Faculty Profiles - NAKA Shuuhei

Papers - NAKA Shuuhei

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Relationship Between the Presence of Red Complex Species and the Distribution of Other Oral Bacteria, Including Major Periodontal Pathogens in Older Japanese Individuals

Mariko Kametani, Yasuyuki Nagasawa, Momoko Usuda, Ami Kaneki, Masashi Ogawa, Kensaku Shojima, Hiromitsu Yamazaki, Kana Tokumoto, Daiki Matsuoka, Kana Suehara, Yuto Suehiro, Tatsuya Akitomo, Chieko Mitsuhata, Taro Misaki, Seigo Ito, Shuhei Naka, Michiyo Matsumoto-Nakano, Kazuhiko Nakano, Hiromitsu Kishimoto, Ken Shinmura, Ryota Nomura

International Journal of Molecular Sciences 25 ( 22 ) 12243 - 12243 2024.11

Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Red complex bacteria (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) have high virulence in periodontal disease. In the present study, we aimed to elucidate the detailed symbiotic relationships between the red complex and other oral bacteria in older Japanese individuals. Polymerase chain reaction was performed using dental plaque from 116 subjects and specific primers for ten periodontal pathogens. The detection rate of Prevotella intermedia and Capnocytophaga sputigena was significantly higher in P. gingivalis-positive subjects than in P. gingivalis-negative subjects (p < 0.05). The detection rate of Campylobacter rectus, Prevotella nigrescens, Capnocytophaga ochracea, and Eikenella corrodens was significantly higher in T. forsythia-positive subjects than in T. forsythia-negative subjects (p < 0.01). In a comprehensive analysis of oral microbiomes, three red complex species-positive subjects had significantly higher α-diversity than only P. gingivalis-positive subjects (p < 0.05) and had significantly lower β-diversity than only T. forsythia-positive subjects (p < 0.01). In the taxonomy analysis, Porphyromonas was significantly higher in three red complex species-positive subjects than in only P. gingivalis-positive and only T. forsythia-positive subjects (p < 0.01). These results suggest that each red complex species forms a unique oral microbiome and individuals positive for all red complex bacteria may harbor oral bacteria that confer a significant advantage in developing periodontal disease.

DOI： 10.3390/ijms252212243

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Contribution of collagen-binding protein Cnm of Streptococcus mutans to induced IgA nephropathy-like nephritis in rats. International journal

Shuhei Naka, Daiki Matsuoka, Taro Misaki, Yasuyuki Nagasawa, Seigo Ito, Ryota Nomura, Kazuhiko Nakano, Michiyo Matsumoto-Nakano

Communications biology 7 ( 1 ) 1141 - 1141 2024.9

Language：English Publishing type：Research paper (scientific journal)

IgA nephropathy (IgAN), the most common primary glomerulonephritis, is considered an intractable disease with unknown pathogenic factors. In our previous study, Streptococcus mutans, the major causative bacteria of dental caries, which expresses Cnm, was related to the induction of IgAN-like nephritis. In the present study, the Cnm-positive S. mutans parental strain, a Cnm-defective isogenic mutant strain, its complementation strain, and recombinant Cnm (rCnm) protein were administered intravenously to Sprague Dawley rats, and the condition of their kidneys was evaluated focusing on the pathogenicity of Cnm. Rats treated with parental and complement bacterial strains and rCnm protein developed IgAN-like nephritis with mesangial proliferation and IgA and C3 mesangial deposition. Scanning immunoelectron microscopy revealed that rCnm was present in the electron-dense deposition area of the mesangial region in the rCnm protein group. These results demonstrated that the Cnm protein itself is an important factor in the induction of IgAN in rats.

DOI： 10.1038/s42003-024-06826-x

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High-performance silica-containing professional mechanical tooth-cleaning paste that effectively adsorbs pigments

Tatsuya Akitomo, Satoru Kusaka, Mariko Kametani, Chieko Mitsuhata, Shuhei Naka, Michiyo Matsumoto-Nakano, Kazuhiko Nakano, Ryota Nomura

