Updated on 2025/06/06

写真a

 
OGOSHI Maho
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Associate Professor
Position
Associate Professor
Homepage
https://kaken.nii.ac.jp/d/r/00527997.ja.html
External link

Degree

  • 博士（理学） ( 2008.3   東京大学 )

  • 博士（理学）

Research Interests

  • イソトシン

  • トビハゼ

  • 脳

  • 国際研究者交流

  • 環境適応

  • 神経行動

  • バソトシン

  • アドレノメデュリン

  • 脊椎動物

  • 比較内分泌学

Research Areas

  • Life Science / Evolutionary biology

  • Life Science / Morphology and anatomical structure  / 比較内分泌

Research History

  • Okayama University   学術研究院環境生命自然科学学域   Associate Professor

    2024.7

    　 More details

  • Okayama University   学術研究院自然科学学域   Assistant Professor

    2021.4 - 2024.6

    　 More details

  • Okayama University   The Graduate School of Natural Science and Technology   Assistant Professor

    2013.4 - 2021.3

    　 More details

  • Okayama University   Marine Laboratory

    2010.4 - 2013.3

    　 More details

  • Tokyo Institute of Technology   School of Bioscience and Biotechnology

    2009.4 - 2010.3

    　 More details

  • Tokyo Institute of Technology   School of Bioscience and Biotechnology

    2008.4 - 2009.3

    　 More details

  • The University of Tokyo   海洋研究所

    2005.4 - 2008.3

    　 More details

▼display all

 

Papers

  • Identification of pennaceous barbule cell factor (PBCF), a novel gene with spatiotemporal expression in barbule cells during feather development

    Minori Nakaoka, Hibiki Fukuchi, Maho Ogoshi, Sayaka Aizawa, Sakae Takeuchi

    Gene   941   149244 - 149244   2025.3

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.gene.2025.149244

    researchmap

  • Unilateral Chasing and Courtship-Like Display as Dominance Signals for Hierarchy Maintenance in Male Medaka Fish

    Yuji Mitsutsuji, Mayuko Suzuki, Ryo Umeda, Satoshi Ansai, Fumitaka Ueoka, Zou Jingyi, Anna Yamaguchi, Towako Hiraki-Kajiyama, Tetsuro Takeuchi, Maho Ogoshi, Takafumi Katsumura, Saori Yokoi, Hideaki Takeuchi

    Research Square   2024.9

    　More details

    Language：English  

    DOI： 10.21203/rs.3.rs-4837620/v1

    researchmap

  • Revisiting the hormonal control of sexual dimorphism in chicken feathers. Reviewed International journal

    Li You, Kaori Nishio, Kinue Kowata, Minaru Horikawa, Hibiki Fukuchi, Maho Ogoshi, Sayaka Aizawa, Sakae Takeuchi

    General and comparative endocrinology   114601 - 114601   2024.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Sexual dimorphism in plumage is widespread among avian species. In chickens, adult females exhibit countershading, characterized by dull-colored round feathers lacking fringe on the saddle, while adult males display vibrant plumage with deeply fringed bright feathers. This dimorphism is estrogen-dependent, and administering estrogen to males transforms their showy plumage into cryptic female-like plumage. Extensive studies have shown that estrogen's role in female plumage formation requires thyroid hormone; however, the precise mechanisms of their interaction remain unclear. In this study, we investigated the roles of estrogen and thyroid hormone in creating sexual dimorphism in the structure and coloration of saddle feathers by administering each hormone to adult males and observing the resulting changes in regenerated feathers induced by plucking. RT-PCR analysis revealed that the expression of type 3 deiodinase (DIO3), responsible for thyroid hormone inactivation, correlates with fringing. Estrogen suppressed DIO3 and agouti signaling protein (ASIP) expression while stimulating BlSK1, a marker of barbule cells, resulting in female-like feathers with mottled patterns and lacking fringes. Administration of thyroxine (T4) stimulated BlSK1 and proopiomelanocortin (POMC) expression, with no effect on ASIP, leading to the formation of solid black feathers lacking fringes. Triiodothyronine (T3) significantly increased POMC expression in pulp cells in culture. Taken together, these findings suggest that estrogen promotes the formation of solid vanes by suppressing DIO3 expression, while also inducing the formation of mottled patterns through inhibition of ASIP expression and indirect stimulation of melanocortin expression via changes in local T3 concentration. This is the first report describing molecular mechanism underlying hormonal crosstalk in creating sexual dimorphism in feathers.

    DOI： 10.1016/j.ygcen.2024.114601

    PubMed

    researchmap

  • A regulatory mechanism of mouse kallikrein 1 gene expression by estrogen Reviewed

    Takumi Iwasaki, Megumi Tokumori, Misaki Matsubara, Fumiya Ojima, Kana Kamigochi, Sayaka Aizawa, Maho Ogoshi, Atsushi P. Kimura, Sakae Takeuchi, Sumio Takahashi

    Molecular and Cellular Endocrinology   577   112044 - 112044   2023.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.mce.2023.112044

    researchmap

  • Gene duplication of C-type natriuretic peptide-4 (CNP4) in teleost lineage elicits subfunctionalization of ancestral CNP Reviewed

    Yukitoshi Katayama, Ami Saito, Maho Ogoshi, Yousuke Tsuneoka, Takao Mukuda, Morio Azuma, Makoto Kusakabe, Yoshio Takei, Takehiro Tsukada

    Cell and Tissue Research   388 ( 2 )   225 - 238   2022.2

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00441-022-03596-y

    researchmap

    Other Link： https://link.springer.com/article/10.1007/s00441-022-03596-y/fulltext.html

  • Adrenomedullin 2 and 5 activate the calcitonin receptor-like receptor (clr) - Receptor activity-modifying protein 3 (ramp3) receptor complex in Xenopus tropicalis. Reviewed International journal

    Maho Ogoshi, Mikoto Takahashi, Kota Aoyagi, Kazuyoshi Ukena, Sayaka Aizawa, Hideaki Takeuchi, Sumio Takahashi, Sakae Takeuchi

    General and comparative endocrinology   306   113752 - 113752   2021.5

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The adrenomedullin (AM) family is involved in diverse biological functions, including cardiovascular regulation and body fluid homeostasis, in multiple vertebrate lineages. The AM family consists of AM1, AM2, and AM5 in tetrapods, and the receptor for mammalian AMs has been identified as the complex of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 2 (RAMP2) or RAMP3. However, the receptors for AM in amphibians have not been identified. In this study, we identified the cDNAs encoding calcrl (clr), ramp2, and ramp3 receptor components from the western clawed frog (Xenopus tropicalis). Messenger RNAs of amphibian clr and ramp2 were highly expressed in the heart, whereas that of ramp3 was highly expressed in the whole blood. In HEK293T cells expressing clr-ramp2, cAMP response element luciferase (CRE-Luc) reporter activity was activated by am1. In HEK293T cells expressing clr-ramp3, CRE-Luc reporter activity was increased by the treatment with am2 at the lowest dose, but with am5 and am1 at higher dose. Our results provided new insights into the roles of AM family peptides through CLR-RAMP receptor complexes in the tetrapods.

