Updated on 2021/12/02

写真a

 
OHARA Naoko
 
Organization
Medicine, Dentistry and Pharmaceutical Sciences Associate Professor
Position
Associate Professor
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Degree

  • D.D.S. ( Nagasaki University )

Research Interests

  • dental caries

  • composite resin

  • adhesive dentistry

Research Areas

  • Life Science / Conservative dentistry

Education

  • Nagasaki University    

    - 1996

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  • Nagasaki University   Graduate School of Dentistry  

    - 1996

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    Country: Japan

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Research History

  • Okayama University   大学病院 むしば科   Lecturer

    2017.5

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  • Okayama University   大学院医歯薬学総合研究科歯科保存修復学分野   Assistant Professor

    2010.4 - 2017.4

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  • -:Assistant Professor, Nihon University School of Dentistry Department of Fixed Prosthodontics

    2007.4 - 2010.3

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  • -:日本大学歯学部歯科補綴学教室Ⅲ講座助教

    2007.4 - 2010.3

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  • :Instructor, Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences

    2006 - 2007

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  • :長崎大学大学院医歯薬学総合研究科医療科学専攻展開医療科学講座

    2006 - 2007

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  • :長崎大学大学院医歯薬学総合研究科医療科学専攻発生分化機能再建学講座齲蝕学分野助手

    2002 - 2006

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  • :Instructor, Division of Cariology, Graduate School of Biomedical Sciences, Nagasaki University School of Dentistry

    2002 - 2006

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  • :長崎大学歯学部歯科保存学第一講座助手

    1997 - 2002

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  • :Instructor, Department of Endodontics and Operative Dentistry, Nagasaki University School of Dentistry

    1997 - 2002

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  • :長崎大学歯学部付属病院医員

    1997

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  • :Clinical Fellow, Department of Endodontics and Operative Dentistry, Nagasaki University School of Dentistry

    1997

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  • 歯科学群齲蝕学分野助手

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Professional Memberships

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Papers

  • フッ化ナトリウムの配合が接着性レジンセメントの曲げ強さと吸水および溶解に及ぼす影響 Reviewed

    大原 直子, 澁谷 和彦, 田中 久美子, 星加 知宏, 遠藤 梓, 西村 麻衣子, 竹内 晶子, 山路 公造, 西谷 佳浩, 吉山 昌宏

    日本歯科保存学雑誌   58 ( 1 )   10 - 16   2015.2

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    Language:Japanese   Publisher:(NPO)日本歯科保存学会  

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  • 垂直歯根破折歯を口腔外接着再植法にて保存した症例 Reviewed

    大原 直子, 田中 久美子, 垣内 伸子, 星加 知宏, 高橋 圭, 神農 泰生, 伊澤 俊次, 山路 公造, 西谷 佳浩, 吉山 昌宏

    岡山歯学会雑誌   33 ( 1 )   7 - 11   2014.6

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    Language:Japanese   Publisher:岡山歯学会  

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  • D-Glucosamine Promotes Transfection Efficiency during Electroporation Reviewed

    Kazunari Igawa, Naoko Ohara, Atsushi Kawakubo, Kouji Sugimoto, Kajiro Yanagiguchi, Takeshi Ikeda, Shizuka Yamada, Yoshihiko Hayashi

    BIOMED RESEARCH INTERNATIONAL   2014   485867   2014

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:HINDAWI LTD  

    D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation with or without D-glucosamine are used in experiments of transfection vectors. This is the first study to indirectly observe the stability and protection of the osteoblast membrane against both electric stress and gene uptake (the proton sponge hypothesis: osmotic rupture during endosomes prior to fusion with lysosomes) in electroporation with D-glucosamine application. The transfection efficiency was evaluated as the fluorescence intensity of the transfected green fluorescent protein (GFP) in the cultured cells (osteoblasts; NOS-1 cells). The transfection efficiency increased over 30% in the electroporation samples treated with D-glucosamine-supplemented buffer after one day. The membrane absorption of D-glucosamine is the primary mechanism of membrane stress induced by electric stress. This new function of D-glucosamine is useful and meaningful for developing more effective transformation procedures.