Pediatric Dental Journal 34 ( 2 ) 62 - 69 2024.8

IgA腎症患者と習慣性扁桃炎患者の摘出扁桃における歯周病菌の検出率の比較

長澤康行, 野村良太, 三崎太郎, 伊藤誓悟, 山崎博充, 庄嶋健作, 松岡大貴, 仲周平, 仲野道代, 新村健, 仲野和彦

日本腎臓学会誌 66 ( 4 ) 591 - 591 2024.6

う蝕原性細菌cnm陽性Streptococcus mutansは扁桃を介してIgA腎症を増悪させる

伊藤誓悟, 三崎太郎, 長澤康行, 松岡大貴, 仲周平, 野村良太, 仲野道代, 仲野和彦

日本腎臓学会誌 66 ( 4 ) 637 - 637 2024.6

IgA腎症患者と習慣性扁桃炎患者の摘出扁桃における歯周病菌の検出率の比較

長澤康行, 野村良太, 三崎太郎, 伊藤誓悟, 山崎博充, 庄嶋健作, 松岡大貴, 仲周平, 仲野道代, 新村健, 仲野和彦

日本腎臓学会誌 66 ( 4 ) 591 - 591 2024.6

非アルコール性脂肪肝炎患者由来Streptococcus mutansの各臓器における分布とその特性に関する検討

田畑佳子, 仲周平, 中野聡大, 仲野道代

小児歯科学雑誌 62 ( 大会抄録号 ) 121 - 121 2024.4

中野聡大, 田畑佳子, 仲周平, 仲野道代

小児歯科学雑誌 62 ( 大会抄録号 ) 151 - 151 2024.4

コラーゲン結合タンパクCnmとヒト免疫グロブリンの結合に関する分子生物学的検討

末原佳奈, 松岡大貴, 仲周平, 野村良太, 仲野和彦, 仲野道代, 三崎太郎, 長澤康行, 伊藤誓悟

小児歯科学雑誌 62 ( 大会抄録号 ) 149 - 149 2024.4

ラットIgA腎症モデルにおける各種臓器での遺伝子発現の網羅的解析

松岡大貴, 末原佳奈, 仲周平, 野村良太, 仲野和彦, 仲野道代

小児歯科学雑誌 62 ( 大会抄録号 ) 153 - 153 2024.4

日本人小児口腔におけるStreptococcus mutansの陽性率と齲蝕経験歯数との関連の評価

奥田真琴, 末廣雄登, 東條文和, 又吉紗綾, 仲周平, 仲野道代, 仲野和彦

小児歯科学雑誌 62 ( 大会抄録号 ) 120 - 120 2024.4

非アルコール性脂肪肝炎患者口腔内から分離したStreptococcus mutansの各臓器における局在の検討

中野聡大, 田畑佳子, 仲周平, 仲野道代

小児歯科学雑誌 62 ( 地方会抄録号 ) 63 - 63 2024.2

廃棄される貝殻から生成したハイドロキシアパタイトのStreptococcus mutansに対する抑制効果メカニズムの検討

金木亜美, 小川将史, 臼田桃子, 亀谷茉莉子, 秋友達哉, 末廣雄登, 又吉紗綾, 仲周平, 大川玲奈, 光畑智恵子, 仲野道代, 仲野和彦, 野村良太, 八木正敏, 谷口泰造, 溝口敏郎, 藤川幸樹

小児歯科学雑誌 62 ( 地方会抄録号 ) 60 - 61 2024.2

Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β-1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci. International journal

Ryota Nomura, Yuto Suehiro, Fumikazu Tojo, Saaya Matayoshi, Rena Okawa, Masakazu Hamada, Shuhei Naka, Michiyo Matsumoto-Nakano, Rika Unesaki, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Tatsuya Akitomo, Chieko Mitsuhata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano

International journal of molecular sciences 25 ( 2 ) 2024.1

Language：English Publishing type：Research paper (scientific journal)

Shikonin is extracted from the roots of Lithospermum erythrorhizon, and shikonin extracts have been shown to have inhibitory effects on several bacteria. However, shikonin extracts are difficult to formulate because of their poor water solubility. In the present study, we prepared a shikonin dispersion, which was solubilized by the inclusion of β-1,3-1,6 glucan, and analysed the inhibitory effects of this dispersion on Streptococcus mutans and non-mutans streptococci. The shikonin dispersion showed pronounced anti-S. mutans activity, and inhibited growth of and biofilm formation by this bacterium. The shikonin dispersion also showed antimicrobial and antiproliferative effects against non-mutans streptococci. In addition, a clinical trial was conducted in which 20 subjects were asked to brush their teeth for 1 week using either shikonin dispersion-containing or non-containing toothpaste, respectively. The shikonin-containing toothpaste decreased the number of S. mutans in the oral cavity, while no such effect was observed after the use of the shikonin-free toothpaste. These results suggest that shikonin dispersion has an inhibitory effect on S. mutans and non-mutans streptococci, and toothpaste containing shikonin dispersion may be effective in preventing dental caries.

DOI： 10.3390/ijms25021075

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Diagnosis of maxillary lateral incisor dens invaginatus and therapeutic approach

Setsuyo Morimoto, Kana Goto, Asaumi Haruka, Tabata Keiko, Daiki Matsuoka, Shuhei Naka, Michiyo Matsumoto-Nakano

Pediatric Dental Journal 33 ( 3 ) 216 - 222 2023.12

非アルコール性脂肪肝炎患者から分離したStreptococcus mutansの増殖能に鉄イオンが及ぼす影響について

中野聡大, 田畑佳子, 仲周平, 仲野道代

岡山歯学会雑誌 42 ( 2 ) 78 - 78 2023.12

Porphyromonas gingivalis infection in the oral cavity is associated with elevated galactose-deficient IgA1 and increased nephritis severity in IgA nephropathy.

