Updated on 2025/07/16

写真a

 
YOSHII Taishi
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Professor
Position
Professor
External link

Degree

  • 博士(理学) ( 岡山大学 )

Research Interests

  • Circadian clock

  • Chronobiology

  • 体内時計

  • 概日リズム

  • Drosophila melanogaster

Research Areas

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

Education

  • Okayama University   大学院自然科学研究科  

    2003 - 2006

      More details

  • Yamaguchi University   大学院理工学研究科  

    2001 - 2003

      More details

  • Yamaguchi University   理学部  

    - 2001

      More details

Research History

  • Okayama University   学術研究院環境生命自然科学学域   Professor

    2022.4

      More details

    Country:Japan

    researchmap

  • Okayama University   The Graduate School of Natural Science and Technology   Associate Professor

    2014.2 - 2022.3

      More details

  • Okayama University   The Graduate School of Natural Science and Technology   Assistant Professor

    2011 - 2014

      More details

  • ヴュルツブルク大学   助教

    2010 - 2011

      More details

  • レーゲンスブルク大学   博士研究員

    2006 - 2009

      More details

Professional Memberships

Committee Memberships

  • 日本動物学会   IT委員会  

    2016   

      More details

    Committee type:Academic society

    researchmap

  • 日本動物学会   広報委員  

    2016   

      More details

    Committee type:Academic society

    researchmap

  • SRBR   The Society for Research on Biological Rhythms Logo committee  

    2016   

      More details

    Committee type:Academic society

    researchmap

  • 日本動物学会中国四国支部   庶務幹事  

    2014 - 2016   

      More details

    Committee type:Academic society

    researchmap

 

Papers

  • Neurotransmitter and Receptor Mapping in Drosophila Circadian Clock Neurons via T2A-GAL4 Screening. Reviewed International journal

    Ayumi Fukuda, Aika Saito, Taishi Yoshii

    Journal of biological rhythms   7487304251349887 - 7487304251349887   2025.7

     More details

    Authorship:Corresponding author   Language:English  

    The circadian neuronal network in the brain comprises central pacemaker neurons and associated input and output pathways. These components work together to generate coherent rhythmicity, synchronize with environmental time cues, and convey circadian information to downstream neurons that regulate behaviors such as the sleep/wake cycle. To mediate these functions, neurotransmitters and neuromodulators play essential roles in transmitting and modulating signals between neurons. In Drosophila melanogaster, approximately 240 brain neurons function as clock neurons. Previous studies have identified several neurotransmitters and neuromodulators, including the Pigment-dispersing factor (PDF) neuropeptide, along with their corresponding receptors in clock neurons. However, our understanding of the neurotransmitters and receptors involved in the circadian system remains incomplete. In this study, we conducted a T2A-GAL4-based screening for neurotransmitter and receptor genes expressed in clock neurons. We identified 2 neurotransmitter-related genes and 22 receptor genes. Notably, while previous studies had reported the expression of 6 neuropeptide receptor genes in large ventrolateral neurons (l-LNv), we also found that 14 receptor genes-including those for dopamine, serotonin, and γ-aminobutyric acid-are expressed in l-LNv neurons. These findings suggest that l-LNv neurons serve as key integrative hubs within the circadian network, receiving diverse external signals.

    DOI: 10.1177/07487304251349887

    PubMed

    researchmap

  • Neuromedin U Deficiency Disrupts Daily Testosterone Fluctuation and Reduces Wheel-Running Activity in Rats. Reviewed International journal

    Mai Otsuka, Yu Takeuchi, Maho Moriyama, Sakura Egoshi, Yuki Goto, Tingting Gu, Atsushi P Kimura, Shogo Haraguchi, Taishi Yoshii, Sakae Takeuchi, Makoto Matsuyama, George E Bentley, Sayaka Aizawa

    Endocrinology   166 ( 8 )   2025.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The objective of this study was to elucidate the role of endogenous Neuromedin U (NMU) in rats by performing NMU knockout (KO). Male, but not female NMU KO rats exhibited decreased wheel-running activity vs wildtype (WT), although overall home cage activity was not affected. Plasma testosterone in WT rats varied significantly over the course of a day, with a peak at ZT1 and a nadir at ZT18, whereas in NMU KO rats testosterone remained stable throughout the day. Chronic administration of testosterone restored wheel-running activity in NMU KO rats to the same level as in WT rats, suggesting that the decrease in wheel-running activity in NMU KO rats is due to the disruption of the diurnal change of testosterone. Accordingly, expression of the luteinizing hormone beta subunit (Lhb) mRNA in the pars distalis of anterior pituitary was significantly lower in NMU KO rats; immunostaining revealed that the size of luteinizing hormone (LH)-expressing cells was also relatively small in those animals. In the brain of male WT rats, Nmu was highly expressed in the pars tuberalis, and the NMU receptor Nmur2 was highly expressed in the ependymal cell layer of the third ventricle. This study reveals a novel function of NMU and indicates that endogenous NMU in rats plays a role in the regulation of motivated activity via regulation of testosterone.

    DOI: 10.1210/endocr/bqaf102

    PubMed

    researchmap

  • Effect of temperature cycles on the sleep-like state in Hydra vulgaris Reviewed

    Aya Sato, Manabu Sekiguchi, Koga Nakada, Taishi Yoshii, Taichi Q. Itoh

    Zoological Letters   11 ( 1 )   2025.1

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Background

    Sleep is a conserved physiological phenomenon across species. It is mainly controlled by two processes: a circadian clock that regulates the timing of sleep and a homeostat that regulates the sleep drive. Even cnidarians, such as Hydra and jellyfish, which lack a brain, display sleep-like states. However, the manner in which environmental cues affect sleep-like states in these organisms remains unknown. In the present study, we investigated the effects of light and temperature cycles on the sleep-like state in Hydra vulgaris.

    Results

    Our findings indicate that Hydra responds to temperature cycles with a difference of up to 5° C, resulting in decreased sleep duration under light conditions and increased sleep duration in dark conditions. Furthermore, our results reveal that Hydra prioritizes temperature changes over light as an environmental cue. Additionally, our body resection experiments show tissue-specific responsiveness in the generation ofthe sleep-like state under different environmental cues. Specifically, the upper body can generate the sleep-like state in response to a single environmental cue. In contrast, the lower body did not respond to 12-h light–dark cycles at a constant temperature.

    Conclusions

    These findings indicate that both light and temperature influence the regulation of the sleep-like state in Hydra. Moreover, these observations highlight the existence of distinct regulatory mechanisms that govern patterns of the sleep-like state in brainless organisms, suggesting the potential involvement of specific regions for responsiveness of environmental cues for regulation of the sleep-like state.

    DOI: 10.1186/s40851-025-00248-1

    researchmap

    Other Link: https://link.springer.com/article/10.1186/s40851-025-00248-1/fulltext.html

  • A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster. Reviewed International journal

    Yuto Yoshinari, Takashi Nishimura, Taishi Yoshii, Shu Kondo, Hiromu Tanimoto, Tomoe Kobayashi, Makoto Matsuyama, Ryusuke Niwa

    Nature communications   15 ( 1 )   10819 - 10819   2024.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to protein deficiency are well-studied, there is a significant gap in our understanding of how organisms adaptively suppress excessive protein intake. In the present study, utilizing the fruit fly, Drosophila melanogaster, we discover that the peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells in response to a high-protein diet (HPD), is vital for suppressing overconsumption of protein. Gut-derived CCHa1 is received by a small subset of enteric neurons that produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment of the CCHa1-mediated gut-enteric neuronal axis results in ammonia accumulation and a shortened lifespan under HPD conditions. Collectively, our findings unravel the crosstalk of gut hormone and neuronal pathways that orchestrate physiological responses to prevent and adapt to dietary protein overload.

    DOI: 10.1038/s41467-024-55050-y

    PubMed

    researchmap

  • Synaptic and peptidergic connectomes of theDrosophilacircadian clock Reviewed International journal

    Nils Reinhard, Ayumi Fukuda, Giulia Manoli, Emilia Derksen, Aika Saito, Gabriel Möller, Manabu Sekiguchi, Dirk Rieger, Charlotte Helfrich-Förster, Taishi Yoshii, Meet Zandawala

    Nature communications   15 ( 1 )   10392 - 10392   2024.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Cold Spring Harbor Laboratory  

    The circadian clock of animals regulates various physiological and behavioral processes in anticipation of, and adaptation to, daily environmental fluctuations. Consequently, the circadian clock and its output pathways play a pivotal role in maintaining homeostasis and optimizing daily functioning. To obtain novel insights into how diverse rhythmic physiology and behaviors are orchestrated, we have generated the first comprehensive connectivity map of an animal circadian clock using theDrosophilaFlyWire brain connectome. We reveal hitherto unknown extensive contralateral synaptic connectivity between the clock neurons, which might contribute to the robustness of the clock by synchronizing clock neurons across the two hemispheres. In addition, we discover novel direct and indirect light input pathways to the clock neurons that could facilitate clock entrainment. Intriguingly, we observe sparse monosynaptic connectivity between clock neurons and downstream higher-order brain centers and neurosecretory cells known to regulate several behaviors and physiology. Therefore, we integrated single-cell transcriptomic analysis and receptor mapping to additionally decipher paracrine peptidergic signaling between clock neurons and with neurosecretory cells. Our analyses identified additional novel neuropeptides expressed in clock neurons and suggest that peptidergic signaling greatly enriches the interconnectivity within the clock network. Neuropeptides also form the basis of output pathways which regulate rhythmic hormonal signaling. TheDrosophilacircadian clock and neurosecretory center connectomes provide the framework to understand more complex clock and hormonal networks, respectively, as well as the rhythmic processes regulated by them.

