2022/01/04 更新

写真a

ニシノ ナオキ
西野 直樹
NISHINO Naoki
所属
環境生命科学学域 教授
職名
教授
外部リンク

学位

  • 博士(農学) ( 名古屋大学 )

研究キーワード

  • 動物栄養学

  • 動物衛生学

  • 野生動物学

  • 飼料学

研究分野

  • ライフサイエンス / 動物生産科学

学歴

  • 名古屋大学    

    - 1990年

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    国名: 日本国

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  • 名古屋大学   School of Agricultural Sciences  

    - 1988年

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    国名: 日本国

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経歴

  • 岡山大学環境生命科学研究科 教授

    2015年

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  • 岡山大学自然科学研究科 准教授

    2004年 - 2014年

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所属学協会

委員歴

  • 岡山県農林水産総合センター畜産研究所評価委員会   評価委員  

    2021年4月 - 2022年3月   

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    団体区分:自治体

 

論文

  • Bacterial and fungal microbiota of guinea grass silage shows various levels of acetic acid fermentation 査読

    Hou, J, Nishino, N

    Fermentation   8   10   2022年1月

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    担当区分:責任著者  

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  • Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice 査読

    Tsuruta, T, Muhomah, T.A, Sonoyama, K, Nguyen, Q.D, Takase, Y, Nishijima, A, Himoto, S, Katsumata, E, Nishino, N

    Nutrition Research   93   15 - 26   2021年9月

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  • Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides 査読

    Sivixay, S, Bai, G, Tsuruta, T, Nishino, N

    AIMS Microbiology   7   1 - 12   2021年1月

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    担当区分:責任著者  

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  • An investigation of seasonal variations in the microbiota of milk, feces, bedding, and airborne dust 査読

    Nguyen, T.T, Wu, H, Nishino, N

    Asian-Australasian Journal of Animal Sciences   33 ( 11 )   1858 - 1865   2020年11月

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    担当区分:責任著者  

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  • Bacterial and fungal microbiota associated with the ensiling of wet soybean curd residue under prompt and delayed sealing conditions 査読

    Wali, A, Nishino, N

    Microorganisms   8   9   2020年9月

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    担当区分:責任著者  

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  • Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice 査読

    Tsuruta, T, Katsumata, E, Mizote, A, Hou, J, Muhomah, T.A, Nishino, N

    Bioscience of Microbiota, Food and Health   39 ( 3 )   188 - 196   2020年7月

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  • Examination of milk microbiota, fecal microbiota, and blood metabolites of Jersey cows in cool and hot seasons 査読

    Nguyen, Q.D, Tsuruta, T, Nishino, N

    Animal Science Journal   91   e13441   2020年1月

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    担当区分:責任著者  

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  • The relationship between uterine, fecal, bedding, and airborne dust microbiota from dairy cows and their environment: A pilot study 査読

    Nguyen, T.T, Miyake, A, Tran, T.T.M, Tsuruta, T, Nishino, N

    Animals   9 ( 12 )   1007   2019年12月

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    担当区分:責任著者  

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  • High-fat diet reduces the level of secretory immunoglobulin A coating of commensal gut microbiota 査読

    Muhomah, T.A, Nishino, N, Katsumata, E, Wu, H, Tsuruta, T

    Bioscience of Microbiota, Food and Health   38 ( 2 )   55 - 64   2019年4月

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  • Rumen fluid, feces, milk, water, feed, airborne dust, and bedding microbiota in dairy farms managed by automatic milking systems 査読

    Wu, H, Nguyen, Q.D, Tran, T.T.M, Tang, M.T, Tsuruta, T, Nishino, N

    Animal Science Journal   90 ( 3 )   445 - 452   2019年3月

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    担当区分:責任著者  

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  • Dietary soy, meat, and fish proteins modulate the effects of prebiotic raffinose on composition and fermentation of gut microbiota in rats 査読

    Gaowa Bai, Takeshi Tsuruta, Naoki Nishino

    International Journal of Food Sciences and Nutrition   69 ( 4 )   480 - 487   2018年5月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Taylor and Francis Ltd  

    Soy, meat (mixture of pork and beef), and fish proteins were fed to rats with and without prebiotic raffinose (RAF), and the composition and fermentation of gut microbiota were examined. Bifidobacterium spp. populations were higher, and propionic acid concentration was lower in soy protein-fed than meat protein-fed rats. Likewise, Enterobacteriaceae populations were higher in fish protein-fed rats than other rats. RAF feeding increased Bifidobacterium spp. and decreased Faecalibacterium prausnitzii populations regardless of the dietary protein source. Interactions between dietary proteins and RAF were shown for Lactobacillus spp. and Clostridium perfringens group
    the increase of Lactobacillus spp. populations by RAF was seen only for soy protein-fed rats, whereas the reduction of C. perfringens group by RAF was evident in fish and meat protein-fed rats. It is concluded that dietary proteins may differentially modulate the effects of prebiotic oligosaccharides on gut fermentation and microbiota, with differences observed between plant and animal proteins.

    DOI: 10.1080/09637486.2017.1382454

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  • Effects of wilting and lactic acid bacteria inoculation on fermentation and microbial community of elephant grass silage produced in Vietnam 査読

    Thi Minh Tu Tran, Mui Thi Nguyen, Huu Van Nguyen, Naoki Nishino

    Grassland Science   64 ( 2 )   151 - 155   2018年4月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Inc.  

    Direct-cut (dry matter [DM] 165 g kg−1) and wilted (DM 250 g kg−1) elephant grass silages were prepared in Hue, Vietnam, with and without a mixture of Lactobacillus paracasei and Lactococcus lactis (LP+LC) or Lactobacillus buchneri (LB). The silages were stored for 4 months, and fermentation products and aerobic stability were determined. The microbial community was assessed using denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Acetate was predominantly produced during the fermentation of control and LB-inoculated silages, whereas the content was not sufficient to inhibit aerobic spoilage for 7 days in control silage. The LP+LC inoculation greatly enhanced lactate content, suppressed the alcohol content, and did not improve aerobic stability or decrease the total fungal population. The bacterial community and L. buchneri populations were similar between control and LB-inoculated silages
    however, Kluyveromyces delphensis was undetectable and the total fungal population was lowered in LB-inoculated silage.

    DOI: 10.1111/grs.12187

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  • Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut 査読

    Hongyan Han, Chao Wang, Yanbing Li, Zhu Yu, Qingfang Xu, Guangpeng Li, Tang Thuy Minh, Naoki Nishino

    Animal Science Journal   89 ( 1 )   97 - 104   2018年1月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing  

    In order to assess the survival of lactic acid bacteria (LAB) in whole crop maize silage in the gut of dairy cows, one representative silage sample and three different feces samples were collected from dairy cows on three dairy farms in Hua Bei, China and three dairy farms in Kyushu, Japan. The composition of the bacterial community was examined by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Lactobacillus acetotolerans was detected in all bunker-made maize silage samples, regardless of the dairy farm or sampling region from which they were sourced. A total of eight LAB species were detected in the maize silage samples, of which three (L. acetotolerans, L. pontis and L. casei) appeared to survive digestion. The populations of L. acetotolerans in silage and feces were 106–7 and 103–4 copies/g, respectively, indicating that, even for the LAB species showing potential survival in the gut, competition in this niche may be harsh and the population may substantially decrease during the digestion process. It may be difficult for silage LAB to survive in the gut of silage-fed dairy cows, because marked decrease in population can take place during the digestion process, even for surviving species.

    DOI: 10.1111/asj.12896

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  • Variability, stability, and resilience of fecal microbiota in dairy cows fed whole crop corn silage 査読

    Minh Thuy Tang, Hongyan Han, Zhu Yu, Takeshi Tsuruta, Naoki Nishino

    Applied Microbiology and Biotechnology   101 ( 16 )   6355 - 6364   2017年8月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The microbiota of whole crop corn silage and feces of silage-fed dairy cows were examined. A total of 18 dairy cow feces were collected from six farms in Japan and China, and high-throughput Illumina sequencing of the V4 hypervariable region of 16S rRNA genes was performed. Lactobacillaceae were dominant in all silages, followed by Acetobacteraceae, Bacillaceae, and Enterobacteriaceae. In feces, the predominant families were Ruminococcaceae, Bacteroidaceae, Clostridiaceae, Lachnospiraceae, Rikenellaceae, and Paraprevotellaceae. Therefore, Lactobacillaceae of corn silage appeared to be eliminated in the gastrointestinal tract. Although fecal microbiota composition was similar in most samples, relative abundances of several families, such as Ruminococcaceae, Christensenellaceae, Turicibacteraceae, and Succinivibrionaceae, varied between farms and countries. In addition to the geographical location, differences in feeding management between total mixed ration feeding and separate feeding appeared to be involved in the variations. Moreover, a cow-to-cow variation for concentrateassociated families was demonstrated at the same farm; two cows showed high abundance of Succinivibrionaceae and Prevotellaceae, whereas another had a high abundance of Porphyromonadaceae. There was a negative correlation between forage-associated Ruminococcaceae and concentrate-associated Succinivibrionaceae and Prevotellaceae in 18 feces samples. Succinivibrionaceae, Prevotellaceae, p-253418B5, and Spirochaetaceae were regarded as highly variable taxa in this study. These findings help to improve our understanding of variation and similarity of the fecal microbiota of dairy cows with regard to individuals, farms, and countries. Microbiota of naturally fermented corn silage had no influence on the fecal microbiota of dairy cows.

    DOI: 10.1007/s00253-017-8348-8

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  • Comparative microbiota assessment of wilted Italian ryegrass, whole crop corn, and wilted alfalfa silage using denaturing gradient gel electrophoresis and next-generation sequencing 査読

    Kuikui Ni, Tang Thuy Minh, Tran Thi Minh Tu, Takeshi Tsuruta, Huili Pang, Naoki Nishino

    Applied Microbiology and Biotechnology   101 ( 4 )   1385 - 1394   2017年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The microbiota of pre-ensiled crop and silage were examined using denaturing gradient gel electrophoresis (DGGE) and next-generation sequencing (NGS). Wilted Italian ryegrass (IR), whole crop corn (WC), and wilted alfalfa (AL) silages stored for 2 months were examined. All silages contained lactic acid as a predominant fermentation product. Across the three crop species, DGGE detected 36 and 28 bands, and NGS identified 253 and 259 genera in the pre-ensiled crops and silages, respectively. The NGS demonstrated that, although lactic acid bacteria (LAB) became prevalent in all silages after 2 months of storage, the major groups were different between crops: Leuconostoc spp. and Pediococcus spp. for IR silage, Lactobacillus spp. for WC silage, and Enterococcus spp. for AL silage. The predominant silage LAB genera were also detected by DGGE, but the presence of diverse non-LAB species in pre-ensiled crops was far better detected by NGS. Likewise, good survival of Agrobacterium spp., Methylobacterium spp., and Sphingomonas spp. in IR and AL silages was demonstrated by NGS. The diversity of the microbiota described by principal coordinate analysis was similar between DGGE and NGS. Our finding that analysis of pre-ensiled crop microbiota did not help predict silage microbiota was true for both DGGE and NGS.

