Updated on 2026/02/08

写真a

 
NISHINO Naoki
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Professor
Position
Professor
Homepage
https://www.okayama-u.ac.jp/user/an/
External link

Degree

  • Ph.D. (Agricultural Science) ( Nagoya University )

Research Interests

  • 動物栄養学

  • 動物衛生学

  • 飼料学

  • 野生動物学

Research Areas

  • Life Science / Animal production science

Education

  • Nagoya University   農学研究科   畜産学専攻

    - 1990

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    Country： Japan

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  • Nagoya University   農学部   畜産学科

    - 1988

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    Country： Japan

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Research History

  • 岡山大学大学院環境生命自然科学研究科   教授

    2021.4

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  • 岡山大学環境生命科学研究科 教授

    2015.4 - 2021.3

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  • 岡山大学自然科学研究科 准教授

    2004.12 - 2015.3

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Professional Memberships

Committee Memberships

  • 岡山県農林水産総合センター畜産研究所評価委員会   評価委員  

    2021.4 - 2022.3   

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    Committee type：Municipal

 

Papers

  • Specific heat-killed lactic acid bacteria enhance mucosal aminopeptidase N activity in the small intestine of aged mice. Reviewed

    Tsuruta, T., Wakisaka, M., Watanabe, T., Nishijima, A., Ikeda, A., Teraoka, M., Wang, T., Chen, K., Nishino, N.

    International Journal of Molecular Sciences   26   5742 - 5742   2025.6

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Aminopeptidase N (APN), an enzyme expressed in the small intestinal mucosa, is involved in dietary protein digestion. Previous studies have shown that oral administration of fermented milk containing lactic acid bacteria (LAB) enhances mucosal APN activity in young mice. This study aimed to investigate whether LAB strains stimulate mucosal APN activity in aged mice and to evaluate its relevance to age-related changes in body composition. The underlying molecular mechanisms were also explored in vitro. Experiment 1: Aged C57BL/6J mice were fed diets supplemented with heat-killed LAB strains—Enterococcus faecalis OU-23 (EF), Leuconostoc mesenteroides OU-03 (LM), or Lactiplantibacillus plantarum SNK12 (LP). Compared to the aged Control group, the ileal APN activity was significantly higher in the LP group. LP administration also elevated serum Gla-osteocalcin levels and decreased serum CTX-1 levels. Experiment 2: IEC-6 cells were co-cultured with LP that had been treated with RNase, DNase, or lysozyme. APN activity was significantly lower in cells co-cultured with DNase- or lysozyme-treated LP compared to those co-cultured with untreated LP. A specific LAB strain may enhance mucosal APN activity in the aged intestine, potentially contributing to improved bone metabolism. This effect may be mediated by bacterial DNA and peptidoglycan.

    DOI： 10.3390/ijms26125742

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  • Unabsorbed fecal fat content correlates with a reduction of immunoglobulin A coating of gut bacteria in high-lard diet-fed mice. Reviewed International journal

    Katsumata, E., Tsuruta, T., Sonoyama, K., Yoshida, T., Sasaki, M., Teraoka, M., Wang, T., Nishino, N.

    Molecular Nutrition & Food Research   68   e2400078   2024.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    SCOPE: Immunoglobulin A (IgA) selectively coats gut bacteria and contributes to regulatory functions in gastrointestinal inflammation and glucose metabolism. Excess intake of lard leads to decrease in the IgA coating of gut bacteria, although the underlying mechanisms remain unknown. This study validates how unabsorbed fat derived from a high-lard diet in the gut affects the IgA coating of bacteria, as assessed in mouse models using three types of dietary fat (lard, medium-, and long-chain triglycerides [MLCTs], and medium-chain triglycerides [MCTs]) exhibiting different digestibilities. METHODS AND RESULTS: C57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA-coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard-fed mice, MLCT- and MCT-fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations. CONCLUSION: Unabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high-lard diet-fed mice.

    DOI： 10.1002/mnfr.202400078

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  • Cyclic oligosaccharide-induced modulation of immunoglobulin A reactivity to gut bacteria contributes to alterations in the bacterial community structure Reviewed

    Miyamoto, T., Tsuruta, T., Teraoka, M., Wang, T., Nishino, N.

