Faculty Profiles - NISHINO Naoki

Papers - NISHINO Naoki

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Cyclic oligosaccharide-induced modulation of immunoglobulin A reactivity to gut bacteria contributes to alterations in the bacterial community structure

Miyamoto, T., Tsuruta, T., Teraoka, M., Wang, T., Nishino, N.

Nutrients 16 2824 - 2824 2024.8

Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Immunoglobulin A (IgA) is a major gut antibody that coats commensal gut bacteria and contributes to shaping a stable gut bacterial composition. Although previous studies have shown that cyclic oligosaccharides, including cyclic nigerosyl-1,6-nigerose (CNN) and cyclodextrins (CDs, including αCD, βCD, and γCD), alter the gut bacterial composition, it remains unclear whether cyclic oligosaccharides modify the IgA coating of gut bacteria, which relates to cyclic oligosaccharide-induced alteration of the gut bacterial composition. To address this issue, mice were maintained for 12 weeks on diets containing CNN, αCD, βCD, or γCD; the animals’ feces were evaluated for their bacterial composition and the IgA coating index (ICI), a measure of the degree of IgA coating of bacteria. We observed that the intake of each cyclic oligosaccharide altered the gut bacterial composition, with changes in the ICI found at both the phylum and genus levels. The ICI for Bacillota, Lachnospiraceae NK4A136 group, UC Lachnospiraceae, and Tuzzerella were significantly and positively correlated with the relative abundance (RA) in total bacteria for these bacteria; in contrast, significant correlations were not seen for other phyla and genera. Our observations suggest that cyclic oligosaccharide-induced modulation of the IgA coating of gut bacteria may partly relate to changes in the community structure of the gut bacteria.

DOI： 10.3390/nu16172824

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Unabsorbed fecal fat content correlates with a reduction of immunoglobulin A coating of gut bacteria in high-lard diet-fed mice. International journal

Katsumata, E., Tsuruta, T., Sonoyama, K., Yoshida, T., Sasaki, M., Teraoka, M., Wang, T., Nishino, N.

Molecular Nutrition & Food Research 68 e2400078 2024.8

Language：English Publishing type：Research paper (scientific journal)

SCOPE: Immunoglobulin A (IgA) selectively coats gut bacteria and contributes to regulatory functions in gastrointestinal inflammation and glucose metabolism. Excess intake of lard leads to decrease in the IgA coating of gut bacteria, although the underlying mechanisms remain unknown. This study validates how unabsorbed fat derived from a high-lard diet in the gut affects the IgA coating of bacteria, as assessed in mouse models using three types of dietary fat (lard, medium-, and long-chain triglycerides [MLCTs], and medium-chain triglycerides [MCTs]) exhibiting different digestibilities. METHODS AND RESULTS: C57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA-coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard-fed mice, MLCT- and MCT-fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations. CONCLUSION: Unabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high-lard diet-fed mice.

DOI： 10.1002/mnfr.202400078

PubMed

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Variations in milk, udder skin, and fecal microbiota and their relationships with blood metabolites and milk composition in dairy cows. Reviewed

AoDaohu, Akada, K., Gathinji, P.K., Nishino, N.

Letters in Applied Microbiology 77 ovae014 2024.3

Commercial concentrate supplementation in Phan Rang sheep diets: Effects on digestibility traits, growth and carcass performance. Reviewed

Nguyen, H.V., Nguyen, T.M., Dinh, V.D., Van, N.P., Tran, N.L., Le, T.H., Le, D.T., Vo, T.M., Ngo, M.D., Bui, V.L., Nguyen, X.B., Ton, N.M.T., Nishino, N.

Advances of Animal and Veterinary Sciences 12 1 - 8 2024.1

In vitro assessment of the relationships between the digestion of different types of rice straw and bacterial community in the rumen. Reviewed

68 372 - 382 2023.9

Cyclic nigerosylnigerose attenuates high-fat diet induced fa;dposition;colonic inflammation;d;abnormal;glucose metabolism;modifies gut immunoglobulin A reactivity to commensal bacteria. Reviewed International journal

Tsuruta, T, Sonoyama, K, Miyamoto, T, Nguyen, Q.D, Mizote, A, Sasou, H, Shiotsu, M, Teraoka, M, Nishino, N

Molecular Nutrition and Food Research 67 202200389 2023.7

Language：English Publishing type：Research paper (scientific journal)