    DOI： 10.1016/j.ygcen.2021.113752

    PubMed

    researchmap

  • Ghrelin and food acquisition in wild and cultured Japanese eel (Anguilla japonica). Reviewed International journal

    Takashi Yada, Michihisa Abe, Kenzo Kaifu, Kazuki Yokouchi, Nobuto Fukuda, Sakie Kodama, Hiroshi Hakoyama, Maho Ogoshi, Hiroyuki Kaiya, Tatsuya Sakamoto, Shunsuke Moriyama, Katsumi Tsukamoto

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology   245   110700 - 110700   2020.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    To clarify the relationships between growth, endocrine status and habitat characteristics in Japanese eel (Anguilla japonica), plasma and stomach mRNA levels of ghrelin were examined in wild eels captured in the river and the bay, and in cultured eels during and after experimental fasting. Wild juvenile eels captured in freshwater habitats within the river showed significantly higher plasma and stomach mRNA levels of ghrelin than did fish obtained from brackish-water habitats within the bay. In cultured eels experimentally fasted for 4 weeks, plasma and stomach mRNA levels of ghrelin increased. After refeeding, the both parameters returned to the levels observed in continuously feeding control fish. In pigmented elvers, 2 months of feed restriction resulted in a significant increase in whole-body ghrelin mRNA. It is suggested that interaction between ghrelin and feeding is related to their habitats through differential food acquisition in fresh and brackish water environments.

    DOI： 10.1016/j.cbpa.2020.110700

    PubMed

    researchmap

  • Adenosine stimulates neuromedin U mRNA expression in the rat pars tuberalis. Reviewed International journal

    Sayaka Aizawa, Tingting Gu, Arisa Kaminoda, Ryuya Fujioka, Fumiya Ojima, Ichiro Sakata, Takafumi Sakai, Maho Ogoshi, Sumio Takahashi, Sakae Takeuchi

    Molecular and cellular endocrinology   496 ( 496 )   110518 - 110518   2019.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Neuromedin U (NMU) shows circadian expression in the rat pars tuberalis (PT), and is known to be suppressed by melatonin. Here we examined the involvement of adenosine in the regulation of Nmu expression. We found that the rat PT expressed adenosine receptor A2b and that an adenosine receptor agonist, NECA, stimulated Nmu expression in brain slice cultures. In vitro promoter assays revealed that NECA stimulated Nmu promoter activity via a cAMP response element (CRE) in the presence of adenosine receptor A2b. NECA also increased the levels of phosphorylated CRE-binding protein. These findings suggest that adenosine stimulates Nmu expression by activating the cAMP signaling pathway through adenosine receptor A2b in the rat PT. This is the first report to demonstrate that Nmu expression in the PT is regulated by adenosine, which acts as an intravital central metabolic signal, in addition to melatonin, which acts as an external photoperiodic environmental signal.

    DOI： 10.1016/j.mce.2019.110518

    PubMed

    researchmap

  • Runx3 regulates folliculogenesis and steroidogenesis in granulosa cells of immature mice. Reviewed International journal

    Fumiya Ojima, Yuka Saito, Yukiko Tsuchiya, Maho Ogoshi, Hiroshi Fukamachi, Kenichi Inagaki, Fumio Otsuka, Sakae Takeuchi, Sumio Takahashi

    Cell and tissue research   375 ( 3 )   743 - 754   2019.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    We previously demonstrated that female Runx3 knockout (Runx3-/-) mice were anovulatory and their uteri were atrophic and that Runx3 mRNA was expressed in granulosa cells. To clarify how Runx3 regulates folliculogenesis and ovulation, we examine the effects of Runx3 knockout on the gene expression of growth factors associated with folliculogenesis and enzymes associated with steroidogenesis. In Runx3-/- mouse ovaries, the numbers of primary and antral follicles were lower than those in wild-type (wt) mice at 3 weeks of age, indicating that the loss of Runx3 affects folliculogenesis. The expression of genes encoding activin and inhibin subunits (Inha, Inhba and Inhbb) was also decreased in ovaries from the Runx3-/- mice compared with that in wt mice. Moreover, the expression of the genes Cyp11a1 and Cyp19a1 encoding steroidogenic enzymes was also decreased. In cultured granulosa cells from 3-week-old mouse ovaries, Cyp19a1 mRNA levels were lower in Runx3-/- mice than those in wt mice. Follicle-stimulating hormone (FSH) treatment increased Cyp19a1 mRNA levels in both wt and Runx3-/- granulosa cells in culture but the mRNA level in Runx3-/- granulosa cells was lower than that in wt ones, indicating that granulosa cells could not fully function in the absence of Runx3. At 3 weeks of age, gonadotropin α subunit, FSHβ subunit and luteinizing hormone (LH) β subunit mRNA levels were decreased in Runx3-/- mice. These findings suggest that Runx3 plays a key role in female reproduction by regulating folliculogenesis and steroidogenesis in granulosa cells.