    DOI: 10.1038/s41467-024-54694-0

    PubMed

    researchmap

  • A Detailed Re-Examination of the Period Gene Rescue Experiments Shows That Four to Six Cryptochrome-Positive Posterior Dorsal Clock Neurons (DN1p) of Drosophila melanogaster Can Control Morning and Evening Activity Reviewed

    Manabu Sekiguchi, Nils Reinhard, Ayumi Fukuda, Shun Katoh, Dirk Rieger, Charlotte Helfrich-Förster, Taishi Yoshii

    Journal of Biological Rhythms   2024.7

     More details

    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:SAGE Publications  

    Animal circadian clocks play a crucial role in regulating behavioral adaptations to daily environmental changes. The fruit fly Drosophila melanogaster exhibits 2 prominent peaks of activity in the morning and evening, known as morning (M) and evening (E) peaks. These peaks are controlled by 2 distinct circadian oscillators located in separate groups of clock neurons in the brain. To investigate the clock neurons responsible for the M and E peaks, a cell-specific gene expression system, the GAL4-UAS system, has been commonly employed. In this study, we re-examined the two-oscillator model for the M and E peaks of Drosophila by utilizing more than 50 Gal4 lines in conjunction with the UAS-period16 line, which enables the restoration of the clock function in specific cells in the period ( per) null mutant background. Previous studies have indicated that the group of small ventrolateral neurons (s-LNv) is responsible for controlling the M peak, while the other group, consisting of the 5th ventrolateral neuron (5th LNv) and the three cryptochrome (CRY)-positive dorsolateral neurons (LNd), is responsible for the E peak. Furthermore, the group of posterior dorsal neurons 1 (DN1p) is thought to also contain M and E oscillators. In this study, we found that Gal4 lines directed at the same clock neuron groups can lead to different results, underscoring the fact that activity patterns are influenced by many factors. Nevertheless, we were able to confirm previous findings that the entire network of circadian clock neurons controls M and E peaks, with the lateral neurons playing a dominant role. In addition, we demonstrate that 4 to 6 CRY-positive DN1p cells are sufficient to generate M and E peaks in light-dark cycles and complex free-running rhythms in constant darkness. Ultimately, our detailed screening could serve as a catalog to choose the best Gal4 lines that can be used to rescue per in specific clock neurons.

    DOI: 10.1177/07487304241263130

    researchmap

    Other Link: https://journals.sagepub.com/doi/full-xml/10.1177/07487304241263130

  • The Trissin/TrissinR signaling pathway in the circadian network regulates evening activity in Drosophila melanogaster under constant dark conditions Reviewed

    Manabu Sekiguchi, Shun Katoh, Tatsuya Yokosako, Aika Saito, Momoka Sakai, Ayumi Fukuda, Taichi Q. Itoh, Taishi Yoshii

    Biochemical and Biophysical Research Communications   704   149705 - 149705   2024.4

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrc.2024.149705

    researchmap

  • Characterization of clock-related proteins and neuropeptides in Drosophila littoralis and their putative role in diapause. Reviewed International journal

    Giulia Manoli, Meet Zandawala, Taishi Yoshii, Charlotte Helfrich-Förster

    The Journal of comparative neurology   531 ( 15 )   1525 - 1549   2023.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Insects from high latitudes spend the winter in a state of overwintering diapause, which is characterized by arrested reproduction, reduced food intake and metabolism, and increased life span. The main trigger to enter diapause is the decreasing day length in summer-autumn. It is thus assumed that the circadian clock acts as an internal sensor for measuring photoperiod and orchestrates appropriate seasonal changes in physiology and metabolism through various neurohormones. However, little is known about the neuronal organization of the circadian clock network and the neurosecretory system that controls diapause in high-latitude insects. We addressed this here by mapping the expression of clock proteins and neuropeptides/neurohormones in the high-latitude fly Drosophila littoralis. We found that the principal organization of both systems is similar to that in Drosophila melanogaster, but with some striking differences in neuropeptide expression levels and patterns. The small ventrolateral clock neurons that express pigment-dispersing factor (PDF) and short neuropeptide F (sNPF) and are most important for robust circadian rhythmicity in D. melanogaster virtually lack PDF and sNPF expression in D. littoralis. In contrast, dorsolateral clock neurons that express ion transport peptide in D. melanogaster additionally express allatostatin-C and appear suited to transfer day-length information to the neurosecretory system of D. littoralis. The lateral neurosecretory cells of D. littoralis contain more neuropeptides than D. melanogaster. Among them, the cells that coexpress corazonin, PDF, and diuretic hormone 44 appear most suited to control diapause. Our work sets the stage to investigate the roles of these diverse neuropeptides in regulating insect diapause.

    DOI: 10.1002/cne.25522

    PubMed

    researchmap

  • A four-oscillator model of seasonally adapted morning and evening activities in Drosophila melanogaster. Invited Reviewed International journal

    Taishi Yoshii, Aika Saito, Tatsuya Yokosako

    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology   2023.5

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    The fruit fly Drosophila melanogaster exhibits two activity peaks, one in the morning and another in the evening. Because the two peaks change phase depending on the photoperiod they are exposed to, they are convenient for studying responses of the circadian clock to seasonal changes. To explain the phase determination of the two peaks, Drosophila researchers have employed the two-oscillator model, in which two oscillators control the two peaks. The two oscillators reside in different subsets of neurons in the brain, which express clock genes, the so-called clock neurons. However, the mechanism underlying the activity of the two peaks is complex and requires a new model for mechanistic exploration. Here, we hypothesize a four-oscillator model that controls the bimodal rhythms. The four oscillators that reside in different clock neurons regulate activity in the morning and evening and sleep during the midday and at night. In this way, bimodal rhythms are formed by interactions among the four oscillators (two activity and two sleep oscillators), which may judiciously explain the flexible waveform of activity rhythms under different photoperiod conditions. Although still hypothetical, this model would provide a new perspective on the seasonal adaptation of the two activity peaks.

    DOI: 10.1007/s00359-023-01639-5

    PubMed

    researchmap

  • Pigment-dispersing factor and CCHamide1 in the Drosophila circadian clock network. Reviewed International journal

    Riko Kuwano, Maki Katsura, Mai Iwata, Tatsuya Yokosako, Taishi Yoshii

    Chronobiology international   1 - 16   2023.2

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Animals possess a circadian central clock in the brain, where circadian behavioural rhythms are generated. In the fruit fly (Drosophila melanogaster), the central clock comprises a network of approximately 150 clock neurons, which is important for the maintenance of a coherent and robust rhythm. Several neuropeptides involved in the network have been identified, including Pigment-dispersing factor (PDF) and CCHamide1 (CCHa1) neuropeptides. PDF signals bidirectionally to CCHa1-positive clock neurons; thus, the clock neuron groups expressing PDF and CCHa1 interact reciprocally. However, the role of these interactions in molecular and behavioural rhythms remains elusive. In this study, we generated Pdf 01 and CCHa1SK8 double mutants and examined their locomotor activity-related rhythms. The single mutants of Pdf 01 or CCHa1SK8 displayed free-running rhythms under constant dark conditions, whereas approximately 98% of the double mutants were arrhythmic. In light-dark conditions, the evening activity of the double mutants was phase-advanced compared with that of the single mutants. In contrast, both the single and double mutants had diminished morning activity. These results suggest that the effects of the double mutation varied in behavioural parameters. The double and triple mutants of per 01, Pdf 01, and CCHa1SK8 further revealed that PDF signalling plays a role in the suppression of activity during the daytime under a clock-less background. Our results provide insights into the interactions between PDF and CCHa1 signalling and their roles in activity rhythms.

    DOI: 10.1080/07420528.2023.2166416

    PubMed

    researchmap

  • Timeless Plays an Important Role in Compound Eye-Dependent Photic Entrainment of the Circadian Rhythm in the Cricket Gryllus bimaculatus Reviewed

    Yoshiyuki Moriyama, Kazuki Takeuchi, Tsugumichi Shinohara, Koichi Miyagawa, Mirai Matsuka, Taishi Yoshii, Kenji Tomioka

    Zoological Science   39 ( 4 )   2022.6

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Zoological Society of Japan  

    DOI: 10.2108/zs220011

    researchmap

  • The lateral posterior clock neurons of Drosophila melanogaster express three neuropeptides and have multiple connections within the circadian clock network and beyond. Reviewed International coauthorship International journal

    Nils Reinhard, Enrico Bertolini, Aika Saito, Manabu Sekiguchi, Taishi Yoshii, Dirk Rieger, Charlotte Helfrich-Förster

    The Journal of comparative neurology   530 ( 9 )   1507 - 1529   2022.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Drosophila's lateral posterior neurons (LPNs) belong to a small group of circadian clock neurons that is so far not characterized in detail. Thanks to a new highly specific split-Gal4 line, here we describe LPNs' morphology in fine detail, their synaptic connections, daily bimodal expression of neuropeptides, and propose a putative role of this cluster in controlling daily activity and sleep patterns. We found that the three LPNs are heterogeneous. Two of the neurons with similar morphology arborize in the superior medial and lateral protocerebrum and most likely promote sleep. One unique, possibly wakefulness-promoting, neuron with wider arborizations extends from the superior lateral protocerebrum toward the anterior optic tubercle. Both LPN types exhibit manifold connections with the other circadian clock neurons, especially with those that control the flies' morning and evening activity (M- and E-neurons, respectively). In addition, they form synaptic connections with neurons of the mushroom bodies, the fan-shaped body, and with many additional still unidentified neurons. We found that both LPN types rhythmically express three neuropeptides, Allostatin A, Allostatin C, and Diuretic Hormone 31 with maxima in the morning and the evening. The three LPN neuropeptides may, furthermore, signal to the insect hormonal center in the pars intercerebralis and contribute to rhythmic modulation of metabolism, feeding, and reproduction. We discuss our findings in the light of anatomical details gained by the recently published hemibrain of a single female fly on the electron microscopic level and of previous functional studies concerning the LPN.

    DOI: 10.1002/cne.25294

    PubMed

    researchmap

  • Dorsal clock networks drive temperature preference rhythms in Drosophila. Reviewed International coauthorship International journal

    Shyh-Chi Chen, Xin Tang, Tadahiro Goda, Yujiro Umezaki, Abigail C Riley, Manabu Sekiguchi, Taishi Yoshii, Fumika N Hamada

    Cell reports   39 ( 2 )   110668 - 110668   2022.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Animals display a body temperature rhythm (BTR). Little is known about the mechanisms by which a rhythmic pattern of BTR is regulated and how body temperature is set at different times of the day. As small ectotherms, Drosophila exhibit a daily temperature preference rhythm (TPR), which generates BTR. Here, we demonstrate dorsal clock networks that play essential roles in TPR. Dorsal neurons 2 (DN2s) are the main clock for TPR. We find that DN2s and posterior DN1s (DN1ps) contact and the extent of contacts increases during the day and that the silencing of DN2s or DN1ps leads to a lower temperature preference. The data suggest that temporal control of the microcircuit from DN2s to DN1ps contributes to TPR regulation. We also identify anterior DN1s (DN1as) as another important clock for TPR. Thus, we show that the DN networks predominantly control TPR and determine both a rhythmic pattern and preferred temperatures.