    DOI: 10.1155/2014/485867

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  • Surface roughness and gloss of indirect composites etched with acidulated phosphate fluoride solution Reviewed

    Naoko Ohara, Hiroyasu Koizumi, Yoshifumi Matsumoto, Daisuke Nakayama, Tomohisa Ogino, Hideo Matsumura

    ACTA ODONTOLOGICA SCANDINAVICA   67 ( 5 )   313 - 320   2009.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS AS  

    Objective. To evaluate the surface characteristics of indirect composites etched with acidulated phosphate fluoride (APF) solution. Material and methods. APF solution was applied to specimens of six composite materials. The surface morphological change was measured with a confocal laser scanning microscope. Gloss measurement and scanning electron microscope (SEM) observation were also performed. Results. The APF-treated surface showed various patterns due to the difference in the filler incorporated in each composite. The surface properties could be evaluated precisely from the surface roughness parameters. The arithmetical mean deviation of roughness profile (Ra) parameter of the specimens treated with APF solution was higher than those of the specimens untreated with APF, except New Meta Color Infis (IF). The difference in etching influence of each composite material was shown conclusively in the maximum height of the profile (Rz) parameter. Gradia (GR) and Gradia Forte (GF) were etched three times more deeply than that of IF categorized microfilled composite. The mean width of the profile element (RSm) decreased significantly after APF treatment, except in IF. Gloss was reduced apparently in all materials, indicating that gloss reduction was sensitive to slight surface changes. Conclusions. Specific filler particles of prosthodontic composite materials were etched by APF application. The surface roughness parameters, such as Rz and RSm, properly described various surface topographic features. Gloss was strongly correlated to surface roughness, as defined by these parameters, and especially to the initial change of surface roughness.

    DOI: 10.1080/00016350903070911

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  • Bond strength to bovine dentin of a composite core build-up material combined with four different bonding agents. Reviewed

    Ohara Naoko

    Journal of Oral Science   50 ( 3 )   329 - 333   2008.9

  • Chitosan monomer accelerates alkaline phosphatase activity on human osteoblastic cells under hypofunctional conditions Reviewed

    Shizuka Yamada, Tomoko Ganno, Naoko Ohara, Yoshihiko Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   83A ( 2 )   290 - 295   2007.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-LISS  

    Chitosan is a natural polyaminosaccharide that is extensively applied as an antitumor and antirheumatic drug. However, there are few reports about its effects on hypofunctional osteoblasts in vitro. We investigated the biological characteristics of a human osteoblastic cell line (NOS-1 cells) that was cultured with a chitosan monomer-containing medium under simulated microgravity conditions. After 7 days of cell incubation under the conventional conditions, the flasks were transferred to a microgravity simulator for 3 days. In the 0.005% chitosan monomer supplemented group, the marker enzyme of biological mineralization, the alkaline phosphatase (ALP) activity, was significantly higher compared with the control group (p < 0.05). A cDNA microarray was performed to investigate the effects on the mRNA level by chitosan monomer, and the fluorescent signal was analyzed. The interferon gamma (IFN-gamma) receptor gene was detected with a signal ration of 2.2. The slight increase of IFN-gamma receptor expression was confirmed after 3 days of incubation according to RT-PCR analysis. Western blot analysis also showed the increased expression of IFN-gamma receptor. These results suggest that a supra-low concentration of chitosan monomer may increase the ALP activity of osteoblastic cells through the IFN-gamma receptor at the early phase of cell culture and recover the activity for biological mineralization under the hypofunctional condition. (c) 2007 Wiley Periodicals, Inc.

    DOI: 10.1002/jbm.a.31234

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  • Chitosan-containing gum chewing accelerates antibacterial effect with an increase in salivary secretion Reviewed

    Y. Hayashi, N. Ohara, T. Ganno, H. Ishizaki, K. Yanagiguchi

    JOURNAL OF DENTISTRY   35 ( 11 )   871 - 874   2007.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI LTD  

