Updated on 2022/09/25

写真a

 
OHARA Naoko
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Associate Professor
Position
Associate Professor
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Degree

  • D.D.S. ( Nagasaki University )

Research Interests

  • dental caries

  • composite resin

  • adhesive dentistry

Research Areas

  • Life Science / Conservative dentistry

Education

  • Nagasaki University    

    - 1996

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  • Nagasaki University   大学院 歯学研究科  

    - 1996

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    Country: Japan

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Research History

  • Okayama University   学術研究院医歯薬学域 歯科保存修復学分野   Associate Professor

    2021.12

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  • Okayama University   大学病院 むしば科   Lecturer

    2017.5 - 2021.11

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  • Okayama University   大学院医歯薬学総合研究科歯科保存修復学分野   Assistant Professor

    2010.4 - 2017.4

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  • -:Assistant Professor, Nihon University School of Dentistry Department of Fixed Prosthodontics

    2007.4 - 2010.3

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  • -:日本大学歯学部歯科補綴学教室Ⅲ講座助教

    2007.4 - 2010.3

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  • :長崎大学大学院医歯薬学総合研究科医療科学専攻展開医療科学講座

    2006 - 2007

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  • :Instructor, Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences

    2006 - 2007

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  • :長崎大学大学院医歯薬学総合研究科医療科学専攻発生分化機能再建学講座齲蝕学分野助手

    2002 - 2006

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  • :Instructor, Division of Cariology, Graduate School of Biomedical Sciences, Nagasaki University School of Dentistry

    2002 - 2006

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  • :長崎大学歯学部歯科保存学第一講座助手

    1997 - 2002

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  • :Instructor, Department of Endodontics and Operative Dentistry, Nagasaki University School of Dentistry

    1997 - 2002

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  • :長崎大学歯学部付属病院医員

    1997

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  • :Clinical Fellow, Department of Endodontics and Operative Dentistry, Nagasaki University School of Dentistry

    1997

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  • 歯科学群齲蝕学分野助手

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Professional Memberships

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Papers

  • Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG. International journal

    Yuki Arimura, Yusuke Minato, Takayuki Wada, Masaaki Nakayama, Ayako Ryumon, Nao Hirata, Chie Nakajima, Yasuhiko Suzuki, Manabu Ato, Kazuo Kobayashi, Naoko Ohara, Seiji Iida, Naoya Ohara

    Microbiology and immunology   66 ( 1 )   10 - 14   2022.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.

    DOI: 10.1111/1348-0421.12944

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  • Assessment of Demineralization Inhibition Effects of Dentin Desensitizers Using Swept-Source Optical Coherence Tomography. Reviewed International journal

    Kumiko Matsuzaki, Yasushi Shimada, Yasuo Shinno, Serina Ono, Kozo Yamaji, Naoko Ohara, Alireza Sadr, Yasunori Sumi, Junji Tagami, Masahiro Yoshiyama

    Materials (Basel, Switzerland)   14 ( 8 )   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    The purpose of this study was to evaluate the mechanism of action and the inhibiting effects of two types of desensitizers against dentin demineralization using pre-demineralized hypersensitivity tooth model in vitro. In this study, we confirmed that a hypersensitivity tooth model from our preliminary experiment could be prepared by immersing dentin discs in an acetic acid-based solution with pH 5.0 for three days. Dentin discs with three days of demineralization were prepared and applied by one of the desensitizers containing calcium fluoro-alumino-silicate glass (Nanoseal, NS) or fluoro-zinc-silicate glass (Caredyne Shield, CS), followed by an additional three days of demineralization. Dentin discs for three days of demineralization (de3) and six days of demineralization (de6) without the desensitizers were also prepared. The dentin discs after the experimental protocol were scanned using swept-source optical coherence tomography (SS-OCT) to image the cross-sectional (2D) view of the samples and evaluate the SS-OCT signal. The signal intensity profiles of SS-OCT from the region of interest of 300, 500, and 700 µm in depth were obtained to calculate the integrated signal intensity and signal attenuation coefficient. The morphological differences and remaining chemical elements of the dentin discs were also analyzed using scanning electron microscopy and energy-dispersive X-ray spectroscopy. SS-OCT images of CS and NS groups showed no obvious differences between the groups. However, SS-OCT signal profiles for both the CS and NS groups showed smaller attenuation coefficients and larger integrated signal intensities than those of the de6 group. Reactional deposits of the desensitizers even after the additional three days of demineralization were observed on the dentin surface in NS group, whereas remnants containing Zn were detected within the dentinal tubules in CS group. Consequently, both CS and NS groups showed inhibition effects against the additional three days of demineralization in this study. Our findings demonstrate that SS-OCT signal analysis can be used to monitor the dentin demineralization and inhibition effects of desensitizers against dentin demineralization in vitro.

    DOI: 10.3390/ma14081876

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  • Construction and characterization of the PGN_0296 mutant of Porphyromonas gingivalis Reviewed

    Abu Saleh Muhammad Shahriar, Shintaro Ono, Masaaki Nakayama, Naoko Ohara, Naoya Ohara

    Journal of Oral Biosciences   62 ( 4 )   322 - 326   2020.12

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.job.2020.09.007

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  • 乾燥が成形修復材料の質量および曲げ強度に及ぼす影響 Reviewed

    澁谷 和彦, 大原 直子, 入江 正郎, 小野 瀬里奈, 松崎 久美子, 松本 卓也, 吉山 昌宏

    日本歯科保存学雑誌   63 ( 2 )   199 - 206   2020.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

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  • Color Matching Evaluation Using the Visual Analog Scale Reviewed

    Serina ONO, Naoko OHARA, Kumiko MATSUZAKI, Kazuhiko SHIBUYA, Akihito YOKOYAMA, Kei TAKAHASHI, Yasuo SHINNO, Kozo YAMAJI, Yasushi SHIMADA, Masahiro YOSHIYAMA

    The Japanese Journal of Conservative Dentistry   63 ( 1 )   30 - 37   2020.2

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    <p> Purpose: Recently, the application of composite resin restorations has expanded. Although chromatic studies of composite resins have progressed, color differences measured by colorimeters and visual color evaluation do not always match. The aim of this study was to establish a method of evaluating the visual color of composite resins by using the visual analog scale (VAS), which evaluates subjective data as a continuous variable, and to examine its validity.</p><p> Method: One structural color composite resin (experimental resin : ECM-001) and three commercial composite resins of shade A3 were used in this study. Class Ⅴ and class Ⅲ cavities were prepared in artificial maxillary central incisors of four shades (A1, A2, A3, and A3.5) and each composite resin was filled and its color matching was evaluated on a black-and-white background using VAS. The correlation between VAS value and visual evaluation was analyzed. The color matching of teeth and composite resin were statistically analyzed using one-way analysis of variance and Tukey's HSD test (p<0.05). Background differences in the same sample were analyzed by the t-test (p<0.05).</p><p> Results: In the Class Ⅴ restoration, ECM-001 showed better color matching than other composite resins. In the Class Ⅲ restoration, ECM-001 showed excellent color matching with a wide range of teeth shades on the white background. The VAS values of the other composite resins were higher on the black background than on the white background. However, the VAS value of ECM-001 on the black background tended to decrease. Because the evaluation of VAS color matching by evaluators showed the same tendency, the VAS value reflects information that can be recognized by visual observation. It is suggested that the evaluation of VAS value correlates with visual evaluation.</p><p> Conclusion: It is suggested that the evaluation of color matching of composite resin restorations using VAS is useful for structural color composite resins, providing a new method of visually evaluating composite resin restorations.</p>

    DOI: 10.11471/shikahozon.63.30

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  • Influence of 10-MDP concentration on the adhesion and physical properties of self-adhesive resin cements. International journal

    Kazuhiko Shibuya, Naoko Ohara, Serina Ono, Kumiko Matsuzaki, Masahiro Yoshiyama

    Restorative dentistry & endodontics   44 ( 4 )   e45   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    Objectives: Self-adhesive resin cements contain functional monomers that enable them to adhere to the tooth structure without a separate adhesive or etchant. One of the most stable functional monomers used for chemical bonding to calcium in hydroxyapatite is 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP). The aim of this study was to evaluate the influence of the10-MDP concentration on the bond strength and physical properties of self-adhesive resin cements. Materials and Methods: We used experimental resin cements containing 3 different concentrations of 10-MDP: 3.3 wt% (RC1), 6.6 wt% (RC2), or 9.9 wt% (RC3). The micro-tensile bond strength of each resin cement to dentin and a hybrid resin block (Estenia C&B, Kuraray Noritake Dental) was measured, and the fractured surface morphology was analyzed. Further, the flexural strength of the resin cements was measured using the three-point bending test. The water sorption and solubility of the cements following 30 days of immersion in water were measured. Results: The bond strength of RC2 was significantly higher than that of RC1. There was no significant difference between the bond strength of RC2 and that of RC3. The water sorption of RC3 was higher than that of any other cement. There were no significant differences in the three-point bending strength or water solubility among all three types of cements. Conclusions: Within the limitations of this study, it is suggested that 6.6 wt% 10-MDP showed superior properties than 3.3 wt% or 9.9 wt% 10-MDP in self-adhesive resin cement.