Seigo Ito, Taro Misaki, Yasuyuki Nagasawa, Ryota Nomura, Shuhei Naka, Akiko Fukunaga, Daiki Matsuoka, Saaya Matayoshi, Michiyo Matsumoto-Nakano, Kazuhiko Nakano

Clinical and experimental nephrology 2023.10

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: The relationship between the major periodontal bacteria, Porphyromonas gingivalis, and the pathogenesis of IgA nephropathy (IgAN)-particularly with respect to galactose-deficient IgA1 (Gd-IgA1)-has not been fully elucidated. METHODS: Saliva samples from 30 IgAN patients and 44 patients with chronic kidney disease (CKD) were subjected to analysis of P. gingivalis status via polymerase chain reaction using a set of P. gingivalis-specific primers. The associations between P. gingivalis presence and clinical parameters, including plasma Gd-IgA1, were analyzed in each group. RESULTS: Compared with the CKD group, the IgAN group demonstrated significantly higher plasma Gd-IgA1 levels (p < 0.05). Compared with the P. gingivalis-negative subgroup, the P. gingivalis-positive subgroup exhibited significantly higher plasma Gd-IgA1 levels in both IgAN and CKD patients (p < 0.05). Additionally, among IgAN patients, the P. gingivalis-positive subgroup displayed significantly higher plasma Gd-IgA1 and urine protein levels, compared with the P. gingivalis-negative subgroup (p < 0.05). With respect to renal biopsy findings, the frequencies of segmental glomerulosclerosis and tubular atrophy/interstitial fibrosis were significantly greater in the P. gingivalis-positive subgroup than in the P. gingivalis-negative subgroup, according to the Oxford classification of IgAN (p < 0.05). CONCLUSION: Our findings suggest an association between the presence of P. gingivalis in the oral cavity and the pathogenesis of IgAN, mediated by increased levels of Gd-IgA1.

DOI： 10.1007/s10157-023-02411-4

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Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite. International journal

Momoko Usuda, Mariko Kametani, Masakazu Hamada, Yuto Suehiro, Saaya Matayoshi, Rena Okawa, Shuhei Naka, Michiyo Matsumoto-Nakano, Tatsuya Akitomo, Chieko Mitsuhata, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano, Ryota Nomura

International journal of molecular sciences 24 ( 14 ) 2023.7

Language：English Publishing type：Research paper (scientific journal)

Hydroxyapatite adsorbs various substances, but little is known about the effects on oral bacteria of adsorption onto hydroxyapatite derived from scallop shells. In the present study, we analyzed the effects of adsorption of Streptococcus mutans onto scallop-derived hydroxyapatite. When scallop-derived hydroxyapatite was mixed with S. mutans, a high proportion of the bacterial cells adsorbed onto the hydroxyapatite in a time-dependent manner. An RNA sequencing analysis of S. mutans adsorbed onto hydroxyapatite showed that the upregulation of genes resulted in abnormalities in pathways involved in glycogen and histidine metabolism and biosynthesis compared with cells in the absence of hydroxyapatite. S. mutans adsorbed onto hydroxyapatite was not killed, but the growth of the bacteria was inhibited. Electron microscopy showed morphological changes in S. mutans cells adsorbed onto hydroxyapatite. Our results suggest that hydroxyapatite derived from scallop shells showed a high adsorption ability for S. mutans. This hydroxyapatite also caused changes in gene expression related to the metabolic and biosynthetic processes, including the glycogen and histidine of S. mutans, which may result in a morphological change in the surface layer and the inhibition of the growth of the bacteria.

DOI： 10.3390/ijms241411371

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Eruption cyst caused by congenital tooth in low birth weight infant

Eri Yoshida, Kana Goto, Daiki Matsuoka, Yukiko Miyai, Haruka Asaumi, Keiko Tabata, Shuhei Naka, Michiyo Matsumoto-Nakano

Pediatric Dental Journal 2023.7

口腔内のcnm遺伝子陽性S.mutansとIgA腎症患者の糖鎖異常IgA沈着の関連性

三崎太郎, 仲周平, 鈴木仁, 李明峰, 青木良輔, 長澤康行, 松岡大貴, 伊藤誓悟, 野村良太, 仲野道代, 鈴木祐介, 仲野和彦

日本腎臓学会誌 65 ( 3 ) 271 - 271 2023.5

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