    Nutrients   16   2824 - 2824   2024.8

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Immunoglobulin A (IgA) is a major gut antibody that coats commensal gut bacteria and contributes to shaping a stable gut bacterial composition. Although previous studies have shown that cyclic oligosaccharides, including cyclic nigerosyl-1,6-nigerose (CNN) and cyclodextrins (CDs, including αCD, βCD, and γCD), alter the gut bacterial composition, it remains unclear whether cyclic oligosaccharides modify the IgA coating of gut bacteria, which relates to cyclic oligosaccharide-induced alteration of the gut bacterial composition. To address this issue, mice were maintained for 12 weeks on diets containing CNN, αCD, βCD, or γCD; the animals’ feces were evaluated for their bacterial composition and the IgA coating index (ICI), a measure of the degree of IgA coating of bacteria. We observed that the intake of each cyclic oligosaccharide altered the gut bacterial composition, with changes in the ICI found at both the phylum and genus levels. The ICI for Bacillota, Lachnospiraceae NK4A136 group, UC Lachnospiraceae, and Tuzzerella were significantly and positively correlated with the relative abundance (RA) in total bacteria for these bacteria; in contrast, significant correlations were not seen for other phyla and genera. Our observations suggest that cyclic oligosaccharide-induced modulation of the IgA coating of gut bacteria may partly relate to changes in the community structure of the gut bacteria.

    DOI： 10.3390/nu16172824

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  • Variations in milk, udder skin, and fecal microbiota and their relationships with blood metabolites and milk composition in dairy cows. Reviewed

    AoDaohu, Akada, K., Gathinji, P.K., Nishino, N.

    Letters in Applied Microbiology   77   ovae014   2024.3

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  • Commercial concentrate supplementation in Phan Rang sheep diets: Effects on digestibility traits, growth and carcass performance. Reviewed

    Nguyen, H.V., Nguyen, T.M., Dinh, V.D., Van, N.P., Tran, N.L., Le, T.H., Le, D.T., Vo, T.M., Ngo, M.D., Bui, V.L., Nguyen, X.B., Ton, N.M.T., Nishino, N.

    Advances of Animal and Veterinary Sciences   12   1 - 8   2024.1

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  • In vitro assessment of the relationships between the digestion of different types of rice straw and bacterial community in the rumen. Reviewed

    68   372 - 382   2023.9

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  • Cyclic nigerosylnigerose attenuates high-fat diet induced fa;dposition;colonic inflammation;d;abnormal;glucose metabolism;modifies gut immunoglobulin A reactivity to commensal bacteria. Reviewed International journal

    Tsuruta, T, Sonoyama, K, Miyamoto, T, Nguyen, Q.D, Mizote, A, Sasou, H, Shiotsu, M, Teraoka, M, Nishino, N

    Molecular Nutrition and Food Research   67   202200389   2023.7

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    SCOPE: High-fat diet (HFD) intake induces gut dysbiosis, inflammation in the peripheral tissues, and a reduction in immunoglobulin A (IgA) coating of gut bacteria, which is related to HFD-induced insulin resistance (IR). This study evaluates the effect of cyclic nigerosylnigerose (CNN), a dietary fiber that prevents gut inflammation and promotes IgA coating of gut bacteria, on the above-mentioned HFD-induced disorders. METHODS AND RESULTS: Balb/c mice are fed an HFD and administered CNN for 20 weeks. CNN administration reduces mesenteric adipose tissue weight, colonic tumor necrosis factor α (TNFα) mRNA expression, and serum endotoxin levels and ameliorates HFD-induced abnormal glucose metabolism. Additionally, CNN administration promotes gut bacteria-specific IgA secretion and alters IgA reactivity to gut bacteria. The alterations of IgA reactivity to specific bacteria such as Erysipelatoclostridium, Escherichia, Faecalibaculum, Lachnospiraceae genera, and Stenotrophomonas are correlated with mesenteric adipose tissue weight, colonic TNFα mRNA expression, serum endotoxin levels, and a homeostasis model assessment for IR. CONCLUSION: CNN-induced alterations in IgA reactivity to gut bacteria may be related to the suppression of HFD-induced fat deposition, colonic inflammation, endotoxemia, and IR. These observations indicate that dietary fiber that modulates IgA reactivity to gut bacteria may be useful in preventing HFD-induced disorders.

    DOI： 10.1002/mnfr.202200389

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  • Modulatory effects of A1 milk, A2 milk, soy, and egg proteins on gut microbiota and fermentation. Reviewed

    Nuomin, Baek, R, Tsuruta, T, Nishino, N

    Microorganisms   11   1194   2023.5

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  • Monitoring the milk composition, milk microbiota, and blood metabolites of Jersey cows throughout a lactation period. Reviewed

    Gathinji, P.K., Yousofi, Z., Akada, K., Wali, A., Nishino, N.