    DOI: 10.5395/rde.2019.44.e45

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  • 透明根管模型歯における根管充填の評価法に関する検討 Reviewed

    松崎 久美子, 大原 直子, 澁谷 和彦, 横山 章人, 岡田 俊輔, 村上 純, 久富 美紀, 浅海 淳一, 吉山 昌宏

    岡山歯学会雑誌   38 ( 1 )   11 - 18   2019.6

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    Language:Japanese   Publisher:岡山歯学会  

    歯学部学生の臨床前基礎実習において透明根管模型歯は、全ての学生が同一条件下で実習を行えることや、根管治療における各ステップを目視できる等の利点がある。今回、本学歯学部4年次生47名が象牙質歯髄複合体診断・治療学実習の実技試験で根管充填を行った透明根管模型歯について、視診による評価と、デジタルX線画像のモニタ上での視診による評価、およびデジタルX線画像を画像解析ソフト(ImageJ)により解析した評価を行い、各評価法の妥当性について比較検討した。その結果、画像解析ソフトで解析する方法は成績の過小評価を防ぐうえで有用であり、より客観的な評価が可能と考えられた。

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  • Effects of direct pulp capping on hard tissue formation by using alginate gel containing bone morphogenetic protein-2 Reviewed

    Journal of Oral Tissue Engineering   17   53 - 58   2019

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  • Effects of transforming growth factor-β1 and Bone morphogenetic protein-2 on bone formation using a bioabsorbable scaffold at palatal sites in rats Reviewed

    Yamaji K, Yokoyama A, Shimada Y, Sugaya T, Ohara N, Matsuzaki K, Nishiyama E, Yoshiyama M

    Journal of Oral Tissue Engineering   16   74 - 79   2018

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  • Influence of Dentin Desensitizer on Bond Strength of Self-etching Adhesive System

    Kumiko MATSUZAKI-TANAKA, Naoko OHARA, Kazuhiko SHIBUYA, Serina ONO, Akihito YOKOYAMA, Kozo YAMAJI, Masahiro YOSHIYAMA

    The Japanese Journal of Conservative Dentistry   60 ( 1 )   22 - 31   2017

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    <p> Purpose: Clinically, resin composite repair for dentin hypersensitivity with substantial defects such as wedge-shaped defects is commonly performed a few days after applying the densensitizer to dentin. In this study, we investigated the influence of pre-treatment of dentin densensitizers on bond strength.</p><p> Materials and Methods: We applied three desensitizers (MS coat ONE, F Varnish, and Nanoseal) on an artificial model of hypersensitive dentin using human premolars, and observed the surface structure by scanning electron microscope (SEM). Then, the specimens to which the three desensitizers had been applied were treated with Clearfil Mega Bond FA. We morphologically observed by SEM the surface of dentin which had been treated with the primer. Furthermore, the micro-tensile bond strength (μTBS) for the specimens which were incrementally built up by resin composites was measured in a universal testing machine. The interface between resin and dentin of specimens was observed by SEM and confocal laser scanning microscope (CLSM).</p><p> Results: The surfaces of specimens to which each desensitizer had been applied showed morphological characters having the effects of suppressing hypersensitivity. Regarding μTBS, there were no significant differences among all groups. In the group of MS coat ONE, MS coat ONE was not completely removed by the application of Mega Bond FA primer. Although the penetrations of the primer into dentin tubules were small, the thickness of the bond layer was greater. The specimens of F Varnish showed the functions of self-etching adhesive systems, because the varnishes had been partially removed by Mega Bond FA primer. In the group of Nanoseal, Mega Bond FA primer was almost removed by Nanoseal, and penetrated deeply into the dentinal tubules.</p><p> Conclusion: The three desensitizers used in this study had no effect on the bond strength of self-etching adhesive systems to the artificial model of hypersensitive dentin.</p>

    DOI: 10.11471/shikahozon.60.22

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  • Study of Pluripotency and Activation of Totipotency in Human Adipose-derived Stem Cells

    IZAWA Shunji, YAMAJI Kozo, YOKOYAMA Akihito, MATSUZAKI Kumiko, OHARA Naoko, YOSHIYAMA Masahiro

    Journal of Oral Tissue Engineering   13 ( 3 )   131 - 135   2016

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    Language:English   Publisher:Japanese Association of Regenerative Dentistry  

    Tissue regeneration is the important issue in the field of operative dentistry. In the previous study, we demonstrated that TNFα activates the potential for totipotency in human bone marrow mesenchymal stem cells (hMSCs) and leads to the regeneration of target cells or tissues along with the appropriate differentiating factor such as Emd-Gel. In this study, we apply the transcription factor set of TNF-α and Amelogenin to the human adipose-derived stem cells (hASCs) and our results indicate that this transcription factor set differentiate hASCs into odontblasts simillary and suggest that TNFα activates potential totipotency of hASCs and the activation can lead to the regeneration of target cells or tissues

    DOI: 10.11223/jarde.13.131

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  • Effects of Aging on Bone Formation Induced by BMP-2 using Low-intensity Pulsed Ultrasound

    YAMAJI Kozo, YOKOYAMA Akihito, MATSUZAKI Kumiko, OHARA Naoko, NISHIYAMA Eriko, SUGAYA Tsutomu, YOSHIYAMA Masahiro

    Journal of Oral Tissue Engineering   14 ( 1 )   65 - 69   2016

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    Language:English   Publisher:Japanese Association of Regenerative Dentistry  

    <p>The purpose of this study was to evaluate the effects of aging on bone formation induced by Bone Morphogenetic Protein-2 (BMP-2) using low-intensity pulsed ultrasound (LIPUS)in 12-week-old (12w group) and 70-week-old (70w group) Wistar rats.</p><p>The experimental sites (palatal subperiosteal sites) were divided into three sites as follows: BMP site, BMP-2 was implanted; LI-BMP site, LIPUS treatment was performed every three days after implantation of BMP-2; and Control site, neither implantation of BMP-2 nor LIPUS treatment was done.</p><p>At 3 weeks after surgery, thickness of new bone (TNB) was significantly higher in both the BMP and LI-BMP sites than in the Control site (P<0.05). TNB of every site in the 12w group was significantly higher than that of the same experimental site in the 70w group (P<0.05). Newly formed bone was almost integrated with original bone at the LI-BMP site.</p><p>These results suggest that LIPUS enhances the maturation of bone formation induced by BMP-2 even in senescent rats, although the volume of bone decreased with aging.</p>

    DOI: 10.11223/jarde.14.65

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  • ヒドロキシアパタイトを含む実験的接着剤を用いた長期間の樹脂のミネラル化(Mineralization of Resin Using Experimental Adhesives Containing Hydroxyapatite in Long Term)

    Hoshika Tomohiro, Nishitani Yoshihiro, Takahashi Kei, Ogata Haruka, Ohara Naoko, Kajihara Takehiro, Yoshiyama Masahiro