    DOI: 10.1007/s00253-016-7900-2

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  • Dietary casein and soy protein isolate modulate the effects of raffinose and fructooligosaccharides on the composition and fermentation of gut microbiota in rats 査読

    Gaowa Bai, Kuikui Ni, Takeshi Tsuruta, Naoki Nishino

    Journal of Food Science   81 ( 8 )   H2093 - H2098   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Although diet has an important influence on the composition of gut microbiota, the impact of dietary protein sources has only been studied to a minor extent. In this study, we examined the influence of different dietary protein sources regarding the effects of prebiotic oligosaccharides on the composition and metabolic activity of gut microbiota. Thirty female rats were fed casein and soy protein isolate with cellulose, raffinose (RAF), and fructooligosaccharides (FOS). Microbiota composition was examined by real-time qPCR and denaturing gradient gel electrophoresis. Dietary protein source affected cecum microbiota; acetic acid concentration and Lactobacillus spp. populations were greater with soy protein than with casein. Prebiotic oligosaccharides had distinctive effects on gut microbiota; RAF increased the acetic acid concentration and Bifidobacterium spp. populations, and FOS increased the butyric acid concentration regardless of the dietary protein. Likewise, Bifidobacterium sp., Collinsella sp., and Lactobacillus sp. were detected in microbiota of the rats fed RAF, and Bacteroides sp., Roseburia sp., and Blautia sp. were seen in microbiota of the rats fed FOS. Interactions between dietary proteins and prebiotic oligosaccharides were observed with Clostridium perfringens group populations and cecum IgA concentration. RAF and FOS decreased C. perfringens group populations in casein-fed rats, and the combination of soy protein and RAF substantially increased cecum IgA concentration. These results indicate that dietary proteins can differentially modulate the effects of prebiotic oligosaccharides on gut fermentation and microbiota, depending on the type of carbohydrate polymers involved.

    DOI: 10.1111/1750-3841.13391

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  • Lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration 査読

    Li, Y., Wang, F., Nishino, N.

    Asian-Australasian Journal of Animal Sciences   29 ( 4 )   516 - 522   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC  

    We investigated the effects of the predominant lactic acid bacteria (LAB) on the fermentation characteristics and aerobic stability of total mixed ration (TMR) silage containing soybean curd residue (SC-TMR silage). The SC-TMR materials were ensiled in laboratory silos for 14 or 56 days. LAB predominant in SC-TMR silage were identified (Exp. 1). Lactobacillus fermentum (L. fermentum) and Streptococcus bovis (S. bovis) were found in the untreated materials, Leuconostoc pseudomesenteroides (L. pseudomesenteroides) in 14-day silage and Lactobacillus plantarum (L. plantarum) in all silages. Pediococcus acidilactici (P. acidilactici), Lactobacillus paracasei (L. paracasei), and Lactobacillus brevis (L. brevis) formed more than 90% of the isolates in 56-day silage. Italian ryegrass and whole crop maize were inoculated with P. acidilactici and L. brevis isolates and the fermentation and aerobic stability determined (Exp. 2). Inoculation with P. acidilactici and L. brevis alone or combined improved the fermentation products in ryegrass silage and markedly enhanced its aerobic stability. In maize silage, P. acidilactici and L. brevis inoculation caused no changes and suppressed deterioration when combined with increases in acetic acid content. The results indicate that P. acidilactici and L. brevis may produce a synergistic effect to inhibit SC-TMR silage deterioration. Further studies are needed to identify the inhibitory substances, which may be useful for developing potential antifungal agents.

    DOI: 10.5713/ajas.15.0267

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  • Identification and isolation of Lactobacillus fructivorans from wilted alfalfa silage with and without molasses 査読

    B. Wu, N. Nishino

    Journal of Applied Microbiology   120 ( 3 )   543 - 551   2016年3月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    AimTo gain insights into the bacterial species associated with anaerobic storage and aerobic stability of alfalfa silage.
    Methods and ResultsWilted alfalfa silage (498gdry matterkg(-1)) was prepared with and without the addition of molasses. Aerobic spoilage tests were conducted at 5, 10 and 60days after ensiling. The composition of fermentation products and the bacterial communities of silage were determined at 1, 3, 5 and 7days after silo opening. Silage without molasses had small amounts of lactic and acetic acids detectable at silo opening but resisted deterioration due to aerobic spoilage for at least 5days after opening. Resistance to aerobic deterioration in silage increased with the addition of molasses. The predominant bacterial species in molasses-added silage was Lactobacillus fructivorans, which was detected by denaturing gradient gel electrophoresis analysis. Different bacterial growth media were used for Lact.fructivorans isolation from alfalfa silage with added molasses: isolation was successful using liver infusion sake medium, but was unsuccessful when de Man, Rogosa and Sharpe medium was used.
    ConclusionA nonconventional lactic acid bacterium (LAB) species may be involved in the high aerobic stability of alfalfa silage.
    Significance and Impact of the StudyThe findings demonstrate that culture-independent microbiota analysis may be useful in the isolation and identification of nonconventional LAB species involved in fermentation and the aerobic stability of silage.

    DOI: 10.1111/jam.13031

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  • Fermentation and microbial population dynamics during the ensiling of native grass and subsequent exposure to air 査読

    Qing Zhang, Baiyila Wu, Naoki Nishino, Xianguo Wang, Zhu Yu

    Animal Science Journal   87 ( 3 )   389 - 397   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    To study the microbial population and fermentation dynamics of large needlegrass (LN) and Chinese leymus (CL) during ensiling and subsequent exposure to air, silages were sampled and analyzed using culture-based techniques and denaturing gradient gel electrophoresis (DGGE). A total of 112 lactic acid bacteria (LAB) strains were isolated and identified using the 16S rRNA sequencing method. Lactic acid was not detected in the first 20days in LN silage and the pH decreased to 6.13 after 45days of ensiling. The temperature of the LN silage increased after approximately 30h of air exposure and the CL silage showed a slight temperature variation. Enterococcus spp. were mainly present in LN silage. The proportion of Lactobacillus brevis in CL silage increased after exposure to air. LN silage with a higher proportion of Enterococcus spp. and propionic acid concentration did not show higher fermentation quality or aerobic stability than CL silage, which had a higher concentration of acetic acid, butyric acid and increased proportion of L. brevis after exposure to air.

    DOI: 10.1111/asj.12427

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  • Identification of bacteria in total mixed ration silage produced with and without crop silage as an ingredient 査読

    Naoki Nishino, Yu Ogata, Hongyan Han, Yasunari Yamamoto

    Animal Science Journal   86 ( 1 )   45 - 50   2015年1月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    As a forage source for total mixed ration (TMR) silage production, locally produced crop silage is now used in addition to imported hay. This type of TMR ensiling is regarded as a two-step fermentation process; hence, a survey was carried out to determine whether the bacteria in crop silage affect the subsequent TMR ensiling. Fermentation product contents and bacterial community were determined for TMR silage and its ingredient silages collected in August, October and November. August product contained corn, sorghum and Italian ryegrass silages, October product had wheat silage exclusively and November product did not include any crop silages. Acetic acid, lactic acid, 2,3-butanediol and ethanol were predominant fermentation products in corn, sorghum, Italian ryegrass and wheat silages, respectively. Robust lactic acid fermentation was seen in TMR silage, even if acetate-type and alcohol-type silages were mixed as ingredients. The finding that bacterial community of the TMR silage appeared unrelated to those of ingredient silage supported this. Silages of various fermentation types can therefore be formulated without interfering with lactate-type fermentation in TMR silage.

    DOI: 10.1111/asj.12234

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  • Bacterial communities in alfalfa and corn silages produced in large-scale stack and bunker silos in China 査読

    Baiyila Wu, Qing Zhang, Zhongkuan Liu, Zhu Yu, Naoki Nishino

    Grassland Science   60 ( 4 )   247 - 251   2014年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Alfalfa silage (Al) and corn silage (Cs) samples were collected from two large-scale farms in China; one of these farms used stack silos and the other used bunker silos for silage production. Three samples were collected from the upper and lower layers of each silo. Thus, six samples were examined for each of Al-stack, Al-bunker, Cs-stack, and Cs-bunker. Bacterial community was determined by denaturing gradient gel electrophoresis (DGGE). Lactic acid was the main fermentation product in Al-stack, Cs-stack and Cs-bunker, whereas acetic acid and butyric acid were predominant in Al-bunker. Pediococcus pentosaceus and Clostridium sp. were common to four silage types. Lactobacillus acetotolerans and Lactobacilluspontis were detected specifically in Cs, whereas the lactic acid bacteria identified in Al were regarded as those usually found in silage. Differences between stack and bunker silos were apparent in Al with regard to fermentation products and DGGE band patterns. Differences between the upper and lower layers were observed in the fermentation products in Cs regardless of silo type, whereas variations in the DGGE band patterns of Cs-stack were detected.

    DOI: 10.1111/grs.12063

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  • A pilot examination of the fermentation products, aerobic stability and bacterial community of total mixed ration silage produced in Vietnam 査読

    Tu Tran Thi Minh, Van Nguyen Huu, Naoki Nishino

    Grassland Science   60 ( 1 )   63 - 68   2014年3月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    To evaluate the fermentation characteristics and aerobic stability of total mixed ration (TMR) silage produced in the tropics, rice straw (Rs) and corn stover (Cst) were mixed with molasses (M) and other feeds as TMRs, which were then preserved as silage in Hue, Vietnam. The silages were opened after 4months, and the microbial counts, fermentation product levels and aerobic stability were determined. The bacterial community was assessed by denaturing gradient gel electrophoresis (DGGE). All the silages were well preserved, with lactic acid serving as the major preservative. However, the lactic acid content was low (5.99gkg(-1) dry matter [DM]) in the Rs-M silage, while the ethanol content was substantial (37.2gkg(-1)DM) in the Cst-M silage. Heating due to spoilage was observed at 115 and 81h after silo opening in the Cst-M and Cst-TMR silages, respectively, whereas no heating was observed for 7days after the opening of the Rs-M and Rs-TMR silages. Among the 14 bacterial strains identified in the silages by the DGGE analysis, only three were lactic acid bacteria. Lactobacillus brevis and Weissella paramesenteroides were detected in all silages, while W.cibaria was only detected in the Rs-M and Cst-M silages. In the aerobically unstable Cst-TMR silage, bands indicative of Acetobacter pasteurianus, Staphylococcus sp. and Streptomyces sp. were specifically observed. These results indicate that although desirable lactic acid fermentation can be expected in a TMR silage in a tropical environment, aerobic stability is lowered if Cst instead of Rs is used as the ingredient crop. The presence of A.pasteurianus in the air-tight laboratory silo was unusual, but it could account for the low aerobic stability of the Cst-TMR silage.

    DOI: 10.1111/grs.12041

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  • A survey of fermentation products and bacterial communities in corn silage produced in a bunker silo in China 査読

    Chao Wang, Hongyan Han, Xueying Gu, Zhu Yu, Naoki Nishino

    Animal Science Journal   85 ( 1 )   32 - 36   2014年1月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    To evaluate the current practice of corn silage management in China, samples of bunker-made silage were collected from 14 farms within a 500-km radius of Beijing for the analysis of fermentation products and bacterial communities. Mean values for dry matter (DM) content were as low as 250g/kg in both corn stover (St) and whole crop corn (Wc) silages, and pH values averaged 4.48 and 3.73, respectively. Only three of the 14 silages exhibited a lactic-to-acetic acid ratio >1.0, indicating that the presence of acetic acid was predominant in fermentation. Although 1,2-propanediol content was marginal in most cases (<5.0g/kg dry matter (DM)), two Wc silages had 1,2-propanediol levels >25g/kg DM. In contrast, 3 St silages had large amounts (>10g/kg DM) of butyric acid, and two of the three butyrate silages also had high concentrations of 1-propanol. Denaturing gradient gel electrophoresis analysis demonstrated that the bacterial community appeared similar in 10 out of the 14 silage samples. Bands indicating Lactobacillus buchneri, L. acetotolerans and Acetobacter pasteurianus were found in both the St and Wc silages, accounting for the high acetic acid content found across silage samples.