    DOI: 10.1016/j.celrep.2022.110668

    PubMed

    researchmap

  • Artificial selections for death‐feigning behavior in beetles show correlated responses in amplitude of circadian rhythms, but the period of the rhythm does not Reviewed International coauthorship

    Takahisa Miyatake, Masato S. Abe, Kentarou Matsumura, Taishi Yoshii

    Ethology   2022.3

     More details

    Authorship:Last author   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/eth.13279

    researchmap

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/eth.13279

  • The Neuronal Circuit of the Dorsal Circadian Clock Neurons in Drosophila melanogaster. Reviewed International coauthorship International journal

    Nils Reinhard, Frank K Schubert, Enrico Bertolini, Nicolas Hagedorn, Giulia Manoli, Manabu Sekiguchi, Taishi Yoshii, Dirk Rieger, Charlotte Helfrich-Förster

    Frontiers in physiology   13   886432 - 886432   2022

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Drosophila's dorsal clock neurons (DNs) consist of four clusters (DN1as, DN1ps, DN2s, and DN3s) that largely differ in size. While the DN1as and the DN2s encompass only two neurons, the DN1ps consist of ∼15 neurons, and the DN3s comprise ∼40 neurons per brain hemisphere. In comparison to the well-characterized lateral clock neurons (LNs), the neuroanatomy and function of the DNs are still not clear. Over the past decade, numerous studies have addressed their role in the fly's circadian system, leading to several sometimes divergent results. Nonetheless, these studies agreed that the DNs are important to fine-tune activity under light and temperature cycles and play essential roles in linking the output from the LNs to downstream neurons that control sleep and metabolism. Here, we used the Flybow system, specific split-GAL4 lines, trans-Tango, and the recently published fly connectome (called hemibrain) to describe the morphology of the DNs in greater detail, including their synaptic connections to other clock and non-clock neurons. We show that some DN groups are largely heterogenous. While certain DNs are strongly connected with the LNs, others are mainly output neurons that signal to circuits downstream of the clock. Among the latter are mushroom body neurons, central complex neurons, tubercle bulb neurons, neurosecretory cells in the pars intercerebralis, and other still unidentified partners. This heterogeneity of the DNs may explain some of the conflicting results previously found about their functionality. Most importantly, we identify two putative novel communication centers of the clock network: one fiber bundle in the superior lateral protocerebrum running toward the anterior optic tubercle and one fiber hub in the posterior lateral protocerebrum. Both are invaded by several DNs and LNs and might play an instrumental role in the clock network.

    DOI: 10.3389/fphys.2022.886432

    PubMed

    researchmap

  • Decapentaplegic Acutely Defines the Connectivity of Central Pacemaker Neurons in Drosophila. Reviewed International coauthorship International journal

    Sofía Polcowñuk, Taishi Yoshii, M Fernanda Ceriani

    The Journal of neuroscience : the official journal of the Society for Neuroscience   41 ( 40 )   8338 - 8350   2021.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Rhythmic rest-activity cycles are controlled by an endogenous clock. In Drosophila, this clock resides in ∼150 neurons organized in clusters whose hierarchy changes in response to environmental conditions. The concerted activity of the circadian network is necessary for the adaptive responses to synchronizing environmental stimuli. Thus far, work was devoted to unravel the logic of the coordination of different clusters focusing on neurotransmitters and neuropeptides. We further explored communication in the adult male brain through ligands belonging to the bone morphogenetic protein (BMP) pathway. Herein we show that the lateral ventral neurons (LNvs) express the small morphogen decapentaplegic (DPP). DPP expression in the large LNvs triggered a period lengthening phenotype, the downregulation of which caused reduced rhythmicity and affected anticipation at dawn and dusk, underscoring DPP per se conveys time-of-day relevant information. Surprisingly, DPP expression in the large LNvs impaired circadian remodeling of the small LNv axonal terminals, likely through local modulation of the guanine nucleotide exchange factor Trio. These findings open the provocative possibility that the BMP pathway is recruited to strengthen/reduce the connectivity among specific clusters along the day and thus modulate the contribution of the clusters to the circadian network.SIGNIFICANCE STATEMENT The circadian clock relies on the communication between groups of so-called clock neurons to coordinate physiology and behavior to the optimal times across the day, predicting and adapting to a changing environment. The circadian network relies on neurotransmitters and neuropeptides to fine-tune connectivity among clock neurons and thus give rise to a coherent output. Herein we show that decapentaplegic, a ligand belonging to the BMP retrograde signaling pathway required for coordinated growth during development, is recruited by a group of circadian neurons in the adult brain to trigger structural remodeling of terminals on a daily basis.

    DOI: 10.1523/JNEUROSCI.0397-21.2021

    PubMed

    researchmap

  • Antibodies Against the Clock Proteins Period and Cryptochrome Reveal the Neuronal Organization of the Circadian Clock in the Pea Aphid. Reviewed International coauthorship International journal

    Francesca Sara Colizzi, Katharina Beer, Paolo Cuti, Peter Deppisch, David Martínez Torres, Taishi Yoshii, Charlotte Helfrich-Förster

    Frontiers in physiology   12   705048 - 705048   2021

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Circadian clocks prepare the organism to cyclic environmental changes in light, temperature, or food availability. Here, we characterized the master clock in the brain of a strongly photoperiodic insect, the aphid Acyrthosiphon pisum, immunohistochemically with antibodies against A. pisum Period (PER), Drosophila melanogaster Cryptochrome (CRY1), and crab Pigment-Dispersing Hormone (PDH). The latter antibody detects all so far known PDHs and PDFs (Pigment-Dispersing Factors), which play a dominant role in the circadian system of many arthropods. We found that, under long days, PER and CRY are expressed in a rhythmic manner in three regions of the brain: the dorsal and lateral protocerebrum and the lamina. No staining was detected with anti-PDH, suggesting that aphids lack PDF. All the CRY1-positive cells co-expressed PER and showed daily PER/CRY1 oscillations of high amplitude, while the PER oscillations of the CRY1-negative PER neurons were of considerable lower amplitude. The CRY1 oscillations were highly synchronous in all neurons, suggesting that aphid CRY1, similarly to Drosophila CRY1, is light sensitive and its oscillations are synchronized by light-dark cycles. Nevertheless, in contrast to Drosophila CRY1, aphid CRY1 was not degraded by light, but steadily increased during the day and decreased during the night. PER was always located in the nuclei of the clock neurons, while CRY was predominantly cytoplasmic and revealed the projections of the PER/CRY1-positive neurons. We traced the PER/CRY1-positive neurons through the aphid protocerebrum discovering striking similarities with the circadian clock of D. melanogaster: The CRY1 fibers innervate the dorsal and lateral protocerebrum and putatively connect the different PER-positive neurons with each other. They also run toward the pars intercerebralis, which controls hormone release via the neurohemal organ, the corpora cardiaca. In contrast to Drosophila, the CRY1-positive fibers additionally travel directly toward the corpora cardiaca and the close-by endocrine gland, corpora allata. This suggests a direct link between the circadian clock and the photoperiodic control of hormone release that can be studied in the future.

    DOI: 10.3389/fphys.2021.705048

    PubMed

    researchmap

  • Amplitude of circadian rhythms becomes weaken in the north, but there is no cline in the period of rhythm in a beetle. Reviewed International journal

    Masato S Abe, Kentarou Matsumura, Taishi Yoshii, Takahisa Miyatake

    PloS one   16 ( 1 )   e0245115   2021

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Many species show rhythmicity in activity, from the timing of flowering in plants to that of foraging behavior in animals. The free-running periods and amplitude (sometimes called strength or power) of circadian rhythms are often used as indicators of biological clocks. Many reports have shown that these traits are highly geographically variable, and interestingly, they often show latitudinal or longitudinal clines. In many cases, the higher the latitude is, the longer the free-running circadian period (i.e., period of rhythm) in insects and plants. However, reports of positive correlations between latitude or longitude and circadian rhythm traits, including free-running periods, the power of the rhythm and locomotor activity, are limited to certain taxonomic groups. Therefore, we collected a cosmopolitan stored-product pest species, the red flour beetle Tribolium castaneum, in various parts of Japan and examined its rhythm traits, including the power and period of the rhythm, which were calculated from locomotor activity. The analysis revealed that the power was significantly lower for beetles collected in northern areas than southern areas in Japan. However, it is worth noting that the period of circadian rhythm did not show any clines; specifically, it did not vary among the sampling sites, despite the very large sample size (n = 1585). We discuss why these cline trends were observed in T. castaneum.

    DOI: 10.1371/journal.pone.0245115

    PubMed

    researchmap

  • Dopamine Signaling in Wake-Promoting Clock Neurons Is Not Required for the Normal Regulation of Sleep in Drosophila Reviewed

    Florencia Fernandez-Chiappe, Christiane Hermann-Luibl, Alina Peteranderl, Nils Reinhard, Pingkalai R. Senthilan, Marie Hieke, Mareike Selcho, Taishi Yoshii, Orie T. Shafer, Nara I. Muraro, Charlotte Helfrich-Förster

    The Journal of Neuroscience   40 ( 50 )   9617 - 9633   2020.12

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Society for Neuroscience  

    DOI: 10.1523/jneurosci.1488-20.2020

    researchmap

  • Coupling Neuropeptide Levels to Structural Plasticity in Drosophila Clock Neurons. Reviewed International journal

    Anastasia Herrero, Taishi Yoshii, Juan Ignacio Ispizua, Carina Colque, Jan A Veenstra, Nara I Muraro, María Fernanda Ceriani

    Current biology : CB   2020.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We have previously reported that pigment dispersing factor (PDF) neurons, which are essential in the control of rest-activity cycles in Drosophila, undergo circadian remodeling of their axonal projections, a phenomenon called circadian structural plasticity. Axonal arborizations display higher complexity during the day and become simpler at night, and this remodeling involves changes in the degree of connectivity. This phenomenon depends on the clock present within the ventrolateral neurons (LNvs) as well as in glia. In this work, we characterize in detail the contribution of the PDF neuropeptide to structural plasticity at different times across the day. Using diverse genetic strategies to temporally restrict its downregulation, we demonstrate that even subtle alterations to PDF cycling at the dorsal protocerebrum correlate with impaired remodeling, underscoring its relevance for the characteristic morning spread; PDF released from the small LNvs (sLNvs) and the large LNvs (lLNvs) contribute to the process. Moreover, forced depolarization recruits activity-dependent mechanisms to mediate growth only at night, overcoming the restriction imposed by the clock on membrane excitability. Interestingly, the active process of terminal remodeling requires PDF receptor (PDFR) signaling acting locally through the cyclic-nucleotide-gated channel ion channel subunit A (CNGA). Thus, clock-dependent PDF signaling shapes the connectivity of these essential clock neurons on daily basis.

