研究者詳細 - 佐々木　孝行

論文 - 佐々木　孝行

分割表示 38 件中 1 - 20 件目／全件表示 >>

<scp>HalALMT1</scp> mediates malate efflux in the cortex of mature cluster rootlets of Hakea laurina, occurring naturally in severely phosphorus‐impoverished soil 査読

Hirotsuna Yamada, Lydia Ratna Bunthara, Akira Tanaka, Takuro Kohama, Hayato Maruyama, Wakana Tanaka, Sho Nishida, Tantriani, Akira Oikawa, Keitaro Tawaraya, Toshihiro Watanabe, Shu Tong Liu, Patrick M. Finnegan, Hans Lambers, Takayuki Sasaki, Jun Wasaki

New Phytologist 246 2597 - 2616 2025年2月

掲載種別：研究論文（学術雑誌）出版者・発行元：Wiley

Summary

Hakea laurina, a woody Proteaceae, naturally occurs in severely phosphorus (P)‐impoverished habitats in southwest Australia. It develops distinctive cluster roots that exhibit a high capacity for carboxylate exudation and acid phosphatase activity, contributing to its P acquisition. However, the molecular mechanisms underlying these physiological functions remain poorly understood.

We explored the cluster‐root transcriptome using de novo RNA‐Seq and identified Hakea laurina Aluminum‐activated Malate Transporter 1 (HalALMT1), encoding an aluminum (Al)‐activated malate transporter induced in mature cluster roots. We characterized HalALMT1 through electrophysiological assays and overexpression in Arabidopsis thaliana, and localized HalALMT1 expression, acid phosphatase activity, and suberized boundaries in cluster roots.

Differentially expressed genes highlighted multiple increased carboxylate‐related processes at cluster‐root maturity. HalALMT1 released malate, an activity further enhanced by exposure to Al³⁺. Notably, HalALMT1 was specifically expressed in mature cortex cells of cluster rootlets, which lack a suberized exodermis. Acid phosphatase activity was pronounced throughout the cluster rootlets, unlike in noncluster roots where it was limited to the epidermis and stele.

Substantial malate release and acid phosphatase activity in the cortex cells in cluster rootlets, which lack a suberized exodermis, allowed massive exudation. This study sheds light on an exquisite P‐acquisition strategy of Proteaceae, enabling survival under extremely low P availability.

DOI： 10.1111/nph.70010

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Functional roles of ALMT‐type anion channels in malate‐induced stomatal closure in tomato and Arabidopsis 査読

Takayuki Sasaki, Michiyo Ariyoshi, Yoko Yamamoto, Izumi C. Mori

45 2337 - 2350 2022年6月

Physiological Role of Aerobic Fermentation Constitutively Expressed in an Aluminum-Tolerant Cell Line of Tobacco (Nicotiana tabacum) 査読

Yoshiyuki Tsuchiya, Takuji Nakamura, Yohei Izumi, Keiki Okazaki, Takuro Shinano, Yasutaka Kubo, Maki Katsuhara, Takayuki Sasaki, Yoko Yamamoto

62 ( 9 ) 1460 - 1477 2021年

AtALMT3 is Involved in Malate Efflux Induced by Phosphorus Deficiency in Arabidopsis thaliana Root Hairs. 査読

Hayato Maruyama, Takayuki Sasaki, Yoko Yamamoto, Jun Wasaki

Plant & cell physiology 60 ( 1 ) 107 - 115 2019年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Under phosphorus (P)-deficient conditions, organic acid secretion from roots plays an important role in P mobilization from insoluble P in the soil. In this study, we characterized AtALMT3, a homolog of the Arabidopsis thaliana aluminum-activated malate transporter family gene. Among the 14 AtALMT family genes, only AtALMT3 was significantly up-regulated in P-deficient roots. AtALMT3 promoter::β-glucuronidase is expressed in the epidermis in roots, especially in root hair cells. AtALMT3 protein was localized in the plasma membrane and in small vesicles. Fluorescence of AtALMT3::GFP was not observed on the vacuole membrane of protoplast after lysis, indicating that AtALMT3 localizes mainly in the plasma membrane. Compared with the wild-type (WT) line, malate exudation in the AtALMT3-knockdown line (atalmt3-1) and overexpression line (atalmt3-2) under P deficiency were, respectively, 37% and 126%. In contrast, no significant difference was found in citrate exudation among these lines. The complementation of the atalmt3-1 line with AtALMT3 recovered the malate exudation to the level of the WT. Taken together, these results suggest that AtALMT3 localized in root hair membranes is involved in malate efflux in response to P deficiency.