    DOI： 10.1007/s00441-018-2947-2

    PubMed

    researchmap

  • Changes in prolactin receptor homodimer availability may cause late feathering in chickens. Reviewed International journal

    Ayako Okamura, Ayane Masumoto, Atsushi Takenouchi, Toshiyuki Kudo, Sayaka Aizawa, Maho Ogoshi, Sumio Takahashi, Masaoki Tsudzuki, Sakae Takeuchi

    General and comparative endocrinology   272   109 - 116   2019.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Chicken early (EF) and late feathering (LF) are sex-linked phenotypes conferred by wild-type k+ and dominant K alleles on chromosome Z, respectively. Besides prolactin (PRL) receptor (PRLR) and sperm flagellar 2 (SPEF2) genes, the K allele contains a fusion gene in which partially duplicated PRLR (dPRLR) and SPEF2 (dSPEF2) genes are linked in a tail-to-tail manner. The causative dPRLR gene encodes a C-terminal truncated receptor. LF chickens have short or no primaries at hatching; however, their feather growth rate is higher than that of EF chickens. This study aimed to elucidate the molecular basis of the K allele's biphasic effect on feather development. By 3'RACE and RT-PCR analyses, we demonstrated that dSPEF2 gene transcription occurred beyond all coding exons of the dPRLR gene on the opposite strand and that dPRLR mRNA was less abundant than PRLR mRNA. In addition, a 5'UTR splice variant (SPV) of PRL receptor mRNAs was increased in LF chickens. In vitro expression analysis of 5'UTR linked to the luciferase reporter gene revealed higher translation efficiency of SPV. RT-qPCR showed that the dPRLR mRNA level was higher in embryos; conversely, SPV was higher in hatched chickens, as was dSPEF2 mRNA. These findings suggest that the K allele inhibits feather development at the fetal stage by expressing dPRLR to attenuate PRLR function and promotes feather growth after hatching by increasing PRLR through dSPEF2 mRNA expression. Increased SPV may cause greater feather growth than that in EF chickens by increasing the availability of PRLR homodimers and enhancing PRL signaling.

    DOI： 10.1016/j.ygcen.2018.12.011

    PubMed

    researchmap

  • Runx3 transcription factor regulates ovarian functions and ovulation in female mice. Reviewed

    Fumiya Ojima, Yuka Saito, Yukiko Tsuchiya, Daichi Kayo, Syusuke Taniuchi, Maho Ogoshi, Hiroshi Fukamachi, Sakae Takeuchi, Sumio Takahashi

    The Journal of reproduction and development   62 ( 5 )   479 - 486   2016.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    We previously demonstrated that the Runx3 transcription factor is expressed in the hypothalami, pituitaries, and ovaries of mice, and that Runx3 knockout (Runx3-/-) mice are anovulatory and their uteri are atrophic. Runx3 mRNA expression was detected in the granulosa cells of ovarian follicles, and in the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC). In the present study, we examined the effects of Runx3 knockout on the gene expression of enzymes associated with steroidogenesis. We found decreased Cyp11a1 mRNA expression in Runx3-/- mouse ovaries compared with that in wild-type (wt) mouse ovaries at the age of 8 weeks. In situ hybridization analysis showed that the percentages of Cyp11a1 mRNA-expressing theca cells in follicles of Runx3-/- mice were decreased compared with those of wt mice. In accord with the alterations in Runx3-/- mouse ovaries, Kiss1 mRNA levels in ARC were increased, whereas mRNA levels of kisspeptin in AVPV were decreased, and gonadotropin-releasing hormone in the preoptic area and follicle-stimulating hormone β subunit gene were increased in Runx3-/- mice. Following an ovarian transplantation experiment between Runx3-/- mice and wt mice, corpora lutea were observed when ovaries from Runx3-/- mice were transplanted into wt mice, but not when those from wt mice were transplanted into Runx3-/- mice, suggesting that Runx3 in the hypothalamo-pituitary system may drive gonadotropin release to induce ovulation in the ovary. These findings indicate that Runx3 plays a crucial role in the hypothalamo-pituitary-gonadal axis.

    DOI： 10.1262/jrd.2016-005

    PubMed

    researchmap

  • Functional characterization of the mouse melanocortin 3 receptor gene promoter. Reviewed International journal

    Keisuke Okutsu, Fumiya Ojima, Naoto Shinohara, Shusuke Taniuchi, Yasusyo Mizote, Kenji Aoki, Toshiyuki Kudo, Maho Ogoshi, Sakae Takeuchi, Sumio Takahashi

    Gene   562 ( 1 )   62 - 9   2015.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.gene.2015.02.043

    Web of Science

    PubMed

    researchmap

  • Effect of environmental salinity on expression of adrenomedullin genes suggests osmoregulatory activity in the medaka, Oryzias latipes. Reviewed International journal

    Maho Ogoshi, Kanoko Kato, Tatsuya Sakamoto

    Zoological letters   1   12 - 12   2015

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    INTRODUCTION: The adrenomedullins (AMs) comprise a hormonal family in mammals and teleost fishes, with five members (AM1-5) found or predicted in most of the teleosts including Japanese medaka (Oryzias latipes). AM1 is known to have cardiovascular and osmoregulatory functions in mammals, but the roles of most AMs are yet to be determined. RESULTS: Using medaka, we first analyzed the tissue distribution of all five AM genes and found detectable expression in all tissues examined, with relatively high levels of AM3 and AM5 in the liver and kidney. To assess the osmoregulatory roles of these AMs, mRNA levels were examined in the brain (including the eyes), gill, liver, kidney and spleen of medaka one week after transfer from isotonic saline (11 ppt) to freshwater (0 ppt) or seawater (33 ppt). Expression of AM1 in the brain-eye increased in freshwater. The central level of AM4 (the paralog of AM1) decreased in seawater; the branchial level of AM4 decreased in freshwater and seawater, but the renal level increased in freshwater. The branchial level of AM2 increased in seawater, whereas the renal level decreased in freshwater and seawater. Expression of AM3, the AM2 paralog, decreased in the brain-eye of seawater-acclimated fish. Expression of AM5 in the brain-eye and kidney decreased in seawater. CONCLUSIONS: Except for branchial AM2, the members of AM family tend to be involved in promotion of hyper-osmoregulation and/or inhibition of hypo-osmoregulation, although each AM may play a distinct role during adaptation to different salinities.

