研究者詳細 - 佐々木　孝行

論文 - 佐々木　孝行

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<scp>HalALMT1</scp> mediates malate efflux in the cortex of mature cluster rootlets of Hakea laurina, occurring naturally in severely phosphorus‐impoverished soil 査読

Hirotsuna Yamada, Lydia Ratna Bunthara, Akira Tanaka, Takuro Kohama, Hayato Maruyama, Wakana Tanaka, Sho Nishida, Tantriani, Akira Oikawa, Keitaro Tawaraya, Toshihiro Watanabe, Shu Tong Liu, Patrick M. Finnegan, Hans Lambers, Takayuki Sasaki, Jun Wasaki

New Phytologist 246 2597 - 2616 2025年2月

掲載種別：研究論文（学術雑誌）出版者・発行元：Wiley

Summary

Hakea laurina, a woody Proteaceae, naturally occurs in severely phosphorus (P)‐impoverished habitats in southwest Australia. It develops distinctive cluster roots that exhibit a high capacity for carboxylate exudation and acid phosphatase activity, contributing to its P acquisition. However, the molecular mechanisms underlying these physiological functions remain poorly understood.

We explored the cluster‐root transcriptome using de novo RNA‐Seq and identified Hakea laurina Aluminum‐activated Malate Transporter 1 (HalALMT1), encoding an aluminum (Al)‐activated malate transporter induced in mature cluster roots. We characterized HalALMT1 through electrophysiological assays and overexpression in Arabidopsis thaliana, and localized HalALMT1 expression, acid phosphatase activity, and suberized boundaries in cluster roots.

Differentially expressed genes highlighted multiple increased carboxylate‐related processes at cluster‐root maturity. HalALMT1 released malate, an activity further enhanced by exposure to Al³⁺. Notably, HalALMT1 was specifically expressed in mature cortex cells of cluster rootlets, which lack a suberized exodermis. Acid phosphatase activity was pronounced throughout the cluster rootlets, unlike in noncluster roots where it was limited to the epidermis and stele.

Substantial malate release and acid phosphatase activity in the cortex cells in cluster rootlets, which lack a suberized exodermis, allowed massive exudation. This study sheds light on an exquisite P‐acquisition strategy of Proteaceae, enabling survival under extremely low P availability.

DOI： 10.1111/nph.70010

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Functional roles of ALMT‐type anion channels in malate‐induced stomatal closure in tomato and Arabidopsis 査読

Takayuki Sasaki, Michiyo Ariyoshi, Yoko Yamamoto, Izumi C. Mori

45 2337 - 2350 2022年6月

Physiological Role of Aerobic Fermentation Constitutively Expressed in an Aluminum-Tolerant Cell Line of Tobacco (Nicotiana tabacum) 査読

Yoshiyuki Tsuchiya, Takuji Nakamura, Yohei Izumi, Keiki Okazaki, Takuro Shinano, Yasutaka Kubo, Maki Katsuhara, Takayuki Sasaki, Yoko Yamamoto

62 ( 9 ) 1460 - 1477 2021年

AtALMT3 is Involved in Malate Efflux Induced by Phosphorus Deficiency in Arabidopsis thaliana Root Hairs. 査読

Hayato Maruyama, Takayuki Sasaki, Yoko Yamamoto, Jun Wasaki

Plant & cell physiology 60 ( 1 ) 107 - 115 2019年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Under phosphorus (P)-deficient conditions, organic acid secretion from roots plays an important role in P mobilization from insoluble P in the soil. In this study, we characterized AtALMT3, a homolog of the Arabidopsis thaliana aluminum-activated malate transporter family gene. Among the 14 AtALMT family genes, only AtALMT3 was significantly up-regulated in P-deficient roots. AtALMT3 promoter::β-glucuronidase is expressed in the epidermis in roots, especially in root hair cells. AtALMT3 protein was localized in the plasma membrane and in small vesicles. Fluorescence of AtALMT3::GFP was not observed on the vacuole membrane of protoplast after lysis, indicating that AtALMT3 localizes mainly in the plasma membrane. Compared with the wild-type (WT) line, malate exudation in the AtALMT3-knockdown line (atalmt3-1) and overexpression line (atalmt3-2) under P deficiency were, respectively, 37% and 126%. In contrast, no significant difference was found in citrate exudation among these lines. The complementation of the atalmt3-1 line with AtALMT3 recovered the malate exudation to the level of the WT. Taken together, these results suggest that AtALMT3 localized in root hair membranes is involved in malate efflux in response to P deficiency.