    DOI: 10.1016/j.cub.2020.06.009

    PubMed

    researchmap

  • Genetic variation and phenotypic plasticity in circadian rhythms in an armed beetle, Gnatocerus cornutus (Tenebrionidae) Reviewed

    Kentarou Matsumura, Masato S Abe, Manmohan D Sharma, David J Hosken, Taishi Yoshii, Takahisa Miyatake

    Biological Journal of the Linnean Society   130 ( 1 )   34 - 40   2020.5

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    <title>Abstract</title>
    Circadian rhythms, their free-running periods and the power of the rhythms are often used as indicators of biological clocks, and there is evidence that the free-running periods of circadian rhythms are not affected by environmental factors, such as temperature. However, there are few studies of environmental effects on the power of the rhythms, and it is not clear whether temperature compensation is universal. Additionally, genetic variation and phenotypic plasticity in biological clocks are important for understanding the evolution of biological rhythms, but genetic and plastic effects are rarely investigated. Here, we used 18 isofemale lines (genotypes) of Gnatocerus cornutus to assess rhythms of locomotor activity, while also testing for temperature effects. We found that total activity and the power of the circadian rhythm were affected by interactions between sex and genotype or between sex, genotype and temperature. The males tended to be more active and showed greater increases in activity, but this effect varied across both genotypes and temperatures. The period of activity varied only by genotype and was thus independent of temperature. The complicated genotype–sex–environment interactions we recorded stress the importance of investigating circadian activity in more integrated ways.

    DOI: 10.1093/biolinnean/blaa016

    researchmap

  • A Catalog of GAL4 Drivers for Labeling and Manipulating Circadian Clock Neurons in Drosophila melanogaster. Reviewed International journal

    Manabu Sekiguchi, Kotaro Inoue, Tian Yang, Dong-Gen Luo, Taishi Yoshii

    Journal of biological rhythms   35 ( 2 )   207 - 213   2020.4

     More details

    Authorship:Last author, Corresponding author   Language:English  

    Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly's brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.

    DOI: 10.1177/0748730419895154

    PubMed

    researchmap

  • The circadian clock improves fitness in the fruit fly, Drosophila melanogaster. Reviewed

    Horn M, Mitesser O, Hovestadt T, Yoshii T, Rieger D, Helfrich-Förster C

    Frontiers in Physiology   10   1374   2019.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    researchmap

  • Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in Drosophila Reviewed

    Li MT, Cao LH, Xiao N, Tang M, Deng B, Yang T, Yoshii T, Luo DG

    Nature Communications   9 ( 1 )   2018.12

     More details

    Publisher:Springer Nature America, Inc  

    DOI: 10.1038/s41467-018-06506-5

    researchmap

  • Neuroanatomical details of the lateral neurons of Drosophila melanogaster support their functional role in the circadian system Reviewed

    Frank K. Schubert, Nicolas Hagedorn, Taishi Yoshii, Charlotte Helfrich-Förster, Dirk Rieger

    Journal of Comparative Neurology   526 ( 7 )   1209 - 1231   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley-Liss Inc.  

    DOI: 10.1002/cne.24406

    Scopus

    researchmap

    Other Link: http://orcid.org/0000-0002-7057-7986

  • A Tug-of-War between Cryptochrome and the Visual System Allows the Adaptation of Evening Activity to Long Photoperiods in Drosophila melanogaster Reviewed

    Christa Kistenpfennig, Mayumi Nakayama, Ruri Nihara, Kenji Tomioka, Charlotte Helfrich-Förster, Taishi Yoshii

    Journal of Biological Rhythms   33 ( 1 )   24 - 34   2018.2

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SAGE Publications Inc.  

    DOI: 10.1177/0748730417738612

    Scopus

    researchmap

    Other Link: http://orcid.org/0000-0002-7057-7986

  • The CCHamide1 Neuropeptide Expressed in the Anterior Dorsal Neuron 1 Conveys a Circadian Signal to the Ventral Lateral Neurons in Drosophila melanogaster. Reviewed International journal

    Fujiwara Y, Hermann-Luibl C, Katsura M, Sekiguchi M, Ida T, Helfrich-Förster C, Yoshii T

    Frontiers in physiology   9   1276 - 1276   2018

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    The fruit fly Drosophila melanogaster possesses approximately 150 brain clock neurons that control circadian behavioral rhythms. Even though individual clock neurons have self-sustaining oscillators, they interact and synchronize with each other through a network. However, little is known regarding the factors responsible for these network interactions. In this study, we investigated the role of CCHamide1 (CCHa1), a neuropeptide expressed in the anterior dorsal neuron 1 (DN1a), in intercellular communication of the clock neurons. We observed that CCHa1 connects the DN1a clock neurons to the ventral lateral clock neurons (LNv) via the CCHa1 receptor, which is a homolog of the gastrin-releasing peptide receptor playing a role in circadian intercellular communications in mammals. CCHa1 knockout or knockdown flies have a generally low activity level with a special reduction of morning activity. In addition, they exhibit advanced morning activity under light-dark cycles and delayed activity under constant dark conditions, which correlates with an advance/delay of PAR domain Protein 1 (PDP1) oscillations in the small-LNv (s-LNv) neurons that control morning activity. The terminals of the s-LNv neurons show rather high levels of Pigment-dispersing factor (PDF) in the evening, when PDF is low in control flies, suggesting that the knockdown of CCHa1 leads to increased PDF release; PDF signals the other clock neurons and evidently increases the amplitude of their PDP1 cycling. A previous study showed that high-amplitude PDP1 cycling increases the siesta of the flies, and indeed, CCHa1 knockout or knockdown flies exhibit a longer siesta than control flies. The DN1a neurons are known to be receptive to PDF signaling from the s-LNv neurons; thus, our results suggest that the DN1a and s-LNv clock neurons are reciprocally coupled via the neuropeptides CCHa1 and PDF, and this interaction fine-tunes the timing of activity and sleep.

    DOI: 10.3389/fphys.2018.01276

    PubMed

    researchmap

  • Circadian light-input pathways in Drosophila Reviewed

    Taishi Yoshii, Christiane Hermann-Luibl, Charlotte Helfrich-Föorster

    Communicative and Integrative Biology   9 ( 1 )   2016

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publisher:Taylor and Francis Inc.  

    DOI: 10.1080/19420889.2015.1102805

    Scopus

    researchmap

    Other Link: http://orcid.org/0000-0002-7057-7986

  • Cryptochrome-dependent and -independent circadian entrainment circuits in Drosophila. Reviewed International journal

    Taishi Yoshii, Christiane Hermann-Luibl, Christa Kistenpfennig, Benjamin Schmid, Kenji Tomioka, Charlotte Helfrich-Förster

    The Journal of neuroscience : the official journal of the Society for Neuroscience   35 ( 15 )   6131 - 41   2015.4

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Entrainment to environmental light/dark (LD) cycles is a central function of circadian clocks. In Drosophila, entrainment is achieved by Cryptochrome (CRY) and input from the visual system. During activation by brief light pulses, CRY triggers the degradation of TIMELESS and subsequent shift in circadian phase. This is less important for LD entrainment, leading to questions regarding light input circuits and mechanisms from the visual system. Recent studies show that different subsets of brain pacemaker clock neurons, the morning (M) and evening (E) oscillators, have distinct functions in light entrainment. However, the role of CRY in M and E oscillators for entrainment to LD cycles is unknown. Here, we address this question by selectively expressing CRY in different subsets of clock neurons in a cry-null (cry(0)) mutant background. We were able to rescue the light entrainment deficits of cry(0) mutants by expressing CRY in E oscillators but not in any other clock neurons. Par domain protein 1 molecular oscillations in the E, but not M, cells of cry(0) mutants still responded to the LD phase delay. This residual light response was stemming from the visual system because it disappeared when all external photoreceptors were ablated genetically. We concluded that the E oscillators are the targets of light input via CRY and the visual system and are required for normal light entrainment.

    DOI: 10.1523/JNEUROSCI.0070-15.2015

    PubMed

    researchmap

  • Suppressive effects of dRYamides on feeding behavior of the blowfly, Phormia regina. Reviewed International journal

    Maeda T, Nakamura Y, Shiotani H, Hojo MK, Yoshii T, Ida T, Sato T, Yoshida M, Miyazato M, Kojima M, Ozaki M

    Zoological letters   1   35 - 35   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Recently, dRYamides-1 and -2 have been identified as ligands of the neuropeptide Y-like receptor CG5811 in Drosophila melanogaster. It has also been reported in brief that injection of dRYamide-1suppresses the early feeding behavior called proboscis extension reflex (PER) in the blowfly Phormia regina. Immunohistochemical analyses by our group using anti-dRYamide-1 antiserum indicated symmetrical localization of 32 immunoreactive cells in the brain of P. regina. In order to analyze the mechanism of feeding regulation, we further investigated the effects of dRYamide-1 and -2 on intake volume, PER exhibition, and activity of the sugar receptor neuron. After injection of dRYamide-1 or -2, flies showed little change in the intake volume of sucrose solution, but a significant depression of PER to sucrose. Injection of dRYamide-1 revealed a significant decrease in the responsiveness of the sugar receptor neuron, although the injection of dRYamide-2 did not. These results suggest that the dRYamide peptides decrease feeding motivation in flies, as evaluated by PER threshold, through a mechanism that partially involves desensitization of the sugar receptor neuron.

