Updated on 2025/12/24

写真a

 
Akihisa Otaka
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Assistant Professor
Position
Assistant Professor
External link

Degree

  • Doctoral Degree ( 2014.3   Kyoto University )

Research Areas

  • Life Science / Biomedical engineering

 

Papers

  • Robust adhesion between solid-state hydroxyapatite and bone tissue through surface demineralization. International journal

    Shichao Xie, Masahiro Okada, Haruyuki Aoyagi, Akihisa Otaka, Xiaofeng Yang, Takayoshi Nakano, Takuya Matsumoto

    Bioactive materials   57   632 - 645   2026.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    OBJECTIVE: Current bone adhesives typically lack adequate mechanical strength, long-term stability, or biocompatibility. To address these limitations, we designed a new adhesion strategy using a solid-state hydroxyapatite (HAp) adhesive in combination with bone surface demineralization. METHODS: Solid-state HAp adhesives were synthesized via wet chemical precipitation and heat treatment. Cortical bone specimens were partially demineralized with phosphoric acid (H3PO4) or ethylenediaminetetraacetic acid (EDTA), and characterized using scanning electron microscopy (SEM) and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). Shear adhesion strength of HAp to demineralized bone was measured over time. In vivo fixation was assessed in rats using micro-computed tomography and histology. Statistical analysis used Tukey-Kramer tests after normality and variance checks. RESULTS: Although the HAp adhesive failed to adhere to non-demineralized bone, effective adhesion was achieved on the surface-demineralized bone tissue. Shear adhesion strength was significantly higher in EDTA-treated samples (238.4 kPa at 10 h) compared to H3PO4-treated samples (102.9 kPa at 1 h), with performance correlating with demineralization depth. ATR-FTIR and SEM analyses revealed that EDTA preserved collagen's triple-helix structure and free water content, both enhancing adhesion. Animal experiments confirmed stable fixation of HAp adhesive to demineralized bone tissue. CONCLUSIONS: Surface demineralization enabled strong adhesion of the solid-state HAp adhesive to bone by exposing collagen swollen with water. Adhesion strength was influenced by structural changes in the demineralized layer, and the adhesive provided stable in vivo fixation, supporting its potential for bone-anchored biomedical applications.

    DOI： 10.1016/j.bioactmat.2025.11.030

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  • Amelioration of Cd-induced bone deterioration by orally administered calcium phosphate

    Ping-chin Sung, Ahmad Bikharudin, Masahiro Okada, Randa Musa, Kenta Uchida, Akihisa Otaka, Tadaaki Matsusaka, Aira Matsugaki, Takayoshi Nakano, Takuya Matsumoto

    APPLIED FOOD RESEARCH   5 ( 2 )   2025.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.afres.2025.101482

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  • High pressure pasteurization: Simultaneous native tissue decellularization and sterilization. Reviewed International journal

    Akihisa Otaka, Takashi Yamamoto, Tetsuji Yamaoka

    Regenerative therapy   26   2 - 8   2024.6

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    INTRODUCTION: Terminal sterilization is important for the clinical applicability of decellularized xenografts. High hydrostatic pressurization (HHP) process is a potential strategy for decellularization and decontamination of xenografts; however, its disinfection efficiency remains poorly elucidated. This study investigated the disinfection efficacy of the HHP process at physiologically relevant 36 °C against difficult-to-kill spore-forming bacteria. METHODS: Bacillus atrophaeus and Geobacillus stearothermophilus were suspended in a pressurization medium with or without antibiotic agents and pressurized under two different HHP procedures: repeated and sustained pressurization. RESULTS: The sustained pressurizing conditions, exploited for the conventional tissue decellularization, did not effectively eliminate the bacteria; however, repeated pressurization greatly increased the disinfection effect. Moreover, the antibiotic-containing pressurization medium further increased the disinfection efficiency to the level required for sterilization. CONCLUSIONS: The optimized high hydrostatic pressurization can be used to sterilize biological tissues during the decellularization process and is a promising strategy for manufacturing tissue-derived healthcare products.

    DOI： 10.1016/j.reth.2024.01.012

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  • Direct Fabrication of Glycoengineered Cells via Photoresponsive Thiol-ene Reaction. Reviewed International journal

    Akihisa Otaka, Taisuke Hirota, Yasuhiko Iwasaki

    ACS biomaterials science & engineering   2024.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    Three-dimensional printing of cell constructs with high-cell density, shape fidelity, and heterogeneous cell populations is an important tool for investigating cell sociology in living tissues but remains challenging. Herein, we propose an artificial intercellular adhesion method using a photoresponsive chemical cue between a thiol-bearing polymer and a methacrylate-bearing cell membrane. This process provided cell fabrication containing 108 cells/mL, embedded multiple cell populations in one structure, and enabled millimeter-sized scaleup. Our approach allows for the artificial cell construction of complex structures and is a promising bioprinting strategy for engineering tissues that are structurally and physiologically relevant.

