Faculty Profiles - ICHINOSE Yuki

MISC - ICHINOSE Yuki

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PHENYLALANINE AMMONIA-LYASE GENES FROM PISUM-SATIVUM - STRUCTURE, ORGAN-SPECIFIC EXPRESSION AND REGULATION BY FUNGAL ELICITOR AND SUPPRESSOR

T YAMADA, Y TANAKA, P SRIPRASERTSAK, H KATO, T HASHIMOTO, S KAWAMATA, Y ICHINOSE, H KATO, T SHIRAISHI, H OKU

PLANT AND CELL PHYSIOLOGY 33 ( 6 ) 715 - 725 1992.9

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Genomic DNA clones representing two members of a small multigene family of phenylalanine ammonia-lyase (pal) in pea,designated PSPAL1 and PSPAL2, were obtained and the nucleotide sequences were determined. PSPAL1 and PSPAL2 each contain a single intron but the sizes of the introns are different. Using specific oligonucleotide probes from the 5'-noncoding region of PSPAL1 and PSPAL2, we showed that the accumulation of both PSPAL1 and PSPAL2 transcripts was induced in epicotyl tissues by treatment with the fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and transcripts of both PSPAL1 and PSPAL2 accumulated at high levels in roots and at moderate level in stems but at very low levels in the upper part of the plant that included leaves and flower organs. A chimeric gene carrying 480 bp of the putative promoter fragment of PSPAL1 connected to the bacterial cat gene and the nos terminator was introduced by electroporation into pea protoplasts and the induction by fungal elicitor and suppression by suppressor or orthovanadate, an inhibitor for plasma membrane ATPase, were examined by an transient assay for CAT activity. CAT activity was induced by the treatment with fungal elicitor but suppressed by the fungal suppressor and by orthovanadate.

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AN ENDOGENOUS SUPPRESSOR OF THE DEFENSE RESPONSE IN PISUM-SATIVUM

K NASU, T SHIRAISHI, H YOSHIOKA, N HORI, Y ICHINOSE, T YAMADA, H OKU

PLANT AND CELL PHYSIOLOGY 33 ( 5 ) 617 - 626 1992.7

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Healthy pea plants contain a substance, tentatively called "endogenous suppressor", which specifically suppresses the accumulation of pisatin in pea plants that is induced by treatment with CuCl2 or an elicitor from Mycosphaerella pinodes. This suppressor elicits the accumulation of phytoalexins in other legumes, such as kidney bean, soybean and cowpea. The endogenous suppressor functions to delay the accumulation of pisatin, the activation of phenylalanine ammonialyase (PAL) and the accumulation of mRNAs for PAL and chalcone synthase induced by the elicitor from M. pinodes. The substance specifically induces susceptibility to nonpathogens, such as Mycosphaerella ligulicola and M. melonis, in pea out of four species of legume tested, but the effect is not cultivar-specific. Thus, the endogenous suppressor in healthy pea plants suppresses a series of self-defense reactions and induces susceptibility in pea plants in a species-specific manner, being similar to the exogenous fungal suppressor from the pea pathogen, M. pinodes.

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2-SUPPRESSORS, SUPPRESCINS-A AND SUPPRESCINS-B, SECRETED BY A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

T SHIRAISHI, K SAITOH, HM KIM, T KATO, M TAHARA, H OKU, T YAMADA, Y ICHINOSE

PLANT AND CELL PHYSIOLOGY 33 ( 5 ) 663 - 667 1992.7

Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Two mucin-type glycopeptides that suppressed the production of pisatin, a phytoalexin of pea, were purified from a pea pathogen, Mycosphaerella pinodes. The structures of Supprescin A (Mr, 452) and Supprescin B (Mr, 959) were determined by an analysis of amino acid sequences and C-13- and H-1-NMR.