    DOI： 10.1186/s40851-015-0012-5

    PubMed

    researchmap

  • Identification of a feather β-keratin gene exclusively expressed in pennaceous barbule cells of contour feathers in chicken. Reviewed International journal

    Kinue Kowata, Minori Nakaoka, Kaori Nishio, Ayaka Fukao, Akira Satoh, Maho Ogoshi, Sumio Takahashi, Masaoki Tsudzuki, Sakae Takeuchi

    Gene   542 ( 1 )   23 - 8   2014.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.gene.2014.03.027

    Web of Science

    PubMed

    researchmap

  • Exploring new CGRP family peptides and their receptors in vertebrates. Reviewed International journal

    Yoshio Takei, Maho Ogoshi, Shigenori Nobata

    Current protein & peptide science   14 ( 4 )   282 - 93   2013.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.2174/13892037113149990048

    Web of Science

    PubMed

    researchmap

  • Growth, energetics and the cortisol-hepatic glucocorticoid receptor axis of medaka (Oryzias latipes) in various salinities. Reviewed International journal

    Maho Ogoshi, Kanoko Kato, Hideya Takahashi, Toshitaka Ikeuchi, Tsukasa Abe, Tatsuya Sakamoto

    General and comparative endocrinology   178 ( 2 )   175 - 9   2012.9

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ygcen.2012.05.001

    Web of Science

    PubMed

    researchmap

  • Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: possible physiological roles of the calcitonin family in osmoregulation. Reviewed International journal

    Nobuo Suzuki, Toshio Sekiguchi, Honoo Satake, Kanoko Kato, Yudai Nishiyama, Hideya Takahashi, Janine A Danks, T John Martin, Atsuhiko Hattori, Masaki Nakano, Makiko Kakikawa, Sotoshi Yamada, Maho Ogoshi, Susumu Hyodo, Yoko Yamaguchi, Vishwajit S Chowdhury, Kazuichi Hayakawa, Hisayuki Funahashi, Tatsuya Sakamoto, Yuichi Sasayama

    Gene   499 ( 2 )   326 - 31   2012.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.gene.2012.03.042

    Web of Science

    PubMed

    researchmap

  • Rapid signaling of steroid hormones in the vertebrate nervous system. Reviewed International journal

    Hirotaka Sakamoto, Hideya Takahashi, Ken-Ichi Matsuda, Mayumi Nishi, Keiko Takanami, Maho Ogoshi, Tatsuya Sakamoto, Mitsuhiro Kawata

    Frontiers in bioscience (Landmark edition)   17 ( 3 )   996 - 1019   2012.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.2741/3970

    Web of Science

    PubMed

    researchmap

  • 異なる浸透圧におけるメダカの成長と体液調節・エネルギー代謝

    御輿 真穂, 坂本 竜哉

    比較内分泌学   38 ( 147 )   209 - 211   2012

    　More details

    Authorship：Lead author   Language：Japanese   Publisher：Japan Society for Comparative Endocrinology  

    DOI： 10.5983/nl2008jsce.38.209

    CiNii Article

    CiNii Books

    researchmap

    Other Link： https://jlc.jst.go.jp/DN/JALC/10013529337?from=CiNii

  • O2-filled swimbladder employs monocarboxylate transporters for the generation of O2 by lactate-induced root effect hemoglobin. Reviewed International journal

    Takahiro Umezawa, Akira Kato, Maho Ogoshi, Kayoko Ookata, Keijiro Munakata, Yoko Yamamoto, Zinia Islam, Hiroyuki Doi, Michael F Romero, Shigehisa Hirose

    PloS one   7 ( 4 )   e34579   2012

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0034579

    Web of Science

    PubMed

    researchmap

  • Hemolymph osmotic, ionic status, and branchial Na+/K+-ATPase activity under varying environmental conditions in the intertidal grapsid crab, Gaetice depressusd. Reviewed

    T. Nanba, H. Takahashi, T. Abe, W. Godo, M. Ogoshi, H. Sakamoto, N. Tsutsui, T. Sakamoto

    International Aquatic Research. 4 (2012) 1-12.   2012

    　More details

  • Corticosteroids stimulate the amphibious behavior in mudskipper: potential role of mineralocorticoid receptors in teleost fish. Reviewed International journal

    Tatsuya Sakamoto, Chie Mori, Shogo Minami, Hideya Takahashi, Tsukasa Abe, Daisuke Ojima, Maho Ogoshi, Hirotaka Sakamoto

    Physiology & behavior   104 ( 5 )   923 - 8   2011.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.physbeh.2011.06.002

    Web of Science

    PubMed

    researchmap

  • Differential expression of Na+-Cl- cotransporter and Na+-K+-Cl- cotransporter 2 in the distal nephrons of euryhaline and seawater pufferfishes. Reviewed International journal

    Akira Kato, Takayuki Muro, Yuuri Kimura, Shanshan Li, Zinia Islam, Maho Ogoshi, Hiroyuki Doi, Shigehisa Hirose

    American journal of physiology. Regulatory, integrative and comparative physiology   300 ( 2 )   R284-97 - R297   2011.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1152/ajpregu.00725.2009

    Web of Science

    PubMed

    researchmap

  • Biological functions of adrenomedullin family in teleost fish Reviewed

    Ogoshi Maho, Nobata Shigenori, Inoue Koji, Nag Kakon, Takei Yoshio, Hirose Shigehisa

    ENDOCRINE JOURNAL   57   S445   2010.3

    　More details

  • Molecular and functional evolution of the adrenomedullin family in vertebrates: What do fish studies tell us?

    Yoshio Takei, Maho Ogoshi, Marty, K.S. Wong, Shigenori Nobata

    The Calcitonin Gene-related Peptide Family: Form, Function and Future Perspectives   1 - 21   2010.1

    　More details

  • Identification of renal transporters involved in sulfate excretion in marine teleost fish. Reviewed International journal

    Akira Kato, Min-Hwang Chang, Yukihiro Kurita, Tsutomu Nakada, Maho Ogoshi, Takeru Nakazato, Hiroyuki Doi, Shigehisa Hirose, Michael F Romero

    American journal of physiology. Regulatory, integrative and comparative physiology   297 ( 6 )   R1647-59 - R1659   2009.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1152/ajpregu.00228.2009

    Web of Science

    PubMed

    researchmap

  • Potent osmoregulatory actions of homologous adrenomedullins administered peripherally and centrally in eels. Reviewed International journal

    Maho Ogoshi, Shigenori Nobata, Yoshio Takei

    American journal of physiology. Regulatory, integrative and comparative physiology   295 ( 6 )   R2075-83 - R2083   2008.12

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1152/ajpregu.90688.2008

    Web of Science

    PubMed

    researchmap

  • Central and peripheral cardiovascular actions of adrenomedullin 5, a novel member of the calcitonin gene-related peptide family, in mammals Reviewed

    Y. Takei, H. Hashimoto, K. Inoue, T. Osaki, K. Yoshizawa-Kumagaye, M. Tsunemi, T. X. Watanabe, M. Ogoshi, N. Minamino, Y. Ueta