    DOI： 10.1021/acsbiomaterials.3c01987

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  • Cover Image

    Kenjiro Kiyono, Shun Mabuchi, Akihisa Otaka, Yasuhiko Iwasaki

    Journal of Biomedical Materials Research Part A   2023.5

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    DOI： 10.1002/jbm.a.37546

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  • Bioactive peptide-bearing polylactic acid fibers as a model of the brain tumor-stimulating microenvironment Reviewed

    Wan-Ying Huang, Akihisa Otaka, Satoshi Fujita, Tetsuji Yamaoka

    Polymer Journal   2023.1

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1038/s41428-022-00743-8

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    Other Link： https://www.nature.com/articles/s41428-022-00743-8

  • Bone-targeting polyphosphodiesters that promote osteoblastic differentiation. Reviewed International journal

    Kenjiro Kiyono, Shun Mabuchi, Akihisa Otaka, Yasuhiko Iwasaki

    Journal of biomedical materials research. Part A   111 ( 5 )   714 - 724   2023.1

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    Polymers for pharmaceutical use have been attractive in medical treatments because of the conjugation of multifunctional components and their long circulation time in the blood stream. Bone-targeted drug delivery systems are also no exceptional, and several polymers have been proposed for the treatment of bone diseases, such as cancer metastasis and osteoporosis. Herein, we report that polyphosphodiesters (PPDEs) have a potential to enhance osteoblastic differentiation, and they have a targeting ability to bone tissues in vivo. Two types of PPDEs, poly (ethylene sodium phosphate) (PEP•Na) and poly (propylene sodium phosphate) (PPP•Na), have been synthesized. Regardless of the alkylene structure in the main chain of PPDEs, the gene expression of osteoblast-specific transcription factors and differentiation markers of mouse osteoblastic-like cells (MC3T3-E1 cells) cultured in a differentiation medium was significantly upregulated by the addition of PPDEs. Moreover, it was also clarified that the signaling pathway related to cytoplasmic calcium ions was activated by PPDEs. The mineralization of MC3T3-E1 cells has a similar trend with its gene expression and is synergistically enhanced by PPDEs with β-glycerophosphate. The biodistribution of fluorescence-labeled PPDEs was also determined after intravenous injection in mice. PPDEs accumulated well in the bone through the blood stream, whereas polyphosphotriesters (PPTEs) tended to be excreted from the kidneys. Hydrophilic PEP•Na showed a superior bone affinity as compared with PPP•Na. PPDEs could be candidate polymers for the restoration of bone remodeling and bone-targeting drug delivery platforms.

    DOI： 10.1002/jbm.a.37499

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  • Impact of REDV peptide density and its linker structure on the capture, movement, and adhesion of flowing endothelial progenitor cells in microfluidic devices Reviewed International journal

    Atsushi Mahara, Kazuki Kitagawa, Akihisa Otaka, Takahiko Nakaoki, Kazuhiko Ishihara, Tetsuji Yamaoka

    Materials Science and Engineering: C   129   112381 - 112381   2021.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    Ligand-immobilization to stents and vascular grafts is expected to promote endothelialization by capturing flowing endothelial progenitor cells (EPCs). However, the optimized ligand density and linker structure have not been fully elucidated. Here, we report that flowing EPCs were selectively captured by the REDV peptide conjugated with a short linker. The microchannel surface was modified with the REDV peptide via Gly-Gly-Gly (G3), (Gly-Gly-Gly)3 (G9), and diethylene glycol (diEG) linkers, and the moving velocity and captured ratio were evaluated. On the unmodified microchannels, the moving velocity of the cells exhibited a unimodal distribution similar to the liquid flow. The velocity of the endothelial cells and EPCs on the peptide-immobilized surface indicated a bimodal distribution, and approximately 20 to 30% of cells moved slower than the liquid flow, suggesting that the cells were captured and rolled on the surface. When the immobilized ligand density was lower than 1 molecule/nm2, selective cell capture was observed only in REDV with G3 and diEG linkers, but not in G9 linkers. An in silico study revealed that the G9 linker tends to form a bent structure, and the REDV peptide is oriented to the substrate side. These results indicated that REDV captured the flowing EPC in a sequence-specific manner, and that the short linker was more adequate.

    DOI： 10.1016/j.msec.2021.112381

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  • Lanoconazole-loaded emulsion stabilized with cellulose nanocrystals decorated with polyphosphoesters reduced inflammatory edema in a mouse model Reviewed

    Suphatra Hiranphinyophat, Akihisa Otaka, Syuji Fujii, Yasuhiko Iwasaki

    POLYMER JOURNAL   2021.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41428-021-00548-1

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  • Enhancement of osteoblast differentiation using poly(ethylene sodium phosphate) Reviewed

    Akihisa Otaka, Kenjiro Kiyono, Yasuhiko Iwasaki

    Materialia   15   100977 - 100977   2021.3

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.mtla.2020.100977

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  • Adhesion of Flk1-expressing cells under shear flow in phospholipid polymer-coated immunoaffinity channels Reviewed

