2021/05/07 更新

写真a

オゴシ マホ
御輿 真穂
OGOSHI Maho
所属
自然科学学域 助教
職名
助教
外部リンク

研究キーワード

  • イソトシン

  • トビハゼ

  • 国際研究者交流

  • 環境適応

  • 神経行動

  • バソトシン

  • アドレノメデュリン

  • 脊椎動物

  • 比較内分泌学

研究分野

  • ライフサイエンス / 進化生物学

  • ライフサイエンス / 形態、構造

経歴

  • 岡山大学   学術研究院自然科学学域   助教

    2021年4月 - 現在

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  • 岡山大学   大学院自然科学研究科   助教

    2013年4月 - 2021年3月

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論文

  • Adrenomedullin 2 and 5 activate the calcitonin receptor-like receptor (clr) - Receptor activity-modifying protein 3 (ramp3) receptor complex in Xenopus tropicalis. 国際誌

    Maho Ogoshi, Mikoto Takahashi, Kota Aoyagi, Kazuyoshi Ukena, Sayaka Aizawa, Hideaki Takeuchi, Sumio Takahashi, Sakae Takeuchi

    General and comparative endocrinology306   113752 - 113752   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The adrenomedullin (AM) family is involved in diverse biological functions, including cardiovascular regulation and body fluid homeostasis, in multiple vertebrate lineages. The AM family consists of AM1, AM2, and AM5 in tetrapods, and the receptor for mammalian AMs has been identified as the complex of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 2 (RAMP2) or RAMP3. However, the receptors for AM in amphibians have not been identified. In this study, we identified the cDNAs encoding calcrl (clr), ramp2, and ramp3 receptor components from the western clawed frog (Xenopus tropicalis). Messenger RNAs of amphibian clr and ramp2 were highly expressed in the heart, whereas that of ramp3 was highly expressed in the whole blood. In HEK293T cells expressing clr-ramp2, cAMP response element luciferase (CRE-Luc) reporter activity was activated by am1. In HEK293T cells expressing clr-ramp3, CRE-Luc reporter activity was increased by the treatment with am2 at the lowest dose, but with am5 and am1 at higher dose. Our results provided new insights into the roles of AM family peptides through CLR-RAMP receptor complexes in the tetrapods.

    DOI: 10.1016/j.ygcen.2021.113752

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  • Ghrelin and food acquisition in wild and cultured Japanese eel (Anguilla japonica). 国際誌

    Takashi Yada, Michihisa Abe, Kenzo Kaifu, Kazuki Yokouchi, Nobuto Fukuda, Sakie Kodama, Hiroshi Hakoyama, Maho Ogoshi, Hiroyuki Kaiya, Tatsuya Sakamoto, Shunsuke Moriyama, Katsumi Tsukamoto

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology245   110700 - 110700   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To clarify the relationships between growth, endocrine status and habitat characteristics in Japanese eel (Anguilla japonica), plasma and stomach mRNA levels of ghrelin were examined in wild eels captured in the river and the bay, and in cultured eels during and after experimental fasting. Wild juvenile eels captured in freshwater habitats within the river showed significantly higher plasma and stomach mRNA levels of ghrelin than did fish obtained from brackish-water habitats within the bay. In cultured eels experimentally fasted for 4 weeks, plasma and stomach mRNA levels of ghrelin increased. After refeeding, the both parameters returned to the levels observed in continuously feeding control fish. In pigmented elvers, 2 months of feed restriction resulted in a significant increase in whole-body ghrelin mRNA. It is suggested that interaction between ghrelin and feeding is related to their habitats through differential food acquisition in fresh and brackish water environments.

    DOI: 10.1016/j.cbpa.2020.110700

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  • Adenosine stimulates neuromedin U mRNA expression in the rat pars tuberalis. 査読 国際誌

    Sayaka Aizawa, Tingting Gu, Arisa Kaminoda, Ryuya Fujioka, Fumiya Ojima, Ichiro Sakata, Takafumi Sakai, Maho Ogoshi, Sumio Takahashi, Sakae Takeuchi

    Molecular and cellular endocrinology496 ( 496 ) 110518 - 110518   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Neuromedin U (NMU) shows circadian expression in the rat pars tuberalis (PT), and is known to be suppressed by melatonin. Here we examined the involvement of adenosine in the regulation of Nmu expression. We found that the rat PT expressed adenosine receptor A2b and that an adenosine receptor agonist, NECA, stimulated Nmu expression in brain slice cultures. In vitro promoter assays revealed that NECA stimulated Nmu promoter activity via a cAMP response element (CRE) in the presence of adenosine receptor A2b. NECA also increased the levels of phosphorylated CRE-binding protein. These findings suggest that adenosine stimulates Nmu expression by activating the cAMP signaling pathway through adenosine receptor A2b in the rat PT. This is the first report to demonstrate that Nmu expression in the PT is regulated by adenosine, which acts as an intravital central metabolic signal, in addition to melatonin, which acts as an external photoperiodic environmental signal.

    DOI: 10.1016/j.mce.2019.110518

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  • Runx3 regulates folliculogenesis and steroidogenesis in granulosa cells of immature mice. 査読 国際誌

    Fumiya Ojima, Yuka Saito, Yukiko Tsuchiya, Maho Ogoshi, Hiroshi Fukamachi, Kenichi Inagaki, Fumio Otsuka, Sakae Takeuchi, Sumio Takahashi

    Cell and tissue research375 ( 3 ) 743 - 754   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously demonstrated that female Runx3 knockout (Runx3-/-) mice were anovulatory and their uteri were atrophic and that Runx3 mRNA was expressed in granulosa cells. To clarify how Runx3 regulates folliculogenesis and ovulation, we examine the effects of Runx3 knockout on the gene expression of growth factors associated with folliculogenesis and enzymes associated with steroidogenesis. In Runx3-/- mouse ovaries, the numbers of primary and antral follicles were lower than those in wild-type (wt) mice at 3 weeks of age, indicating that the loss of Runx3 affects folliculogenesis. The expression of genes encoding activin and inhibin subunits (Inha, Inhba and Inhbb) was also decreased in ovaries from the Runx3-/- mice compared with that in wt mice. Moreover, the expression of the genes Cyp11a1 and Cyp19a1 encoding steroidogenic enzymes was also decreased. In cultured granulosa cells from 3-week-old mouse ovaries, Cyp19a1 mRNA levels were lower in Runx3-/- mice than those in wt mice. Follicle-stimulating hormone (FSH) treatment increased Cyp19a1 mRNA levels in both wt and Runx3-/- granulosa cells in culture but the mRNA level in Runx3-/- granulosa cells was lower than that in wt ones, indicating that granulosa cells could not fully function in the absence of Runx3. At 3 weeks of age, gonadotropin α subunit, FSHβ subunit and luteinizing hormone (LH) β subunit mRNA levels were decreased in Runx3-/- mice. These findings suggest that Runx3 plays a key role in female reproduction by regulating folliculogenesis and steroidogenesis in granulosa cells.

