Updated on 2025/09/30

写真a

 
ICHINOSE Yuki
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Professor
Position
Professor
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Degree

  • Doctor of Philosophy, Science ( Osaka University )

  • Master of Agriculture ( Okayama University )

Research Interests

  • 植物病理学

  • Plant Pathology

  • Plant Molecular Biology

  • Plant Genetic Engineering

Research Areas

  • Environmental Science/Agriculture Science / Plant protection science

Education

  • Osaka University    

    - 1988

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  • Osaka University   理学研究科   生物化学

    - 1988

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    Country: Japan

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  • Okayama University   大学院農学研究科  

    1983.4 - 1985.3

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  • Okayama University   農学部   農学科

    - 1983

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    Country: Japan

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  • Okayama University    

    - 1983

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Research History

  • - Okayama University, Professor   環境生命科学研究科   Professor

    2018.4

  • - Okayama University, Professor   グローバル人材育成院   Professor

    2017.4 - 2018.3

  • - Okayama University, Professor   環境生命科学研究科   Professor

    2007.4 - 2017.3

  • - Okayama University, Professor   自然科学研究科   Professor

    2005.4 - 2007.3

  • - Okayama University, Professor

    2001

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  • - 岡山大学 教授

    2001

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  • Okayama University   自然科学研究科   Associate Professor (as old post name)

    1999.4 - 2001.3

  • Okayama University

    1989 - 2001

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  • Okayama University

    1989 - 2001

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  • Osaka University

    1989

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  • Osaka University, Research Assistant

    1989

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Professional Memberships

  • 日本植物病理学会

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  • 植物微生物研究会

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  • International Society for Molecular Plant-Microbe Interactions

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Committee Memberships

  • 日本植物病理学会   会長  

    2024.4   

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    Committee type:Academic society

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  • 植物病理学会   副会長  

    2023.4 - 2024.3   

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  • 日本植物病理学会   理事  

    2020.1   

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    Committee type:Academic society

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  • 日本植物病理学会   関西部会部会長  

    2019.4 - 2020.3   

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    Committee type:Academic society

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  •   Plant Cell Physiology編集委員(2005-2007)  

    2005 - 2007   

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Papers

  • Positive chemotaxis to plant apoplastic fluids of Pseudomonas syringae pv. tabaci 6605 and metabolome analysis Reviewed

    Yuta Watanabe, Stephany Angelia Tumewu, Hajime Yamada, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Journal of General Plant Pathology   89 ( 4 )   219 - 223   2023.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Pseudomonas syringae pv. tabaci 6605 (Pta6605) is a causal agent of wildfire disease in host tobacco plants. Although chemotaxis has been shown to be necessary for Pta6605 in tobacco infection, the chemoattractants at the site of infection are unclear. Pta6605 was attracted to the apoplastic fluid from not only host tobacco leaves but also non-host plant leaves, indicating that Pta6605 is attracted to common plant metabolites. Metabolome analysis of apoplastic fluid from tobacco leaves revealed that amino acids including γ-aminobutyric acid and organic acids are abundant, suggesting that these compounds are potential chemoattractants.

    DOI: 10.1007/s10327-023-01126-4

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    Other Link: https://link.springer.com/article/10.1007/s10327-023-01126-4/fulltext.html

  • Requirement of chemotaxis and aerotaxis in host tobacco infection by Pseudomonas syringae pv. tabaci 6605 Reviewed

    Yuki Ichinose, Yuta Watanabe, Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda

    Physiological and Molecular Plant Pathology   124   101970 - 101970   2023.3

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.pmpp.2023.101970

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  • HopAZ1, a type-III effector of Pseudomonas amygdali pv. tabaci, induces a hypersensitive response in tobacco wildfire-resistant Nicotiana tabacum cv. N509. Reviewed

    Kashihara, S., Nishimura, T., Noutoshi, Y., Yamamoto, M., Toyoda, K., Ichinose, Y. and Matsui, H.

    Mol. Plant Pathol.   23 ( 6 )   885 - 894   2022.6

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  • HopAZ1, a type III effector of Pseudomonas amygdali pv. tabaci , induces a hypersensitive response in tobacco wildfire‐resistant Nicotiana tabacum ‘N509’ Reviewed

    Sachi Kashihara, Takafumi Nishimura, Yoshiteru Noutoshi, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Hidenori Matsui

    Molecular Plant Pathology   23 ( 6 )   885 - 894   2022.6

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/mpp.13198

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mpp.13198

  • Identification of aerotaxis receptor proteins involved in host plant infection by Pseudomonas syringae pv. tabaci 6605. Reviewed

    Tumewu, S. A., F., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., Ichinose, Y.

    Microbes and Environments   37   ME21076   2022.2

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1264/jsme2.ME21076

  • Identification of Aerotaxis Receptor Proteins Involved in Host Plant Infection by Pseudomonas syringae pv. tabaci 6605. Reviewed

    Stephany Angelia Tumewu, Yuta Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbes and environments   37 ( 1 )   2022

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    Pseudomonas syringae pv. tabaci 6605 (Pta6605) is a foliar plant pathogen that causes wildfire disease on tobacco plants. It requires chemotaxis to enter plants and establish infection. While chemotactic signals appear to be the main mechanism by which Pta6605 performs directional movement, the involvement of aerotaxis or energy taxis by this foliar pathogen is currently unknown. Based on domain structures and similarity with more than 50 previously identified putative methyl-accepting chemotaxis proteins (MCPs), the genome of Pta6605 encodes three potential aerotaxis transducers. We identified AerA as the main aerotaxis transducer and found that it possesses a taxis-to-serine-and-repellent (Tsr)-like domain structure that supports a periplasmic 4HB-type ligand-binding domain (LBD). The secondary aerotaxis transducer, AerB, possesses a cytosolic PAS-type LBD, similar to the Aer of Escherichia coli and Pseudomonas aeruginosa. Aerotaxis ability by single and double mutant strains of aerA and aerB was weaker than that by wild-type Pta6605. On the other hand, another cytosolic PAS-type LBD containing MCP did not make a major contribution to Pta6605 aerotaxis in our assay system. Furthermore, mutations in aerotaxis transducer genes did not affect surface motility or chemotactic attraction to yeast extract. Single and double mutant strains of aerA and aerB showed less colonization in the early stage of host plant infection and lower biofilm production than wild-type Pta6605. These results demonstrate the presence of aerotaxis transducers and their contribution to host plant infection by Pta6605.

    DOI: 10.1264/jsme2.ME21076

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  • Ralstonia solanacearum type III effector RipAC targets SGT1 to suppress effector-triggered immunity.

    Nakano, M., Ichinose, Y., Mukaihara, T.

    Plant Cell Physiol.   61 ( 12 )   2067 - 2076   2021.12

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  • Role of trehalose synthesis in Ralstonia syzygii subsp. indonesiensis PW1001 in inducing hypersensitive response on eggplant (Solanum melongena cv. Senryo-nigou) Reviewed

    Laili, N, Mukaihara, T, Matsui, H, Yamamoto, M, Noutoshi, Y, Toyoda, K, Ichinose, Y

    The Plant Pathology Journal   37 ( 6 )   566 - 579   2021.12

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Korean Society of Plant Pathology  

    Ralstonia syzygii subsp. indonesiensis (Rsi, former name: Ralstonia solanacearum phylotype IV) PW1001, a causal agent of potato wilt disease, induces hypersensitive response (HR) on its non-host eggplant (Solanum melongena cv. Senryo-nigou). The disaccharide trehalose is involved in abiotic and biotic stress tolerance in many organisms. We found that trehalose is required for eliciting HR on eggplant by plant pathogen Rsi PW1001. In R. solanacearum, it is known that the OtsA/OtsB pathway is the dominant trehalose synthesis pathway, and otsA and otsB encode trehalose-6-phosphate (T6P) synthase and T6P phosphatase, respectively. We generated otsA and otsB mutant strains and found that these mutant strains reduced the bacterial trehalose concentration and HR induction on eggplant leaves compared to wild-type. Trehalose functions intracellularly in Rsi PW1001 because addition of exogenous trehalose did not affect the HR level and ion leakage. Requirement of trehalose in HR induction is not common in R. solanacearum species complex because mutation of otsA in Ralstonia pseudosolanacearum (former name: Ralstonia solanacearum phylotype I) RS1002 did not affect HR on the leaves of its non-host tobacco and wild eggplant Solanum torvum. Further, we also found that each otsA and otsB mutant had reduced ability to grow in a medium containing NaCl and sucrose, indicating that trehalose also has an important role in osmotic stress tolerance.

    DOI: 10.5423/ppj.oa.06.2021.0087

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    Other Link: http://ppjonline.org/journal/view.php?doi=10.5423/PPJ.OA.06.2021.0087

  • Identification of chemoreceptor proteins for amino acids involved in host plant infection in Pseudomonas syringae pv. tabaci 6605 Reviewed

    Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbiological Research   253   126869 - 126869   2021.12

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.micres.2021.126869

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  • Identification of chemoreceptor proteins for amino acids involved in host plant infection in Pseudomonas syringae pv. tabaci 6605. Reviewed

    Microbiol. Res.   253   126869   2021.8

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    DOI: https://doi.org/10.1016/j.micres.2021.126869

  • Complete Genome Sequence of Pseudomonas amygdali pv. tabaci Strain 6605, a Causal Agent of Tobacco Wildfire Disease Reviewed

    Hidenori Matsui, Takafumi Nishimura, Shuta Asai, Sachiko Masuda, Ken Shirasu, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   10 ( 28 )   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/MRA.00405-21

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  • Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 Reviewed

    Keisuke Ogura, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Yuki Ichinose

    Biochemistry and Biophysics Reports   26   100944 - 100944   2021.7

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    DOI: 10.1016/j.bbrep.2021.100944

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  • Cluster II che genes of Pseudomonas syringae pv. tabaci 6605, orthologs of cluster I in Pseudomonas aeruginosa, are required for chemotaxis and virulence Reviewed

    Stephany Angelia Tumewu, Yujiro Ogawa, Takumi Okamoto, Yuka Sugihara, Hajime Yamada, Fumiko Taguchi, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Molecular Genetics and Genomics   2021.1

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00438-020-01745-y

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    Other Link: http://link.springer.com/article/10.1007/s00438-020-01745-y/fulltext.html

  • HopH1 effectors of Pseudomonas syringae pv. tomato DC3000 and pv. syringae B728a induce HR cell death in nonhost eggplant Solanum torvum Reviewed

    Kamrun Nahar, Takafumi Mukaihara, Fumiko Taguchi, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   87 ( 1 )   24 - 29   2021.1

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    DOI: 10.1007/s10327-020-00961-z

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    Other Link: http://link.springer.com/article/10.1007/s10327-020-00961-z/fulltext.html

  • Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 Reviewed

    Yuki Ichinose, Takafumi Nishimura, Minori Harada, Ryota Kashiwagi, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Daigo Takemoto, Hidenori Matsui

    PLANT PATHOLOGY JOURNAL   36 ( 2 )   148 - 156   2020.4

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    DOI: 10.5423/PPJ.OA.11.2019.0273

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  • PsyR, a transcriptional regulator in quorum sensing system, binds lux box-like sequence in psyI promoter without AHL quorum sensing molecule and activates psyI transcription with AHL in Pseudomonas syringae pv. tabaci 6605 Reviewed

    Yuki Ichinose, Yousuke Tasaka, Satoru Yamamoto, Yuko Inoue, Motohiro Takata, Yukiko Nakatsu, Fumiko Taguchi, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Hidenori Matsui

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   124 - 133   2020.3

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    DOI: 10.1007/s10327-019-00893-3

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  • Requirement of γ-Aminobutyric Acid Chemotaxis for Virulence of Pseudomonas syringae pv. tabaci 6605. Reviewed

    Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbes and environments   35 ( 4 )   2020

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    γ-Aminobutyric acid (GABA) is a widely distributed non-proteinogenic amino acid that accumulates in plants under biotic and abiotic stress conditions. Recent studies suggested that GABA also functions as an intracellular signaling molecule in plants and in signals mediating interactions between plants and phytopathogenic bacteria. However, the molecular mechanisms underlying GABA responses to bacterial pathogens remain unknown. In the present study, a GABA receptor, named McpG, was conserved in the highly motile plant-pathogenic bacteria Pseudomonas syringae pv. tabaci 6605 (Pta6605). We generated a deletion mutant of McpG to further investigate its involvement in GABA chemotaxis using quantitative capillary and qualitative plate assays. The wild-type strain of Pta6605 was attracted to GABA, while the ΔmcpG mutant abolished chemotaxis to 10‍ ‍mM GABA. However, ΔmcpG retained chemotaxis to proteinogenic amino acids and succinic semialdehyde, a structural analog of GABA. Furthermore, ΔmcpG was unable to effectively induce disease on host tobacco plants in three plant inoculation assays: flood, dip, and infiltration inoculations. These results revealed that the GABA sensing of Pta6605 is important for the interaction of Pta6605 with its host tobacco plant.

    DOI: 10.1264/jsme2.ME20114

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  • A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis Reviewed

    Lei Zhao, Le Thi Phuong, Mai Thanh Luan, Aprilia Nur Fitrianti, Hidenori Matsui, Hirofumi Nakagami, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   85 ( 6 )   405 - 412   2019.11

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    DOI: 10.1007/s10327-019-00863-9

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  • Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides Reviewed International journal

    Pierre Buscaill, Balakumaran Chandrasekar, Nattapong Sanguankiattichai, Jiorgos Kourelis, Farnusch Kaschani, Emma L. Thomas, Kyoko Morimoto, Markus Kaiser, Gail M. Preston, Yuki Ichinose, Renier A. L. van der Hoorn

    Science   364 ( 6436 )   2019.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Association for the Advancement of Science (AAAS)  

    Plants and animals recognize conserved flagellin fragments as a signature of bacterial invasion. These immunogenic elicitor peptides are embedded in the flagellin polymer and require hydrolytic release before they can activate cell surface receptors. Although much of flagellin signaling is understood, little is known about the release of immunogenic fragments. We discovered that plant-secreted β-galactosidase 1 (BGAL1) of Nicotiana benthamiana promotes hydrolytic elicitor release and acts in immunity against pathogenic Pseudomonas syringae strains only when they carry a terminal modified viosamine (mVio) in the flagellin O-glycan. In counter defense, P. syringae pathovars evade host immunity by using BGAL1-resistant O-glycans or by producing a BGAL1 inhibitor. Polymorphic glycans on flagella are common to plant and animal pathogenic bacteria and represent an important determinant of host immunity to bacterial pathogens.

    DOI: 10.1126/science.aav0748

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  • Loliolide, a Carotenoid Metabolite, Is a Potential Endogenous Inducer of Herbivore Resistance. Reviewed International journal

    Mika Murata, Yusuke Nakai, Kei Kawazu, Masumi Ishizaka, Hideyuki Kajiwara, Hiroshi Abe, Kasumi Takeuchi, Yuki Ichinose, Ichiro Mitsuhara, Atsushi Mochizuki, Shigemi Seo

    Plant physiology   179 ( 4 )   1822 - 1833   2019.4

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    Jasmonic acid (JA) plays an important role in the induction of herbivore resistance in many plants. However, JA-independent herbivore resistance has been suggested. An herbivore-resistance-inducing substance was isolated from Tobacco mosaic virus-infected tobacco (Nicotiana tabacum) leaves in which a hypersensitive response (HR) was induced and identified as loliolide, which has been identified as a β-carotene metabolite. When applied to tomato (Solanum lycopersicum) leaves, loliolide decreased the survival rate of the two-spotted spider mite, Tetranychus urticae, egg deposition by the same pest, and the survival rate of larvae of the common cutworm Spodoptera litura without exhibiting toxicity against these herbivores. Endogenous loliolide levels increased not only with an infestation by Slitura larvae, but also with the exogenous application of their oral secretions in tomato. A microarray analysis identified cell-wall-associated defense genes as loliolide-responsive tomato genes, and exogenous JA application did not induce the expression of these genes. Suppressor of zeaxanthin-less (szl), an Arabidopsis (Arabidopsis thaliana) mutant with a point mutation in a key gene of the β-carotene metabolic pathway, exhibited the decreased accumulation of endogenous loliolide and increased susceptibility to infestation by the western flower thrip (Frankliniella occidentalis). A pretreatment with loliolide decreased susceptibility to thrips in the JA-insensitive Arabidopsis mutant coronatine-insensitive1 Exogenous loliolide did not restore reduced electrolyte leakage in szl in response to a HR-inducing bacterial strain. These results suggest that loliolide functions as an endogenous signal that mediates defense responses to herbivores, possibly independently of JA, at least in tomato and Arabidopsis plants.

    DOI: 10.1104/pp.18.00837

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  • Quorum-dependent expression of rsmX and rsmY, small non-coding RNAs, in Pseudomonas syringae Reviewed

    Nakatsu, Y, Matsui, H, Yamamoto, M, Noutoshi, Y, Toyoda, K, Ichinose, Y

    Microbiological Research   223–225   72 - 78   2019.4

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    DOI: 10.1016/j.micres.2019.04.004

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  • MexEF-OprN multidrug efflux pump transporter negatively controls N-acyl-homoserine lactone accumulation in pseudomonas syringae pv. Tabaci 6605. Reviewed

    Sawada T, Eguchi M, Asaki S, Kashiwagi R, Shimomura K, Taguchi F, Matsui H, Yamamoto M, Noutoshi Y, Toyoda K, Ichinose Y

    Molecular genetics and genomics : MGG   293 ( 4 )   907 - 917   2018.8

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    DOI: 10.1007/s00438-018-1430-9

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  • Pseudomonas syringae pv. tomato OxyR Is Required for Virulence in Tomato and Arabidopsis Reviewed

    Yasuhiro Ishiga, Yuki Ichinose

    MOLECULAR PLANT-MICROBE INTERACTIONS   29 ( 2 )   119 - 131   2016.2

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    DOI: 10.1094/MPMI-09-15-0204-R

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  • Motility-mediated regulation of virulence in Pseudomonas syringae Invited Reviewed

    Yuki Ichinose, Takahiro Sawada, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Fumiko Taguchi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   95   50 - 54   2016

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    DOI: 10.1016/j.pmpp.2016.02.005

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  • Characterization of quorum sensing-controlled transcriptional regulator MarR and Rieske (2Fe-2S) cluster-containing protein (Orf5) that are involved in resistance to environmental stresses in Pseudomonas syringae pv. tabaci 6605. Reviewed International journal

    Taguchi F, Inoue Y, Suzuki T, Inagaki Y, Yamamoto M, Yoyoda K, Noutoshi Y, Shiraishi T, Ichinose Y

    Mol. Plant Pathol.   16 ( 4 )   376 - 387   2015

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    Pseudomonas syringae pv. tabaci 6605 (Pta6605) produces acyl homoserine lactones (AHLs), quorum sensing (QS) molecules that are indispensable for virulence in host tobacco infection. Genome-wide transcriptional profiling of several QS-defective mutants revealed that the expression of the genes encoding the MarR family transcriptional regulator (MarR) and a Rieske 2Fe-2S cluster-containing protein (Orf5) located adjacent to psyI, a gene encoding AHL synthetase, are significantly repressed. Exogenous application of AHL recovered the expression of both marR and orf5 genes in the ΔpsyI mutant, indicating that AHL positively regulates the expression of these genes. To investigate the role of these genes in the virulence of Pta6605, ΔmarR and Δorf5 mutants were generated. Both mutants showed decreased swimming and swarming motilities, decreased survival ability under oxidative and nitrosative stresses and, consequently, reduced virulence on host tobacco plants. Transmission electron micrographs showed that the structure of the cell membranes of ΔmarR and Δorf5 mutants was severely damaged. Furthermore, not only the ratio of dead cells, but also the amount of flagella, extracellular DNA and protein released into the culture supernatant, was significantly increased in both mutants, indicating that the disruption of marR and orf5 genes might induce structural changes in the membrane and cell lysis. Because both mutants showed partly similar expression profiles, both gene products might be involved in the same regulatory cascades that are required for QS-dependent survival under environmentally stressed conditions.

    DOI: 10.1111/mpp.12187

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  • Ralstonia solanacearum type III secretion system effector Rip36 induces hypersensitive response in the nonhost wild eggplant Solanum torvum. Reviewed

    Nahar K, Matsumoto, I, Taguchi, F, Inagaki, Y, Yamamoto, M, Yoyoda, K, Shiraishi, T, Ichinose, Y, Mukaihara, T

    Mol. Plant Pathol.   15 ( (3) )   297 - 303   2014

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    DOI: 10.1111/mpp.12079

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  • Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility Reviewed

    Christopher R. Clarke, Delphine Chinchilla, Sarah R. Hind, Fumiko Taguchi, Ryuji Miki, Yuki Ichinose, Gregory B. Martin, Scotland Leman, Georg Felix, Boris A. Vinatzer

    NEW PHYTOLOGIST   200 ( 3 )   847 - 860   2013.11

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    DOI: 10.1111/nph.12408

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  • Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria Reviewed

    Yuki Ichinose, Fumiko Taguchi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tatsuo Atsumi, Tatsuo Atsumi, Masako Iwaki, Hiromi Manabe, Mio Kumagai, Quan Thanh Nguyen, Chi Linh Nguyen, Yoshishige Inagaki, Hiroshi Ono, Kazuhiro Chiku, Tadashi Ishii, Mitsuru Yoshida

    Journal of General Plant Pathology   79 ( 5 )   359 - 365   2013.9

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    DOI: 10.1007/s10327-013-0464-4

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  • Comparative analysis of flagellin glycans among pathovars of phytopathogenic Pseudomonas syringae. Reviewed

    Chiku K, Yamamoto M, Ohnishi-Kameyama M, Ishii T, Yoshida M, Taguchi F, Ichinose Y, Ono H

    Carbohydrate research   375   100 - 104   2013.6

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    DOI: 10.1016/j.carres.2013.04.018

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  • Virulence factor regulator (Vfr) controls virulence-associated phenotypes in Pseudomonas syringae pv. tabaci 6605 by a quorum sensing-independent mechanism Reviewed

    Fumiko Taguchi, Yuki Ichinose

    Molecular Plant Pathology   14 ( 3 )   279 - 292   2013.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/mpp.12003

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  • Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and required for surface motility and virulence Reviewed

    Linh Chi Nguyen, Fumiko Taguchi, Quang Minh Tran, Kana Naito, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Hiroshi Ono, Mitsuru Yoshida, Kazuhiro Chiku, Tadashi Ishii, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Molecular Plant Pathology   13 ( 7 )   764 - 774   2012.9

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    Type IV pilin (PilA) is a major constituent of pilus and is required for bacterial biofilm formation, surface motility and virulence. It is known that mature PilA is produced by cleavage of the short leader sequence of the pilin precursor, followed by methylation of N-terminal phenylalanine. The molecular mass of the PilA mature protein from the tobacco bacterial pathogen Pseudomonas syringae pv. tabaci 6605 (Pta 6605) has been predicted to be 12 329 Da from its deduced amino acid sequence. Previously, we have detected PilA as an approximately 13-kDa protein by immunoblot analysis with anti-PilA-specific antibody. In addition, we found the putative oligosaccharide-transferase gene tfpO downstream of pilA. These findings suggest that PilA in Pta 6605 is glycosylated. The defective mutant of tfpO (ΔtfpO) shows reductions in pilin molecular mass, surface motility and virulence towards host tobacco plants. Thus, pilin glycan plays important roles in bacterial motility and virulence. The genetic region around pilA was compared among P. syringae pathovars. The tfpO gene exists in some strains of pathovars tabaci, syringae, lachrymans, mori, actinidiae, maculicola and P. savastanoi pv. savastanoi. However, some strains of pathovars tabaci, syringae, glycinea, tomato, aesculi and oryzae do not possess tfpO, and the existence of tfpO is independent of the classification of pathovars/strains in P. syringae. Interestingly, the PilA amino acid sequences in tfpO-possessing strains show higher homology with each other than with tfpO-nonpossessing strains. These results suggest that tfpO and pilA might co-evolve in certain specific bacterial strains.