    Journal of Oral Tissue Engineering   13 ( 2 )   85 - 95   2015.12

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    Language:English   Publisher:日本再生歯科医学会  

    酸腐食象牙質への樹脂の浸透が不完全な場合は、ハイブリッド層に水分の多い、樹脂の少ない領域(隙間)が残ってしまい、樹脂や微細繊維の分解を引き起こすと推測される。実験的樹脂にヒドロキシアパタイト(Hap)を加えると、微小隙間をリン酸カルシウムで満すことができると考え、その仮説について検証した。0、5、10wt%のHApを加えた樹脂を作成した。この樹脂について、水分吸着と可溶性評価、カルシウム沈着誘導の評価、走査型電子顕微鏡観察とエネルギー分散X線分析、引張り試験を行った。その結果、HApは象牙質への樹脂の浸透には効果がなかったが、歯科用接着剤にHApを添加するとハイブリッド層の下に結晶を形成させ、強いアンカーになることが示唆された。

    DOI: 10.11223/jarde.13.85

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  • Influence of Incorporating Sodium Fluoride on Flexural Strength, Water Absorption and Solubility of Adhesive Resin Cements Reviewed

    Naoko OHARA, Kazuhiko SHIBUYA, Kumiko TANAKA, Tomohiro HOSHIKA, Azusa ENDO, Maiko NISHIMURA, Akiko TAKEUCHI, Kozo YAMAJI, Yoshihiro NISHITANI, Masahiro YOSHIYAMA

    日本歯科保存学雑誌   58 ( 1 )   10 - 16   2015.2

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    Authorship:Lead author   Language:Japanese   Publisher:(NPO)日本歯科保存学会  

    Purpose: By incorporating sodium fluoride in adhesive resin cement, the release of fluoride from the cement is expected to strengthen the teeth and prevent secondary caries. However, there is a concern that such fluoride incorporation and release could impair the physical properties of the cement. In this study, we investigated the influence of incorporating sodium fluoride on the flexural strength, water absorption and solubility of adhesive resin cement.<br> Materials and Methods: The experimental resin cements were prepared at concentrations of 0-20 wt% sodium fluoride. The amounts of fluoride released from the cements were measured from day 1 to day 84. Next, a three-point bending test of the experimental resin cements was performed after initial curing (24 hours storage in water at 37°C) and after thermal cycle loading (5°C-55°C, 10,000 times). Furthermore, water absorption and solubility following 30 days immersion in water were measured.<br> Results: Fluoride release increased with an increase in the content rate of sodium fluoride. After initial curing, the three-point bending strengths of 5 wt% and 10 wt% sodium fluoride compounded cement were not statistically decreased compared to those of sodium fluoride 0 wt%, but were significantly reduced by incorporation of more than 15 wt%. There were no significant differences in the strength between 15 wt% sodium fluoride compounded cement and 20 wt% sodium fluoride compounded cement. Similar results were also observed in the comparison of flexural strength after thermal cycle loading. Regarding the 30-day water absorption amount, there was no significant difference between 5 wt% sodium fluoride compounded cement and that of 0 wt% sodium fluoride cement. However, the 30-day water absorption of 10 wt% sodium fluoride compounded cement was significantly increased compared to that of 0 wt% sodium fluoride compounded cement. The solubility was not significantly different between the content rate of 0-10 wt% sodium fluoride.<br> Conclusion: Increasing the sodium fluoride content improved the amount of fluoride release. On the other hand, the flexural strength of the cement was decreased, and the amounts of water absorption and solubility were increased. Within the limit of this study, it is suggested that 10 wt% sodium fluoride compounded cement could provide fluoride release without decreasing mechanical strength.

    DOI: 10.11471/shikahozon.58.10

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    Other Link: http://search.jamas.or.jp/link/ui/2015218122

  • 垂直歯根破折歯を口腔外接着再植法にて保存した症例 Reviewed

    33 ( 1 )   7 - 11   2014.6

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    Authorship:Lead author   Language:Japanese   Publisher:岡山歯学会  

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  • D-Glucosamine Promotes Transfection Efficiency during Electroporation Reviewed

    Kazunari Igawa, Naoko Ohara, Atsushi Kawakubo, Kouji Sugimoto, Kajiro Yanagiguchi, Takeshi Ikeda, Shizuka Yamada, Yoshihiko Hayashi

    BIOMED RESEARCH INTERNATIONAL   2014   485867   2014

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:HINDAWI LTD  

    D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation with or without D-glucosamine are used in experiments of transfection vectors. This is the first study to indirectly observe the stability and protection of the osteoblast membrane against both electric stress and gene uptake (the proton sponge hypothesis: osmotic rupture during endosomes prior to fusion with lysosomes) in electroporation with D-glucosamine application. The transfection efficiency was evaluated as the fluorescence intensity of the transfected green fluorescent protein (GFP) in the cultured cells (osteoblasts; NOS-1 cells). The transfection efficiency increased over 30% in the electroporation samples treated with D-glucosamine-supplemented buffer after one day. The membrane absorption of D-glucosamine is the primary mechanism of membrane stress induced by electric stress. This new function of D-glucosamine is useful and meaningful for developing more effective transformation procedures.

    DOI: 10.1155/2014/485867

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  • 嚥下障害を有する岡山大学病院頭頸部がんセンターの患者に対して厳重なラバーダム装着がコンポジットレジン修復に有効であった症例 Reviewed

    田中久美子, 西谷佳浩, 大原直子, 吉山昌宏

    岡山歯学会雑誌   32 ( 2 )   65 - 70   2013

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  • Effects of Low-intensity Pulsed Ultrasound on Bone Formation Induced by Recombinant Human BMP-2 in Adult Rats Reviewed

    YAMAJI Kozo, SUGAYA Tsutomu, KAWANAMI Masamitsu, YOSHIYAMA Masahiro, SHIODE Shintaro, ODAJIMA Tomoomi, NISHITANI Yoshihiro, IZAWA Shunji, TANAKA Kumiko, OHARA Naoko, NISHIMURA Maiko, YOKOYAMA Akihito

    The Japanese Journal of Conservative Dentistry   56 ( 4 )   318 - 324   2013

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

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  • Observation of the Resin-dentin Interfacial Ultrastructure Using Confocal Laser Scanning Microscope with Fluorescent Dye Reviewed

    TANAKA Kumiko, NISHITANI Yoshihiro, OHARA Naoko, SHIBUYA Kazuhiko, YOSHIYAMA Masahiro

    The Japanese Journal of Conservative Dentistry   56 ( 4 )   310 - 317   2013

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    Purpose: The purpose of this study was to evaluate penetration of self-etching primer to human sound dentin using confocal laser scanning microscope (CLSM) with fluorescent dye. Methods: The fluorescent dye Rhodamine B was mixed into self-etching primer of Clearfil Mega Bond FA (Kuraray Noritake Dental) at the concentration of 0, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1%, or 0.2%. All dentin surfaces were treated with Clearfil Mega Bond FA. Resin composites (Clearfil AP-X) were built up incrementally according to the manufacturer's instruction. 64 specimens were subjected to the observation of the interface of resin and dentin with CLSM and SEM. The 32 specimens were then serially sectioned into multiple sliced parts and then micro-tensile bond strength (MTBS) was measured in a universal testing machine. Results: Penetration of monomer into dentin via dentinal tubules was observed as moderate in the group of 0.07, 0.08, 0.09% and 0.1%. On the other hand, in the group of 0.2%, the color of Rhodamine B was too dark to identify the details of the dentinal tubules. There was no significant difference in the MTBS between the group of 0.05, 0.06 or 0.07% and control (p>0.05). The MTBS produced by the group 0.08, 0.09, 0.1% or 0.2% were significantly different from control (p<0.05). Conclusion: This study suggested that the application of CLSM was effective to evaluate penetration of self-etching primer to dentin. A high concentration of Rhodamine B influenced the bond strength. It was suggested that the appropriate concentration of Rhodamine B was 0.07% in the Mega Bond FA Primer.