    Akihisa Otaka, Atsushi MAHARA, Kazuhiko Ishihara, Tetsuji YAMAOKA

    Journal of Micromechanics and Microengineering   31 ( 4 )   2021.2

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    DOI： 10.1088/1361-6439/abe52a

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    Other Link： https://iopscience.iop.org/article/10.1088/1361-6439/abe52a/pdf

  • Particle-stabilized oil-in-water emulsions as a platform for topical lipophilic drug delivery Reviewed International journal

    Suphatra Hiranphinyophat, Akihisa Otaka, Yuta Asaumi, Syuji Fujii, Yasuhiko Iwasaki

    Colloids and Surfaces B: Biointerfaces   197   111423 - 111423   2021.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    Low-environmental-impact emulsion systems for transdermal drug delivery in topical treatment have gained increasing interest. However, low stability and adverse systemic side effects severely decrease their efficiency. This study proposed a stable oil-in-water (O/W) emulsion loaded with bifonazole (BFZ) as a lipophilic drug stabilized by poly(2-isopropoxy-2-oxo-1,3,2-dioxaphospholane)-modified cellulose nanocrystals (CNC-g-PIPP) as vehicles for topical delivery of lipophilic drugs. We fully characterized stability, BFZ-loaded particle-stabilized emulsions (PEs) for morphology, droplet size, and its distribution. In addition, we evaluated the in vitro drug-releasing capacity and in vitro skin permeation of BFZ in a porcine skin animal model using a side-bi-side® diffusion cell. An O/W BFZ-loaded emulsion stabilized with CNC-g-PIPP particles (BFZ-loaded CP-PE) with a small mean droplet size of 2.54 ± 1.39 μm was developed and was stable for > = 15 days without a significant change in droplet size. The BFZ-loading efficiency in PEs was 83.1 %. BFZ was slowly released over an extended period, and the releasing ratio from BFZ-loaded CP-PE was only 17 % after 48 h. The BFZ-loaded CP-PE showed a ∼4.4-fold increase in BFZ permeation and penetration compared to a conventional surfactant-stabilized emulsion and BFZ control solution. Fluorescence-labeling studies showed that BFZ-loaded CP-PE could well penetrate skin layers from the stratum corneum (SC) to the dermis. In addition, histopathology studies of porcine skin treated with the PE formulation showed an intact SC with unaltered adjacent structures and no observed signs of inflammation. Therefore, the proposed CP-PE shows great potential as a transdermal drug carrier for enhancing lipophilic drug permeation.

    DOI： 10.1016/j.colsurfb.2020.111423

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  • Bone‐targeting phospholipid polymers to solubilize the lipophilic anticancer drug Reviewed International journal

    Akihisa Otaka, Tomoki Yamaguchi, Ryoya Saisho, Toru Hiraga, Yasuhiko Iwasaki

    Journal of Biomedical Materials Research Part A   108 ( 10 )   2090 - 2099   2020.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Current chemotherapy methods have limited effectiveness in eliminating bone metastasis, which leads to a poor prognosis associated with severe bone disorders. To provide regional chemotherapy for this metastatic tumor, a bone-targeting drug carrier was produced by introducing the osteotropic bisphosphonate alendronate (ALN) units into an amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate). The polymer can form nanoparticles with a diameter of less than 30 nm; ALN units were exposed to the outer layer of the particle. A simple mixing procedure was used to encapsulate a hydrophobic anticancer drug, known as docetaxel (DTX), in the polymer nanoparticle, providing a uniform solution of a polymer-DTX complex in the aqueous phase. The complex showed anticancer activities against several breast cancer cell lines, and the complex formation did not hamper the pharmacological effect of DTX. The fluorescence observations evaluated by an in vivo imaging system and fluorescence microscopy showed that the addition of ALN to the polymer-DTX complex enhanced bone accumulation. Bone-targeting phospholipid polymers are potential solubilizing excipients used to formulate DTX and deliver the hydrophobic drug to bone tissues by blood administration.

    DOI： 10.1002/jbm.a.36968

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jbm.a.36968

  • Endocytosis of poly(ethylene sodium phosphate) by macrophages and the effect of polymer length on cellular uptake Invited Reviewed

    Akihisa Otaka, Yasuhiko Iwasaki

    Journal of Industrial and Engineering Chemistry   75   115 - 122   2019.7

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jiec.2019.03.010

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  • Bone-targeting poly(ethylene sodium phosphate) Reviewed International journal

    Yasuhiko Iwasaki, Atsushi Yokota, Akihisa Otaka, Naoyuki Inoue, Akane Yamaguchi, Toru Yoshitomi, Keitaro Yoshimoto, Masashi Neo

    Biomaterials Science   6 ( 1 )   91 - 95   2018

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry ({RSC})  

    Poly(ethylene sodium phosphate) (PEP·Na) showed excellent cytocompatibility and in vivo bone affinity. Moreover, PEP·Na did not interact with thrombin, which is a coagulation-related protein. Because immobilization of therapeutic agents and imaging probes on PEP·Na is easily performed, PEP·Na is a promising polymer for bone-targeted therapies.