    DOI: 10.1186/s40851-015-0034-z

    PubMed

    researchmap

  • Green-sensitive opsin is the photoreceptor for photic entrainment of an insect circadian clock. Reviewed

    Komada S, Kamae Y, Koyanagi M, Tatewaki K, Hassaneen E, Saifullah A, Yoshii T, Terakita A, Tomioka K

    Zoological letters   1   11   2015

  • The MAP Kinase p38 Is Part of Drosophila melanogaster's Circadian Clock Reviewed

    Verena Dusik, Pingkalai R. Senthilan, Benjamin Mentzel, Heiko Hartlieb, Corinna Wuelbeck, Taishi Yoshii, Thomas Raabe, Charlotte Helfrich-Foerster

    PLOS GENETICS   10 ( 8 )   2014.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pgen.1004565

    Web of Science

    researchmap

  • The ion transport peptide is a new functional clock neuropeptide in the fruit fly Drosophila melanogaster. Reviewed International journal

    Christiane Hermann-Luibl, Taishi Yoshii, Pingkalai R Senthilan, Heinrich Dircksen, Charlotte Helfrich-Förster

    The Journal of neuroscience : the official journal of the Society for Neuroscience   34 ( 29 )   9522 - 36   2014.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The clock network of Drosophila melanogaster expresses various neuropeptides, but a function in clock-mediated behavioral control was so far only found for the neuropeptide pigment dispersing factor (PDF). Here, we propose a role in the control of behavioral rhythms for the ion transport peptide (ITP), which is expressed in the fifth small ventral lateral neuron, one dorsal lateral neuron, and in only a few nonclock cells in the brain. Immunocytochemical analyses revealed that ITP, like PDF, is most probably released in a rhythmic manner at projection terminals in the dorsal protocerebrum. This rhythm continues under constant dark conditions, indicating that ITP release is clock controlled. ITP expression is reduced in the hypomorph mutant Clk(AR), suggesting that ITP expression is regulated by CLOCK. Using a genetically encoded RNAi construct, we knocked down ITP in the two clock cells and found that these flies show reduced evening activity and increased nocturnal activity. Overexpression of ITP with two independent timeless-GAL4 lines completely disrupted behavioral rhythms, but only slightly dampened PER cycling in important pacemaker neurons, suggesting a role for ITP in clock output pathways rather than in the communication within the clock network. Simultaneous knockdown (KD) of ITP and PDF made the flies hyperactive and almost completely arrhythmic under constant conditions. Under light-dark conditions, the double-KD combined the behavioral characteristics of the single-KD flies. In addition, it reduced the flies' sleep. We conclude that ITP and PDF are the clock's main output signals that cooperate in controlling the flies' activity rhythms.

    DOI: 10.1523/JNEUROSCI.0111-14.2014

    PubMed

    researchmap

  • Moonlight Detection by Drosophila's Endogenous Clock Depends on Multiple Photopigments in the Compound Eyes Reviewed

    Matthias Schlichting, Rudi Grebler, Nicolai Peschel, Taishi Yoshii, Charlotte Helfrich-Foerster

    JOURNAL OF BIOLOGICAL RHYTHMS   29 ( 2 )   75 - 86   2014.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730413520428

    Web of Science

    researchmap

  • Sexual Interactions Influence the Molecular Oscillations in DN1 Pacemaker Neurons in Drosophila melanogaster Reviewed

    Shiho Hanafusa, Tomoaki Kawaguchi, Yujiro Umezaki, Kenji Tomioka, Taishi Yoshii

    PLOS ONE   8 ( 12 )   2013.12

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0084495

    Web of Science

    researchmap

  • GABAB receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster Reviewed

    Florian Gmeiner, Agata Kolodziejczyk, Taishi Yoshii, Dirk Rieger, Dick R. Nässel, Charlotte Helfrich-Förster

    Journal of Experimental Biology   216 ( 20 )   3837 - 3843   2013.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jeb.085563

    Web of Science

    Scopus

    PubMed

    researchmap

  • Chronic electromyographic analysis of circadian locomotor activity in crayfish Reviewed

    Yusuke Tomina, Akihiro Kibayashi, Taishi Yoshii, Masakazu Takahata

    Behavioural Brain Research   249   90 - 103   2013.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbr.2013.04.029

    Web of Science

    Scopus

    PubMed

    researchmap

  • Exquisite Light Sensitivity of Drosophila melanogaster Cryptochrome Reviewed

    Pooja Vinayak, Jamie Coupar, S. Emile Hughes, Preeya Fozdar, Jack Kilby, Emma Garren, Taishi Yoshii, Jay Hirsh

    PLOS GENETICS   9 ( 7 )   2013.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pgen.1003615

    Web of Science

    researchmap

  • Long-term effect of systemic RNA interference on circadian clock genes in hemimetabolous insects Reviewed

    Outa Uryu, Yuichi Kamae, Kenji Tomioka, Taishi Yoshii

    JOURNAL OF INSECT PHYSIOLOGY   59 ( 4 )   494 - 499   2013.4

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jinsphys.2013.02.009

    Web of Science

    researchmap

  • The circadian clock network in the brain of different Drosophila species Reviewed

    Christiane Hermann, Rachele Saccon, Pingkalai R. Senthilan, Lilith Domnik, Heinrich Dircksen, Taishi Yoshii, Charlotte Helfrich-Förster

    Journal of Comparative Neurology   521 ( 2 )   367 - 388   2013.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cne.23178

    Web of Science

    Scopus

    PubMed

    researchmap

  • Drosophila Clock Neurons under Natural Conditions Reviewed

    Pamela Menegazzi, Stefano Vanin, Taishi Yoshii, Dirk Rieger, Christiane Hermann, Verena Dusik, Charalambos P. Kyriacou, Charlotte Helfrich-Foerster, Rodolfo Costa

    JOURNAL OF BIOLOGICAL RHYTHMS   28 ( 1 )   3 - 14   2013.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730412471303

    Web of Science

    researchmap

  • Laboratory versus Nature: The Two Sides of the Drosophila Circadian Clock Reviewed

    Pamela Menegazzi, Taishi Yoshii, Charlotte Helfrich-Foerster

    JOURNAL OF BIOLOGICAL RHYTHMS   27 ( 6 )   433 - 442   2012.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730412463181

    Web of Science

    researchmap

  • Pigment-Dispersing Factor Is Involved in Age-Dependent Rhythm Changes in Drosophila melanogaster Reviewed

    Yujiro Umezaki, Taishi Yoshii, Tomoaki Kawaguchi, Charlotte Helfrich-Foerster, Kenji Tomioka

    JOURNAL OF BIOLOGICAL RHYTHMS   27 ( 6 )   423 - 432   2012.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730412462206

    Web of Science

    researchmap

  • Peripheral circadian rhythms and their regulatory mechanism in insects and some other arthropods: a review Reviewed

    Kenji Tomioka, Outa Uryu, Yuichi Kamae, Yujiro Umezaki, Taishi Yoshii

    JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY   182 ( 6 )   729 - 740   2012.8

     More details

  • Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free-running period in Drosophila melanogaster Reviewed

    Christiane Hermann, Taishi Yoshii, Verena Dusik, Charlotte Helfrich-Foerster

    JOURNAL OF COMPARATIVE NEUROLOGY   520 ( 5 )   970 - 987   2012.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cne.22742

    Web of Science

    researchmap

  • Phase-Shifting the Fruit Fly Clock without Cryptochrome Reviewed

    Christa Kistenpfennig, Jay Hirsh, Taishi Yoshii, Charlotte Helfrich-Foerster

    JOURNAL OF BIOLOGICAL RHYTHMS   27 ( 2 )   117 - 125   2012.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730411434390

    Web of Science

    researchmap

  • Human Cryptochrome-1 Confers Light Independent Biological Activity in Transgenic Drosophila Correlated with Flavin Radical Stability Reviewed

    Jacqueline Vieira, Alex R. Jones, Antoine Danon, Michiyo Sakuma, Nathalie Hoang, David Robles, Shirley Tait, Derren J. Heyes, Marie Picot, Taishi Yoshii, Charlotte Helfrich-Foerster, Guillaume Soubigou, Jean-Yves Coppee, Andre Klarsfeld, Francois Rouyer, Nigel S. Scrutton, Margaret Ahmad

    PLOS ONE   7 ( 3 )   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0031867

    Web of Science

    researchmap

  • The Dual-Oscillator System of Drosophila melanogaster Under Natural-Like Temperature Cycles Reviewed

    Wolfgang Bywalez, Pamela Menegazzi, Dirk Rieger, Benjamin Schmid, Charlotte Helfrich-Foerster, Taishi Yoshii

    CHRONOBIOLOGY INTERNATIONAL   29 ( 4 )   395 - 407   2012

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3109/07420528.2012.668505

    Web of Science

    researchmap

  • Two clocks in the brain: An update of the morning and evening oscillator model in Drosophila Reviewed

    Taishi Yoshii, Dirk Rieger, Charlotte Helfrich-Foerster

    NEUROBIOLOGY OF CIRCADIAN TIMING   199   59 - 82   2012

     More details

    Authorship:Lead author   Language:English   Publishing type:Part of collection (book)  

    DOI: 10.1016/B978-0-444-59427-3.00027-7

    Web of Science

    researchmap

  • A New ImageJ Plug-in "ActogramJ" for Chronobiological Analyses Reviewed

    Benjamin Schmid, Charlotte Helfrich-Foerster, Taishi Yoshii

    JOURNAL OF BIOLOGICAL RHYTHMS   26 ( 5 )   464 - 467   2011.10

     More details

    Authorship:Last author, Corresponding author   Language:English  

    DOI: 10.1177/0748730411414264

    Web of Science

    researchmap

  • Cryptochrome-Positive and -Negative Clock Neurons in Drosophila Entrain Differentially to Light and Temperature Reviewed

    Taishi Yoshii, Christiane Hermann, Charlotte Helfrich-Forster

    JOURNAL OF BIOLOGICAL RHYTHMS   25 ( 6 )   387 - 398   2010.12

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730410381962

    Web of Science

    researchmap

  • Cryptochrome - a photoreceptor with the properties of a magnetoreceptor? Reviewed

    Ritz T, Yoshii T, Helfrich-Förster C, Ahmad M

    Communicative & Integrative Biology   2010

     More details

    Language:English  

    DOI: 10.4161/cib.3.1.10300

    researchmap

  • Synergic Entrainment of Drosophila&apos;s Circadian Clock by Light and Temperature Reviewed

    Taishi Yoshii, Stefano Vanin, Rodolfo Costa, Charlotte Helfrich-Foerster

    JOURNAL OF BIOLOGICAL RHYTHMS   24 ( 6 )   452 - 464   2009.12

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730409348551

    Web of Science

    researchmap

  • Peptidergic Clock Neurons in Drosophila: Ion Transport Peptide and Short Neuropeptide F in Subsets of Dorsal and Ventral Lateral Neurons Reviewed

    Helena A. D. Johard, Taishi Yoishii, Heinrich Dircksen, Paola Cusumano, Francois Rouyer, Charlotte Helfrich-Foerster, Dick R. Nassel