    DOI: 10.11471/shikahozon.56.310

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  • Surface roughness and gloss of indirect composites etched with acidulated phosphate fluoride solution Reviewed

    Naoko Ohara, Hiroyasu Koizumi, Yoshifumi Matsumoto, Daisuke Nakayama, Tomohisa Ogino, Hideo Matsumura

    ACTA ODONTOLOGICA SCANDINAVICA   67 ( 5 )   313 - 320   2009.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS AS  

    Objective. To evaluate the surface characteristics of indirect composites etched with acidulated phosphate fluoride (APF) solution. Material and methods. APF solution was applied to specimens of six composite materials. The surface morphological change was measured with a confocal laser scanning microscope. Gloss measurement and scanning electron microscope (SEM) observation were also performed. Results. The APF-treated surface showed various patterns due to the difference in the filler incorporated in each composite. The surface properties could be evaluated precisely from the surface roughness parameters. The arithmetical mean deviation of roughness profile (Ra) parameter of the specimens treated with APF solution was higher than those of the specimens untreated with APF, except New Meta Color Infis (IF). The difference in etching influence of each composite material was shown conclusively in the maximum height of the profile (Rz) parameter. Gradia (GR) and Gradia Forte (GF) were etched three times more deeply than that of IF categorized microfilled composite. The mean width of the profile element (RSm) decreased significantly after APF treatment, except in IF. Gloss was reduced apparently in all materials, indicating that gloss reduction was sensitive to slight surface changes. Conclusions. Specific filler particles of prosthodontic composite materials were etched by APF application. The surface roughness parameters, such as Rz and RSm, properly described various surface topographic features. Gloss was strongly correlated to surface roughness, as defined by these parameters, and especially to the initial change of surface roughness.

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  • Bond strength to bovine dentin of a composite core build-up material combined with four different bonding agents. Reviewed

    Ishii T, Ohara N, Oshima A, Koizumi H, Nakazawa M, Masuno T, Matsumura H

    Journal of Oral Science   50 ( 3 )   329 - 333   2008.9

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    Clearfil DC Bond (DC) is a new single-step, dual-cure bonding agent. In this study, the shear bond strengths of a core build-up composite to dentin used with four bonding systems [DC, Unifil Core Self-Etching Bond (UC), Clearfil SE Bond (SE) and Cleafil tri-S Bond (TS)] were measured. The bonding ability after 7 days of storage and in vitro durability following 20,000 thermocycles were also evaluated. The bond strength of DC did not differ significantly from those of other bonding systems after 24 hours of storage. Another dual-cure bonding system, UC, showed a significant reduction of bond strength after 7 days of storage. On the other hand, the bond strength of TS, a light-cured bonding system with a similar composition to DC, was reduced significantly following 20,000 thermocycles. SE, a two-step light-cure bonding system in the same series as DC, provided superior bond strength under all conditions. Although DC showed a slightly lower bond strength than SE, there was no significant difference between DC and SE under all conditions. Consequently, DC may be a useful and effective bonding system for multiple composite resin restorations. (J. Oral Sci. 50, 329-333, 2008)

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  • Chitosan monomer accelerates alkaline phosphatase activity on human osteoblastic cells under hypofunctional conditions Reviewed

    Shizuka Yamada, Tomoko Ganno, Naoko Ohara, Yoshihiko Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   83A ( 2 )   290 - 295   2007.11

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    Chitosan is a natural polyaminosaccharide that is extensively applied as an antitumor and antirheumatic drug. However, there are few reports about its effects on hypofunctional osteoblasts in vitro. We investigated the biological characteristics of a human osteoblastic cell line (NOS-1 cells) that was cultured with a chitosan monomer-containing medium under simulated microgravity conditions. After 7 days of cell incubation under the conventional conditions, the flasks were transferred to a microgravity simulator for 3 days. In the 0.005% chitosan monomer supplemented group, the marker enzyme of biological mineralization, the alkaline phosphatase (ALP) activity, was significantly higher compared with the control group (p &lt; 0.05). A cDNA microarray was performed to investigate the effects on the mRNA level by chitosan monomer, and the fluorescent signal was analyzed. The interferon gamma (IFN-gamma) receptor gene was detected with a signal ration of 2.2. The slight increase of IFN-gamma receptor expression was confirmed after 3 days of incubation according to RT-PCR analysis. Western blot analysis also showed the increased expression of IFN-gamma receptor. These results suggest that a supra-low concentration of chitosan monomer may increase the ALP activity of osteoblastic cells through the IFN-gamma receptor at the early phase of cell culture and recover the activity for biological mineralization under the hypofunctional condition. (c) 2007 Wiley Periodicals, Inc.

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  • Chitosan-containing gum chewing accelerates antibacterial effect with an increase in salivary secretion Reviewed

    Y. Hayashi, N. Ohara, T. Ganno, H. Ishizaki, K. Yanagiguchi

    JOURNAL OF DENTISTRY   35 ( 11 )   871 - 874   2007.11

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    Objectives: This study was designed to confirm the mechanical efficacy of chewing chitosan containing gum to suppress the growth of oral bacteria compared to a mouth rinse, and to demonstrate the increased salivary secretion due to chewing chitosan-containing gum.
    Method: Twelve healthy subjects were recruited from among the staff and students of Nagasaki University School of Dentistry. For the slab of gum study, the subjects chewed chitosan-containing gum for 5 min and then rested for 5 min. For the testing of the chitosan mouth rinse solution, the subjects gargled 10 mL of solution for 30 s followed by resting for 9 min 30 s. These protocols were continuously repeated five times for 50 min on the same day. For the salivary secretion experiment, the gum chewing was repeated three times per day for 2 days.
    Results: The amount of oral bacteria in the subjects who chewed chitosan-containing gum significantly decreased during all three sampling times except at 60 min for total bacteria in comparison to those in the rinse group. Chitosan-containing gum chewing also significantly increased the secretion of saliva.
    Conclusions: Chitosan-containing gum chewing has a greater antibacterial effect and it also increases salivary secretion. The present findings strongly indicate that the application of natural materials such as chitosan is useful for both oral health and the quality of life. (C) 2007 Elsevier Ltd. All rights reserved.

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  • Early gene expression analyzed by cDNA microarray and real-time PCR in osteoblasts cultured with chitosan monomer Reviewed

    Tomoko Ganno, Shizuka Yamada, Naoko Ohara, Tsunenori Matsunaga, Kajiro Yanagiguchi, Takeshi Ikeda, Hidetaka Ishizaki, Yoshihiko Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   82A ( 1 )   188 - 194   2007.7

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    Chitosan has a variety of biological activities. Although it has been reported that chitosan promotes osteogenesis in bone lesions, little is known about how it modulates the hard tissue forming cells at the gene level. This study focused on gene expressions in osteoblasts cultured with a super-low concentration of chitosan monomer. cDNA probes were synthesized from isolated RNA and labeled with fluorescent dye. They were hybridized with Human 3.8 II cDNA microarray, and the fluorescent signal was analyzed. cDNA microarray analysis revealed that 10 genes concerning to various signaling-related molecules were expressed at &gt;= 2.0-fold higher signal ratio levels in the experimental group when compared with the control group after 3 days. Real-time PCR analysis showed that chitosan monomer induced an increase in the expression of four signal transduction genes, mitogen-activated protein kinase (MAPK)K3, MAPKKK11, Rac1 and Shc1, together with the alkaline phosphatase gene. These results suggest that a super-low concentration of chitosan monomer could modulate the activity of osteoblastic cells through mRNA levels and that chitosan monomer directly affects signal transduction inside cells. (c) 2007 Wiley Periodicals, Inc.

