.3%; P < 0.001). Furthermore, 75.0% of P. gingivalis in tonsil specimens did not have the known fimA gene that encodes the 41-kDa filamentous appendage protein FimA, which is expressed on the cell surface of the bacteria. Our results suggest that certain periodontopathic bacterial species are detected in the tonsils either independently of or depending on their distribution in the oral cavity and may be involved in tonsil-related diseases.

DOI： 10.1007/s10266-022-00776-8

PubMed

researchmap

Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability Reviewed

Shuhei Naka, Daiki Matsuoka, Kana Goto, Taro Misaki, Yasuyuki Nagasawa, Seigo Ito, Ryota Nomura, Kazuhiko Nakano, Michiyo Matsumoto-Nakano

Frontiers in Cellular and Infection Microbiology 12 2022.9

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers Media SA

Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries. The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown. In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm. Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure. Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated. The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides. Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains. Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.

DOI： 10.3389/fcimb.2022.994014

researchmap

Simultaneous Presence of Campylobacter rectus and Cnm-Positive Streptococcus mutans in the Oral Cavity Is Associated with Renal Dysfunction in IgA Nephropathy Patients: 5-Year Follow-Up Analysis. International journal

Taro Misaki, Shuhei Naka, Yasuyuki Nagasawa, Daiki Matsuoka, Seigo Ito, Ryota Nomura, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Nephron 147 ( 3-4 ) 1 - 10 2022.8

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: The simultaneous presence of Streptococcus mutans expressing the Cnm protein encoded by cnm (i.e., cnm-positive S. mutans) and Campylobacter rectus in the oral cavity has been associated with proteinuria in patients with IgA nephropathy (IgAN). OBJECTIVES: The present study evaluated the relationship between renal function and oral bacteria in patients with IgAN over 5 years of follow-up. METHODS: The presence of C. rectus and cnm-positive S. mutans in saliva samples of 117 patients with IgAN was initially evaluated by polymerase chain reaction. Patients were then divided into four groups according to the results of C. rectus and cnm-positive S. mutans detection: group A: C. rectus (-), cnm-positive S. mutans (-); group B: C. rectus (+), cnm-positive S. mutans (-); group C: C. rectus (-), cnm-positive S. mutans (+); and group D: C. rectus (+), cnm-positive S. mutans (+). Clinical characteristics were prospectively followed for 5 years. RESULTS: Serum creatinine levels were significantly higher in group D than in group A over 5 years of follow-up. Additionally, the proportion of patients with an estimated glomerular filtration rate <45 mL/min increased over time; it was significantly greater in group D than in group A over 5 years of follow-up. CONCLUSION: These results suggest that the simultaneous presence of C. rectus and cnm-positive S. mutans in the oral cavity is associated with renal dysfunction in IgAN patients.

DOI： 10.1159/000525511

PubMed

researchmap

Porphyromonas gingivalis経鼻投与によるIgA腎症モデルマウスの作製

長澤康行, 野村良太, 三崎太郎, 仲周平, 伊藤誓悟, 和唐薫子, 楠博, 山崎博充, 新村健, 仲野道代, 仲野和彦

日本腎臓学会誌 64 ( 3 ) 243 - 243 2022.5

cnm遺伝子陽性S.mutansとC.rectusの口腔内の存在は5年後のIgA腎症患者の腎機能増悪に関連する

三崎太郎, 塩崎友里子, 伊藤大介, 清水吉貴, 鈴木由美子, 仲周平, 野村良太, 長澤康行, 伊藤誓悟, 仲野道代, 仲野和彦

日本腎臓学会誌 64 ( 3 ) 243 - 243 2022.5

Title IgA Nephropathy and Oral Bacterial Species Related to Dental Caries and Periodontitis. Reviewed International journal

Yasuyuki Nagasawa, Taro Misaki, Seigo Ito, Shuhei Naka, Kaoruko Wato, Ryota Nomura, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

International journal of molecular sciences 23 ( 2 ) 2022.1

Language：English Publishing type：Research paper (scientific journal)

A relationship between IgA nephropathy (IgAN) and bacterial infection has been suspected. As IgAN is a chronic disease, bacteria that could cause chronic infection in oral areas might be pathogenetic bacteria candidates. Oral bacterial species related to dental caries and periodontitis should be candidates because these bacteria are well known to be pathogenic in chronic dental disease. Recently, several reports have indicated that collagen-binding protein (cnm)-(+) Streptococcs mutans is relate to the incidence of IgAN and the progression of IgAN. Among periodontal bacteria, Treponema denticola, Porphyromonas gingivalis and Campylobacte rectus were found to be related to the incidence of IgAN. These bacteria can cause IgAN-like histological findings in animal models. While the connection between oral bacterial infection, such as infection with S. mutans and periodontal bacteria, and the incidence of IgAN remains unclear, these bacterial infections might cause aberrantly glycosylated IgA1 in nasopharynx-associated lymphoid tissue, which has been reported to cause IgA deposition in mesangial areas in glomeruli, probably through the alteration of microRNAs related to the expression of glycosylation enzymes. The roles of other factors related to the incidence and progression of IgA, such as genes and cigarette smoking, can also be explained from the perspective of the relationship between these factors and oral bacteria. This review summarizes the relationship between IgAN and oral bacteria, such as cnm-(+) S. mutans and periodontal bacteria.