    DOI: 10.1111/asj.12076

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  • Effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content 査読

    Yanbing Li, Naoki Nishino

    Asian-Australasian Journal of Animal Sciences   26 ( 9 )   1304 - 1312   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The effects of storage period and aerobic deterioration on the bacterial community were examined in Italian ryegrass (IR), guinea grass (GG), and whole-crop maize (WM) silages. Direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives
    live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-Butanediol, acetic acid, and lactic acid were the dominant fermentation products in the IR, GG, and WM silages, respectively. The acetic acid content increased as a result of prolonged ensiling, regardless of the type of silage crop, and the changes were distinctively visible from the beginning of GG ensiling. Pantoea agglomerans, Rahnella aquatilis, and Enterobacter sp. were the major bacteria in the IR silage, indicating that alcoholic fermentation may be due to the activity of enterobacteria. Staphylococcus sciuri and Bacillus pumilus were detected when IR silage was spoiled, whereas between aerobically stable and unstable silages, no differences were seen in the bacterial community at silo opening. Lactococcus lactis was a representative bacterium, although acetic acid was the major fermentation product in the GG silage. Lactobacillus plantarum, Lactobacillus brevis, and Morganella morganii were suggested to be associated with the increase in acetic acid due to prolonged storage. Enterobacter cloacae appeared when the GG silage was spoiled. In the WM silage, no distinctive changes due to prolonged ensiling were seen in the bacterial community. Throughout the ensiling, Weissella paramesenteroides, Weissella confusa, and Klebsiella pneumoniae were present in addition to L. plantarum, L. brevis, and L. lactis. Upon deterioration, Acetobacter pasteurianus, Klebsiella variicola, Enterobacter hormaechei, and Bacillus gibsonii were detected. These results demonstrate the diverse bacterial community that evolves during ensiling and aerobic spoilage of IR, GG, and WM silages. © 2013 by Asian-Australasian Journal of Animal Sciences.

    DOI: 10.5713/ajas.2013.13175

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  • Changes in the bacterial community and composition of fermentation products during ensiling of wilted Italian ryegrass and wilted guinea grass silages 査読

    Yanbing Li, Naoki Nishino

    Animal Science Journal   84 ( 8 )   607 - 612   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    To gain further insights into temperate and tropical grass ensiling, fermentation products and bacterial communities were examined at both the initial and late stages of ensiling of wilted Italian ryegrass and wilted guinea grass silages. 2,3-Butanediol and ethanol fermentation were observed in wilted Italian ryegrass silage. Enterobacteria such as Rahnella sp. and Enterobacter sp. may have been involved in fermentation; however, alcohol production was intensified after the silage enterobacterial community overwhelmed the pre-ensiled enterobacterial community. Pediococcus spp. appeared in silage stored for 4 months, when a significant increase in lactic acid content was seen compared with that at 2 months. Prolonged storage enhanced acetic acid fermentation in wilted guinea grass silage. The disappearance of Enterococcus sulfureus and appearance of Lactobacillus plantarum may have been associated with the increased acetic acid content. Although many species of enterobacteria were found in common between the pre-ensiled crop and silages of Italian ryegrass and guinea grass, marked differences were seen in the type of fermentation from the initial stages. These results indicate that the bacterial community of pre-ensiled crops may be immediately replaced by one that is adapted to ensiling environments, although metabolic changes may continue over the course of ensiling.

    DOI: 10.1111/asj.12046

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  • Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri on fermentation, aerobic stability and microbial communities in whole crop corn silage 査読

    Yanbing Li, Naoki Nishino

    Grassland Science   57 ( 4 )   184 - 191   2011年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Whole crop corn silage may be prone to spoilage after exposure to air. To investigate control of fermentation and aerobic spoilage by using silage additives, Lactobacillus rhamnosus and Lactobacillus buchneri were added to whole crop corn silage, and shifts in microbial communities were examined. Bacterial and fungal communities were identified by denaturing gradient gel electrophoresis analysis at the time of opening the silo and after conducting aerobic deterioration tests. Inoculation of L. rhamnosus did not affect fermentation, whereas that of L. buchneri decreased the lactic acid content and increased the acetic acid content. Aerobic stability was enhanced in silage stored for a long period (120 days), with increases in the acetic acid content even without L. buchneri inoculation. A high aerobic stability was achieved with a shorter ensiling period (56 days) in L. buchneri-inoculated silage. Lactic acid bacteria (LAB) inoculation did not alter the indigenous bacterial community, and the inoculated species were detected only as additions. Lactobacillus brevis, Pediococcus parvulus, Weissella confusa and Klebsiella pneumoniae were found in both the pre-ensiled crop and the silage, whereas Weissella paramesenteroides, Lactobacillus plantarum and Lactobacillus lactis were seen exclusively after ensiling. Inoculation also had little effect on the fungal community during the fermentation process. Candida magnolia, Cryptococcus flavus and Candida intermedia were detected, both in the pre-ensiled crop and the silage, whereas Candida glabrata and Candida quercitrusa were identified exclusively in the silage. Marked changes were seen in the fungal community after aerobic spoilage; Saccharomyces exiguus, Saccharomyces martiniae, Pichia fermentans, Pichia deserticola and Pichia kudriavzevii appeared in the spoiled silage. These results indicated that LAB inoculation produces few changes in bacterial and fungal communities in corn silage and that substantial changes occur in the fungal community when the silage is spoiled.

    DOI: 10.1111/j.1744-697X.2011.00226.x

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  • Monitoring the bacterial community of maize silage stored in a bunker silo inoculated with Enterococcus faecium, Lactobacillus plantarum and Lactobacillus buchneri 査読

    Y. Li, N. Nishino

    Journal of Applied Microbiology   110 ( 6 )   1561 - 1570   2011年6月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Aims:
    To monitor variations in the bacterial community and fermentation products of maize silage within and between bunker silos.
    Methods and Results:
    Silage samples were collected in 2008 and 2009 from three dairy farms, wherein the farmers arranged for a contractor to produce maize silage using bunker silos. Silage was prepared using a lactic acid bacteria (LAB) inoculant consisting of Enterococcus faecium, Lactobacillus plantarum and Lactobacillus buchneri. Eight samples were collected from each bunker silo; 4 'outer' and 4 'inner' samples were collected from near the top and the bottom of the silo. The dry matter, lactic acid, acetic acid, ethanol, 1-propanol and 1,2-propanediol contents differed between bunker silos in both sampling years. Higher acetic acid, 1-propanol and 1,2-propanediol contents were found in the bottom than the top layers in the 2008 samples, and higher lactic acid content was found in the top than the bottom layers in the 2009 samples. The bacterial community varied more between bunker silos than within a bunker silo in the 2008 samples, whereas differences between the top and the bottom layers were seen across bunker silos in the 2009 samples. The inoculated LAB were uniformly distributed, while several nonconventional silage bacteria were also detected. Lactobacillus acetotolerans, Lactobacillus panis and Acetobacter pasteurianus were detected in both years. Stenotrophomonas maltophilia was detected in the 2008 samples, and Lactobacillus reuteri, Acinetobacter sp. and Rahnella sp. were detected in the 2009 samples.
    Conclusions:
    Although differences were seen within and between bunker silos, the bacterial community may indicate a different relationship between bunker silos and sampling locations within a bunker silo from that indicated by the fermentation products.
    Significance and Impact of the Study:
    Analysis of bacterial community can help understand how diverse non-LAB and LAB species are involved in the ensiling process of bunker-made maize silage.

    DOI: 10.1111/j.1365-2672.2011.05010.x

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  • Chemical Changes during Ensilage and In sacco Degradation of Two Tropical Grasses: Rhodesgrass and Guineagrass Treated with Cell Wall-degrading Enzymes 査読

    Yu Zhu, Naoki Nishino, Guo Xusheng

    Asian-Australasian Journal of Animal Sciences   24 ( 2 )   214 - 221   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC  

    Effects of the cell wall-degrading enzymes derived from Acremonium cellulolyticus and Trichoderma viride on the silage fermentation and in sacco degradation of tropical grasses i.e. rhodesgrass (Chloris gayana Kunth. cv. Callide) and guineagrass (Panicum maximum Jacq. cv. Natsukaze) were investigated in laboratory-scale experiments. These two grasses were either treated with or without the enzymes before ensiling. Untreated rhodesgrass produced acetate fermentation silage (lactate, 13.0 g kg(-1) DM; acetate, 38.7 g kg(-1) DM) with high final pH value and NH(3)-N content (5.84 and 215 g kg(-1) DM). Addition of enzymes significantly increased (p<0.01) the lactate production (lactate, 45.6; acetate, 34.0 g kg(-1) DM) and decreased (p<0.01) the pH and NH(3)-N (4.80 and 154 g kg(-1) DM) in the ensiled forages when compared with the control silages. Untreated guineagrass was successfully preserved with a high lactate proportion (lactate, 45.5; acetate, 24.1 g kg(-1) DM), and the addition of enzymes further enhanced the desirable fermentation (lactate, 57.5 g kg(-1) DM: acetate, 19.4 g kg(-1) DM). The content of NDF was lowered (p<0.05) by enzymes in both silages, but the extent appeared greater in the enzyme-treated rhodesgrass (rhodesgrass, 48 g kg(-1) DM; guineagrass, 21 g kg(-1) DM). Changes in the kinetics of in sacco degradation showed that enzyme treatment increased (p<0.01) the rapidly degradable DM (rhodesgrass, 299 vs. 362 g kg(-1) DM; guineagrass, 324 vs. 343 g kg(-1) DM) but did not influence the potential degradation, lag time and degradation rate of DM and NDF in the two silages.

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  • Presence of sourdough lactic acid bacteria in commercial total mixed ration silage as revealed by denaturing gradient gel electrophoresis analysis(共著)

    Wang, C, Nishino, N

    Letters in Applied Microbiology   51 ( 4 )   436 - 442   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1472-765X.2010.02915.x

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  • Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and rhodes grass silages(共著)

    Parvin, S, Wang, C, Li, Y, Nishino, N

    Animal Feed Science and Technology   160 ( 3-4 )   160 - 166   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.anifeedsci.2010.07.010

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  • Succession of lactic acid bacteria in wilted rhodesgrass silage assessed by plate culture and denaturing gradient gel electrophoresis(共著) 査読

    Parvin, S, Nishino, N

    Grassland Science   56 ( 1 )   51 - 55   2010年3月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1744-697X.2009.00173.x

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  • Bacterial community associated with ensilage process of wilted guinea grass(共著) 査読

    Parvin, S, Nishino, N

    Journal of Applied Microbiology   107 ( 6 )   2029 - 2036   2009年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1365-2672.2009.04391.x

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  • Association of Lactobacillus buchneri with aerobic stability of total mixed ration containing wet brewers grains preserved as a silage(共著) 査読

    Wang, F.J, Nishino, N

    Animal Feed Science and Technology   149 ( 3-4 )   265 - 274   2009年3月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.anifeedsci.2008.06.012

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  • Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration 査読

    F. Wang, N. Nishino

    Journal of Dairy Science   91 ( 6 )   2380 - 2387   2008年6月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER DAIRY SCIENCE ASSOC  

    Wet brewers grains and soybean curd residue were stored in laboratory-scale silos without (BG and SC silages, respectively) or with other ingredients as total mixed rations (BGT and SCT silages, respectively). Silages were opened after 14 and 56 d, and microbial counts, fermentation products, and aerobic stability were determined. Denaturing gradient gel electrophoresis was carried out to examine bacterial communities, and several bacteria that appeared to be involved in fermentation were identified. Lactic acid content was greater in SCT than in BGT silage, but lower in SC than in BG silage. Ethanol content was greater in BG than in SC regardless of silage type. Aerobic deterioration occurred promptly in ensiling materials (nonensiled by-products and total mixed ration mixtures) and in silages stored alone; however, SCT and BGT silages resisted deterioration and no heating was found for more than 5.5 d regardless of storage period. Silages were stable even with high yeast populations at silo opening, whereas prolonged ensiling decreased yeast counts in the 2 total mixed ration silages. The denaturing gradient gel electrophoresis profiles appeared similar between SCT and BGT silages but not between SC and BG silages. Weissella spp. and Lactobacillus brevis were common in aerobically stable SCT and BGT silages, and Lactobacillus buchneri was detected only in BGT silage. Both L. brevis and L. buchneri were found in silage but not in ensiling materials. Several other lactic acid bacteria were also identified in SCT and BGT silages, but did not appear to be related to fermentation and aerobic stability.