    DOI: 10.1007/s00441-018-2947-2

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  • Changes in prolactin receptor homodimer availability may cause late feathering in chickens. 国際誌

    Ayako Okamura, Ayane Masumoto, Atsushi Takenouchi, Toshiyuki Kudo, Sayaka Aizawa, Maho Ogoshi, Sumio Takahashi, Masaoki Tsudzuki, Sakae Takeuchi

    General and comparative endocrinology272   109 - 116   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chicken early (EF) and late feathering (LF) are sex-linked phenotypes conferred by wild-type k+ and dominant K alleles on chromosome Z, respectively. Besides prolactin (PRL) receptor (PRLR) and sperm flagellar 2 (SPEF2) genes, the K allele contains a fusion gene in which partially duplicated PRLR (dPRLR) and SPEF2 (dSPEF2) genes are linked in a tail-to-tail manner. The causative dPRLR gene encodes a C-terminal truncated receptor. LF chickens have short or no primaries at hatching; however, their feather growth rate is higher than that of EF chickens. This study aimed to elucidate the molecular basis of the K allele's biphasic effect on feather development. By 3'RACE and RT-PCR analyses, we demonstrated that dSPEF2 gene transcription occurred beyond all coding exons of the dPRLR gene on the opposite strand and that dPRLR mRNA was less abundant than PRLR mRNA. In addition, a 5'UTR splice variant (SPV) of PRL receptor mRNAs was increased in LF chickens. In vitro expression analysis of 5'UTR linked to the luciferase reporter gene revealed higher translation efficiency of SPV. RT-qPCR showed that the dPRLR mRNA level was higher in embryos; conversely, SPV was higher in hatched chickens, as was dSPEF2 mRNA. These findings suggest that the K allele inhibits feather development at the fetal stage by expressing dPRLR to attenuate PRLR function and promotes feather growth after hatching by increasing PRLR through dSPEF2 mRNA expression. Increased SPV may cause greater feather growth than that in EF chickens by increasing the availability of PRLR homodimers and enhancing PRL signaling.

    DOI: 10.1016/j.ygcen.2018.12.011

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  • Runx3 transcription factor regulates ovarian functions and ovulation in female mice. 査読

    Fumiya Ojima, Yuka Saito, Yukiko Tsuchiya, Daichi Kayo, Syusuke Taniuchi, Maho Ogoshi, Hiroshi Fukamachi, Sakae Takeuchi, Sumio Takahashi

    The Journal of reproduction and development62 ( 5 ) 479 - 486   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously demonstrated that the Runx3 transcription factor is expressed in the hypothalami, pituitaries, and ovaries of mice, and that Runx3 knockout (Runx3-/-) mice are anovulatory and their uteri are atrophic. Runx3 mRNA expression was detected in the granulosa cells of ovarian follicles, and in the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC). In the present study, we examined the effects of Runx3 knockout on the gene expression of enzymes associated with steroidogenesis. We found decreased Cyp11a1 mRNA expression in Runx3-/- mouse ovaries compared with that in wild-type (wt) mouse ovaries at the age of 8 weeks. In situ hybridization analysis showed that the percentages of Cyp11a1 mRNA-expressing theca cells in follicles of Runx3-/- mice were decreased compared with those of wt mice. In accord with the alterations in Runx3-/- mouse ovaries, Kiss1 mRNA levels in ARC were increased, whereas mRNA levels of kisspeptin in AVPV were decreased, and gonadotropin-releasing hormone in the preoptic area and follicle-stimulating hormone β subunit gene were increased in Runx3-/- mice. Following an ovarian transplantation experiment between Runx3-/- mice and wt mice, corpora lutea were observed when ovaries from Runx3-/- mice were transplanted into wt mice, but not when those from wt mice were transplanted into Runx3-/- mice, suggesting that Runx3 in the hypothalamo-pituitary system may drive gonadotropin release to induce ovulation in the ovary. These findings indicate that Runx3 plays a crucial role in the hypothalamo-pituitary-gonadal axis.

    DOI: 10.1262/jrd.2016-005

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  • Functional characterization of the mouse melanocortin 3 receptor gene promoter. 査読 国際誌

    Keisuke Okutsu, Fumiya Ojima, Naoto Shinohara, Shusuke Taniuchi, Yasusyo Mizote, Kenji Aoki, Toshiyuki Kudo, Maho Ogoshi, Sakae Takeuchi, Sumio Takahashi

    Gene562 ( 1 ) 62 - 9   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Melanocortin receptor 3 (MC3R) is expressed in the hypothalamus and pituitary in humans and rodents, and is involved in the control of feeding, energy metabolism, and pituitary function. In the mouse pituitary, MC3R is detected in mammotrophs. This study aimed to clarify the regulatory mechanism for Mc3r expression in the mouse pituitary. The promoter activities of reporter constructs for the MC3R gene 5'-flanking region up to -4000 bp (transcription initiation site designated as +1) were analyzed. The promoter activity significantly increased in the -86/+109 construct, but decreased in the -38/+109 construct, indicating that the minimal promoter required for basal expression of Mc3r is located in the -86/+109 region. Putative binding sites for transcription factors AP-1 and ATF4 were found in the 5'-flanking region of Mc3r. Site-directed mutation or deletion of these sites affected the promoter activities. In gel-shift assays with a nuclear extract of mouse anterior pituitary cells, band-shifts were detected for both sites after the addition of the nuclear extract, and were decreased in the presence of excess unlabeled probe competitors. These results indicated that both sites were involved in the regulation of Mc3r expression in anterior pituitary cells. Estradiol-17β treatment increased the Mc3r promoter activity, indicating that the gene is regulated by estradiol-17β. In conclusion, we have demonstrated the minimum promoter region required for Mc3r expression, and identified two binding sites for AP-1 and ATF4 and in the 5' upstream-flanking region of Mc3r that are essential for Mc3r expression.