    Journal of Endocrinology   197 ( 2 )   391 - 400   2008.5

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1677/JOE-07-0541

    Scopus

    PubMed

    researchmap

  • Potent cardiovascular actions of homologous adrenomedullins in eels. Reviewed International journal

    Shigenori Nobata, Maho Ogoshi, Yoshio Takei

    American journal of physiology. Regulatory, integrative and comparative physiology   294 ( 5 )   R1544-53 - R1553   2008.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1152/ajpregu.00707.2007

    Web of Science

    PubMed

    researchmap

  • A 'reverse' phylogenetic approach for identification of novel osmoregulatory and cardiovascular hormones in vertebrates. Reviewed International journal

    Yoshio Takei, Maho Ogoshi, Koji Inoue

    Frontiers in neuroendocrinology   28 ( 4 )   143 - 60   2007.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.yfrne.2007.05.001

    Web of Science

    PubMed

    researchmap

  • Fish calcitonin receptor has novel features Reviewed

    Kakon Nag, Akira Kato, Naznin Sultana, Maho Ogoshi, Yoshio Takei, Shigehisa Hirose

    GENERAL AND COMPARATIVE ENDOCRINOLOGY   154 ( 1-3 )   48 - 58   2007.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ygcen.2007.06.017

    Web of Science

    PubMed

    researchmap

  • Evolutionary history of the calcitonin gene-related peptide family in vertebrates revealed by comparative genomic analyses. Reviewed International journal

    Maho Ogoshi, Koji Inoue, Kiyoshi Naruse, Yoshio Takei

    Peptides   27 ( 12 )   3154 - 64   2006.12

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.peptides.2006.09.011

    Web of Science

    PubMed

    researchmap

  • Evolutionary history of the CGRP family in vertebrates revealed by comparative genomic analyses Reviewed

    Ogoshi Maho, Inoue Koji, Naruse Kiyoshi, Takei Yoshio

    ZOOLOGICAL SCIENCE   23 ( 12 )   1210   2006.12

    　More details

    Authorship：Lead author  

    Web of Science

    researchmap

  • Contribution of comparative fish studies to general endocrinology: structure and function of some osmoregulatory hormones. Reviewed International journal

    Yoshio Takei, Akatsuki Kawakoshi, Takehiro Tsukada, Shinya Yuge, Maho Ogoshi, Koji Inoue, Susumu Hyodo, Hideo Bannai, Satoru Miyano

    Journal of experimental zoology. Part A, Comparative experimental biology   305 ( 9 )   787 - 98   2006.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Fish endocrinologists are commonly motivated to pursue their research driven by their own interests in these aquatic animals. However, the data obtained in fish studies not only satisfy their own interests but often contribute more generally to the studies of other vertebrates, including mammals. The life of fishes is characterized by the aquatic habitat, which demands many physiological adjustments distinct from the terrestrial life. Among them, body fluid regulation is of particular importance as the body fluids are exposed to media of varying salinities only across the thin respiratory epithelia of the gills. Endocrine systems play pivotal roles in the homeostatic control of body fluid balance. Judging from the habitat-dependent control mechanisms, some osmoregulatory hormones of fish should have undergone functional and molecular evolution during the ecological transition to the terrestrial life. In fact, water-regulating hormones such as vasopressin are essential for survival on the land, whereas ion-regulating hormones such as natriuretic peptides, guanylins and adrenomedullins are diversified and exhibit more critical functions in aquatic species. In this short review, we introduce some examples illustrating how comparative fish studies contribute to general endocrinology by taking advantage of such differences between fishes and tetrapods. In a functional context, fish studies often afford a deeper understanding of the essential actions of a hormone across vertebrate taxa. Using the natriuretic peptide family as an example, we suggest that more functional studies on fishes will bring similar rewards of understanding. At the molecular level, recent establishment of genome databases in fishes and mammals brings clues to the evolutionary history of hormone molecules via a comparative genomic approach. Because of the functional and molecular diversification of ion-regulating hormones in fishes, this approach sometimes leads to the discovery of new hormones in tetrapods as exemplified by adrenomedullin 2.

    DOI： 10.1002/jez.a.309

    PubMed

    researchmap

  • Contribution of comparative fish studies to general endocrinology: structure and function of some osmoregulatory hormones Reviewed

    Takei Yoshio, Kawakoshi Akatsuki, Tsukada Takehiro, Yuge Shinya, Ogoshi Maho, Inoue Koji, Hyodo Susumu, Bannai Hideo, Miyano Satoru

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY   305A ( 9 )   787 - 798   2006.9

    　More details

  • Identification of novel adrenomedullin in mammals: a potent cardiovascular and renal regulator. Reviewed International journal

    Yoshio Takei, Koji Inoue, Maho Ogoshi, Tetsushi Kawahara, Hideo Bannai, Satoru Miyano

    FEBS letters   556 ( 1-3 )   53 - 8   2004.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0014-5793(03)01368-1

    Web of Science

    PubMed

    researchmap

  • Identification of a novel adrenomedullin gene family in teleost fish. Reviewed International journal

    Maho Ogoshi, Koji Inoue, Yoshio Takei

    Biochemical and biophysical research communications   311 ( 4 )   1072 - 7   2003.11

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2003.10.111

    Web of Science

    PubMed

    researchmap

▼display all

Books

  • Handbook of hormones : comparative endocrinology for basic and clinical research -2nd Edition

    安東, 宏徳, Ukena, Kazuyoshi, Nagata, Shinji（ Role： Joint author ,  Calcitonin Gene-Related Peptide; Calcitonin Receptor-Stimulating Peptide; Adrenomedullin; Adrenomedullin 2 and 5; Amylin）

    Academic Press  2021.7  （ ISBN:9780323851947 ）

    　More details

    Total pages：xxvii, p. 619-1113   Language：English Book type：Scholarly book

    researchmap

  • ホメオスタシスと適応 : 恒

    海谷, 啓之, 内山, 実（ Role： Joint author ,  水・電解質代謝とホルモン）

    裳華房  2016.8  （ ISBN:9784785351182 ）

    　More details

    Total pages：xiv, 227p, 図版 [4] p   Language：Japanese Book type：Scholarly book

    CiNii Books

    researchmap

  • 基礎生物科学

    高橋, 純夫, 鷲谷, いづみ（ Role： Joint author ,  循環と呼吸、浸透圧調節と腎臓）

    培風館  2016.7  （ ISBN:9784563078218 ）

    　More details

    Total pages：vi, 238p   Language：Japanese Book type：Textbook, survey, introduction