    Objectives: This study was designed to confirm the mechanical efficacy of chewing chitosan containing gum to suppress the growth of oral bacteria compared to a mouth rinse, and to demonstrate the increased salivary secretion due to chewing chitosan-containing gum.
    Method: Twelve healthy subjects were recruited from among the staff and students of Nagasaki University School of Dentistry. For the slab of gum study, the subjects chewed chitosan-containing gum for 5 min and then rested for 5 min. For the testing of the chitosan mouth rinse solution, the subjects gargled 10 mL of solution for 30 s followed by resting for 9 min 30 s. These protocols were continuously repeated five times for 50 min on the same day. For the salivary secretion experiment, the gum chewing was repeated three times per day for 2 days.
    Results: The amount of oral bacteria in the subjects who chewed chitosan-containing gum significantly decreased during all three sampling times except at 60 min for total bacteria in comparison to those in the rinse group. Chitosan-containing gum chewing also significantly increased the secretion of saliva.
    Conclusions: Chitosan-containing gum chewing has a greater antibacterial effect and it also increases salivary secretion. The present findings strongly indicate that the application of natural materials such as chitosan is useful for both oral health and the quality of life. (C) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jdent.2007.08.004

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  • Early gene expression analyzed by cDNA microarray and real-time PCR in osteoblasts cultured with chitosan monomer Reviewed

    Tomoko Ganno, Shizuka Yamada, Naoko Ohara, Tsunenori Matsunaga, Kajiro Yanagiguchi, Takeshi Ikeda, Hidetaka Ishizaki, Yoshihiko Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   82A ( 1 )   188 - 194   2007.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-LISS  

    Chitosan has a variety of biological activities. Although it has been reported that chitosan promotes osteogenesis in bone lesions, little is known about how it modulates the hard tissue forming cells at the gene level. This study focused on gene expressions in osteoblasts cultured with a super-low concentration of chitosan monomer. cDNA probes were synthesized from isolated RNA and labeled with fluorescent dye. They were hybridized with Human 3.8 II cDNA microarray, and the fluorescent signal was analyzed. cDNA microarray analysis revealed that 10 genes concerning to various signaling-related molecules were expressed at >= 2.0-fold higher signal ratio levels in the experimental group when compared with the control group after 3 days. Real-time PCR analysis showed that chitosan monomer induced an increase in the expression of four signal transduction genes, mitogen-activated protein kinase (MAPK)K3, MAPKKK11, Rac1 and Shc1, together with the alkaline phosphatase gene. These results suggest that a super-low concentration of chitosan monomer could modulate the activity of osteoblastic cells through mRNA levels and that chitosan monomer directly affects signal transduction inside cells. (c) 2007 Wiley Periodicals, Inc.

    DOI: 10.1002/jbm.a.31130

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  • Chewing chitosan-containing gum effectively inhibits the growth of cariogenic bacteria Reviewed

    Yoshihiko Hayashi, Naoko Ohara, Tomoko Ganno, Keiji Yamaguchi, Takahide Ishizaki, Taisuke Nakamura, Makoto Sato

    ARCHIVES OF ORAL BIOLOGY   52 ( 3 )   290 - 294   2007.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: We have already reported that chitosan inhibited the growth of cariogenic bacteria in vitro. This study was designed to evaluate whether chewing gum, containing chitosan, can effectively suppressed the growth of oral bacteria (total bacteria, total Streptococci, mutans streptococci (MS)) in saliva.
    Methods: Fifty healthy subjects, ranging in age from 19 to 32 years, were recruited from among the staff and students of Nagasaki University School of Dentistry. For the slab of gum study, the subjects chewed gum for S min and then rested for 5 min. Each subject chewed a total of eight pieces of gum, which was either supplemented with or without chitosan, for a total of 80 min. Two different types of gums were examined with at least 1 week as a rest period in between treatments. This in vivo study was carried out by the double blind comparison test.
    Results: The amount of oral bacteria was found to significantly decrease in the chitosan group. Especially, the number of MS were maintained at about a 20% level in comparison to that before gum chewing, even at 1 h after gum chewing.
    Conclusion: These findings suggest that a supplementation of chitosan to gum is an effective method for controlling the number of cariogenic bacteria in situations where it is difficult to brush one's teeth, such as when an individual is away from home all day or participating in outdoor training. (c) 2006 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2006.10.004

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  • Chitosan monomer promotes tissue regeneration on dental pulp wounds Reviewed

    T Matsunaga, K Yanagiguchi, S Yamada, N Ohara, T Ikeda, Y Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   76A ( 4 )   711 - 720   2006.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-LISS  