DOI： 10.3390/ijms23020725

PubMed

researchmap

Relationship between IgA Nephropathy and Porphyromonas gingivalis; Red Complex of Periodontopathic Bacterial Species. Reviewed International journal

Yasuyuki Nagasawa, Ryota Nomura, Taro Misaki, Seigo Ito, Shuhei Naka, Kaoruko Wato, Mieko Okunaka, Maiko Watabe, Katsuya Fushimi, Kenzo Tsuzuki, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

International journal of molecular sciences 22 ( 23 ) 2021.12

Language：English Publishing type：Research paper (scientific journal)

IgA nephropathy (IgAN) has been considered to have a relationship with infection in the tonsil, because IgAN patients often manifest macro hematuria just after tonsillitis. In terms of oral-area infection, the red complex of periodontal bacteria (Porphyromonas gingivalis (P. gingivalis), Treponema denticol (T. denticola) and Tannerella forsythia (T. forsythia)) is important, but the relationship between these bacteria and IgAN remains unknown. In this study, the prevalence of the red complex of periodontal bacteria in tonsil was compared between IgAN and tonsillitis patients. The pathogenicity of IgAN induced by P. gingivalis was confirmed by the mice model treated with this bacterium. The prevalence of P. gingivalis and T. forsythia in IgAN patients was significantly higher than that in tonsillitis patients (p < 0.001 and p < 0.05, respectively). A total of 92% of tonsillitis patients were free from red complex bacteria, while only 48% of IgAN patients had any of these bacteria. Nasal administration of P. gingivalis in mice caused mesangial proliferation (p < 0.05 at days 28a nd 42; p < 0.01 at days 14 and 56) and IgA deposition (p < 0.001 at day 42 and 56 after administration). Scanning-electron-microscopic observation revealed that a high-density Electron-Dense Deposit was widely distributed in the mesangial region in the mice kidneys treated with P. gingivalis. These findings suggest that P. gingivalis is involved in the pathogenesis of IgAN.

DOI： 10.3390/ijms222313022

PubMed

researchmap

扁桃と口腔内の歯周病菌の一致率の検討

長澤康行, 野村良太, 三崎太郎, 仲周平, 伊藤誓悟, 和唐薫子, 木田有利, 名波正義, 倉賀野隆裕, 仲野道代, 仲野和彦

日本腎臓学会誌 63 ( 4 ) 494 - 494 2021.6

Inhibitory effects of flavedo, albedo, fruits, and leaves of Citrus unshiu extracts on Streptococcus mutans. Reviewed International journal

Ryota Nomura, Jumpei Ohata, Masatoshi Otsugu, Rena Okawa, Shuhei Naka, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Archives of oral biology 124 105056 - 105056 2021.4

Language：English Publishing type：Research paper (scientific journal)

OBJECTVES: Citrus unshiu has been shown to exhibit antimicrobial effects against citrus diseases. In the present study, C. unshiu was divided into flavedo, albedo, fruits, and leaves; the inhibitory effects of these extracts on Streptococcus mutans, a major pathogen of dental caries, were investigated. DESIGN: C. unshiu specimens were separated into flavedo, albedo, fruits, and leaves. First, pH values and polyphenol amounts in Citrus extracts were measured. In addition, Citrus extract was added to the bacterial suspensions of S. mutans MT8148, and inhibitory effects of C. unshiu extracts on MT8148 for antimicrobial activity, bacterial growth, and biofilm formation were analyzed. These assays were also performed using C. sinensis extracts. RESULTS: Among these extracts, albedo exhibited a pH value closest to neutral, while the fruits exhibited the most acidic pH value; the pH values significantly differed between these extracts (P < 0.05). In addition, the amounts of polyphenols were significantly higher in albedo than in other extracts (P < 0.001). All extracts showed inhibitory effects on MT8148 for antimicrobial activity, bacterial growth and biofilm formation. These inhibitory effects were significantly stronger in flavedo, albedo, and fruits, compared with leaves (P < 0.05). Furthermore, extracts of Citrus sinensis also showed inhibitory effects on S. mutans, although these effects were weaker than the effects of C. unshiu. CONCLUSION: These results suggest that extracts from C. unshiu fruits exhibit inhibitory effects on S. mutans, among which albedo may be especially useful for dental caries prevention due to its neutral pH and abundant polyphenols, in addition to its inhibitory effects.