    CiNii Books

    researchmap

  • Handbook of hormones : comparative endocrinology for basic and clinical research

    竹井, 祥郎, 安東, 宏徳, 筒井, 和義（ Role： Joint author ,  Calcitonin Gene-Related Peptide; Calcitonin Receptor-Stimulating Peptide; Adrenomedullin; Adrenomedullin 2 and 5; Amylin）

    Academic Press is an imprint of Elsevier  2016  （ ISBN:9780128010280 ）

    　More details

    Total pages：xxvii, 646 p.   Language：English Book type：Scholarly book

    CiNii Books

    researchmap

  • Eel physiology

    Trischitta, Francesca, 竹井, 祥郎, Sébert, Philippe（ Role： Joint author ,  Endocrine control of osmoregulation）

    CRC Press  2014  （ ISBN:9781466598270 ）

    　More details

    Total pages：viii, 370 p.   Language：English Book type：Scholarly book

    CiNii Books

    researchmap

  • 現代生物学入門

    岡山大学生物学教科書作成委員会, 高橋, 純夫, 阿保, 達彦（ Role： Contributor ,  浸透圧応答）

    岡山大学出版会  2011.3  （ ISBN:9784904228197 ）

    　More details

    Total pages：185p   Language：Japanese Book type：Textbook, survey, introduction

    CiNii Books

    researchmap

  • The Calcitonin Gene-Related Peptide Family: Form, Function and Future Perspectives

    Hay DL, Dickerson I（ Role： Joint author ,  Molecular and functional evolution of the adrenomedullin family in vertebrates: What do fish tell us?）

    Springer Verlag  2009 

    　More details

    Book type：Scholarly book

    researchmap

▼display all

MISC

  • ツメガエル属におけるAdrenomedullin 5の局在と免疫に関する機能

    御輿真穂, 御輿真穂, 山浦晋平, 青柳康太, 荒木文平, 相澤清香, 相澤清香, 竹内栄, 竹内栄, 高橋純夫, 高橋純夫

    岡山実験動物研究会報   ( 35 )   2019

    　More details

  • ニワトリにおける遅羽性K遺伝子の作用メカニズムの解析

    岡村彩子, 増本絢音, 竹之内惇, 相澤清香, 御輿真穂, 御輿真穂, 高橋純夫, 高橋純夫, 都築政起, 竹内栄, 竹内栄

    岡山実験動物研究会報   ( 34 )   2018

    　More details

  • ラット隆起部におけるニューロメジンUの発現とその制御メカニズム

    相澤清香, 顧ていてい, 神之田有紗, 藤岡竜矢, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報   ( 34 )   2018

    　More details

  • 海洋生物の適応戦略-新規技術・現象からの新展開-水棲脊椎動物におけるアドレノメデュリンファミリーの新たな特徴

    御輿真穂

    月刊海洋   49 ( 5 )   2017

    　More details

  • マウス子宮内膜におけるTGF-β遺伝子の発現制御

    吉田すみれ, 相澤清香, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報   ( 33 )   2017

    　More details

  • ラット脳下垂体隆起部の新規生理機能の探索

    相澤清香, 坂井田初季, 坂田一郎, 坂井貴文, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報   ( 33 )   2017

    　More details

  • Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis

    相澤清香, 檜垣佑理子, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報   ( 33 )   2017

    　More details

  • ウズラPBCF遺伝子の構造と発現

    釘本綾子, 相澤清香, 御輿真穂, 高橋純夫, 竹内栄

    岡山実験動物研究会報   32 ( 32 )   19 - 21   2016

    　More details

  • マウス子宮内膜細胞におけるKallikreinの発現制御の解析

    徳森萌美, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報   31 ( 31 )   37 - 39   2015

    　More details

  • マウス卵巣機能制御における転写因子Runx3の生理的役割

    小島史也, 斉藤優佳, 土家由紀子, 御輿真穂, 御輿真穂, 竹内栄, 竹内栄, 高橋純夫, 高橋純夫

    岡山実験動物研究会報   ( 31 )   2015

    　More details

  • ニワトリにおける羽色調節の品種差

    高橋徹, 西尾香織, 御輿真穂, 高橋純夫, 竹内栄

    岡山実験動物研究会報   ( 31 )   2015

    　More details

  • マウス卵巣顆粒膜細胞におけるインスリン様成長因子I遺伝子の発現

    林紗代, 小島史也, 御輿真穂, 竹内栄, 高橋純夫

    岡山実験動物研究会報   ( 31 )   2015

    　More details

  • Runx3 Regulates Ovulation and Steroidogenesis in Granulosa Cells in Mice

    Fumiya Ojima, Yuka Saito, Yukiko Tsuchiya, Kenichi Inagaki, Eri Nakamura, Fumio Otsuka, Maho Ogoshi, Sakae Takeuchi, Sumio Takahashi

    ENDOCRINE REVIEWS   35 ( 3 )   2014.6

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)  

    Web of Science

    researchmap

  • ニワトリにおける羽色調節の品種差

    高橋徹, 西尾香織, 御輿真穂, 高橋純夫, 竹内栄

    岡山実験動物研究会報   ( 30 )   2014

    　More details

  • 成鶏羽の性的二型をつくる局所シグナル系

    木幡衣惠, 西尾香織, 佐保里美, 工藤季之, 御輿真穂, 御輿真穂, 高橋純夫, 高橋純夫, 竹内栄, 竹内栄

    日本動物学会大会予稿集   85th   2014

    　More details

  • The mechanism of local blood acidification in the swimbladder by spatially organized monocarboxylate transporters

    Akira Kato, Takahiro Umezawa, Maho Ogoshi, Kayoko Ookata, Keijiro Munakata, Hiroyuki Doi, Michael F. Romero, Shigehisa Hirose

    FASEB JOURNAL   26   2012.4

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)  

    Web of Science

    researchmap

  • 第36回日本比較内分泌学会大会<br>若手交流会パネルディスカッション総括

    御輿 真穂, 日下部 誠, 今野 紀文

    比較内分泌学   38 ( 144 )   14 - 16   2012

    　More details

    Language：Japanese   Publisher：Japan Society for Comparative Endocrinology  

    CiNii Article

    researchmap

  • 脊椎動物におけるアドレノメデュリンファミリーの進化と機能

    御輿真穂

    比較内分泌学   37 ( 141 )   2011

    　More details

  • 鰾(うきぶくろ)奇網に高発現する乳酸輸送体(monocarboxylate transporters MCT1b and MCT4b)の同定と血液酸性化およびO2分泌における役割

    梅澤誉広, 御輿真穂, 土井啓行, 広瀬茂久, 加藤明

    生化学   2010

    　More details

  • 硬骨魚真骨類における新規アドレノメデュリンファミリーの分子進化および生理学的研究

    御輿 真穂

    比較内分泌学 = Comparative endocrinology   35 ( 133 )   109 - 118   2009.5

    　More details

    Language：Japanese   Publisher：日本比較内分泌学会  

    CiNii Article

    CiNii Books

    researchmap

  • Fishing novel hormone genes from fish database

    Y Takei, M Ogoshi, A Kawakoshi, K Inoue, S Hyodo

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY   305A ( 2 )   183 - 183   2006.2

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Molecular evolution of calcitonin gene-related peptide (CGRP) family.