    DOI: 10.3168/jds.2007-0821

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  • Variations in bacterial communities in laboratory-scale and big bale silos assessed by fermentation products, colony counts and denaturing gradient gel electrophoresis profiles 査読

    Nishino N, Tanabe Y

    Letters in Applied Microbiology   46 ( 3 )   283 - 288   2008年3月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Aims: To assess the variation in bacterial communities in laboratory-scale and big bale silos.
    Methods and Results: Wilted Italian ryegrass (628 g dry matter kg(-1)) was ensiled in vacuum-packed plastic pouches and big bales. Silos were opened after 3 months, and the fermentation products, colony counts and denaturing gradient gel electrophoresis (DGGE) profiles were determined. Eight samples were collected separately from a big bale, while one representative sample was taken from a plastic pouch. Significant variation was found between big bales in dry matter, ethanol, lactic acid, acetic acid and ammonia-N contents. No differences were shown between plastic pouches and big bales, except that more ethanol was produced in the former air-tight silos. Plastic pouches could resemble a specific silo and outer sampling sites of big bales based on fermentation products and DGGE profiles respectively.
    Conclusions: Considerable variation in fermentation products may exist between big bale silos. Plastic pouches can serve as a model of big bale silos, although they do not provide information on the heterogeneity within and between bales.
    Significance and Impact of the Study: Assessment of bacterial communities associated with ensiling can differ according to the criteria of fermentation products, colony counts and DGGE profiles.

    DOI: 10.1111/j.1472-765X.2007.02306.x

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  • Resistance to aerobic deterioration of total mixed ration silage: effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability 査読

    Fujin Wang, Naoki Nishino

    Journal of the Science of Food and Agriculture   88 ( 1 )   133 - 140   2008年1月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    BACKGROUND: High aerobic stability can be expected when wet brewers' grains are stored as a total mixed ration (TMR) silage. To understand the factors affecting the stability, the effects of ration formulation, air infiltration and storage period were studied.
    RESULTS: A TMR containing wet brewers' grains, hay, maize, wheat bran, beet pulp and molasses was ensiled in laboratory silos for 14 and 56 days. The effects of hay species (lucerne or sudangrass) and air infiltration (Exp. 1) and of excluding one, two or three items from the six ingredients (Exp. 2) were examined. Ethanol was the main fermentation product in all TMR silages in this study. Aerobic deterioration occurred in 14 day silages prepared with sudangrass hay along with air infiltration (Exp. 1), and with the simplest recipe where three items (hay, maize and wheat bran) were excluded (Exp. 2). No deterioration occurred in 56 day silages regardless of ration formulation and air infiltration. Yeasts receded in 56 day silages, except with the simplest recipe, to the 102 cfu g(-1) level and remained undetectable in the presence of air (Exp. 2).
    CONCLUSION: TMR silage can resist aerobic deterioration provided that a sufficient ensiling period has elapsed. Silages stored for only a few weeks may be susceptible to deterioration when air is infiltrated or where fewer ingredients are used in the TMR mixture. (C) 2007 Society of Chemical Industry.

    DOI: 10.1002/jsfa.3057

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  • Effect of aerobic exposure after silo opening on feed intake and digestibility of total mixed ration silage containing wet brewers’ grains or soybean curd residue. 査読

    Wang, F, Nishino, N

    Grassland Science   54 ( 3 )   164 - 166   2008年

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    担当区分:責任著者  

    DOI: 10.1111/j.1744-697X.2008.00118.x

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  • Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria 査読

    Naoki Nishino, Hirotaka Hattori

    Journal of the Science of Food and Agriculture   87 ( 13 )   2420 - 2426   2007年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS LTD  

    Wet brewers grains were stored as a total mixed ration (TMR) in laboratory silos with lucerne hay, cracked maize, sugar beet pulp, soya bean meal and molasses at 5:1:1:1:1:1 on fresh weight basis. The TMR mixture was inoculated with or without Lactobacillus casei or Lactobacillus buchneri to obtain silages with differing fermentation and stability after exposure to air. In the first experiment, ensiling was stopped at 10, 20 and 60 days, and the stability was tested for the following 7 days. Ethanol and lactic acid were the main products in untreated TMR silage, while addition of L. casei and L. buchneri increased lactic and acetic acid, respectively. No silages deteriorated in the presence of air over 7 days, regardless of inoculation, ensiling period and the level of yeasts determined at unloading. In the second experiment, silos were opened at 14 days and then subjected to aerobic stability test for 14 days. Resistance to deterioration was sustained in the untreated control, even with a high population (> 10(4) cfu g(-1)) of yeasts throughout the 14-day test. Spoilage was found in L. casei-treated silage at about 5 days, while increase of yeasts preceded the distinct heating (degradation). In L. buchneri-treated silage, no yeasts were detected at unloading or after exposure to air. These results suggest that substantial stability can be expected in TMR silage with or without inoculation of lactic acid bacteria. This property is not associated with the counts of yeasts at loading and the characteristics of silage such as alcoholic and lactic acid fermentation. (C) 2007 Society of Chemical Industry.

    DOI: 10.1002/jsfa.2911

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  • Biogenic amine production in grass, maize and total mixed ration silages inoculated with Lactobacillus casei or Lactobacillus buchneri 査読

    N. Nishino, H. Hattori, H. Wada, E. Touno

    Journal of Applied Microbiology   103 ( 2 )   325 - 332   2007年8月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Aims: To investigate the effects of inoculating Lactobacillus casei or Lacobacillus buchneri on the production of biogenic amines (BA) in silage.
    Methods and Results: Wilted festulolium (Lolium perenne x Festuca pratensis), whole crop maize or a total mixed ration, consisting of wet brewer grains, lucerne hay, cracked maize, sugarbeet pulp, soyabean meal and molasses, was ensiled with or without the inoculation of either L. casei (> 10(6) CFU g(-1)) or L. buchneri (> 10(6) CFU g(-1)). Silages were opened after 60 days of storage, and the concentrations of histamine, tyramine, putrescine and cadaverine were determined. The inoculation of L. casei decreased all the BA regardless of the silage type. The effects of L. buchneri varied between the three silages; the tyramine and putrescine were increased in maize but were lowered in festulolium. Histamine was reduced in fiestulolium and the by-products, whereas no change was found in the maize silage. None of the inoculant strains produced the four BA in a synthetic medium, accounting for the actual ensiling except for tyramine and putrescine in maize.
    Conclusions: Wide variation would be found in the production of BA owing to the ensiling materials. The inoculation of L. casei can lower the BA concentration, while the effects of L. buchneri may vary considerably. The screening of BA-producing activity may help to reduce the risk of BA contamination in inoculated silage.
    Significance and Impact of the Study: Strains of decarboxylase-negative L. buchneri can enhance the aerobic stability of silage without a concern regarding the large production of putrefactive BA.

    DOI: 10.1111/j.1365-2672.2006.03244.x

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  • Changes during ensilage in fermentation products, tea catechins, antioxidative activity and in vitro gas production of green tea waste stored with or without dried beet pulp 査読

    Naoki Nishino, Tetsuya Kawai, Makoto Kondo

    Journal of the Science of Food and Agriculture   87 ( 9 )   1639 - 1644   2007年7月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS LTD  

    Wet green tea waste (GTW) was ensiled in laboratory silos with or without dried beet pulp at a ratio of 2:1 on a fresh weight basis. The silos were opened at 15, 30 and 60 days after anaerobic storage; fermentation products, tea catechins, antioxidative activity and in vitro gas production were determined. Acetic acid prevailed over the fermentation and the pH declined to below 4.2 when wet GTW was ensiled alone. Addition of dried beet pulp greatly enhanced lactic acid production and the pH further decreased to below 3.8 with no butyric acid being detected. Approximately 90% and 80% of (-)-epicatechin gallate and (-)-epigallocatechin gallate, respectively, were degraded in silage prepared from wet GTW alone, while the degradation was ameliorated in mixed silage to about 55% and 70%, respectively. Antioxidative activity was not markedly altered by ensiling and 70-80% of the initial activity was detected after 60 days. Changes were small in (-) -epigallocatechin, caffeine and total phenols with or without mixing with dried beet pulp. In vitro gas production was similar between materials and their silages, indicating that reduction of tea catechins would not elicit an improvement in digestibility. It is concluded that wet GTW can be ensiled successfully without bacterial inoculants when mixed with other materials containing certain sugars. Ensiling may degrade part of the components of tea catechins, while not affecting total phenols, antioxidative activity and digestibility of wet GTW. (c) 2007 Society of Chemical Industry.

    DOI: 10.1002/jsfa.2842

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  • Alcoholic fermentation and its prevention by Lactobacillus buchneri in whole crop rice silage 査読

    N. Nishino, H. Hattori, Y. Kishida

    Letters in Applied Microbiology   44 ( 5 )   538 - 543   2007年5月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    To evaluate the fermentation characteristics and the effects of Lactobacillus buchneri inoculation in ensiling whole crop rice.
    Laboratory-scale silages were prepared from whole crop rice harvested at yellow-ripe stage. The crop was ensiled for 2 months with and without inoculation of L. buchneri at 10(4), 10(5) and 10(6) CFU g(-1). The effect of prolonged ensiling was also studied by using the same crop; the silos were opened at 1, 3, 6 and 12 months, while the inoculation was made at 10(5) CFU g(-1). Enhanced alcoholic fermentation was found in untreated silage; the sum of ethanol and 2,3-butanediol were seven times higher at 2 months than those of lactic and volatile fatty acids, while the differences were diminished at 12 months owing to the reduction of ethanol in the late ensiling period. Inoculation of L. buchneri inhibited the alcohols; however, ethanol yet prevailed over the fermentation until 6 months, after which acetic acid became the main product in the inoculated silage. Regardless of inoculation and ensiling period, yeasts were not found in whole crop rice silage.
    Substantial amounts of ethanol and 2,3-butanediol would be produced in silage prepared from whole crop rice. The alcoholic fermentation can be suppressed when inoculated with L. buchneri.
    Inoculation of L. buchneri could be an option to prevent ethanol fermentation in silage.

    DOI: 10.1111/j.1472-765X.2006.02105.x

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  • Ethanol and 2,3-butanediol production in whole-crop rice silage. 査読

    Nishino, N, Shinde, S

    Grassland Science   53 ( 3 )   196 - 198   2007年

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    担当区分:筆頭著者, 責任著者  

    DOI: 10.1111/j.1744-697X.2007.00089.x

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  • Potential nutritive value of selected browse species from Kenya using in vitro gas production technique and polyethylene glycol. 査読

    Osuga, I. M, Abdulrazak, S. A, Nishino, N, Ichinohe, T, Fujihara, T

    Livestock Research for Rural Development   2006年

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  • Ensiling characteristics and aerobic stability of direct-cut and wilted grass silages inoculated with Lactobacillus casei or Lactobacillus buchneri 査読

    N Nishino, E Touno

    Journal of the Science of Food and Agriculture   85 ( 11 )   1882 - 1888   2005年8月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Two experiments were conducted to study the effects of wilting and inoculating Lactobacillus casei or Lactobacillus buchneri on the fermentation and aerobic stability of grass silages. Chopped Italian ryegrass (IR) and Festulolium (FE) were ensiled with or without wilting and added L casei (> 10(6) Cfu g(-1) fresh matter (FM)) or L buchneri (> 10(6) cfu g(-1) FM). Silos were opened after 120 days and microbial counts, fermentation products and aerobic stability were determined. Addition of L casei increased lactic acid and decreased acetic acid and dry matter loss regardless of wilting and forage species. Inoculation of L buchneri decreased lactic acid and increased acetic acid and 1,2-propanediol, while the effects appeared greater in direct-cut than in wilted grass silages. Although 1,2-propanediol accumulated in FE silage, the diol was degraded further to propionic acid and 1-propanol in IR silage. The activity of 1,2-propanediol degradation was lowered when IR was wilted prior to ensiling; 1,2-propanediol remained and the production of propionic acid and 1-propanol was less than one-third of that in direct-cut silage. Regardless of forage species, addition of L buchneri increased dry matter loss compared with the untreated control, whereas the loss was not significant in. wilted silages. Ammonia production was increased by L buchneri in direct-cut but not in wilted silages. No heating was observed with or without inoculation in direct-cut IR silage after exposure to air. Other silages were deteriorated when L buchneri was not inoculated, while the spoilage was accelerated when L casei was added to FE. (c) 2005 Society of Chemical Industry.