    DOI: 10.1111/j.1364-3703.2012.00789.x

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  • Identification of Genes Involved in the Glycosylation of Modified Viosamine of Flagellins in Pseudomonas syringae by Mass Spectrometry Reviewed

    Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Chi L. Nguyen, Fumiko Taguchi, Kazuhiro Chiku, Tadashi Ishii, Hiroshi Ono, Mitsuru Yoshida, Yuki Ichinose

    GENES   2 ( 4 )   788 - 803   2011.12

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    DOI: 10.3390/genes2040788

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  • Role of Type IV Pili in Virulence of Pseudomonas syringae pv. tabaci 6605: Correlation of Motility, Multidrug Resistance, and HR-Inducing Activity on a Nonhost Plant Reviewed

    Fumiko Taguchi, Yuki Ichinose

    MOLECULAR PLANT-MICROBE INTERACTIONS   24 ( 9 )   1001 - 1011   2011.9

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    DOI: 10.1094/MPMI-02-11-0026

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  • Two flagellar stators and their roles in motility and virulence in Pseudomonas syringae pv. tabaci 6605. Reviewed

    Kanda E, Tatsuta T, Suzuki T, Taguchi F, Naito K, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y

    Mol. Genet. Genomics   285 ( (2) )   163 - 174   2011

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    DOI: 10.1007/s00438-010-0594-8

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  • Degeneration of hrpZ gene in Pseudomonas syringae pv. tabaci to evade tobacco defence: an arms race between tobacco and its bacterial pathogen. Reviewed

    Tsunemi K, Taguchi F, Marutani M, Watanabe-Sugimoto M, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y

    Mol Plant Pathol.   12 ( (7) )   709 - 714   2011

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    DOI: 10.1111/j.1364-3703.2011.00705.x

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  • Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605. Reviewed

    Taguchi F, Yamamoto M, Ohnishi-Kameyama M, Iwaki M, Yoshida M, Ishii T, Konishi T, Ichinose Y

    Microbiology (Reading, England)   156 ( Pt 1 )   72 - 80   2010.1

  • Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics Reviewed

    Koh Aoki, Kentaro Yano, Ayako Suzuki, Shingo Kawamura, Nozomu Sakurai, Kunihiro Suda, Atsushi Kurabayashi, Tatsuya Suzuki, Taneaki Tsugane, Manabu Watanabe, Kazuhide Ooga, Maiko Torii, Takanori Narita, Tadasu Shin-i, Yuji Kohara, Naoki Yamamoto, Hideki Takahashi, Yuichiro Watanabe, Mayumi Egusa, Motoichiro Kodama, Yuki Ichinose, Mari Kikuchi, Sumire Fukushima, Akiko Okabe, Tsutomu Arie, Yuko Sato, Katsumi Yazawa, Shinobu Satoh, Toshikazu Omura, Hiroshi Ezura, Daisuke Shibata

    BMC Genomics   11 ( 1 )   210 - 210   2010

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    DOI: 10.1186/1471-2164-11-210

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  • Genetic analysis of genes involved in synthesis of modified 4-amino-4,6-dideoxyglucose in flagellin of Pseudomonas syringae pv. tabaci Reviewed

    Linh Chi Nguyen, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Salamah Andi, Fumiko Taguchi, Masako Iwaki, Mitsuru Yoshida, Tadashi Ishii, Tomoyuki Konishi, Kazuhiko Tsunemi, Yuki Ichinose

    Molecular Genetics and Genomics   282   595 - 605   2009.12

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    DOI: 10.1007/s00438-009-0489-8

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  • Glycosylation of flagellin from Pseudomonas syringae pv. tabaci 6605 contributes to evasion of host tobacco plant surveillance system. Reviewed

    Taguchi F, Suzuki T, Takeuchi K, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y

    Physiol. Mol. Plant Pathol.   74 ( 1 )   11 - 17   2009

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    DOI: 10.1016/j.pmpp.2009.08.001

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  • Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Reviewed International journal

    Kasumi Takeuchi, Hiroshi Ono, Mitsuru Yoshida, Tadashi Ishii, Etsuko Katoh, Fumiko Taguchi, Ryuji Miki, Katsuyoshi Murata, Hanae Kaku, Yuki Ichinose

    Journal of bacteriology   189 ( 19 )   6945 - 56   2007.10

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    DOI: 10.1128/JB.00500-07

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  • Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci. Reviewed International journal

    Fumiko Taguchi, Kasumi Takeuchi, Etsuko Katoh, Katsuyoshi Murata, Tomoko Suzuki, Mizuri Marutani, Takayuki Kawasaki, Minako Eguchi, Shizue Katoh, Hanae Kaku, Chihiro Yasuda, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Cellular microbiology   8 ( 6 )   923 - 38   2006.6

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    DOI: 10.1111/j.1462-5822.2005.0674.x

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  • Flagellin glycosylation island in Pseudomonas syringae pv. glycinea and its role in host specificity. Reviewed International journal

    Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of bacteriology   185 ( 22 )   6658 - 65   2003.11

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    DOI: 10.1128/JB.22.6658-6665.2003

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  • Flagella defective mutant fliD of Pseudomonas syringae pv. tabaci that secretes monomer flagellin induces strong HR on nonhost tomato cells. Reviewed

    Shimizu R, Taguchi F, Marutani M, Mukaihara T, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y

    Mol.Genet. Genomics   269 ( 1 )   21 - 30   2003

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    DOI: 10.1007/s00438-003-0817-3

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  • Pseudomonas syringae pv. tabaci 6605 Requires Seven Type III Effectors to Infect Nicotiana benthamiana. Reviewed International journal

    Kana Kuroe, Takafumi Nishimura, Sachi Kashihara, Nanami Sakata, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose, Hidenori Matsui

    Molecular plant pathology   26 ( 5 )   e70091   2025.5

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    Type III effectors (T3Es), virulence factors injected into plant cells via the type III secretion system (T3SS), play essential roles in the infection of host plants. Pseudomonas syringae pv. tabaci 6605 (Pta 6605) is the causal agent of wildfire disease in tobacco and harbours at least 22 T3Es in its genome. However, the specific T3Es required by Pta 6605 to infect Nicotiana benthamiana remain unidentified. In this study, we investigated the T3Es that contribute to Pta 6605 infection of N. benthamiana. We constructed Pta 6605 poly-T3E-deficient mutants (Pta DxE) and inoculated them into N. benthamiana. Flood assay, which mimics natural opening-based entry, showed that mutant strains lacking 14-22 T3Es, namely, Pta D14E-D22E mutants, exhibited reduced disease symptoms. By contrast, infiltration inoculation, which involves direct injection into leaves, showed that the Pta D14E to Pta D20E mutants developed disease symptoms. Notably, the Pta D20E, containing AvrE1 and HopM1, induced weak but observable symptoms upon infiltration inoculation. Conversely, no symptoms were observed in either the flood assay or infiltration inoculation for Pta D21E and Pta D22E. Taken together, these findings indicate that the many T3Es such as AvrPto4/AvrPtoB, HopW1/HopAE1, and HopM1/AvrE1 in Pta 6605 collectively contribute to invasion through natural openings and symptom development in N. benthamiana. This study provides the basis for understanding virulence in the host by identifying the minimum T3E repertoire required by Pta 6605 to infect N. benthamiana.

    DOI: 10.1111/mpp.70091

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  • Genomic Islands of Pseudomonas syringae pv. tabaci 6605: Identification of PtaGI-1 as a Pathogenicity Island With Effector Genes and a Tabtoxin Cluster. Reviewed International journal

    Yuta Watanabe, Kotomi Kunishi, Hidenori Matsui, Nanami Sakata, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Molecular plant pathology   26 ( 5 )   e70087   2025.5

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    Genomic islands (GIs) are 20-500 kb DNA regions that are thought to be acquired by horizontal gene transfer. GIs that confer pathogenicity and environmental adaptation have been reported in Pseudomonas species; however, GIs that enhance bacterial virulence have not. Here, we identified 110 kb and 103 kb GIs in P. syringae pv. tabaci 6605 (Pta6605), the causative agent of tobacco wildfire disease, which has the ability to produce tabtoxin as a phytotoxin. These GIs are partially homologous to known genomic islands in Pseudomonas aeruginosa and P. syringae pv. phaseolicola and were designated PtaGI-1 and PtaGI-2. Both PtaGIs conserve core genes, whereas each GI possesses different accessory genes. PtaGI-1 contains a tabtoxin biosynthetic gene cluster and three type III effector genes among its accessory genes, whereas PtaGI-2 also contains homologous genes to hsvABC, pathogenicity-related genes in Erwinia amylovora. Inoculation revealed that the PtaGI-1 mutant, but not the PtaGI-2 mutant, lost the ability to biosynthesise tabtoxin and to cause disease. Therefore, PtaGI-1 is thought to be a pathogenicity island. Both PtaGI-1 and PtaGI-2 have a pseudogene of tRNALys on the left border and an intact tRNALys gene on the right border. In a colony of Pta6605, both GIs can be excised at tRNALys, and PtaGI-1 and PtaGI-2 exist in a circular form. These results indicate that tabtoxin biosynthesis genes in PtaGI-1 are required for disease development, and PtaGI-1 is necessary for Pta6605 virulence.

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  • Distinct Infection Mechanisms of Rhizoctonia solani AG-1 IA and AG-4 HG-I+II in Brachypodium distachyon and Barley Reviewed

    Niranjan Mahadevan, Rozi Fernanda, Yusuke Kouzai, Natsuka Kohno, Reiko Nagao, Khin Thida Nyein, Megumi Watanabe, Nanami Sakata, Hidenori Matsui, Kazuhiro Toyoda, Yuki Ichinose, Keiichi Mochida, Hiroshi Hisano, Yoshiteru Noutoshi

    Life   15 ( 2 )   235 - 235   2025.2

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    Rhizoctonia solani is a basidiomycete phytopathogenic fungus that causes rapid necrosis in a wide range of crop species, leading to substantial agricultural losses worldwide. The species complex is divided into 13 anastomosis groups (AGs) based on hyphal fusion compatibility and further subdivided by culture morphology. While R. solani classifications were shown to be independent of host specificity, it remains unclear whether different R. solani isolates share similar virulence mechanisms. Here, we investigated the infectivity of Japanese R. solani isolates on Brachypodium distachyon and barley. Two isolates, AG-1 IA (from rice) and AG-4 HG-I+II (from cauliflower), infected leaves of both plants, but only AG-4 HG-I+II infected roots. B. distachyon accessions Bd3-1 and Gaz-4 and barley cultivar ‘Morex’ exhibited enhanced resistance to both isolates compared to B. distachyon Bd21 and barley cultivars ‘Haruna Nijo’ and ‘Golden Promise’. During AG-1 IA infection, but not AG-4 HG-I+II infection, resistant Bd3-1 and Morex induced genes for salicylic acid (SA) and N-hydroxypipecolic acid (NHP) biosynthesis. Pretreatment with SA or NHP conferred resistance to AG-1 IA, but not AG-4 HG-I+II, in susceptible B. distachyon Bd21 and barley Haruna Nijo. On the leaves of susceptible Bd21 and Haruna Nijo, AG-1 IA developed extensive mycelial networks with numerous infection cushions, which are specialized infection structures well-characterized in rice sheath blight. In contrast, AG-4 HG-I+II formed dispersed mycelial masses associated with underlying necrosis. We propose that the R. solani species complex encompasses at least two distinct infection strategies: AG-1 IA exhibits a hemibiotrophic lifestyle, while AG-4 HG-I+II follows a predominantly necrotrophic strategy.

    DOI: 10.3390/life15020235

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  • Role of Formate Chemoreceptor in Pseudomonas syringae pv. tabaci 6605 in Tobacco Infection. Reviewed

    Phuoc Quy Thang Nguyen, Yuta Watanabe, Hidenori Matsui, Nanami Sakata, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbes and environments   40 ( 3 )   2025

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    Chemotaxis is essential for infection by plant pathogenic bacteria. The causal agent of tobacco wildfire disease, Pseudomonas syringae pv. tabaci 6605 (Pta6605), is known to cause severe leaf disease and is highly motile. The requirement of chemotaxis for infection has been demonstrated through the inoculation of mutant strains lacking chemotaxis sensory component proteins. Pta6605 possesses 54 genes that encode chemoreceptors (known as methyl-accepting chemotaxis proteins, MCPs). Chemoreceptors are classified into several groups based on the type and localization of ligand-binding domains (LBD). Cache LBD-type chemoreceptors have been reported to recognize formate in several bacterial species. In the present study, we identified Cache_3 Cache_2 LBD-type Mcp26 encoded by Pta6605_RS00335 as a chemoreceptor for formate using a quantitative capillary assay, and named it McpF. Although the deletion mutant of mcpF (ΔmcpF) retained attraction to 1% yeast extract, its chemotactic response to formate was markedly reduced. Swimming and swarming motilities were also impaired in the mutant. To investigate the effects of McpF on bacterial virulence, we conducted inoculations on tobacco plants using several methods. The ΔmcpF mutant exhibited weaker virulence in flood and spray assays than wild-type and complemented strains, highlighting not only the involvement of McpF in formate recognition, but also its critical role in leaf entry during the early stages of infection.

    DOI: 10.1264/jsme2.ME25019

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  • Nutrient requirements shape the preferential habitat of Allorhizobium vitis VAR03-1, a commensal bacterium, in the rhizosphere of Arabidopsis thaliana Reviewed

    Niarsi Merry Hemelda, Jiyuan Bao, Megumi Watanabe, Hidenori Matsui, Kazuhiro Toyoda, Yuki Ichinose, Yoshiteru Noutoshi

    Plant And Cell Physiology   2024.8

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    Abstract

    A diverse range of commensal bacteria inhabit the rhizosphere, influencing host plant growth and responses to biotic and abiotic stresses. While root-released nutrients can define soil microbial habitats, the bacterial factors involved in plant-microbe interactions are not well characterized. In this study, we investigated the colonization patterns of two plant disease biocontrol agents, Allorhizobium vitis VAR03-1 and Pseudomonas protegens Cab57, in the rhizosphere of Arabidopsis thaliana using Murashige and Skoog (MS) agar medium. VAR03-1 formed colonies even at a distance from the roots, preferentially in the upper part, while Cab57 colonized only the root surface. The addition of sucrose to the agar medium resulted in excessive proliferation of VAR03-1, similar to its pattern without sucrose, whereas Cab57 formed colonies only near the root surface. Overgrowth of both bacterial strains upon nutrient supplementation inhibited host growth, independent of plant immune responses. This inhibition was reduced in the VAR03-1 ΔrecA mutant, which exhibited increased biofilm formation, suggesting that some activities associated with the free-living lifestyle rather than the sessile lifestyle may be detrimental to host growth. VAR03-1 grew in liquid MS medium with sucrose alone, while Cab57 required both sucrose and organic acids. Supplementation of sugars and organic acids allowed both bacterial strains to grow near and away from Arabidopsis roots in MS agar. These results suggest that nutrient requirements for bacterial growth may determine their growth habitats in the rhizosphere, with nutrients released in root exudates potentially acting as a limiting factor in harnessing microbiota.

    DOI: 10.1093/pcp/pcae091

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  • Rhizoviticin is an alphaproteobacterial tailocin that mediates biocontrol of grapevine crown gall disease Reviewed

    Tomoya Ishii, Natsuki Tsuchida, Niarsi Merry Hemelda, Kirara Saito, Jiyuan Bao, Megumi Watanabe, Atsushi Toyoda, Takehiro Matsubara, Mayuko Sato, Kiminori Toyooka, Nobuaki Ishihama, Ken Shirasu, Hidenori Matsui, Kazuhiro Toyoda, Yuki Ichinose, Tetsuya Hayashi, Akira Kawaguchi, Yoshiteru Noutoshi

    The ISME Journal   18 ( 1 )   2024.1

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    Tailocins are headless phage tail structures that mediate interbacterial antagonism. Although the prototypical tailocins, R- and F-pyocins, in Pseudomonas aeruginosa, and other predominantly R-type tailocins have been studied, their presence in Alphaproteobacteria remains unexplored. Here, we report the first alphaproteobacterial F-type tailocin, named rhizoviticin, as a determinant of the biocontrol activity of Allorhizobium vitis VAR03-1 against crown gall. Rhizoviticin is encoded by a chimeric prophage genome, one providing transcriptional regulators and the other contributing to tail formation and cell lysis, but lacking head formation genes. The rhizoviticin genome retains a nearly intact early phage region containing an integrase remnant and replication-related genes critical for downstream gene transcription, suggesting an ongoing transition of this locus from a prophage to a tailocin-coding region. Rhizoviticin is responsible for the most antagonistic activity in VAR03-1 culture supernatant against pathogenic A. vitis strain, and rhizoviticin deficiency resulted in a significant reduction in the antitumorigenic activity in planta. We identified the rhizoviticin-coding locus in eight additional A. vitis strains from diverse geographical locations, highlighting a unique survival strategy of certain Rhizobiales bacteria in the rhizosphere. These findings advance our understanding of the evolutionary dynamics of tailocins and provide a scientific foundation for employing rhizoviticin-producing strains in plant disease control.

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  • HexR Transcription Factor Contributes to Pseudomonas cannabina pv. alisalensis Virulence by Coordinating Type Three Secretion System Genes Reviewed

    Nanami Sakata, Takashi Fujikawa, Ayaka Uke, Takako Ishiga, Yuki Ichinose, Yasuhiro Ishiga

    Microorganisms   2023.4

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    DOI: 10.3390/microorganisms11041025

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  • CEP peptide, a family of conserved, secreted small peptides acts as an endogenous suppressor in Arabidopsis Reviewed

    Toyoda, K, Fitrianti, A.N, Itoh, C, Hasegawa, H, Matsui, H, Noutoshi, Y, Yamamoto, M, Ichinose, Y, Shiraishi, T

    125   102019   2023.4

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  • Time-series transcriptome of Brachypodium distachyon during bacterial flagellin-induced pattern-triggered immunity Reviewed

    Tsubasa Ogasahara, Yusuke Kouzai, Megumi Watanabe, Akihiro Takahashi, Kotaro Takahagi, June-Sik Kim, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Keiichi Mochida, Yoshiteru Noutoshi

    Frontiers in Plant Science   13   2022.9

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    <jats:p>Plants protect themselves from microorganisms by inducing pattern-triggered immunity (PTI) <jats:italic>via</jats:italic> recognizing microbe-associated molecular patterns (MAMPs), conserved across many microbes. Although the MAMP perception mechanism and initial events during PTI have been well-characterized, knowledge of the transcriptomic changes in plants, especially monocots, is limited during the intermediate and terminal stages of PTI. Here, we report a time-series high-resolution RNA-sequencing (RNA-seq) analysis during PTI in the leaf disks of <jats:italic>Brachypodium distachyon</jats:italic>. We identified 6,039 differentially expressed genes (DEGs) in leaves sampled at 0, 0.5, 1, 3, 6, and 12 hours after treatment (hat) with the bacterial flagellin peptide flg22. The k-means clustering method classified these DEGs into 10 clusters (6 upregulated and 4 downregulated). Based on the results, we selected 10 PTI marker genes in <jats:italic>B. distachyon</jats:italic>. Gene ontology (GO) analysis suggested a tradeoff between defense responses and photosynthesis during PTI. The data indicated the recovery of photosynthesis started at least at 12 hat. Over-representation analysis of transcription factor genes and cis-regulatory elements in DEG promoters implied the contribution of 12 WRKY transcription factors in plant defense at the early stage of PTI induction.</jats:p>

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  • CEP peptide induces susceptibility of Arabidopsis thaliana to non-adapted pathogens. Reviewed

    Fitrianti, A. N., Mai, T. L., Phuong, L. T., Monden, H., Shiiba, N., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T. and Toyoda, K.

    J. Gen. Plant Pathol.   88 ( 5 )   287 - 292   2022.9

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    DOI: https://doi.org/10.1007/s10327-022-01077-2

  • CEP peptide induces susceptibility of Arabidopsis thaliana to non-adapted pathogens Reviewed

    Aprilia Nur Fitrianti, Thanh Luan Mai, Le Thi Phuong, Hiyori Monden, Norika Shiiba, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    Journal of General Plant Pathology   88 ( 5 )   287 - 292   2022.9

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  • Surveillance of pathogenicity of Rhizoctonia solani Japanese isolates with varied anastomosis groups and subgroups on Arabidopsis thaliana. Reviewed

    Abdelghany, M. M. A., Kurikawa, M., Watanabe, M., Yamamoto, M., Matsui, H., Ichinose, Y., Toyoda, K., Kouzai, Y. and Noutoshi, Y.

    Life   12   76   2022.2

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  • Surveillance of Pathogenicity of Rhizoctonia solani Japanese Isolates with Varied Anastomosis Groups and Subgroups on Arabidopsis thaliana Reviewed

    Mai Mohsen Ahmed Abdelghany, Maria Kurikawa, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Yusuke Kouzai, Yoshiteru Noutoshi

    Life   12 ( 1 )   76 - 76   2022.1

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    Rhizoctonia solani is a necrotrophic plant pathogen with a wide host range. R. solani is a species complex consisting of thirteen anastomosis groups (AGs) defined by compatibility of hyphal fusion reaction and subgroups based on cultural morphology. The relationship between such classifications and host specificity remains elusive. Here, we investigated the pathogenicity of seventeen R. solani isolates (AG-1 to 7) in Japan towards Arabidopsis thaliana using leaf and soil inoculations. The tested AGs, except AG-3 and AG-6, induced symptoms in both methods with variations in pathogenicity. The virulence levels differed even within the same AG and subgroup. Some isolates showed tissue-specific infection behavior. Thus, the AGs and their subgroups are suggested to be not enough to define the virulence (host and tissue specificity) of R. solani. We also evaluated the virulence of the isolates on Arabidopsis plants pretreated with salicylic acid, jasmonic acid, and ethylene. No obvious effects were detected on the symptom formation by the virulence isolates, but ethylene and salicylic acid slightly enhanced the susceptibility to the weak and nonvirulent isolates. R. solani seems to be able to overcome the induced defense by these phytohormones in the infection to Arabidopsis.

    DOI: 10.3390/life12010076

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  • Identification of effector candidate genes of Rhizoctonia solani AG-1 IA expressed during infection in Brachypodium distachyon Reviewed

    Sobhy S. H. Abdelsalam, Yusuke Kouzai, Megumi Watanabe, Komaki Inoue, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Seiji Tsuge, Keiichi Mochida, Yoshiteru Noutoshi

    Scientific Reports   10 ( 1 )   2020.12

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>
    <italic>Rhizoctonia solani</italic> is a necrotrophic phytopathogen belonging to basidiomycetes. It causes rice sheath blight which inflicts serious damage in rice production. The infection strategy of this pathogen remains unclear. We previously demonstrated that salicylic acid-induced immunity could block <italic>R. solani</italic> AG-1 IA infection in both rice and <italic>Brachypodium distachyon</italic>. <italic>R. solani</italic> may undergo biotrophic process using effector proteins to suppress host immunity before necrotrophic stage. To identify pathogen genes expressed at the early infection process, here we developed an inoculation method using <italic>B. distachyon</italic> which enables to sample an increased amount of semi-synchronous infection hyphae. Sixty-one <italic>R. solani secretory effector-like protein</italic> genes (<italic>RsSEPGs</italic>) were identified using in silico approach with the publicly available gene annotation of <italic>R. solani</italic> AG-1 IA genome and our RNA-sequencing results obtained from hyphae grown on agar medium. Expression of <italic>RsSEPGs</italic> was analyzed at 6, 10, 16, 24, and 32 h after inoculation by a quantitative reverse transcription-polymerase chain reaction and 52 genes could be detected at least on a single time point tested. Their expressions showed phase-specific patterns which were classified into 6 clusters. The 23 <italic>RsSEPGs</italic> in the cluster 1–3 and 29 <italic>RsSEPGs</italic> in the cluster 4–6 are expected to be involved in biotrophic and necrotrophic interactions, respectively.

    DOI: 10.1038/s41598-020-71968-x

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  • Endogenous suppressor(s) in Arabidopsis thaliana Reviewed

    Thanh Luan Mai, Tatsuhiro Kawasaki, Aprilia Nur Fitrianti, Le Thi Phuong, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   100 - 106   2020.3

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  • The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes Reviewed

    Le Thi Phuong, Lei Zhao, Aprilia Nur Fitrianti, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   107 - 113   2020.3

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  • Antagonism between SA- and JA-signaling conditioned by saccharin in Arabidopsis thaliana renders resistance to a specific pathogen Reviewed

    Le Thi Phuong, Aprilia Nur Fitrianti, Mai Thanh Luan, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   86 - 99   2020.3

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    DOI: 10.1007/s10327-019-00899-x

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  • Specific growth inhibitors of Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, and Clavibacter michiganensis subsp. michiganensis. Reviewed

    Ombiro GS, Sawai T, Noutoshi Y, Nishina Y, Matsui H, Yamamoto M, Toyoda K, Ichinose Y

    Microbiological research   215   29 - 35   2018.10

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    DOI: 10.1016/j.micres.2018.06.005

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  • Salicylic acid-dependent immunity contributes to resistance against Rhizoctonia solani, a necrotrophic fungal agent of sheath blight, in rice and Brachypodium distachyon. Reviewed

    Kouzai Y, Kimura M, Watanabe M, Kusunoki K, Osaka D, Suzuki T, Matsui H, Yamamoto M, Ichinose Y, Toyoda K, Matsuura T, Mori IC, Hirayama T, Minami E, Noutoshi Y

    New Phytologist   217 ( 2 )   771 - 783   2018.1

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  • Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R-vitis Reviewed

    Kirara Saito, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Akira Kawaguchi, Yoshiteru Noutoshi

    JOURNAL OF GENERAL PLANT PATHOLOGY   84 ( 1 )   58 - 64   2018

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  • AefR transcription factor negatively regulates the virulence of Pseudomonas syringae pv. tomato DC3000 Reviewed

    Ishiga, T, Ishiga, Y, Kiyokawa, T, Maruyama, N, Betsuyaku, S, Ichinose, Y, Nomura, N

    PHYTOPATHOLOGY   107 ( 12::S )   83 - 83   2017.12

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  • Ultrastructural and Cytological Studies on Mycosphaerella pinodes Infection of the Model Legume Medicago truncatula Reviewed

    Tomoko Suzuki, Aya Maeda, Masaya Hirose, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    FRONTIERS IN PLANT SCIENCE   8   2017.6

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    DOI: 10.3389/fpls.2017.01132

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  • Pseudomonas syringae Flood-inoculation Method in Arabidopsis Reviewed

    Yasuhiro Ishiga, Takako Ishiga, Yuki Ichinose, Kirankumar S. Mysore

    BIO-PROTOCOL   7 ( 2 )   2017.1

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  • Pseudomonas syringaeの菌体密度感知機構と多剤排出ポンプの病原力における役割 Invited

    一瀬勇規, 澤田貴博, 髙田基弘, 山本悟, 藤山友里, 中津有紀子, 田阪洋昌, 下村洪祐, 田口富美子, 松井英譲, 山本幹博, 能年義輝, 豊田和弘

    植物細菌病談話会論文集   27   77 - 88   2016.8

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  • Expression profiling of marker genes responsive to the defence-associated phytohormones salicylic acid, jasmonic acid and ethylene in Brachypodium distachyon. Reviewed

    Kouzai Y, Kimura M, Yamanaka Y, Watanabe M, Matsui H, Yamamoto M, Ichinose Y, Toyoda K, Onda Y, Mochida K, Noutoshi Y

    BMC plant biology   16 ( 1 )   59   2016.3

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    DOI: 10.1186/s12870-016-0749-9

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  • The plant cell wall as a site for molecular contacts in fungal pathogenesis Invited Reviewed

    Kazuhiro Toyoda, Sachiyo Yao, Mai Takagi, Maki Uchioki, Momiji Miki, Kaori Tanaka, Tomoko Suzuki, Masashi Amano, Akinori Kiba, Toshiaki Kato, Hirotaka Takahashi, Yasuhiro Ishiga, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   95   44 - 49   2016

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  • Protection induced by volatile limonene against anthracnose disease in Arabidopsis thaliana. Reviewed

    Fujioka, K, Gotoh, H, Noumi, T, Yoshida, A, Noutoshi, Y, Inagaki, Y, Yamamoto, M, Ichinose, Y, Shiraishi, T, Toyoda K

    J Gen Plant Pathol   81 ( 6 )   415 - 419   2015

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  • Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus. Reviewed

    Toyoda K, Kawakami E, Nagai H, Shiobara-Komatsu T, Tanaka K, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol   80 ( 3 )   222 - 229   2014

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    DOI: 10.1007/s10327-014-0510-x

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  • Dynamics of signal perception, signaling and regulation of defenses in plant cell walls.