    御輿真穂, 竹井祥郎

    生体の科学   57 ( 5 )   436 - 438   2006

    　More details

  • CHANGES IN THE EXPRESSION OF NOVEL ADRENOMEDULLIN GENES IN PUFFERFISH, TAKIFUGU RUBRIPES, AFTER TRANSFER TO DIFFERENT OSMOTIC ENVIRONMENTS

    OGOSHI Maho, INOUE Koji, HYODO Susumu, TAKEI Yoshio

    ( 20 )   21 - 21   2005.11

    　More details

  • アドレノメデュリン研究の新展開 比較内分泌学からの発信:新規アドレノメデュリン(AM2)の発見

    竹井祥郎, 御輿真穂

    分子心血管病   6 ( 5 )   2005

    　More details

  • A novel adrenomedullin family identified in fish

    Maho Ogoshi, Yoshio Takei

    ZOOLOGICAL SCIENCE   21 ( 12 )   1230 - 1230   2004.12

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)  

    Web of Science

    researchmap

  • 魚類のadrenomedullin受容体ファミリーの単離と機能解析

    KAKON N, 加藤明, 御輿真穂, 竹井祥郎, 広瀬茂久

    日本内分泌学会雑誌   80 ( 2 )   2004

    　More details

  • A Novel Adrenomedullin Family Identified in Fish(Messages from Comparative Endocrinology: Diverse Hormone Function,Symposium,PROCEEDING OF THE 75^<TH> ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN) :

    Ogoshi Maho, Takei Yoshio

    Zoological science   21 ( 12 )   1230 - 1230   2004

    　More details

    Language：English   Publisher：Zoological Society of Japan  

    CiNii Article

    CiNii Books

    researchmap

    Other Link： http://id.nii.ac.jp/1141/00038656/

▼display all

Research Projects

  • ニホンウナギの環境DNAモニタリングを通じて解明する川と海とのつながり

    2024 - 2025

    八雲環境科学振興財団  環境研究助成（特定研究） 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • 川と海のつながりを環境DNAによって評価する：ニホンウナギをシンボル種として

    2022 - 2023

    八雲環境科学振興財団  環境研究助成（特定研究） 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • 川と海のつながりを保全・回復するための研究：ニホンウナギをシンボル種として

    2016

    八雲環境科学振興財団  環境研究助成（特定研究） 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • Biological function of novel adrenomedullins in teleosts and amphibians: focusing on hematopoietic and immune system

    Grant number：26870386  2014.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Maho Ogoshi

    　 More details

    Grant amount：\3900000 （ Direct expense: \3000000 、 Indirect expense：\900000 ）

    We examined the hematopoietic function of novel adrenomedullins in vertebrates. The AM3 gene expression was induced after hemolytic anemia in teleost fish, medaka, suggesting its role in erythropoiesis. In Xenopus tropicalis, AM5 mRNA was detected in the ventral blood island at tailbud stage embryo and was expressed in adult hematopoietic stem cells.

    researchmap

  • 両生類における新型アドレノメデュリンの造血機能の解析

    2014

    山陽放送学術文化財団  学術研究助成 学術奨励賞 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • メダカ血液細胞の成熟における新型アドレノメデュリンの作用

    2014

    両備檉園記念財団  生物学研究奨励賞 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • ニホンウナギを指標とした河川生態系の健全性を評価する手法の開発と環境学習に関する研究

    2012

    八雲環境科学振興財団  環境研究助成（特定研究） 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • The possibleprincipal function of neurohypophysial hormones

    Grant number：22570065  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SAKAMOTO Tatsuya, OGOSHI Maho, SAKAMOTO Hirotaka, TAKAHASHI Hideya

    　 More details

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    Acclimation from marine to dilute environments constitutes among the most dramatic evolutionary transitions in the history of life. Such adaptations have evolved in multiple lineages, but studies of the mechanisms are limited to those using evolutionarily advanced Deuterostome (chordates) and Ecdysozoa (crustaceans). Here we examined the body-fluid homeostasis in the advanced Lophotrochozoa/mollusc, the other unexplored taxa, and its possible regulations byvasopressin/oxytocin superfamily peptides known to be implicated in fluid homeostasis in Chordata and Arthropoda. The hemolymph osmotic and ionic status in the euryhaline cephalopod (Octopus ocellatus) following transfer from normal seawater (30 ppt) to low (20 ppt) salinity indicate the hyperosmo- and hyperiono-regulatory abilities for more than 1 week, as in crustaceans and teleost fish. While ventilation frequency decreased by 1 day, Na+/K+-ATPase activity, which has been implicated in ion transport generally, was induced in two of the eight posterior gills after 1 week. Furthermore, the octopuses were intravenously injected with 1 or 100 ng/g octopressin or cephalotocin, cephalopod vasopressin/oxytocin orthologs. After 1 day, octopressin, not cephalotocin, decreased the hemolymph osmolality and Ca concentrations as well as urinary Na concentrations. These data provide evidence for convergent evolution in the hyper-ionoregulatory mechanisms and the coordination by conserved molecules.

    researchmap

  • Biological function of novel adrenomedullin in vertebrates : a reverse-phylogenic approach

    Grant number：22870020  2010 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity Start-up