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  • Effect of environmental salinity on expression of adrenomedullin genes suggests osmoregulatory activity in the medaka, Oryzias latipes. 査読 国際誌

    Maho Ogoshi, Kanoko Kato, Tatsuya Sakamoto

    Zoological letters1   12 - 12   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: The adrenomedullins (AMs) comprise a hormonal family in mammals and teleost fishes, with five members (AM1-5) found or predicted in most of the teleosts including Japanese medaka (Oryzias latipes). AM1 is known to have cardiovascular and osmoregulatory functions in mammals, but the roles of most AMs are yet to be determined. RESULTS: Using medaka, we first analyzed the tissue distribution of all five AM genes and found detectable expression in all tissues examined, with relatively high levels of AM3 and AM5 in the liver and kidney. To assess the osmoregulatory roles of these AMs, mRNA levels were examined in the brain (including the eyes), gill, liver, kidney and spleen of medaka one week after transfer from isotonic saline (11 ppt) to freshwater (0 ppt) or seawater (33 ppt). Expression of AM1 in the brain-eye increased in freshwater. The central level of AM4 (the paralog of AM1) decreased in seawater; the branchial level of AM4 decreased in freshwater and seawater, but the renal level increased in freshwater. The branchial level of AM2 increased in seawater, whereas the renal level decreased in freshwater and seawater. Expression of AM3, the AM2 paralog, decreased in the brain-eye of seawater-acclimated fish. Expression of AM5 in the brain-eye and kidney decreased in seawater. CONCLUSIONS: Except for branchial AM2, the members of AM family tend to be involved in promotion of hyper-osmoregulation and/or inhibition of hypo-osmoregulation, although each AM may play a distinct role during adaptation to different salinities.

    DOI: 10.1186/s40851-015-0012-5

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  • Identification of a feather β-keratin gene exclusively expressed in pennaceous barbule cells of contour feathers in chicken. 査読 国際誌

    Kowata K, Nakaoka M, Nishio K, Fukao A, Satoh A, Ogoshi M, Takahashi S, Tsudzuki M, Takeuchi S

    Gene542 ( 1 ) 23 - 8   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.gene.2014.03.027

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  • Exploring new CGRP family peptides and their receptors in vertebrates. 査読 国際誌

    Yoshio Takei, Maho Ogoshi, Shigenori Nobata

    Current protein & peptide science14 ( 4 ) 282 - 93   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BENTHAM SCIENCE PUBL LTD  

    Vertebrates have expanded their habitats from aquatic to terrestrial environments, which has accompanied the evolution of cardiovascular and osmoregulatory hormones. Specifically, mammals have developed mechanisms to maintain high blood pressure and blood volume, while extant fishes have developed hypotensive and Na-extruding mechanisms to adapt to the marine environment where they underwent a vast diversification. The CGRP family is one of the hormone systems that decrease blood pressure and blood volume. Within the CGRP family of teleost fishes, we found that adrenomedullins (AMs) have diversified and five paralogs (AM1-5) form an independent subfamily. Based on this discovery in fishes, we found AM2 and AM5 in mammals. In mammalian species that have AM2 and/or AM5, the peptides assume greater importance in the case of pathophysiological disturbances in pressure and fluid balance such as hypertension and cardiac and renal failure. In addition, novel functions of AM peptides have been suggested by the discovery of AM2 and AM5 in mammals. Current research on the CGRP family is focused on the identification of new receptors for AM2/AM5 and the establishment of AM2 knockout mice, which will enable new developments in the basic and clinical research on this intriguing hormone family. Importantly, comparative fish studies can contribute to new developments in our understanding of the function of the AM peptides.

    DOI: 10.2174/13892037113149990048

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  • Growth, energetics and the cortisol-hepatic glucocorticoid receptor axis of medaka (Oryzias latipes) in various salinities. 査読 国際誌

    Maho Ogoshi, Kanoko Kato, Hideya Takahashi, Toshitaka Ikeuchi, Tsukasa Abe, Tatsuya Sakamoto

    General and comparative endocrinology178 ( 2 ) 175 - 9   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    We examined growth of euryhaline Japanese medaka (Oryzias latipes) after transfer to freshwater or seawater from isotonic saline. Growth was unaffected by the different salinities for 1 week, but the body weight increase and BMI of fish kept in freshwater for 2-3 weeks were significantly higher than those in the isotonic controls. These results may reflect the usual habitat of this species. To assess the basis for the difference in growth, energetics and the hepatic stress axis were evaluated 1 week after the transfer. Unexpectedly, despite the higher growth rate, the rate of routine oxygen consumption was significantly higher in freshwater. Plasma cortisol levels in freshwater were significantly higher than those in seawater, and the mRNA levels of the glucocorticoid receptor (GR1) in the liver were significantly lower in freshwater and seawater, compared to that in isotonic saline. Branchial Na(+)/K(+)-ATPase activities were also reduced significantly in freshwater and seawater, compared to that in isotonic saline. The higher levels of hepatic GR1 expression and branchial Na(+)/K(+)-ATPase activity in isotonic salinity than those in freshwater and seawater for 1 week may account for the lower growth rate under the isotonic condition. After 3 weeks, however, the Na(+)/K(+)-ATPase activity in seawater was significantly higher than that in freshwater. No significant difference in growth rate between freshwater and seawater groups indicates that medaka is a good model for studies of hypo- and hyperosmotic adaptations, since osmoregulation is not strongly associated with size and growth.

    DOI: 10.1016/j.ygcen.2012.05.001

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  • Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: possible physiological roles of the calcitonin family in osmoregulation. 査読 国際誌

    Nobuo Suzuki, Toshio Sekiguchi, Honoo Satake, Kanoko Kato, Yudai Nishiyama, Hideya Takahashi, Janine A Danks, T John Martin, Atsuhiko Hattori, Masaki Nakano, Makiko Kakikawa, Sotoshi Yamada, Maho Ogoshi, Susumu Hyodo, Yoko Yamaguchi, Vishwajit S Chowdhury, Kazuichi Hayakawa, Hisayuki Funahashi, Tatsuya Sakamoto, Yuichi Sasayama

    Gene499 ( 2 ) 326 - 31   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin receptor (CTR) (46.1%), frog CTR (54.7%), and flounder CTR (48.9%). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5%, frog 50.7%, flounder 48.0%) and CTRs (human 43.2%, frog 49.1%, flounder 41.8%) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20% seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish.

    DOI: 10.1016/j.gene.2012.03.042

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  • Rapid signaling of steroid hormones in the vertebrate nervous system. 査読 国際誌

    Hirotaka Sakamoto, Hideya Takahashi, Ken-Ichi Matsuda, Mayumi Nishi, Keiko Takanami, Maho Ogoshi, Tatsuya Sakamoto, Mitsuhiro Kawata

    Frontiers in bioscience (Landmark edition)17   996 - 1019   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS IN BIOSCIENCE INC  

    Steroid hormones easily cross the blood-brain barrier because of their physicochemical lipid solubility. The hormones act through nuclear receptor-mediated mechanisms and modulate gene transcription. In contrast to their genomic actions, the non-genomic rapid action of steroid hormones, acting via various types of membrane-associated receptors, reveals pharmacological properties that are distinct from the actions of the intracellular nuclear receptors. As a result, non-genomic rapid actions have gained increased scientific interest. However, insight into the phylogenic and/or comparative actions of steroids in the brain is still poorly understood. In this review, we summarize recent findings concerning the rapid, non-genomic signaling of steroid hormones in the vertebrate central nervous system, and we discuss (using a comparative view from fish to mammals) recently published data regarding the mechanism underlying physiology and behavior.