    TOYODA K., TANAKA K., INAGAKI Y., ICHINOSE Y., SHIRAISHI T.

    Japanese Journal of Phytopathology   80 ( 3 )   146 - 151   2014

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    The plant cell wall is well known to act as a physical barrier against invading pathogens. However, our recent studies have suggested that the plant cell wall can perceive pathogen signals and is involved in signaling and regulation of cell wall-based defenses. Here we will focus on a recently discovered role(s) of the plant cell wall in plant-pathogen interactions to unravel the complicated system underlying plant immunity.

    DOI: 10.3186/jjphytopath.80.146

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  • Defects in D-Rhamnosyl Residue Biosynthetic Genes Affect Lipopolysaccharide Structure, Motility, and Cell-Surface Hydrophobicity in Pseudomonas syringae Pathovar glycinea Race 4 Reviewed

    Kazuhiro Chiku, Kazuhiko Tsunemi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Tadashi Ishii, Fumiko Taguchi, Masako Iwaki, Yuki Ichinose, Hiroshi Ono

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 3 )   505 - 510   2013.3

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  • Suppression of mRNAs for lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR) in pea reduces sensitivity to the phytotoxin coronatine and disease development by Mycosphaerella pinodes. Reviewed

    Toyoda K, Kawanishi Y, Kawamoto Y, Kurihara C, Yamagishi N, Tamura A, Yoshikawa N, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol   79 ( 5 )   321 - 334   2013

  • Isolation and identification of a plant growth-promoting fungus from an agricultural field in Okayama Prefecture.

    Yamagiwa, Y, Toyoda, K, Inagaki, Y, Ichinose, Y, Shiraishi, T

    Sci. Rep. Fac. Agr. Okayama Univ.   102   1 - 6   2013

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  • A volatile substance, beta-caryophyllen, from Talaromyces wortmannii promotes growth and tolerance to disease on several plants.

    Yamagiwa, Y, Toyoda, K, Inagaki, Y, Ichinose, Y, Hyakumachi, M, Shiraishi, T

    Sci. Rep. Fac. Agr. Okayama Univ.   102   7 - 14   2013

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  • The Medicago truncatula-Mycoshaerella pinodes interaction: a new pathosystem for dissecting the fungal suppressor-mediated plant disease susceptibility in plants. Reviewed

    Toyoda K, Ikeda S, Morikawa J, Hirose M, Maeda A, Suzuki T, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol,   79 ( (1) )   1 - 11   2013

  • Plant cell walls as suppliers of potassium and sodium ions for induced resistance of pea (Pisum sativum L.) and cowpea (Vigna ungiculata L.). Reviewed

    Amano M, Toyota K, Kiba A, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol,   79 ( (1) )   12 - 17   2013

  • Infection-inhibition activity of avenacin saponins against the cereal pathogens Blumeria graminis f.sp. hordei, Bipolaris oryzae, and Magnaporthe oryzae. Reviewed

    Inagaki Y, Noutoshi Y, Fujita K, Imaoka A, Arase S, Toyoda K, Shiraishi T, Ichinose Y

    J Gen Plant Pathol,   79 ( (1) )   69 - 73   2013

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    DOI: 10.1007/s10327-012-0412-8

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  • Identification of natural diterpenes that inhibit bacterial wilt disease in Tobacco, Tomato and Arabidopsis Reviewed

    Shigemi Seo, Kenji Gomi, Hisatoshi Kaku, Hiroshi Abe, Hideharu Seto, Shingo Nakatsu, Masahiro Neya, Michie Kobayashi, Kazuhiro Nakaho, Yuki Ichinose, Ichiro Mitsuhara, Yuko Ohashi

    Plant and Cell Physiology   53 ( 8 )   1432 - 1444   2012.8

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  • Characterization of each aefR and mexT mutant in Pseudomonas syringae pv. tabaci 6605. Reviewed

    Kawakita Y, Taguchi F, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y

    Mol. Genet. Genomics   287 ( 6 )   473 - 484   2012

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    DOI: 10.1007/s00438-012-0693-9

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  • H2O2 production by copper amine oxidase, a component of the ecto-apyrase (ATPase)-containing protein complex(es) in the pea cell wall, is regulated by an elicitor and a suppressor from Mycosphaerella pinodes. Reviewed

    Toyoda K, Yasunaga E, Niwa M, Ohwatari Y, Nakashima A, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol,   78 ( (5) )   311 - 315   2012

  • Talaromyces wortmannii FS2 emits beta-caryophyllene, which promotes plant growth and induces resistance. Reviewed

    Yamagiwa Y, Inagaki Y, Ichinose Y, Toyoda K, Hyakumachi M, Shiraishi T

    J. Gen. Plant Pathol.   77 ( (6) )   336 - 341   2011

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    DOI: 10.1007/s10327-011-0340-z

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  • Study of flagella-mediated interactions between plants and Pseudomonas syringae Reviewed

    Yuki Ichinose

    JOURNAL OF GENERAL PLANT PATHOLOGY   75 ( 6 )   452 - 454   2009.12

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    DOI: 10.1007/s10327-009-0192-y

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  • Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin Reviewed

    Tomoyuki Konishi, Fumiko Taguchi, Masako Iwaki, Mayumi Ohnishi-Kameyama, Masanobu Yamamoto, Ikuko Maeda, Yoshihiro Nishida, Yuki Ichinose, Mitsuru Yoshida, Tadashi Ishii

    CARBOHYDRATE RESEARCH   344 ( 16 )   2250 - 2254   2009.11

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    DOI: 10.1016/j.carres.2009.07.004

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  • Study of flagella-mediated interactions between plants and Pseudomonas syringae

    ICHINOSE Y.

    Japanese Journal of Phytopathology   75 ( 3 )   147 - 149   2009

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    DOI: 10.3186/jjphytopath.75.147

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  • 我が国におけるブドウ根頭がんしゅ病菌の遺伝型とその分布

    川口 章, 澤田 宏之, 一瀬 勇規

    土と微生物   62 ( 2 )   143 - 143   2008.6

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    DOI: 10.18946/jssm.62.2_143_3

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  • [MAMP signal transduction].

    Yuki Ichinose

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   52 ( 6 Suppl )   635 - 41   2007.5

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  • Post-translational modification of flagellin determines the specificity of HR induction. Reviewed

    Fumiko Taguchi, Rena Shimizu, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Plant & cell physiology   44 ( 3 )   342 - 9   2003.3

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    DOI: 10.1093/pcp/pcg042

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  • エンドウのエリシター誘導性遺伝子発現におけるAAAGモチーフとPsDof1タンパク質の関与

    關, 光, 丸谷, 瑞理, 稲垣, 善茂, 豊田, 和弘, 白石, 友紀, 一瀬, 勇規

    岡山大學農學部學術報告 = Scientific report of the Faculty of Agriculture, Okayama University   92   21 - 26   2003.2

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    エリシターを処理したエンドウ上胚軸由来のRNAから作成されたcDNAライブラリーからエリシター処理により発現が増高する遺伝子候補のcDNAとしてPsDof1が単離された。大腸菌で生産されたGST-PsDof1融合タンパク質はAAAG配列をコアとするDNAに結合することが明らかにされている。本論文では、GST-PsDof1がエリシター応答性遺伝子の一つ、PsCHS1のプロモーター上のAAAGまたはCTTT配列を有する断片に特異的に結合することを明らかにした。更にAAAG配列のエリシター応答性シスエレメントとしての機能を解析するため、AAAG配列を4回繰り返したユニットをCaMV35Sの最小プロモーターとCATレポーター遺伝子に連結したキメラ遺伝子を構築し、エンドウプロトプラストにエレクトロポレーション法により導入した。CAT活性を指標にプロモーター活性を調べたところ、AAAG配列を有するプロモーターは、エリシター処理により活性化されることが明らかとなった。これらの結果はPsDof1がエリシター応答性防御遺伝子のプロモーター上のAAAG配列に結合し、転写を活性化させる可能性を示唆している。
    identifier:670005
    identifier:ZZ00015342

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  • Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen Reviewed

    Y Ishiga, A Funato, T Tachiki, K Toyoda, T Shiraishi, T Yamada, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   43 ( 10 )   1210 - 1220   2002.10

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    DOI: 10.1093/pcp/pcf144

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  • cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene. Reviewed International journal

    Michiko Sasabe, Kazuhiro Toyoda, Tomonori Shiraishi, Yoshishige Inagaki, Yuki Ichinose

    FEBS letters   518 ( 1-3 )   164 - 8   2002.5

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    DOI: 10.1016/S0014-5793(02)02697-2

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  • 初期・表層シグナル伝達系と防御システム

    白石 友紀, 豊田 和弘, 木場 章範, 一瀬 勇規

    化学と生物   39 ( 10 )   686 - 692   2001.10

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    DOI: 10.1271/kagakutoseibutsu1962.39.686

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  • Suppressors of defense - Supprescins and plant receptor molecules Reviewed

    T Shiraishi, K Toyoda, T Yamada, Y Ichinose, A Kiba, M Sugimoto

    DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS   112 - 121   2001

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  • Bacteriophage P4282, a parasite of Ralstonia solanacearum, encodes a bacteriolytic protein important for lyric infection of its host Reviewed

    H. Ozawa, H. Tanaka, Y. Ichinose, T. Shiraishi, T. Yamada

    Molecular and General Genetics   265 ( 1 )   95 - 101   2001

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  • Regulation of nuclear gene expression in relation to signal molecules Reviewed

    T Yamada, Y Ichinose, T Shiraishi, K Toyoda, Y Imura, H Seki, P Sriprasertsak, A Funado

    DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS   164 - 173   2001

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  • Plant cell wall with the suppressor may play a crucial role in determining specificity Reviewed

    T Shiraishi, A Kiba, A Inata, T Sugiura, K Toyoda, Y Ichinose, T Yamada

    MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES   13   343 - 353   1998

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  • Fungal signals regulate ATPase and polyphosphoinositide metabolism in pea plants Reviewed

    T Shiraishi, T Yamada, Y Ichinose, K Toyoda, A Kiba, T Kato

    MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION   197 - 208   1996

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  • Regulation of genes for phenylpropanoid synthesis in pea by elicitor and suppressor Reviewed

    T Yamada, T Shiraishi, Y Ichinose, H Kato, H Seki, Y Murakami

    MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION   151 - 162   1996

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  • H+-translocating Activity in Proteoliposomes Reconstituted with Pea Plasma Membrane ATPase and Its Inhibition by Fungal Suppressor from Mycosphaerella pinodes.

    AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    Jpn. J. Phytopathol.   61 ( 4 )   369 - 375   1995

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    Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on the H+-translocating activity of ATPase in pea plasma membranes were examined. The plasma membrane ATPase was solubilized with Triton X-100 and partially purified by continuous glycerol density gradient centrifugation. The ATPase fraction was reconstituted into soybean phospholipid (asolectin) liposomes by a Triton-X 100 dilution method. Almost all of resultant proteoliposome vesicles had unimembranes in size from 50 to 200nm. The ATP-driven H+-pumping activity was measured by quinacrine fluorescence quenching in the presence of Mg2+-ATP. The activity was sensitive to orthovanadate, dicyclohexylcar-bodiimide (DCCD), verapamil and neomycin but it was insensitive to azide and nitrate as well as ATP-hydrolyzing activity. Proton gradient was collapsed by NH4Cl or a channel-forming ionophore, gramicidin D. The H+-pumping activity in proteoliposomes was hardly affected by the elicitor. The finding suggests that a putative target molecule of or receptor for the elicitor might not associated with the solubilized ATPase or that the elicitor could not reach the target site. However, the suppressor markedly inhibited both activities in proteoliposomes, showing that the H+-translocating ATPase might be inhibited directly in vitro by the suppressor as reported previously and that the fungal suppressor may disturb the regulation of pH in the host cells. Both activities were increased by the addition of phosphatidylinositolbisphosphate, even if in the presence of the suppressor. However, PIP2 could not completely negate the effect of the suppressor. These results suggest that the H+-pumping activity of plasma membrane ATPase is also regulated by polyphosphoinositide metabolism and that the action sites of the suppressor on the ATPase may be different from those of PIP2.

    DOI: 10.3186/jjphytopath.61.369

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  • Effect of methyl jasmonate on harpin-induced hypersensitive cell heath, generation of hydrogen peroxide and expression of PAL mRNA in tobacco suspension cultured BY-2 cells Reviewed

    S Andi, F Taguchi, K Toyoda, T Shiraishi, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   42 ( 4 )   446 - 449   1992.4

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    DOI: 10.1093/pcp/pce056

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  • 植物病理学 第2版

    眞山, 滋志, 土佐, 幸雄(抗菌性物質分解酵素、細菌の病原性発現機構)

    文永堂出版  2020.3  ( ISBN:9784830041389

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    Total pages:xii, 347p   Language:Japanese

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  • 植物たちの戦争 : 病原体との5億年サバイバルレース

    日本植物病理学会( Role: Joint author ,  3-1, 3-2)

    講談社  2019.3  ( ISBN:9784065152164

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    Total pages:262p   Language:Japanese

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  • 新植物病理学概論

    白石, 友紀, 秋光, 和也, 一瀬, 勇規, 寺岡, 徹, 吉川, 信幸(第4章(細菌病とファイトプラズマ)第12章(植物病理学とバイオテクノロジー))

    養賢堂  2012.3  ( ISBN:9784842504940

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    Total pages:7, 301p, 図版 [1] p   Language:Japanese

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  • Genome-Enabled Analysis of Plant-Pathogen Interactions

    ( Role: Joint author ,  Glycosylation of bacterial flagellins and its role in motility and virulence)

    APS Press (St. Paul, Minnesota, USA)  2011 

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  • Genome-Enabled Analysis of Plant-Pathogen Interactions

    Wolpert, T, Shiraishi, T, Collmer A, Akimitsu, K, Glazebrook, J. ed(Suppression of defense – The role of fungal suppressors in conditioning plant susceptibility.)

    APS Press (St. Paul, Minnesota, USA)  2011 

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  • Genome-Enabled Analysis of Plant-Pathogen Interactions

    Wolpert, T, Shiraishi, T, Collmer A, Akimitsu, K, Glazebrook, J. ed( Role: Joint author ,  Endogenous suppressor in Arabidopsis thaliana.)

    APS Press (St. Paul, Minnesota, USA)  2011 

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  • 植物病理学

    眞山, 滋志, 難波, 成任( Role: Joint author ,  細菌の病原性発現機構(p. 186 - 193))

    文永堂出版  2010.1  ( ISBN:483004117X

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    Total pages:333   Language:Japanese

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  • 遺伝暗号(一瀬勇規)

    基礎演習 産業生物科学(泉本勝利 他編)岡山大学出版会(岡山)p. 83.  2009 

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  • ポリメラーゼ連鎖反応 (PCR) (一瀬勇規)

    基礎演習 産業生物科学(泉本勝利 他編)岡山大学出版会(岡山)p. 84.  2009 

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  • 組換えDNA技術(一瀬勇規)

    基礎演習 産業生物科学(泉本勝利 他編)岡山大学出版会(岡山)p. 88.  2009 

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  • 遺伝子組換え作物(一瀬勇規)

    基礎演習 産業生物科学(泉本勝利 他編)岡山大学出版会(岡山)p. 89.  2009 

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  • 全身獲得抵抗性(一瀬勇規)

    植物ゲノム科学辞典(駒嶺 穆 総編)朝倉書店(東京)p. 196  2009 

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  • 病原性(一瀬勇規)

    植物ゲノム科学辞典(駒嶺 穆 総編)朝倉書店(東京)p. 284.  2009 

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  • 病原力遺伝子(一瀬勇規)

    植物ゲノム科学辞典(駒嶺 穆 総編)朝倉書店(東京)p. 284.  2009 

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  • レース特異的抵抗性(一瀬勇規)

    植物ゲノム科学辞典(駒嶺 穆 総編)朝倉書店(東京)p. 368.  2009 

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  • Role of flagellin glycosylation in bacterial virulence. (Ichinose, Y., Taguchi, F., Takeuchi, K., Suzuki, T., Toyoda, K. and Shiraishi, T.)

    Pseudomonas syringae Pathovars and Related Pathogens - Identification, Epidemiology and Genomics (M. Fatmi et al. eds.) Springer p. 167-174.  2008 

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  • MAMPシグナル伝達機構(一瀬勇規).

    蛋白質 核酸 酵素 増刊号「植物における環境と生物ストレスに対する応答」(島本 功・篠崎一雄・白須 賢・篠崎和子 編) 53 (6) p. 635-641.  2007 

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  • Pseudomonas syringaeによる植物免疫の活性化とその制御機構,微生物の病原性と植物の防御応答(上田一郎編著)p. 132-143.

    北海道大学出版会 札幌  2007 

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  • Role of flagellin glycosylation in bacterial virulence.(Ichinose, Y., Taguchi, F., Takeuchi, K., Suzuki, T., Toyoda, K. and Shiraishi, T.)

    Pseudomonas syringae Pathovars and Related Pathogens - Identification, Epidemiology and Genomics (M. Fatmi et al. eds.) Springer p. 167-174.  2007 

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  • Bacterial flagellins as elicitors of the defense response.

    Genomic and Genetic Analysis of Plant Paratsitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Compatible-specific expression of 12-oxophytodienoic acid 10,11-reductase gene in pea.

    Genomic and Genetic Analysis of Plant Paratsitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Compatible-specific expression of 12-oxophytodienoic acid 10,11-reductase gene in pea.

    Genomic and Genetic Analysis of Plant Paratsitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Flagellin Glycosylation Island in Pseudomonas syringae and Its Role in Host Specificity.

    Genomic and Genetic Analysis of Plant Paratsitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Defense signaling snd the plant cell wall – A new signaling pathway dependent upon inorganic phosphate.

    Genomic and Genetic Analysis of Plant Parasitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Flagellin Glycosylation Island in Pseudomonas syringae and Its Role in Host Specificity.

    Genomic and Genetic Analysis of Plant Paratsitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Defense signaling snd the plant cell wall – A new signaling pathway dependent upon inorganic phosphate.

    Genomic and Genetic Analysis of Plant Parasitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • :植物病原菌のシグナル分子により発現が変動する遺伝子の制御機構と機能

    植物病の探究 (高松 進ら編)  2004 

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  • Bacterial flagellins as elicitors of the defense response.

    Genomic and Genetic Analysis of Plant Paratsitism and Defense (Tsuyumu et al. eds.) APS Press (St. Paul Minnesota, USA)  2004 

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  • Suppressors of defense-Supprescins and Plant receptor molecules.

    Delivery of Pathogen Signals to Plants (N. T. Keen ed.) APS Press (St. Paul Minnesota, USA)  2001 

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  • Regulation of nuclear gene expression in relation to signal molecules.

    Delivery of Pathogen Signals to Plants (N. T. Keen ed.) APS Press (St. Paul Minnesota, USA)  2001 

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  • Suppressors of defense-Supprescins and Plant receptor molecules.

    Delivery of Pathogen Signals to Plants (N. T. Keen ed.) APS Press (St. Paul Minnesota, USA)  2001 

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  • Regulation of nuclear gene expression in relation to signal molecules.

    Delivery of Pathogen Signals to Plants (N. T. Keen ed.) APS Press (St. Paul Minnesota, USA)  2001 

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  • 病原体に対する応答

    朝倉植物生理学講座第5巻環境応答  2001 

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  • Suppressors of defense-Supprescins and Plant receptor molecules.(共著)

    Delivery of Pathogen Signals to Plants(N. T. Keen Ed. )APS Press, St. Paul Minnesota, USA  2000 

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  • Regulation of nuclear gene expression in relation to signal molecules.(共著)

    Delivery of Pathogen Signals to Plants(N. T. Keen Ed. )APS Press, St Paul Minnesota, USA  2000 

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  • 宿主特異性決定における植物細胞壁の役割(共著)

    東北地方における植物病理学のフロントラン日本植物病理学会東北部会創立35周年記念誌刊行会  2000 

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  • Suppressors of defense-Supprescins and Plant receptor molecules.(共著)

    Delivery of Pathogen Signals to Plants(N. T. Keen Ed. )APS Press, St. Paul Minnesota, USA  2000 

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  • Regulation of nuclear gene expression in relation to signal molecules.(共著)

    Delivery of Pathogen Signals to Plants(N. T. Keen Ed. )APS Press, St Paul Minnesota, USA  2000 

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  • Suppressor as a factor determining plant-pathogen Specificity.(共著)

    Plant-Microbe Interactions Vol. 4, (Stacey, G. and Keen, N. T. eds. )APS Press, St, Paul Minnesota, USA  1999 

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  • Suppressor as a factor determining plant-pathogen Specificity.(共著)

    Plant-Microbe Interactions Vol. 4, (Stacey, G. and Keen, N. T. eds. )APS Press, St, Paul Minnesota, USA  1999 

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  • The role of suppressors in determining host-parasite specificities in plant cells(共著)

    International Review of Cytology, Academic Press  1997 

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  • The role of suppressors in determining host-parasite specificities in plant cells(共著)

    International Review of Cytology, Academic Press  1997 

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  • 目で見る植物の耐病性(共著)

    植物細胞工学シリーズ8「分子レベルからみた植物の耐病性」  1997 

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  • 宿主特異性と防御遺伝子の発現調節(共著)

    植物細胞工学シリーズ8「分子レベルからみた植物の耐病性」  1997 

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  • Regulation of the gene involved in pheylpropanoid pathway in pea by eliutor and suppressor(共著)

    Molecular Aspects of Phathogenisity and Host Resistance (Requirement of Signal Transduction) APS Press  1996 

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  • 植物の病原体認識とシグナル伝達(共著)

    植物のシグナルトランスダクション,東京化学同人  1996 

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  • Fungal signals regulate ATPase and polyphosphoinositide metabolism in pea plant(共著)

    Molecular Aspects of Phathogenisity and Host Resistance (Requirement of Signal Transduction) APS Press  1996 

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  • Regulation of the gene involved in pheylpropanoid pathway in pea by eliutor and suppressor(共著)

    Molecular Aspects of Phathogenisity and Host Resistance (Requirement of Signal Transduction) APS Press  1996 

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  • Fungal signals regulate ATPase and polyphosphoinositide metabolism in pea plant(共著)

    Molecular Aspects of Phathogenisity and Host Resistance (Requirement of Signal Transduction) APS Press  1996 

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  • Regulation of ATPase and signal transduction for pea defense responses by the suppressor and elicitor from ┣DBMycosphaerella pinodes(/)-┫DB. (共著)

    Host-Specific Toxin : Biosynthesis, Receptor and Molecular Biology (eds. K. Kohmoto and O. C. Yoder). Tottori University, Tottori.  1993 

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  • 植物病原性微生物研究法 共著

    ソフトサイエンス社  1993 

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  • Regulation of ATPase and signal transduction for pea defense responses by the suppressor and elicitor from ┣DBMycosphaerella pinodes(/)-┫DB. (共著)

    Host-Specific Toxin : Biosynthesis, Receptor and Molecular Biology (eds. K. Kohmoto and O. C. Yoder). Tottori University, Tottori.  1993 

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MISC

  • Infectivity of Rhizoctonia solani isolates on Brachypodium distachyon and barley, and chemical-induced resistance

    MAHADEVAN Neranjana, FERNANDA Rozi, MATSUI Hidenori, ICHINOSE Yuki, TOYODA Kazuhiro, HISANO Hiroshi, KOUZAI Yusuke, OKAMOTO Masanori, OKAMOTO Masanori, NOUTOSHI Yoshiteru

    日本植物病理学会大会プログラム・講演要旨予稿集   2024   2024

  • N-hydroxypipecolic acid confers resistance to Rhizoctonia Solon in barley and Brachypodium distachyon

    MAHADEVAN Neranjana, KOHNO Natsuka, NAGAO Reiko, NYEIN Khin Thida, MATSUI Hidenori, YAMAMOTO Mikihiro, ICHINOSE Yuki, TOYODA Kazuhiro, KOUZAI Yusuke, HISANO Hiroshi, OKAMOTO Masanori, OKAMOTO Masanori, NOUTOSHI Yoshiteru

    日本植物病理学会大会プログラム・講演要旨予稿集   2023   2023

  • Colonization ability of Rhizobium vitis VAR03-1, a biocontrol agent for grapevine crown gall disease, in Arabidopsis thaliana.

    BAO J., 松井英讓, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   88 ( 1 )   2022

  • A study of the host factors targeted by Pseudomonas syringae pv. tabaci type III effector J.

    樫原沙知, 西村隆史, 中神弘史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   88 ( 1 )   2022

  • Characterization of rhizoviticin, a phage tail-like particle, as a determinant of biocontrol activity of Rhizobium vitis VAR03-1 on grapevine crown gall disease.

    土田菜月, 石井智也, 渡邉恵, 齊藤晶, BAO J., 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   88 ( 1 )   2022

  • Functional analysis of the plant immune signaling pathway mediated by MAMP-responsive Raf-like protein kinase 1 (MRPK1) in Arabidopsis.