    JOURNAL OF COMPARATIVE NEUROLOGY   516 ( 1 )   59 - 73   2009.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cne.22099

    Web of Science

    researchmap

  • Cryptochrome Mediates Light-Dependent Magnetosensitivity of Drosophila's Circadian Clock Reviewed

    Taishi Yoshii, Margaret Ahmad, Charlotte Helfrich-Foerster

    PLOS BIOLOGY   7 ( 4 )   813 - 819   2009.4

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pbio.1000086

    Web of Science

    researchmap

  • The Neuropeptide Pigment-Dispersing Factor Adjusts Period and Phase of Drosophila's Clock Reviewed

    Taishi Yoshii, Corinna Wuelbeck, Hana Sehadova, Shobi Veleri, Dominik Bichler, Ralf Stanewsky, Charlotte Helfrich-Foerster

    JOURNAL OF NEUROSCIENCE   29 ( 8 )   2597 - 2610   2009.2

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1523/JNEUROSCI.5439-08.2009

    Web of Science

    researchmap

  • Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons Reviewed

    Taishi Yoshii, Takeshi Todo, Corinna Wuelbeck, Ralf Stanewsky, Charlotte Helfrich-Foerster

    JOURNAL OF COMPARATIVE NEUROLOGY   508 ( 6 )   952 - 966   2008.6

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cne.21702

    Web of Science

    researchmap

  • Induction of Drosophila behavioral and molecular circadian rhythms by temperature steps in constant light Reviewed

    Taishi Yoshii, Kana Fujii, Kenji Tomioka

    JOURNAL OF BIOLOGICAL RHYTHMS   22 ( 2 )   103 - 114   2007.4

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0748730406298176

    Web of Science

    researchmap

  • The lateral and dorsal neurons of Drosophila melanogaster: New insights about their morphology and function Reviewed

    C. Helfrich-Foerster, T. Yoshii, C. Wuelbeck, E. Grieshaber, D. Rieger, W. Bachleitner, P. Cusumano, F. Rouyer

    COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY   72   517 - 525   2007

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

    DOI: 10.1101/sqb.2007.72.063

    Web of Science

    researchmap

  • Entrainment of Drosophila circadian rhythms by temperature cycles Reviewed

    Kenji Tomioka, Taishi Yoshii

    Sleep and Biological Rhythms   4 ( 3 )   240 - 247   2006.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1479-8425.2006.00227.x

    Scopus

    researchmap

  • Temperature cycles drive Drosophila circadian oscillation in constant light that otherwise induces behavioural arrhythmicity Reviewed

    T Yoshii, Y Heshiki, T Ibuki-Ishibashi, A Matsumoto, T Tanimura, K Tomioka

    EUROPEAN JOURNAL OF NEUROSCIENCE   22 ( 5 )   1176 - 1184   2005.9

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1460-9568.2005.04295.x

    Web of Science

    researchmap

  • Drosophila cry(b) mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light Reviewed

    T Yoshii, Y Funada, T Ibuki-Ishibashi, A Matsumoto, T Tanimura, K Tomioka

    JOURNAL OF INSECT PHYSIOLOGY   50 ( 6 )   479 - 488   2004.6

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jinsphys.2004.02.011

    Web of Science

    researchmap

  • A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster Reviewed

    T Yoshii, M Sakamoto, K Tomioka

    ZOOLOGICAL SCIENCE   19 ( 8 )   841 - 850   2002.8

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2108/zsj.19.841

    Web of Science

    researchmap

▼display all

Books

  • 絵と図でわかる科学事典 : 星の誕生、ロボットの歴史からいびきのメカニズムまで

    Juzeau, Camille, Rébulard, Morgane, Caradec, Colin, 吉井, 大志, 原, 正人

    グラフィック社  2025.4  ( ISBN:9784766139112

     More details

    Total pages:1冊(ページ付なし)   Language:Japanese

    CiNii Books

    researchmap

  • 生命の時間図鑑 : グラフで見る動植物の体内時計

    Pilcher, Helen, 吉井, 大志

    グラフィック社  2024.1  ( ISBN:9784766138016

     More details

    Total pages:207p   Language:Japanese

    CiNii Books

    researchmap

  • Insect Chronobiology

    Yoshii T, Fukuda A( Role: Contributor ,  Neurocircuitry of Circadian Clocks)

    Springer  2023  ( ISBN:9789819907250

     More details

  • Insect Chronobiology

    Tomioka K, Yoshii T( Role: Contributor ,  Neural and Molecular Mechanisms of Entrainment)

    Springer  2023  ( ISBN:9789819907250

     More details

  • 概日リズムを生み出すショウジョウバエの神経基盤

    関口学, 吉井大志( Role: Joint author)

    アグリバイオ・北隆館  2021.5 

     More details

    Language:Japanese Book type:Scholarly book

  • ショウジョウバエ概日時計の神経ネットワーク

    関口学, 吉井大志( Role: Joint author)

    細胞・ニューサイエンス社  2020.9 

     More details

  • ショウジョウバエの概日リズムは生存に重要か?

    吉井大志( Role: Sole author)

    昆虫と自然・ニューサイエンス社  2020.9 

     More details

  • キイロショウジョウバエの概日温度適応

    梅崎勇次郎, 吉井大志( Role: Joint author)

    比較生理生化学  2017 

     More details

  • The Cricket as a Model Organism: Development, Regeneration and Behavior

    Tomioka K, Uryu O, Kamae Y, Moriyama Y, ASM Saifullah, Yoshii T( Role: Contributor ,  Chapter 6. Molecular approach to the circadian clock mechanism in the cricket.)

    Springer  2017 

     More details

  • ショウジョウバエ中枢概日時計の神経機構

    吉井大志, 富岡憲治( Role: Joint author)

    生体の科学  2016 

     More details

  • 環境Eco選書 昆虫の時計―分子から野外まで―

    吉井大志( Role: Contributor ,  天体航法)

    北隆館  2014 

     More details

  • 昆虫と自然

    吉井大志( Role: Contributor ,  オオカバマダラの“渡り”―太陽コンパスナビゲーションと体内時計―)

    ニューサイエンス社  2013 

     More details

  • キイロショウジョウバエ概日時計の温度サイクル同調機構

    吉井大志, 富岡憲治( Role: Joint author)

    時間生物学  2007 

     More details

  • Circadian Clock as Multi-Oscillator System

    Tomioka K, Yoshii T, ASM. Saifullah( Role: Contributor ,  Multioscillator systems controlling the circadian locomotor rhythm in insects.)

    2003 

     More details

▼display all

MISC

  • Comparative analysis of PDF- and CRY-positive neurons in different Drosophila species

    Rachele Saccon, Christiane Hermann, Taishi Yoshii, Charlotte Helfrich-Foerster

    JOURNAL OF NEUROGENETICS   24   51 - 52   2010.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • The neuropeptides PDF, NPF and ITP operate synergistically in the endogenous clock of Drosophila melanogaster

    Christiane Hermann, Heinrich Dircksen, Charlotte Helfrich-Foerster, Taishi Yoshii

    JOURNAL OF NEUROGENETICS   24   27 - 27   2010.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Differential neuronal expression of three Drosophila ion transport peptide splice forms indicate multiple functions of peptidergic neurons

    Heinrich Dircksen, Aditya Mandali, Taishi Yoshii, Johannes Strauss, Charlotte Helfrich-Foerster, Dick R. Naessel

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY   153A ( 2 )   S79 - S79   2009.6

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    DOI: 10.1016/j.cbpa.2009.04.053

    Web of Science

    researchmap

  • Preferential temperature synchronization of the CRY-negative pacemaker neurons in the Drosophila circadian clock

    Taishi Yoshii, Charlotte Helfrich-Foerster

    JOURNAL OF NEUROGENETICS   23   S82 - S82   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Cryptochrome mediates wavelength-dependent magnetosensitivity in Drosophila melanogaster

    Taishi Yoshii, Margaret Ahmad, Charlotte Helfrich-Foerster

    JOURNAL OF NEUROGENETICS   23   S64 - S64   2009

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • G205 ニカメイガのイネとマコモ系統間における交尾行動と概日リズムの比較

    幡司 梢, 宮竹 貴久, 吉本 明充, 保積 直史, 泉洋 平, 積木 久明, 吉井 大志, 富岡 憲治

    日本応用動物昆虫学会大会講演要旨   ( 51 )   112 - 112   2007.3

     More details

    Language:Japanese   Publisher:日本応用動物昆虫学会  

    CiNii Article

    CiNii Books

    researchmap

  • Analysis of temperature-dependent circadian oscillatory mechanisms by temperature steps in Drosophila melanogaster

    Taishi Yoshii, Kenji Tomioka

    ZOOLOGICAL SCIENCE   23 ( 12 )   1193 - 1193   2006.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Temperature steps induce anticipatory locomotor activity of the circadian clock under constant light in Drosophila melanogaster

    Taishi Yoshii, Kenji Tomioka

    JOURNAL OF NEUROGENETICS   20 ( 3-4 )   263 - 264   2006.7

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • cDNA cloning of clock genes in two species of crickets Gryllus bimaculatus and Modicogryllus siamensis

    Tomoaki Sakamoto, Abdelsalam Salaheldin, Taishi Yoshii, Akira Matsumoto, Kenji Tomioka

    ZOOLOGICAL SCIENCE   22 ( 12 )   1490 - 1490   2005.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Regulation of circadian locomotor rhythms by light and temperature in the fruit fly Drosophila melanogaster

    Kenji Tomioka, Yoko Miyasako, Taishi Yoshii

    ZOOLOGICAL SCIENCE   22 ( 12 )   1423 - 1423   2005.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Search for clock neurons that entrain to light or temperature cycles in Drosophila melanogaster

    Yoko Miyasako, Taishi Yoshii, Kenji Tomioka

    ZOOLOGICAL SCIENCE   22 ( 12 )   1491 - 1491   2005.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Daily cycling of mRNA of pigment dispersing factor gene in the optic lobe of the cricket, Gryllus bimaculatus.

    Abdelsalam Salaheldin, Taishi Yoshii, Kenji Tomioka

    ZOOLOGICAL SCIENCE   22 ( 12 )   1490 - 1490   2005.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Circadian oscillations triggered by temperature steps under constant light in Drosophila melanogaster

    Taishi Yoshii, Kana Fujii, Kenji Tomioka

    ZOOLOGICAL SCIENCE   21 ( 12 )   1324 - 1324   2004.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Possible involvement of temperature-entrainable timing system in arrhythmic mutant flies in Drosophila melanogaster.