    DOI： 10.1039/C7BM00930E

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  • Individual evaluation of cardiac marker expression and self-beating during cardiac differentiation of P19CL6 cells on different culture substrates Reviewed International journal

    Tetsuji Yamaoka, Mitsuhi Hirata, Takaaki Dan, Atsushi Yamashita, Akihisa Otaka, Takahiko Nakaoki, Azizi Miskon, Sachiro Kakinoki, Atsushi Mahara

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   105 ( 4 )   1166 - 1174   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jbm.a.35977

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  • Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel Reviewed International journal

    Akihisa Otaka, Kazuki Kitagawa, Takahiko Nakaoki, Mitsuhi Hirata, Kyoko Fukazawa, Kazuhiko Ishihara, Atsushi Mahara, Tetsuji Yamaoka

    LANGMUIR   33 ( 6 )   1576 - 1582   2017.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.langmuir.6b04070

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  • Early tissue formation on whole-area osteochondral defect of rabbit patella by covering with fibroin sponge Reviewed International journal

    Eiichi Hirakata, Naohide Tomita, Yasushi Tamada, Toru Suguro, Masaaki Nakajima, Yusuke Kambe, Keisuke Yamada, Koji Yamamoto, Masahiro Kawakami, Akihisa Otaka, Hideo Okumura, Shigehiko Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS   104 ( 7 )   1474 - 1482   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jbm.b.33656

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  • Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model Reviewed International journal

    Nobuhiro Kobayashi, Yoshiya Hashimoto, Akihisa Otaka, Tetsuji Yamaoka, Shosuke Morita

    MATERIALS   9 ( 10 )   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ma9100853

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  • Quantification of Cell Co-Migration Occurrences During Cell Aggregation on Fibroin Substrates Reviewed International journal

    Akihisa Otaka, Kazuya Takahashi, Yuji S. Takeda, Yusuke Kambe, Yoshihiko Kuwana, Yasushi Tamada, Naohide Tomita

    TISSUE ENGINEERING PART C-METHODS   20 ( 8 )   671 - 680   2014.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1089/ten.tec.2013.0344

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  • Lateral diffusion in a discrete fluid membrane with immobile particles Reviewed International journal

    Ziya Kalay, Takahiro K. Fujiwara, Akihisa Otaka, Akihiro Kusumi

    Physical Review E - Statistical, Nonlinear, and Soft Matter Physics   89 ( 2 )   022724 - 022724   2014.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Physical Society  

    DOI： 10.1103/PhysRevE.89.022724

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  • Observation and Quantification of Chondrocyte Aggregation Behavior on Fibroin Surfaces Using Voronoi Partition Reviewed International journal

    Akihisa Otaka, Naoyoshi D. Kachi, Naoya Hatano, Yoshihiko Kuwana, Yasushi Tamada, Naohide Tomita

    TISSUE ENGINEERING PART C-METHODS   19 ( 5 )   396 - 404   2013.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1089/ten.tec.2012.0424

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  • Quantitative Evaluation of Fibroblast Migration on a Silk Fibroin Surface and TGFBI Gene Expression Reviewed International journal

    Tomoko Hashimoto, Katsura Kojima, Akihisa Otaka, Yuji S. Takeda, Naohide Tomita, Yasushi Tamada

    JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION   24 ( 2 )   158 - 169   2013.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1163/156856212X629025

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  • How do chondrocytes aggregate on fibroin substrate Reviewed International journal

    Akihisa Otaka, Kazuya Takahashi, Kenji Isshiki, Yusuke Kambe, Katsura Kojima, Yasushi Tamada, Naohide Tomita

    2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)   2013   405 - 408   2013

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/EMBC.2013.6609522

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  • Observation of chondrocyte aggregate formation and internal structure on micropatterned fibroin-coated surface Reviewed International journal

    Naoyoshi D. Kachi, Akihisa Otaka, Seungwoo Sim, Yoshihiko Kuwana, Yasushi Tamada, Junko Sunaga, Taiji Adachi, Naohide Tomita

    BIO-MEDICAL MATERIALS AND ENGINEERING   20 ( 1 )   55 - 63   2010

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3233/BME-2010-0615

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MISC

  • ポリリン酸エステルが卵巣摘出マウスの大腿骨骨密度に与える影響 経時的CTによる評価

    木野 圭一朗, 横田 淳司, 岩崎 泰彦, 大高 晋之, 水谷 正洋, 根尾 昌志

    日本整形外科学会雑誌   94 ( 8 )   S1672 - S1672   2020.9

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    Language：Japanese   Publisher：(公社)日本整形外科学会  

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  • ポリリン酸エステルが卵巣摘出マウスの腰椎骨密度に与える影響 マイクロCTを用いた経時的評価