    OGOSHI Maho, SAKAMOTO Tatsuya

    　 More details

    Grant amount：\3146000 （ Direct expense: \2420000 、 Indirect expense：\726000 ）

    We have clarified that all five members of adrenomedullin(AM) family were expressed in tissues of medaka(Oryzias latipes) including osmoregulatory organs. The expressions of AM1, AM3, AM4 and AM5 were increased after transfer to freshwater and decreased in seawater, whereas that of AM2 was increased in seawater. The obtained data suggest that AM family genes except for AM2 are involved in freshwater adaptation, whereas AM2 is related to the adaptation to seawater.

    researchmap

  • 脊椎動物におけるアドレノメデュリンファミリーの新規受容体の探索

    Grant number：09J08363  2009

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    御輿 真穂

    　 More details

    Grant amount：\1000000 （ Direct expense: \1000000 ）

    本年度は、発現クローニングによって受容体遺伝子を同定するための準備段階として、まず対照として用いる既知のAM受容体遺伝子(CLR, calcitonin receptor-like receptorおよびRAMP, receptor activity-modifying protein)のウナギにおけるホモログのcDNAクローニングを行った。その結果、CLR1遺伝子の配列を決定し、この発現分布を調べたところ、浮き袋の奇網などの血管が豊富な組織に存在することがわかった。新規受容体は既知の受容体遺伝子と似た構造をもつと考えられるため、得られたアミノ酸配列をもとに、現在利用できる硬骨魚類のゲノムデータベースを検索したが、相同性による新規遺伝子の候補は見つけることができなかった。現在、タンパク質のアフィニティによる結合アッセイを行うため、RI標識したリガンド分子を準備中である。
    また、ウナギにおいてはAM1よりもAM2/AM5のほうがより強力な生理作用を示したが、このような現象が他の硬骨魚類でも一般的であることを確認するため、ウナギ以外の種においてもAMファミリーの機能解析を行った。ゼブラフィッシュの受精卵を用いてAMファミリーの各遺伝子をノックダウンすることで、発生・分化におけるAMファミリーの機能を解析しようと試みた。ゲノムデータベースの解析によって、ゼブラフィッシュにはAM2遺伝子が存在しないことが明らかになったため、AM2のパラログであるAM3、およびAM1、AM5の各mRNAに結合するアンチセンスモルフォリノオリゴを設計し、受精卵へのマイクロインジェクションを行った。その結果、AM1およびAM3遺伝子をノックダウンした群では血管分化の阻害が観察された。より顕著な表現型がみられたのはAM5をノックダウンした群であり、この群では頭部の水腫、赤血球の減少、運動性の著しい低下など多くの異常が観察された。現在、これらの部位の発生にかかわる遺伝子とAM5遺伝子との相互作用について解析を行っている。

    researchmap

  • トラフグにおける新規アドレノメデュリンファミリーの浸透圧調節作用

    Grant number：05J12006  2005 - 2007

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    御輿 真穂

    　 More details

    Grant amount：\2700000 （ Direct expense: \2700000 ）

    前年度までの研究によって、脊椎動物のアドレノメデュリン(AM)ファミリーは、3つの祖先型分子(AM1、AM2、AM5)から進化してきたことがわかったため、今年度は真骨類におけるAMファミリーの作用をより詳細に明らかにする目的で、ウナギを用いてAMを投与する生理実験を行った。ウナギの背大動脈・食道・膀胱にそれぞれカニューレを挿入し、血管カニューレからホルモンを投与すると同時に血圧を測定した。食道および膀胱カニューレからは飲水量および尿量を測定した。投与したホルモンは、5種類のAMのうち、祖先型分子が異なるAM1、AM2、AM5を選んだ。その結果、血圧・飲水量への作用はAM2とAM5が最も強く、これまでに知られていたどのホルモンよりも強力な降圧作用、飲水促進作用を示した。哺乳類において強い作用を示すAM1は、ウナギにおいては弱い作用しか示さなかった。また尿量についてはAM2が抗利尿作用をもつことが明らかとなった。これらの結果により、真骨類であるウナギにおいては、AM2およびAM5が浸透圧調節作用をもつことがわかった。
    また、AM2およびAM5による飲水促進作用がどのような経路によって起こるのかを調べるため、ウナギの第三脳室および第四脳室内にホルモンを投与する実験を行った。その結果、飲水促進ホルモンであるアンジオテンシンIIが強力に飲水を誘起したのに対し、ウナギAMはどちらの脳室内投与によっても飲水を促進しなかった。このことから、AM2、AM5は脳室周囲器官を通じて飲水を促進するのではなく、血中から血液脳関門のない神経核を介して作用していることが示唆された。
    これまでに同定されている真骨類AMの受容体とリガンドとの親和性はAM1が最も高く、本研究によって得られた結果とは一致しない。したがって、真骨類にはAM2またはAM5に特異的な未知の受容体が存在する可能性が高い。

    researchmap

▼display all

 

Class subject in charge

  • Biology (Animals and Plants) （2024academic year） 1st semester  - 金1～2

  • Experimental Course (Molecular Biology) （2024academic year） Fourth semester  - 木5～8

  • Comparative Endocrinology （2024academic year） Late  - 火1～2

  • Special Lecture in Biology （2024academic year） Summer concentration  - その他

  • Cell Biology IIB （2024academic year） Second semester  - 水1～2

  • Biology (Animals and Plants) （2023academic year） 1st semester  - 金1～2

  • Experimental Course (Molecular Biology) （2023academic year） Fourth semester  - 木5～8

  • Comparative endocrinology （2023academic year） Late  - 火1～2

  • Comparative Endocrinology （2023academic year） Late  - 火1～2

  • Special Lecture in Biology （2023academic year） Summer concentration  - その他

  • Cell Biology IIB （2023academic year） Second semester  - 水1～2

  • Biology (Animals and Plants) （2022academic year） 1st semester  - 金1～2

  • Special Lecture in Biology （2022academic year） Summer concentration  - その他

  • Cell Biology IIB （2022academic year） Second semester  - 水1～2

  • Biology (Animals and Plants) （2021academic year） 1st semester  - 金1～2

  • Comparative endocrinology （2021academic year） Late  - 火1,火2

  • Cell Biology 2 （2021academic year） 1st and 2nd semester  - 水1,水2

  • Cell Biology IIB （2021academic year） Second semester  - 水1,水2

  • Biology (Animals and Plants) （2020academic year） 1st semester  - 金1,金2

  • Comparative endocrinology （2020academic year） Late  - 木1,木2

  • Cell Biology 2 （2020academic year） 1st and 2nd semester  - 水1,水2

  • Cell Biology IIB （2020academic year） Second semester  - 水1,水2

▼display all