    The present study was undertaken to evaluate the applicability of chitosan monomer (D-glucosamine hydrochloride) as a Pulp capping medicament. Both hi vitro and in vivo experiments were carried out to study the cell metabolism and Wound healing mechanisms following the application of chitomonosaccharide. After 3 days of osteoblast culture, alkaline phosphatase (ALP) activity Significantly increased in the chitosan group. Reverse transcription polymerase chain reaction analysis revealed that chitosan induced an increase in the expression of ALP mRNA after 3 days and bone morphogenetic protein-2 mRNA after 7 days of osteoblast incubation. Inflammatory cytokine, interleukin (IL)-8, synthesis in fibroblasts was strongly suppressed in the medium Supplemented with chitosan monomer. Histopathological effects were evaluated in rat experiments. After day, inflammatory cell infiltrations were observed to be weak when compared with the application of chitosan polymer. After 3 days, a remarkable proliferation of fibroblasts was seen near the applied chitosan monomer. The inflammatory cell infiltration had almost completely disappeared. After 5 days, the fibroblastic proliferation progressed, and some odontoblastic cells appeared at the periphery of the proliferated fibroblasts. These findings indicate that the present study is the first report that chitosan monomer acts as a biocompatibly stable medicament even at the initial Stage Of wound healing in comparison with the application of chitosan polymer. (c) 2005 Wiley Periodicals, Inc.

    DOI: 10.1002/jbm.a.30588

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  • Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide Reviewed

    N Ohara, Y Hayashi, S Yamada, SK Kim, T Matsunaga, K Yanagiguchi, T Ikeda

    BIOMATERIALS   25 ( 10 )   1749 - 1754   2004.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI LTD  

    Chitosan has a variety of biological activities. However, little is known about how chitosan modulates the hard tissue forming cells. When we cultured an osteoblastic cell line in alpha-MEM supplemented with 10% FBS and 0.005% chitooligosaccharide for 3 days, alkaline phosphatase (ALP) activity was significantly high compared with the control culture group (P < 0.05). This study was focused on gene expression in osteoblasts cultured with water-soluble chitooligosaccharide. cDNA probes were synthesized from isolated RNA and labeled with fluorescent dye. They were hybridized with Human 1.0(R) cDNA microarray, and fluorescent signal was analyzed. cDNA microarray analysis revealed that 16 genes were expressed at greater than or equal to 1.5-fold higher signal ratio levels in the experimental group compared with the control group after 3 days. RT-PCR analysis showed that chitosan oligomer induced an increase in the expression of two genes, CD56 antigen and tissue-type plasminogen activator. Furthermore, the expression of mRNAs for BMP-2 was almost identical in the experimental and control groups after 3 days of culture, but slightly increased after 7 days of culture with chitosan oligomer. These results suggest that a super-low concentration of chitooligosaccharide could modulate the activity of osteoblastic cells through mRNA levels and that the genes concerning cell proliferation and differentiation call be controlled by water-soluble chitosan. (C) 2003 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.biomaterials.2003.08.022

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  • Inhibitory effect of water-soluble chitosan on growth of Streptococcus mutans Reviewed

    M Fujiwara, Y Hayashi, N Ohara

    MICROBIOLOGICA   27 ( 1 )   83 - 86   2004.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:LUIGI PONZIO E FIGLIO  

    The present study was undertaken to evaluate the effects of pH and the degree of polymerization of chitosan on the inhibition of growth of Streptococcus mutans. Three types of chitosan, polymer, oligomer and monomer were used at 4% (W/V) and three different levels of pH: 6.0, 6.5 and 7.4. Bactericidal activity was calculated by the growth ratio. Chitosan oligomer significantly inhibited bacterial growth at a pH value of 6.5. All three types of chitosan strongly inhibited bacterial growth at pH 6.0. Furthermore, nearly complete inhibition was obtained with 2%(W/V) chitosan solution at constant pH 6.5. This study is the first to report that water-soluble chitosan directly suppresses the growth of the typical cariogenic bacterium S. mutans even at pH 6.5, without causing demineralization of the tooth surface.