    DOI: 10.2741/3970

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  • O2-filled swimbladder employs monocarboxylate transporters for the generation of O2 by lactate-induced root effect hemoglobin. 査読 国際誌

    Takahiro Umezawa, Akira Kato, Maho Ogoshi, Kayoko Ookata, Keijiro Munakata, Yoko Yamamoto, Zinia Islam, Hiroyuki Doi, Michael F Romero, Shigehisa Hirose

    PloS one7 ( 4 ) e34579   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    The swimbladder volume is regulated by O(2) transfer between the luminal space and the blood In the swimbladder, lactic acid generation by anaerobic glycolysis in the gas gland epithelial cells and its recycling through the rete mirabile bundles of countercurrent capillaries are essential for local blood acidification and oxygen liberation from hemoglobin by the "Root effect." While O(2) generation is critical for fish flotation, the molecular mechanism of the secretion and recycling of lactic acid in this critical process is not clear. To clarify molecules that are involved in the blood acidification and visualize the route of lactic acid movement, we analyzed the expression of 17 members of the H(+)/monocarboxylate transporter (MCT) family in the fugu genome and found that only MCT1b and MCT4b are highly expressed in the fugu swimbladder. Electrophysiological analyses demonstrated that MCT1b is a high-affinity lactate transporter whereas MCT4b is a low-affinity/high-conductance lactate transporter. Immunohistochemistry demonstrated that (i) MCT4b expresses in gas gland cells together with the glycolytic enzyme GAPDH at high level and mediate lactic acid secretion by gas gland cells, and (ii) MCT1b expresses in arterial, but not venous, capillary endothelial cells in rete mirabile and mediates recycling of lactic acid in the rete mirabile by solute-specific transcellular transport. These results clarified the mechanism of the blood acidification in the swimbladder by spatially organized two lactic acid transporters MCT4b and MCT1b.

    DOI: 10.1371/journal.pone.0034579

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  • Hemolymph osmotic, ionic status, and branchial Na+/K+-ATPase activity under varying environmental conditions in the intertidal grapsid crab, Gaetice depressusd.

    T. Nanba, H. Takahashi, T. Abe, W. Godo, M. Ogoshi, H. Sakamoto, N. Tsutsui, T. Sakamoto

    International Aquatic Research. 4 (2012) 1-12.   2012年

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  • Corticosteroids stimulate the amphibious behavior in mudskipper: potential role of mineralocorticoid receptors in teleost fish. 査読 国際誌

    Tatsuya Sakamoto, Chie Mori, Shogo Minami, Hideya Takahashi, Tsukasa Abe, Daisuke Ojima, Maho Ogoshi, Hirotaka Sakamoto

    Physiology & behavior104 ( 5 ) 923 - 8   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    It has long been held that cortisol, a glucocorticoid in many vertebrates, carries out both glucocorticoid and mineralocorticoid actions in teleost fish. However, 11-deoxycorticosterone (DOC) has been identified as a specific endogenous ligand for the teleostean mineralocorticoid receptor (MR). Furthermore, the expressions of MR mRNA are modest in the osmoregulatory organs, but considerably higher in the brain of most teleosts. These recent findings suggest that the mineralocorticoid system (DOC/MR) may carry out some behavioral functions in fish. To test this possibility, we examined the effects of cortisol and DOC administration in the amphibious behavior in mudskipper (Periophthalmus modestus) in vivo. It was found that mudskippers remained in the water for an increased period of time when they were immersed into 5 μM DOC or cortisol for 8h. Additionally, an exposure to 25 μM DOC for 4 to 8 h caused a decreased migratory frequency of mudskippers to the water, reflected a tendency to remain in the water. It was further observed that after 8 h of intracerebroventricular (ICV) injection with 0.3 pmol DOC or cortisol the staying period in the water increased in fish. The migratory frequency was decreased after ICV DOC injection which indicated that fishes stayed in the water. Concurrent ICV injections of cortisol with RU486 [a specific glucocorticoid-receptor (GR) antagonist] inhibited only the partial effects of cortisol. Together with no changes in the plasma DOC concentrations under terrestrial conditions, these results indicate the involvement of brain MRs as cortisol receptors in the preference for an aquatic habitat of mudskippers. Although the role of GR signaling cannot be excluded in the aquatic preference, our data further suggest that the MR may play an important role in the brain dependent behaviors of teleost fish.

    DOI: 10.1016/j.physbeh.2011.06.002

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  • Differential expression of Na+-Cl- cotransporter and Na+-K+-Cl- cotransporter 2 in the distal nephrons of euryhaline and seawater pufferfishes. 査読 国際誌

    Akira Kato, Takayuki Muro, Yuuri Kimura, Shanshan Li, Zinia Islam, Maho Ogoshi, Hiroyuki Doi, Shigehisa Hirose

    American journal of physiology. Regulatory, integrative and comparative physiology300 ( 2 ) R284-97 - R297   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    The process of NaCl reabsorption in the distal nephron allows freshwater fishes to excrete hypotonic urine and seawater fishes to excrete urine containing high concentrations of divalent ions; the relevant transporters, however, have not yet been identified. In the mammalian distal nephron, NaCl absorption is mediated by Na(+)-K(+)-Cl(-) cotransporter 2 (NKCC2, Slc12a1) in the thick ascending limb, Na(+)-Cl(-) cotransporter (NCC, Slc12a3) in the distal convoluted tubule, and epithelial sodium channel (ENaC) in the collecting duct. In this study, we compared the expression profiles of these proteins in the kidneys of euryhaline and seawater pufferfishes. Mining the fugu genome identified one NKCC2 gene and one NCC gene, but no ENaC gene. RT-PCR and in situ hybridization analyses demonstrated that NKCC2 was highly expressed in the distal tubules and NCC was highly expressed in the collecting ducts of euryhaline pufferfish (mefugu, Takifugu obscurus). On the other hand, the kidney of seawater pufferfish (torafugu, Takifugu rubripes), which lacked distal tubules, expressed very low levels of NCC, and, in the collecting ducts, high levels of NKCC2. Acclimation of mefugu to seawater resulted in a 2.7× decrease in NCC expression, whereas NKCC2 expression was not markedly affected. Additionally, internalization of NCC from the apical surface of the collecting ducts was observed. These results suggest that NaCl reabsorption in the distal nephron of the fish kidney is mediated by NCC and NKCC2 in freshwater and by NKCC2 in seawater.

    DOI: 10.1152/ajpregu.00725.2009

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  • Biological functions of adrenomedullin family in teleost fish 査読

    Ogoshi Maho, Nobata Shigenori, Inoue Koji, Nag Kakon, Takei Yoshio, Hirose Shigehisa

    ENDOCRINE JOURNAL57   S445   2010年3月

  • Molecular and functional evolution of the adrenomedullin family in vertebrates: What do fish studies tell us?