    藤山祐香, 松井英譲, 松井英譲, HYON G.-S., 野村有子, 能年義輝, 豊田和弘, 一瀬勇規, 中神弘史, 中神弘史

    日本植物病理学会報   88 ( 1 )   2022

  • Colonization mechanism of Rhizobium vitis VAR03-1, a biocontrol agent for grapevine crown gall disease, on Arabidopsis thaliana

    BAO Jiyuan, HEMELDA Niarsi Merry, 土田菜月, 渡邉恵, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 豊田和弘, 豊田和弘, 一瀬勇規, 一瀬勇規, 川口章, 能年義輝, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • CEP peptides, a family of conserved, secreted peptides that negatively modulate Arabidopsis immunity: IV. CEP peptide attenuates SA-mediated immunity

    長谷川晴香, 伊藤千晶, FITRIANTI Aprilia Nur, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 能年義輝, 能年義輝, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   31st   2022

  • CEP peptides, a family of conserved, secreted peptides that negatively modulate Arabidopsis immunity: III. Potential role during PTI and ETI

    伊藤千晶, 長谷川晴香, FITRIANTI Aprilia Nur, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 能年義輝, 能年義輝, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   31st   2022

  • Upregulation of CEP genes in Arabidopsis leaves challenged with avirulent and non-adapted bacteria

    APRILIA Nur Fitrianti, 伊藤千晶, 長谷川晴香, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Towards a comprehensive identification of host factors targeted by Type III effectors of the tobacco wildfire

    黒江香那, 樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Functional analysis of type III effector J protein of Psedomonas syringae pv. tabaci 6605

    樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Identification of effectors contributing to the development of disease symptom in Tobacco wildfire pathogens.

    西村隆史, 樫原沙知, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   87 ( 1 )   2021

  • Involvement of cell wall peroxidase and NADPH oxidase in MAMP-induced oxidative burst in Arabidopsis.

    木元菜々子, 高須瑞穂, APRILIA Nur Fitrianti, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   87 ( 1 )   2021

  • A possible role of an NADPH oxidase RBOHD in regulating intra/extracellular redox in Arabidopsis thaliana.

    高須瑞穂, 木元菜々子, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   87 ( 1 )   2021

  • Recognition of pathogenic fungi by unknown receptor(s) in Arabidopsis thaliana.

    矢野裕奈, APRILIA Nur Fitrianti, 木元菜々子, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   87 ( 1 )   2021

  • Study on the Mode of Action of Metominostrobin as a Plant Activator 5. Effect on MAP Kinases During PTI in Arabidopsis

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Search for virulence factors in Pseudomonas syringae pv. tabaci using comparative genomic analysis

    吉岡桂, 西村隆史, 浅井秀太, 増田幸子, 白須賢, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Tobacco wildfire-resistant cultivars recognize the Pta TTSS effector and induce HR.

    樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   87 ( 1 )   2021

  • Study on the mode of action of metominostrobin as a plant activator 3. Metominostrobin causes sustained activation of MAP kinases in Arabidopsis immunity.

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本農薬学会大会講演要旨集   46th (CD-ROM)   2021

  • Study on the Mode of Action of Metominostrobin as a Plant Activator 6. Effect on Expression of PTI-related Genes in Arabidopsis

    小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • The involvement of chemoreceptor gene mcp on virulence in Pseudomonas syringae pv. tabaci 6605

    寺元茜, 金重慎, TUMEWU S.A., 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Phosphorylation of MARK2 is involved in callose accumulation upon flg22 treatment.

    松井英譲, 松井英譲, 澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 一瀬勇規, 中神弘史, 中神弘史

    日本植物病理学会報   87 ( 1 )   2021

  • Study on the mode of action of metominostrobin as a plant activator 4. Metominostrobin enhances expression of PTI-related genes in Arabidopsis.

    小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本農薬学会大会講演要旨集   46th (CD-ROM)   2021

  • Prerequisites for the oxidative burst in Arabidopsis: A role of NADPH oxidase, RBOHD in regulating intra/extracellular redox

    高須瑞穂, 木元菜々子, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Wildfire disease resistant tobacco recognizes type III secretion effector of Pseudomonas syringae pv. tabaci

    樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • mark2 mutant reduces Jasmonate-related gene expression during Pto infection

    澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 一瀬勇規, 松井英譲, 松井英譲, 中神弘史, 中神弘史

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Immunity response of Brachypodium distachyon induced by microbe-associated molecular patterns.

    小笠原翼, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 持田恵一, 能年義輝

    日本植物病理学会報   86 ( 1 )   2020

  • Search of major virulence factors of Pseudomonas syringae pv. tabaci.

    西村隆史, 樫原沙知, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   86 ( 1 )   2020

  • Arabidopsis class III peroxidase PRX34 plays a role in the MAMP-elicited oxidative burst.

    高須瑞穂, 木元菜々子, LEI Zhao, PHUONG Le Thi, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   86 ( 1 )   2020

  • Pseudomonas syringae pv. tabaci effectors that contribute to the development of disease symptom

    西村隆史, 樫原沙知, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • c-di-GMP-related gene 1 (CDG1) contributes to the regulation of virulence in Pta 6605

    澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Contribution of cell wall peroxidase and NADPH oxidase to the MAMP-induced oxidative burst in Arabidopsis

    木元菜々子, 高須瑞穂, APRILIA NUR F., ZHAO L., LE THI P., 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • A tailocin responsible for antagonistic activity of Rhizobium vitis VAR03-1 against grapevine crown gall disease

    石井智也, 齋藤晶, 渡邉恵, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, BAO J., 松井英譲, 山本幹博, 豊田和弘, 一瀬勇規, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Recognition of fungal pathogen in Arabidopsis, that does not require a known receptor

    矢野裕奈, APRILIA NUR F., 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Studies on the mode of action of metominostrobin II. Priming against powdery mildew resistance in wheat.

    佐藤穂高, PHUONG Le Thi, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   86 ( 1 )   2020

  • Kinetics study of Arabidopsis salicylic acid glucosyltransferase inhibitors as potential plant activators.

    篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

    日本植物病理学会報   86 ( 1 )   2020

  • Characterization of expression profiles of effector genes of Rhizoctonia solani on Brachypodium distachyon

    ABDELSALAM S. S. H., ISOYA S., WATANABE M., KOUZAI Y., MATSUI H., YAMAMOTO M., ICHINOSE Y., TOYODA K., MOCHIDA K., TSUGE S., NOUTOSHI Y.

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • 抵抗性誘導剤のシーズとして単離したサリチル酸配糖化酵素阻害剤の反応速度論解析

    篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

    日本植物病理学会報   85 ( 1 )   2019

  • 植物免疫活性剤インプリマチンDがpattern-triggered immunityに与える影響

    山枡一秀, 平田奈美, 福井絢子, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • MAMP応答性タンパク質MARK2の病原菌感染時の機能解析

    澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 一瀬勇規, 一瀬勇規, 松井英譲, 松井英譲, 松井英譲, 中神弘史, 中神弘史

    日本植物病理学会報   85 ( 1 )   2019

  • 微生物分子パターンに対するミナトカモジグサの活性酸素種生成特性の解析

    小笠原翼, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 香西雄介, 能年義輝

    日本植物病理学会報   85 ( 1 )   2019

  • Pseudomonas syringae pv.tabaciのRNAタイプ多剤排出トランスポーターの病原力における機能解析

    西村隆史, 一瀬勇規, 原田みのり, 松井英譲, 山本幹博, 能年義輝, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • エンドウの細胞壁画分に見出されたキトサンと結合するタンパク質複合体

    松尾実佳, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

    松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   85 ( 1 )   2019

  • 非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制機構の解析

    石井智也, 渡邉恵, 齊藤晶, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   85 ( 1 )   2019

  • タバコ野火病菌のCDG1はアシルホモセリンラクトンの蓄積を正に制御する

    澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

    日本植物病理学会報   85 ( 1 )   2019

  • メトミノストロビンの作用機構に関する研究

    佐藤穂高, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   85 ( 1 )   2019

  • 細胞外オキシダティブバースト反応の分子機構:ペルオキシダーゼのスルフェン酸化とその役割

    高須瑞穂, 吉岡美樹, 吉岡博文, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   85 ( 1 )   2019

  • Pseudomonas syringaeの菌体密度感知機構の解析(4)菌体密度に応じた遺伝子発現制御

    中津有紀子, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • Pseudomonas syringaeの菌体密度感知機構の解析(3)PsyRとアシルホモセリンラクトンによる遺伝子発現制御機構

    田阪洋昌, 山本悟, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • バイオコントロール細菌Rhizobium vitis VAR03-1株のブドウ根頭がんしゅ病抑制機構の解析

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

    松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   28th   2018

  • 細菌シグナル伝達因子CDG proteinの機能解析

    澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • MAMP応答性リン酸化タンパク質MARK2の機能解析

    澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G.-S., 野村有子, 一瀬勇規, 松井英譲, 松井英譲, 中神弘史, 中神弘史

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • Pseudomonas syringaeの菌体密度感知機構の解析(2)

    一瀬勇規, 松井英譲, 石賀康博, 山本幹博, 能年義輝, 豊田和弘

    日本植物病理学会報   84 ( 1 )   2018

  • 非病原性Rhizobium vitis VAR03-1株の培養上清がブドウ根頭がんしゅ病菌に及ぼす影響

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   84 ( 1 )   2018

  • ササゲにおける細胞外オキシダティブバースト反応の分子機構

    川端真矢, 佐藤穂高, 高須瑞穂, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   84 ( 1 )   2018

  • Pseudomonas syringaeの菌体密度感知機構の解析(1)

    山本悟, 藤山友里, 高田基弘, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   83 ( 1 )   2017

  • エンドウならびにシロイヌナズナにおけるPAMP誘導性細胞外オキシダティブバースト反応の分子機構

    片岡千香子, 三木紅葉, 山崎史織, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017

  • 非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017

  • エンドウから分離した細胞壁タンパク質のPAMP応答

    三木紅葉, 山崎史織, 片岡千香子, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • 非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   83 ( 1 )   2017

  • エクト型ATPase(PsAPY1)はエンドウ葉におけるPAMP誘導性オキシダティブバースト反応を正に調節し,不適応型の病原菌に対する非宿主抵抗性に関与する

    山崎史織, 三木紅葉, 矢尾幸世, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • ミナトカモジグサにおける植物免疫ホルモン応答性マーカー遺伝子の同定と発現プロファイルの解析

    香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 恩田義彦, 持田恵一, 能年義輝

    日本植物病理学会報   83 ( 1 )   2017

  • シロイヌナズナにおける細胞外オキシダティブバースト反応の分子機構

    松尾実佳, 片岡千香子, 山崎史織, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • シロイヌナズナにおけるPAMP誘導性オキシダティブバースト反応におけるアポプラストの役割について

    片岡千香子, 山崎史織, 松尾実佳, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • Pseudomonas syringae pv.tabaci6605におけるAHL合成制御機構並びにMexEFOprN多剤排出ポンプの病原力における役割の解析

    澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   83 ( 1 )   2017

  • エクト型ATPase(PsAPY1)はエンドウ葉におけるリポ多糖(LPS)誘導性細胞外オキシダティブバースト反応を正に調節する

    三木紅葉, 山崎史織, 矢尾幸世, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • 非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の発病発現抑制機構の解析

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • ナス科植物青枯病菌の病原力や増殖を制御する化合物の探索

    澤井拓, 一瀬勇規, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 向原隆文, 長田裕之

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • ミナトカモジグサ-紋枯病菌感染系の確立と植物ホルモンによる抵抗性機構の解析

    香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 恩田義彦, 持田恵一, 山本幹博, 松井英譲, 一瀬勇規, 豊田和弘, 能年義輝

    日本植物病理学会報   82 ( 1 )   2016

  • Pseudomonas syringae pv.syringae B728aの病原力制御因子Vfrの標的遺伝子のクロマチン免疫沈降法による探索(2)

    小倉敬右, 田口富美子, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • エクト型ATPase(PsAPY1)の発現を抑制したエンドウ葉におけるジャスモン酸応答性遺伝子の発現

    矢尾幸世, 田中佳織, 奥田竜太, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

    日本植物病理学会報   82 ( 1 )   2016

  • Pseudomonas syringae pv.tabaciにおけるAHL合成制御機構の解析

    澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   82 ( 1 )   2016

  • サリチル酸配糖化酵素を用いた抵抗性誘導剤の標的ベース探索

    谷川友里佳, 渡邉恵, 熊谷和夫, 香西雄介, 山中由利恵, 木村麻美子, 稲垣善茂, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

    日本植物病理学会報   81 ( 1 )   2015

  • 植物免疫プライミング剤インプリマチンDの作用解析

    福井絢子, 香西雄介, 稲垣善茂, 山本幹博, 一瀬勇規, 豊田和弘, 白須賢, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2015   2015

  • Pathogenicity and virulence factors of Pseudomonas syringae

    Yuki Ichinose, Fumiko Taguchi, Takafumi Mukaihara

    Journal of General Plant Pathology   79 ( 5 )   285 - 296   2013.9

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    DOI: 10.1007/s10327-013-0452-8

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  • A volatile substance from Talaromyces sp promotes the plant growth and blocks the disease development on several plants

    T. Shiraishi, Y. Yamagiwa, P. T. Le, K. Maeda, Y. Inagaki, Y. Ichinose, M. Hyakumachi, K. Toyoda

    PHYTOPATHOLOGY   101 ( 6 )   S165 - S166   2011.6

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  • 立枯病・青枯病抵抗性誘導物質の単離同定と作用機構の解析

    瀬尾茂美, 五味剣二, 加来久敏, 中保一浩, 小林光智衣, 瀬戸秀春, 安部洋, 一瀬勇規, 光原一朗, 大橋祐子

    日本植物生理学会年会要旨集   52nd   222   2011.3

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  • The Siderophore Pyoverdine of Pseudomonas syringae pv. tabaci 6605 Is an Intrinsic Virulence Factor in Host Tobacco Infection Reviewed

    Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of Bacteriology   192 ( 1 )   117 - 126   2010.1

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    DOI: 10.1128/jb.00689-09

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  • Bacterial DNA activates immunity in Arabidopsis thaliana

    Suguru Yakushiji, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    JOURNAL OF GENERAL PLANT PATHOLOGY   75 ( 3 )   227 - 234   2009.6

  • Bacterial DNA activates immunity in Arabidopsis thaliana

    Suguru Yakushiji, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   75 ( 3 )   227 - 234   2009.6

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  • Enhanced Defense Responses in Arabidopsis Induced by the Cell Wall Protein Fractions from Pythium oligandrum Require SGT1, RAR1, NPR1 and JAR1

    Yoko Kawamura, Shigehito Takenaka, Shu Hase, Mayumi Kubota, Yuki Ichinose, Yoshinori Kanayama, Kazuhiro Nakaho, Daniel F. Klessig, Hideki Takahashi

    Plant and Cell Physiology   50 ( 5 )   924 - 934   2009.5

  • Enhanced Defense Responses in Arabidopsis Induced by the Cell Wall Protein Fractions from Pythium oligandrum Require SGT1, RAR1, NPR1 and JAR1

    Yoko Kawamura, Shigehito Takenaka, Shu Hase, Mayumi Kubota, Yuki Ichinose, Yoshinori Kanayama, Kazuhiro Nakaho, Daniel F. Klessig, Hideki Takahashi

    PLANT AND CELL PHYSIOLOGY   50 ( 5 )   924 - 934   2009.5

  • High Level Expression of a Virus Resistance Gene, RCY1, Confers Extreme Resistance to Cucumber mosaic virus in Arabidopsis thaliana

    Ken-Taro Sekine, Sayaka Kawakami, Shu Hase, Mayumi Kubota, Yuki Ichinose, Jyoti Shah, Hong-Gu Kang, Daniel F. Klessig, Hideki Takahashi

    MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 11 )   1398 - 1407   2008.11

  • High Level Expression of a Virus Resistance Gene, RCY1, Confers Extreme Resistance to Cucumber mosaic virus in Arabidopsis thaliana

    Ken-Taro Sekine, Sayaka Kawakami, Shu Hase, Mayumi Kubota, Yuki Ichinose, Jyoti Shah, Hong-Gu Kang, Daniel F. Klessig, Hideki Takahashi

    MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 11 )   1398 - 1407   2008.11

  • Biological Control of Crown Gall of Grapevine, Rose, and Tomato by Nonpathogenic Agrobacterium vitis Strain VAR03-1

    A. Kawaguchi, K. Inoue, Y. Ichinose

    PHYTOPATHOLOGY   98 ( 11 )   1218 - 1225   2008.11

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    DOI: 10.1094/PHYTO-98-11-1218

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  • Biological Control of Crown Gall of Grapevine, Rose, and Tomato by Nonpathogenic Agrobacterium vitis Strain VAR03-1

    A. Kawaguchi, K. Inoue, Y. Ichinose

    PHYTOPATHOLOGY   98 ( 11 )   1218 - 1225   2008.11

  • Amino Acid Sequence of Bacterial Microbe-Associated Molecular Pattern flg22 Is Required for Virulence International journal

    Kana Naito, Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 9 )   1165 - 1174   2008.9

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    DOI: 10.1094/MPMI-21-9-1165

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  • Amino Acid Sequence of Bacterial Microbe-Associated Molecular Pattern flg22 Is Required for Virulence

    Kana Naito, Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 9 )   1165 - 1174   2008.9

  • (129) Molecular Analysis for Oxidosqualene Cyclase in Saponin Biosynthesis Pathway of Rice(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Inagaki Y., Imaoka A., Fujita K., Arase S., Toyoda K., Shiraishi T., Ichinose Y., Osbourn Anne

    Annals of the Phytopathological Society of Japan   74 ( 3 )   201 - 201   2008.8

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  • (308) Structural Analysis of Flagellin Glycan and Functional Analysis of Genes Involved in Flagellin Glycosylation in Pseudomonas syringae(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Tsunemi K, Taguchi F, Takeuchi K, Ishii T, Yoshida M, Ono Y, Kameyama M, Ichinose Y

    Annals of the Phytopathological Society of Japan   74 ( 3 )   249 - 249   2008.8

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  • (127) Functional Analysis of Cell Wall-associated apyrase MtAPY1; 1 in Medicago truncatula(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Shiobara T., Fujita K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   200 - 200   2008.8

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  • Phylogenetic and serological analyses reveal genetic diversity of Agrobacterium vitis strains in Japan

    A. Kawaguchi, H. Sawada, Y. Ichinose

    PLANT PATHOLOGY   57 ( 4 )   747 - 753   2008.8

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    DOI: 10.1111/j.1365-3059.2008.01829.x

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  • Phylogenetic and serological analyses reveal genetic diversity of Agrobacterium vitis strains in Japan

    A. Kawaguchi, H. Sawada, Y. Ichinose

    PLANT PATHOLOGY   57 ( 4 )   747 - 753   2008.8

  • Polyphosphate kinase is essential for swarming motility, tolerance to environmental stresses, and virulence in Pseudomonas syringae pv. tabaci 6605

    Md. Mijan Hossain, Chiharu Tani, Tomoko Suzuki, Fumiko Taguchi, Tatsuhiro Ezawa, Yuki Ichinose

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   72 ( 4-6 )   122 - 127   2008.7

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    DOI: 10.1016/j.pmpp.2008.04.007

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  • Polyphosphate kinase is essential for swarming motility, tolerance to environmental stresses, and virulence in Pseudomonas syringae pv. tabaci 6605

    Md. Mijan Hossain, Chiharu Tani, Tomoko Suzuki, Fumiko Taguchi, Tatsuhiro Ezawa, Yuki Ichinose

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   72 ( 4-6 )   122 - 127   2008.7

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  • Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction

    Mizuri Marutani, Fumiko Taguchi, Yujiro Ogawa, Md. Mijan Hossain, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   279 ( 4 )   313 - 322   2008.4

  • Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction

    Mizuri Marutani, Fumiko Taguchi, Yujiro Ogawa, Md. Mijan Hossain, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   279 ( 4 )   313 - 322   2008.4

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  • Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana

    Kuniaki Higashi, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   279 ( 3 )   303 - 312   2008.3

  • Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana International journal

    Kuniaki Higashi, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   279 ( 3 )   303 - 312   2008.3

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    DOI: 10.1007/s00438-007-0315-0

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  • (72)Role of Flagellin Glycosylation on Flagellar Formation and Motility in Pseudomonas syringae pv. tabaci(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Taguchi F., Shibata S., Suzuki T., Ogawa Y., Aizawa S., Ichinose Y.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   75 - 75   2008.2

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  • Effects of glycosylation on swimming ability and flagellar polymorphic transformation in Pseudomonas syringae pv. tabaci 6605 International journal

    Fumiko Taguchi, Satoshi Shibata, Tomoko Suzuki, Yujiro Ogawa, Shin-Ichi Aizawa, Kasumi Takeuchi, Yuki Ichinose

    JOURNAL OF BACTERIOLOGY   190 ( 2 )   764 - 768   2008.1

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    DOI: 10.1128/JB.01282-07

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  • Effects of glycosylation on swimming ability and flagellar polymorphic transformation in Pseudomonas syringae pv. tabaci 6605

    Fumiko Taguchi, Satoshi Shibata, Tomoko Suzuki, Yujiro Ogawa, Shin-Ichi Aizawa, Kasumi Takeuchi, Yuki Ichinose

    JOURNAL OF BACTERIOLOGY   190 ( 2 )   764 - 768   2008.1

  • Molecular analysis for genome structure and gene expression of Oxidosqualene Cyclase genes in rice seedling

    Yoshishige Inagaki, Yukako Mutsukado, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Anne Osbourn

    GENES & GENETIC SYSTEMS   82 ( 6 )   521 - 521   2007.12

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  • Flagellin Glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose

    Kasumi Takeuchi, Hiroshi Ono, Mitsuru Yoshida, Tadashi Ishii, Etsuko Katoh, Fumiko Taguchi, Ryuji Miki, Katsuyoshi Murata, Hanae Kaku, Yuki Ichinose

    JOURNAL OF BACTERIOLOGY   189 ( 19 )   6945 - 6956   2007.10

  • N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana

    Kana Naito, Yasuhiro Ishiga, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   73 ( 4 )   281 - 285   2007.8

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  • Suppression of Cdc27B expression induces plant defence responses

    Chikako Kudo, Tomoko Suzuki, Sumie Fukuoka, Shuta Asai, Hiroko Suenaga, Michiko Sasabe, Yoshitaka Takano, Tetsuro Okuno, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Yoshi-Shige Inagaki

    MOLECULAR PLANT PATHOLOGY   8 ( 4 )   365 - 373   2007.7

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  • Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes.

    Suzuki, T, Maeda, A, Hirose, M, Ichinose, Y, Toyoda, K, Shiraishi, T

    J. Electr. Microsc. Technol. Med. Biol.   20 ( 2 )   167 - 168   2007

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  • Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

    Michiko Sasabe, Kana Naito, Hiroko Suenaga, Takako Ikeda, Kazuhiro Toyodak, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

    DNA SEQUENCE   18 ( 2 )   152 - 159   2007

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  • Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes.

    Suzuki, T, Maeda, A, Hirose, M, Ichinose, Y, Toyoda, K, Shiraishi, T

    J. Electr. Microsc. Technol. Med. Biol.   20 ( 2 )   167 - 168   2007

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  • A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis International journal

    Fumiko Taguchi, Yujiro Ogawa, Kasumi Takeuchi, Tomoko Suzuki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    JOURNAL OF BACTERIOLOGY   188 ( 24 )   8376 - 8384   2006.12

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    DOI: 10.1128/JB.00763-06

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  • A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis

    Fumiko Taguchi, Yujiro Ogawa, Kasumi Takeuchi, Tomoko Suzuki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    JOURNAL OF BACTERIOLOGY   188 ( 24 )   8376 - 8384   2006.12

  • ダイズ斑点細菌病菌のフラジェリン糖鎖解析

    竹内香純, 田口富美子, 加藤悦子, 三木隆二, 村田勝義, 賀来華江, 一瀬勇規

    日本植物病理学会報   72 ( 4 )   310   2006.11

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  • Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen

    Tomonari Kasai, Tomoko Suzuki, Kozue Ono, Ken'Ichi Ogawa, Yoshishige Inagaki, Yuki Ichinose, Kazuhiro Toyoda, Tomonori Shiraishi

    Journal of General Plant Pathology   72 ( 5 )   265 - 272   2006.10

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  • A binding protein for fungal signal molecules in the cell wall of Pisum sativum

    Akinori Kiba, Takako Ohgawara, Kazuhiro Toyoda, Miho Inoue-Ozaki, Tadahiro Takeda, Uppalapati Srinivasa Rao, Toshiaki Kato, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   72 ( 4 )   228 - 237   2006.8

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  • A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms

    Akinori Kiba, Kazuhiro Toyoda, Kazuaki Yoshioka, Kami Tsujimura, Hirotaka Takahashi, Yuki Ichinose, Tadahiro Takeda, Toshiaki Kato, Tomonori Shiraishi

    Journal of General Plant Pathology   72 ( 4 )   238 - 246   2006.8

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  • Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms

    Hirotaka Takahashi, Kazuhiro Toyoda, Yuzo Hirakawa, Kunihiko Morishita, Toshiaki Kato, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   72 ( 3 )   143 - 151   2006.6

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  • Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci

    F Taguchi, K Takeuchi, E Katoh, K Murata, T Suzuki, M Marutani, T Kawasaki, M Eguchi, S Katoh, H Kaku, C Yasuda, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    CELLULAR MICROBIOLOGY   8 ( 6 )   923 - 938   2006.6

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  • Induction of defense responses in pea tissues by inorganic phosphate

    Tomoharu Kawahara, Hiroko Namba, Kazuhiro Toyoda, Tomonari Kasai, Megumi Sugimoto, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   72 ( 3 )   129 - 136   2006.6

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  • Functional analysis of Gac two-component system in P syringae pv. tabaci

    M. Marutani, Y. Ogawa, F. Taguchi, Y. Inagaki, K. Toyoda, T. Shiraishi, Y. Ichinose

    PHYTOPATHOLOGY   96 ( 6 )   S74 - S74   2006.6

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  • 植物の非宿主抵抗性

    一瀬勇規

    化学と生物   44 ( 8 )   556 - 562   2006

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    DOI: 10.1271/kagakutoseibutsu1962.44.556

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  • AtOPR3高発現シロイヌナズナを用いた植物病原菌相互作用におけるOPR subgroup 2の機能解析

    松井英譲, 豊田和弘, 稲垣善茂, 白石友紀, 一瀬勇規

    日本植物病理学会報   72 ( 4 )   2006

  • (18) Rer1 Gene Homologues Expressed in Medicago truncatula and Barley Challenged with Virulent Pathogens(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Yoshihiro M., Hirose M., Fujita K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   190 - 190   2005.8

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  • Flagellin from Pseudomonas syringae pv. tabaci induced hrp-independent HR in tomato

    Mizuri Marutani, Fumiko Taguchi, Rena Shimizu, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   71 ( 4 )   289 - 295   2005.8

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  • Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

    Yasuhiro Ishiga, Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   71 ( 4 )   302 - 307   2005.8

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  • Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

    Yasuhiro Ishiga, Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   71 ( 4 )   302 - 307   2005.8

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  • Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells

    Dirk Schenke, Kana Naito, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   71 ( 4 )   273 - 279   2005.8

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  • Identification of genes expressed during spore germination of Mycosphaerella pinodes

    Hiroyuki Takahara, Kazuhiro Toyoda, Gento Tsuji, Yasuyuki Kubo, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   71 ( 3 )   190 - 195   2005.6

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  • 植物病原細菌Pseudomonas syringaeのべん毛タンパク質フラジェリンの糖鎖修飾と植物相互作用

    竹内香純, 田口富美子, 三木隆二, 安田千裕, 村田勝義, 加藤悦子, 加藤静恵, 賀来華江, 稲垣善茂, 豊田和弘, 白石友紀, 一瀬勇規

    植物微生物研究会研究交流会講演要旨集   14th   (JA)120,(EN)121   2005.3

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  • Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens

    Hideki Takahashi, Ayano Shimizu, Tsutomu Arie, Syofi Rosmalawati, Sumire Fukushima, Mari Kikuchi, Yasufumi Hikichi, Ayami Kanda, Akiko Takahashi, Akinori Kiba, Kohei Ohnishi, Yuki Ichinose, Fumiko Taguchi, Chihiro Yasuda, Motoichiro Kodama, Mayumi Egusa, Chikara Masuta, Hiroyuki Sawada, Daisuke Shibata, Koichi Hori, Yuichiro Watanabe

    Journal of General Plant Pathology   71 ( 1 )   8 - 22   2005.2

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  • Identification of the genes expressed during spore germination of Mycosphaerella pinodes.