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  • Mineralization of matrix vesicles isolated from a human osteosarcoma cell line in culture with water-soluble chitosan-containing medium Reviewed

    S Yamada, N Ohara, Y Hayashi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   66A ( 3 )   500 - 506   2003.9

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    Chitosan is a natural bioactive material. Although it has been reported that chitosan promotes osteogenesis in bone lesions, little is known about how chitosan modulates this process. The present study was designed to investigate the effect of water-soluble chitosan relative to initiation of biologic mineralization, especially in the matrix-vesicles-(MVs) mediated process in vitro. A human osteoblastic cell line (NOS-1) was used. After 3 days of incubation, the number of cells and alkaline phosphatase (ALP) activity increased significantly in the chitosan group. RTPCR analysis revealed that chitosan induced an increase in the expression of bone morphogenetic protein-2 mRNA after 7 days of incubation. MVs were isolated from NOS-1 cells using a collagenase digestion and ultracentrifugation method. ALP activity of MVs isolated from chitosan-supplemented cells was significantly higher than that of the control group. Furthermore, isolated MVs were incubated in medium supplemented with Na-beta-glycerophosphate without fetal bovine serum. Needle-like crystals were observed in association with MVs after 24h of incubation. These needle-like crystals were densely accumulated in the chitosan group. The present findings suggest that water-soluble chitosan would promote osteoblast proliferation and differentiation and may be useful for the acceleration of initial biologic mineralization.

    DOI: 10.1002/jbm.a.10009

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  • Expression of alkaline phosphatase induces rapid and artificial mineralization in specific transformed Escherichia coli Reviewed

    N Ohara, N Ohara, K Yanagiguchi, S Yamada, IL Viloria, Y Hayashi

    MICROBIOLOGICA   25 ( 1 )   107 - 110   2002.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:LUIGI PONZIO E FIGLIO  

    Matrix vesicles (MV) having high alkaline phosphatase (ALP) activity act as initiators of biological mineralization. Although bacteria have similar membranous structures to MV. ALP mediated mineralization has not been studied in bacterial cells. Escherichia coli was transformed with a bacterial ALP gene in this study. Recombinant E. coli overproducing ALP induced mineralization through hydrolysis of calcium-glycerophosphate (Ca-GP). Fourier transform infrared spectroscopy and electron microscopy combined with electron diffraction revealed newly formed hydroxyapatite mineral deposits. These findings suggest that hydrolysis of Ca-GP through ALP induced high Ca and Pi concentrations within bacterial cells followed by complete bacterial mineralization.

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  • 根管治療時の臨床症状と根管内滲出液中の起炎性サイトカイン(IL-1β)濃度との関連性 Reviewed

    大原 直子, 柳口嘉治郎, 山口慶治, 大原直子, 林 善彦

    日本歯科保存学会雑誌   45 ( 2 )   343 - 348   2002

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  • The 16-kDa alpha-crystallin-like protein of Mycobacterium bovis BCG is produced under conditions of oxygen deficiency and is associated with ribosomes Reviewed

    Y Tabira, N Ohara, N Ohara, H Kitaura, S Matsumoto, M Naito, T Yamada

    RESEARCH IN MICROBIOLOGY   149 ( 4 )   255 - 264   1998.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    A 16-kDa protein, identical to the a-crystallin-like stress protein, was induced under O-2-deficient culture conditions and bound principally to the 30S ribosomal subunits of Mycobacterium bovis BCG substrain Tokyo (BCG). The 16-kDa protein was shown to be tightly associated with the ribosome.

    DOI: 10.1016/S0923-2508(98)80301-X

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  • Characterization of the transcriptional initiation regions of genes for the major secreted protein antigens 85C and MPB51 of Mycobacterium bovis BCG Reviewed

    N Ohara, T Nishiyama, N Ohara-Wada, S Matsumoto, T Matsuo, T Yamada

    MICROBIAL PATHOGENESIS   23 ( 5 )   303 - 310   1997.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS LTD  

    The component of mycobacterial 85 complex (85A, 85B, and 85C) and MPB51 are very important from immunological, biochemical, and antimycobacterial points of view. In this study, the transcriptional properties of genes encoding three components of 85 complex and MPB51 from BCG were analysed. The authors' analyses revealed that genes for 85A and MPB51 were transcribed as a single unit despite the one operon-like structure and these four genes were probably under a different regulatory control. These findings may help to understand the immunological and physiological roles of these antigens. (C) 1997 Academic Press Limited.