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REGULATION OF POLYPHOSPHOINOSITIDE METABOLISM IN PEA PLASMA-MEMBRANES BY ELICITOR AND SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

K TOYODA, T SHIRAISHI, H YOSHIOKA, T YAMADA, Y ICHINOSE, H OKU

PLANT AND CELL PHYSIOLOGY 33 ( 4 ) 445 - 452 1992.6

Language：English Publisher：OXFORD UNIV PRESS

Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on polyphosphoinositide metabolism in pea plasma membranes were examined in vitro. Lipid phosphorylation in the isolated pea plasma membrane was drastically stimulated by the elicitor, but markedly inhibited by the suppressor. A similar inhibitory effect was observed by the treatment with orthovanadate or K-252a that blocked pisatin production induced by the elicitor. Neomycin, an aminoglycoside antibiotic that interacts with the polyphosphoinositide metabolism, also affected the lipid phosphorylation in vitro and blocked the elicitor-induced accumulation of pisatin in vivo. These results suggest that rapid changes of polyphosphoinositide metabolism in pea plasma membranes is one of indispensable processes during the elicitation of defense responses.

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ENDOGENOUS ELICITOR PRESENT IN BARLEY-SEEDS .2. MECHANISMS OF THE INDUCTION OF THE RESISTANCE IN BARLEY LEAVES TO ERYSIPHE-GRAMINIS

T HIRAMOTO, R TOBIMATSU, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT 135 ( 2 ) 167 - 176 1992.6

Language：English Publisher：BLACKWELL WISSENSCHAFTS-VERLAG GMBH

Endogenous elicitor(s) present in barley seeds induce the accumulation of antifungal substances effective against powdery mildew fungi. Antifungal substances induced by the treatment with barley seeds extract coincides with the one induced by the inoculation with compatible or incompatible races of powdery mildew fungi on thin-layer chromatography analysis. The resistance induced by the treatment with barley seeds extract correlates to the accumulation of the antifungal substances in barley leaves. Furthermore, the activity of phenylalanine ammonia-lyase was also induced by the treatment with barley seeds extract.

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TRANSIENT EXPRESSION IN ELECTROPORATED PEA PROTOPLASTS - ELICITOR RESPONSIVENESS OF A PHENYLALANINE AMMONIA-LYASE PROMOTER

T HASHIMOTO, T YAMADA, A TADA, S KAWAMATA, Y TANAKA, P SRIPRASERTSAK, Y ICHINOSE, H KATO, S IZUTSU, T SHIRAISHI, H OKU, Y OHTSUKI

PLANT CELL REPORTS 11 ( 4 ) 183 - 187 1992.5

Language：English Publisher：SPRINGER VERLAG

High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (> 100-mu-g/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

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MOLECULAR-CLONING OF CHALCONE SYNTHASE CDNAS FROM PISUM-SATIVUM

Y ICHINOSE, S KAWAMATA, T YAMADA, CC AN, T KAJIWARA, T SHIRAISHI, H OKU

PLANT MOLECULAR BIOLOGY 18 ( 5 ) 1009 - 1012 1992.3

ORTHOVANADATE SUPPRESSES ACCUMULATION OF PHENYLALANINE AMMONIA-LYASE MESSENGER-RNA AND CHALCONE SYNTHASE MESSENGER-RNA IN PEA EPICOTYLS INDUCED BY ELICITOR FROM MYCOSPHAERELLA-PINODES

H YOSHIOKA, T SHIRAISHI, S KAWAMATA, K NASU, T YAMADA, Y ICHINOSE, H OKU

PLANT AND CELL PHYSIOLOGY 33 ( 2 ) 201 - 204 1992.3

Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Orthovanadate delayed accumulation of mRNAs encoding phenylalanine ammonia-lyase and chalcone synthase in pea epicotyls induced by an elicitor from Mycosphaerella pinodes. However, accumulation of mRNA for a putative P-type ATPase was not affected. The relationship between the ATPase and defense responses is discussed.

