Faculty Profiles - ICHINOSE Yuki

MISC - ICHINOSE Yuki

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植物の病原菌に体する受容性と抵抗性

新版分子レベルからみた植物の耐病性 (細胞工学別冊)，(島本功ら編) 秀潤社 pp.74-81. 2004

PDR-type ABC transporter and tobacco defense response against pathogen

Y Ichinose, D Schenke, M Sasabe, Y Inagaki, K Toyoda, T Shiraishi

PLANT AND CELL PHYSIOLOGY 45 S1 - S1 2004

Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101

D Schenke, M Sasabe, K Toyoda, Y Inagaki, T Shiraishi, Y Ichinose

GENES & GENETIC SYSTEMS 78 ( 6 ) 409 - 418 2003.12

Authorship：Last author,　Corresponding author Language：English Publisher：GENETICS SOC JAPAN

Here we report the genomic structure including the promoter sequence and coding region of NtPDRI (Nicotiana tabacum Pleiotropic Drug Resistance 1), which is an elicitor-responsive gene encoding an ATP binding cassette (ABC) transporter that might be involved in the defense response in tobacco, as we reported recently. The NtPDR1 gene consists of 20 exons and 19 introns. Among the introns, the first and fifth are much larger than the others and harbor typical miniature inverted-repeat transposable elements (MITEs). One of the MITE elements in the first intron, termed NtToya1, belongs to the Toya family that was recently described in rice, while the other element in the fifth intron, termed NtStowaway101, shows high homology with the Stowaway elements of the IS630-Tc1-mariner family. Many of the genes we found to harbor Toya and Stowaway elements in Nicotiana species by BLAST search are also involved in stress responses or plant-pathogen interactions. The existence of putative cis-elements (a GCC box, three W boxes, and several JA-responsive elements) in the promoter region supports our previous finding that this gene is strongly inducible by elicitation and methyljasmonate, and that this ABC transporter might be essential for plant defense responses. Furthermore, Southern blot analysis and PCR amplification of the introns harboring the MITE-like elements from genomic DNA of three Nicotiana species suggests that NtPDR1 originated from N. sylvestris.

DOI： 10.1266/ggs.78.409

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Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

F Taguchi, R Shimizu, R Nakajima, K Toyoda, T Shiraishi, Y Ichinose

PLANT PHYSIOLOGY AND BIOCHEMISTRY 41 ( 2 ) 165 - 174 2003.2

Language：English Publisher：EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

DOI： 10.1016/S0981-9428(02)00018-9

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Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea.

The Scientific Reports of The Faculty of Agriculture, Okayama University. 92, in press 2003

Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

J. Gen. Plant Pathol. 69 (4) 244-249. 2003

Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

J. Gen. Plant Pathol. 69 (4) 244-249. 2003

Role of flagella and flagellin in plant-Pseudomonas syrignae interactions. In Japan/ Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe (N. Sako, H. Yaegashi and M.-K. Yang eds.)

Showado Co. (Saga, Japan) pp. 158-166. 2003

Phylogenetic Classification of Dof type Transcription Factors in Pea (Pisum sativum)

Naoki Nakamura, Mizuri Marutani, Shiroh Sanematsu, Kazuhiro Toyoda, Yoshi-shige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Plant Biotechnology 20 ( 3 ) 247 - 252 2003

Language：English

The Dof protein family is a group of plant transcription factors carrying highly conserved 52 residues referred to as the Dof domain, and belongs to the C4 zinc-finger transcription factors. We isolated various PsDof genes by PCR using the Dof-domain nucleotide sequence of the PsDofl gene from a cDNA library of elicitor-treated pea epicotyls, and these isolated PsDof genes were then classified phylogenetically. Since the obtained genes (PsDofl to PsDofT) were scattered over various positions of the phylogenetic tree, they were expected to perform various functions as Dof-type transcription factors. From their positions in the tree, it is expected that the PsDofZ and PsDof5 genes are defense related, as is the PsDofl gene. © 2003, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.

DOI： 10.5511/plantbiotechnology.20.247

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Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

J. Gen. Plant Pathol. 69 (6) In press 2003

Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

J. Gen. Plant Pathol. 69 (6) In press 2003

Role of flagella and flagellin in plant - Pseudomonas syringae interactions

Y Ichinose, R Shimizu, F Taguchi, K Takeuchi, M Marutani, T Mukaihara, Y Inagaki, K Toyoda, T Shiraishi

PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS pp. 311-318. 311 - 318 2003

Authorship：Lead author,　Corresponding author Language：English Publisher：SPRINGER

The role of flagella and monomer flagellin of Pseudomonas syringae pv. tabaci in plant-bacteria interactions was investigated by using non-polar fliC or fliD mutants. These mutants deleted the open reading frames for fliC and fliD, respectively, and both mutants lost all flagella and motility. The DeltafliC mutant did not produce flagellin, whereas DeltafliD mutant, that lost HAP2 protein, secreted a large amount of monomer flagellin in the culture medium. Inoculation of tomato leaves with wild type and DeltafliD mutant of P. s. pv. tabaci induced HR, whereas the DeltafliC mutant caused symptom-like change and propagated as P. s. pv. tomato. In tomato suspension cultured cells, wild type P. s. pv. tabaci induced visible HR-like changes. The DeltafliC mutant did not induce the HR, but the response was activated by the DeltafliD mutant. The expression of typical defence genes such as PAL and hsr203J was rapidly and strongly induced by inoculation with the DeltafliD mutant compared to inoculation with wild type P. s. pv. tabaci. On the other hand, both fliC and fliD mutants were reduced in virulence when inoculated into host tobacco leaves. Furthermore, complementation of fliC gene in DeltafliC mutant restored motility and HR-inducing ability in tomato, and virulence in tobacco. These results suggest that the monomer flagellin of P. s. pv. tabaci is an essential factor in the elicitation of HR in non host tomato cells, and flagella are required for complete virulence in host tobacco cells.

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Post-translational modification of flagellin determines the specificity of HR induction.

Plant Cell Physiol. 44 (3) 342-349. 2003

Flagella-mediated Interactions between Phytopathogenic Bacteria and Plants

ICHINOSE Yuki, TAGUCHI Fumiko, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori

KAGAKU TO SEIBUTSU 41 (8) 511-516 ( 8 ) 511 - 516 2003

Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea

Seki Hikaru, Marutani Mizuri, Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

92, in press ( 1 ) 21 - 26 2003

Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

J. Gen. Plant Pathol. 69: 33-38. 2003

Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

J. Gen. Plant Pathol. 69: 33-38. 2003

The DfliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells.

Mol. Genet. Genomics 269: 21-30. 2003

Showado Co. (Saga, Japan) pp. 158-166. 2003

(321)Hypersensitive Response-Inducing Factors Produced by Pseudomonas syringae (5) Specific HR-Inducing Activity of Flagellins from Several Pathovars of P. syringae Pseudomonas

Taguchi F, Shimizu R, Takeuchi K, Ikeda Y, Inagaki Y, Shiraishi T, Ichinose Y

Annals of the Phytopathological Society of Japan 68 ( 2 ) 242 - 243 2002.8

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