    DOI: 10.1002/jsfa.2189

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  • Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri 査読

    N Nishino, H Wada, M Yoshida, H Shiota

    Journal of Dairy Science   87 ( 8 )   2563 - 2570   2004年8月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER DAIRY SCIENCE ASSOC  

    Whole crop corn (DM 29.2%) and a total mixed ration (TMR, DM 56.8%) containing wet brewers grains, alfalfa hay, dried beet pulp, cracked corn, soybean meal, and molasses at a ratio of 5: 1: 1: 1: 1: 1 on fresh weight basis, were ensiled with and without Lactobacillus casei or Lactobacillus buchneri in laboratory silos. The effects of inoculation on microbial counts, fermentation products, and aerobic stability were determined after 10 and 60 d. Untreated corn silage was well preserved with high lactic acid content, whereas large numbers of remaining yeasts resulted in low stability on exposure to air. Inoculation with L. casei suppressed heterolactic fermentation, but no improvements were found in aerobic stability. The addition of L. buchneri markedly enhanced the aerobic stability, while not affecting the DM loss andNH(3)-N production. Large amounts of ethanol were found when the TMR was ensiled, and the content of ethanol overwhelmed that of lactic acid in untreated silage. This fermentation was related to high yeast populations and accounted for a large loss of DM found in the initial 10 d. The ethanol production decreased when inoculated with L. casei and L. buchneri, but the effects diminished at 60 d of ensiling. Inoculation with L. buchneri lowered the yeasts in TMR silage from the beginning of storage; however, the populations decreased to undetectable levels when stored for 60 d, regardless of inoculation. No heating was observed in TMR silage during aerobic deterioration test for 7 d. This stability was achieved even when a high population of yeasts remained and was not affected by either inoculation or ensiling period. The results indicate that inoculation with L. buchneri can inhibit yeast growth and improve aerobic stability of corn and TMR silage; however, high stability of TMR silage can be obtained even when no treatments were made and high population (> 10(5) cfu/g) of yeasts were detected.

    DOI: 10.3168/jds.S0022-0302(04)73381-0

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  • Enhanced lactic acid fermentation of silage by the addition of green tea waste 査読

    M Kondo, N Naoki, K Kazumi, H Yokota

    Journal of the Science of Food and Agriculture   84 ( 7 )   728 - 734   2004年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS LTD  

    The effects of green tea waste (GTW) addition on the ensiling of forage were investigated. Wet and dried GTW added at 10, 50, 100 and 200g kg(-1) of fresh matter (FM) and at 2, 10 and 20g kg(-1) FM, respectively, decreased pH and increased lactic acid concentration of the silages, whereas the butyric acid concentration and ammonia nitrogen content, as a proportion of a total nitrogen, were lowered, compared with silage without additives (control). To investigate the effect of GTW-associated LAB on silage fermentation, wet GTW was sterilized by autoclaving or gamma irradiation and added at 50 g kg(-1) FM. The silages made with sterilized GTW showed higher lactic acid concentrations, and lower pH and butyric acid concentrations than controls. The counts of lactic acid bacteria (LAB) were higher in silages made with sterilized GTW than control until 10 days after ensiling. The enhanced lactic acid fermentation was not found when green tea polyphenols (GTP) were added. These data suggested that GTW could enhance LAB growth and lactic acid production of silage, particularly when added at 50 g kg(-1) FM in a wet form or at the equivalent in a dry form. Although neither GTW-associated LAB nor GTP accounted for the enhancement of lactic acid fermentation, GTW would possibly supply some nutrients which are heat-stable and effective for LAB growth during silage fermentation.. (C) 2004 Society of Chemical Industry.

    DOI: 10.1002/jsfa.1726

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  • Evaluation of fermentation and aerobic stability of wet brewers' grains ensiled alone or in combination with various feeds as a total mixed ration 査読

    N Nishino, H Harada, E Sakaguchi

    Journal of the Science of Food and Agriculture   83 ( 6 )   557 - 563   2003年5月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS LTD  

    Wet brewer's grains (BG; 200 g kg(-1) dry matter(DM)) were ensiled alone or in combination with various dry feeds as a total mixed ration (TMR; 540 g kg(-1) DM) in laboratory silos. Ensilage was stopped at 40 days (experiment 1) or at 5, 20, 40 and 60 days (experiment 2) after storage. The composition of soluble sugars and the profiles of fermentation products were determined. The 60 day silages were subjected to aerobic deterioration and changes in silage temperature were recorded for 7 days. A rapid pH fall coupled with accelerated lactic acid production was observed in BG silage; soluble sugars, mainly composed of maltose and raffinose, completely disappeared within 5 days of ensiling. Prolonged storage, however, decreased lactic acid concentration and increased acetic acid greatly. Appreciable amounts of propionic acid and I-propanol were also produced in BG silage in the late stages of fermentation. When TMR silage was made, the decline in pH and the increase in lactic acid were delayed due to the low moisture content. Acetic acid production was enhanced from the beginning of storage, and mannitol accumulated in TMR silage. Yeast numbers were lower in TMR than in BG silage, but the changes were limited in the late stages of fermentation. When exposed to air, the temperature of BG silage increased after 3 days, whereas that of TMR silage appeared stable during the 7 days test. The results indicate that BG is a suitable by-product for ensiling and, when ensiled with various feeds as a TMR, improved stability against aerobic deterioration can be expected. (C) 2003 Society of Chemical Industry.

    DOI: 10.1002/jsfa.1395

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  • ビール粕と発泡酒粕から調製したTMR型混合サイレージにおける発酵生成物と単少糖類の変化. 査読

    西野直樹, 原田宏明, 坂口 英

    日本草地学会誌   2003年

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  • Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri 査読

    N Nishino, M Yoshida, H Shiota, E Sakaguchi

    Journal of Applied Microbiology   94 ( 5 )   800 - 807   2003年

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING LTD  

    Aims: To assess the effects of inoculation of Lactobacillus buchneri on the ensiling properties and aerobic stability of maize silage.
    Methods and Results: Chopped whole crop maize was ensiled in 0.5 litre airtight polyethylene bottles (0.4 kg per bottle) and in double-layered, thin polyethylene bags (15 kg per bag), with or without inoculation of Lact. buchneri . The silos were stored for two to four months and the chemical composition, microbial numbers and aerobic stability were determined. Inoculation lowered lactic acid and yeasts, and increased acetic acid and pH value, resulting in improved aerobic stability of the silages. Inoculated silages produced 1,2-propanediol, the content of which increased as ensiling was prolonged, and nearly 50 g kg(-1) dry matter had accumulated after four months of storage. The effects of inoculation, however, were much less pronounced in silages prepared in bags. Mannitol was found in all silages; the production was lowered by Lact. buchneri treatment and appeared to be unrelated to the accumulation of 1,2-propanediol.
    Conclusions: Inoculation of Lact. buchneri occasionally causes accumulation of 1,2-propanediol in silages without further degradation into propionic acid and 1-propanol.
    Significance and Impact of the Study: Substantial amounts of 1,2-propanediol could be consumed by ruminants when fed on silages inoculated with Lact. buchneri . In addition to increasing acetic acid, attention needs to be paid to 1,2-propanediol because the two fermentation products might affect the intake and utilization of silage-based diets.

    DOI: 10.1046/j.1365-2672.2003.01810.x

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  • Content of phenolics and tannins in leaves and pods of some Acacia and Dichrostachys species and effects on in vitro digestibility 査読

    CDK Rubanza, M Shem, R Otsyina, N Nishino, T Ichinohe, T Fujihara

    Journal of Animal and Feed Sciences   12 ( 3 )   645 - 663   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KIELANOWSKI INST ANIMAL PHYSIOLOGY NUTRITION  

    Four browse legume foliages (leaves and pod fruits) from three species of Acacia (A. polyacantha, A. tortilis, A. nilotica) and Dichrostachys sp. native to Tanzania were evaluated for nutritive potential to establish levels and effect of normal phenolics and tannins antinutritive factors (ANFs) on in vitro feed digestibility (IVD). Total extractable phenolics (TP), tannins (TT) were estimated by Folin-Ciocalteu assay, and condensed tannins (CT) by butanol/HCl assay. In leaves, total proanthocyanidins were assayed into constituent anthocyanidins' flavonoids; by high performance liquid chromatography (HPLC). Organic matter degradability (OMD) was estimated in vitro by gas production technique. Adverse effects of tannin's ANF on IVD were assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP) varied (P<0.05) between fodder species and foliages. Leaves had (P<0.05) higher CP (141-194 g/kg DM) in Dichrostachys sp. and A polyacantha, respectively, compared to pods (133-142 g/kg DM) in A. tortilis and Dichrostachys sp., respectively. Neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) varied (P<0.05) between fodder species and foliages. A. nilotica had lowest (P<0.05) NDF, ADF and ADL (182, 68 and 44 g/kg DM respectively). A. polyacantha and Dichrostachys sp. had highest (P<0.05) NDF and ADF (416 and 146 g/kg DM, respectively). Pods had higher (P<0.05) fibre values than leaves. Leaves had higher (P<0.05) TP, TT, and CT (162 138, and 55 mg/g DM), than pods (112, 105 and 35 mg/g DM), respectively. Proanthocyanidin flavonoids (mg/g DM) varied (P<0.05) from 0.062 (A. nilotica) to 5.288 (A. tortilis), 0.188 (A. tortilis) to 4.179 (A. polyacantha), and 0.009 (A. nilotica) to 4.392 (Dichrostachys sp.) for delphinidins, cyanidins and pelargonidin, respectively. The browse foliages had relatively high tannin content greater than 5% DM, a beneficial level in animal feeding and nutrition, and thus could impair feed digestibility. OMD varied significantly (P<0.05) both between fodder species and foliage components. Pods had higher (P<0.05) gas production (GP) and OMD than leaves. Addition of PEG improved (P<0.05) gas production, IVD and metabolizable energy (ME) in leaves and pods by binding tannins. Leaves had higher response on gas production and IVD due to PEG treatment compared to pods except A. nilotica. Improved gas production and digestibility due to PEG treatment indicate nutritive potential in browse fodder previously depressed by tannin ANFs.
    Therefore, phenolics and tannins ANFs could limit utilization of browse fodder nutritive potential as supplements to ruminants consuming low quality roughages. Utilization of browse legume fodder could be optimized through reduction of tannin antinutritive activity. Under farmers' conditions, optimal utilization of browse could be achieved through feeding a mixture of tanniferous browse with other feeds especially high in nitrogen to dilute tannin antinutritive activity.