DOI： 10.1016/j.archoralbio.2021.105056

PubMed

researchmap

Streptococcus mutans induces IgA nephropathy-like glomerulonephritis in rats with severe dental caries. Reviewed International journal

Shuhei Naka, Kaoruko Wato, Taro Misaki, Seigo Ito, Daiki Matsuoka, Yasuyuki Nagasawa, Ryota Nomura, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Scientific reports 11 ( 1 ) 5784 - 5784 2021.3

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

The mechanisms underlying immunoglobulin A nephropathy (IgAN), the most common chronic form of primary glomerulonephritis, remain poorly understood. Streptococcus mutans, a Gram-positive facultatively anaerobic oral bacterium, is a common cause of dental caries. In previous studies, S. mutans isolates that express Cnm protein on their cell surface were frequently detected in IgAN patients. In the present study, inoculation of Cnm-positive S. mutans in the oral cavities of 2-week-old specific-pathogen free Sprague-Dawley rats fed a high-sucrose diet for 32 weeks produced severe dental caries in all rats. Immunohistochemical analyses of the kidneys using IgA- and complement C3-specific antibodies revealed positive staining in the mesangial region. Scanning electron microscopy revealed a wide distribution of electron dense deposits in the mesangial region and periodic acid-Schiff staining demonstrated prominent proliferation of mesangial cells and mesangial matrix. These results suggest that IgAN-like glomerulonephritis was induced in rats with severe dental caries by Cnm-positive S. mutans.

DOI： 10.1038/s41598-021-85196-4

PubMed

researchmap

Non-alcoholic steatohepatitis caused by oral bacteria Invited Reviewed

Shuhei Naka, Michiyo Matsumoto-Nakano

Pediatric Dental Journal 31 11 - 16 2021.2

Intravenous administration of Streptococcus mutans induces IgA nephropathy-like lesions. Reviewed

Shuhei Naka, Kaoruko Wato, Taro Misaki, Seigo Ito, Yasuyuki Nagasawa, Ryota Nomura, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Clinical and experimental nephrology 24 ( 12 ) 1122 - 1131 2020.12

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: IgA nephropathy (IgAN) is one of the most frequently occurring types of chronic glomerulonephritis. Previous analyses have revealed that a major pathogen of dental caries, Streptococcus mutans [which expresses collagen-binding protein (Cnm) on its surface], is involved in the pathogenesis of IgAN. METHODS: Cnm-positive S. mutans isolated from a patient with IgAN was intravenously administered to specific pathogen-free Sprague-Dawley rats to evaluate their kidney conditions. RESULTS: The urinary protein level of the S. mutans group reached a plateau at 30 days, with increased numbers of mesangial cells and an increased mesangial matrix. The numbers of rats with IgA-positive and/or C3-positive glomeruli were significantly greater in the S. mutans group than in the control group at 45 days (P < 0.05). Electron microscopy analyses revealed electron-dense depositions in the mesangial area among rats in the S. mutans group. There were significantly more CD68-positive cells (macrophages) in the glomeruli of the S. mutans group than in the glomeruli of the control group during the late phase (P < 0.05), similar to the findings in patients with IgAN. CONCLUSION: Our results suggested that intravenous administration of Cnm-positive S. mutans caused transient induction of IgAN-like lesions in rats.

DOI： 10.1007/s10157-020-01961-1

PubMed

researchmap

Potential involvement of Streptococcus mutans possessing collagen binding protein Cnm in infective endocarditis. Reviewed International journal

Ryota Nomura, Masatoshi Otsugu, Masakazu Hamada, Saaya Matayoshi, Noboru Teramoto, Naoki Iwashita, Shuhei Naka, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Scientific reports 10 ( 1 ) 19118 - 19118 2020.11

Language：English Publishing type：Research paper (scientific journal)

Streptococcus mutans, a significant contributor to dental caries, is occasionally isolated from the blood of patients with infective endocarditis. We previously showed that S. mutans strains expressing collagen-binding protein (Cnm) are present in the oral cavity of approximately 10-20% of humans and that they can effectively invade human umbilical vein endothelial cells (HUVECs). Here, we investigated the potential molecular mechanisms of HUVEC invasion by Cnm-positive S. mutans. The ability of Cnm-positive S. mutans to invade HUVECs was significantly increased by the presence of serum, purified type IV collagen, and fibrinogen (p < 0.001). Microarray analyses of HUVECs infected by Cnm-positive or -negative S. mutans strains identified several transcripts that were differentially upregulated during invasion, including those encoding the small G protein regulatory proteins ARHGEF38 and ARHGAP9. Upregulation of these proteins occurred during invasion only in the presence of serum. Knockdown of ARHGEF38 strongly reduced HUVEC invasion by Cnm-positive S. mutans. In a rat model of infective endocarditis, cardiac endothelial cell damage was more prominent following infection with a Cnm-positive strain compared with a Cnm-negative strain. These results suggest that the type IV collagen-Cnm-ARHGEF38 pathway may play a crucial role in the pathogenesis of infective endocarditis.