SCOPE: High-fat diet (HFD) intake induces gut dysbiosis, inflammation in the peripheral tissues, and a reduction in immunoglobulin A (IgA) coating of gut bacteria, which is related to HFD-induced insulin resistance (IR). This study evaluates the effect of cyclic nigerosylnigerose (CNN), a dietary fiber that prevents gut inflammation and promotes IgA coating of gut bacteria, on the above-mentioned HFD-induced disorders. METHODS AND RESULTS: Balb/c mice are fed an HFD and administered CNN for 20 weeks. CNN administration reduces mesenteric adipose tissue weight, colonic tumor necrosis factor α (TNFα) mRNA expression, and serum endotoxin levels and ameliorates HFD-induced abnormal glucose metabolism. Additionally, CNN administration promotes gut bacteria-specific IgA secretion and alters IgA reactivity to gut bacteria. The alterations of IgA reactivity to specific bacteria such as Erysipelatoclostridium, Escherichia, Faecalibaculum, Lachnospiraceae genera, and Stenotrophomonas are correlated with mesenteric adipose tissue weight, colonic TNFα mRNA expression, serum endotoxin levels, and a homeostasis model assessment for IR. CONCLUSION: CNN-induced alterations in IgA reactivity to gut bacteria may be related to the suppression of HFD-induced fat deposition, colonic inflammation, endotoxemia, and IR. These observations indicate that dietary fiber that modulates IgA reactivity to gut bacteria may be useful in preventing HFD-induced disorders.

DOI： 10.1002/mnfr.202200389

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Modulatory effects of A1 milk, A2 milk, soy, and egg proteins on gut microbiota and fermentation. Reviewed

Nuomin, Baek, R, Tsuruta, T, Nishino, N

Microorganisms 11 1194 2023.5

Monitoring the milk composition, milk microbiota, and blood metabolites of Jersey cows throughout a lactation period. Reviewed

Gathinji, P.K., Yousofi, Z., Akada, K., Wali, A., Nishino, N.

Veterinary Sciences 10 226 2023.3

Frequency of β-casein gene polymorphisms in Jersey cows in western Japan. Reviewed

Nuomin, Nguyen, D.Q, Aodaohu, Nishino, N

Animals 12 2076 2022.8

Bacterial and fungal microbiota of total mixed ration silage stored at various temperatures. Reviewed International journal

Wali, A, Hou, J, Tsuruta, T, Nishino, N

Journal of Applied Microbiology 133 ( 2 ) 579 - 590 2022.8

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

AIMS: To obtain insights into how bacterial and fungal microbiota and fermentation products composition are affected by storage temperature for TMR silage, which can be manufactured year-round. METHODS AND RESULTS: TMR silage was stored at 10°C, 25°C, ambient temperature (AT; 20-35°C) and 40°C. Lactic acid production was delayed when stored at 10°C, and acid production stagnated after 2 weeks when stored at 40°C. The patterns of acetic acid and ethanol production were inversely related, with ethanol production promoted at 10°C and 25°C and acetic acid production promoted at AT and 40°C. The bacterial diversity was reduced in TMR silage with high lactic acid and acetic acid content, and the fungal diversity was reduced in TMR silage with high ethanol content. CONCLUSIONS: The intensity of lactic acid production was accounted for by the high abundance of Lactobacillus, and its stagnated production at a substantially high storage temperature was related to an increased abundance of Bacillus. The enhanced production of acetic acid or ethanol can be explained by differences in the fungal microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY: The integrated analysis of bacterial and fungal microbiota can provide in-depth insights into the impact of storage temperature on TMR silage fermentation.

DOI： 10.1111/jam.15582

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Fecal, milk, uterine, airborne dust, and water microbiota in dairy farms in southern Vietnam: A pilot study. Reviewed

Tran, T.M.T, Tran, H.D, Nguyen, T.T, Acosta, T.J, Tsuruta, T, Nishino, N, Duong, T.H

Advances of Animal and Veterinary Sciences 10 ( 7 ) 1525 - 1531 2022.7

Publishing type：Research paper (scientific journal)

Six sets of fecal, milk, and uterine samples from dairy cows and two sets of airborne dust and water samples from the cowshed were collected from two farms in Ho Chi Minh City at 20–40 days postpartum. The microbiota was characterized using Illumina sequencing of the V4 hypervariable region of the 16S rRNA genes. The predominant species in the fecal microbiota was not as predominant in the milk microbiota, but occasionally appeared in the uterine microbiota. The microbiota from the airborne dust and water samples did not show a relation with either the milk or the uterine microbiota, whereas Moraxellaceae, the most abundant family in the airborne dust and water microbiota, was detected at low proportions in the milk microbiota. The farm-to-farm difference was more apparent for the milk microbiota than for the fecal and uterine microbiota. It is noteworthy that Dermacoccaceae, Bacillaceae, and Methylobacteriaceae were the prevalent families in the microbiota of several milk and uterine samples; however, these findings did not help understand the role of the microbiota in fertility and subsequent conception. Despite the small number of dairy cows examined in this study, this report is the first to characterize the fecal, milk, uterine, airborne dust, and water microbiota in dairy farms in southern Vietnam.