    Yoshio Takei, Maho Ogoshi, Marty, K.S. Wong, Shigenori Nobata

    The Calcitonin Gene-related Peptide Family: Form, Function and Future Perspectives   1 - 21   2010年1月

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    © Springer Science+Business Media B.V. 2010. All rights reserved. Adrenomedullin (AM) comprises a unique family of five paralogous peptides (AM1, 2, 3, 4 and 5) in teleost fish, of which AM1 is an ortholog of mammalian AM, and AM1/4 and AM2/3 were produced at the teleost-specific whole genome duplication. Therefore, CGRP, amylin, AM1, AM2 and AM5 existed when ray-finned fish and lobe-finned fish (leading to tetrapods) were diverged. Based on this finding, we discovered novel AM2 and AM5 in mammals. In addition, comparative genomic analyses based on fish studies delineated an evolutionary history of the CGRP family of peptides in vertebrates. As a first chapter of this volume, we initially propose an idea of how the CGRP family, including multiple AM peptides, have been organized during the course of vertebrate evolution. We will also show how comparative fish studies can contribute to general and clinical endocrinology by providing new insights into the molecule and function of the CGRP family throughout vertebrate species.

    DOI: 10.1007/978-90-481-2909-6_1

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  • Identification of renal transporters involved in sulfate excretion in marine teleost fish. 査読 国際誌

    Akira Kato, Min-Hwang Chang, Yukihiro Kurita, Tsutomu Nakada, Maho Ogoshi, Takeru Nakazato, Hiroyuki Doi, Shigehisa Hirose, Michael F Romero

    American journal of physiology. Regulatory, integrative and comparative physiology297 ( 6 ) R1647-59 - R1659   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Sulfate (SO(4)(2-)) is the second most abundant anion in seawater (SW), and excretion of excess SO(4)(2-) from ingested SW is essential for marine fish to survive. Marine teleosts excrete SO(4)(2-) via the urine produced in the kidney. The SO(4)(2-) transporter that secretes and concentrates SO(4)(2-) in the urine has not previously been identified. Here, we have identified and characterized candidates for the long-sought transporters. Using sequences from the fugu database, we have cloned cDNA fragments of all transporters belonging to the Slc13 and Slc26 families from mefugu (Takifugu obscurus). We compared Slc13 and Slc26 mRNA expression in the kidney between freshwater (FW) and SW mefugu. Among 14 clones examined, the expression of a Slc26a6 paralog (mfSlc26a6A) was the most upregulated (30-fold) in the kidney of SW mefugu. Electrophysiological analyses of Xenopus oocytes expressing mfSlc26a6A, mfSlc26a6B, and mouse Slc26a6 (mSlc26a6) demonstrated that all transporters mediate electrogenic Cl(-)/SO(4)(2-), Cl(-)/oxalate(2-), and Cl(-)/nHCO(3)(-) exchanges and electroneutral Cl(-)/formate(-) exchange. Two-electrode voltage-clamp experiments demonstrated that the SO(4)(2-)-elicited currents of mfSlc26a6A is quite large (approximately 35 microA at +60 mV) and 50- to 200-fold higher than those of mfSlc26a6B and mSlc26a6. Conversely, the currents elicited by oxalate and HCO(3)(-) are almost identical among mfSlc26a6A, mfSlc26a6B, and mSlc26a6. Kinetic analysis revealed that mfSlc26a6A has the highest SO(4)(2-) affinity as well as capacity. Immunohistochemical analyses demonstrated that mfSlc26a6A localizes to the apical (brush-border) region of the proximal tubules. Together, these findings suggest that mfSlc26a6A is the most likely candidate for the major apical SO(4)(2-) transporter that mediates SO(4)(2-) secretion in the kidney of marine teleosts.

    DOI: 10.1152/ajpregu.00228.2009

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  • Potent osmoregulatory actions of homologous adrenomedullins administered peripherally and centrally in eels. 査読 国際誌

    Maho Ogoshi, Shigenori Nobata, Yoshio Takei

    American journal of physiology. Regulatory, integrative and comparative physiology295 ( 6 ) R2075-83 - R2083   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    The teleost adrenomedullin (AM) family consists of three groups, AM1/AM4, AM2/AM3, and AM5. In the present study, we examined the effects of homologous AM1, AM2, and AM5 on drinking and renal function after peripheral or central administration in conscious freshwater eels. AM2 and AM5, but not AM1, exhibited dose-dependent (0.01-1 nmol/kg) dipsogenic and antidiuretic effects after intra-arterial bolus injection. The antidiuretic effect was significantly correlated with the degree of associated hypotension. To avoid the potential indirect osmoregulatory effects of AM-induced hypotension, infusion of AMs was also performed at nondepressor doses. Drinking was enhanced dose-dependently at 0.1-3 pmol.kg(-1).min(-1) of AM2 and AM5, matching the potency and efficacy of angiotensin II (ANG II), the most potent dipsogenic hormone known thus far. AM2 and AM5 infusion also induced mild antidiuresis, while AM1 caused antinatriuresis. Additionally, AMs were injected into the third and fourth ventricles of conscious eels to assess their site of dipsogenic action. However, none of the AMs at 0.05-0.5 nmol induced drinking, while ANG II was highly dipsogenic. AM2 and ANG II injected into the third ventricle increased arterial pressure while AM5 decreased it in a dose-dependent manner, and both AM2 and AM5 decreased blood pressure when injected into the fourth ventricle. These data suggest that circulating AM2 and AM5 act on a target site in the brain that lacks the blood-brain barrier. Collectively, the present study showed that AM2 and AM5 are potent osmoregulatory hormones in the eel, and their actions imply involvement in seawater adaptation of this euryhaline species.

    DOI: 10.1152/ajpregu.90688.2008

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  • Central and peripheral cardiovascular actions of adrenomedullin 5, a novel member of the calcitonin gene-related peptide family, in mammals

    Y. Takei, H. Hashimoto, K. Inoue, T. Osaki, K. Yoshizawa-Kumagaye, M. Tsunemi, T. X. Watanabe, M. Ogoshi, N. Minamino, Y. Ueta