DOI： 10.1093/pcp/pcy190

PubMed

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Aluminium-induced cell death requires upregulation of NtVPE1 gene coding vacuolar processing enzyme in tobacco (Nicotiana tabacum L.) 査読

Koki Kariya, Yoshiyuki Tsuchiya, Takayuki Sasaki, Yoko Yamamoto

Journal of Inorganic Biochemistry 181 152 - 161 2018年4月

A multidrug and toxic compound extrusion transporter mediates berberine accumulation into vacuoles in Coptis japonica 査読

Kojiro Takanashi, Yasuyuki Yamada, Takayuki Sasaki, Yoko Yamamoto, Fumihiko Sato, Kazufumi Yazaki

PHYTOCHEMISTRY 138 76 - 82 2017年6月

Overexpression of the sucrose transporter gene NtSUT1 alleviates aluminum-induced inhibition of root elongation in tobacco (Nicotiana tabacum L.) 査読

Koki Kariya, Muhammad Sameeullah, Takayuki Sasaki, Yoko Yamamoto

SOIL SCIENCE AND PLANT NUTRITION 63 ( 1 ) 45 - 54 2017年

Two Members of the Aluminum-Activated Malate Transporter Family, SlALMT4 and SlALMT5, are Expressed during Fruit Development, and the Overexpression of SlALMT5 Alters Organic Acid Contents in Seeds in Tomato (Solanum lycopersicum) 査読

Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo, Izumi C. Mori, Emi Higashiizumi, Ivan Galis, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 57 ( 11 ) 2367 - 2379 2016年11月

A Dicarboxylate Transporter, LjALMT4, Mainly Expressed in Nodules of Lotus japonicus 査読

Kojiro Takanashi, Takayuki Sasaki, Tomohiro Kan, Yuka Saida, Akifumi Sugiyama, Yoko Yamamoto, Kazufumi Yazaki

MOLECULAR PLANT-MICROBE INTERACTIONS 29 ( 7 ) 584 - 592 2016年7月

A chimeric protein of aluminum-activated malate transporter generated from wheat and Arabidopsis shows enhanced response to trivalent cations 査読

Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Peter R. Ryan, Yoko Yamamoto

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1858 ( 7 ) 1427 - 1435 2016年7月

A Domain-Based Approach for Analyzing the Function of Aluminum-Activated Malate Transporters from Wheat (Triticum aestivum) and Arabidopsis thaliana in Xenopus oocytes 査読

Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Peter R. Ryan, Takuya Furuichi, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 55 ( 12 ) 2126 - 2138 2014年12月

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.) 査読

Muhammad Sameeullah, Takayuki Sasaki, Yoko Yamamoto

SOIL SCIENCE AND PLANT NUTRITION 59 ( 5 ) 756 - 770 2013年10月

A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2 査読

Koki Kariya, Tijen Demiral, Takayuki Sasaki, Yoshiyuki Tsuchiya, Ismail Turkan, Toshio Sano, Seiichiro Hasezawa, Yoko Yamamoto

Journal of Inorganic Biochemistry 128 196 - 201 2013年

Al-induced secretion of organic acid, gene expression and root elongation in soybean roots

Daoming Wu, Man Zhao, Shouying Shen, Youqiang Fu, Takayuki Sasaki, Yoko Yamamoto, Wenhui Wei, Hong Shen

Acta Physiologiae Plantarum 35 ( 1 ) 223 - 232 2012年7月

Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K+ Channel Activity in Arabidopsis

Tahsina Sharmin Hoque, Eiji Okuma, Misugi Uraji, Takuya Furuichi, Takayuki Sasaki, Md Anamul Hoque, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 ( 3 ) 617 - 619 2012年3月

The identification of aluminium-resistance genes provides opportunities for enhancing crop production on acid soils 査読