    Yoshii T, Tomioka K

    Journal of Photoscience   2002

     More details

▼display all

Presentations

  • Circadian activity rhythms and fitness in Drosophila melanogaster Invited

    Taishi Yoshii, Shoichiro Tamura, Sae Aikawa

    27th International Congress of Entomology (ICE2024 Kyoto)  2024.8.27 

     More details

    Event date: 2024.8.25 - 2024.8.30

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • Trissin/TrissinR signaling pathway mediates activity promoting signal in Drosophila melanogaster

    2022.9.12 

     More details

    Event date: 2022.9.12 - 2022.9.14

    Language:English   Presentation type:Poster presentation  

    Venue:Nagoya, Japan   Country:Japan  

  • ショウジョウバエ時計細胞シグナル伝達経路Trissin/TrissinRの解析

    関口学,吉井大志

    第93回日本動物学会  2022.9.8  日本動物学会

     More details

    Event date: 2022.9.8 - 2022.9.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都新宿区西早稲田 早稲田大学早稲田キャンパス   Country:Japan  

  • Split-GAL4システムによるキイロショウジョウバエ時計細胞群の機能解析

    横佐古達哉,関口学,吉井大志

    第93回日本動物学会  2022.9.8  日本動物学会

     More details

    Event date: 2022.9.8 - 2022.9.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都新宿区西早稲田 早稲田大学早稲田キャンパス   Country:Japan  

  • キイロショウジョウバエにおけるPDF時計細胞群の歩行活動リズムへの影響

    齋藤愛加,横佐古達哉,吉井大志

    第93回日本動物学会  2022.9.8  日本動物学会

     More details

    Event date: 2022.9.8 - 2022.9.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都新宿区西早稲田 早稲田大学早稲田キャンパス   Country:Japan  

  • Roles of morning DN1ps and evening cells in bimodal activity rhythms in Drosophila melanogaster International conference

    M. Sekiguchi, T. Yoshii

    Sapporo Symposium on Biological Rhythm  2022.8.14  Sapporo Symposium on Biological Rhythm

     More details

    Event date: 2022.8.12 - 2022.8.14

    Language:English   Presentation type:Poster presentation  

    Venue:Sapporo, Japan   Country:Japan  

  • Regulation of morning activity through the neuropeptide CNMamide expressed in a subset of clock neurons in the Drosophila melanogaster International conference

    A. Fukuda, T. Yoshii

    Sapporo Symposium on Biological Rhythm  2022.8.14  Sapporo Symposium on Biological Rhythm

     More details

    Event date: 2022.8.12 - 2022.8.14

    Language:English   Presentation type:Poster presentation  

    Venue:Sapporo, Japan   Country:Japan  

  • キイロショウジョウバエ概日時計ネットワーク内における時計細胞の役割

    関口学,吉井大志

    第28回日本時間生物学会学術大会  2021.11.20 

     More details

    Language:Japanese  

  • キイロショウジョウバエ概日時計におけるPDF/CCHa1神経ペプチドの相互作用

    桒野理子,桂万喜,吉井大志

    第92回日本動物学会  2021.9.2 

     More details

    Language:Japanese  

  • キイロショウジョウバエ概日時計ネットワークの解析

    関口学,井上浩太郎,吉井大志

    第92回日本動物学会  2021.9.2 

     More details

    Language:Japanese  

  • キイロショウジョウバエにおけるPDF時計細胞の夕方活動への影響

    齋藤愛加,中西日向子,吉井大志

    第92回日本動物学会  2021.9.2 

     More details

    Language:Japanese  

  • キイロショウジョウバエの概日リズムと高温ストレス耐性

    片岡知樹,吉井大志

    第92回日本動物学会  2021.9.2 

     More details

    Language:Japanese  

  • The BRWD3 gene is required for normal circadian activity rhythms in Drosophila melanogaster

    2019.12.1 

     More details

    Language:English   Presentation type:Poster presentation  

  • ショウジョウバエ時計細胞たちのコミュニケーション

    吉井 大志

    第26回日本時間生物学会学術大会  2019.10.12 

     More details

    Language:Japanese  

    researchmap

  • ショウジョウバエ概日時計を構成する脳内神経ネットワーク

    吉井 大志

    第90回日本動物学会  2019.9.12 

     More details

    Language:Japanese  

    researchmap

  • ショウジョウバエ概日時計における時計細胞間カップリング

    吉井 大志

    第95回日本生理学会大会  2018.3 

     More details

    Language:English   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • キイロショウジョウバエ概日時計の温度同調性

    吉井 大志

    第24回日本時間生物学会学術大会  2017.10 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • Light and temperature entrainment of circadian clock in fruit flies International conference

    Taishi Yoshii

    The 22nd International Congress of Zoology  2016.9 

     More details

    Language:English   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • CRY expression in a subset of Drosophila clock neurons International conference

    Taishi Yoshii

    SRBR 2014  2014.6 

     More details

    Language:English   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • The neuronal network of the circadian clock and its synchronization to environmental cycles in Drosophila melanogaster International conference

    Taishi Yoshii

    2012.9 

     More details

    Language:English   Presentation type:Symposium, workshop panel (public)  

    researchmap

  • Let’s draw actogram with ImageJ! Invited International conference

    Taishi Yoshii

    Assembling a Multi-cellular Circadian Pacemaker  2010.8 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

    researchmap

▼display all

Awards

  • 第19回日本時間生物学会学術大会 優秀ポスター賞

    2012   日本時間生物学会  

     More details

  • 日本動物学会藤井賞

    2003   日本動物学会  

     More details

  • 日本動物学会論文賞

    2003   日本動物学会  

     More details

Research Projects

  • リガンド―受容体の接続から明らかにする概日時計回路

    Grant number:24K09534  2024.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    吉井 大志

      More details

    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

    researchmap

  • 脳を持たないヒドラで探る眠りの起源と変遷

    Grant number:21H02527  2021.04 - 2025.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    伊藤 太一, 楠見 淳子, 吉井 大志, 寺本 孝行

      More details

    Grant amount:\17680000 ( Direct expense: \13600000 、 Indirect expense:\4080000 )

    researchmap

  • 概日時計細胞間の接続様式とその役割

    Grant number:19H03265  2019.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    吉井 大志

      More details

    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

    多くの生物は約24時間周期の環境変化を予測するために、概日時計を持っている。動物においては行動、生理、代謝、内分泌など含む、非常に広範囲の活動に24時間の周期性が観察される。その概日時計の中枢は脳に存在する複数の神経細胞群(時計細胞)であることが分かっている。キイロショウジョウバエにおいては、時計細胞の正確な数が同定されており、時計細胞間の神経ネットワークは非常に注目度の高い研究である。
    キイロショウジョウバエ概日時計の時計細胞間ネットワークを明らかにするために、本年度は概日時計出力因子の機能解析を行った。前年度より解析が続いていたPdf, CCHa1の二重変異体だけでなく、その他の因子の2重変異体、3重変異体の作製を行い、活動リズムの計測を行ってきた。さらに、時計突然変異体との組み合わせも行い、研究を一つまとめる時期に来ている。また、前年度から継続していたSplit-GAL4系統の作製もかなり進んでおり、特定の時計細胞を操作する系統が確立できた。これらの系統を用いて、国際共同研究を進めており、後シナプス結合細胞の探索に貢献することができた。特に、LPN時計細胞群とDN時計細胞群の解析の論文をまとめることができた。その他、複数の国際共同研究に貢献することができ、関連する研究として論文にすることができた。前年度は機器の故障とコロナの影響で、生物発光イメージングはあまり進展がなかったが、本年度は新たな系統の作製に着手し、今後につながる新しい知見を得ることができた。

    researchmap

  • Molecular mechanism of compound eye dependent photic entrainment in insect circadian clocks

    Grant number:18H02480  2018.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Tomioka Kenji

      More details

    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

    The circadian clock sets the peak time for various physiological functions by synchronizing with the daily environmental cycle. In the present study, we have elucidated the molecular mechanism of compound-eye dependent light entrainment and found that light information from the compound eye induces c-fosB expression in the optic lobe, which in turn induces Brwd3, Fbxl3, Fbxl 4, Fbxl 5, Fbxl 7, Fbxl 13, and Fbxl 16 downstream of c-fosB. Their RNAi markedly suppressed light entrainment of behavioral rhythms, in both advance and delay shifts, and CRY2 protein increased during the dark period but decreased with light exposure. The light-dependent decrease in CRY2 protein was suggested to be induced by light-dependent ubiquitination by Brwd3 and Fbxls. A similar mechanism was suggested to be involved in firebrats.

    researchmap

  • Neuronal communications between circadian clock neurons

    Grant number:15H05600  2015.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)  Grant-in-Aid for Young Scientists (A)

    Yoshii Taishi, Charlotte Helfrich-Förster

      More details

    Grant amount:\23400000 ( Direct expense: \18000000 、 Indirect expense:\5400000 )

    Many animals possess the central circadian clock system in brain, which controls activity rhythms. However, their detailed neuronal circuits are still not well understood even in the most advanced model animal, fruit fly, Drosophila melanogaster. In this study, we identified circadian clock neurons that are responsive to environmental changes and a new neurotransmitter CCHamide1 involved in the circadian network. Furthermore, we could contribute to understand the morphological and physiological connections among circadian clock neurons.

    researchmap

  • Functional analysis of putative output factors of the insect circadian clock.