    水谷 正洋, 横田 淳司, 岩崎 泰彦, 大高 晋之, 木野 圭一朗, 根尾 昌志

    日本整形外科学会雑誌   94 ( 8 )   S1673 - S1673   2020.9

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  • 骨粗鬆症治療を目指したポリマー医薬の開発 Invited

    大高 晋之, 岩﨑 泰彦

    2020.7

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  • 組織再生誘導型小口径脱細胞血管の開発 1~内膜の再生誘導を狙ったリガンドペプチドの役割

    馬原淳, MUNISSO Maria, 大高晋之, 山岡哲二

    再生医療   16   2017

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  • 1H23 Isolation of undifferentiated iPS cells using microfluidic channel immobilized with anti-SSEA-1 antibody

    OTAKA Akihisa, KITAGAWA Kazuki, HIRATA Mitsuhi, FUKAZAWA Kyoko, NAKAOKI Takahiko, ISHIHARA Kazuhiko, MAHARA Atsushi, YAMAOKA Tetsuji

    2016 ( 28 )   "1H23 - 1"-"1H23-4"   2016.1

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    Language：Japanese   Publisher：The Japan Society of Mechanical Engineers  

    We have been proposing a label-free cell separation device called "cell rolling column" in which cells selectively roll on antibody-immobilized column surfaces. The aim of this study was to investigate the possibilities of separation of undifferentiated induced pluripotent stem (iPS) cells from heterogeneous cells. Three types of phospholipid polymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-w-butyl methacrylate (BMA)-co-p-nitrophenyl oxycarbonyl poly (ethylene glycol) methacrylate (MEONP)], were synthesized with monomer feed ratios of MPC:BMA:MEOPN of 30:60:10 (PMBN 10), 33:66:1 (PMBN 1) and 33:67:0 (PMBN 0). A microfluidic channel was coated with PMBNs and immobilized with antibodies to stage-specific embryonic antigen 1 (SSEA-1), known as a pluripotency marker for murine cells. Suspended iPS cells were infused into the channels, and cell rolling speed and SSEA-1 expression were investigated. The results indicate that PMBN 10 coated channel showed highest density of anti-SSEA-1 antibody immobilization, and SSEA-1 positive cells were rolled on the surface. It is concluded that the cell rolling column can be a promising tool for a cell separation system for undifferentiated iPS cells.

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  • 抗体固定化マイクロ流路を用いたSSEA-1発現量による未分化iPS細胞のラベルフリー分離

    大高晋之, 平田みつひ, 馬原淳, 山岡哲二

    高分子学会医用高分子シンポジウム講演要旨集   45th   2016

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  • ペプチド修飾脱細胞化人工血管の開発 1~脱細胞化小口径人工血管による血管組織再生と中期開存性

    馬原淳, 北井麻里奈, 北井麻里奈, 大高晋之, ムニッソ マリア, 大矢裕一, 山岡哲二

    再生医療   15   2016

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  • 抗Flk1抗体固定化細胞ローリングカラム内のiPS細胞由来心筋前駆細胞の移動速度評価

    北川和宜, 北川和宜, 大高晋之, 平田みつひ, 深澤今日子, 中沖隆彦, 石原一彦, 馬原淳, 山岡哲二

    高分子学会予稿集(CD-ROM)   65 ( 1 )   2016

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  • 細胞ローリングカラムを用いた未分化iPS細胞の分離

    大高晋之, 北川和宜, 北川和宜, 平田みつひ, 深澤今日子, 中沖隆彦, 石原一彦, 馬原淳, 山岡哲二

    再生医療   15   2016

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  • 抗SSEA-1抗体を固定化したPMBNコート流路による未分化iPS細胞の分離

    大高晋之, 北川和宜, 北川和宜, 平田みつひ, 深澤今日子, 中沖隆彦, 石原一彦, 馬原淳, 山岡哲二

    日本バイオマテリアル学会大会予稿集   37th   2015

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  • ペプチド修飾脱細胞人工血管の3ケ月開存における組織再生

    馬原淳, 北井麻里奈, 北井麻里奈, 大高晋之, ムニッソ マリア, 大矢裕一, 山岡哲二

    人工臓器(日本人工臓器学会)   44 ( 2 )   2015

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  • 小口径脱細胞血管の開存化のためのin vivo細胞補足工学

    山岡哲二, 染川将太, 染川将太, 小林直樹, 小林直樹, 大高晋之, 北井麻里奈, 北井麻里奈, ムニッソ マリア, 馬原淳

    人工臓器(日本人工臓器学会)   44 ( 2 )   2015

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  • 未分化iPS細胞の分離のための抗体固定化ポリマー被覆マイクロ流路の開発

    大高晋之, 北川和宜, 北川和宜, 平田みつひ, 中沖隆彦, 石原一彦, 馬原淳, 山岡哲二

    高分子学会医用高分子シンポジウム講演要旨集   44th   2015

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  • 抗体固定化マイクロ流路を用いたマーカー未修飾幹細胞の分離