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Molecular cloning of phenylalanine ammonia-lyase cDNA from Pisum sativum 共著

Plant Molecular Biology 20,167-170 1992

Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthovanadate and a suppressor from Mycosphae-rella pinodes 共著

Annals of the Phytopathological Society of JAPAN 58,405-410 1992

Suppression of the Activation of Chitinase and β-1,3-Glucanase in Pea Epicotyls by Orthovanadate and a Suppressor from Mycosphaerella pinodes

YOSHIOKA Hirofumi, SHIRAISHI Tomonori, NASU Kimio, YAMADA Tetsuji, ICHINOSE Yuki, OKU Hachiro

Annals of the Phytopathological Society of Japan 58,405-410 ( 3 ) 405 - 410 1992

Language：English Publisher：The Phytopathological Society of Japan (PSJ)

A pea pathogen, Mycosphaerella Pinodes, secretes both an elicitor and a suppressor in its pycnospore germination fluid. The effects of the elicitor, the suppressor and orthovanadate, an inhibitor of P-type ATPase, on the activation of pathogenesis-related proteins such as enclochitinase and β-1,3-glucanase were examined. The elicitor induced the activation of these enzymes in pea, soybean and kidney bean. Such activation in 3 plant species tested was suppressed by the concomitant presence of 1 mM orthovanadate with the elicitor. The suppressor, however, suppressed the activation in only pea plants but did not in nonhost plants of M. pinodes. These results suggest that the inhibition of the ATPase in plasma membranes highly correlates to the suppression of the activation of endo-chitinase and β-1,3-gltlcanase.

DOI： 10.3186/jjphytopath.58.405

CiNii Article

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Other Link： http://agriknowledge.affrc.go.jp/RN/2010481141

An endogenous suppressor of the defense response in Pisum sativum 共著

Plant Cell Physiology 33,617-626 1992

Endogenous elicitor present in barley seeds II. Mechanisms of the induction of the resistance in barley leaves to Erysiphe graminis 共著

Journal of Phytopathology 135,167-176 1992

A Reporter Gene Expression Under the Control of a Pea Phenylalanine Ammonia Lyase-gene Promoter

Yamada Tetsuji, Hashimoto Tadaaki, Sriprasertsak Permpong

Scientific reports of the Faculty of Agriculture, Okayama University 80,51-60 51 - 60 1992

TWO suppressors, suppresciws A and B secreted by a pea pathogen, Mycosphaerella pinodes 共著

Plant Cell Physiology 33,663-667 1992

TWO suppressors, suppresciws A and B secreted by a pea pathogen, Mycosphaerella pinodes 共著

Plant Cell Physiology 33,663-667 1992

Molecular cloning of chalcone synthase cDNA frow Pisum sativum 共著

Plant Molecular Biology 18,1009-1012 1992

A reporter gene expression under the control of a pea phenylalanine ammonia-lyase gene promoter(共著)

Yamada Tetsuji, Hashimoto Tadaaki, Sriprasertsak Permpong

Scientific Reports of the Faculty of Agriculture Okayama University 80,51-60 51 - 60 1992

Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter. International journal

T Hashimoto, T Yamada, A Tada, S Kawamata, Y Tanaka, P Sriprasertsak, Y Ichinose, H Kato, S Izutsu, T Shiraishi, H Oku, Y Ohtsuki

Plant cell reports 11,183-187 ( 4 ) 183 - 7 1992

Language：English

High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

DOI： 10.1007/BF00232529

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INHIBITION OF ATPASE ACTIVITY IN PEA PLASMA-MEMBRANES INSITU BY A SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

T SHIRAISHI, M ARAKI, H YOSHIOKA, KOBAYASHI, I, T YAMADA, Y ICHINOSE, H KUNOH, H OKU

PLANT AND CELL PHYSIOLOGY 32 ( 7 ) 1067 - 1075 1991.10

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

A pathogenic fungus of pea, Mycosphaerella pinodes, secretes a so-called "suppressor" in its pycnospore germination fluid. The suppressor blocks the defense responses and induces local susceptibility (accessibility) in pea plants to agents that are not pathogenic in pea. The suppressor nonspecifically inhibits the ATPase activity in plasma membranes prepared from pea, soybean, kidney bean, cowpea and barley plants. However, cytochemical studies by electron microscopy indicate that the suppressor specifically inhibits the ATPase in pea cell membranes, but not in those of four other plant species tested. That is, the specificity of the suppressor appears at the cell and/or tissue level, but is not evident in vitro. Furthermore, the inhibitory effect of the suppressor is temporary because the ATPase activity recovers 9 h after the treatment. A similar effect was observed after inoculation with M. pinodes but not with a nonpathogen of pea, M. ligulicola. The role of the suppressor in host-parasite specificity is discussed.

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