    Veterinary Sciences   10   226   2023.3

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  • Frequency of β-casein gene polymorphisms in Jersey cows in western Japan. Reviewed

    Nuomin, Nguyen, D.Q, Aodaohu, Nishino, N

    Animals   12   2076   2022.8

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  • Bacterial and fungal microbiota of total mixed ration silage stored at various temperatures. Reviewed International journal

    Wali, A, Hou, J, Tsuruta, T, Nishino, N

    Journal of Applied Microbiology   133 ( 2 )   579 - 590   2022.8

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    AIMS: To obtain insights into how bacterial and fungal microbiota and fermentation products composition are affected by storage temperature for TMR silage, which can be manufactured year-round. METHODS AND RESULTS: TMR silage was stored at 10°C, 25°C, ambient temperature (AT; 20-35°C) and 40°C. Lactic acid production was delayed when stored at 10°C, and acid production stagnated after 2 weeks when stored at 40°C. The patterns of acetic acid and ethanol production were inversely related, with ethanol production promoted at 10°C and 25°C and acetic acid production promoted at AT and 40°C. The bacterial diversity was reduced in TMR silage with high lactic acid and acetic acid content, and the fungal diversity was reduced in TMR silage with high ethanol content. CONCLUSIONS: The intensity of lactic acid production was accounted for by the high abundance of Lactobacillus, and its stagnated production at a substantially high storage temperature was related to an increased abundance of Bacillus. The enhanced production of acetic acid or ethanol can be explained by differences in the fungal microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY: The integrated analysis of bacterial and fungal microbiota can provide in-depth insights into the impact of storage temperature on TMR silage fermentation.

    DOI： 10.1111/jam.15582

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  • Fecal, milk, uterine, airborne dust, and water microbiota in dairy farms in southern Vietnam: A pilot study. Reviewed

    Tran, T.M.T, Tran, H.D, Nguyen, T.T, Acosta, T.J, Tsuruta, T, Nishino, N, Duong, T.H

    Advances of Animal and Veterinary Sciences   10 ( 7 )   1525 - 1531   2022.7

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    DOI： 10.17582/journal.aavs/2022/10.7.1525.1531

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  • Bacterial and fungal microbiota of guinea grass silage shows various levels of acetic acid fermentation Reviewed

    Hou, J, Nishino, N

    Fermentation   8   10   2022.1

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  • Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice Reviewed

    Tsuruta, T, Muhomah, T.A, Sonoyama, K, Nguyen, Q.D, Takase, Y, Nishijima, A, Himoto, S, Katsumata, E, Nishino, N

    Nutrition Research   93   15 - 26   2021.9

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.nutres.2021.06.009

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  • Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides Reviewed International journal

    Sivixay, S, Bai, G, Tsuruta, T, Nishino, N

    AIMS Microbiology   7 ( 1 )   1 - 12   2021.1

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Diet is considered the most influential factor in modulating the gut microbiota but how dietary protein sources differ in their modulatory effects is not well understood. In this study, soy, meat (mixture of beef and pork), and fish proteins (experiment 1) and soy, milk (casein), and egg proteins (experiment 2) were fed to rats with cellulose (CEL) and raffinose (RAF); the microbiota composition and short-chain fatty acid concentration in the cecum were determined. Egg protein feeding decreased the concentration of acetic acid and the richness and diversity of the cecum microbiota. RAF feeding increased the concentrations of acetic and propionic acids and decreased the richness and diversity of the cecum microbiota. When fed with CEL, the abundance of Ruminococcaceae and Christensenellaceae, Akkermansiaceae and Tannerellaceae, and Erysipelotrichaceae enhanced with soy protein, meat and fish proteins, and egg protein, respectively. The effects of dietary proteins diminished with RAF feeding and the abundance of Bifidobacteriaceae, Erysipelotrichaceae, and Lachnospiraceae increased and that of Ruminococcaceae and Christensenellaceae decreased regardless of the protein source. These results indicate that, although the effect of prebiotics is more robust and distinctive, dietary protein sources may influence the composition and metabolic activities of the gut microbiota. The stimulatory effects of soy, meat, and egg proteins on Christensenellaceae, Akkermansiaceae, and Erysipelotrichaceae deserve further examination to better elucidate the dietary manipulation of the gut microbiota.