    J. Gen. Plant Pathol.   71 (3), 190-195.   2005

  • Pea apoplastic Cu/Zn-SOD responsive to pathogenic signal molecules

    T Kasai, K Ono, T Suzuki, K Toyoda, Y Inagaki, Y Ichinose, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   46   S94 - S94   2005

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  • Mechanism of flagellin-induced hypersensitive cell death in Arabidopsis thaliana

    Y Ishiga, YC Lin, M Marutani, F Taguchi, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   46   S94 - S94   2005

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  • Pseudomonas syringaeのべん毛を介した植物相互作用

    一瀬勇規, 田口富美子, 竹内香純, 石賀康博, 村田勝義, 加藤悦子, 丸谷瑞理, 三木隆二, 安田千裕

    日本植物病理学会植物感染生理談話会論文集   ( 40 )   13 - 24   2004.8

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  • Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii

    Hiroyuki Takahara, Gento Tsuji, Yasuyuki Kubo, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   70 ( 2 )   93 - 96   2004.4

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  • Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products

    H Matsui, G Nakamura, Y Ishiga, H Toshima, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    MOLECULAR GENETICS AND GENOMICS   271 ( 1 )   1 - 10   2004.2

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    DOI: 10.1007/s00438-003-0948-6

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  • Differential regulation of MBP kinases by a glycoproptein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea

    Uppalapati, SR, K Toyoda, Yasuhiro, I, Y Ichinose, T Shiraishi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   64 ( 1 )   17 - 25   2004.1

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  • 耐病性のシグナリングモデル,新

    分子レベルからみた植物の耐病性 (細胞工学別冊),(島本 功ら編) 秀潤社 (東京)   pp.14-17.   2004

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  • 植物の病原菌に体する受容性と抵抗性

    新版 分子レベルからみた植物の耐病性 (細胞工学別冊),(島本 功ら編) 秀潤社   pp.74-81.   2004

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  • PDR-type ABC transporter and tobacco defense response against pathogen

    Y Ichinose, D Schenke, M Sasabe, Y Inagaki, K Toyoda, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   45   S1 - S1   2004

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  • Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101

    D Schenke, M Sasabe, K Toyoda, Y Inagaki, T Shiraishi, Y Ichinose

    GENES & GENETIC SYSTEMS   78 ( 6 )   409 - 418   2003.12

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    DOI: 10.1266/ggs.78.409

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  • Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

    F Taguchi, R Shimizu, R Nakajima, K Toyoda, T Shiraishi, Y Ichinose

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   41 ( 2 )   165 - 174   2003.2

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  • Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea.

    The Scientific Reports of The Faculty of Agriculture, Okayama University.   92, in press   2003

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  • Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

    J. Gen. Plant Pathol.   69 (4) 244-249.   2003

  • Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

    J. Gen. Plant Pathol.   69 (4) 244-249.   2003

  • Role of flagella and flagellin in plant-Pseudomonas syrignae interactions. In Japan/ Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe (N. Sako, H. Yaegashi and M.-K. Yang eds.)

    Showado Co. (Saga, Japan)   pp. 158-166.   2003

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  • Phylogenetic Classification of Dof type Transcription Factors in Pea (Pisum sativum)

    Naoki Nakamura, Mizuri Marutani, Shiroh Sanematsu, Kazuhiro Toyoda, Yoshi-shige Inagaki, Tomonori Shiraishi, Yuki Ichinose

    Plant Biotechnology   20 ( 3 )   247 - 252   2003

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  • Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

    J. Gen. Plant Pathol.   69 (6) In press   2003

  • Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

    J. Gen. Plant Pathol.   69 (6) In press   2003

  • Role of flagella and flagellin in plant - Pseudomonas syringae interactions

    Y Ichinose, R Shimizu, F Taguchi, K Takeuchi, M Marutani, T Mukaihara, Y Inagaki, K Toyoda, T Shiraishi

    PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS   pp. 311-318.   311 - 318   2003

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  • Post-translational modification of flagellin determines the specificity of HR induction.

    Plant Cell Physiol.   44 (3) 342-349.   2003

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  • Flagella-mediated Interactions between Phytopathogenic Bacteria and Plants

    ICHINOSE Yuki, TAGUCHI Fumiko, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori

    KAGAKU TO SEIBUTSU   41 (8) 511-516 ( 8 )   511 - 516   2003

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    DOI: 10.1271/kagakutoseibutsu1962.41.511

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  • Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea

    Seki Hikaru, Marutani Mizuri, Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

    92, in press ( 1 )   21 - 26   2003

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  • Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

    J. Gen. Plant Pathol.   69: 33-38.   2003

  • Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

    J. Gen. Plant Pathol.   69: 33-38.   2003

  • The DfliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells.

    Mol. Genet. Genomics   269: 21-30.   2003

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  • Role of flagella and flagellin in plant-Pseudomonas syrignae interactions. In Japan/ Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe (N. Sako, H. Yaegashi and M.-K. Yang eds.)

    Showado Co. (Saga, Japan)   pp. 158-166.   2003

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  • (321)Hypersensitive Response-Inducing Factors Produced by Pseudomonas syringae (5) Specific HR-Inducing Activity of Flagellins from Several Pathovars of P. syringae Pseudomonas

    Taguchi F, Shimizu R, Takeuchi K, Ikeda Y, Inagaki Y, Shiraishi T, Ichinose Y

    Annals of the Phytopathological Society of Japan   68 ( 2 )   242 - 243   2002.8

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  • (322)Hypersensitive Response-Inducing Factors Produced by Pseudomonas syringae (6) Putative Genomic Island Involved in Flagellin Glycosylation of P. syringae

    Takeuchi K, Kawasaki T, Taguchi F, Shimizu R, Ikeda Y, Inagaki Y, Shiraishi T, Ichinose Y

    Annals of the Phytopathological Society of Japan   68 ( 2 )   243 - 243   2002.8

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  • Hypersensitive Response-Inducing Factors Produced by Pseudomonas syringae (4) Functional Analysis of Flagellin Using Flagella-Deficient Mutants(Abstracts Presented at the Meeting of the Kansai Division)

    Shimizu R, Taguchi F, Ikeda Y, Toyoda K, Shiraishi T, Inagaki Y, Ichinose Y

    Annals of the Phytopathological Society of Japan   68 ( 1 )   100 - 100   2002.4

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  • Pseudomonas syringae pv. tabaciの単量体Flagellinは,非宿主植物トマト細胞にHR誘導因子として働く

    清水鈴菜, 田口富美子, 池田陽子, 豊田和弘, 白石友紀, 稲垣善茂, 一瀬勇規

    日本植物生理学会年会要旨集   42nd   238   2002.3

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  • HR誘導性細菌エリシターflagellinのHR誘導及び病原性における翻訳後修飾の重要性

    田口富美子, 池田陽子, 清水鈴菜, 竹内香純, 稲垣善茂, 白石友紀, 一瀬勇規

    日本植物生理学会年会要旨集   42nd   238   2002.3

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  • cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene.

    FEBS Letters   518, 164-168   2002

  • Molecular cloning of cDNA for a novel pea Dof protein, PsDof1, and its DNA binding activity to the promoter of PsDof1 gene.

    SEKI Hikaru, NAKAMURA Naoki, MARUTANI Mizuri, OKABE Takeharu, SANEMATSU Shiroh, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki

    Plant Biotechnol.   19 ( 4 )   251 - 260   2002

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    A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn2+ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.

    DOI: 10.5511/plantbiotechnology.19.251

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  • Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene

    SEKI Hikaru, NAKAMURA Naoki, MARUTANI Mizuri, OKABE Takeharu, SANEMATSU Shiroh, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki

    Plant tissue culture letters   19 ( 4 )   251 - 260   2002

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    A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn2+ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.

    DOI: 10.5511/plantbiotechnology.19.251

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  • Flagellin, a constituent of a flagella filament in Pseudomonas syringae pv. tabaci, is an intense HR elicitor as a monomer molecule on nonhost tomato cells

    R Shimizu, F Taguchi, Y Ikeda, K Toyoda, T Shiraishi, Y Inagaki, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   43   S201 - S201   2002

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  • Cell wall-plasma membrane continuum is involved in transduction of pathogen signals

    K Toyoda, AM Kiba, T Kawahara, M Sugimoto, RS Uppalapati, Y Inagaki, Y Ichinose, M Yamamoto, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   43   S10 - S10   2002

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  • The importance of post-translational modification of bacterial HR-elicitor, flagellin on HR-inducing ability and pathogenicity

    F Taguchi, Y Ikeda, R Shimizu, K Takeuchi, Y Inagaki, T Shiraishi, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   43   S201 - S201   2002

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  • Biochemical and molecular biological studies on infection (Ascochyta rabiei)-induced thaumatin-like proteins from chickpea plants (Cicer arietinum L.)

    T Hanselle, Y Ichinose, W Barz

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES   56 ( 11-12 )   1095 - 1107   2001.11

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  • Generation of hydrogen peroxide is not required for harpin-induced apoptotic cell death in tobacco BY-2 cell suspension culture

    Y Ichinose, S Andi, R Doi, R Tanaka, F Taguchi, M Sasabe, K Toyoda, T Shiraishi, T Yamada

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   39 ( 9 )   771 - 776   2001.9

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    DOI: 10.1016/S0981-9428(01)01300-6

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  • Contrary operations of Box-I element of pea phenylalanine ammonia-lyase gene 1 promoter for organ-specific expression

    Y Imura, H Seki, K Toyoda, Y Ichinose, T Shiraishi, T Yamada

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   39 ( 5 )   355 - 362   2001.5

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  • Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J

    Y Ichinose, Y Hisayasu, S Sanematsu, Y Ishiga, H Seki, K Toyoda, T Shiraishi, T Yamada

    PLANT SCIENCE   160 ( 5 )   997 - 1006   2001.4

  • Bacteriophage P4282, a parasite of Ralstonia solanacearum, encodes a bacteriolytic protein important for lytic infection of its host

    H Ozawa, H Tanaka, Y Ichinose, T Shiraishi, T Yamada

    MOLECULAR GENETICS AND GENOMICS   265 ( 1 )   95 - 101   2001.3

  • Bacteriophage P4282, a parasite of Ralstonia solanacearum, encodes a bacteriolytic protein important for lytic infection of its host

    H Ozawa, H Tanaka, Y Ichinose, T Shiraishi, T Yamada

    MOLECULAR GENETICS AND GENOMICS   265 ( 1 )   95 - 101   2001.3

  • HarpinPsta from ┣DB Pseudomonas syringae (/)-┫DB pv.┣DB tabaci (/)-┫DB is defective and deficient in its gene expression and HR-inducing activity.

    J. Gen. Plant Pathol.   67 ( 2 )   116 - 123   2001

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  • Effect of harpin from four pathovars of ┣DB Pseudomonas syringae (/)-┫DB on pea defense responses.

    J. Gen. Plant Pathol.   67 ( 2 )   148 - 151   2001

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  • Effect of Harpin from Four Pathovars of Pseudomonas syringae on Pea Defense Responses (Short Communication) :

    TANAKA Rui, TAGUCHI Fumiko, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    Journal of general plant pathology : JGPP   67 ( 2 )   148 - 151   2001

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    To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)

    DOI: 10.1007/PL00013001

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  • 初期・表層シグナル伝達系と防御システム

    化学と生物   39 ( 10 )   531 - 537   2001

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  • Methyl jasmonate inhibits Harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

    Plant Cell Physiol.   42 ( 4 )   446 - 449   2001

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  • HarpinPsta from ┣DB Pseudomonas syringae (/)-┫DB pv.┣DB tabaci (/)-┫DB is defective and deficient in its gene expression and HR-inducing activity.

    TAGUCHI Fumiko, TANAKA Rui, KINOSHITA Sayuri, ICHINOSE Yuki, IMURA Yoshiyuki, ANDI Salamah, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    J. Gen. Plant Pathol.   67 ( 2 )   116 - 123   2001

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    To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P.s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin_<psta> was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P.s. pathovars in hrp-inducing medium, the truncated harpin_<psta> was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P.s. pv. tabaci. (Received August 10, 2000 ; Accepted December 21, 2000)

    DOI: 10.1007/PL00012997

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  • Gramine Increase Associated with Rapid and Transient Systemic Resistance in Barley Seedlings Induced by Mechanical and Biological Stresses

    MATSUO Hironobu, TANIGUCHI Kumiko, HIRAMOTO Tadahiro, YAMADA Tetsuji, ICHINOSE Yuki, TOYODA Kazuhiro, TAKEDA Kazuyoshi, SHIRAISHI Tomonori

    Plant and Cell Physiology   42 ( 10 )   1103 - 1111   2001

  • Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

    K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

    JOURNAL OF PHYTOPATHOLOGY   148 ( 11-12 )   633 - 636   2000.12

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  • Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

    K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

    JOURNAL OF PHYTOPATHOLOGY   148 ( 11-12 )   633 - 636   2000.12

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  • Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture

    M Sasabe, K Takeuchi, S Kamoun, Y Ichinose, F Govers, K Toyoda, T Shiraishi, T Yamada

    EUROPEAN JOURNAL OF BIOCHEMISTRY   267 ( 16 )   5005 - 5013   2000.8

  • Characterization of cDNAs encoding two glycine-rich proteins in chickpea (Cicer arietinum L.): accumulation in response to fungal infection and other stress factors

    H Cornels, Y Ichinose, W Barz

    PLANT SCIENCE   154 ( 1 )   83 - 88   2000.5

  • Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

    M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   41 ( 2 )   234 - 238   2000.2

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  • Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization

    Y Ichinose, K Tiemann, C Schwenger-Erger, K Toyoda, F Hein, T Hanselle, H Cornels, W Barz

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES   55 ( 1-2 )   44 - 54   2000.1

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  • Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack :

    IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Journal of general plant pathology : JGPP   66 ( 2 )   123 - 127   2000

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    Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

    DOI: 10.1007/PL00012932

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  • Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

    K. Toyoda, T. Kawahara, Y. Ichinose, T. Yamada, T. Shiraishi

    Journal of Phytopathology   148 ( 11-12 )   633 - 636   2000

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  • Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

    K. Toyoda, T. Kawahara, Y. Ichinose, T. Yamada, T. Shiraishi

    Journal of Phytopathology   148 ( 11-12 )   633 - 636   2000

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  • lndependent pathways leading to apoptotic cell death,oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture.(共著)

    SASABE M, TAKEUCHI K, ICHINOSE Y, TOYODA K, SHIRAISHI T, YAMADA T, KAMOUN S, GOVERS F

    European Journal of Biochemistry   267 ( 16 )   5005 - 5013   2000

  • Cytochalasin A inhibits the binding of phenylalanine ammonia-lyass mRNA to ribosomes during induction of phytoalexin in pea seedlings.(共著)

    Plant Cell Physiology   41   234 - 238   2000

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  • lmportance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression lnduced by Nonpathogen Attack.(共著)

    IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Journal of General Plant Pathol.ogy   66 ( 2 )   123 - 127   2000

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    Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

    DOI: 10.1007/PL00012932

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  • PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

    KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    Plant and cell physiology   41   s10   2000

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  • STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE :

    TANAKA Rui, TAGUCHI Tomiko, KINOSHITA Sayuri, NAKADA Hideki, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    Plant and cell physiology   41   s84   2000

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  • 植物病原菌と防御機構 (環境応答・適応の分子機構) -- (植物の環境応答と耐性のメカニズム)

    山田 哲治, 白石 友紀, 一瀬 勇規

    蛋白質核酸酵素   44 ( 15 )   2308 - 2314   1999.11

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  • Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

    A Kiba, T Takeda, T Kanemitsu, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   40 ( 9 )   978 - 985   1999.9

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  • Functional analysis of retrotransposons in pea

    H Kato, P Sriprasertsak, H Seki, Y Ichinose, T Shiraishi, T Yamada

    PLANT AND CELL PHYSIOLOGY   40 ( 9 )   933 - 941   1999.9

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  • Changes in in vivo DNA-protein interactions in pea phenylalanine ammonia-lyase and chalcone synthase gene promoter induced by fungal signal molecules

    H Seki, Y Nagasugi, Y Ichinose, T Shiraishi, T Yamada

    PLANT AND CELL PHYSIOLOGY   40 ( 1 )   88 - 95   1999.1

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  • Changes in in vivo DNA-protein interactions in pea phenylalanine ammonia-lyase and chalcone synthase gene promoter induced by fungal signal molecules

    H Seki, Y Nagasugi, Y Ichinose, T Shiraishi, T Yamada

    PLANT AND CELL PHYSIOLOGY   40 ( 1 )   88 - 95   1999.1

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  • Molecular cloning of a cDNA encoding a putative DNA-binding zinc-finger protein in pea.(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   88   25 - 30   1999

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  • Expression of the chimeric pea PSPAL2 promoter in transgenic tobacco in response to fungal ingress and injury.(共著)

    Permpong SRIPRASERTSAK, Andi SALAMAH, Yoshiyuki IMURA, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

    Annals of Phytopathological Society of Japan   65 ( 2 )   123 - 130   1999

  • Expression of the Chimeric Pea PSRAL2 Promoter in Transgenic Tobacco in Response to Fungal Ingress and Injury

    SRIPRASERTSAK Permpong, SALAMAH Andi, IMURA Yoshiyuki, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Annals of the Phytopathological Society of Japan   65 ( 2 )   123 - 130   1999

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    The 5'-upstream region of the pea phenylalanine ammonia-lyase gene2 (RSRAL2-FL, _ 2196 to +110)promoter was dissected by deleting in a series and fused to the coding region of the reporter gene encoding β-glucuronidase (GUS). The constructed chimeric promoters designated as PSPAL2-FLd1(-1486 to +110), PSPAL2-FLd2 (-966 to +110), PSPAL2-FLd3 (-594 to +110) and the full-length promoter PSPAL2-FL (-2196 to +110) were used to transform tobacco plants by the Agrobacteriummediated leaf disk method. Histochemically, GUS expression of the PSPAL2 promoter was examined in mature leaves of these transgenic tobacco leaves inoculated with pathogdnic and non pathogenic fungi. GUS expression was induced by inoculation with a non pathogen, Phytophthora capsici, as detected by a very clear expression zone around the hypersensitive response (HR) area, especially in the transformants of PSPAL2-FL and PSPAL2-FLdl. However, on leaves inoculated with a pathogen, P. nicotianae, only weak and hazy colorations were detected around the inoculation site even in the transformant of PSPAL2-FL. Moreover, wounding mature leaves with a sterile razor blade also triggered remarkable expression around the wounded sites, especially in the transformant of PSPAL2-FL. We suggest that PSPAL2 promoter expression in transgenic tobacco is induced not only by fungal ingression but also by wounding.

    DOI: 10.3186/jjphytopath.65.123

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  • Cloning and Structural Characterization of hrp Locus of Pseudomonas syringae pv. pisi.(共著)

    Hideki NAKADA, Aiko TAHARA, Masaharu HAYASHI, Rena SHIMIZU, Rui TANAKA, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

    Annals of Phytopathological Society of Japan   65 ( 2 )   147 - 152   1999

  • Tranformation of the endophyte Neotyphodium With the iaaM gene(共著)

    Ahmad YUNUS, Shinji KAWAMATA, Tadayuki SHIMANUKI, Yasuhiro MURAKAMI, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

    Annals of Phytopathological Society of Japan   65 ( 2 )   192 - 196   1999

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    DOI: 10.3186/jjphytopath.65.192

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  • Transformation of the Endophyte Neotyphodium with the iaaM Gene.

    YUNUS Ahmad, KAWAMATA Shinji, SHIMANUKI Tadayuki, MURAKAMI Yasuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Jpn. J. Phytopathol.   65 ( 2 )   192 - 196   1999

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    DOI: 10.3186/jjphytopath.65.192

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  • Functional Analysis of retrotransposons in pea.(共著)

    Plant Cell Phyiology   40   933 - 941   1999

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  • Cloning and Structural Characterization of hrp Locus of Pseudomonas syrigae pv. pisi

    NAKADA Hideki, TAHARA Aiko, HAYASHI Masaharu, SHIMIZU Rena, TANAKA Rui, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Annals of the Phytopathological Society of Japan   65 ( 2 )   147 - 152   1999

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    The hrp gene locus of Pseudomonas Syringae pv. pisi (P. s. pisi) race l was cloned in SupefCosl vector. The nucleotide sequences of hrpA, hrpB and hrpZ were determined, and the deduced amino acid sequences were compared with different races of P. s. pisi and corresponding genes in other baceria. In particular, over 99% of the similarity in HrpZ (harpin) amino acid sequences was observed among the different races of P. s. pisi. Among P syringae pathovars, very high similarity was observed, but the level of similarity was considerably lower than that of Erwinia. The HrpZ protein is rich in glycine but lacks cysteine. It is also heat tolerant as has been reported with other bacterial harpins. The carboxyl terminus of harpin also contains two directly repeated sequences of GGGLGTP and QTGT commonly found in P syringae pv. syringae. It is very interesting to note that a tetrapeptide sequence, GDET, homologous to the hexapeptide Mycosphaerella pinodes-supprescin B (SSGDET) was found in HrpZ of P. s. pisi.