DOI： 10.1038/s41598-020-75933-6

PubMed

researchmap

Identification and functional analysis of glutamine transporter in Streptococcus mutans. Reviewed International journal

Yuko Morikawa, Setsuyo Morimoto, Eri Yoshida, Shuhei Naka, Hiroaki Inaba, Michiyo Matsumoto-Nakano

Journal of oral microbiology 12 ( 1 ) 1797320 - 1797320 2020.8

Language：English Publishing type：Research paper (scientific journal)

Background: Streptococcus mutans, a biofilm-forming bacterium, possesses several transporters that function as import/export molecules. Among them, the PII protein family is composed of members that regulate glutamine synthesis in bacterial species. Objective: In this study, we characterized the function of the glutamine transporter in S. mutans MT8148. Methods: The SMU.732 gene, corresponding to glnP in S. mutans, is homologous to the glutamine transporter gene in Bacillus subtilis. We constructed a glnP-inactivated mutant strain (GEMR) and a complement strain (comp-GEMR) and evaluated their biological functions. Results: Growth of GEMR was similar in the presence and absence of glutamine, whereas the growth rates of MT8148 and comp-GEMR were significantly lower in the presence of glutamine as compared to its absence. Furthermore, biofilms formed by MT8148 and comp-GEMR were significantly thicker than that formed by GEMR, while the GEMR strain showed a significantly lower survival rate in an acidic environment than the other strains. Addition of n-phenyl-2-naphthylamine, used to label of the membrane, led to increased fluorescence intensity of MT8148 and GEMR, albeit that was significantly lower in the latter. Conclusions: These results suggest that glnP is associated with glutamine transport in S. mutans, especially the import of glutamine involved in biofilm formation.

DOI： 10.1080/20002297.2020.1797320

PubMed

researchmap

Specific strains of Streptococcus mutans, a pathogen of dental caries, in the tonsils, are associated with IgA nephropathy. Reviewed International journal

Seigo Ito, Taro Misaki, Shuhei Naka, Kaoruko Wato, Yasuyuki Nagasawa, Ryota Nomura, Masatoshi Otsugu, Michiyo Matsumoto-Nakano, Kazuhiko Nakano, Hiroo Kumagai, Naoki Oshima

Scientific reports 9 ( 1 ) 20130 - 20130 2019.12

Language：English Publishing type：Research paper (scientific journal)

Streptococcus mutans is known to be a major causative agent of dental caries, and strains expressing the cell surface collagen-binding Cnm protein contribute to the development of several systemic diseases. A relationship between tonsillar immunity and glomerulonephritis has been recognized in IgA nephropathy (IgAN), and specific pathogens may have effects on tonsillar immunity (mucosal immunity). Here, we present findings showing a relationship between the presence of Cnm-positive S. mutans strains in the tonsils of IgAN patients and IgAN condition/pathogenesis. Analyses of tonsillar specimens obtained from patients with IgAN (n = 61) and chronic tonsillitis (controls; n = 40) showed that the Cnm protein-positive rate was significantly higher in IgAN patients. Among IgAN patients, the tonsillar Cnm-positive group (n = 15) had a significantly higher proportion of patients with high urinary protein (>1.5 g/gCr) and lower serum albumin level than the Cnm-negative group (n = 46). Additionally, Cnm protein and CD68, a common human macrophage marker, were shown to be merged in the tonsils of IgAN patients. These findings suggest that Cnm-positive S. mutans strains in the tonsils may be associated with severe IgAN.

DOI： 10.1038/s41598-019-56679-2

PubMed

researchmap

Case of autoimmune neutropenia with severe marginal periodontitis Reviewed

Setsuyo Morimoto, Keiko Hirano, Keiko Tabata, Haruka Asaumi, Yuko Morikawa, Yuki Matsumi, Shuhei Naka, Michiyo Matsumoto-Nakano

PEDIATRIC DENTAL JOURNAL 29 ( 3 ) 138 - 145 2019.12

　 PREV - NEXT 　

< NAKA Shuuhei >

Papers - NAKA Shuuhei