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  • Chewing chitosan-containing gum effectively inhibits the growth of cariogenic bacteria Reviewed

    Yoshihiko Hayashi, Naoko Ohara, Tomoko Ganno, Keiji Yamaguchi, Takahide Ishizaki, Taisuke Nakamura, Makoto Sato

    ARCHIVES OF ORAL BIOLOGY   52 ( 3 )   290 - 294   2007.3

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    Objective: We have already reported that chitosan inhibited the growth of cariogenic bacteria in vitro. This study was designed to evaluate whether chewing gum, containing chitosan, can effectively suppressed the growth of oral bacteria (total bacteria, total Streptococci, mutans streptococci (MS)) in saliva.
    Methods: Fifty healthy subjects, ranging in age from 19 to 32 years, were recruited from among the staff and students of Nagasaki University School of Dentistry. For the slab of gum study, the subjects chewed gum for S min and then rested for 5 min. Each subject chewed a total of eight pieces of gum, which was either supplemented with or without chitosan, for a total of 80 min. Two different types of gums were examined with at least 1 week as a rest period in between treatments. This in vivo study was carried out by the double blind comparison test.
    Results: The amount of oral bacteria was found to significantly decrease in the chitosan group. Especially, the number of MS were maintained at about a 20% level in comparison to that before gum chewing, even at 1 h after gum chewing.
    Conclusion: These findings suggest that a supplementation of chitosan to gum is an effective method for controlling the number of cariogenic bacteria in situations where it is difficult to brush one's teeth, such as when an individual is away from home all day or participating in outdoor training. (c) 2006 Elsevier Ltd. All rights reserved.

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  • クラウン・ブリッジ科における臨床研修歯科医師のローテーション研修について Reviewed

    桟 淑行, 會田有希子, 小泉寛恭, 小峰 太, 大橋儀寿, 大島亜希子, 大原直子, 古地美佳, 萩原芳幸, 松村英雄

    日大歯学   81 ( 4 )   227-231   2007

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  • Chitosan monomer promotes tissue regeneration on dental pulp wounds Reviewed

    T Matsunaga, K Yanagiguchi, S Yamada, N Ohara, T Ikeda, Y Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   76A ( 4 )   711 - 720   2006.3

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    The present study was undertaken to evaluate the applicability of chitosan monomer (D-glucosamine hydrochloride) as a Pulp capping medicament. Both hi vitro and in vivo experiments were carried out to study the cell metabolism and Wound healing mechanisms following the application of chitomonosaccharide. After 3 days of osteoblast culture, alkaline phosphatase (ALP) activity Significantly increased in the chitosan group. Reverse transcription polymerase chain reaction analysis revealed that chitosan induced an increase in the expression of ALP mRNA after 3 days and bone morphogenetic protein-2 mRNA after 7 days of osteoblast incubation. Inflammatory cytokine, interleukin (IL)-8, synthesis in fibroblasts was strongly suppressed in the medium Supplemented with chitosan monomer. Histopathological effects were evaluated in rat experiments. After day, inflammatory cell infiltrations were observed to be weak when compared with the application of chitosan polymer. After 3 days, a remarkable proliferation of fibroblasts was seen near the applied chitosan monomer. The inflammatory cell infiltration had almost completely disappeared. After 5 days, the fibroblastic proliferation progressed, and some odontoblastic cells appeared at the periphery of the proliferated fibroblasts. These findings indicate that the present study is the first report that chitosan monomer acts as a biocompatibly stable medicament even at the initial Stage Of wound healing in comparison with the application of chitosan polymer. (c) 2005 Wiley Periodicals, Inc.

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  • Immunohistochemical and electron microscopic study of the biodegradation processes of chitin and chitosan implanted in rat alveolar bone Reviewed

    Ikeda T, Yanagiguchi K, Matsunaga T, Yamada S, Ohara N, Ganno T, Hayashi Y

    Oral Med Pathol   10 ( 4 )   131 - 138   2005

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  • Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide Reviewed

    N Ohara, Y Hayashi, S Yamada, SK Kim, T Matsunaga, K Yanagiguchi, T Ikeda

    BIOMATERIALS   25 ( 10 )   1749 - 1754   2004.5

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    Chitosan has a variety of biological activities. However, little is known about how chitosan modulates the hard tissue forming cells. When we cultured an osteoblastic cell line in alpha-MEM supplemented with 10% FBS and 0.005% chitooligosaccharide for 3 days, alkaline phosphatase (ALP) activity was significantly high compared with the control culture group (P &lt; 0.05). This study was focused on gene expression in osteoblasts cultured with water-soluble chitooligosaccharide. cDNA probes were synthesized from isolated RNA and labeled with fluorescent dye. They were hybridized with Human 1.0(R) cDNA microarray, and fluorescent signal was analyzed. cDNA microarray analysis revealed that 16 genes were expressed at greater than or equal to 1.5-fold higher signal ratio levels in the experimental group compared with the control group after 3 days. RT-PCR analysis showed that chitosan oligomer induced an increase in the expression of two genes, CD56 antigen and tissue-type plasminogen activator. Furthermore, the expression of mRNAs for BMP-2 was almost identical in the experimental and control groups after 3 days of culture, but slightly increased after 7 days of culture with chitosan oligomer. These results suggest that a super-low concentration of chitooligosaccharide could modulate the activity of osteoblastic cells through mRNA levels and that the genes concerning cell proliferation and differentiation call be controlled by water-soluble chitosan. (C) 2003 Elsevier Ltd. All rights reserved.

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  • Inhibitory effect of water-soluble chitosan on growth of Streptococcus mutans Reviewed

    M Fujiwara, Y Hayashi, N Ohara

    MICROBIOLOGICA   27 ( 1 )   83 - 86   2004.1

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    The present study was undertaken to evaluate the effects of pH and the degree of polymerization of chitosan on the inhibition of growth of Streptococcus mutans. Three types of chitosan, polymer, oligomer and monomer were used at 4% (W/V) and three different levels of pH: 6.0, 6.5 and 7.4. Bactericidal activity was calculated by the growth ratio. Chitosan oligomer significantly inhibited bacterial growth at a pH value of 6.5. All three types of chitosan strongly inhibited bacterial growth at pH 6.0. Furthermore, nearly complete inhibition was obtained with 2%(W/V) chitosan solution at constant pH 6.5. This study is the first to report that water-soluble chitosan directly suppresses the growth of the typical cariogenic bacterium S. mutans even at pH 6.5, without causing demineralization of the tooth surface.

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  • Mineralization of matrix vesicles isolated from a human osteosarcoma cell line in culture with water-soluble chitosan-containing medium Reviewed

    S Yamada, N Ohara, Y Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   66A ( 3 )   500 - 506   2003.9

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    Chitosan is a natural bioactive material. Although it has been reported that chitosan promotes osteogenesis in bone lesions, little is known about how chitosan modulates this process. The present study was designed to investigate the effect of water-soluble chitosan relative to initiation of biologic mineralization, especially in the matrix-vesicles-(MVs) mediated process in vitro. A human osteoblastic cell line (NOS-1) was used. After 3 days of incubation, the number of cells and alkaline phosphatase (ALP) activity increased significantly in the chitosan group. RTPCR analysis revealed that chitosan induced an increase in the expression of bone morphogenetic protein-2 mRNA after 7 days of incubation. MVs were isolated from NOS-1 cells using a collagenase digestion and ultracentrifugation method. ALP activity of MVs isolated from chitosan-supplemented cells was significantly higher than that of the control group. Furthermore, isolated MVs were incubated in medium supplemented with Na-beta-glycerophosphate without fetal bovine serum. Needle-like crystals were observed in association with MVs after 24h of incubation. These needle-like crystals were densely accumulated in the chitosan group. The present findings suggest that water-soluble chitosan would promote osteoblast proliferation and differentiation and may be useful for the acceleration of initial biologic mineralization.

