Faculty Profiles - MAGARI Masaki

MISC - MAGARI Masaki

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4Ep16 Generation of antigen-specific antibodies using a chicken B cell line DT40 that expresses a human antibody

Kawakami Kanae, Inoue Kazue, Okayama Nobuhisa, Kanehiro Yuichi, Ikeda Mika, Magari Masaki, Omori Hitoshi, Kanayama Naoki

64 212 - 212 2012

4Ep14 Development of a novel protein display system using a hypermutating B cell line DT40

Hikasa Takuya, Matsuda Shuichi, Uetsuki Hidetomo, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

64 212 - 212 2012

Germinal center B cell differentiation and follicular dendritic cells.

Hitoshi Ohmori, Magari Magari, Naoki Kanayama

Clinical Immunology and Allergology 56 ( 2 ) 116 - 122 2011

AIDに依存した体細胞突然変異には,SRタンパク質SRSF1のスプライスアイソフォームが必要である

金山直樹, 金広優一, 藤堂景史, 根岸美咲, 福岡純司, 曲正樹, LI Xialu, MANLEY James L., 大森斉

生化学 2011

AID-dependen IgV hypermutation requires a splice isoform of the SR protein SRSF1

KANAYAMA Naoki, KANEHIRO Youichi, TODO Kagefumi, NEGISHI Misaki, FUKUOKA Junji, MAGARI Masaki, LI Xialu, MANLEY James L., OHMORI Hoitoshi

日本免疫学会総会・学術集会記録 40 2011

1S1p05 In vitro generation and affinity maturation of monoclonal antibodies using the hypermutating B cell line DT40

Kanayama Naoki, Magari Masaki, Ohmori Hitoshi

63 9 - 9 2011

3La04 Establishment of efficient methods for screening high-affinity antibodies from antibody library generated by using chicken B cell line DT40-SW

Ikeda Mika, Kojima Hiroyuki, Okazawa Takahiro, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

63 234 - 234 2011

Efficient in vitro antibody generation system using an engineered chicken B cell line, DT40-SW

Hitoshi Ohmori, Masaki Magari, Naoki Kanayama, Shuichi Matsuda, Yuichi Kanehiro

JOURNAL OF BIOTECHNOLOGY 150 S440 - S441 2010.11

変異能力を有するニワトリB細胞株DT40-SWを用いたマウスモノクローナル抗体の親和性成熟

金山直樹, 小島聡史, 藤井忍, 北村幸一, 井上知恵, 岡山展久, 松田修一, 鴨下佳代子, 藤堂景史, 池田美香, 曲正樹, 大森齊

日本生物工学会大会講演要旨集 62nd 2010

Creation of Valuable Antibodies by an In Vitro Antibody Generation System Using a Hypermutating B Cell Line

Naoki Kanayama, Kagefumi Todo, Masaki Magari, Hitoshi Ohmori

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 129 ( 1 ) 11 - 17 2009.1

Language：Japanese Publishing type：Book review, literature introduction, etc. Publisher：PHARMACEUTICAL SOC JAPAN

Monoclonal antibodies (mAb) have recently proven to be excellent biopharmaceutical agents. The generation of hybridomas from antigen-stimulated B cells has been a key technology for obtaining mAbs; however, it is a laborious and time-consuming process, and sometimes mAbs for molecules conserved between species are difficult to obtain because of immunological tolerance. Thus, it is of great importance to develop in vitro technologies for generating useful Abs as drug candidates. We have been attempting to develop a novel in vitro antibody generation system using a chicken B cell line DT40, which displays Abs and mutates Ig genes during Culture, thereby generating a useful Ab library for screening mAbs. First, we generated an engineered cell line DT40-SW whose mutation machinery can be reversibly switched on and off. The Ab generation system using DT40-SW is useful in the following ways: (1) mAbs for various model antigens including antoantigens can be obtained from the DT40-SW Ab library that is free from immunological tolerance; (2) the switching device of the mutation machinery enables fixing desirable Ig mutants by stopping mutation; (3) by repeated culture and sorting of clones bearing higher affinity for target antigens, affinity maturation can be mimicked in vitro. We have also genetically improved DT40-SW cells for mutation efficiency and Ab production. The Ab generation system will be applicable for obtaining valuable Abs such as antitumor Abs.

DOI： 10.1248/yakushi.129.11

Web of Science

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2Ep15 Analysis of regulatory mechanism for AID-dependent Ig gene mutation in a chicken B cell line

NEGISHI Misaki, UNE Yukiko, KANEHIRO Yuichi, MAGARI Masaki, KANAYAMA Naoki, OHMORI Hitoshi

21 64 - 64 2009