    大高晋之, 馬原淳, 山岡哲二

    再生医療   14   2015

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  • ペプチド修飾脱細胞血管の開存と組織再生機構の解明

    馬原淳, 北井麻里奈, 北井麻里奈, 大高晋之, MUNISSO Maria, 大矢裕一, 山岡哲二

    日本バイオマテリアル学会大会予稿集   37th   2015

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  • ペプチド修飾脱細胞人工血管の組織反応と再生機構の解析

    馬原淳, 北井麻里奈, 北井麻里奈, 大高晋之, MUNISSO Maria, 大矢裕一, 山岡哲二

    高分子学会予稿集(CD-ROM)   64 ( 2 )   2015

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  • ペプチド修飾小口径脱細胞血管の開存メカニズム

    馬原淳, 北井麻里奈, 北井麻里奈, 大高晋之, ムニッソマリア, 大矢裕一, 山岡哲二

    高分子学会医用高分子シンポジウム講演要旨集   44th   2015

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  • 2F23 Quantitative analysis of trajectory data during cell aggregate formation

    OTAKA Akihisa, ISSHIKI Kenji, SANO Kumpei, KOJIMA Katsura, TAMADA Yasushi, TOMITA Naohide

    2014 ( 26 )   491 - 492   2014.1

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  • B102 Verification of quantitative method for evaluating density dependent migration of cells

    OTAKA Akihisa, SANO Kumpei, ISSHIKI Kenji, TAKAHASHI Kazuya, KOJIMA Katsura, TAMADA Yasushi, TOMITA Naohide

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering   2013 ( 24 )   21 - 22   2013.10

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  • 2S10 How the cells aggregate

    TOMITA Naohide, OTAKA Akihisa, TAKAHASHI Kazuya, ISSIKI Kenji, KOJIMA Katsura, TAMADA Yasushi

    2013 ( 25 )   231 - 232   2013.1

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  • B204 The Effect of different Bio-Environment towards Chondrocyte's Aggregation Movement

    Takahashi Kazuya, Otaka Akihisa, Kambe Yusuke, Kojima Katsura, Tamada Yasushi, Tomita Naohide

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering   2012 ( 23 )   133 - 134   2012.10

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  • B205 The relation between direction of cell migration and cell density gradient in the formation of cartilage cell aggregation

    Isshiki Kenji, Otaka Akihisa, Takahashi Kazuya, Kojima Katsura, Tamada Yasushi, Tomita Naohide

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering   2012 ( 23 )   135 - 136   2012.10

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  • J028013 The effect of cell density for cell aggregation behavior

    ISSHIKI Kenji, OTAKA Akihisa, TAKAHASHI Kazuya, TAKEDA Yuji, KOJIMA Katsura, TAMADA Yasushi, TOMITA Naohide

    Mechanical Engineering Congress, Japan   2012   "J028013 - 1"-"J028013-5"   2012.9

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    Several reports suggest that cell aggregation is important in the course of tissue regeneration or development. The cell aggregation is thought to be affected by direct contact such as cell-material interaction and/or cell-cell interaction and by indirect effects such as substance diffusion. In this study, we estimated whether cells in distant places have an effect on each other or not, and observed cartilage cell movement on fibroin and fibronectin by using time-lapse microscopic recordings. The cellular behavior was analyzed by comparing the relation between cell density distribution and direction of cell migration to the Moore neighborhood referring to the Bonnet's method. The results suggest that the direction of cartilage cell migration seems to have no relation to cell density distribution regardless of cell aggregation behavior. Judging from our previous report showing that aggregation rate and cell-cell adhesion rate of chondrocyte are much higher on fibroin than on fibronectin, the chondrocytic aggregate formation characteristically observed on the fibroin surface may be attributed to cell-cell adhesion.

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  • J028011 The effect of cell-cell adhesion on the ability of chondrogenesis

    TAKAHASHI Kazuya, OTAKA Akihisa, KAMBE Yusuke, KOJIMA Katsura, TAMADA Yasushi, TOMITA Naohide

    Mechanical Engineering Congress, Japan   2012   "J028011 - 1"-"J028011-5"   2012.9

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    We reported that we could treat the defect of patella by using fibroin which is from silkworm cocoon. And we also reported that we observed the rich cartilage matrixes and chondrocyte's aggregation in fibroin. So we focused on the chondrocyte's aggregation on fibroin and tried to develop the method of analyzing it quantitatively for evaluating the effect of cell-cell interaction on aggregated chondrocyte cultured on fibroin or other matrixes. First, we tried to define cell aggregation by comparing chondrocyte's aggregation on fibroin with random walk models. And second, we cultured chondrocytes on fibroin, fibronectin and collagen type I and on we evaluated the amount of GAG production and the number of chondrocytes cultured on each matrixes. As a result, at first we found that on fibroin the number of cells which are 10-20 |0.m away from other cells is especially high. And also, they keep cell-cell adhesion for more than an hour. And second, it is suggested that the chondrocyte on fibroin is easier to aggregate than that on fibronectin or collagen type I. What is more, the chondrocyte on fibroin has a higher ability to produce GAG than that on other matrixes. From these results it is suggested that the aggregation movement on fibroin is caused by cell-cell adhesion but also such cell-cell interaction is affected by cell-matrix interaction. In addition it is also suggested that the aggregation movement is related to the ability of chondrogenesis.