    Journal of Endocrinology197   391 - 400   2008年5月

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    Adrenomedullin 5 (AM5) is a new member of the calcitonin gene-related peptide (CGRP) family idetitified in teleost fish. Although its presence was suggested in the genome database of mammals, molecular identity and biological function of AM5 have not been examined yet. In this study, we cloned a cDNA encoding AM5 in the pig and examined its cardiovascular and renal effects. Putative mature AM5 was localized in the middle of prohormone and had potential signals for intermolecular ring formation and C-terminal amidation. The AM5 gene was expressed most abundantly in the spleen and thymus. Several AM5 genes were newly identified in the database of mammals, which revealed that the AM5 gene exists in primates, carnivores, and undulates but could not be identified in rodents. In primates, nucleotide deletion occurred in the mature AM5 sequence in anthropoids (human and chimp) during transition from the rhesus monkey. Synthetic mature AM5 injected intravenously into rats induced dose-dependent decreases in arterial pressure at 0.1-1 nmol/kg without apparent changes in heart rate. The decrease was maximal in 1 min and AM5 was approximately half as potent as AM. AM5 did not cause significant changes in urine flow and urine Na+ concentration at any dose. In contrast to the peripheral vasodepressor action, AM5 injected into the cerebral ventricle dose-dependently increased arterial pressure and heart rate at 0.1-1 nmol. The increase reached maximum more quickly after AM5 (5 min) than AM (15-20 min). AM5 added to the culture cells expressing calcitonin receptor-like receptor (CLR) or calcitonin receptor (CTR) together with one of the receptor activity-modifying proteins (RAMPs), the combination of which forms major receptors for the CGRP family, did not induce appreciable increases in cAMP production in any combination, although AM increased it at 1010 -10-9 M when added to the CLR and RAMP2/3 combination. These data indicate that AM5 seems to act on as yet unknown receptor(s) for AM5, other than CLP/CTR+RAMP, to exert central and peripheral cardiovascular actions in mammals. © 2008 Society for Endocrinology.

    DOI: 10.1677/JOE-07-0541

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  • Potent cardiovascular actions of homologous adrenomedullins in eels. 査読 国際誌

    Shigenori Nobata, Maho Ogoshi, Yoshio Takei

    American journal of physiology. Regulatory, integrative and comparative physiology294 ( 5 ) R1544-53 - R1553   2008年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Adrenomedullin (AM), known as a multifunctional hormone in mammals, forms a unique family of five paralogous peptides in teleost fish. To examine their cardiovascular effects using homologous AMs in eels, we isolated cDNAs encoding four eel AMs, and named AM1 (ortholog of mammalian AM), AM2, AM3 (paralog of AM2 generated only in teleost lineage), and AM5 according to the known teleost AM sequences. Unlike pufferfish, not only AM1 but AM2/3 and AM5 were expressed ubiquitously in various eel tissues. Synthetic mature AM1, AM2, and AM5 exhibited vasodepressor effects after intra-arterial injections, and the effects were more potent at dorsal aorta than at ventral aorta. This indicates that AMs preferentially act on peripheral resistance vessels rather than on branchial arterioles. The potency was in the order of AM2 = AM5 > AM1 in both freshwater (FW) and seawater (SW) eels, which is different from the result of mammals in which AM1 is as potent as, or more potent than, AM2 when injected peripherally. The minimum effective dose of AM2 and AM5 in eels was 1/10 that of AM1 in mammals. The hypotension reached 50% at 1.0 nmol/kg of AM2 and AM5, which is much greater than atrial natriuretic peptide (20%), another potent vasodepressor hormone. Even with such hypotension, AMs did not change heart rate in eels. In addition, AM1 increased blood pressure at ventral aorta and dorsal aorta immediately after an initial hypotension at 5.0 nmol/kg, but not with AM2 and AM5. These data strongly suggest that specific receptors for AM2 and AM5 exist in eels, which differ from the AM1 receptors identified in mammals.

    DOI: 10.1152/ajpregu.00707.2007

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  • A 'reverse' phylogenetic approach for identification of novel osmoregulatory and cardiovascular hormones in vertebrates. 査読 国際誌

    Yoshio Takei, Maho Ogoshi, Koji Inoue

    Frontiers in neuroendocrinology28 ( 4 ) 143 - 60   2007年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Vertebrates expanded their habitats from aquatic to terrestrial environments during the course of evolution. In parallel, osmoregulatory and cardiovascular systems evolved to counter the problems of desiccation and gravity on land. In our physiological studies on body fluid and blood pressure regulation in various vertebrate species, we found that osmoregulatory and cardiovascular hormones have changed their structure and function during the transition from aquatic to terrestrial life. In fact, Na(+)-regulating and vasodepressor hormones play essential roles in fishes, while water-regulating and vasopressor hormones are dominant in tetrapods. Accordingly, Na(+)-regulating and vasodepressor hormones, such as natriuretic peptide (NP) and adrenomedullin (AM), are much diversified in teleost fishes compared with mammals. Based on this finding, new NPs and AMs were identified in mammals and other tetrapods. These hormones have only minor roles in the maintenance of normal blood volume and pressure in mammals, but their importance seems to increase when homeostasis is disrupted. Therefore, such hormones can be used for diagnosis and treatment of body fluid and cardiovascular disorders such as cardiac/renal failure and hypertension. In this review, we introduce a new approach for identification of novel Na(+)-regulating and vasodepressor hormones in mammals based on fish studies. Until recently, new hormones were first discovered in mammals, and then identified and applied in fishes. However, chances are increasing in recent years to identify new hormones first in fishes then in mammals, based on the difference in the regulatory systems between fishes and tetrapods. As the direction is opposite from the traditional phylogenetic approach, we added 'reverse' to its name. The 'reverse' phylogenetic approach offers a typical example of how comparative fish studies can contribute to the general and clinical endocrinology.

    DOI: 10.1016/j.yfrne.2007.05.001

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  • Fish calcitonin receptor has novel features 査読

    Kakon Nag, Akira Kato, Naznin Sultana, Maho Ogoshi, Yoshio Takei, Shigehisa Hirose

    GENERAL AND COMPARATIVE ENDOCRINOLOGY154 ( 1-3 ) 48 - 58   2007年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Calcitonin (CT), a 32-amino acid peptide, was initially isolated from fish. Fish CT has higher affinity to mammalian CT receptor (CTR). and has activity on calcium homeostasis. Therefore, fish CT has been used as a drug for the treatment of human bone diseases. However, the physiological roles of CT in fish as well as the characteristics of the fish CTR have not been clarified. Here, we cloned and characterized CTR from mefugu (Takfiigu obscurus). Full-length cDNA sequencing revealed that mfCTR (mf, mefugu) consists of N-terminal four tandem putative hormone-binding domains (HBDs). Database mining showed that the multiple HBD-containing CTR is a common feature for some other fishes. Detailed pharmacological studies revealed that mfCTR generated cAMP in response to (1) fish CT (2) calcitonin gene-related peptide (CGRP) in combinations with receptor activity-modifying proteins (mfRAMPs) I and 4, and (3) amylin in combinations with mfRAMPs 1-5. Unlike mammalian CTR. mfCTR showed dual affinity sites. Corresponding EC50 values of those are in close proximity of the in vivo concentration of CT in fish. Analyses of the deletion mutants of mfCTR demonstrated that only the nearmost HBD to the first transmembrane region is functional to the ligands. Although, fish CT has higher affinity to the human CTR. human CT did not bind to the mfCTR. This is the first report that demonstrates the structure and property of fish receptor for CT. CGRR and amylin. Fish CTR is the first example that has multiple HBD-like sequences. (C) 2007 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ygcen.2007.06.017

