Faculty Profiles - MAGARI Masaki

MISC - MAGARI Masaki

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CaMKKの基質認識機構の解明と特異的阻害分子開発への応用

川俣一晟, 美馬光志, 山内陽生, 北前勝哉, 山内香奈, 大塚里美, 曲正樹, 金山直樹, 徳光浩

日本生化学会大会(Web) 95th 2022

CaMKK阻害剤(TIM-063)を用いた阻害剤プロテオミクス解析

大塚里美, 波多野直哉, 奥村太晟, 澤直樹, 田邊史子, 傳田美和子, 金山直樹, 曲正樹, 森下了, 石川彰彦, 徳光浩

日本生化学会大会(Web) 94th 2021

ヘルスシステムの理解とその応用濾胞樹状細胞による抗体の親和性成熟の制御機構の解明(Interdisciplinary Science and Engineering in Health Systems Immunological functions of follicular dendritic cells on affinity maturation of antibody)

Magari Masaki, Ogawa Sayaka, Matsuoka Yukiko, Takada Miho, Kanayama Naoki, Tokumitsu Hiroshi

生物物理 58 ( Suppl.1-2 ) S197 - S197 2018.8

Analysis of a role for SRSF1-3 in a transcription-coupled process during IgV hypermutation

Y. Kawaguchi, K. Yokoyama, N. Kawamoto, H. Nariki, Y. Matsuyama, Y. Kanehiro, T. Hikasa, M. Magari, H. Ohmori, H. Tokumitsu, N. Kanayama

EUROPEAN JOURNAL OF IMMUNOLOGY 46 1267 - 1268 2016.8

SRSF1-3 has a role in nuclear localization of AID by regulating its nuclear export

N. Kanayama, Y. Kawaguchi, N. Kawamoto, H. Nariki, S. Miyazaki, K. Yokoyama, M. Magari, H. Ohmori, H. Tokumitsu

EUROPEAN JOURNAL OF IMMUNOLOGY 46 1142 - 1142 2016.8

IL-34-dependent differentiation of monocytic cell with B cell stimulating activity

M. Magari, S. Ogawa, Y. Toya, K. Hieda, F. Yamane, N. Kanayama, H. Tokumitsu, H. Ohmori

EUROPEAN JOURNAL OF IMMUNOLOGY 46 1139 - 1139 2016.8

2P-071 Enhanced production of humanized antibody in the chicken B cell line DT40

Nakatani Koji, Danjo Satoshi, Magari Masaki, Tokumitsu Hiroshi, Knayama Naoki

67 192 - 192 2015

2P-070 High-affinity antibody generation by regulating BCR signal dependent apoptosis in the chicken B cell line

Kanayama Naoki, Koga Mai, Ikeda Mika, Kojima Hiroyuki, Magari Masaki, Tokumitsu Hiroshi, Ohmori Hitoshi

67 192 - 192 2015

Ca²⁺/Calmodulin結合型転写因子の網羅的同定

太尾田泰成, 大西和貴, 古谷雄穂, 傳田美和子, 金山直樹, 曲正樹, 森下了, 徳光浩

日本生化学会大会(Web) 88th 2015

インターラクトーム解析法を用いたヒトS100A6標的分子の探索

坂根恭平, 西口みゆ, 古谷雄穂, 傳田美和子, 山口文徳, 曲正樹, 金山直樹, 森下了, 徳光浩

日本生化学会大会(Web) 88th 2015

Role for serine/arginine-rich protein splicing factor (SRSF) in somatic hypermutaion.

Masaki Magari, Naoki Kanayama

Clinical Immunology and Allergology 61 ( 5 ) 489 - 495 2014

Induction of somatic hypermutation on the immunoglobulin gene by AID and related molecules.

Naoki Kanayama, Masaki Magari

Clinical Immunology and Allergology 61 ( 5 ) 467 - 471 2014

3P-032 Engineering of antibodies using the hypermutating B cell line

Kanayama Naoki, Watanabe Koji, Koga Mai, Kawakami Kanae, Uetsuki Hidetomo, Tokumitsu Hiroshi, Magari Masaki, Ohmori Hitoshi

66 202 - 202 2014

ヒトCalmodulin標的分子の網羅的同定

古谷雄穂, 西口みゆ, 傳田美和子, 曲正樹, 金山直樹, 森下了, 徳光浩

日本生化学会大会(Web) 87th 2014

A missense mutation in Rev7 disrupts formation of Pol zeta and impairs mouse development and replication dependent ICL DNA repair