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  • 根管治療時の臨床症状と根管内滲出液中の起炎性サイトカイン(IL-1β)濃度との関連性 Reviewed

    柳口嘉治郎, 山口慶治, 大原直子, 林 善彦

    日本歯科保存学会雑誌   45 ( 2 )   343 - 348   2002.4

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  • Expression of alkaline phosphatase induces rapid and artificial mineralization in specific transformed Escherichia coli Reviewed

    N Ohara, N Ohara, K Yanagiguchi, S Yamada, IL Viloria, Y Hayashi

    MICROBIOLOGICA   25 ( 1 )   107 - 110   2002.1

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    Matrix vesicles (MV) having high alkaline phosphatase (ALP) activity act as initiators of biological mineralization. Although bacteria have similar membranous structures to MV. ALP mediated mineralization has not been studied in bacterial cells. Escherichia coli was transformed with a bacterial ALP gene in this study. Recombinant E. coli overproducing ALP induced mineralization through hydrolysis of calcium-glycerophosphate (Ca-GP). Fourier transform infrared spectroscopy and electron microscopy combined with electron diffraction revealed newly formed hydroxyapatite mineral deposits. These findings suggest that hydrolysis of Ca-GP through ALP induced high Ca and Pi concentrations within bacterial cells followed by complete bacterial mineralization.

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  • The 16-kDa alpha-crystallin-like protein of Mycobacterium bovis BCG is produced under conditions of oxygen deficiency and is associated with ribosomes Reviewed

    Y Tabira, N Ohara, N Ohara, H Kitaura, S Matsumoto, M Naito, T Yamada

    RESEARCH IN MICROBIOLOGY   149 ( 4 )   255 - 264   1998.4

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    A 16-kDa protein, identical to the a-crystallin-like stress protein, was induced under O-2-deficient culture conditions and bound principally to the 30S ribosomal subunits of Mycobacterium bovis BCG substrain Tokyo (BCG). The 16-kDa protein was shown to be tightly associated with the ribosome.

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  • Characterization of the transcriptional initiation regions of genes for the major secreted protein antigens 85C and MPB51 of Mycobacterium bovis BCG Reviewed

    N Ohara, T Nishiyama, N Ohara-Wada, S Matsumoto, T Matsuo, T Yamada

    MICROBIAL PATHOGENESIS   23 ( 5 )   303 - 310   1997.11

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    The component of mycobacterial 85 complex (85A, 85B, and 85C) and MPB51 are very important from immunological, biochemical, and antimycobacterial points of view. In this study, the transcriptional properties of genes encoding three components of 85 complex and MPB51 from BCG were analysed. The authors' analyses revealed that genes for 85A and MPB51 were transcribed as a single unit despite the one operon-like structure and these four genes were probably under a different regulatory control. These findings may help to understand the immunological and physiological roles of these antigens. (C) 1997 Academic Press Limited.

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  • HrpA, a new ribosome-associated protein which appears in heat-stressed Mycobacterium bovis Bacillus Calmette-Guerin Reviewed

    N Ohara, N Ohara, M Naito, C Miyazaki, S Matsumoto, Y Tabira, T Yamada

    JOURNAL OF BACTERIOLOGY   179 ( 20 )   6495 - 6498   1997.10

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    A novel 18-kDa heat shock protein, HrpA, has been identified from Mycobacterium bovis BCG. HrpA was rapidly synthesized in membrane and ribosome fractions but not in the cytoplasmic fraction under heat shock stress. HrpA bound tightly to 70S ribosomes, mainly in 30S subunits. HrpA might be involved in the initiation step of translation at high temperature.

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  • Analysis of the genes encoding the antigen 85 complex and MPT51 from Mycobacterium avium Reviewed

    N Ohara, N Ohara-Wada, H Kitaura, T Nishiyama, S Matsumoto, T Yamada

    INFECTION AND IMMUNITY   65 ( 9 )   3680 - 3685   1997.9

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    The components of the fibronectin-binding antigen 85 complex (85A, 85B, and 85C) and the related protein MPB/MPT51 are major secreted proteins in Mycobacterium tuberculosis and Mycobacterium bovis BCT;, The fbpA, fbpC, and mpt51 genes encoding 85A, 85C, and MPT51, respectively, were isolated from Mycobacterium avium and sequenced in this study, The structures of these genes, and that of the fbpB gene encoding the 85B protein, were conserved in these three species. The secreted amounts of 85A, 85B, 85C, and MPB/MPT51 were compared for M. tuberculosis, BCG, and M, avium, These four proteins were found in large amounts in the culture filtrates from M. tuberculosis and BCG, In contrast, in the culture filtrate from M. avium, 85B and MPT51 were abundant whereas 85A and 85C were hardly found, in spite of the presence of the encoding genes, The difference in the secretion amounts might be regulated at the transcription level, These facts might reflect host immunopathogenesis, the protective immunities against infections, and the drug susceptibilities of these organisms.

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  • Long-lasting immune response induced by recombinant Bacillus Calmette-Guerin (BCG) secretion system Reviewed

    N Wada, N Ohara, M Kameoka, Y Nishino, S Matsumoto, T Nishiyama, M Naito, H Yukitake, Y Okada, K Ikuta, T Yamada

    SCANDINAVIAN JOURNAL OF IMMUNOLOGY   43 ( 2 )   202 - 209   1996.2

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    The recombinant bacillus Calmette-Guerin (rBCG) secretion system utilizing an extracellular alpha antigen of Mycobacterium kansasii (alpha-K) was characterized biochemically and immunologically. The human immunodeficiency virus type1 (HIV-1)p17(gag) B cell epitope fused to alpha-K was secreted in extremely large amounts. At least three mice out of seven inoculated with rBCG generated high titres of antibody to the epitope. The long-lasting antibody production persisted more than 14 months.

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  • Stable expression and secretion of the B-cell epitope of rodent malaria from Mycobacterium bovis BCG and induction of long-lasting humoral response in mouse

    S Matsumoto, T Yanagi, N Ohara, N Wada, H Kanbara, T Yamada

    VACCINE   14 ( 1 )   54 - 60   1996.1

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    The live bacterial vaccine Mycobacterium bovis BCG (BCG) is a vehicle worth noticing for various protective antigens. The gene encoding the B-cell epitope of the oligopeptide repeating in the circumsporozoite protein (C.S. protein) of the rodent malaria parasite, Plasmodium yoelii, was inserted into the plasmid vector under the control of an expression cassette carrying the promoter and signal sequence of the a antigen derived from Mycobacterium kansasii (k-a). The B-cell epitope was successfully expressed and secreted from BCG as a fusion protein with k-a. This recombinant BCG was administered subcutaneously into BALB/c mice and the antibody production was measured by the enzyme-linked immunosorbent assay (ELISA). Long lasting humoral response was found in one of seven mice.

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  • Characterization of the gene encoding the MPB51, one of the major secreted protein antigens of Mycobacterium bovis BCG, and identification of the secreted protein closely related to the fibronectin binding 85 complex. International journal

    N Ohara, H Kitaura, H Hotokezaka, T Nishiyama, N Wada, S Matsumoto, T Matsuo, M Naito, T Yamada

    Scandinavian journal of immunology   41 ( 5 )   433 - 42   1995.5

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    The secreted protein MPB51 is one of the major proteins in the culture filtrate of Mycobacterium bovis BCG (BCG) and is a protein immunologically cross-reacting with the fibronectin binding 85 complex secreted by this bacterium. The gene encoding MPB51 (mpb51) was cloned, sequenced, and expressed in Escherichia coli. The mpb51 gene was mapped downstream of the gene for 85A component with 179 bp spaces. The mpb51 gene encoded 299 amino acids, including 33 amino acids for the signal peptide, followed by 266 amino acids for the mature protein with a molecular mass of 27807.37 Da. This is the first complete sequence of MPB51. MPB51 showed 37-43% homology to the components of 85 complex. Two-dimensional electrophoresis of culture fluids of BCG and Western blotting indicated the existence of the other novel protein(s) which strongly cross-reacted with the alpha antigen (85B) and MPB51.