    DOI: 10.1006/mpat.1997.0159

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  • Analysis of the genes encoding the antigen 85 complex and MPT51 from Mycobacterium avium Reviewed

    N Ohara, N OharaWada, H Kitaura, T Nishiyama, S Matsumoto, T Yamada

    INFECTION AND IMMUNITY   65 ( 9 )   3680 - 3685   1997.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    The components of the fibronectin-binding antigen 85 complex (85A, 85B, and 85C) and the related protein MPB/MPT51 are major secreted proteins in Mycobacterium tuberculosis and Mycobacterium bovis BCT;, The fbpA, fbpC, and mpt51 genes encoding 85A, 85C, and MPT51, respectively, were isolated from Mycobacterium avium and sequenced in this study, The structures of these genes, and that of the fbpB gene encoding the 85B protein, were conserved in these three species. The secreted amounts of 85A, 85B, 85C, and MPB/MPT51 were compared for M. tuberculosis, BCG, and M, avium, These four proteins were found in large amounts in the culture filtrates from M. tuberculosis and BCG, In contrast, in the culture filtrate from M. avium, 85B and MPT51 were abundant whereas 85A and 85C were hardly found, in spite of the presence of the encoding genes, The difference in the secretion amounts might be regulated at the transcription level, These facts might reflect host immunopathogenesis, the protective immunities against infections, and the drug susceptibilities of these organisms.

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  • HrpA, a new ribosome-associated protein which appears in heat-stressed Mycobacterium bovis bacillus Calmette-Guérin. Reviewed

    Ohara Naoko

    Journal of bacteriology   179 ( 20 )   1997

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  • Long-lasting immune response induced by recombinant Bacillus Calmette-Guerin (BCG) secretion system Reviewed

    N Wada, N Ohara, M Kameoka, Y Nishino, S Matsumoto, T Nishiyama, M Naito, H Yukitake, Y Okada, K Ikuta, T Yamada

    SCANDINAVIAN JOURNAL OF IMMUNOLOGY   43 ( 2 )   202 - 209   1996.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL SCIENCE LTD  

    The recombinant bacillus Calmette-Guerin (rBCG) secretion system utilizing an extracellular alpha antigen of Mycobacterium kansasii (alpha-K) was characterized biochemically and immunologically. The human immunodeficiency virus type1 (HIV-1)p17(gag) B cell epitope fused to alpha-K was secreted in extremely large amounts. At least three mice out of seven inoculated with rBCG generated high titres of antibody to the epitope. The long-lasting antibody production persisted more than 14 months.

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  • Stable expression and secretion of the B-cell epitope of rodent malaria from Mycobacterium bovis BCG and induction of long-lasting humoral response in mouse

    S Matsumoto, T Yanagi, N Ohara, N Wada, H Kanbara, T Yamada

    VACCINE   14 ( 1 )   54 - 60   1996.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BUTTERWORTH-HEINEMANN LTD  

    The live bacterial vaccine Mycobacterium bovis BCG (BCG) is a vehicle worth noticing for various protective antigens. The gene encoding the B-cell epitope of the oligopeptide repeating in the circumsporozoite protein (C.S. protein) of the rodent malaria parasite, Plasmodium yoelii, was inserted into the plasmid vector under the control of an expression cassette carrying the promoter and signal sequence of the a antigen derived from Mycobacterium kansasii (k-a). The B-cell epitope was successfully expressed and secreted from BCG as a fusion protein with k-a. This recombinant BCG was administered subcutaneously into BALB/c mice and the antibody production was measured by the enzyme-linked immunosorbent assay (ELISA). Long lasting humoral response was found in one of seven mice.

    DOI: 10.1016/0264-410X(95)00131-J

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  • Characterization of the gene encoding the MPB51, one of the major secreted protein antigens of Mycobacterium bovis BCG, and identification of the secreted protein closely related to the fibronectin binding 85 complex.

    Ohara Naoko

    Scandinavian Journal of Immunology   41 ( 5 )   433 - 442   1995

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  • Cloning and sequencing of the gene encoding the ribosomal L7/L12-like protein of Mycobacterium bovis BCG.