M. Khalaj, A. Abbasi, H. Yamanishi, K. Akiyama, S. Kikuchi, M. Hirose, M. Yuzuriha, M. Magari, H. Degheidy, K. Abe, A. Ogura, H. Hashimoto, T. Kunieda

MOLECULAR BIOLOGY OF THE CELL 24 2013

3P-002 High-affinity antibody generation using the chicken B cell line DT40-SW by regulating B cell receptor-mediated apoptosis

Koga Mai, Ikeda Mika, Kojima Hiroyuki, Kawakami Kanae, Watanabe Koji, Tokumitsu Hiroshi, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

65 188 - 188 2013

3P-003 Protein evolution in a protein display system using the hypermutating B cell line DT40-SW

Watanabe Koji, Hikasa Takuya, Uetsuki Hidetomo, Tokumitsu Hiroshi, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

65 189 - 189 2013

Role for follicular dendritic cells in the negative selection of germinal center B cells.

Masaki Magari, Naoki Kanayama, Hitoshi Ohmori

Clinical Immunology and Allergology 58 ( 3 ) 259 - 265 2012

DT40細胞株を用いたin vitro抗体作製システムにおける,抗原レセプターの刺激に依存した生存による抗原特異的抗体産生細胞の選択法の開発

渡邊康二, 藤堂景史, 福岡純司, 曲正樹, 大森齊, 金山直樹

日本分子生物学会年会プログラム・要旨集(Web) 35th 2012

4Ep15 Affinity maturation of monoclonal antibodies in the hypermutating chicken B cell line DT40-SW

Sai En, Kojima Satoshi, Fujii Shinobu, Kitamura Kouichi, Kamoshita Kayoko, Ikeda Mika, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

64 212 - 212 2012

4Ep16 Generation of antigen-specific antibodies using a chicken B cell line DT40 that expresses a human antibody

Kawakami Kanae, Inoue Kazue, Okayama Nobuhisa, Kanehiro Yuichi, Ikeda Mika, Magari Masaki, Omori Hitoshi, Kanayama Naoki

64 212 - 212 2012

4Ep14 Development of a novel protein display system using a hypermutating B cell line DT40

Hikasa Takuya, Matsuda Shuichi, Uetsuki Hidetomo, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

64 212 - 212 2012

Germinal center B cell differentiation and follicular dendritic cells.

Hitoshi Ohmori, Magari Magari, Naoki Kanayama

Clinical Immunology and Allergology 56 ( 2 ) 116 - 122 2011

AIDに依存した体細胞突然変異には,SRタンパク質SRSF1のスプライスアイソフォームが必要である

金山直樹, 金広優一, 藤堂景史, 根岸美咲, 福岡純司, 曲正樹, LI Xialu, MANLEY James L., 大森斉

生化学 2011

AID-dependen IgV hypermutation requires a splice isoform of the SR protein SRSF1

KANAYAMA Naoki, KANEHIRO Youichi, TODO Kagefumi, NEGISHI Misaki, FUKUOKA Junji, MAGARI Masaki, LI Xialu, MANLEY James L., OHMORI Hoitoshi

日本免疫学会総会・学術集会記録 40 2011

1S1p05 In vitro generation and affinity maturation of monoclonal antibodies using the hypermutating B cell line DT40

Kanayama Naoki, Magari Masaki, Ohmori Hitoshi

63 9 - 9 2011

3La04 Establishment of efficient methods for screening high-affinity antibodies from antibody library generated by using chicken B cell line DT40-SW

Ikeda Mika, Kojima Hiroyuki, Okazawa Takahiro, Magari Masaki, Ohmori Hitoshi, Kanayama Naoki

63 234 - 234 2011

Efficient in vitro antibody generation system using an engineered chicken B cell line, DT40-SW

Hitoshi Ohmori, Masaki Magari, Naoki Kanayama, Shuichi Matsuda, Yuichi Kanehiro

JOURNAL OF BIOTECHNOLOGY 150 S440 - S441 2010.11

変異能力を有するニワトリB細胞株DT40-SWを用いたマウスモノクローナル抗体の親和性成熟

金山直樹, 小島聡史, 藤井忍, 北村幸一, 井上知恵, 岡山展久, 松田修一, 鴨下佳代子, 藤堂景史, 池田美香, 曲正樹, 大森齊

日本生物工学会大会講演要旨集 62nd 2010

Creation of Valuable Antibodies by an In Vitro Antibody Generation System Using a Hypermutating B Cell Line