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  • Biochemical and Immunological Characterization of Ribosomal Fraction and Culture Filtrate from Mycobacterium Reviewed

    Yamada T, Ohara N, Wada N, Matsumoto S, Yukitake E, Matsuo T, Kitaura H, Takano M, Nishiyama T, Nomaguchi H, Matsuo M

    Actinomycetol   9 ( 2 )   228 - 235   1995

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    DOI: 10.3209/saj.9_228

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  • Structure and immunological properties of major secreted antigen a and related antigens of mycobacteria. Reviewed

    Ohara N, Kitaura H, Naito M, Nishiyama T, Wada N, Matsumoto S, Matsuo T, Hotokezaka H, Hayashida H, Yamada T

    Procceedings to 4th Western Pacifuc Congress on Chemotherapy and Infectious Diseases   10 ( 3 )   628 - 629   1994

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  • CLONING AND SEQUENCING OF THE GENE ENCODING THE RIBOSOMAL L7/L12-LIKE PROTEIN OF MYCOBACTERIUM-BOVIS BCG

    N OHARA, M KIMURA, N WADA, T YAMADA

    NUCLEIC ACIDS RESEARCH   21 ( 15 )   3579 - 3579   1993.7

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  • 【コンポジットレジン、1ステップか、2ステップか?操作の簡便さか?接着性と耐久性重視か?】(PART 2)接着性と耐久性重視の従来の2ステップボンディングシステムと新規2ステップボンディングシステム

    吉山 昌宏, 大原 直子

    The Quintessence   40 ( 10 )   2462 - 2467   2021.10

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  • Bond durability of a novel two-step bonding agent to dentin

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松崎久美子, 山路公造, 吉山昌宏

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)   154th   2021

  • 【歯髄保護の現状と新提案 保存修復の立場から再考する】根面う蝕修復の現状 コンポジットレジンVSグラスアイオノマーセメント

    吉山 昌宏, 松崎 久美子, 大原 直子, 横山 章人

    The Quintessence   39 ( 9 )   2122 - 2124   2020.9

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  • ジルコニア修復におけるMDP含有およびMDP非含有セルフアドヒーシブレジンセメントの微小剪断接着強さの評価

    垣内伸子, 大原直子, 吉山昌宏

    The Quintessence   39   236 - 237   2020

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  • 4%および10%過酸化水素を用いたホームホワイトニングの漂白効果と知覚過敏に対する無作為化三重盲検臨床試験

    小野瀬里奈, 大原直子, 吉山昌宏

    The Quintessence   37   200 - 201   2018

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  • BMP配合ゼラチンゲルをラット口蓋部に移植した場合の硬組織反応

    山路公造, 横山章人, 島田康史, 大原直子, 松崎久美子, 西山依理子, 田畑泰彦, 吉山昌宏

    日本再生歯科医学会学術大会および総会プログラム・抄録集   16th   2018

  • レジンコーティングを用いた臼歯部強化型グラスアイオノマーセメント修復の臨床評価に関する6年間の前向き研究

    澁谷和彦, 松崎久美子, 大原直子, 吉山昌宏

    The Quintessence   36   186 - 189   2017

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  • 各種CAD/CAMセラミックスの表面性状の相違による対合エナメル質の損耗

    岡村純子, 吉山昌宏, 大原直子, 星加知宏

    The Quintessence   34   181 - 189   2015

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  • セルフアドヒーシブレジンセメントで合着された間接法MODコンポジットレジンインレーの接着

    大原直子, 吉山昌宏

    the Quintessence   32 ( 7 )   210 - 211   2013

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  • 根管治療を行った大臼歯におけるコンポジットレジンとポーセレンCAD/CAMオーバーレイ修復の疲労抵抗性試験.

    大原直子, 吉山昌宏

    The Quintessence   30 ( 3 )   130 - 137   2011

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  • Biodegradation of Natural Organic Bioactive Material, "Chitosan" implanted into Rat Alveolar Bone

    IKEDA Takeshi, YANAGIGUCHI Kajirou, MASTUNAGA Tunenori, YAMADA Shizuka, OHARA Naoko, GANNOU Tomoko, HAYASHI Yoshihiko

    47   215 - 215   2004.10

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  • Analysis of Osteoblastic Cell's Response in Culture with Chitosan Monomer using cDNA Microarray

    YAMADA Shizuka, GANNO Tomoko, OHARA Naoko, HAYASHI Yoshihiko

    47   121 - 121   2004.10

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  • The acceleration of biological mineralization on a human osteosarcoma cell line in culture with water-soluble chitosan containing medium(Cellular & Tissue Engineering)

    Yamada Shizuka, Ohara Naoko, Hayashi Yoshihiko

    Proceedings of the ... Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics   2004 ( 1 )   93 - 94   2004.3

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  • Structural analyses on inhibitory effects of water-soluble chitosan on growth of Streptococcus mutans

    OHARA Naoko, MATSUBNAGA Tsunenori, YANAGIGUCHI Kajiro, HAYASHI Yoshihiko

    46   159 - 159   2003.10

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  • 細菌の人為的石灰化

    大原 直子, 大原 直也, 林 善彦

    歯科基礎医学会雑誌   42 ( 5 )   511 - 511   2000.8

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  • Characteristics of Mineralization in Osteoblast Cell Line Established from Human Osteosarcoma

    YAMADA Shizuka, OHARA Naoko, KAIDA Kei, HAYASHI Yoshihiko

    43   43 - 43   2000.3

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  • Application of Mineralizing Cariogenic Bacteria as Prevention and Initial Treatment of Dental Caries Part 1. Artificial Mineralization by Recombinant Escherichia coli Overproducing Alkaline Phosphatase -a Model System-

    OHARA Naoko, VILORIA Iluminada L., YANAGIGUCHI Kajiro, HAYASHI Yoshihiko

    41   95 - 95   1998.10

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  • 抗酸菌のストレス蛋白質-細胞外に分泌するHSPとリボゾ-ムに関わるHSP-

    大原 直也, 田平 泰広, 大原 直子

    長崎大学熱帯医学研究所共同研究報告集   9   109 - 109   1998.7

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  • Hydroxyapatite Applied as Direct Pulp Capping Medicament Substitutes for Osteodentine

    YANAGIGUCHI Kajiro, VILORIA Iluminada L., IKEDA Takeshi, OHARA Naoko, HAYASHI Yoshihiko

    41   30 - 30   1998.5

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  • BCG菌リボゾームに存在する熱応答性蛋白質の性質

    大原 直也, 大原 直子, 内藤 真理子, 山田 毅

    日本分子生物学会年会プログラム・講演要旨集   19   176 - 176   1996.8

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Presentations

  • The acceleration of biological mineralization on a human osteosarcoma cell line in culture with water-soluble chitosan containing medium

    Yamada S, Ohara N, Hayashi Y

    First Asian Pacific Conference on Biomechanics Abstracts  2004.3 

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  • The Application of Chitosan for Promoting Biological Mineralization

    Hayashi Y, Yamada S, Ohara N, Kim S-K, Ikeda T, Yanagiguchi K, Matsunaga T

    The 5th Joint-Scientific meeting between the Korean Academy of Conservative Dentistry and Japanese Society of Conservative Dentistry  2003.11 

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  • Gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide

    Ohara N, Hayashi Y, Yamada S, Matsunaga T, Kim S-K

    9th International Chitin-Chitosan Conference Montreal Abstracts  2003.8 

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  • The Behavior of Cultured Osteoblasts on Chitosan Sponge Supplemented with Organic Phosphate

    MATSUNAGA Tunenori, YANAGIGUCHI Kajiro, IKEDA Takeshi, YAMADA Shizuka, OHARA Naoko, HAYASHI Yoshihiko

    2003.5.6 

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    Event date: 2003.5.6

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  • Early Gene Expression Analyzed by cDNA Microarray in Osteoblasts Cultured with Water-Soluble Chitooligosaccharide

    Hayashi Y, Yamada S, S.-K. Kim, Ohara N, Yanagiguchi K, Matsunaga T, Ikeda T, Toda K

    International Congress on Biological and Medical Engineering (Singapore) Programme & Abstract Handbook  2002 

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    Event date: 2002

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  • The mineralization of matrix vesicles isolated from human sarcoma cell line in culture with water-soluble chitosan

    Yamada S, Ohara N, Hayashi Y

    3rd International Symposium on Chitin Enzymology and 4th Conference of the European Chitin Society Abstract Book  2001 

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  • 新規2ステップシステムにおけるボンド塗布後のエアーブロー圧が象牙質接着性に及ぼす影響

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松崎久美子, 山路公造, 吉山昌宏

    第155回日本歯科保存学会秋季学術大会  2021.10 

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  • 新規2ステップボンディング材の象牙質への接着耐久性

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松崎久美子, 山路公造, 吉山昌宏

    第154回日本歯科保存学会春季学術大会  2021.6 

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  • エストロゲン欠乏ラットにBMP配合ゼラチンゲルを移植した場合の硬組織反応

    横山 章人, 山路 公造, 吉山 知宏, 大原 直子, 松﨑 久美子, 島田 康史, 吉山 昌宏

    第18回日本再生歯科医学会学術大会  2020.11 

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  • 新規2ステップボンディング材の象牙質微小引張強さ

    大原直子, 小野瀬里奈, 澁谷和彦, 吉山昌宏

    第39回日本接着歯学会学術大会  2020.10 

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  • 塩化ストロンチウム配合象牙質知覚過敏抑制材の脱灰抑制効果

    松﨑久美子, 佐藤奈月, 神農泰生, 山路公造, 大原直子, 島田康史, Sadr Alireza, 角 保徳, 田上順次, 吉山昌宏

    第152回日本歯科保存学会春季学術大会  2020.6 

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  • Bonding performance evaluation of novel 2-step bonding agent on dentin.