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  • 7F34 Quantitative evaluation of chondrocyte aggregating process on various scaffold materials

    OTAKA Akihisa, TAMADA Yasushi, KUWANA Yoshihiko, HATANO Naoya, TOMITA Naohide

    2012 ( 24 )   "7F34 - 1"-"7F34-2"   2012.1

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  • フィブロイン上の細胞間接着が軟骨組織形成に及ぼす影響

    高橋和也, 大高晋之, 神戸祐介, 小島桂, 玉田靖, 富田直秀

    再生医療   11   2012

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  • 細胞密度分布が細胞凝集挙動に及ぼす影響の評価方法の提案

    一色健司, 大高晋之, 高橋和也, 武田祐史, 小島桂, 玉田靖, 富田直秀

    再生医療   11   2012

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  • シルク由来材料表面上での繊維芽細胞の運動性の解析

    橋本朋子, 小島桂, 武田祐史, 大高晋之, 富田直秀, 玉田靖

    高分子学会予稿集(CD-ROM)   60 ( 1 Disk1 )   2011

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  • 多孔質径の異なるシルクスポンジ内で培養した軟骨細胞の遺伝子発現

    玉田靖, 小島桂, 川上雅弘, 川上雅弘, 大高晋之, 神戸祐介, 武田祐史, 富田直秀

    高分子学会予稿集(CD-ROM)   59 ( 1 Disk1 )   2010

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  • L-RGDSx2フィブロイン基質上での軟骨細胞凝集体形成挙動

    武田祐史, 大高晋之, 小島桂, 玉田靖, 富田直秀

    再生医療   9   2010

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Presentations

  • Non-invasive spectroscopic–machine learning platform for evaluating quality of mineralized tissues

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    Event date： 2026.3.16 - 2026.3.20

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  • 酸処理チタンと骨との相互作用に関する研究

    青柳陽之,岡田正弘,大高晋之,中野貴由,松本卓也

    令和7（2025）年度近畿・中四国地方会セミナー  2025.12.13  日本歯科理工学会

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    Event date： 2025.12.13

    Venue：兵庫  

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  • FTIRスペクトルを用いた新規・非侵襲硬組織評価

    大高 晋之,許 国琛,周 鵬凱,楊 暁鋒,李 旋,岡田 正弘,松本 卓也

    第27回生体関連セラミックス討論会  2025.12.12  日本セラミックス協会

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    Event date： 2025.12.12

    Venue：香川  

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  • Machine Learning–Driven FTIR Analysis for Detecting Collagen Cross-Linking in Biological Tissues

    2025.12.10 

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    Event date： 2025.12.8 - 2025.12.13

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  • Collagen Exposure by Acid Digestion of Bone Tissue

    2025.12.10 

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    Event date： 2025.12.8 - 2025.12.13

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  • 赤外スペクトルを用いた非侵襲デジタル歯質診断

    大高 晋之,許 国琛,周 鵬凱,楊 暁鋒,李 旋,岡田 正弘,松本 卓也

    第 46 回岡山歯学会・学術大会  2025.11.30  岡山歯学会

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    Event date： 2025.11.30

    Venue：岡山  

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  • 硬組織に対する多孔質ハイドロキシアパタイトの接着固定とメカニズムの検討

    謝世超,岡田正弘,青柳陽之,大高晋之,楊暁峰,中野貴由,松本卓也

    第47回日本バイオマテリアル学会大会  2025.11.10  日本バイオマテリアル学会

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    Event date： 2025.11.9 - 2025.11.11

    Venue：東京  

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  • Prediction of Bone Quality via Non-Invasive FTIR Assessment of Skin Collagen Cross-Linking

    2025.11.10 

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    Event date： 2025.11.9 - 2025.11.11

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  • Quantitative Assessment of Bone Collagen Cross-linking Through the Synergistic Application of Infrared Spectroscopy and Machine Learning

    2025.11.10 

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    Event date： 2025.11.9 - 2025.11.11

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  • 酸処理チタンと骨との相互作用に関する研究

    青柳陽之,岡田正弘,大高晋之,中野貴由,松本卓也

    第47回日本バイオマテリアル学会大会  2025.11.10  日本バイオマテリアル学会

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    Event date： 2025.11.9 - 2025.11.11

    Venue：東京  

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  • 表面脱灰骨組織への固体ハイドロキシアパタイト接着剤

    謝世超,岡田正弘,青柳陽之,大高晋之,楊暁峰,中野貴由,松本卓也

    第47回日本バイオマテリアル学会大会  2025.11.10  日本バイオマテリアル学会

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    Event date： 2025.11.9 - 2025.11.11