Naoki Kanayama, Kagefumi Todo, Masaki Magari, Hitoshi Ohmori

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 129 ( 1 ) 11 - 17 2009.1

Language：Japanese Publishing type：Book review, literature introduction, etc. Publisher：PHARMACEUTICAL SOC JAPAN

Monoclonal antibodies (mAb) have recently proven to be excellent biopharmaceutical agents. The generation of hybridomas from antigen-stimulated B cells has been a key technology for obtaining mAbs; however, it is a laborious and time-consuming process, and sometimes mAbs for molecules conserved between species are difficult to obtain because of immunological tolerance. Thus, it is of great importance to develop in vitro technologies for generating useful Abs as drug candidates. We have been attempting to develop a novel in vitro antibody generation system using a chicken B cell line DT40, which displays Abs and mutates Ig genes during Culture, thereby generating a useful Ab library for screening mAbs. First, we generated an engineered cell line DT40-SW whose mutation machinery can be reversibly switched on and off. The Ab generation system using DT40-SW is useful in the following ways: (1) mAbs for various model antigens including antoantigens can be obtained from the DT40-SW Ab library that is free from immunological tolerance; (2) the switching device of the mutation machinery enables fixing desirable Ig mutants by stopping mutation; (3) by repeated culture and sorting of clones bearing higher affinity for target antigens, affinity maturation can be mimicked in vitro. We have also genetically improved DT40-SW cells for mutation efficiency and Ab production. The Ab generation system will be applicable for obtaining valuable Abs such as antitumor Abs.

DOI： 10.1248/yakushi.129.11

Web of Science

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2Ep15 Analysis of regulatory mechanism for AID-dependent Ig gene mutation in a chicken B cell line

NEGISHI Misaki, UNE Yukiko, KANEHIRO Yuichi, MAGARI Masaki, KANAYAMA Naoki, OHMORI Hitoshi

21 64 - 64 2009

2Ep16 A novel antibody screening system using a chicken B cell line, DT40-SW : switching system of gene conversion and point mutation

MAGARI Masaki, KAJITA Masamichi, KANAYAMA Naoki, OHMORI Hitoshi

21 65 - 65 2009

2Ep17 In vitro antibody screening system using a chicken B cell line ,DT40-SW : application of converting mutation pattern for high affinity antibodies

KAJITA Masamichi, MAGARI Masaki, TODO Kagefumi, KANAYAMA Naoki, OHMORI Hitoshi

21 65 - 65 2009

変異能力を有するニワトリB細胞株を用いた抗体の親和性成熟:外来の抗体可変部遺伝子のDT40-SWにおける発現・改良系の構築

小島聡史, 藤井忍, 北村幸一, 井上知恵, 岡山展久, 松田修一, 藤堂景史, 曲正樹, 金山直樹, 大森斉

日本分子生物学会年会講演要旨集 32nd ( Vol.1 ) 2009

変異能力を有するニワトリB細胞株を用いた抗体の親和性成熟:変異様式の変換による親和性成熟の効率化

梶田真道, 曲正樹, 池田美香, 藤堂景史, 金山直樹, 大森斉

日本分子生物学会年会講演要旨集 32nd ( Vol.1 ) 2009

変異能力を有するニワトリB細胞株を用いた抗体の親和性成熟:DT40-SWを用いたマウスモノクローナル抗体の改変

金山直樹, 小島聡史, 藤井忍, 北村幸一, 井上知恵, 岡山展久, 松田修一, 藤堂景史, 曲正樹, 大森斉

日本分子生物学会年会講演要旨集 32nd ( Vol.1 ) 2009

抗体医薬が切り拓く先端医療培養B細胞株を用いるin vitro抗体作製システムによる有用抗体の創製

金山直樹, 藤堂景史, 曲正樹, 大森齊

日本薬学会年会要旨集 128年会 ( 1 ) 134 - 134 2008.3

3Ca13 Selective activation of antigen-specific B cells in vitro by FDC-like line; FL-Y.

MAGARI Masaki, NISHIKAWA Yumiko, SANO Hiroki, FUJII Yasumasa, KANAYAMA Naoki, OHMORI Hitoshi

20 109 - 109 2008

ニワトリB細胞株DT40-SWを用いるin vitro抗体作製システムの開発:Pax5発現抑制による抗体産生の増強

曲正樹, 綾高宏, 藤堂景史, 金山直樹, 大森斉

日本生物工学会大会講演要旨集 59th 2007

1K16-1 Generation of monoclonal antibodies by a novel antibody screening system using a hypermutating B cell line

TODO Kagefumi, MIYAKE Kenji, FUJITA Risako, OKAZAWA Takahiro, IKEDA Mika, MAGARI Masaki, KANAYAMA Naoki, OHMORI Hitoshi