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  • Evolutionary history of the calcitonin gene-related peptide family in vertebrates revealed by comparative genomic analyses. 査読 国際誌

    Maho Ogoshi, Koji Inoue, Kiyoshi Naruse, Yoshio Takei

    Peptides27 ( 12 ) 3154 - 64   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    The calcitonin gene-related peptide (CGRP) family is composed of CGRP, amylin and adrenomedullin (AM) in mammals. In teleost fish, AM forms an independent subfamily of five members (AM1-5), which inspired us to trace the evolutionary history of the CGRP family throughout vertebrates by comparative genomic approach. Linkage mapping and synteny analyses of the CGRP family genes in medaka, Oryzias latipes, revealed that AM1/CGRP, AM2/amylin, and AM5 genes were located on respective proto-chromosomes before the divergence of teleost lineage. In teleost fish, additional whole genome duplication generated AM1/4, CGRP1/2, AM2/3, but one of the duplicated amylin and AM5 genes was silenced. In mammals, the amylin or AM2 gene was translocated to different chromosomes, while the CGRP gene was multiplied in tandem to generate CGRP-alpha,beta, and recently identified calcitonin receptor-stimulating peptide genes. Based on these data, we identified a novel AM5 gene in several mammalian species as we previously did for AM2.

    DOI: 10.1016/j.peptides.2006.09.011

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  • Evolutionary history of the CGRP family in vertebrates revealed by comparative genomic analyses 査読

    Ogoshi Maho, Inoue Koji, Naruse Kiyoshi, Takei Yoshio

    ZOOLOGICAL SCIENCE23 ( 12 ) 1210   2006年12月

  • Contribution of comparative fish studies to general endocrinology: structure and function of some osmoregulatory hormones. 査読 国際誌

    Yoshio Takei, Akatsuki Kawakoshi, Takehiro Tsukada, Shinya Yuge, Maho Ogoshi, Koji Inoue, Susumu Hyodo, Hideo Bannai, Satoru Miyano

    Journal of experimental zoology. Part A, Comparative experimental biology305 ( 9 ) 787 - 98   2006年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Fish endocrinologists are commonly motivated to pursue their research driven by their own interests in these aquatic animals. However, the data obtained in fish studies not only satisfy their own interests but often contribute more generally to the studies of other vertebrates, including mammals. The life of fishes is characterized by the aquatic habitat, which demands many physiological adjustments distinct from the terrestrial life. Among them, body fluid regulation is of particular importance as the body fluids are exposed to media of varying salinities only across the thin respiratory epithelia of the gills. Endocrine systems play pivotal roles in the homeostatic control of body fluid balance. Judging from the habitat-dependent control mechanisms, some osmoregulatory hormones of fish should have undergone functional and molecular evolution during the ecological transition to the terrestrial life. In fact, water-regulating hormones such as vasopressin are essential for survival on the land, whereas ion-regulating hormones such as natriuretic peptides, guanylins and adrenomedullins are diversified and exhibit more critical functions in aquatic species. In this short review, we introduce some examples illustrating how comparative fish studies contribute to general endocrinology by taking advantage of such differences between fishes and tetrapods. In a functional context, fish studies often afford a deeper understanding of the essential actions of a hormone across vertebrate taxa. Using the natriuretic peptide family as an example, we suggest that more functional studies on fishes will bring similar rewards of understanding. At the molecular level, recent establishment of genome databases in fishes and mammals brings clues to the evolutionary history of hormone molecules via a comparative genomic approach. Because of the functional and molecular diversification of ion-regulating hormones in fishes, this approach sometimes leads to the discovery of new hormones in tetrapods as exemplified by adrenomedullin 2.

    DOI: 10.1002/jez.a.309

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  • Contribution of comparative fish studies to general endocrinology: structure and function of some osmoregulatory hormones 査読

    Takei Yoshio, Kawakoshi Akatsuki, Tsukada Takehiro, Yuge Shinya, Ogoshi Maho, Inoue Koji, Hyodo Susumu, Bannai Hideo, Miyano Satoru

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY305A ( 9 ) 787 - 798   2006年9月

  • Identification of novel adrenomedullin in mammals: a potent cardiovascular and renal regulator. 査読 国際誌

    Yoshio Takei, Koji Inoue, Maho Ogoshi, Tetsushi Kawahara, Hideo Bannai, Satoru Miyano

    FEBS letters556 ( 1-3 ) 53 - 8   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We have identified cDNA encoding a new member of the adrenomedullin (AM) family, AM2, for the first time in mammals (mouse, rat and human). The predicted precursor carried mature AM2 in the C-terminus, which had an intramolecular ring formed by an S-S bond and a possibly amidated C-terminus. Phylogenetic analyses clustered AM2 and AM into two distinct but closely related groups. Similarity of exon-intron structure and synteny of neighboring genes showed that mammalian AM2 is an ortholog of pufferfish AM2 and a paralog of mammalian AM. AM2 mRNA was expressed in submaxillary gland, kidney, stomach, ovary, lymphoid tissues and pancreas of mice, but not in adrenal and testis. Intravenous injection of synthetic mature AM2 decreased arterial pressure more potently than AM, and induced antidiuresis and antinatriuresis in mice. These results show that at least two peptides, AM and AM2, comprise an adrenomedullin family in mammals, and that AM2 may play pivotal roles in cardiovascular and body fluid regulation.

    DOI: 10.1016/S0014-5793(03)01368-1

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  • Identification of a novel adrenomedullin gene family in teleost fish. 査読 国際誌

    Maho Ogoshi, Koji Inoue, Yoshio Takei

    Biochemical and biophysical research communications311 ( 4 ) 1072 - 7   2003年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Adrenomedullin (AM) is a multifunctional peptide known to form a hormone family with calcitonin gene-related peptide (CGRP) and amylin. We have cloned five distinct AM cDNAs from the pufferfish, Takifugu rubripes, and named them TrAM-1, -2, -3, -4, and -5. Judging from the deduced precursor sequences and processing pattern of the C-terminal mature peptides, TrAMs may be divided into at least two groups; AM-2 and -3, and AM-1, -4, and possibly -5. Phylogenetic analysis of the mature peptides, exon-intron structure of their genes, and tissue distribution of their mRNA also support this classification. TrAM-1 and -4 were ubiquitously expressed in various tissues including the kidney and interrenal (adrenal homolog) as in the case of mammalian AM, while TrAM-2 and -3 were expressed most abundantly in the brain followed by the vascular tissues. Synteny of the genes around AM gene showed that TrAM-1 is the ortholog of mammalian AM. The presence of a PAMP-like sequence in the prosegment of TrAM-1 also supports this notion. Multiple AMs were also detected in another pufferfish, Tetraodon nigroviridis, and in zebrafish, Danio rerio. The present study shows for the first time the presence of a novel AM family in teleost fish that is independent from CGRP and amylin, which further suggests the possible existence of multiple AMs in mammals.