DOI： 10.17582/journal.aavs/2022/10.7.1525.1531

Scopus

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Bacterial and fungal microbiota of guinea grass silage shows various levels of acetic acid fermentation Reviewed

Hou, J, Nishino, N

Fermentation 8 10 2022.1

Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice Reviewed

Tsuruta, T, Muhomah, T.A, Sonoyama, K, Nguyen, Q.D, Takase, Y, Nishijima, A, Himoto, S, Katsumata, E, Nishino, N

Nutrition Research 93 15 - 26 2021.9

Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides Reviewed International journal

Sivixay, S, Bai, G, Tsuruta, T, Nishino, N

AIMS Microbiology 7 ( 1 ) 1 - 12 2021.1

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Diet is considered the most influential factor in modulating the gut microbiota but how dietary protein sources differ in their modulatory effects is not well understood. In this study, soy, meat (mixture of beef and pork), and fish proteins (experiment 1) and soy, milk (casein), and egg proteins (experiment 2) were fed to rats with cellulose (CEL) and raffinose (RAF); the microbiota composition and short-chain fatty acid concentration in the cecum were determined. Egg protein feeding decreased the concentration of acetic acid and the richness and diversity of the cecum microbiota. RAF feeding increased the concentrations of acetic and propionic acids and decreased the richness and diversity of the cecum microbiota. When fed with CEL, the abundance of Ruminococcaceae and Christensenellaceae, Akkermansiaceae and Tannerellaceae, and Erysipelotrichaceae enhanced with soy protein, meat and fish proteins, and egg protein, respectively. The effects of dietary proteins diminished with RAF feeding and the abundance of Bifidobacteriaceae, Erysipelotrichaceae, and Lachnospiraceae increased and that of Ruminococcaceae and Christensenellaceae decreased regardless of the protein source. These results indicate that, although the effect of prebiotics is more robust and distinctive, dietary protein sources may influence the composition and metabolic activities of the gut microbiota. The stimulatory effects of soy, meat, and egg proteins on Christensenellaceae, Akkermansiaceae, and Erysipelotrichaceae deserve further examination to better elucidate the dietary manipulation of the gut microbiota.

DOI： 10.3934/microbiol.2021001

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An investigation of seasonal variations in the microbiota of milk, feces, bedding, and airborne dust Reviewed

Nguyen, T.T, Wu, H, Nishino, N

Asian-Australasian Journal of Animal Sciences 33 ( 11 ) 1858 - 1865 2020.11

Bacterial and fungal microbiota associated with the ensiling of wet soybean curd residue under prompt and delayed sealing conditions Reviewed

Wali, A, Nishino, N

Microorganisms 8 9 2020.9

Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice Reviewed

Tsuruta, T, Katsumata, E, Mizote, A, Hou, J, Muhomah, T.A, Nishino, N

Bioscience of Microbiota, Food and Health 39 ( 3 ) 188 - 196 2020.7

Language：English Publishing type：Research paper (scientific journal)

Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.

DOI： 10.12938/bmfh.2020-012

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Examination of milk microbiota, fecal microbiota, and blood metabolites of Jersey cows in cool and hot seasons Reviewed International journal

Nguyen, Q.D, Tsuruta, T, Nishino, N

Animal Science Journal 91 ( 1 ) e13441 2020.1

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Microbiota of individual cow milk, bulk tank milk, and feces of Jersey cows were examined. Samples were collected from two farms (F1 and F2) in cool (November, Nov) and hot (July, Jul) seasons. Milk yield and milk composition were similar between the two farms and between the two seasons. Prevalent taxa of the fecal microbiota, i.e. Ruminococcaceae, Bacteroidaceae, Lachnospiraceae, Rikenellaceae, and Clostridiaceae, were unaffected by the farm and season. Relative abundance of milk microbiota for Pseudomonadaceae, Enterobacteriaceae, and Streptococcaceae (F1 > F2) and Lactobacillaceae, Bifidobacteriaceae, and Cellulomonadaceae (F1 < F2) were different between the two farms, and those for Staphylococcaceae, Bacillaceae, Ruminococcaceae, and Veillonellaceae (Nov < Jul) and Methylobacteriaceae and Moraxellaceae (Nov > Jul) were different between the two seasons. The microbiota of bulk tank milk was numerically different from that of individual cow milk. Principal coordinate analysis indicated that the milk microbiota was unrelated to the fecal microbiota. The finding that relative abundance of Pseudomonadaceae and Moraxellaceae appeared greater than those reported for Holstein milk suggested that higher protein and fat content may result in a greater abundance of proteolytic and lipolytic taxa in Jersey cow milk.

DOI： 10.1111/asj.13441

PubMed

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The relationship between uterine, fecal, bedding, and airborne dust microbiota from dairy cows and their environment: A pilot study Reviewed

Nguyen, T.T, Miyake, A, Tran, T.T.M, Tsuruta, T, Nishino, N

Animals 9 ( 12 ) 1007 2019.12

High-fat diet reduces the level of secretory immunoglobulin A coating of commensal gut microbiota Reviewed

Muhomah, T.A, Nishino, N, Katsumata, E, Wu, H, Tsuruta, T

Bioscience of Microbiota, Food and Health 38 ( 2 ) 55 - 64 2019.4

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