18 197 - 197 2006

1K16-2 Construction of an antibody library from a chicken B cell line, DT40 by controlling point mutation and gene conversion

KAJITA Masamichi, TODO Kagefumi, MAGARI Masaki, KANAYAMA Naoki, OHMORI Hitoshi

18 197 - 197 2006

抗体遺伝子変換機構を利用した非抗体タンパクのDNAシャッフリングによる機能改変

藤堂景史, 高橋佐都子, 片岡大輔, 曲正樹, 金山直樹, 大森斉

日本免疫学会総会・学術集会記録 35 2005

B細胞の遺伝子変換機構を利用した非抗体タンパクのDNAシャッフリングによる機能改変

藤堂景史, 高橋佐都子, 片岡大輔, 綱島良太, 曲正樹, 金山直樹, 大森斉

日本分子生物学会年会講演要旨集 28th 2005

B細胞の遺伝子変換機構を利用する非抗体タンパクの機能改変

藤堂景史, 高橋佐都子, 片岡大輔, 曲正樹, 金山直樹, 大森斉

日本生物工学会大会講演要旨集 2005 2005

高頻度変異機能のON/OFF制御可能なB細胞株を用いるin vitro抗体作製システム

金山直樹, 藤堂景史, 三宅健司, 曲正樹, 大森斉

日本分子生物学会年会講演要旨集 28th 2005

B細胞レパトアの限定されたマウスにおけるB細胞選択と親和性成熟の解析

金山直樹, 木本崇文, 藤堂景史, 曲正樹, 大森齊

生化学 76 ( 3 ) 306 - 306 2004.3

2H15-3 Analysis of antibody affinity maturation by single cell PCR

KANAYAMA Naoki, OKAZAWA Takahiro, KOYAMA Emi, MAGARI Masaki, OHMORI Hitoshi

16 215 - 215 2004

ニワトリB細胞株を用いたタンパク分子改変系の構築:変異導入機能のON/OFF制御

藤堂景史, 曲正樹, 金山直樹, 大森斉

日本生物工学会大会講演要旨集 2004 2004

ニワトリB細胞株を用いたタンパク分子改変系の構築:外来遺伝子の遺伝子変換

高橋佐都子, 藤堂景史, 曲正樹, 金山直樹, 大森斉

日本生物工学会大会講演要旨集 2004 2004

Signal intensity via B cell antigen receptor and B cell responses

Masaki Magari, Naoki Kanayama, Hitoshi Ohmori

Clinical Immunology 40 ( 4 ) 454 - 457 2003

Conversion and expression of foreign genes in the chicken B cell line

Todo Kagefumi, Takahashi Satoko, Magari Masaki, Kanayama Naoki, Ohmori Hitoshi

15 87 - 87 2003

Analysis of affinity maturation by single-cell PCR

Okazawa Takahiro, Magari Masaki, Kimoto Takafumi, Kouyama Emi, Kanayama Naoki, Ohmori Hitoshi

15 187 - 187 2003

B細胞レパトアの限定されたマウスを用いるB細胞選択と親和性成熟の解析

木本崇文, 松浦正明, 藤堂景史, 曲正樹, 疋田正喜, 金山直樹, 大森齊

生化学 74 ( 8 ) 949 - 949 2002.8

B細胞多様性の限定されたマウスにおけるクローン選択と親和性成熟の解析

木本崇文, 藤堂景史, 疋田正喜, 曲正樹, 金山直樹, 大森斉

日本免疫学会総会・学術集会記録 32 2002

B細胞多様性の限定されたマウスにおける抗体の親和性成熟機構の解析

藤堂景史, 木本崇文, 曲正樹, 金山直樹, 大森斉

日本生物工学会大会講演要旨集 2002 2002

1130 Detection of RAG expressed cells by a new fluorescence-labeling mothod

Miki Takao, Nishikawa Emiko, Magari Masaki, Hikida Masaki, Kanayama Masaki, Ohmori Hitoshi

14 206 - 206 2002

Analysis of affinity maturation in quasi monoclonal mouse

Kimoto Takafumi, Matsuura Masaaki, Magari Masaki, Kanayama Naoki, Hikida Masaki, Ohmori Hitoshi

13 341 - 341 2001

Analysis of affinity maturation of antibody using Ig-transgenic mice.

Magari Masaki, Sawatari Takashi, Kanayama Naoki, Hikida Masaki, Ohmori Hitoshi

11 139 - 139 1999

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MISC - MAGARI Masaki