    DOI: 10.1016/j.bbrc.2003.10.111

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  • ホメオスタシスと適応 : 恒

    海谷, 啓之, 内山, 実( 担当: 共著 ,  範囲: 水・電解質代謝とホルモン)

    裳華房  2016年8月  ( ISBN:9784785351182

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    総ページ数:xiv, 227p, 図版 [4] p   記述言語:日本語

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  • 基礎生物科学

    高橋, 純夫, 鷲谷, いづみ( 担当: 共著 ,  範囲: 循環と呼吸、浸透圧調節と腎臓)

    培風館  2016年7月  ( ISBN:9784563078218

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    総ページ数:vi, 238p   記述言語:日本語

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  • Handbook of hormones : comparative endocrinology for basic and clinical research

    竹井, 祥郎, 安東, 宏徳, 筒井, 和義( 担当: 共著 ,  範囲: Calcitonin Gene-Related Peptide; Calcitonin Receptor-Stimulating Peptide; Adrenomedullin; Adrenomedullin 2 and 5; Amylin)

    Academic Press is an imprint of Elsevier  2016年  ( ISBN:9780128010280

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    総ページ数:xxvii, 646 p.   記述言語:英語

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  • Eel physiology

    Trischitta, Francesca, 竹井, 祥郎, Sébert, Philippe( 担当: 共著 ,  範囲: Endocrine control of osmoregulation)

    CRC Press  2014年  ( ISBN:9781466598270

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    総ページ数:viii, 370 p.   記述言語:英語

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  • 現代生物学入門

    岡山大学生物学教科書作成委員会, 高橋, 純夫, 阿保, 達彦( 担当: 分担執筆 ,  範囲: 浸透圧応答)

    岡山大学出版会  2011年3月  ( ISBN:9784904228197

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    総ページ数:185p   記述言語:日本語

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  • The Calcitonin Gene-Related Peptide Family: Form, Function and Future Perspectives

    Hay DL, Dickerson I( 担当: 共著 ,  範囲: Molecular and functional evolution of the adrenomedullin family in vertebrates: What do fish tell us?)

    Springer Verlag  2009年 

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MISC

  • ツメガエル属におけるAdrenomedullin 5の局在と免疫に関する機能

    御輿真穂, 御輿真穂, 山浦晋平, 青柳康太, 荒木文平, 相澤清香, 相澤清香, 竹内栄, 竹内栄, 高橋純夫, 高橋純夫

    岡山実験動物研究会報 ( 35 )   2019年

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  • ラット隆起部におけるニューロメジンUの発現とその制御メカニズム

    相澤清香, 顧ていてい, 神之田有紗, 藤岡竜矢, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報 ( 34 )   2018年

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  • ニワトリにおける遅羽性K遺伝子の作用メカニズムの解析

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    岡山実験動物研究会報 ( 34 )   2018年

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  • 下垂体隆起部の発生期に特異的に発現する遺伝子Cytokine-like 1,Gap junction protein alpha 5の同定

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  • 海洋生物の適応戦略-新規技術・現象からの新展開-水棲脊椎動物におけるアドレノメデュリンファミリーの新たな特徴

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    月刊海洋49 ( 5 )   2017年

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  • マウス子宮内膜におけるTGF-β遺伝子の発現制御

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  • ラット脳下垂体隆起部の新規生理機能の探索

    相澤清香, 坂井田初季, 坂田一郎, 坂井貴文, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報 ( 33 )   2017年

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  • ウズラPBCF遺伝子の構造と発現

    釘本綾子, 相澤清香, 御輿真穂, 高橋純夫, 竹内栄

    岡山実験動物研究会報32 ( 32 ) 19 - 21   2016年

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  • マウス子宮内膜細胞におけるKallikreinの発現制御の解析

    徳森萌美, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報31 ( 31 ) 37 - 39   2015年

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  • マウス卵巣機能制御における転写因子Runx3の生理的役割

    小島史也, 斉藤優佳, 土家由紀子, 御輿真穂, 御輿真穂, 竹内栄, 竹内栄, 高橋純夫, 高橋純夫

    岡山実験動物研究会報 ( 31 )   2015年

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  • ニワトリにおける羽色調節の品種差

    高橋徹, 西尾香織, 御輿真穂, 高橋純夫, 竹内栄

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  • マウス卵巣顆粒膜細胞におけるインスリン様成長因子I遺伝子の発現

    林紗代, 小島史也, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報 ( 31 )   2015年

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  • Runx3 Regulates Ovulation and Steroidogenesis in Granulosa Cells in Mice

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    ENDOCRINE REVIEWS35 ( 3 )   2014年6月

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  • ニワトリにおける羽色調節の品種差

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  • The mechanism of local blood acidification in the swimbladder by spatially organized monocarboxylate transporters

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  • 第36回日本比較内分泌学会大会<br>若手交流会パネルディスカッション総括

    御輿 真穂, 日下部 誠, 今野 紀文

    比較内分泌学38 ( 144 ) 14 - 16   2012年

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  • 脊椎動物におけるアドレノメデュリンファミリーの進化と機能

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    比較内分泌学37 ( 141 )   2011年

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  • 鰾(うきぶくろ)奇網に高発現する乳酸輸送体(monocarboxylate transporters MCT1b and MCT4b)の同定と血液酸性化およびO<sub>2</sub>分泌における役割

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    生化学   2010年

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  • Fishing novel hormone genes from fish database

    Y Takei, M Ogoshi, A Kawakoshi, K Inoue, S Hyodo

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY305A ( 2 ) 183 - 183   2006年2月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:WILEY-LISS  

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  • カルシトニン遺伝子関連ペプチド(CGRP)ファミリーの分子進化

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    生体の科学57 ( 5 ) 436 - 438   2006年

  • アドレノメデュリン研究の新展開 比較内分泌学からの発信:新規アドレノメデュリン(AM2)の発見

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    分子心血管病6 ( 5 )   2005年

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  • A novel adrenomedullin family identified in fish

    Maho Ogoshi, Yoshio Takei

    ZOOLOGICAL SCIENCE21 ( 12 ) 1230 - 1230   2004年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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  • 魚類のadrenomedullin受容体ファミリーの単離と機能解析

    KAKON N, 加藤明, 御輿真穂, 竹井祥郎, 広瀬茂久

    日本内分泌学会雑誌80 ( 2 )   2004年

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▼全件表示

 

担当授業科目

  • 教養生物学(動植物) (2020年度) 第1学期  - 金1,金2

  • 比較内分泌学 (2020年度) 後期  - 木1,木2

  • 細胞生物学II (2020年度) 1・2学期  - 水1,水2

  • 細胞生物学IIB (2020年度) 第2学期  - 水1,水2