    Grant number:25840121  2013.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    YOSHII Taishi

      More details

    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    In Drosophila melanogaster, the molecular mechanism of circadian clock has been well investigated. However, its neuronal mechanism still remains elusive. The Drosophila brain contains about 150 neurons that are responsible for circadian rhythms in behavior. The motivation of this study is to understand how the circadian clock neurons read out time information, which they generate, to downstream neurons. Here, we found that ITP and CCHa1 are new candidate peptides that function as output factors of the clock neurons.

    researchmap

  • Development of a method for evaluation of ageing in cerebral neuronal circuit using circadian rhythm as a gauge

    Grant number:23657056  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    TOMIOKA Kenji, YOSHII Taishi

      More details

    Grant amount:\3770000 ( Direct expense: \2900000 、 Indirect expense:\870000 )

    The goal of this study was to establish a method to evaluate ageing of cerebral neuronal circuits using overt circadian rhythms as a gauge in Drosophila. Behavioral and molecular analyses revealed that age dependent retardation of a neuropeptide, pigment-dispersing factor (PDF), expression in the dorsal terminal of PDF immunoreactive cerebral clock neurons correlated with the ageing of behavioral rhythms. Overexpression of the peptide strengthened not only the activity rhythm but also molecular oscillation of clock genes even in old flies. Thus measuring the behavioral rhythms may be sufficient to evaluate ageing of the underlying neuronal circuit.

    researchmap

  • Analysis of the circadian brain network resposible for light entrainment in Drosophila melanogaster

    Grant number:23870021  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up  Grant-in-Aid for Research Activity start-up

    YOSHII Taishi, TOMIOKA Kenji

      More details

    Grant amount:\3250000 ( Direct expense: \2500000 、 Indirect expense:\750000 )

    The fruit fly Drosophila melanogasterhas multiple light-input pathways for light entrainment for the circadian clock. Especially, a blue light photo-pigment Cryptochrome (CRY) plays an important role in the pathway. Here, we investigated the brain neural network responsible for the CRY-dependent light entrainment. We found that a subset of pacemaker neurons promotes recovery from 8 h Jetlag in Drosophila, suggesting that there is a neural mechanism involved in the speed of light-entrainment.

    researchmap

  • キイロショウジョウバエにおけるper非依存性振動機構に関する研究

    Grant number:03J08718  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows  Grant-in-Aid for JSPS Fellows

    吉井 大志

      More details

    Grant amount:\2700000 ( Direct expense: \2700000 )

    平成17年度はper非依存性振動機構を解明するために、以下の3点について解析を行った。
    1.前年度の研究結果より、per非依存性振動機構に時計遺伝子dClk、cycが関与することが明らかにされている。本年度の研究では時計遺伝子perとtimが欠損した突然変異系統per^<01>;tim^<01>を用いて、dClk、cyc、vriの周期的発現について恒暗・温度サイクル下で解析を行った。その結果dClkは高温期の前半にピークを示す周期的な発現パターンを示した。一方、cycは低温期にやや発現量が多くなることが分かったが、dClkほど明らかな変動を示さなかった。このことより、per非依存性振動の背後にはdClkの周期的な発現が関与することが示唆された。またdClkの転写抑制に関与するvriは低温期に増加し、dClkとは逆位相で周期的に変動することが明らかとなった。
    2.per^<01>;tim^<01>は温度サイクル下で光依存的に歩行活動の位相が逆転する。その活動相の逆転の背後にはdClkやvriの発現パターンの逆転が考えられる。そこでper^<01>;tim^<01>に恒暗もしくは恒明下で温度サイクルを与え、それらの遺伝子発現について解析した。その結果、dClkとvriの周期的発現は、恒明と恒暗では同じ変動パターンを示した。よって、光依存的に起こる活動相の逆転には時計自身の振動の逆転ではなく、より下流の機構が関与することが示唆された。
    3.光依存的な活動相の逆転に関わる光受容体の同定のために、cry^b、glass^<60j>、so^1とper^<01>との二重突然変異系統を作成して、恒明と恒暗の温度サイクル下で歩行活動を計測した。それぞれの二重突然変異系統ともにper^<01>に比べると不明瞭ではあるが、活動相の逆転が観察された。よってCRYや単眼、複眼といった光受容器が欠損しても光に応答して活動相の逆転が起こることが示唆された。

    researchmap

▼display all

 

Class subject in charge

  • Animal Behavior (2024academic year) 1st and 2nd semester  - 月1~2

  • Ethology I (2024academic year) 1st semester  - 月1~2

  • Ethology II (2024academic year) Second semester  - 月1~2

  • Leadership and SDGs(Earth,Environmental and Life Sciences) (2024academic year) Prophase  - 木1~2

  • Basic Biology 1b (2024academic year) Second semester  - 火1~2

  • Basic Biology 1b (2024academic year) Second semester  - 火1~2

  • Seminar in Insect Chronobiology (2024academic year) Other  - その他

  • Seminar in Insect Chronobiology (2024academic year) Year-round  - その他

  • Chronoecology (2024academic year) Late  - その他

  • Seminar in Environmental Biology and Chronobiology (2024academic year) Year-round  - その他

  • Seminar in Environmental Biology and Chronobiology (2024academic year) Other  - その他

  • Seminar in Environmental Biology and Chronobiology (2024academic year) Year-round  - その他

  • 生物学実験C (2024academic year) 1・2学期

  • Laboratory Course D (2024academic year) special  - その他

  • Biology in English (2024academic year) 3rd and 4th semester  - 金5~6

  • Genetics and Neurobiology (2024academic year) Late  - 月3~4

  • 自然科学入門1(生物学) (2024academic year) 1・2学期

  • Practicum in Museum Studies for Natural Science (2024academic year) special  - その他

  • (L22)Neurogenetics (2024academic year) special  - その他

  • Animal Behavior (2023academic year) 1st and 2nd semester  - 月1~2

  • Ethology I (2023academic year) 1st semester  - 月1~2

  • Ethology II (2023academic year) Second semester  - 月1~2

  • Leadership and SDGs(Earth,Environmental and Life Sciences) (2023academic year) Prophase  - 木1~2

  • Introduction to Earth,Environmental and Life Sciences (2023academic year) Prophase  - 金1~2

  • Basic Biology 1b (2023academic year) Second semester  - 火1~2

  • Seminar in Insect Chronobiology (2023academic year) Other  - その他

  • Chronoecology (2023academic year) Late  - その他

  • Advanced Study (2023academic year) Other  - その他

  • Seminar in Environmental Biology and Chronobiology (2023academic year) Other  - その他

  • Seminar in Environmental Biology and Chronobiology (2023academic year) Other  - その他

  • Seminar in Environmental Biology and Chronobiology (2023academic year) Year-round  - その他

  • Biology Seminar A (2023academic year) special  - その他

  • Biology Seminar B (2023academic year) special  - その他

  • Introduction to Biology II (2023academic year) Second semester  - 水3~4

  • 生物学実験C (2023academic year) 1・2学期

  • Laboratory Course D (2023academic year) special  - その他

  • Seminar in Biology (2023academic year) Year-round  - その他

  • Seminar in Biological Sciences Supervisor (2023academic year) Late  - その他

  • Seminar in Biological Sciences Supervisor (2023academic year) Year-round  - その他

  • Seminar in Biological Sciences Supervisor (2023academic year) Other  - その他

  • Advanced Study in Biology (2023academic year) Year-round  - その他

  • Biology in English (2023academic year) 3rd and 4th semester  - 金5~6

  • Genetics and Neurobiology (2023academic year) Late  - 月3~4

  • Genetics and Neurobiology (2023academic year) Late  - 月3~4

  • Topics of Natural Sciences (2023academic year) Second semester  - 水7~8

  • Practicum in Museum Studies for Natural Science (2023academic year) special  - その他

  • Thesis Research (2023academic year) special  - その他

  • (L22)Neurogenetics (2023academic year) special  - その他

  • Animal Behavior (2022academic year) 1st and 2nd semester  - 月1~2

  • Ethology I (2022academic year) 1st semester  - 月1~2

  • Ethology II (2022academic year) Second semester  - 月1~2

  • Basic Biology 1b (2022academic year) Second semester  - 火1~2

  • Basic Biology 1b (2022academic year) Second semester  - 火1~2

  • Chronoecology (2022academic year) Late  - その他

  • Seminar in Environmental Biology and Chronobiology (2022academic year) Year-round  - その他

  • Biology in English (2022academic year) 3rd and 4th semester  - 金5~6

  • Genetics and Neurobiology (2022academic year) Late  - 月3~4

  • Practicum in Museum Studies for Natural Science (2022academic year) special  - その他

  • Animal Behavior (2021academic year) 1st and 2nd semester  - 月1,月2

  • Ethology I (2021academic year) 1st semester  - 月1,月2

  • Ethology II (2021academic year) Second semester  - 月1,月2

  • Basic Biology 1b (2021academic year) Second semester  - 火1,火2

  • Basic Biology 1b (2021academic year) Second semester  - 火1~2

  • Basic Biology I (2021academic year) 1st and 2nd semester  - 火1,火2

  • 教養生物学実験(分子生物) (2021academic year) 第4学期  - 木5〜8

  • Chronoecology (2021academic year) Late  - その他

  • Seminar in Environmental Biology and Chronobiology (2021academic year) Year-round  - その他

  • 生物学ゼミナール (2021academic year) 特別

  • 生物学ゼミナール A (2021academic year) 特別

  • 生物学ゼミナール B (2021academic year) 特別

  • 生物学実験B (2021academic year) 3・4学期

  • 生物学実験C (2021academic year) 1・2学期

  • 生物学実験D (2021academic year) 3・4学期

  • 生物科学演習 (2021academic year) 通年  - その他

  • 生物科学特別研究 (2021academic year) 通年

  • Biology in English (2021academic year) 3rd and 4th semester  - 金5,金6

  • Biology in English (2021academic year) 3rd and 4th semester  - 金5,金6

  • Genetics and Neurobiology (2021academic year) Late  - 月3,月4

  • Topics of Natural Sciences (2021academic year) Second semester  - 水7~8

  • Practicum in Museum Studies for Natural Science (2021academic year) special  - その他

  • 課題研究 (2021academic year) 特別

  • Animal Behavior (2020academic year) 1st and 2nd semester  - 月1,月2

  • Ethology I (2020academic year) 1st semester  - 月1,月2

  • Ethology II (2020academic year) Second semester  - 月1,月2

  • Basic Biology 1b (2020academic year) Second semester  - 火1,火2

  • Basic Biology I (2020academic year) 1st and 2nd semester  - 火1,火2

  • Chronoecology (2020academic year) Late  - その他

  • Seminar in Environmental Biology and Chronobiology (2020academic year) Year-round  - その他

  • Biology in English (2020academic year) 3rd and 4th semester  - 金5,金6

  • Biology in English (2020academic year) 3rd and 4th semester  - 金5,金6

  • Biology in English II (2020academic year) Third semester  - 金5,金6

  • Genetics and Neurobiology (2020academic year) Late  - 水1,水2

  • Practicum in Museum Studies for Natural Science (2020academic year) special  - その他

▼display all

 

Social Activities

  • NHK番組出演

    Role(s):Commentator, Media coverage

    2022.6

  • NHK番組出演

    Role(s):Commentator, Media coverage

    2021.11

  • 教員免許更新講習

    Role(s):Lecturer

    2021.8

  • NHK番組出演

    Role(s):Panelist

    2021.7