    DOI: 10.3186/jjphytopath.65.147

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  • cNDA cloning and gene expression of three small GTP-binding proteins in defense response of chickpea. Reviewed

    Ichinose, Y, Toyoda, K, Barz, W

    Biochimica et Biophysica Acta   1489 ( 2-3 )   462 - 466   1999

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    DOI: 10.1016/S0167-4781(99)00201-8

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  • 植物病原菌と防御機構(共著)

    蛋白質・核酸・酵素   44   2308 - 2314   1999

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  • Molecular cloning of a cDNA encoding a putative DNA-binding zinc-finger protein in pea.(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   88   25 - 30   1999

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  • Co-purification of plasma membrane ATPase and phosphatidylinositol kinase from pea plasma membrane.(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   88   31 - 38   1999

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  • Co-purification of plasma membrane ATPase and phosphatidylinositol kinase from pea plasma membrane.(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   88   31 - 38   1999

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  • Inductions of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes.(共著)

    Plant Cell Phyiology   40   978 - 985   1999

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  • Structural analysis of a putative hypovirulent plasmid, pJTPS1, found in a spontaneous avirulent mutant of Ralstonia solanacearum.(共著)

    Rena SHIMIZU, Korehiro AKAISHI, Hideaki NEGISHI, Hiroshi TANAKA, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

    Annals of Phytopathological Society of Japan   65 ( 2 )   184 - 188   1999

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    DOI: 10.3186/jjphytopath.65.184

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  • Structural Analysis of a Putative Hypovirulent Plasmid, pJTPS1, Found in a Spontaneous Avirulent Mutant of Ralstonia solanacearum

    SHIMIZU Rena, AKAISHI Korehiro, NEGISHI Hideaki, TANAKA Hiroshi, ICHlNOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Annals of the Phytopathological Society of Japan   65 ( 2 )   184 - 188   1999

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    DOI: 10.3186/jjphytopath.65.184

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  • Plant pathogens and mechanisms of defense responses

    T. Yamada, T. Shiraishi, Y. Ichinose, K. Toyoda

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   44 ( 15 )   2308 - 2314   1999

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  • Interaction between cell wall and plasma membrane via RGD motif is implicated in plant defense responses

    A Kiba, M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   39 ( 11 )   1245 - 1249   1998.11

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  • Interaction between cell wall and plasma membrane via RGD motif is implicated in plant defense responses

    A Kiba, M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   39 ( 11 )   1245 - 1249   1998.11

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  • SPECIFIC REGULATION OF CELL WALL-BOUND ATPASE BY THE SUPPRESSOR FROM MYCOSPHAERELLA PINODES(3) -IDENTIFICATION OF ELICITOR- AND SUPPRESSOR-BINDING PROTEINS IN CELL WALL-BOUND ATPASE FRACTION FROM PEA PLANT-

    KIBA Akinori, SUGIMOTO Megumi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    39   S144 - S144   1998.5

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  • RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS II

    SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    39   S144 - S144   1998.5

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  • PURIFICATION AND ANALYSIS OF ELICITOR BINDING-PROTEIN IN CELL WALL FRACTION FROM PEA

    SUGIMOTO Megumi, KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    39   S143 - S143   1998.5

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  • In vivo FOOTPRINTING ANALYSIS OF cis-REGULATORY ELEMENTS INVOLVED IN THE ELICITOR-MEDIATED ACTIVATION AND SUPPRESSOR-MEDIATED DEACTIVATION OF PEA PAL AND CHS

    SEKI Hikaru, ICHINOSE Yuki, NAGASUGI Yumi, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    39   S43 - S143   1998.5

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  • ISOLATION AND CHARACTERIZATION OF ELICITOR-RESPONSIVE GENES IN PEA

    ICHINOSE Yuki, SANEMATSU Shiroh, ENDOH Ai, OKABE Takeharu, HISAYASU Yumiko, SEKI Hikaru, SHIRAISHI Tomonori, TOYODA Kazuhiro, YAMADA Tetsuji

    39   S143 - S143   1998.5

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  • Cis-regulatory elements and trans-acting factors involved in the activation of a member of elicitor-responsive pea chalcone synthase gene family, PSCHS2(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   87   91 - 97   1998

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  • Phosphorylation of phosphatidylinositols and production of lysophospholipid in pea plasma membrane are coordinately regulated by elicitor-and suppressor from Mycosphaerella pinodes(共著)

    Toyoda Kazuhiro, Koyama Masashi, Mizukoshi Rumi, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

    Scientific Reports of the Faculty of Agriculture Okayama University   87 ( 1 )   109 - 116   1998

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  • Cis-regulatory elements and trans-acting factors involved in the activation of a member of elicitor-responsive pea chalcone synthase gene family, PSCHS2(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   87   91 - 97   1998

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  • Transformation of mutualistic fungal Acremonium endophyte(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   87   99 - 107   1998

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  • Phosphorylation of Phosphatidylinositols and Production of Lysophospholipid in Pea Plasma Membrane Are Coordinately Regulated by Elicitor and Suppressor from Mycosphaerella pinodes

    Toyoda Kazuhiro, Koyama Masashi, Mizukoshi Rumi, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

    87 ( 1 )   109 - 116   1998

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  • Transformation of mutualistic fungal Acremonium endophyte(共著)

    Scientific Reports of the Faculty of Agriculture Okayama University   87   99 - 107   1998

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  • Elevation of diacylglycerol during the early stage of elictitor-signal transduction in pea(Pisum sativum).(共著)

    Kazuhiro TOYODA, Tomoharu KAWAHARA, Rumi MIZUKOSHI, Masashi KOYAMA, Yuki ICHINOSE, Tetsuji YAMADA, Tomonori SHIRAISHI

    Annals of Phytopathological Society of Japan   64 ( 5 )   485 - 487   1998

  • Elevation of Diacylglycerol during the Early Stage of Elicitor-signal Transduction in Pea (Pisum sativum)

    TOYODA Kazuhiro, KAWAHARA Tomoharu, MIZUKOSHI Rumi, KOYAMA Masashi, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    Annals of the Phytopathological Society of Japan   64 ( 5 )   485 - 487   1998

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    DOI: 10.3186/jjphytopath.64.485

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  • Plant cell wall my recognize pathogenic microorganisms

    Tomonori SHIRAISHI, Akinori KIBA, Kazuhiro TOYODA, Yuki ICHINOSE, Tetsuji YAMADA

    KASEAA   36 ( 4 )   226 - 233   1998

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    DOI: 10.1271/kagakutoseibutsu1962.36.226

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  • Functional analysis of the putative cis-elements involved in activation by elicitor or UV and deactivation by suppressor in the promoter of a pea gene for phenylalanine ammonia-lyase (PSPAL1)

    Y Murakami, Y Ichinose, T Shiraishi, T Yamada

    PLANT AND CELL PHYSIOLOGY   38 ( 12 )   1403 - 1408   1997.12

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  • Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules

    A Kiba, C Miyake, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PHYTOPATHOLOGY   87 ( 8 )   846 - 852   1997.8

  • Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco

    S Kawamata, K Shimoharai, Y Imura, M Ozaki, Y Ichinose, T Shiraishi, H Kunoh, T Yamada

    PLANT AND CELL PHYSIOLOGY   38 ( 7 )   792 - 803   1997.7

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  • Putative cis-regulatory elements for elicitor-mediated transcriptional activation and molecular evolution of the pea chalcone synthase genes.

    Y Ichinose, H Seki, M Ito, T Shiraishi, T Yamada

    PLANT PHYSIOLOGY   114 ( 3 )   1175 - 1175   1997.7

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  • Specific regulation of cell wall-bound ATPase and peroxidase by the suppressor for defense response.

    A Kiba, A Inata, C Miyake, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT PHYSIOLOGY   114 ( 3 )   1176 - 1176   1997.7

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  • Con A-binding protein in plasma membranes may participate in signal-transduction cascades that lead to defense responses in pea.

    K Toyoda, M Sugimoto, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

    PLANT PHYSIOLOGY   114 ( 3 )   1502 - 1502   1997.7

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  • Association between ion fluxes and defense responses in pea and cowpea tissues

    M Amano, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   38 ( 6 )   698 - 706   1997.6

  • Molecular evolution and functional relevance of the chalcone synthase genes of pea

    M Ito, Y Ichinose, H Kato, T Shiraishi, T Yamada

    MOLECULAR & GENERAL GENETICS   255 ( 1 )   28 - 37   1997.6

  • dad-1, a putative programmed cell death suppressor gene in rice

    Y Tanaka, T Makishima, M Sasabe, Y Ichinose, T Shiraishi, T Nishimoto, T Yamada

    PLANT AND CELL PHYSIOLOGY   38 ( 3 )   379 - 383   1997.3

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  • RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS -SIGNIFICANCE OF CELL WALL-

    SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, KATO Toshiaki, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    38   s58   1997.3

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  • PLANT CELL WALL - THE PRIMARY APPARATUS RECOGNIZING AND RESPONDING TO FUNGAL SIGNALS

    SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki, KIBA Akinori

    38   s11   1997.3

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  • THE SIGNIFICANCE OF ACTIVE OXYGEN SPEACIES IN ELICITATION OF DEFENSE RESPONSES IN PEA PLANTS

    INATA Atsuko, FUJII Masahiro, KIBA Akinori, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    38   s92   1997.3

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  • EFFECTS OF SYNTHETIC GLYCOPEPTIDE-ELICITORS ON DEFENSE RESPONSES OF PEA PLANTS

    KIBA Akinori, INATA Atsuko, TAKEDA Tadahiro, KANEMITSU Takuya, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    38   s93   1997.3

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  • Combined effects of multiple cis-acting elements in elicitor-mediated activation of PSCHS1 gene

    H Seki, Y Ichinose, M Ito, T Shiraishi, T Yamada

    PLANT AND CELL PHYSIOLOGY   38 ( 1 )   96 - 100   1997.1

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  • Orthovanadate induces phytoalexiu production in pea suspension-cultured cells(共著)

    Kawahara Tomoharu, Toyoda Kazuhiro, Kiba Akinori, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

    Scientific Reprots of the Fawlty of Agriculture OKAYAMA UNIVERSITY   86   33 - 41   1997

  • Association between ion fluxes and defense responses in pea and cowpea tissues(共著)

    Plant Cell Physiology   38 ( 6 )   698 - 706   1997

  • Temporal and spatial pattern of expression of the pea phenylalanine ammoria-lyase gene 1 promoter in transgenic tobacco(共著)

    Plant Cell Physiology   38 ( 7 )   792 - 803   1997

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  • The Effects of Expression of Procaryotic-type iaa Genes in Agrobacterium tumefaciems on Plant Tumorigenicity.(共著)

    Yasuhiro MURAKAMI, Tomoki NISHINO, Tsuguyoshi TAIRA, Ahmad YUNUS, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

    Annals of the Phytopathological Society of Japan   63 ( 5 )   377 - 380   1997

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    DOI: 10.3186/jjphytopath.63.377

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  • The Effects of Expression of Procaryotic-type iaa Genes inAgrobacterium tumefaciens on Plant Tumorigenicity.

    MURAKAMI Yasuhiro, NISHINO Tomoki, TAIRA Tsuguyoshi, YUNUS Ahmad, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Jpn. J. Phytopathol.   63 ( 5 )   377 - 380   1997

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    DOI: 10.3186/jjphytopath.63.377

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  • Orthovanadate Induces Phytoalexin Production in Pea Suspension-Cultured Cells

    Kawahara Tomoharu, Toyoda Kazuhiro, Kiba Akinori, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

    Scientific reports of the Faculty of Agriculture, Okayama University   86   33 - 41   1997

  • dad-1, a putative programmed cell death suppressor gene in rice(共著)

    Plant Cell Physiology   38 ( 3 )   379 - 383   1997

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  • Mechanisms of the plant disease resistance-Focusing on the suppressor-(共著)

    32 ( 1 )   49 - 59   1997

  • 植物の病害抵抗性機構-特にサプレッサーについて-

    植物の化学調節   32 ( 1 )   49 - 59   1997

  • Mechanisms of the plant disease resistance-Focusing on the suppressor-(共著)

    32 ( 1 )   49 - 59   1997

  • The role of suppressors in determining host-parasite specificities in plant cells

    T Shiraishi, T Yamada, Y Ichinose, A Kiba, K Toyoda

    INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 172   172   55 - 93   1997

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  • Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsChs1) in pea

    H Seki, Y Ichinose, H Kato, T Shiraishi, T Yamada

    PLANT MOLECULAR BIOLOGY   31 ( 3 )   479 - 491   1996.6

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  • Specific response of partially purified cell wall-bound ATPases to fungal suppressor

    A Kiba, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   37 ( 2 )   207 - 214   1996.3

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  • REGULATION OF THE GENE ENCODING PHENYLALANINE AMMONIA-LYASE OF PEA IN TRANSGENIC TOBACCO

    IMURA Yoshiyuki, MORIKAWA Akiko, MICHAELZACK Dalius, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    37   54 - 54   1996.3

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  • MOLECULAR EVOLUTION OF DEFENSE RELATED CHALCONE SYNTHASE GENES IN PEA

    ITOH Masayuki, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    37   54 - 54   1996.3

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  • Suppression of ATPase Activity in Pea Cells during Infection by a Compatible Race of Pseudomonas syringae pv. pisi

    SHIMOTSUMA Natsumi, HAYASHI Masaharu, MALIK Kamal A., ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Annals of the Phytopathological Society of Japan   62 ( 5 )   498 - 501   1996

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    DOI: 10.3186/jjphytopath.62.498

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  • Species-specific suppression of superoxide-anion generation of surfaces of pea leaves by the suppressor from Mycosphaerella pinodes(共著)

    Annals of the Phytopathological Society of JAPAN   62 ( 5 )   508 - 512   1996

  • Species-specific Suppression of Superoxide-anion Generation on Surfaces of Pea Leaves by the Suppressor from Mycosphaerella pinodes

    KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    Annals of the Phytopathological Society of Japan   62 ( 5 )   508 - 512   1996

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    DOI: 10.3186/jjphytopath.62.508

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  • Analysis of cis-regulatory elements involued in the activation of a member of chalcoue synthase gene family (PsCHS1) in pea(共著)

    Plant Molecular Biology   1996

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  • Suppression of ATPase activity in pea cells during infecfion by a compatible race of Pseudomouas syriugae pv. pisi(共著)

    SHIMOTSUMA Natsumi, HAYASHI Masaharu, MALIK Kamal A., ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Annals of Phytopathological Society of Japan   62 ( 5 )   498 - 501   1996

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    DOI: 10.3186/jjphytopath.62.498

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  • EXPRESSION OF HETEROLOGOUS PAL GENE IN MUSKMELON (CUCUMIS-MELO L CV BIRDIE)

    NM SALEH, SH TAN, Y ICHINOSE, T YAMADA

    ASIA-PACIFIC JOURNAL OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   3 ( 3 )   206 - 214   1995.9

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  • EXPRESSION OF HETEROLOGOUS PAL GENE IN MUSKMELON (CUCUMIS-MELO L CV BIRDIE)

    NM SALEH, SH TAN, Y ICHINOSE, T YAMADA

    ASIA-PACIFIC JOURNAL OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   3 ( 3 )   206 - 214   1995.9

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  • PLANT-LECTINS INDUCE THE PRODUCTION OF A PHYTOALEXIN IN PISUM-SATIVUM

    K TOYODA, K MIKI, Y ICHINOSE, T YAMADA, T SHIRAISHI

    PLANT AND CELL PHYSIOLOGY   36 ( 5 )   799 - 807   1995.7

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  • SPECIFIC-INHIBITION OF CELL WALL-BOUND ATPASES BY FUNGAL SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

    A KIBA, K TOYODA, Y ICHINOSE, T YAMADA, T SHIRAISHI

    PLANT AND CELL PHYSIOLOGY   36 ( 5 )   809 - 817   1995.7

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  • PLANT-LECTINS INDUCE THE PRODUCTION OF A PHYTOALEXIN IN PISUM-SATIVUM

    K TOYODA, K MIKI, Y ICHINOSE, T YAMADA, T SHIRAISHI

    PLANT AND CELL PHYSIOLOGY   36 ( 5 )   799 - 807   1995.7

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  • SPECIFIC-INHIBITION OF CELL WALL-BOUND ATPASES BY FUNGAL SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

    A KIBA, K TOYODA, Y ICHINOSE, T YAMADA, T SHIRAISHI

    PLANT AND CELL PHYSIOLOGY   36 ( 5 )   809 - 817   1995.7

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  • ESTABLISHMENT OF A SYSTEM FOR THE TRANSIENT EXPRESSION OF GUS GENE IN ELECTROPORATED MUSKMELON (CUCUMIS-MELO L) PROTOPLASTS

    SH TAN, NM SALEH, ZC ALANG, T YAMADA, Y ICHINOSE, K KAWAZU

    ASIA-PACIFIC JOURNAL OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   3 ( 2 )   156 - 160   1995.6

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  • A SUPPRESCIN FROM A PHYTOPATHOGENIC FUNGUS DEACTIVATES TRANSCRIPTION OF A PLANT DEFENSE GENE ENCODING PHENYLALANINE AMMONIA-LYASE

    M WADA, H KATO, K MALIK, P SRIPRASERTSAK, Y ICHINOSE, T SHIRAISHI, T YAMADA

    JOURNAL OF MOLECULAR BIOLOGY   249 ( 3 )   513 - 519   1995.6

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    DOI: 10.1006/jmbi.1995.0313

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  • CHARACTERIZATION OF NUCLEAR FACTORS FOR ELICITOR-MEDIATED ACTIVATION OF THE PROMOTER OF THE PEA PHENYLALANINE AMMONIA-LYASE GENE

    H KATO, M WADA, K MURAYA, K MALIK, T SHIRAISHI, Y ICHINOSE, T YAMADA

    PLANT PHYSIOLOGY   108 ( 1 )   129 - 139   1995.5

  • RELATIONSHIP BETWEEN DEFENSE RESPONSESAND ION FLUX

    AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    36   S119   1995.3

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  • TRANSCRIPTIONAL REGULATION OF THE GENEENCODING CHALCONE SYNTHASE IN PEA BY FUNGAL ELICITOR

    SEKI Hikaru, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    36   S120   1995.3

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  • REGULATION OF PLANT DEFENSE RESPONSES BY SIGNALS FROM FUNGAL PATHOGENS

    T SHIRAISHI, T YAMADA, Y ICHINOSE

    NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY   69 ( 2 )   174 - 177   1995.2

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    DOI: 10.1271/nogeikagaku1924.69.174

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  • THE RELATIONSHIP BETWEEN SYSTEMIC RESISTANCE INDUCED BY PRUNING AND ACCUMULATION OF ANTIFUNGAL SUBSTANCES IN BARLEY SEEDLINGS

    T HIRAMOTO, N ABE, R TOBIMATSU, T SHIRAISHI, H OKU, T YAMADA, Y ICHINOSE

    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   143 ( 1 )   43 - 46   1995.1

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  • SIGNAL MOLECULES INDUCING SYSTEMIC RESISTANCE AND SUSCEPTIBILITY IN BARLEY SEEDLINGS

    T HIRAMOTO, R TOBIMATSU, N ABE, T SHIRAISHI, H OKU, T YAMADA, Y ICHINOSE

    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   143 ( 1 )   47 - 51   1995.1

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  • Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1. (共著)

    KATO H, WADA M, MURAYA K, MALIK K, SHIRAISHI T, ICHINOSE Y, YAMADA T

    Plant Physiology   108 ( 1 )   129 - 139   1995

  • Suppression of the activation of chitinase and β-1, 3-glucanase in pea epicotyls by endogenous suppressor from ┣DBPisum sativum(/)-┫DB. (共著)

    Annals of the Phytopathological Society of Japan   1995

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  • Regulation of plant defense responses by signals from fangal pathogeus

    Tomonori Shiraishi, Tetsuji Yamada, Yuki Ichinose

    Nippon N(]E87C7[)geikagaku Kaishi   69 ( 2 )   174 - 177   1995

  • 病原菌シグナルと植物の防御応答 共著

    白石 友紀, 山田 哲治, 一瀬 勇規

    日本農芸化学会誌   69 ( 2 )   174 - 177   1995

  • H+ - translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppnessor from Mycosphaerella pinodes(共著)

    Annals of Phytopathological Society of Japan   61   134 - 136   1995

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  • Supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encodig phenylalanine ammonia-lyase. (共著)

    Journal of Molecular Biology   1995

  • Signal molecules inducing systemic resistance and susceptibility in barley seedling. (共著)

    Journal of Phytopathology   143   1995

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  • The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedlings. Reviewed

    Hiramoto, T, Abe, N, Tobimatsu, R, Shiraishi, T, Oku, H, Yamada, T, Ichinose, Y

    143   47 - 51   1995

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  • Suppression of the Activation of Chitinase and β-1,3-Glucanase in Pea Epicotyls by Endogenous Suppressor from Pisum sativum

    NASU Kimio, YOSHIOKA Hirofumi, ICHINOSE Yuki, YAMADA Tetsuji, OKU Hachiro, SHIRAISHI Tomonori

    Annals of the Phytopathological Society of Japan   61 ( 1 )   13 - 17   1995

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    Healthy pea plants contain a factor, as called an endogenous suppressor, which suppresses the accumulation of pisatin induced by the elicitor from a pea pathogen, Mycosphaerella pinodes. The effects of the endogenous suppressor on the activation of pathogenesis-related proteins (PR-proteins) such as chitinase and β-1,3-glucanase were examined. The fungal elicitor induced the activations of these enzymes in pea, kidney bean and soybean, however, the concomitant presence of the endogenous suppressor with the fungal elicitor resulted in suppression of activations of these enzymes in only pea but not in kidney bean or soybean. In contrast, the endogenous suppressor alone rather activated these enzymes in kidney bean and soybean to which M. pinodes is nonpathogenic. Thus, the action of endogenous suppressor on the activation of PR-proteins is species-specific as well as that on the production of phytoalexins. Together with our previous report, these findings show that the pea endogenous suppressor may have a potential to block the general resistance of its producer and that it is quite similar to the exogenous suppressor from M. pinodes with respect to the biological activities.

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    Other Link: http://agriknowledge.affrc.go.jp/RN/2010520740

  • H+ - translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppnessor from Mycosphaerella pinodes(共著)

    Annals of Phytopathological Society of Japan   61   134 - 136   1995

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  • SUPPRESSORS - DETERMINANTS OF SPECIFICITY PRODUCED BY PLANT-PATHOGENS

    T SHIRAISHI, T YAMADA, K SAITOH, T KATO, K TOYODA, H YOSHIOKA, HM KIM, Y ICHINOSE, M TAHARA, H OKU

    PLANT AND CELL PHYSIOLOGY   35 ( 8 )   1107 - 1119   1994.12

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  • Suppressor produced by a pea pathogen, ┣DBM(/)-┫DB. pinodes. (共著)

    Grain Legumes   4   22 - 23   1994

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  • Suppressor produced by a pea pathogen, ┣DBM(/)-┫DB. pinodes. (共著)

    Grain Legumes   4   22 - 23   1994

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  • RAPID CHANGES IN POLYPHOSPHOINOSITIDE METABOLISM IN PEA IN RESPONSE TO FUNGAL SIGNALS

    K TOYODA, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   34 ( 5 )   729 - 735   1993.7

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  • ORGANIZATION OF THE GENES ENCODING CHALCONE SYNTHASE IN PISUM-SATIVUM

    C AN, Y ICHINOSE, T YAMADA, Y TANAKA, T SHIRAISHI, H OKU

    PLANT MOLECULAR BIOLOGY   21 ( 5 )   789 - 803   1993.3

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  • ORGANIZATION OF THE GENES ENCODING CHALCONE SYNTHASE IN PISUM-SATIVUM

    C AN, Y ICHINOSE, T YAMADA, Y TANAKA, T SHIRAISHI, H OKU

    PLANT MOLECULAR BIOLOGY   21 ( 5 )   789 - 803   1993.3

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  • MOLECULAR-CLONING OF PHENYLALANINE AMMONIA-LYASE CDNA FROM PISUM-SATIVUM

    S KAWAMATA, T YAMADA, Y TANAKA, P SRIPRASERTSAK, H KATO, Y ICHINOSE, H KATO, T SHIRAISHI, H OKU

    PLANT MOLECULAR BIOLOGY   20 ( 1 )   167 - 170   1992.10

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  • PHENYLALANINE AMMONIA-LYASE GENES FROM PISUM-SATIVUM - STRUCTURE, ORGAN-SPECIFIC EXPRESSION AND REGULATION BY FUNGAL ELICITOR AND SUPPRESSOR

    T YAMADA, Y TANAKA, P SRIPRASERTSAK, H KATO, T HASHIMOTO, S KAWAMATA, Y ICHINOSE, H KATO, T SHIRAISHI, H OKU

    PLANT AND CELL PHYSIOLOGY   33 ( 6 )   715 - 725   1992.9

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  • AN ENDOGENOUS SUPPRESSOR OF THE DEFENSE RESPONSE IN PISUM-SATIVUM

    K NASU, T SHIRAISHI, H YOSHIOKA, N HORI, Y ICHINOSE, T YAMADA, H OKU

    PLANT AND CELL PHYSIOLOGY   33 ( 5 )   617 - 626   1992.7

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  • 2-SUPPRESSORS, SUPPRESCINS-A AND SUPPRESCINS-B, SECRETED BY A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

    T SHIRAISHI, K SAITOH, HM KIM, T KATO, M TAHARA, H OKU, T YAMADA, Y ICHINOSE

    PLANT AND CELL PHYSIOLOGY   33 ( 5 )   663 - 667   1992.7

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  • REGULATION OF POLYPHOSPHOINOSITIDE METABOLISM IN PEA PLASMA-MEMBRANES BY ELICITOR AND SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

    K TOYODA, T SHIRAISHI, H YOSHIOKA, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   33 ( 4 )   445 - 452   1992.6

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  • ENDOGENOUS ELICITOR PRESENT IN BARLEY-SEEDS .2. MECHANISMS OF THE INDUCTION OF THE RESISTANCE IN BARLEY LEAVES TO ERYSIPHE-GRAMINIS

    T HIRAMOTO, R TOBIMATSU, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   135 ( 2 )   167 - 176   1992.6

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  • TRANSIENT EXPRESSION IN ELECTROPORATED PEA PROTOPLASTS - ELICITOR RESPONSIVENESS OF A PHENYLALANINE AMMONIA-LYASE PROMOTER

    T HASHIMOTO, T YAMADA, A TADA, S KAWAMATA, Y TANAKA, P SRIPRASERTSAK, Y ICHINOSE, H KATO, S IZUTSU, T SHIRAISHI, H OKU, Y OHTSUKI

    PLANT CELL REPORTS   11 ( 4 )   183 - 187   1992.5

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  • MOLECULAR-CLONING OF CHALCONE SYNTHASE CDNAS FROM PISUM-SATIVUM

    Y ICHINOSE, S KAWAMATA, T YAMADA, CC AN, T KAJIWARA, T SHIRAISHI, H OKU

    PLANT MOLECULAR BIOLOGY   18 ( 5 )   1009 - 1012   1992.3

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  • ORTHOVANADATE SUPPRESSES ACCUMULATION OF PHENYLALANINE AMMONIA-LYASE MESSENGER-RNA AND CHALCONE SYNTHASE MESSENGER-RNA IN PEA EPICOTYLS INDUCED BY ELICITOR FROM MYCOSPHAERELLA-PINODES

    H YOSHIOKA, T SHIRAISHI, S KAWAMATA, K NASU, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   33 ( 2 )   201 - 204   1992.3

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  • Molecular cloning of phenylalanine ammonia-lyase cDNA from Pisum sativum 共著

    Plant Molecular Biology   20,167-170   1992

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  • Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthovanadate and a suppressor from Mycosphae-rella pinodes 共著

    Annals of the Phytopathological Society of JAPAN   58,405-410   1992

  • Suppression of the Activation of Chitinase and β-1,3-Glucanase in Pea Epicotyls by Orthovanadate and a Suppressor from Mycosphaerella pinodes

    YOSHIOKA Hirofumi, SHIRAISHI Tomonori, NASU Kimio, YAMADA Tetsuji, ICHINOSE Yuki, OKU Hachiro

    Annals of the Phytopathological Society of Japan   58,405-410 ( 3 )   405 - 410   1992

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    A pea pathogen, Mycosphaerella Pinodes, secretes both an elicitor and a suppressor in its pycnospore germination fluid. The effects of the elicitor, the suppressor and orthovanadate, an inhibitor of P-type ATPase, on the activation of pathogenesis-related proteins such as enclochitinase and β-1,3-glucanase were examined. The elicitor induced the activation of these enzymes in pea, soybean and kidney bean. Such activation in 3 plant species tested was suppressed by the concomitant presence of 1 mM orthovanadate with the elicitor. The suppressor, however, suppressed the activation in only pea plants but did not in nonhost plants of M. pinodes. These results suggest that the inhibition of the ATPase in plasma membranes highly correlates to the suppression of the activation of endo-chitinase and β-1,3-gltlcanase.