    Venue：東京  

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  • Data-driven IR spectral analysis for evaluating collagen crosslink in biological tissues

    2025.11.10 

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    Event date： 2025.11.9 - 2025.11.11

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  • Analysis of Collagen Cross-Linking for Tooth Quality Assessment using Machine Learning-Assisted FTIR

    2025.11.10 

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    Event date： 2025.11.9 - 2025.11.11

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  • Non-invasive digital dental diagnosis using infrared spectroscopy

    2025.11.8 

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    Event date： 2025.11.8

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Research Projects

  • Cation-free mRNA delivery careers mimicking ribonucleoprotein

    Grant number：23K11861  2023.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    大高 晋之, 山岡 哲二

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    Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）

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  • Photoresponsive 3D structure formation using glycoengineered cells

    Grant number：19K20701  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Otaka Akihisa

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    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    (1) We proposed a novel method to initiate intracellular adhesion by a photo-responsive cross-linking reaction using glycoengineered cells cultured with methacryloyl mannosamine. Using this method, we can fabricate a high cell density 3D structures composed of variety of cell types, and it will become possible to construct complicated structures like a living tissues. (2) We synthesized zwitterionic methacryloyl arginine using the same reaction scheme used above and found that monomers can capture nucleic acids. These findings indicate the methacryloyl arginine can be used as an mRNA condensation agent and will be a novel composition unit of mRNA carrier.

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  • Investigation of optimal polyphosphoester structure for bone treatment

    Grant number：19H04474  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Iwasaki Yasuhiko

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    Grant amount：\16380000 （ Direct expense: \12600000 、 Indirect expense：\3780000 ）

    Biodegradable polyphosphoesters (PPEs) are of increasing interest due to their promising biomedical applications. Polyphosphodiesters (PPDEs) have mineral affinity and therefore are promising polymer drug candidates for bone disease treatment. However, their effects on osteoblasts are still unclear. In this study, we investigated the effect of PPDEs on bone cell function and clarified that PPDEs upregulated osteoblastic differentiation whereas downregulated osteoclast differentiation. Furthermore, biodistribution of PPDEs in osteoporosis mouse model was studied. PPDEs selectively accumulated in bone tissue. Due to the molecular diversity of polyphosphoesters, various types of polymeric prodrugs for bone disease treatment can be designed based on PPDEs.

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  • Development of a new DCM therapeutic strategy, DNCS, to remove etiologic factors in the blood

    Grant number：15H04923  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Minatoya Kenji

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    Grant amount：\16380000 （ Direct expense: \12600000 、 Indirect expense：\3780000 ）

    A conjugate molecule of a etiologic molecule-binding molecule and an ApoE molecule that binds to the liver LDL receptor was synthesized as a "navigator" molecule, which is a novel drug that navigate the target etiologic molecule to the liver and reduce it blood level. First, it was confirmed in vitro that the navigator molecule binds well with the target molecule, and hepatocyte selectively uptake the navigating. When cultured at 37 degree C the fluorescence intensity in the hepatocytes became much lower than when cultured at 4 degree C, which suggests that the navigator was taken up by the hepatocytes and degraded in the cells. Furthermore, we investigated the body distribution of the navigator molecule in the mouse, and found that the liver accumulated was significantly higher, indicating the basic POC.

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  • Evaluation of the effect of cell enrichment process on iPS cell differentiation

    Grant number：15K21696  2015.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Otaka Akihisa

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    Grant amount：\3250000 （ Direct expense: \2500000 、 Indirect expense：\750000 ）

    Cell rolling behaviors resulting from dynamic cell adhesion to a solid surface with immobilized antibodies have been investigated for label-free cell separation. Here, we investigated the effect of cell rolling behaviors on cardiomyocyte differentiation of induced pluripotent stem cells. After mesoderm induction, proportion of Flk1 positive cells was increased from 27% to 49%, by fractionating cells using cell rolling column, in which anti-Flk1 antibody was successively immobilized on poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyl oxycarbonyl poly[ethylene glycol] methacrylate) coated surfaces. After separation, the caidiomyocyte-differentiation potential was decreased with the increase of the proportion of Flk1 positive cells. It have been reported the Flk1 positive cells show high potential of caidiomyocyte differentiation. Collectively, our data demonstrate that the cell enrichment process may affect cell differentiation properties.

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Class subject in charge

  • Biomaterials for Hard Tssue Substitution （2025academic year） Second semester  - 火5,火6

  • Training in Biomaterials （2025academic year） 1st semester  - 火7,火8

  • Training in Biomaterials for Hard Tissue Substitution （2025academic year） Second semester  - 火7,火8

  • Biomaterials （2025academic year） Concentration  - その他

  • Biomaterials 1 （2025academic year） 1st semester  - 火5,火6

  • Biomaterials 2 （2025academic year） Third semester  - 火7～8

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