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  • Response of intestinal proteinase activities to the feeding of isolated winged bean (Psophocarpus tetragonolobus) and soya bean (Glycine max) trypsin inhibitors in rats 査読

    N Nishino, M Taniguchi, E Sakaguchi

    Journal of the Science of Food and Agriculture   81 ( 11 )   1055 - 1059   2001年9月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS LTD  

    The antinutritional activities of trypsin inhibitors (TIs) were compared between winged beans (Psophocarpus tetragonolobus) and soya beans (Glycine max). The inhibitors of the two beans were isolated by trypsin-bound Sepharose 4B, and 50 mg of lyophilised powders were intubated intragastrically into 24 h fasted rats. The activities of trypsin and chymotrypsin were compared after 30, 60 and 180 min in the washings of the upper, middle and lower parts of the small intestine. The elution profiles of TI and non-TI compounds in the affinity chromatography were similar in the two beans, and the antitryptic activities were concentrated 5.5 and 6.2 times (based on specific activity) for winged beans and soya beans respectively. Regardless of the TI fed to rats, trypsin activity in the upper intestine was suppressed to almost undetectable levels at 30 and 60 min after intubation. The activities in the middle and lower intestines were also substantially lowered when rats were fed winged bean TI, and significant differences were detected at 30 and 60 min after intubation when compared with rats fed soya bean TI. However, at ISO min after feeding, no differences were found in the trypsin activity in any gut segments. Similar inhibitory properties of isolated TIs were observed in chymotrypsin activities in the small intestine. The results suggest that winged bean TI may have greater inhibitory activity on the intestinal proteinase compared with soya bean TI. (C) 2001 Society of Chemical Industry.

    DOI: 10.1002/jsfa.891

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  • Disappearance of Maillard reaction products during ensilage and rumen fermentation in vitro 査読

    N Nishino, S Uchida

    Journal of the Science of Food and Agriculture   81 ( 2 )   275 - 280   2001年1月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS LTD  

    The disappearance of Maillard reaction products (MRPs) during ensilage and rumen fermentation was studied. MRPs were prepared by heating mixtures of D-glucose and glycine (GG) or D-xylose and glycine (XG); lyophilised powder (MW > 1000) was subjected to anaerobic incubation for 24h with lucerne juice or buffered rumen fluid. Changes in MRPs were assessed by comparing gel filtration profiles before and after incubation, and the proportion of disappeared MRPs was calculated based on the area under the curve. A portion of MRPs, particularly high-MW fractions, disappeared when incubated with lucerne juice, and the extent was greater (P < 0.01) in XG than in GG. The disappearance of GG was increased (P < 0.01) when ensilage was fortified with added glucose, while that of XG was unaffected with or without the fermentable substrate. Rumen fermentation had little impact on GG and XG, whereas a portion of MRPs disappeared when microbial activity was enhanced by adding lucerne hay to the media. Volatile fatty acid production was unaffected by MRPs in either ensilage or rumen fermentation. These results suggest that the effect on MRPs of anaerobic fermentation may be different between ensilage and rumen digestion. Significant amounts of MRPs would enter the intestine when ruminants are fed hay-based diets rather than silage-based diets. (C) 2000 Society of Chemical Industry.

    DOI: 10.1002/1097-0010(20010115)81:2<275::AID-JSFA814>3.0.CO;2-L

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  • 高水分粕類のサイレージ化と利用 3.ビール粕と発泡酒粕から調製したサイレージの発酵特性. 査読

    西野直樹, 原田宏明, 坂口 英

    日本草地学会誌   2001年

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  • Fermentation of rhodesgrass and guineagrass rilags with on without wilting and added cell wall degrading engymes 査読

    Yu, Z, Nishino, N, Uchida, S

    Grassland Science   46   22 - 27   2000年

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  • Ensiling characteristics and ruminal degradation of Italian ryegrass and lucerne silages treated with cell wall degrading enzymes(共著) 査読

    Yu, Z, Nishino, N, Kishida, Y, Uchida, S

    Journal of the Science of Food and Agriculture   79 ( 14 )   1987 - 1992   1999年11月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/(SICI)1097-0010(199911)79:14<1987::AID-JSFA466>3.0.CO;2-J

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  • Laboratory evaluation of previously fermented juice as a fermentation stimulant for lucerne silage(共著) 査読

    Nishino, N, Uchida, S

    Journal of the Science of Food and Agriculture   79 ( 10 )   1285 - 1288   1999年7月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/(SICI)1097-0010(19990715)79:10<1285::AID-JSFA362>3.0.CO;2-M

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  • A note on the chemical changes associated with nonenzymatic browning of forages 査読

    Nishino, N, Touno, E, Uchida, S

    Grassland Science   44   86 - 89   1998年1月

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  • Effects of cell wall degrading enzymes and lactic acid bacteria on the fermentation of rhodes grass silage stored at various ambient temperature(共著) 査読

    Nishino, N, Uchida, S

    Grassland Science   44   199 - 203   1998年

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  • Effects of extraction and reconstitution of ryegrass juice on fermentation, digestion and in situ degradation of pressed cake silage 査読

    Naoki Nishino, Keiko Miyase, Mitsuaki Ohshima, Hiro-Omi Yokota

    Journal of the Science of Food and Agriculture   75 ( 2 )   161 - 166   1997年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Primary growth of Italian ryegrass was fractionated into green juice and pressed cake (PC). PC was ensiled either directly or after reconstitution with the juice which was spray dried and then added to water before ensiling (RPC). This process was aimed at distinguishing between chemical (removal of nutrients) and physical (mechanical disintegration) effects of fractionation. Two additional wilted silages were prepared from long cut or chopped Italian ryegrass (WL and WC, respectively). The fermentative quality, digestion and in situ degradation of dry matter for the four silages were compared. WC, RPC and PC silages indicated lactate-rich fermentation while WL silage had a comparatively low lactate content. PC silage had a lower (P &lt
    0.05) pH value with lower (P &lt
    0.05) amounts of organic acids compared with WC and RPC silage, suggesting that removal of buffering components with the juice had greater effects than shredding on fermentation of PC silage. The digestibility of protein was lower (P &lt
    0.05) in PC than WL silage but the reduction was compensated for in RPC silage. Ruminal concentrations of total volatile fatty acids were the highest (P &lt
    0.05) when goats fed WL silage while those on the other silages had similar concentrations. Ruminal pH was lower (P &lt
    0.05) in goats fed PC than WC silage and the difference was not fully reversed by reconstituting the juice to PC. Juice extraction decreased (P &lt
    0.05) the soluble fraction and the rate of disappearance of dry matter in the rumen but the potential degradability was not different among treatments. These results suggest that both chemical and physical changes due to the fractionation affect the characteristics of digestion of PC silage while the removal of nutrients has a major role in the modified silage fermentation.

    DOI: 10.1002/(SICI)1097-0010(199710)75:2<161::AID-JSFA854>3.0.CO;2-Q

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  • Ruminal and whole tract digestion of long cut, short cut and pressed cake hay prepared from Italian ryegrass by goats 査読

    Nishino, N, Miyase, K, Ohshima, M, Yokota, H

    Grassland Science   43   83 - 87   1997年

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  • Changes in nitrogenous compounds and rates of in situ N loss of soybean meal treated with sodium hydroxide or heat 査読

    N Nishino, Y Masaki, S Uchida

    Journal of Agricultural and Food Chemistry   44 ( 9 )   2667 - 2671   1996年9月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Rates of in situ N loss of soybean meal (SBM) were altered by various combinations of sodium hydroxide and heat treatment; some nitrogenous compounds involving the modified proteolysis were investigated. The amount of lysinoalanine (LAL), neutral detergent insoluble nitrogen, 1-fluoro-2,4-dinitrobenzene reactive lysine, and reducing power of SBM were linearly related (P &lt; 0.01) to rate of in situ N loss with correlation coefficients of -0.847, -0.835, 0.793, and -0.846, respectively. The results indicate that the LAL formation and the Maillard reaction are related to change in susceptibility of SBM protein to microbial degradation in the rumen.

    DOI: 10.1021/jf9602481

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  • Nitrogen utilization of goats fed silage with or without supplements having different rumen degradability 査読

    Nishino, N, Miyase, K, Ohshima, M, Yokota, H

    Grassland Science   41   202 - 206   1995年

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  • Formation of lysinoalanine following alkaline processing of soya bean meal in relation to the degradability of protein in the rumen 査読

    N. Nishino, S. Uchida, M. Ohshima

    Journal of the Science of Food and Agriculture   68 ( 1 )   59 - 64   1995年

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Effects of sodium hydroxide (NaOH) treatment on the formation of lysinoalanine (LAL) and on in‐situ and in‐vivo utilisation of soya bean meal (SBM) protein were investigated. Defatted SBM was sprayed with 0 (water), 25, 50 and 100 g NaOH (kg−1 dry matter), dried at 80°C for 2 h and then fed to four rumen cannulated goats in a 4 × 4 Latin square as a mixed diet (9: 1 w/w) of sudangrass hay and the SBM. LAL was not detected in non‐alkaline SBM, but the cross‐linked amino acid increased according to the level of NaOH addition. The maximum concentration of LAL was 1.26 g per 16 g nitrogen when the highest level of NaOH was applied. The NaOH treatment did not alter the digestibility or the retention of nitrogen of the mixed diet. Addition of NaOH at 25 and 50 g (kg−1 dry matter) lowered the solubility of protein, while the highest level of treatment had little effect. Lag time and rate of degradation of protein decreased due to the increasing level in NaOH treatment. A significant correlation (r = —0.860, P &lt
    0.01) between LAL content and rate of protein degradation suggested that the formation of cross‐linked amino acid would render protein more resistant to microbial degradation in the rumen. Copyright © 1995 John Wiley &amp
    Sons, Ltd

    DOI: 10.1002/jsfa.2740680110

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  • Influence of conservation method on the fiber digestion of Italian ryegrass by goats 査読

    Nishino, N, Wakita, Y, Uchida, S

    Animal Science and Technology   65   709 - 715   1994年8月

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    担当区分:筆頭著者  

    DOI: 10.2508/chikusan.65.709

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  • Effect of sodium hydroxide or ammonia treatment on digestion and rumen degradation of alfalfa silage in goats 査読

    Nishino, N, Uchida, S, Ohshima, M

    Journal of Japanese Society of Grassland Science   39   437 - 445   1994年

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  • A note on the fermentation characteristics and rumen degradation of low moisture alfalfa silage treated with sodium hydroxide or ammonia 査読

    N. Nishino, M. Ohshima, S. Uchida

    Animal Feed Science and Technology   47 ( 3-4 )   297 - 304   1994年

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The effects of alkali treatment on the fermentation characteristics and rumen degradation of low moisture alfalfa silage were investigated. First crop alfalfa was wilted and ensiled, either directly or after being sprayed with NaOH or NH3 solution (1.72% dry matter (DM)). The herbages were stored in laboratory silos and opened at 1, 2, 7, 14, 28 and 56 days after storage. Each NaOH and HN3 addition increased the pH value and delayed lactic acid production of the silage. Both alkali treatments decreased proteolysis during ensilage, and NaOH-treated silage showed the highest protein (hot water insoluble nitrogen) content. Nitrogen solubility in a mineral buffer solution was decreased by the alkali treatments, and NaOH-treated silage showed the lowest solubility. Degradation characteristics of DM and N in the rumen were determined by nylon bag incubation of 56th day silage samples with two rumen fistulated goats. The NaOH treatment significantly decreased the soluble N fraction (63.7%, 54.7% and 60.6% for untreated, NaOH-treated and NH3-treated silage, respectively) but did not influence the extent of degradation. The treatment tended to decrease the rate of N degradation. In contrast, the NH3 treatment significantly increased the rate of DM degradation (0.158 h-1, 0.151 h-1 and 0.182 h-1 for untreated, NaOH-treated and NH3-treated silage, respectively) without any great changes in the proportions of soluble and degradable fraction. © 1994.

    DOI: 10.1016/0377-8401(94)90132-5

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  • Ruminal degradation of alfalfa protein as influenced by sodium hydroxide and heat treatment 査読

    Nishino, N., Uchida, S., Ohshima, M.