    DOI: 10.3186/jjphytopath.58.405

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  • An endogenous suppressor of the defense response in Pisum sativum 共著

    Plant Cell Physiology   33,617-626   1992

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  • Endogenous elicitor present in barley seeds II. Mechanisms of the induction of the resistance in barley leaves to Erysiphe graminis 共著

    Journal of Phytopathology   135,167-176   1992

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  • A Reporter Gene Expression Under the Control of a Pea Phenylalanine Ammonia Lyase-gene Promoter

    Yamada Tetsuji, Hashimoto Tadaaki, Sriprasertsak Permpong

    Scientific reports of the Faculty of Agriculture, Okayama University   80,51-60   51 - 60   1992

  • TWO suppressors, suppresciws A and B secreted by a pea pathogen, Mycosphaerella pinodes 共著

    Plant Cell Physiology   33,663-667   1992

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  • TWO suppressors, suppresciws A and B secreted by a pea pathogen, Mycosphaerella pinodes 共著

    Plant Cell Physiology   33,663-667   1992

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  • Molecular cloning of chalcone synthase cDNA frow Pisum sativum 共著

    Plant Molecular Biology   18,1009-1012   1992

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  • A reporter gene expression under the control of a pea phenylalanine ammonia-lyase gene promoter(共著)

    Yamada Tetsuji, Hashimoto Tadaaki, Sriprasertsak Permpong

    Scientific Reports of the Faculty of Agriculture Okayama University   80,51-60   51 - 60   1992

  • Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter. International journal

    T Hashimoto, T Yamada, A Tada, S Kawamata, Y Tanaka, P Sriprasertsak, Y Ichinose, H Kato, S Izutsu, T Shiraishi, H Oku, Y Ohtsuki

    Plant cell reports   11,183-187 ( 4 )   183 - 7   1992

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    High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

    DOI: 10.1007/BF00232529

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  • INHIBITION OF ATPASE ACTIVITY IN PEA PLASMA-MEMBRANES INSITU BY A SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

    T SHIRAISHI, M ARAKI, H YOSHIOKA, KOBAYASHI, I, T YAMADA, Y ICHINOSE, H KUNOH, H OKU

    PLANT AND CELL PHYSIOLOGY   32 ( 7 )   1067 - 1075   1991.10

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  • エンドウ動的遺伝子の発現機構. Invited

    山田哲治, 白石友紀, 一瀬勇規, 奥 八郎, 川又伸治, 田中良和, Spirasertsak, P, 安 成才, 吉岡博文, 橋本忠明

    生物相互間の認識機構   27   56-65   1991

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  • SUPPRESSION OF PISATIN PRODUCTION AND ATPASE ACTIVITY IN PEA PLASMA-MEMBRANES BY ORTHOVANADATE, VERAPAMIL AND A SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

    H YOSHIOKA, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   31 ( 8 )   1139 - 1146   1990.12

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  • IDENTIFICATION OF MOUSE ENDODERMAL CYTOSKELETAL PROTEIN-C (ENDOC) WITH CYTOKERATIN NO-19

    Y ICHINOSE, M NOZAKI, T MORITA, A MATSUSHIRO

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   167 ( 2 )   644 - 647   1990.3

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    DOI: 10.1016/0006-291X(90)92073-9

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  • Suppression of pisatin production and ATPase activity in pea plasma membranes by orthovanadate, verapamil and a suppressor from Mycosphaerella pinodes 共著

    Plant Cell Physiology   31,1139-1146   1990

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  • MOLECULAR-CLONING AND CHARACTERIZATION OF CDNA-ENCODING MOUSE CYTOKERATIN-NO-19

    Y ICHINOSE, K HASHIDO, H MIYAMOTO, T NAGATA, M NOZAKI, T MORITA, A MATSUSHIRO

    GENE   80 ( 2 )   315 - 323   1989.8

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  • Molecular cloning and characerization of cDNA encoding for mouse cytokeratin no. 19 共著

    Gene   80,315-323   1989

  • NUCLEOTIDE-SEQUENCE AND STRUCTURE OF THE MOUSE CYTOKERATIN ENDOB GENE

    Y ICHINOSE, T MORITA, FY ZHANG, S SRIMAHASONGCRAM, MLC TONDELLA, M MATSUMOTO, M NOZAKI, A MATSUSHIRO

    GENE   70 ( 1 )   85 - 95   1988.10

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  • NUCLEOTIDE-SEQUENCE OF MOUSE ENDOA CYTOKERATIN CDNA REVEALS POLYPEPTIDE CHARACTERISTICS OF THE TYPE-II KERATIN SUBFAMILY

    T MORITA, MLC TONDELLA, Y TAKEMOTO, K HASHIDO, Y ICHINOSE, M NOZAKI, A MATSUSHIRO

    GENE   68 ( 1 )   109 - 117   1988.8

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  • Nucleotide sequence and structure of a mouse cytokeratin endoB gene 共著

    Gene   70,85-95   1988

  • Regulation of pisatin biosynthesis in pea leaves by elicitor and suppressor produced by Mycosphaerella pinodes

    HIRAMATSU M.

    Ann. Phytopath. Soc. Jpn.   52,53-58   53 - 58   1986

  • Regulation of pisatin biosynthesis in pea leaves by elicitor and suppressor produced by Mycosphaerella pinodes 共著

    Annals of the Phytopathological Society of JAPAN   52,53-58   1986

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Works

  • Flagellar motility and structure in phytopathogenic bacteria

    2004

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  • 植物病原細菌のべん毛糖鎖構造の解析

    2001

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  • Glycosyl structure of flagellin in phytopathogenic bacteria

    2001

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  • 植物の生体防御遺伝子の分子生物学的解析

    1996

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  • Molecular study of plant defense genes

    1996

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Awards

  • 平成21年度日本植物病理学会 学会賞受賞

    2009  

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    Country:Japan

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  • 日本植物生理学会論文賞

    1994  

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    Country:Japan

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Research Projects

  • 植物シグナルによる植物病原細菌の感染行動と病原力遺伝子の発現制御機構

    Grant number:22H0234814  2022.04 - 2026.03

    日本学術振興会  科研費  基盤研究(B)

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  • Regulation of infectious behavior and virulence-related genes in phytopathogenic bacteria by plant-derived signals

    Grant number:22H02348  2022.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    一瀬 勇規, 松井 英譲

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    Grant amount:\17420000 ( Direct expense: \13400000 、 Indirect expense:\4020000 )

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  • Regulation of infectious behavior and virulence-related genes in phytopathogenic bacteria by plant-derived signals

    Grant number:23K23613  2022.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    一瀬 勇規, 松井 英譲

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    Grant amount:\17420000 ( Direct expense: \13400000 、 Indirect expense:\4020000 )

    植物病原菌の病原性における走化性の重要性を明らかにするため、高い運動能と病原力を有するタバコ野火病菌(Pseudomonas syringae pv tanbaci 6605)(Pta6605)をモデル病原細菌として解析した。Pta6605は、タバコなどの宿主植物のアポプラスト液だけでなく、トマトなどの非宿主植物のアポプラスト液に対しても正の走化性を示した。また、タバコのアポプラスト液にはPta6605が正の走化性を示すタンパク質性アミノ酸やγ-アミノ酪酸(GABA)が豊富であること、リンゴ酸などの有機酸も豊富であることを明らかにした。
    走化性の検定にはキャピラリー中の誘引物質に誘引された細菌数を測定する量的キャピラリーアッセイと、誘引物質をマイクロキャピラリーに充填し、集まってくる細菌を顕微鏡下で観察するマイクロキャピラリーアッセイが用いられている。それぞれの方法には長短があるが、新たに走化性検定に適したマイクロデバイスを作成し、より簡便に多くのサンプルを観察できるマイクロデバイス法を開発中である。
    一方、Pta6605の有する54個の走化性受容体遺伝子について、単独欠損変異株の作出に成功したため、網羅的な受容体スクリーニングにも対応可能な状態である。これらの走化性受容体は、それぞれのリガンド結合ドメインなどの構造や局在などから細かなサブグループに分類できた。現在、Pta6605が正の走化性を示したギ酸、硝酸ナトリウムに対する受容体の同定に取り組んでいる。
    これらの実績として、令和5年度には国際雑誌に原著論文1報を報告した他、国際学会で1回、国内学会で4回の発表を行った。

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  • Regulation of virulence-related gene expression by Gas/Rsm-mediated quorum sensing in plant pathogenic bacteria

    Grant number:19H02956  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Ichinose Yuki

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    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

    Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) induces the expression of rsmX and rsmY at high cell density, which are small non-coding RNAs (snRNA) and is thought to control translation. The single snRAN deletion mutants we generated showed no remarkable difference from wild type (WT) strain. However, if we delete rsmX1, 2, 3, 4, 5 together, the mutant reduced the expression of flagella-related genes and type 3 secretion system (T3SS)-related genes. Furthermore, the mutant reduced the levels of swimming ability and virulence to host Arabidopsis thaliana. These results indicate that at low cell density RsmA (translation regulator) captures flagella- and T3SS-related mRNA and inhibit their expression, at high cell density newly synthesized rsmX RNA binds RsmA and activates these translation.

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  • Role of multi-drug efflux pump transporters on virulence in phytopathogenic bacteria

    Grant number:16K14861  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Ichinose Yuki

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    Grant amount:\3770000 ( Direct expense: \2900000 、 Indirect expense:\870000 )

    We investigated the role of resistance-nodulation-division (RND) superfamily, MexAB-OpeM and MexEF-OprN on virulence in Pseudomonas syringae pv. tabaci (Pta) 6605. The sensitivity to plant-derived antimicrobial compounds was investigated in mexB and mexF mutant strains. Bacterial growth was inhibited by acetovanillon, catechol and coumarin in mexB and mexF mutant strains, and virulence of these mutant strains are impaired. The mexAB-oprM was constitutively expressed and activated in mexF mutant, and silenced expression of mexEF-oprN in WT strain was activated in mexB mutant. These results indicate that MexAB-OprM and MexEF-OprN in Pta6605 may exclude acetovanillone, catechol, and coumarin, and MexAB-OprM and MexEF-OprN complemented their function each other. When mexT was overexpressed in Pta6605 WT strain, the expression of mexEF-oprN was activated and accumulation of AHL was cancelled. This result indicates that MexEF-OprN is a negative regulator of AHL accumulation in Pta6605.

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  • Analysis of quorum sensing-meditated regulation of T3SS gene expression

    Grant number:15H04458  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ICHINOSE Yuki

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    Grant amount:\16640000 ( Direct expense: \12800000 、 Indirect expense:\3840000 )

    The mechanisms of acyl-homoserinelactone (AHL)-mediated quorum sensing (QS) in Pseudomonas syringae was investigated. Recombinant PsyR of P. syringae pv. tabaci 6605 (Pta6605) bound to the promoter of AHL synthase gene, psyI without AHL, and activated its transcription with AHL. PsyR also bound to the imperfect inverted repeat located in the promoter of hrpL, a gene for transcriptional activator of hrp type III secretion system genes. Pta6605 and P. syringae pv. tomato DC3000 highly expressed noncoding small regulatory RNAs, rsmZ and rsmY at high cell density conditions. Because PtoDC3000 doesn’t produced AHL, importance of Gac/Rsm signaling pathway in QS was suggested.

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  • Analysis of an infection behavior by chemotactic regulation of phytopathogenic bacteria

    Grant number:26660035  2014.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    ICHINOSE Yuki

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    Grant amount:\3900000 ( Direct expense: \3000000 、 Indirect expense:\900000 )

    Plant pathogenic bacterium, Pseudmonas syringae has about 45 genes encoding chemotaxis sensor protein (methyl-accepting chemotaxis protein, MCP). This indicates that P. syringae responds to a variety of chemoattractants. In this study, we revealed that highly motile phytopathogenic bacterium, P. syringae pv. tabaci 6605 (Pta6605) showed positive chemotactic response to 10 compounds including kinetin. P. aeruginosa, a closely related bacterium to P. syringae, has only about 13 mcp genes. Thus we selected 27 mcp genes that are specific to P. syringae and not existed in P. aeruginosa. We generated mutant strains which lost each 27 mcp gene in Pta6605. Although most of the mcp mutants respond to kinetin as well as WT strains, only mutants for mcp4, mcp15b and mcp24 reduced the responsibility. These results indicate that these MCPs seem to be sensor proteins required for kinetin perception.

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  • Elucidation of global network of regulatory mechanism in virulence-related gene expression of phytopathogenic bacteria

    Grant number:24380028  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ICHINOSE YUKI

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    Grant amount:\19240000 ( Direct expense: \14800000 、 Indirect expense:\4440000 )

    Motility in Pseudomonas syringae requires flagella and type IV pili (T4P). Flagella and T4P are required for production of acyl homoserine lactones (AHL), quorum sensing molecules and cAMP, respectively. Production of AHL and cAMP also requires transcription factors AefR and PsyR and Vfr, respectively. Defect of aefR or psyR resulted in the enhancement of multidrug efflux pump genes mexEF/oprN, whereas defect of vfr resulted in the enhancement of another pump genes mexAB/oprM. Enhancement of both pump genes activated multidrug tolerance. PsyR and Vfr are also involved in the regulation of hrp gene expression.

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  • Induction of Hypersensitive Response and Susceptibility by Protease Effector from Ralstonia solanacearum

    Grant number:24658042  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    ICHINOSE Yuki, MUKAIHARA Takafumi

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    Ralstonia solanacearum causes bacterial wilt disease in more than 200 plant species, including economically important Solanaceae species. R. solanacearum injects virulence factor proteins effectors into plant cytoplasm, then induces hypersensitive response (HR) in nonhost plants and disease in host plants. In this study we identified one effector Rip36 induces HR in the nonhost wild eggplant Solanum torvum in an Hrp-dependent manner. Rip36-induced HR requires the putative Zn-protease motif. On the other hand, Rip36 is a dispensable virulence factor to cause disease in eggplant, because rip36-defective mutants grew well in host eggplants as the wild type.

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  • Comprehensive analysis of receptor-like kinase involved in MAMP recognition in Arabidopsis thaliana

    Grant number:21380030  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ICHINOSE Yuki, INAGAKI Yoshishige

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    Grant amount:\18850000 ( Direct expense: \14500000 、 Indirect expense:\4350000 )

    Bacterial non-methylated DNA was identified as one of microbe/pathogen-associated molecular patterns(MAMP/PAMP). We investigated harpin recognition system in tobacco plant as one of molecular patterns and found that an ancestor of Pseudomonas syringae pv. tabaci might have disrupted harpin gene, hrpZ by an internal deletion to evade tobacco defenses and confer the ability to cause disease in tobacco plants. Although we screened the receptor genes for bacterial DNA and/or harpin protein in Arabidopsis thaliana, the corresponding mutant lines were not identified.

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  • 植物病原細菌の病原性糖タンパク質糖鎖の構造解析と病害防除への利用

    2007 - 2011

    新技術・新分野創出のための基礎研究推進事業 

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    Grant type:Competitive

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  • 植物の葉における斑入りと環境適応

    Grant number:19657017  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Exploratory Research  Grant-in-Aid for Exploratory Research

    坂本 亘, 一瀬 勇規

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    本研究では、植物における斑入り突然変異が個体レベルでの光合成としては負に働くか、環境適応に有利に働く場合があることを実証する実験をモデル植物で試みた。特に、環境適応の1つとして斑入りが「病害抵抗性」を示す可能性について萌芽的研究を行った。これまでの研究で、代表者らが解析を進めているシロイヌナズナの斑入り変異var2では光化学系IIの維持に必要な葉緑体プロテアーゼが欠損しており、その結果として葉緑体に高レベルの活性酸素を蓄積することを明らかにしている。昨年度は、これらの活性酸素が病原細菌への抵抗性に関与するかどうかをP. syringae pv tomato DC3000を用いて調べ、野生型に比べ、病原細菌の増殖が抑制されることが明らかとなった。今年度は、これらの抵抗性の原因として斑入り変異では自然免疫の活性化が起きている可能性、葉緑体の活性酸素自身がDC3000への抵抗性に寄与する可能性について調べた。自然免疫活性化の指標となるPR1遣伝子の発現上昇がvar2の葉で観察されず、またこれらの活性化に関わるシグナル因子であるサリチル酸の上昇も見られなかった。他の遺伝子発現の変化もマイクロアレイ等で解析したところ、やはり自然免疫活性化に関する遺伝子発現上昇を支持する結果は得られなかったが、var2の斑入り葉では活性酸素消去系に関わる遺伝子発現が上昇していることが明らかとなった。特に斑入りの白色組織でSODなどの消去系酵素が働いており、抵抗性に寄与する可能性も示唆された。一方で、斑入りを示さない光化学系II酸素発生系複合体に関する突然変異体でも活性酸素が蓄積し、var2よりは弱いがDC3000に抵抗性を示す結果が得られたことから、葉緑体の活性酸素が自然免疫系を活性化するよりもむしろ、葉緑体で蓄積する活性酸素が直接病原細菌に毒性を示す可能性が示唆された。

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  • Mechanisms for the activation and evasion of plant immunity by flagellin from phytopathogenic bacteria

    Grant number:18380035  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ICHINOSE Yuki

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    Grant amount:\17920000 ( Direct expense: \15100000 、 Indirect expense:\2820000 )

    植物病原細菌Pseudomonas syringae pv. tabaci(Pta)の鞭毛構成タンパク質フラジェリンは非宿主植物に対し激しい防御応答(HR)を誘導するが、宿主タバコには顕著には誘導しない。本研究ではフラジェリン分子中のHR誘導ドメインがアミノ末端側の保存配列(flg22)に存在することを見出した。また、フラジェリンに結合するラムノースや修飾ビオサミンなどの糖鎖は、鞭毛の安定性を高めた。従って、糖鎖にはflg22の露出を防ぐことで防御応答を回避する機能が推測された

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  • Mechanism in recognition of non-self sustained by the interaction between organelles in plant cell

    Grant number:15108001  2003 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)  Grant-in-Aid for Scientific Research (S)

    SHIRAISHI Tomonori, ICHINOSE Yuki, INAGAKI Yoshishige, TOYODA Kazuhiro

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    Grant amount:\109720000 ( Direct expense: \84400000 、 Indirect expense:\25320000 )

    In this research, we attempted to clarify the plant defense mechanism in recognition of MAMPs, signal transmission and action of virulence effectors, mediated by the interaction between respective organelles. In this fiscal year, we showed that several molecules interacted with apyrase in plant cell wall by PAGE and TOF/MS/MS analyses. We also presented that MAMPs activated the cell-wall-bound apyrase, resulting in increasing inorganic phosphate that stimulated generation of active oxygen species and expression of defense-related genes. MAMPs also regulated the expression of NbCdc27B, which is a component of M phase in cell cycle. NbCdc27B participated in defense reaction without hypersensitive cell death Microarray analysis indicated that the transcriptional activation of WRKY41 was induced by MAMPs. Transformants highly expressed WRKY41 showed resistance to Pseudomonas syringae, but, inversely, susceptiblity to Erwinia carotovara, indicating that WRKY41 participated in SA signaling pathway. Transportation of proteins via endoplasmic reticulum may be also crucial to defense. Based on these findings, we discuss defense signaling sustained by respective organelles.

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  • 植物の罹病時特異的発現遺伝子の制御機構の解析とその利用に関わる基礎研究

    Grant number:14656019  2002 - 2003

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Exploratory Research  Grant-in-Aid for Exploratory Research

    一瀬 勇規, 稲垣 善茂

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    Grant amount:\3200000 ( Direct expense: \3200000 )

    エンドウ褐紋病菌の生産するサプレッサーは、エリシターによって誘導されるエンドウの防御応答を特異的に抑制あるいは遅延させる。本病原性因子サプレッサーの親和性確立における機能を解析するために、エンドウにおけるサプレッサー応答性遺伝子のcDNAの単離を行い、クローンS64が既に同定されている。S64の遺伝子産物はジャスモン酸合成経路の12-oxophytodienoic acid reductase(OPR)と高い相同性を示す。そこでジャスモン酸合成系の他の酵素であるallene oxide synthase(AOS)とallene oxide cyclase(AOC)をコードする遺伝子のcDNAを単離し、ノーザン法、RT-PCR法によりエリシター・サプレッサー応答、病原菌・非病原菌に対する応答を解析した。その結果、AOSの遺伝子発現はサプレッサー処理や病原菌接種により誘導されることが明らかとなった。一方、S64の遺伝子を単離するためにエンドウゲノムDNAライブラリーよりハイブリダイゼーションによりスクリーニングを行うと、S64以外にOPRと推定される遺伝子が計5個単離され、OPRは遺伝子ファミリーを形成していることが明らかにされた。更に対応するcDNAを単離する試みにおいて新たに6個目のOPR cDNAを同定した。合計6種のエンドウOPR遺伝子について、個々の発現解析を行うとともに、個々の組換えタンパク質を生産し、モデル基質である2-cyclohexen-1-one(CyHE)に対する還元活性を解析した。その結果、S64に相当するPsOPR2遺伝子の発現が最も強くサプレッサー処理・病原菌接種に応答し、その組換えタンパク質が最も高いCyHE還元活性を示した。これらのことより6種のエンドウOPRはそれぞれ異なる基質・発現特性を有していると推測された。現在PsORP2の高発現タバコ、シロイヌナズナ並びにPsOPR2のプロモーターをレポーター遺伝子に繋いだ形質転換植物体を作出している。

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  • Molecular Mechanisms for Regulating Programmed Cell Death during Host-Pamsite Interactions

    Grant number:12052215  2000 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas  Grant-in-Aid for Scientific Research on Priority Areas

    MAYAMA Shigeyuki, PARK Pyon Yuri, ICHINOSE Yuuki, AKIMITSU Kazuya

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    Grant amount:\68200000 ( Direct expense: \68200000 )

    1. Molecular dissection of plant apoptosis using an oat cell free system
    The host specific toxin, victorin induces apoptotic cell death in sensitive oat cultivars. We have developed an oat cell-free apoptosis system to investigate the execution mechanisms of plant apoptosis. Cell extracts derived from oat tissues undergoing victorin-induced apoptosis caused nuclear collapse and internucleosomal DNA fragmentation in isolated nuclei Pharmacological studies revealed that cysteine pretense, which is E-64-sensitive but insensitive to caspase-specific inhibitors, is a crucial component in the morphological change of isolated nuclei, and that nuclease and the cysteine protease act cooperatively to induce the apoptotic DNA laddering. Interestingly, this finding is contrasted with those in well-studied animal cell-free systems in which an apoptotic endonuclease is solely responsible for the DNA fragmentation.
    2. Molecular mechanisms of induction and suppression of plant hypersensitive response triggered by proteinaccous elicitors INF] elicitin from Phytophthora infestans, harpin and flagellin from Pseudomonas syringae induce hypersensitive cell death. Pharmacological investigation of the signal transduction pathway leading to hypersensitive response (HR) revealed that HR requires de novo protein synthesis and protein phosphorylation. Flagellin is a newly identified HR elicitor, and its HR activity is regulated by post-translational glicosyl-modification. We also identified novel defense response-related plant genes, and characterized their structure, expression and function. One of the interesting plant genes, OPR is found to be induced specifically in compatible interactions. OPR may be involved in the suppression of plant defense response.
    3. The mode of action of the host specific toxin, ACR toxin
    Specificity in the interaction between rough lemon and the fungal pathogen Alternaria alternate rough lemon pathotype is determined by a host-selective toxin, ACR-toxin. The target site of ACR-toxin is mitochondrion, and mitochondrial DNA sequence, designated ACRS (ACR-toxin Sensitivity gene), is responsible for the toxin sensitivity. We identified that sensitivity/or insensitivity to ACR-toxin and hence specificity of the interaction between the A.alternata rough lemon pathotype and host plants is due to differential post-transcriptional processing of ACRS.

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  • Analysis of the transcriptional activators for plant defense response-related genes

    Grant number:12460023  2000 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ICHINOSE Yuki, TOYODA Kazuhiro

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    Grant amount:\13200000 ( Direct expense: \13200000 )

    In this study, we investigated the structure and function of novel transcription factor E84 (ERDP) that is isolated from elicitor-treated pea epicotyls. E84 is a plant specific transcription factor possessing Dof-type DNA binding domain at the N-terminus. Random site selection assay for E84 revealed that the recombinant E84 protein binds to AAAG-containing DNA fragments. DNA binding assay using a model DNA fragment, BS4/37 (TCATTTAAAGTGTTTT) that could bind to E84, revealed that not only AAAG core sequence but also 3'-TGT sequence were required for high affinity of the binding. E84 protein also bound to the DNA fragments in the promoter of the elicitor-responsive gene, PsCHS1, which contain AAAG sequence. When we carried out transient transfection assay with pea protoplasts and reporter plasmid possessing 4 repeats of "BS4/37"/CaMV 35S minimal promoter plus reporter gene, we observed that the elicitor enhanced reporter gene activity, indicating the possible involvement of AAAG sequence in elicitor-mediated transcriptional activation. We also observed that E84 protein binds to the promoter sequence of E84 gene itself, indicating possible mechanism of auto-regulation. These results show that E84 protein and its binding sequences might contribute to the elicitor-mediated transcriptional regulation. Furthermore, we also found that at least 7 members of the Dof gene family in pea. These observations provide the information to elucidate the mechanism of the transcriptional activation for plant defense-response related genes.