    Ohara N, Yoshiyama M

    2020 IADR/AADR/CADR General Session & Exhibition  2020.3 

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  • 頭頚部癌化学放射線療法後に発症した放射線性う蝕の1例

    松﨑久美子, 大原直子, 澁谷和彦, 横山章人, 山路公造, 島田康史, 木股敬裕, 吉山昌宏

    第151回日本歯科保存学会秋季学術大会  2019.11 

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  • PCA配合歯磨剤のレジン象牙質接着性への影響の検討

    吉山昌宏, 大原直子, 松崎久美子

    第151回日本歯科保存学会秋季学術大会  2019.11 

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  • BMP配合アルギン酸ゲルを直接覆髄に用いた場合の硬組織形成

    横山章人, 山路公造, 塩出信太郎, 大原直子, 松﨑久美子, 島田康史, 吉山昌宏

    第151回日本歯科保存学会秋季学術大会  2019.11 

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  • 象牙質およびコア用レジンに対するシランカップリング剤含有レジンセメントの接着強さ

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松崎久美子, 山路公造, 吉山昌宏

    第150回日本歯科保存学会春季学術大会,金沢  2019.6 

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  • Effects of BMP-2 and LIPUS on bone formation at palatal sites in aged rats

    Yamaji K, Yokoyama A, Shimada Y, Ohara N, Yoshiyama M

    2019 IADR/AADR/CADR General Session  2019.6 

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  • Influence of silver diamine fluoride on bond strengths to caries-affected dentin

    Ohara N, Matsuzaki K, Shibuya K, Ono S, Yokoyama A, Yamaji K, Yoshiyama M

    2019 IADR/AADR/CADR General Session  2019.6 

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  • 着色象牙質を残置させた窩洞に対する新規コンポジットレジン(ECM-001)修復の色調評価

    小野瀬里奈, 大原直子, 澁谷和彦, 松崎久美子, 神農泰生, 島田康史, 吉山昌宏

    第150回日本歯科保存学会春季大会  2019.6 

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  • 充填用材料の硬化初期における吸水および乾燥が物性へ及ぼす影響

    澁谷和彦, 大原直子, 入江正郎, 島田康史, 松本卓也, 吉山昌宏

    第150回日本歯科保存学会春季学術大会  2019.6 

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  • BMP配合ゼラチンゲルをラット口蓋部に移植した場合の硬組織反応

    山路公造, 横山章人, 島田康史, 大原直子, 松崎久美子, 西山依理子, 田畑泰彦, 吉山昌宏

    第16回日本再生歯科医学会  2018.12 

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  • 化学重合型接着材ボンドマーライトレスを用いたコンポジットレジン修復の窩洞適合性のSS-OCT評価

    横山章人, 山路公造, 島田康史, 松崎久美子, 大原直子, 神農泰生, 高橋圭, Sadr Alireza, 角保徳, 田上順次, 吉山昌宏

    第149回日本歯科保存学会  2018.11 

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  • 新規コンポジットレジン(ECM-001)色調適合性の評価

    小野瀬里奈, 大原直子, 吉山知宏, 吉山昌宏

    第149回日本歯科保存学会  2018.11 

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  • フッ化ジアンミン銀塗布がう蝕象牙質の接着に及ぼす影響

    大原直子, 松﨑久美子, 澁谷和彦, 小野瀬里奈, 横山章人, 山路公造, 吉山昌宏

    第149回日本歯科保存学会秋季学術大会  2018.11 

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  • The effects of a PCA-containing dentifrice on resin bonding to human dentin

    Yoshiyama M, Ohara N, Matsuzaki K

    The 20th KACD-JSCD Joint Scientific Meeting  2018.11 

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  • 透明根管模型を使用した根管充填の評価に関する検討

    松崎久美子, 大原直子, 澁谷和彦, 横山章人, 岡田俊輔, 村上純, 久富美紀, 浅海淳一, 吉山昌宏

    第39回岡山歯学会学術大会  2018.10 

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  • The influence of dry and wet conditions on filling materials

    Shibuya K, Ohara N, Matsuzaki K, Shimada Y, Yoshiyama M

    96th IADR General Session  2018.7 

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  • 充填用コンポジットレジンの色調に関する検討

    大原 直子, 小野瀬 里奈, 松崎 久美子, 澁谷 和彦, 横山 章人, 神農 泰生, 山路 公造, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2017.5 

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  • セルフアドヒーシブセメント中のMDP濃度が接着強さに及ぼす影響

    大原 直子, 田中 久美子, 澁谷 和彦, 横山 章人, 竹内 晶子, 山路 公造, 西谷 佳浩, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2015.11 

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  • Actinomyces属3菌種による歯根象牙質う蝕の誘発

    大原 直子, 大原 直也, 田中 久美子, 山路 公造, 西谷 佳浩, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2014.6 

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  • 細菌バイオミネラリゼーション誘導の試み

    大原 直子, 大原 直也, 田中 久美子, 澁谷 和彦, 横山 章人, 山路 公造, 西谷 佳浩, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2013.10 

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  • 口腔連鎖球菌による歯根象牙質う蝕の誘発

    大原 直子, 田中 久美子, 澁谷 和彦, 西谷 佳浩, 大原 直也, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2012.10 

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Research Projects

  • surface roughness of indirect composite

    2007

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Class subject in charge

  • Clinical training: Advanced Esthetic Dentistry (2021academic year) special  - その他

  • Lecture and Research Projects: Advanced Esthetic Dentistry (2021academic year) special  - その他

  • Clinical training: Advanced Adhesive Dentistry (2021academic year) special  - その他

  • Lecture and Research Projects: Advanced Adhesive Dentistry (2021academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry I (2021academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry I (2021academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry II (2021academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry II (2021academic year) special  - その他

  • Diagnosis and treatment of Dentin/Pulp Complex (2021academic year) Fourth semester  - 金4

  • Clinical metal-based materials (2021academic year) Second semester  - 木1,木2

  • Cariology and Clinical adhesive materials (2021academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

  • Cariology and Clinical adhesive materials (2021academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

  • Clinical training: Advanced Esthetic Dentistry (2020academic year) special  - その他

  • Lecture and Research Projects: Advanced Esthetic Dentistry (2020academic year) special  - その他

  • Clinical training: Advanced Adhesive Dentistry (2020academic year) special  - その他

  • Lecture and Research Projects: Advanced Adhesive Dentistry (2020academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry I (2020academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry I (2020academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry II (2020academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry II (2020academic year) special  - その他

  • Diagnosis and treatment of Dentin/Pulp Complex (2020academic year) Fourth semester  - 金4

  • Clinical metal-based materials (2020academic year) Second semester  - 木1,木2

  • Cariology and Clinical adhesive materials (2020academic year) 1st semester  - 木1,木2

  • Cariology and Clinical adhesive materials (2020academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

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