    Ohara Naoko

    Nucleic Acids Research   21 ( 15 )   3579   1993

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MISC

  • 象牙質知覚過敏抑制材がセルフエッチング接着システムの接着性に及ぼす影響 Reviewed

    松崎 久美子, 田中, 大原 直子, 澁谷 和彦, 小野 瀬里奈, 横山 章人, 山路 公造, 吉山 昌宏

    日本歯科保存学雑誌   60 ( 1 )   22 - 31   2017.2

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    Language:Japanese   Publisher:(NPO)日本歯科保存学会  

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  • Tooth Wearに対してコンポジットレジンによる審美修復を行った症例 Reviewed

    田中 久美子, 徳善 英紀, 大原 直子, 星加 知宏, 高橋 圭, 神農 泰生, 伊澤 俊次, 山路 公造, 西谷 佳浩, 吉山 昌宏

    岡山歯学会雑誌   33 ( 1 )   1 - 5   2014.6

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    Language:Japanese   Publisher:岡山歯学会  

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  • Observation of the Resin-dentin Interfacial Ultrastructure Using Confocal Laser Scanning Microscope with Fluorescent Dye Reviewed

    TANAKA Kumiko, NISHITANI Yoshihiro, OHARA Naoko, SHIBUYA Kazuhiko, YOSHIYAMA Masahiro

    The Japanese Journal of Conservative Dentistry   56 ( 4 )   310 - 317   2013

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

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  • Effects of Low-intensity Pulsed Ultrasound on Bone Formation Induced by Recombinant Human BMP-2 in Adult Rats Reviewed

    YAMAJI Kozo, SUGAYA Tsutomu, KAWANAMI Masamitsu, YOSHIYAMA Masahiro, SHIODE Shintaro, ODAJIMA Tomoomi, NISHITANI Yoshihiro, IZAWA Shunji, TANAKA Kumiko, OHARA Naoko, NISHIMURA Maiko, YOKOYAMA Akihito

    The Japanese Journal of Conservative Dentistry   56 ( 4 )   318 - 324   2013

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

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Presentations

  • 充填用コンポジットレジンの色調に関する検討

    大原 直子, 小野瀬 里奈, 松崎 久美子, 澁谷 和彦, 横山 章人, 神農 泰生, 山路 公造, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2017.5 

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    Language:Japanese  

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  • セルフアドヒーシブセメント中のMDP濃度が接着強さに及ぼす影響

    大原 直子, 田中 久美子, 澁谷 和彦, 横山 章人, 竹内 晶子, 山路 公造, 西谷 佳浩, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2015.11 

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    Language:Japanese  

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  • Actinomyces属3菌種による歯根象牙質う蝕の誘発

    大原 直子, 大原 直也, 田中 久美子, 山路 公造, 西谷 佳浩, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2014.6 

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  • 細菌バイオミネラリゼーション誘導の試み

    大原 直子, 大原 直也, 田中 久美子, 澁谷 和彦, 横山 章人, 山路 公造, 西谷 佳浩, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2013.10 

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  • 口腔連鎖球菌による歯根象牙質う蝕の誘発

    大原 直子, 田中 久美子, 澁谷 和彦, 西谷 佳浩, 大原 直也, 吉山 昌宏

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2012.10 

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Research Projects

  • surface roughness of indirect composite

    2007

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    Grant type:Competitive

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Class subject in charge

  • Clinical training: Advanced Esthetic Dentistry (2021academic year) special  - その他

  • Lecture and Research Projects: Advanced Esthetic Dentistry (2021academic year) special  - その他

  • Clinical training: Advanced Adhesive Dentistry (2021academic year) special  - その他

  • Lecture and Research Projects: Advanced Adhesive Dentistry (2021academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry I (2021academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry I (2021academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry II (2021academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry II (2021academic year) special  - その他

  • Diagnosis and treatment of Dentin/Pulp Complex (2021academic year) Fourth semester  - 金4

  • Clinical metal-based materials (2021academic year) Second semester  - 木1,木2

  • Cariology and Clinical adhesive materials (2021academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

  • Cariology and Clinical adhesive materials (2021academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

  • Clinical training: Advanced Esthetic Dentistry (2020academic year) special  - その他

  • Lecture and Research Projects: Advanced Esthetic Dentistry (2020academic year) special  - その他

  • Clinical training: Advanced Adhesive Dentistry (2020academic year) special  - その他

  • Lecture and Research Projects: Advanced Adhesive Dentistry (2020academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry I (2020academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry I (2020academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry II (2020academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry II (2020academic year) special  - その他

  • Diagnosis and treatment of Dentin/Pulp Complex (2020academic year) Fourth semester  - 金4

  • Clinical metal-based materials (2020academic year) Second semester  - 木1,木2

  • Cariology and Clinical adhesive materials (2020academic year) 1st semester  - 木1,木2

  • Cariology and Clinical adhesive materials (2020academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

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