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  • Analysis of host genes that expression was regulated by the signal molecules produced by phytopathogen

    Grant number:09660051  1997 - 1999

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    ICHINOSE Yuki, SHIRAISHI Tomonori

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    Grant amount:\3300000 ( Direct expense: \3300000 )

    Pea blight pathogen, Mycosphaerella pinodes secrets two kinds of signal molecules, elicitors and suppressors in a germination fluid. Elicitors induce defense response non-specifically on plants and suppressors negate the induced defense response on only host plant. In this study, we have promoted to isolate cDNA clones that are induced by elicitor-treatment to totally understand the defense response and by suppressor-treatment to characterize the mechanisms for the establishment of the compatibility by differential screenings.
    As elicitor-inducible genes, we have first constructed a cDNA library from polyA+RNA prepared from elicitor-treated pea epicotyls for 5hr. By the differential screening, we have isolated 90 cDNA clones as candidates for elicitor-responsive genes. Sequencing analysis of these clones revealed as follows. 1) M. pinodes elicitor might induce gene expressions of hydroxyproline-rich glycoprotein and peroxidase to strengthen plant cell wall. 2) Putative cDNAs for polygalacturonase inhibiting protein and endochitinase demonstrates that these genes might be effective in incompatible interactions. 3) Isolation of many cDNA clones encoding the enzymes for polyamine biosynthesis indicates the importance of polyamine in defense response. 4) A cDNA clone encoding novel Dof type zinc-finger transcription factor binds specially to AAAG sequence present in the promoter region of many defense-related genes. These results indicate that the significance of this protein in the regulation of complex network of transcriptional regulation by the elicitor-mediated activation of a number of defense response-related genes.
    On the other hands, as suppressor-responsive genes, cDNA clones encoding homologs of early nodulin, cell wall degrading enzymes and biosynthetic enzyme for jasmonic acid were isolated. Particularly, corresponding mRNA to jasmonic acid biosynthetic enzyme was specifically induced by the inoculation of compatible pathogen in pea leaves. The investigation of these susceptible specific genes might reveal the mechanism of the establishment of compatible interactions.

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  • Molecular breeding of transgenic solanaceous plants showing the resistance to Ralstonia solanacearum by the introduction of lysozyme gene

    Grant number:09356002  1997 - 1999

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    YAMADA Tetsuji, ICHINOSE Yuki, TAHARA Makoto, OZAWA Hidenori

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    Grant amount:\17600000 ( Direct expense: \17600000 )

    Ralstonia solanacearum is a causal agent of bacterial wilt mainly to solanacearum plants. Although many approaches were made to control this pathogen, a sure method to protect the plants was not established yet. In this study, to breed genetically resistant solanacearum plants to this pathogen, we planed to generate the transgenic tobacco plants expressing the lysozyme gene isolated from the bacteriophage of this bacterium. Furthermore we utilized pea PSPAL1 promoter which shows root spec and pathogen-inducible expression pattern for the control of lysozyme gene.
    We considered that the following results include the significant achievements in this research.
    1. From bacteriophage P4282 of Ralstonia solanacearum, we isolated lysozyme protein and correspond gene, and structurally characterized.
    2. We constructed the repeat promoter consisting the regulatory cis-elements (Box I, Box, II and Box IV of PSPAL1 promoter that expressed extremely high promoter activity. Especially the repeat promoter exhibited 6.5 hold activity than CaMV 35S promoter in roots.
    3. Each CaMV 35S promoter, PSPAL1 promoter or the repeat promoter described above was fused with lysozyme gene, and transformed tobacco plants.
    4. The transgenic tobacco plants that integrated chimeric lysozyme gene expressed higher lytic enzyme activity.
    5. By the inoculation of 35S :: Lux gene cassette introduced R. solanacearum on the transgenic tobacco plants possessing PSPAL1 promoter :: GUS reporter gene enabled to establish the monitoring system to investigate the host defense response and behavior of bacterial pathogen simultaneously.

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  • ストレス応答遺伝子とアポトーシス関連遺伝子の器官特異的発現と環境シグナル応答

    Grant number:09251211  1997

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas  Grant-in-Aid for Scientific Research on Priority Areas

    山田 哲治, 一ノ瀬 勇規

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    Grant amount:\2000000 ( Direct expense: \2000000 )

    色素生産やストレス応答において、特に重要な役割を担うフェニールプロパイド二次代謝合成系の鍵酵素をコードするPALおよびCHS遺伝子をモデルとして、多重遺伝子ファミリーの各メンバーの植物各器官・組織特異的発現に関わる制御領域を同定するとともに、鍵となる制御タンパク質をコードする遺伝子を単離して、フェニールプロパイド二次代謝経路を支配する遺伝的制御プログラムを分子生物学的に解析すること、また、植物の病原体攻撃に対する植物の典型的な防御機構に、過敏感細胞死(Hypersensitive Cell Death:HR)において物のアポトーシス現象を正および負に調節する遺伝子を探索し、これらの調節因子が、植物の器官形成にどのような役割を果たしているのかを明らかにすることを狙いとしている。
    環境シグナル及び器官特異的発現に関与するシス領域のLM-PCRによる解析
    病原菌エリシター,あるいはサプレッサーによる遺伝子発現誘導応答に必要と考えられる重要なシス因子の候補に関して、PSPAL-1,-2およびPSCHS-1,-2を中心にLM(Ligation mediated)-PCR footprinting解析を行ったところ、エリシター処理によるこれらの遺伝子のプロモータの活性化にはACに富むシ-クネンスボックス(Box l)が関与していることを示す顕著なfootprintが確認された。現在、器官特異的に発現に関与する特異的なfootprintおよびサプレッサー処理による遺伝子発現抑制に関与するfootprint解析を行っている。
    アポトーシス関連遺伝子のクローニング
    タバコBY-2細胞を用いて、Activation taggingを行い、エリシター処理に対して過敏感細胞死を遅延したり、起こさなくなった変異体細胞を得た。これらの細胞からplasmid rescueを行い、タバコ・ゲノミックDNAの挿入を確認したところ、ひとつのカルス集団には約5種類のタバコ・ゲノミックDNAが挿入されていた。そこでDNA断片を再度、組換えプラスミドに挿入し、タバコBY-2細胞に遺伝子挿入したところ、一つのラインでエリシター処理しても細胞死を起こさない変異体が出現した。現在本DNAの塩基配列決定を行っている。

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  • フェニールプロパノイド合成系鍵酵素遺伝子の器官特異的発現と環境シグナル応答

    Grant number:08262215  1996

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas  Grant-in-Aid for Scientific Research on Priority Areas

    山田 哲治, 一瀬 勇規

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    Grant amount:\2500000 ( Direct expense: \2500000 )

    1.遺伝子改変プロモータを用いたLoss of functionの実験結果から、PSPAL1遺伝子5′-プロモータ領域に含まれるエリシターよる発現誘導応答に必要なシス因子として、box 1(L-box)およびbox 2(P-box)があげられる.このことはbox 2とbox4を4-8ユニット繰り返し配列で繋いだキメラ・プロモータを用いたGain function実験からも更に裏付けられた.トランジェントアッセイの解析に加え、box 1(L-box),box 2(P-box),box 4(AT-box)各ボックスシークエンスを除去した遺伝子改変プロモータをGUSレポーター遺伝子に連結したpBI系遺伝子組換えプラスミドをタバコに導入し、トランスジェニックタバコにおけるin planta loss of function実験を行った.その結果、これらのボックスシークエンスは、エリシターやUV照射による発現誘導応答に必要であるばかりでなく、basalなPSPAL1のプロモータの発現にも影響を及ぼすことが明らかとなった.これらのシス因子は単独ではプロモータの活性化に及ぼす効果が少なく、これらのボックスに結合する調節タンパク質が協調的に働いていると考えられる.
    2.エンドウカルコン合成酵素(CHS)遺伝子は少なくとも8つのメンバーからなる遺伝子ファミリーを形成していることが明らかとなり、各々のメンバーの組織特異的・環境刺激特異的発現パターンを調べたところ、CHS遺伝子ファミリーの8つのメンバーは少なくとも3つのサブファミリーにグループ分けすることができることが明らかとなった。これらのうち2つのエリシター応答性グループのプロモータにはBox-1,G-Box,Box-2配列が保存されており、これらの配列がエリシター応答に重要であることが、塩基置換を導入したプロモータのトランジェント・トランスフェクション・アッセイによっても確認された。また、エリシター等のシグナルによる急激なCHS活性の増加は転写の活性化のみならず、遺伝子ファミリーの各メンバーの協調的な活性化、並びに転写後の調節機構にも基因することが推察された。

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  • フェニールプロパノイド合成系鍵酵素遺伝子の器官特異的発現と環境シグナル応答

    Grant number:07270216  1995

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas  Grant-in-Aid for Scientific Research on Priority Areas

    山田 哲治, 一瀬 勇規

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    Grant amount:\2700000 ( Direct expense: \2700000 )

    植物のフェニールプロパノイド二次代謝経路はアントシアニン色素、リグニンを中心とする細胞外皮の構築、紫外線毒の吸収、あるいは病原菌の攻撃に対するファイトアレキシン合成など、多岐にわたる機能を果たしている。エンドウ・フェニールプロパノイド合成系の重要な鍵酵素、フェニールアラニンアンモニア・リアーゼ(PAL)およびカルコン合成酵素(CHS)をコードする遺伝子は多重遺伝子ファミリーを形成しているが、それぞれのメンバーの植物各器官および組織特異的発現、あるいは外的環境要因による遺伝子発現調節機構に関しては未知の部分が多い。本研究では、色素生産やストレス応答において特に重要な役割を担うフェニールプロパノイド二次代謝合成系の鍵酵素をコードするPAL遺伝子、およびCHS遺伝子をモデルとして、多重遺伝子ファミリーの各メンバーの植物各器官・組織特異的発現に関わる制御領域を同定するとともに、鍵となる制御タンパク質をコードする遺伝子を単離して、フェニールプロパノイド二次代謝経路を支配する遺伝的制御プログラムを分子生物学的に解析することを目的としている。以下に成果の概要を列記する。
    1. 遺伝子改変プロモータを用いたLoss of functionの実験結果から、PSPAL1遺伝子5′-プロモータ領域に含まれるエリシターによる発現誘導応答に必要なシス因子として、box1 (L-box) ,box2 (P-box) ,box4 (AT-box)があげられた。このことはbox2とbox4を2-4ユニット繰り返し配列で繋いだキメラ・プロモータを用いたGain of function実験からも更に裏付けられた。これらのシス因子は単独ではプロモータ活性化に及ぼす効果が少なく、協調的に働いていると予想された.
    2. 上記トランジエントアッセイの解析に加え、box1 (L-box) ,box2 (P-box) ,box4 (AT-box)各ボックスシークエンスを除去した遺伝子改変プロモータをGUSレポータ遺伝子に連結したpBI系遺伝子組換えプラスミドをタバコに導入し、トランスジェニックタバコにおけるin planta loss of function実験を行った.その結果、これらのボックスシークエンスは、エリシターやUV照射による発現誘導応答に必要であるばかりでなく、basalなPSPAL1,PSPAL2プロモータの発現にも影響を及ぼすことが明らかとなった.現在、これらのボックスシークエンスの器官・組織特異的発現に果たす役割を検討している。

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  • Studies on molecular mechanisms in the signal transduction cascade from signal perception to the expression of defense responsive genes in a plant-pathogen interaction

    Grant number:06404008  1994 - 1996

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    SHIRAISHI Tomonori, ICHINOSE Yuki, YAMADA Tetsuji

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    Grant amount:\29300000 ( Direct expense: \29300000 )

    In this study, we attempted to clarify the molecular mechanisms in the signal transduction cascade between perception of fungal signal molecules and the expression of defense responsive genes.
    Our findings are summarized as follows :
    1. ATPase and polyphosphoinositide metabolism in pea plasma membranes are regulated by the clicitor and suppressor from Mycosphaerella pinodes. The suppressor, however, blocked nonspecifically them even in plasma membranes isolated from non-host plants of the fungus.
    2. The M.pinodes-elcitor enhanced nonspecifically the activities of ATPase (NTPase), peroxidase and ascorbic acid oxidase in NaCl-soluble fraction from cell walls isolated from pea and cowpea, whereas the suppressor blocked these activities in a species-specific manner.
    3. Superoxide anion and as yet unidentified infection-inhibitor, that were generated in isolated pea cell wall by the elicitor, were inhibited by the suppressor. On the other hand, those in cowpea cell wall were inversely elicited by the suppressor alone.
    4. Signal transduction leading to phytoalexin production was mediated by connection of certain vitronectin-like molecules in cell wall and vitronectin receptor-like molecules on plasma membrane.
    5. In the promoter sequence of PSPALs and PSCHSs, we found conserved sequence motifs, such as Box-1 (homologous to Box L), Box-2 (homologous to Box P) and Box-4 (AT-box) that were essential for response to the elicitor.
    6. In PSCHSL the directly repeated AT-rich sequences, TAAAATACT were capable of forming a low mobility complex (LMC) by a gel mobility shift assay and in vitro DNase i-footprinting analysis. The finding, that treatment of the nuclear factors with alkaline phosphatase abolished LMC formation, indicates phosphorylation of nuclear protein (s) is necessary for the formaiton of complex machinery.
    7. The cis-elements such as Box1, Box2 and G-box were essential for the promoter of elicitor-responsive CHS genes.
    From these results, we propose the stream of signal transduction leading to defense responses as follows : 1) signal molecules are percepted by respective receptors on cell wall ; 2) the second messengers are carried out into the cells via vitronectin receptor-like proteins on the plasma membranes ; 3) polyphosphoinositide metabolism in plasma membranes is also activated to produce the 3rd messenger ; 4) the messenger stimulates phosphorylation of nuclear factor (s) ; 5) The transcription of defense responsive genes are activated ; 6) the rejection reaction is expressed in the clicitor-treated plant tissues.

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  • Study of the defense response and gene regulation for pathogen attack

    Grant number:06454063  1994 - 1995

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (B)  Grant-in-Aid for General Scientific Research (B)

    YAMADA Tetsuji, ICHINOSE Yuki

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    Grant amount:\7200000 ( Direct expense: \7200000 )

    The object of this research is to elucidate the mechanisms of signal transduction and control of the genes involved in plant active defense responses conferring host-pathogen specificity. We intensively characterized the molecular basis involved in the activation and deactivation of plant active defense genes encoding PAL and CHS in pea, particularly focused on the study of the effects of elicitor and suppressor produced by a pea pathogen, Mycosphaerella pinodes. Details of the current status of the studies are,
    1.Artificial reconstruction of a members of PAL gene (PSPAL1) and in a small multigene family of pea demonstrated that the conserved sequence motifs of Box 2 and Box 4 were very important in the activation mediated by the treatment with fungal elicitor by so called Loss of Function and Gain of Function Experiments. However, Box 1 functions in a concerted manner with Box 2 and Box 4, probably forming a transcriptional activation complex.
    2.Generation of transgenic tobacco carrying the chimeric PSPAL1 promoter-GUS-Nos terminator construct (same types of the chimeric gene as described in 1 except these construct are connected to GUS reporter gene instead of CAT gene) demonstrated that Boxes1,2, and 4, are prerequisite cis-elements in response to the pathogen attack. These elements are also important in response to the activation mediated by the treatment with fungal elicitor. -1,394 to +140 or to +115 of PSPAL1 (translational fusion type) promoter contributed the tissue-specific expression, particularly in the merismatic tissues of roots, lower part of stems and very high expression in the tissues of flower organs where pink pigment is deposited and lignins are vigorously for med. Chimeric fusion of -1,394 to +5 or to -27 exhibited very little GUS expression, indicating that the exonic sequences are important in the expression if transgenic tobacco.

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  • エンドウカルコン合成酵素遺伝子の病原菌シグナルによる発現制御機構

    Grant number:06760050  1994

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Encouragement of Young Scientists (A)  Grant-in-Aid for Encouragement of Young Scientists (A)

    一瀬 勇規

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    Grant amount:\1000000 ( Direct expense: \1000000 )

    エリシターによりエンドウCHS遺伝子の転写誘導に必要なDNAシス領域を同定するために、まず、様々な長さのPSCHS1、PSCHS2遺伝子5´上流域をレポーター遺伝子であるクロラムフェニコールアセチルトランスフェラーゼ(CAT)遺伝子に結合させてキメラ遺伝子を構築した。次に、これらのキメラ遺伝子をエンドウ培養細胞由来のプロトプラストにエレクトロポレーション法で導入して、一時的なCATの活性を測定した。この時エレクトロポレーション後にエリシター処理を行う区と行わない区を設けてエリシター処理による影響も解析した。その結果、PSCHS1では少なくとも-242から下流に、PSCHS2では-377から下流にエリシター応答性転写活性領域が存在することが明らかとなった。更にCaMVの35Sミニマルプロモーターを用いたgain of function実験からPSCHS1の-242から-181までの61塩基だけでエリシターに応答してレポーター遺伝子の発現を増高させることが明らかになった。次に、両遺伝子の転写制御に関わる核内タンパク質因子について、ゲルシフトアッセイ法により解析した。その結果、PSCHS1の-242から-181の61塩基の断片やPSCHS2の-347から-158までの189塩基の断片はエリシター処理エンドウ組織から調製された核タンパク質と強く結合して移動度の遅い複合体(LMC)を形成することが明らかとなった。LMCの形成は核タンパク質を予めアルカリフォスファターゼで前処理することによって阻害されたことから、LMCの形成にはリン酸化が必要であると考えられる。また、エリシターで転写が誘導されるエンドウのPSPAL1とPSPAL2のプロモーターにも同様なLMCを形成する領域が存在しており、それぞれのLMCの形成は互いにLMCを形成するDNA断片により競合することが明らかにされた。以上の結果から、エリシターによる防御遺伝子の転写誘導にはLMCを形成する共通因子が関与している可能性が示唆された。

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  • フェニールプロパノイド合成系鍵酵素遺伝子の器官特異的発現と環境シグナル応答

    Grant number:06278214  1994

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas  Grant-in-Aid for Scientific Research on Priority Areas

    山田 哲治, 一瀬 勇規

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    Grant amount:\2400000 ( Direct expense: \2400000 )

    植物のフェニールプロパノイド二次代謝経路はアントシアニン色素、リグニンを中心とする細胞外皮の構築、紫外線毒の吸収、あるいは病原菌の攻撃に対するファイトアレキシン合成など、多岐にわたる機能を果たしている。エンドウ・フェニールプロパノイド合成系の重要な鍵酵素、フェニールアラニンアンモニア・リアーゼ(PAL)およびカルコン合成酵素(CHS)をコードする遺伝子は多重遺伝子ファミリーを形成しているが、それぞれのメンバーの植物各器官および組織特異的発現、あるいは外的環境要因による遺伝子発現調節機構に関しては未知の部分が多い。本研究では、色素生産やストレス応答において特に重要な役割を担うフェニールプロパノイド二次代謝合成系の鍵酵素をコードするPAL遺伝子、およびCHS遺伝子をモデルとして、多重遺伝子ファミリーの各メンバーの植物各器官・組織特異的発現に関わる制御領域を同定するとともに、鍵となる制御タンパク質をコードする遺伝子を単離して、フェニールプロパノイド二次代謝経路を支配する遺伝的制御プログラムを分子生物学的に解析することを目的としている。以下に成果の概要を列記する。
    1.Loss of function実験からPSPAL1,PSPAL2各遺伝子5′-プロモータ領域に含まれるエリシターあるいはUV照射による発現誘導応答に必要なシス因子の候補としてbox1(L-box),box2(P-box),box4(AT-box)があげられた。このことはゲル・モビリテイー・シフトアッセイやDNase I-,OP-Cu footprinting analysisによっても確かめられた。
    2.トランスジェニックタバコにおけるキメラ遺伝子の発現様式からPSPAL1,PSPAL2プロモータはともに、根.下位茎における組織特異的発現に関与していることが示唆された。また、PSPAL1プロモータは、さらに花弁の色素沈着部における特異的発現に関与していることが明らかとなった。
    3.PSPAL1,2、およびPSCHS1,2のすべてのプロモータ領域のATリッチシークエンスに結合する共通のタンパク質はHMG-I/Yに類似するDNAベンデイングに関与するタンパク質である可能性が大きい。

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  • Regulation of the plant defense gene expression

    Grant number:03044100  1991

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for international Scientific Research  Grant-in-Aid for international Scientific Research

    YAMADA Tetsuji, ICHINOSE Yuki, SHIRAISHI Tomonori, OKU Hachiro, KADO Clarence I.

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    Grant amount:\3000000 ( Direct expense: \3000000 )

    The organization and the structure of the genes encoding phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) were determined and the effects of elicitor and suppressor produced by a pea pathogen, Mycosphaerella pinodes, were examined by transforming a chimeric DNA containing the PAL- and CHS-promoter fragments connected to a reporter gene into tobacco or pea plant with particle gun, Agrobacterium-mediated transformation, or electroporation.
    1. Analysis of PAL genes. Chimeric DNA containing PAL promoter fragment connected to luciferase gene (lux), beta-glucuronidase (GUS) or chloramphenicol acetyltransferase (CAT) gene as a reporter were constructed. Chimeric DNA (PAL-GUS) in the vector, pBI101, were introduced into tobacco and pea by Agrobacterium mediated- transformation. Km^R-transgenic tobacco plants were regenerated from the callus. GUS activities were induced by the treatment with pathogen or elicitors in some of the transgenic plants. Since the regeneration line of pea plant has not been established from the single cell lines, we obtained Km^R calli and the induction of GUS activities were examined by the treatment with fungal elicitor and the infection of pathogens. Considerable induction of GUS activity was observed in some of the callus. The integration of the chimeric DNA into the plant chromosomes are currently under investigation. Gene transfer by using a particle gun (Dupont Inc.) into these plants were performed at UCD, Davis. Cytological studies showed that plants with Lux or GUS gene connected to 35S promoter as a control exhibited the distinctive GUS atibity in the cells possibly carrying the chimeric DNA, however those with PAL promoter did not exhibit GUS or Lux activities even by the treatment with fungal elicitor. Furthermore, the sequentially deleted promoter fragment of PSPAL1 connected to a plant expression vector containing the reporter gene (CAT) was introduced into pea protoplasts by electroporation, and induction by elicitor and suppression by orthovanadate was examined. Cis-element responsible for these regulations exists between -340--140 of PSPAL1.
    2. Analysis of CHS gene. The structure and the organization of three major CHS genes were determined from 38 CHS-cDNAs prepared from elicitor treated pea epicotyl tissues. Two of the major pea CHS genes (PSCHS1, PSCHS2) from a cluster in a tandem repeat with a spacer, and an identical 31 bp sequence containing the consensus motif of box I located 5'-upstream from the putative TATA box of both CHS genes. Buth were highly expressed even without the treatment with fungal elicitor by transient transformation assay mentioned above, which indicate that the activator elements exist in the promoter-distal region (PCHS1 : -1493 - +80, and PCHS2 : - 1889 - +83).
    3. Isolation of signalling substances from the pathogen and study of the signal transduction pathway Prof. C. I. Kado visited our laboratory on December, 1991, and discussed about the signaling substances produced by the pathogens. We particularly focused on the substances that blocked the induction of defense reactions. Since M. pinodes suppressor specifically inhibits pea plasma membrane ATPase, he examined bacterial signalling molecules from the aspect of blocking PM-ATPase.

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  • Host-pathogen specificity and a control of the expression of active resistant genes

    Grant number:02404012  1990 - 1991

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (A)  Grant-in-Aid for General Scientific Research (A)

    OKU Hachiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

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    Grant amount:\19100000 ( Direct expense: \19100000 )

    Effects of elicitor and suppressor produced by a pea pathogen, Mycosphaerelia pinodes, were examined on the signal transduction and the regulation of the genes encoding PAL and CHS in pea active defense responses.
    1. Mode of action on plasma membrane (PM) ATPase. Suppressor inhibits PM-ATPase not only from pea but also from other plant species tested in in vitro assay, but cytological studies showed that it specifically inhabits only pea PM-ATPase. Moreover, orthovanadate, a non-specific inhibitor for PMATPase, functions in delaying the induction of the expression of PAL and CHS in a manner very similar to a fungal suppressor.
    2. Effects on PM phosphoinisitides (PI) metabolisms. The phosphorylation of PIP and PIP2 by lipid kinase was stimulated by the treatment with elicitor, but it was distinctively suppressed in the concomitant presence of suppressor.
    3. Structure of PAL genes and the control of the gene expression. Structure and gene organization of the two members of PAL gene (PSPALI and PSPAL2) in a small multigene family were determined, and the accumulation of the transcripts of each member in the tissues was examined using sequence specific probe. High level of the accumulation of both transcripts'was observed in roots and the lower portion of the stems, but very little in other organs, which suggests that PSPALI and PSPAL2 may contain ciselement responsible for tissue specific expression particularly in roots. Furthermore, the sequentially deleted promoter fragment of PSPALI connected to a plant expression vector containing the reporter gene was introduced into pea protoplasts by electroporation, and induction by elicitor and suppression by orthovanadate was examined. Cis- elei-nent responsible for these regulations exists between -340100 of PSPALI.
    4. Structure and organization of CHS gene. Pea CHS genes (PSCHSI, PSCHS2) form a cluster in a tandem repeat with a spacer, and an identical 31 bp sequence containing, the consensus motif of box I locates 5'-upstream from the putative TATA box of both CHS genes.

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