    Animal Feed Science and Technology   48 ( 1-2 )   131 - 141   1994年

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Effects of sodium hydroxide (NaOH) and heat treatment of alfalfa on the degradation of protein in the rumen was investigated in relation to the changes of nitrogenous compounds. Fresh alfalfa was treated with 0, 1.16, 2.32 or 4.64 g NaOH per 100 g dry matter (DM) and then subjected to freeze-drying, or oven-drying for 24 h at 60 or 120°C. Both NaOH and heat treatment produced lysinoalanine at increasing rate with level of treatment to reach a maximum value of 238 mg per 100 g protein. Both treatments also increased neutral detergent insoluble nitrogen (NDIN) according to the increase in level of treatment. Acid detergent insoluble nitrogen (ADIN) was not affected by NaOH treatment but was significantly increased by heating at 120°C. Both NaOH and heat treatment modified protein into being less soluble and more slowly degradable in the rumen, but heating showed greater effects than NaOH treatment. The proportion of potentially degradable protein was little influenced by the treatments. Both NaOH and heat treatment decreased the rate of protein degradation, but significant differences were detected only with the highest level of treatment (4.64 g NaOH per 100 g DM or 120°C). Correlation coefficients of lysinoalanine, NDIN and ADIN content with rate of protein degradation in the rumen were -0.716, -0.950 and -0.834, respectively. These results suggest that lysinoalanine formation in protein by NaOH and heat treatment can make protein less susceptible to microbial degradation in the rumen, while protein-carbohydrate reaction enhanced the effects on protein degradation in this study. © 1994.

    DOI: 10.1016/0377-8401(94)90117-1

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  • Digestion of alkali-treated alfalfa silage by goats 査読

    Nishino, N, Miyase, K, Ohshima, M, Yokota, H

    Asian-Australasian Journal of Animal Sciences   6   5 - 11   1993年1月

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    担当区分:筆頭著者  

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  • Nutritive value of silages prepared from fiber and liquid residues after the separation of leaf nutrient concentrate from Italian ryegrass in goats 査読

    Reddy, G.U, Ohshima, M, Nishino, S

    Asian-Australasian Journal of Animal Sciences   5   249 - 256   1992年

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  • Effect of addition of intact or alkalized lucerne juice at ensiling on the nutritive value of rice straw silage 査読

    Nishino, N, Ohshima, M, Yokota, H

    Asian-Australasian Journal of Animal Sciences   5   487 - 494   1992年

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    担当区分:筆頭著者  

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  • Nutritive value of rice straw ensiled with intact or alkalized Italian ryegrass green juice 査読

    Nishino, N, Ohshima, M, Yokota, H

    Journal of Japanese Society of Grassland Science   37   203 - 212   1991年

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    担当区分:筆頭著者  

    researchmap

  • Ruminal acid concentrations of goats fed hays and silages prepared from Italian ryegrass and its pressed cake 査読

    Ohshima, M, Miyase, K, Nishino, N, Okajima, T, Yokota, H

    Asian-Australasian Journal of Animal Sciences   4   59 - 65   1991年

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▼全件表示

書籍等出版物

  • Multifunctional Grasslands in a Changing World

    Guangdong People's Publishing House  2008年 

     詳細を見る

  • Silage production and utilisation

    Wageningen Academic Publishers  2005年 

     詳細を見る

  • Silage production and utilisation

    Wageningen Academic Publishers  2005年 

     詳細を見る

  • 動物の飼料

    文永堂  2004年 

     詳細を見る

  • 草地科学実験・調査法

    (社)畜産技術協会  2004年 

     詳細を見る

  • Relationships among Coagulation Methods, Yields and Nutritive Values of LPC(共著)

    Green Vegetation Fractionation  1996年 

     詳細を見る

  • Effect of Physical and Chemical Properties of Processed Italian Ryegrass on the Chemical and Nutritional Qualities of Silages(共著)

    Leaf Processing and Fractionation  1994年 

     詳細を見る

▼全件表示

MISC

  • Principle of silage fermentation

    Dairy Journal   16 - 23   2012年

     詳細を見る

  • サイレージ発酵の原理

    西野直樹

    酪農ジャーナル(臨時増刊号)   16 - 23   2012年

     詳細を見る

  • Aerobic stability and instability of silages caused by bacteria.

    Nishino, N

    Proceedings of the 2nd International Symposium on Forage Quality and Conservation.   127 - 441   2011年

     詳細を見る

  • Ensiled total mixed ration: A non-conventional silage supporting animal production in Japan.

    Nishino, N

    Proceedings of the 2nd China-Japan-Korea Symposium on Grassland Agriculture and Animal Production,Chinese Society of Grassland Science, Lanzhou.   pp. 193-197.   2006年

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講演・口頭発表等

  • Blood metabolites and rumen and fecal microbiota of Jersey cows throughout a lactation period

    Yousofi Zabialla, Peter Kiiru Gathinji, Nuomin, Riyan Baek, Souta Ashida, Ayumi Miyake, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021年11月3日  ISAB Organizing Committee

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    記述言語:英語  

    開催地:Ho Chi Minh City and Okayama-shi   国名:日本国  

  • Changes in blood metabolites concentration and fecal microbiota of dairy calves before and after weaning

    Nuomin, Daohu Ao, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021年11月3日  ISAB Organizing Committee

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Ho Chi Minh City and Okayama-shi   国名:日本国  

  • Bacterial and fungal microbiota of guinea grass silage stored at moderate and high ambient temperatures with and without wilting

    Jianjian Hou, Naoki Nishino, Takeshi Tsuruta

    International Symposium on Animal Bioscience 2021  2021年11月3日  ISAB Organizing Committee

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Ho Chi Minh City and Okayama-shi   国名:日本国  

  • Changes in the blood metabolites concentration, milk composition, and milk microbiota of Holstein cows during a lactation period

    Peter Kiiru Gathinji, Karin Akada, Daohu Ao, Sayo Nibuno, Masumi Kanadani, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021年11月3日  ISAB Organizing Committee

     詳細を見る

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Ho Chi Minh City and Okayama-shi   国名:日本国  

  • The relationship between milk, udder skin, bedding, and fecal microbiota in a dairy farm

    Daohu Ao, Karin Akada, Peter Kiiru Gathinji, Sayo Nibuno, Masumi Kanadani, Takeshi Tsuruta Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021年11月3日  ISAB Organizing Committee

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Ho Chi Minh City and Okayama-shi   国名:日本国  

  • 次世代シーケンサー(NGS)解析から見えてきた乳汁細菌叢と環境細菌叢の関係 招待

    西野直樹

    第25回日本乳房炎研究会  2021年10月24日  日本乳房炎研究会

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:仙台市   国名:日本国  

  • バンカーサイロで調製したトウモロコシホールクロップサイレージの細菌叢と真菌叢

    Hou Jianjian・西野直樹

    日本畜産学会129回大会  2021年9月15日  日本畜産学会

     詳細を見る

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台市   国名:日本国  

  • 貯蔵温度による発酵TMRの細菌叢および真菌叢の変動

    Wali Ajmal・西野直樹

    2021年度日本草地学会新潟大会  2021年3月20日  日本草地学会

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:新潟市   国名:日本国  

  • 酢酸型発酵を示したギニアグラスサイレージの細菌叢と真菌叢

    Hou Jianjian・西野直樹

    2021年度日本草地学会新潟大会  2021年3月20日  日本草地学会

     詳細を見る

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:新潟市   国名:日本国  

  • 貯蔵温度による発酵TMRの発酵特性、好気的安定性および細菌フローラの変動

    関西畜産学会  2011年 

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  • Lactobacillus panisおよびLactobacillus frumentiを添加したイタリアンライグラスおよびトウモロコシサイレージの発酵特性と好気的安定性

    日本草地学会第67回発表会  2011年 

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  • 予乾および糖蜜を添加したギニアグラスサイレージの細菌フローラ

    日本草地学会第67回発表会  2011年 

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  • イタリアンライグラスおよびギニアグラスから調製したダイレクトカットおよび予乾サイレージの発酵特性と細菌フローラ

    日本草地学会第67回発表会  2011年 

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  • Aerobic stability and instability of silages caused by bacteria

    2nd International Symposium on Forage Quality and Conservation  2011年 

     詳細を見る

  • Effect of inoculation of Acetobacter pasteurianus at ensiling and after silo opening on fermentation products, aerobic stability, and bacterial community of whole crop corn silage.

    2nd International Symposium on Forage Quality and Conservation  2011年 

     詳細を見る

  • Non-plate methods for silage microorganisms identification

    2nd International Symposium on Forage Quality and Conservation  2011年 

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  • 国産粗飼料を利用した発酵TMRの品質調査

    関西畜産学会  2011年 

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  • Bacterial community of direct-cut and wilted guinea grass silage stored with and without molasses.

    2nd International Symposium on Forage Quality and Conservation  2011年 

     詳細を見る

  • トウモロコシサイレージの貯蔵および好気的変敗におけるAcetobacter pasteurianus の役割

    関西畜産学会  2011年 

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  • 乳酸菌および糖蜜を添加したアルファルファサイレージの発酵特性、好気的安定性および細菌フローラ

    関西畜産学会  2011年 

     詳細を見る

  • 無添加あるいは乳酸菌を添加したロールベールサイレージの発酵特性と細菌群集の均一性

    日本畜産学会第112回大会  2010年 

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  • トウモロコシサイレージの好気的変敗に対するAcetobacter pasteurianusの添加効果

    日本草地学会第66回発表会  2010年 

     詳細を見る

  • 発酵TMRの発酵特性と細菌群集の季節変動

    日本畜産学会第112回大会  2010年 

     詳細を見る

  • 国内で流通する発酵TMRの微生物フローラ-サワードウと共通する多様なヘテロ発酵型乳酸菌の存在-

    日本草地学会第66回発表会  2010年 

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  • Lactobacillus plantarumおよびLactobacillus brevisを添加したサイレージの微生物フローラ

    日本草地学会第66回発表会  2010年 

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  • イタリアンライグラスサイレージの貯蔵および好気的変敗過程における微生物フローラの変化

    日本草地学会第66回発表会  2010年 

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  • コントラクタが調製したトウモロコシサイレージの細菌群集

    日本畜産学会第112回大会  2010年 

     詳細を見る

  • Storage properties and bacterial communities of wilted guinea grass and rhodes grass silages

    日本畜産学会第110回大会  2009年 

     詳細を見る

  • イタリアンライグラスおよびトウモロコシサイレージの好気的変敗に対するPediococcus acidilacticiとLactobacillus brevisの抑制効果

    関西畜産学会第59回大会  2009年 

     詳細を見る

  • バンカーサイロから分離したAcetobacter pasteurianusの性質とトウモロコシサイレージにおける生残性

    関西畜産学会第59回大会  2009年 

     詳細を見る

  • Aerobic stability of Italian ryegrass and corn silages treated with combined inoculation of Pediococcus acidilactici and Lactobacillus brevis

    15th International Silage Conference  2009年 

     詳細を見る

  • Variations in bacterial community and survival of inoculated bacteria in bunker silos assessed by denaturing gradient gel electrophoresis profiles

    15th International Silage Conference  2009年 

     詳細を見る

  • A survey of bacteria community structure of commercial total mixed ration silage

    15th International Silage Conference  2009年 

     詳細を見る

  • Inoculation effects and survival during ensilage of lactic acid bacteria derived from plant feeds and calf feces

    15th International Silage Conference  2009年 

     詳細を見る

  • Microflora associated with ensiling of wilted guinea grass as determined by culture-dependent and culture-independent analyses

    15th International Silage Conference  2009年 

     詳細を見る

  • Assessment of wilted guinea grass silage stored in laboratory-scale and big bale silo

    15th International Silage Conference  2009年 

     詳細を見る

  • バンカーサイロにおける微生物分布の不均一性-1年以上貯蔵した無添加トウモロコシサイレージにおける調査事例-

    日本草地学会第65回発表会  2009年 

     詳細を見る

  • Occurrence and survival in whole crop corn silage of Acetobacter pasteurianus

    15th International Silage Conference  2009年 

     詳細を見る

  • 子牛糞便あるいは発酵飼料から分離した乳酸菌のトウモロコシサイレージにおける生残性

    日本草地学会第65回発表会  2009年 

     詳細を見る

  • Influence of bacterial inoculants and storage period on fermentation and bacterial community of wilted guinea grass silage

    日本草地学会第65回発表会  2009年 

     詳細を見る

  • Variations in bacterial communities in bunker silos managed by a contractor

    日本草地学会第65回発表会  2009年 

     詳細を見る

  • 国内で流通している発酵TMRの実態調査

    日本畜産学会第110回大会  2009年 

     詳細を見る

  • ビール粕を主体とする発酵TMRの好気的安定性に関わる微生物

    日本畜産学会第109回大会  2008年 

     詳細を見る

  • 開封後1週間空気にさらした発酵TMRの飼料価値

    日本草地学会第64回発表会  2008年 

     詳細を見る

  • Microbial composition and fermentation products of guinea grass silage as influenced by wilting and storage period.