P. R. Ryan, S. D. Tyerman, T. Sasaki, T. Furuichi, Y. Yamamoto, W. H. Zhang, E. Delhaize

JOURNAL OF EXPERIMENTAL BOTANY 62 ( 1 ) 9 - 20 2011年1月

The multiple origins of aluminium resistance in hexaploid wheat include Aegilops tauschii and more recent cis mutations to TaALMT1 査読

Peter R. Ryan, Harsh Raman, Sanjay Gupta, Takayuki Sasaki, Yoko Yamamoto, Emmanuel Delhaize

PLANT JOURNAL 64 ( 3 ) 446 - 455 2010年11月

An extracellular hydrophilic carboxy-terminal domain regulates the activity of TaALMT1, the aluminum-activated malate transport protein of wheat 査読

Takuya Furuichi, Takayuki Sasaki, Yoshiyuki Tsuchiya, Peter R. Ryan, Emmanuel Delhaize, Yoko Yamamoto

PLANT JOURNAL 64 ( 1 ) 47 - 55 2010年10月

Closing Plant Stomata Requires a Homolog of an Aluminum-Activated Malate Transporter 査読

Takayuki Sasaki, Izumi C. Mori, Takuya Furuichi, Shintaro Munemasa, Kiminori Toyooka, Ken Matsuoka, Yoshiyuki Murata, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 51 ( 3 ) 354 - 365 2010年3月

Aluminum tolerance associated with enhancement of plasma membrane H plus -ATPase in the root apex of soybean 査読

Yu-Seon Kim, Won Park, Hai Nian, Takayuki Sasaki, Bunichi Ezaki, Young-Seok Jang, Gap-Chae Chung, Hyun-Jong Bae, Sung-Ju Ahn

SOIL SCIENCE AND PLANT NUTRITION 56 ( 1 ) 140 - 149 2010年2月

Quality control of photosystem II: impact of light and heat stresses 査読

Yasusi Yamamoto, Ryota Aminaka, Miho Yoshioka, Mahbuba Khatoon, Keisuke Komayama, Daichi Takenaka, Amu Yamashita, Nobuyoshi Nijo, Kayo Inagawa, Noriko Morita, Takayuki Sasaki, Yoko Yamamoto

PHOTOSYNTHESIS RESEARCH 98 ( 1-3 ) 589 - 608 2008年10月

Characterization of the TaALMT1 protein as an Al(3+)-activated anion channel in transformed tobacco (Nicotiana tabacum L.) cells 査読

Wen-Hao Zhang, Peter R. Ryan, Takayuki Sasaki, Yoko Yamamoto, Wendy Sullivan, Steve D. Tyerman

PLANT AND CELL PHYSIOLOGY 49 ( 9 ) 1316 - 1330 2008年9月

Analysis of TaALMT1 traces the transmission of aluminum resistance in cultivated common wheat (Triticum aestivum L.)

Harsh Raman, Peter R. Ryan, Rosy Raman, Benjamin J. Stodart, Kerong Zhang, Peter Martin, Rachel Wood, Takayuki Sasaki, Yoko Yamamoto, Michael Mackay, Diane M. Hebb, Emmanuel Delhaize

THEORETICAL AND APPLIED GENETICS 116 ( 3 ) 343 - 354 2008年2月

The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum) 査読

Hirotoshi Motoda, Takayuki Sasaki, Yoshio Kano, Peter R. Ryan, Emmanuel Delhaize, Hideaki Matsumoto, Yoko Yamamoto

Plant Signaling and Behavior 2 ( 6 ) 467 - 472 2007年

Sequence upstream of the wheat (Triticum aestivum L.) ALMT1 gene and its relationship to aluminum resistance 査読

Takayuki Sasaki, Peter R. Ryan, Emmanuel Delhaize, Diane M. Hebb, Yasunari Ogihara, Kanako Kawaura, Kazuhiro Noda, Toshio Kojima, Atsushi Toyoda, Hideaki Matsumoto, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 47 ( 10 ) 1343 - 1354 2006年10月

AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis 査読

OA Hoekenga, LG Maron, MA Pineros, GMA Cancado, J Shaff, Y Kobayashi, PR Ryan, B Dong, E Delhaize, T Sasaki, H Matsumoto, Y Yamamoto, H Koyama, LV Kochian

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 103 ( 25 ) 9738 - 9743 2006年6月

Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation 査読

H Shen, JH Chen, ZY Wang, CY Yang, T Sasaki, Y Yamamoto, H Matsumoto, XL Yan

JOURNAL OF EXPERIMENTAL BOTANY 57 ( 6 ) 1353 - 1362 2006年3月

Molecular characterization and mapping of ALMT1, the aluminium-tolerance gene of bread wheat (Triticum aestivum L.) 査読

H Raman, KR Zhang, M Cakir, R Appels, DF Garvin, LG Maron, LV Kochian, JS Moroni, R Raman, M Imtiaz, F Drake-Brockman, Waters, I, P Martin, T Sasaki, Y Yamamoto, H Matsumoto, DM Hebb, E Delhaize, PR Ryan

GENOME 48 ( 5 ) 781 - 791 2005年10月

Citrate secretion coupled with the modulation of soybean root tip under aluminum stress. Up-regulation of transcription, translation, and threonine-oriented phosphorylation of plasma membrane H+-ATPase (vol 138, pg 287, 2005) 査読

H Shen, LF He, T Sasaki, Y Yamamoto, SJ Zheng, A Ligaba, XL Yan, SJ Ahn, M Yamaguchi, H Sasakawa, H Matsumoto

PLANT PHYSIOLOGY 139 ( 1 ) 557 - 557 2005年9月

Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination 査読

S Kikui, T Sasaki, M Maekawa, A Miyao, H Hirochika, H Matsumoto, Y Yamamoto

JOURNAL OF INORGANIC BIOCHEMISTRY 99 ( 9 ) 1837 - 1844 2005年9月

Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1) 査読

M Yamaguchi, T Sasaki, M Sivaguru, Y Yamamoto, H Osawa, SJ Ahn, H Matsumoto

PLANT AND CELL PHYSIOLOGY 46 ( 5 ) 812 - 816 2005年5月

Engineering high-level aluminum tolerance in barley with the ALMT1 gene 査読

E Delhaize, PR Ryan, DM Hebb, Y Yamamoto, T Sasaki, H Matsumoto

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101 ( 42 ) 15249 - 15254 2004年10月

A wheat gene encoding an aluminum-activated malate transporter 査読

T Sasaki, Y Yamamoto, B Ezaki, M Katsuhara, SJ Ahn, PR Ryan, E Delhaize, H Matsumoto

PLANT JOURNAL 37 ( 5 ) 645 - 653 2004年3月

A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat 査読

T Sasaki, B Ezaki, H Matsumoto

PLANT AND CELL PHYSIOLOGY 43 ( 2 ) 177 - 185 2002年2月

Development of vacuoles and vacuolar H+-ATPase activity under extremely high CO2 conditions in Chlorococcum littorale cells 査読

T Sasaki, NA Pronina, M Maeshima, Iwasaki, I, N Kurano, S Miyachi

PLANT BIOLOGY 1 ( 1 ) 68 - 75 1999年1月

Induction of ferric reductase activity and of iron uptake capacity in Chlorococcum littorale cells under extremely high-CO2 and iron-deficient conditions 査読

T Sasaki, N Kurano, S Miyachi

PLANT AND CELL PHYSIOLOGY 39 ( 4 ) 405 - 410 1998年4月

Cloning and characterization of High-CO2-specific cDNAs from a marine microalga, Chlorococcum littorale, and effect of CO2 concentration and iron deficiency on the gene expression 査読

T Sasaki, N Kurano, S Miyachi

PLANT AND CELL PHYSIOLOGY 39 ( 2 ) 131 - 138 1998年2月

Carbonic anhydrase is induced prior to arylsulfatase under the simultaneous deprivation of inorganic carbon and sulfate in Chlamydomonas reinhardtii (Volvocales, Chlorophyta) 査読

Takayuki Sasaki, Robert K. Togasaki, Yoshihiro Shiraiwa

Phycological Research 45 ( 4 ) 207 - 211 1997年

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