DOI： 10.1093/pcp/pcy190

PubMed

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Aluminium-induced cell death requires upregulation of NtVPE1 gene coding vacuolar processing enzyme in tobacco (Nicotiana tabacum L.) 査読

Koki Kariya, Yoshiyuki Tsuchiya, Takayuki Sasaki, Yoko Yamamoto

Journal of Inorganic Biochemistry 181 152 - 161 2018年4月

A multidrug and toxic compound extrusion transporter mediates berberine accumulation into vacuoles in Coptis japonica 査読

Kojiro Takanashi, Yasuyuki Yamada, Takayuki Sasaki, Yoko Yamamoto, Fumihiko Sato, Kazufumi Yazaki

PHYTOCHEMISTRY 138 76 - 82 2017年6月

Overexpression of the sucrose transporter gene NtSUT1 alleviates aluminum-induced inhibition of root elongation in tobacco (Nicotiana tabacum L.) 査読

Koki Kariya, Muhammad Sameeullah, Takayuki Sasaki, Yoko Yamamoto

SOIL SCIENCE AND PLANT NUTRITION 63 ( 1 ) 45 - 54 2017年

Two Members of the Aluminum-Activated Malate Transporter Family, SlALMT4 and SlALMT5, are Expressed during Fruit Development, and the Overexpression of SlALMT5 Alters Organic Acid Contents in Seeds in Tomato (Solanum lycopersicum) 査読

Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo, Izumi C. Mori, Emi Higashiizumi, Ivan Galis, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 57 ( 11 ) 2367 - 2379 2016年11月

A Dicarboxylate Transporter, LjALMT4, Mainly Expressed in Nodules of Lotus japonicus 査読

Kojiro Takanashi, Takayuki Sasaki, Tomohiro Kan, Yuka Saida, Akifumi Sugiyama, Yoko Yamamoto, Kazufumi Yazaki

MOLECULAR PLANT-MICROBE INTERACTIONS 29 ( 7 ) 584 - 592 2016年7月

A chimeric protein of aluminum-activated malate transporter generated from wheat and Arabidopsis shows enhanced response to trivalent cations 査読

Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Peter R. Ryan, Yoko Yamamoto

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1858 ( 7 ) 1427 - 1435 2016年7月

A Domain-Based Approach for Analyzing the Function of Aluminum-Activated Malate Transporters from Wheat (Triticum aestivum) and Arabidopsis thaliana in Xenopus oocytes 査読

Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Peter R. Ryan, Takuya Furuichi, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 55 ( 12 ) 2126 - 2138 2014年12月

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.) 査読

Muhammad Sameeullah, Takayuki Sasaki, Yoko Yamamoto

SOIL SCIENCE AND PLANT NUTRITION 59 ( 5 ) 756 - 770 2013年10月

A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2 査読

Koki Kariya, Tijen Demiral, Takayuki Sasaki, Yoshiyuki Tsuchiya, Ismail Turkan, Toshio Sano, Seiichiro Hasezawa, Yoko Yamamoto

Journal of Inorganic Biochemistry 128 196 - 201 2013年

Al-induced secretion of organic acid, gene expression and root elongation in soybean roots

Daoming Wu, Man Zhao, Shouying Shen, Youqiang Fu, Takayuki Sasaki, Yoko Yamamoto, Wenhui Wei, Hong Shen

Acta Physiologiae Plantarum 35 ( 1 ) 223 - 232 2012年7月

Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K+ Channel Activity in Arabidopsis

Tahsina Sharmin Hoque, Eiji Okuma, Misugi Uraji, Takuya Furuichi, Takayuki Sasaki, Md Anamul Hoque, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 ( 3 ) 617 - 619 2012年3月

The identification of aluminium-resistance genes provides opportunities for enhancing crop production on acid soils 査読

P. R. Ryan, S. D. Tyerman, T. Sasaki, T. Furuichi, Y. Yamamoto, W. H. Zhang, E. Delhaize

JOURNAL OF EXPERIMENTAL BOTANY 62 ( 1 ) 9 - 20 2011年1月

The multiple origins of aluminium resistance in hexaploid wheat include Aegilops tauschii and more recent cis mutations to TaALMT1 査読

Peter R. Ryan, Harsh Raman, Sanjay Gupta, Takayuki Sasaki, Yoko Yamamoto, Emmanuel Delhaize

PLANT JOURNAL 64 ( 3 ) 446 - 455 2010年11月

An extracellular hydrophilic carboxy-terminal domain regulates the activity of TaALMT1, the aluminum-activated malate transport protein of wheat 査読

Takuya Furuichi, Takayuki Sasaki, Yoshiyuki Tsuchiya, Peter R. Ryan, Emmanuel Delhaize, Yoko Yamamoto

PLANT JOURNAL 64 ( 1 ) 47 - 55 2010年10月

Closing Plant Stomata Requires a Homolog of an Aluminum-Activated Malate Transporter 査読

Takayuki Sasaki, Izumi C. Mori, Takuya Furuichi, Shintaro Munemasa, Kiminori Toyooka, Ken Matsuoka, Yoshiyuki Murata, Yoko Yamamoto

PLANT AND CELL PHYSIOLOGY 51 ( 3 ) 354 - 365 2010年3月

Aluminum tolerance associated with enhancement of plasma membrane H plus -ATPase in the root apex of soybean 査読

Yu-Seon Kim, Won Park, Hai Nian, Takayuki Sasaki, Bunichi Ezaki, Young-Seok Jang, Gap-Chae Chung, Hyun-Jong Bae, Sung-Ju Ahn

SOIL SCIENCE AND PLANT NUTRITION 56 ( 1 ) 140 - 149 2010年2月

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