    日本草地学会第64回発表会  2008年 

     詳細を見る

  • 飼料イネホールクロップサイレージのアルコール生成に対するLactobacillus buchneriの抑制効果

    日本草地学会第63回発表会  2007年 

     詳細を見る

  • 発酵生成物、生菌数およびDGGEバンドパターンからみたパウチサイロとロールベールサイロの類似性

    日本畜産学会第107回大会  2007年 

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  • ビール粕あるいはトウフ粕を主体とする発酵TMRの貯蔵特性と微生物群集の解析

    日本畜産学会第107回大会  2007年 

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  • 混合素材の違いによるTMRサイレージの発酵特性と好気的安定性の変化

    日本草地学会第61会発表会  2006年 

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  • 食品工場から排出される廃糖液の乳酸菌培養基材としての利用

    日本草地学会第62会発表会  2006年 

     詳細を見る

  • 食品副産物を多用したTMRサイレージの貯蔵性制御-PCR-DGGEによる微生物の群集解析-

    日本畜産学会第106会大会  2006年 

     詳細を見る

  • Ensiled total mixed ration: A non-conventional silage supporting animal production in Japan.

    2nd China-Japan-Korea Symposium on Grassland Agriculture and Animal Production  2006年 

     詳細を見る

  • イタリアンライグラスロールベールサイレージにおける微生物叢の不均一性

    日本草地学会第61会発表会  2006年 

     詳細を見る

  • L. buchneri、L. plantarum(畜草1号)あるいはFJLB(付着乳酸菌事前培養液)を添加したイネホールクロップサイレージの発酵特性

    関西畜産学会第55回大会  2005年 

     詳細を見る

  • 飼料イネホールクロップサイレージにおける2,3-ブタンジオールの生成頻度

    日本草地学会第60回発表会  2005年 

     詳細を見る

  • 飼料イネホールクロップサイレージにおけるアルコールの生成とLactobacillus buchneriによる抑制

    日本草地学会第60回発表会  2005年 

     詳細を見る

  • Lactobacillus buchneriを添加したトウモロコシホールクロップサイレージの乳牛における利用性

    日本畜産学会第104回大会  2005年 

     詳細を見る

  • 食品副産物を多用したTMRサイレージの貯蔵性制御-アルファルファの有無と空気注入による好気的安定性の変動-

    日本畜産学会第105回大会  2005年 

     詳細を見る

  • Lactobacillus buchneriを添加したトウモロコシホールクロップ及びTMRサイレージにおけるポリアミン生成量

    日本草地学会第59回発表会  2004年 

     詳細を見る

  • 緑茶殻のサイレージ調製過程におけるカテキン類の消長

    日本畜産学会第103回大会  2004年 

     詳細を見る

  • 飼料イネホールクロップサイレージの発酵特性とLactobacillus buchneriの添加効果

    関西畜産学会第54回大会  2004年 

     詳細を見る

  • 飼料イネホールクロップサイレージにおけるLactobacillus buchneriの添加効果

    日本草地学会第59回発表会  2004年 

     詳細を見る

  • 緑茶殻のサイレージ調製過程におけるカテキン類の消長

    日本畜産学会第103回大会  2004年 

     詳細を見る

  • 飼料イネホールクロップサイレージにおけるLactobacillus buchneriの添加効果

    日本草地学会第59回発表会  2004年 

     詳細を見る

  • Lactobacillus buchneriを添加したトウモロコシホールクロップ及びTMRサイレージにおけるポリアミン生成量

    日本草地学会第59回発表会  2004年 

     詳細を見る

  • ビール粕を多用したTMRサイレージの好気的安定性に関する一考察

    関西畜産学会第53回大会  2003年 

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  • 1,2-プロパンジオール生成菌Lactobacillus buchneriによる牧草サイレージの好気的変敗抑制

    日本畜産学会第101回大会  2003年 

     詳細を見る

  • ビール粕を主体とするTMR型サイレージの優れた好気的安定性について

    日本草地学会第58回発表会  2003年 

     詳細を見る

  • Evaluation of Lactobacillus buchneri derived from by-products ensiling as an inoculum for whole crop maize silage.

    13th International Silage Conference  2002年 

     詳細を見る

  • ヘテロ発酵型乳酸菌等によるサイレージ発酵と好気的変敗防止―1,2-プロパンジオール生成菌Lactobacillus buchneriの機能―

    日本草地学会大会小集会―サイレージ研究の課題と今後の展望―  2002年 

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  • 1,2-プロパンジオール生成菌Lactobacillus buchneriの好気的変敗抑制機能―イタリアンライグラスサイレージの発酵特性と好気的安定性の変化―

    関西畜産学会第52回大会  2002年 

     詳細を見る

  • 1,2-プロパンジオールを生成するヘテロ型乳酸菌Lactobacillus buchneriを添加したトウモロコシサイレージの発酵特性と好気的安定性

    日本草地学会第57回発表会  2002年 

     詳細を見る

  • TMR型混合サイレージから分離した1,2-プロパンジオール生成菌Lactobacillus buchneriのサイレージ添加剤としての特性

    日本畜産学会第100回大会  2002年 

     詳細を見る

  • トウモロコシサイレージ中に生じた1,2-プロパンジオールについて

    日本畜産学会第98回大会  2001年 

     詳細を見る

  • ビール粕と発泡酒粕の飼料特性の比較―製造会社,製造ロットおよび脱水の有無による化学成分の変動―

    日本草地学会第56回発表会  2001年 

     詳細を見る

  • サイレージから分離した1,2-プロパンジオール生成菌Lactobacillus buchneriの性質

    関西畜産学会第51回大会  2001年 

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  • ビール粕および発泡酒粕サイレージの発酵特性―マルトース添加―

    日本畜産学会第98回大会  2001年 

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▼全件表示

受賞

  • 研究奨励賞

    1999年   日本草地学会  

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    受賞国:日本国

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共同研究・競争的資金等の研究

  • ジャージー種によるA2牛乳及び乳製品の開発事業

    2021年06月 - 2022年03月

    日本中央競馬会  令和3年度日本中央競馬会畜産振興事業 

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    担当区分:研究代表者  資金種別:競争的資金

    配分額:3920000円 ( 直接経費:3300000円 、 間接経費:620000円 )

 

担当授業科目

  • Advances in Animal Science (2021年度) 後期  - 金7,金8

  • Introduction to Animal Science (2021年度) 第2学期  - 火5,火6

  • まきばの実習 (2021年度) 夏季集中  - その他

  • コース演習3 (2021年度) 1・2学期  - その他

  • コース演習4 (2021年度) 3・4学期  - その他

  • 動物栄養学 (2021年度) 3・4学期  - 水1,水2

  • 動物栄養学演習 (2021年度) 後期  - その他

  • 動物栄養学演習 (2021年度) 前期  - その他

  • 動物栄養学演習 (2021年度) 後期  - その他

  • 動物栄養学演習 (2021年度) 後期  - その他

  • 動物栄養学演習 (2021年度) 前期  - その他

  • 動物栄養学演習 (2021年度) 前期  - その他

  • 動物栄養学特論 (2021年度) 後期  - 火1,火2

  • 動物栄養機能学特論 (2021年度) 後期  - その他

  • 動物生産管理学 (2021年度) 1・2学期  - 月1,月2

  • 卒業論文 (2021年度) 1~4学期  - その他

  • 卒業論文 (2021年度) 1~4学期  - その他

  • 応用動物科学コース概論2 (2021年度) 第4学期  - 水5,水6

  • 応用動物科学コース概論2 (2021年度) 第4学期  - 水5~6

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 研究科目演習1 (2021年度) 1・2学期  - その他

  • 研究科目演習2 (2021年度) 3・4学期  - その他

  • 研究科目演習II (2021年度) 1・2学期  - その他

  • 農業生物学1 (2021年度) 第1学期  - 水5,水6

  • 農業生物学1 (2021年度) 第1学期  - 水5~6

  • Advances in Animal Science (2020年度) 後期  - 金7,金8

  • Introduction to Animal Science (2020年度) 第2学期  - 火5,火6

  • まきばの実習 (2020年度) 夏季集中  - その他

  • イノベーション概論 (2020年度) 後期  - 月7,月8,金7,金8

  • イノベーション概論 (2020年度) 後期  - 月7~8,金7~8

  • コース演習1 (2020年度) 1・2学期  - 水3

  • コース演習2 (2020年度) 3・4学期  - 水3

  • コース演習4 (2020年度) 3・4学期  - その他

  • 動物栄養学演習 (2020年度) 前期  - その他

  • 動物栄養学演習 (2020年度) 後期  - その他

  • 動物栄養学演習 (2020年度) 後期  - その他

  • 動物栄養学演習 (2020年度) 後期  - その他

  • 動物栄養学演習 (2020年度) 前期  - その他

  • 動物栄養学演習 (2020年度) 前期  - その他

  • 動物栄養学特論 (2020年度) 後期  - 火1,火2

  • 動物栄養学1 (2020年度) 第3学期  - 水1,水2

  • 動物栄養学2 (2020年度) 第4学期  - 水1,水2

  • 動物栄養機能学特論 (2020年度) 後期  - その他

  • 動物生産管理学1 (2020年度) 第1学期  - 月1,月2

  • 動物生産管理学2 (2020年度) 第2学期  - 月1,月2

  • 卒業論文 (2020年度) 1~4学期  - その他

  • 卒業論文 (2020年度) 1~4学期  - その他

  • 応用動物科学コース概論1 (2020年度) 第3学期  - 金5,金6

  • 応用動物科学コース概論1 (2020年度) 第3学期  - 金5,金6

  • 応用動物科学コース概論2 (2020年度) 第4学期  - 金5,金6

  • 応用動物科学コース概論2 (2020年度) 第4学期  - 金5,金6

  • 生物生産科学特別研究 (2020年度) 通年  - その他

  • 生物生産科学特別研究 (2020年度) 通年  - その他

  • 生物生産科学特論Ⅱ (2020年度) 夏季集中  - その他

  • 研究科目演習1 (2020年度) 1・2学期  - その他

  • 研究科目演習2 (2020年度) 3・4学期  - その他

  • 研究科目演習II (2020年度) 1・2学期  - その他

  • 農業生物学1 (2020年度) 第1学期  - 水3,水4

▼全件表示

 

学術貢献活動

  • Chair of International Symposium on Animal Bioscience 2021 国際学術貢献

    役割:企画立案・運営等

    ISAB Organizing Committee  2021年11月3日

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    種別:大会・シンポジウム等 

  • Editor-in-Chief of Grassland Science 国際学術貢献

    役割:企画立案・運営等, 審査・評価

    Grassland Science  2020年4月1日 - 現在

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    種別:学会・研究会等