    DOI： 10.3934/microbiol.2021001

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  • An investigation of seasonal variations in the microbiota of milk, feces, bedding, and airborne dust Reviewed

    Nguyen, T.T, Wu, H, Nishino, N

    Asian-Australasian Journal of Animal Sciences   33 ( 11 )   1858 - 1865   2020.11

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  • Bacterial and fungal microbiota associated with the ensiling of wet soybean curd residue under prompt and delayed sealing conditions Reviewed

    Wali, A, Nishino, N

    Microorganisms   8   9   2020.9

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  • Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice Reviewed

    Tsuruta, T, Katsumata, E, Mizote, A, Hou, J, Muhomah, T.A, Nishino, N

    Bioscience of Microbiota, Food and Health   39 ( 3 )   188 - 196   2020.7

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    Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.

    DOI： 10.12938/bmfh.2020-012

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  • Examination of milk microbiota, fecal microbiota, and blood metabolites of Jersey cows in cool and hot seasons Reviewed International journal

    Nguyen, Q.D, Tsuruta, T, Nishino, N

    Animal Science Journal   91 ( 1 )   e13441   2020.1

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    Microbiota of individual cow milk, bulk tank milk, and feces of Jersey cows were examined. Samples were collected from two farms (F1 and F2) in cool (November, Nov) and hot (July, Jul) seasons. Milk yield and milk composition were similar between the two farms and between the two seasons. Prevalent taxa of the fecal microbiota, i.e. Ruminococcaceae, Bacteroidaceae, Lachnospiraceae, Rikenellaceae, and Clostridiaceae, were unaffected by the farm and season. Relative abundance of milk microbiota for Pseudomonadaceae, Enterobacteriaceae, and Streptococcaceae (F1 > F2) and Lactobacillaceae, Bifidobacteriaceae, and Cellulomonadaceae (F1 < F2) were different between the two farms, and those for Staphylococcaceae, Bacillaceae, Ruminococcaceae, and Veillonellaceae (Nov < Jul) and Methylobacteriaceae and Moraxellaceae (Nov > Jul) were different between the two seasons. The microbiota of bulk tank milk was numerically different from that of individual cow milk. Principal coordinate analysis indicated that the milk microbiota was unrelated to the fecal microbiota. The finding that relative abundance of Pseudomonadaceae and Moraxellaceae appeared greater than those reported for Holstein milk suggested that higher protein and fat content may result in a greater abundance of proteolytic and lipolytic taxa in Jersey cow milk.

    DOI： 10.1111/asj.13441

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  • The relationship between uterine, fecal, bedding, and airborne dust microbiota from dairy cows and their environment: A pilot study Reviewed

    Nguyen, T.T, Miyake, A, Tran, T.T.M, Tsuruta, T, Nishino, N

    Animals   9 ( 12 )   1007   2019.12

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  • High-fat diet reduces the level of secretory immunoglobulin A coating of commensal gut microbiota Reviewed

    Muhomah, T.A, Nishino, N, Katsumata, E, Wu, H, Tsuruta, T

    Bioscience of Microbiota, Food and Health   38 ( 2 )   55 - 64   2019.4

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  • Rumen fluid, feces, milk, water, feed, airborne dust, and bedding microbiota in dairy farms managed by automatic milking systems Reviewed

    Wu, H, Nguyen, Q.D, Tran, T.T.M, Tang, M.T, Tsuruta, T, Nishino, N

    Animal Science Journal   90 ( 3 )   445 - 452   2019.3

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  • Dietary soy, meat, and fish proteins modulate the effects of prebiotic raffinose on composition and fermentation of gut microbiota in rats Reviewed

    Gaowa Bai, Takeshi Tsuruta, Naoki Nishino

    International Journal of Food Sciences and Nutrition   69 ( 4 )   480 - 487   2018.5

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Taylor and Francis Ltd  

    DOI： 10.1080/09637486.2017.1382454

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  • Effects of wilting and lactic acid bacteria inoculation on fermentation and microbial community of elephant grass silage produced in Vietnam Reviewed

    Thi Minh Tu Tran, Mui Thi Nguyen, Huu Van Nguyen, Naoki Nishino

    Grassland Science   64 ( 2 )   151 - 155   2018.4

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing Inc.  

    DOI： 10.1111/grs.12187

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  • Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut Reviewed

    Hongyan Han, Chao Wang, Yanbing Li, Zhu Yu, Qingfang Xu, Guangpeng Li, Tang Thuy Minh, Naoki Nishino

    Animal Science Journal   89 ( 1 )   97 - 104   2018.1

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing  

    DOI： 10.1111/asj.12896

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  • Variability, stability, and resilience of fecal microbiota in dairy cows fed whole crop corn silage Reviewed

    Minh Thuy Tang, Hongyan Han, Zhu Yu, Takeshi Tsuruta, Naoki Nishino

    Applied Microbiology and Biotechnology   101 ( 16 )   6355 - 6364   2017.8

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    DOI： 10.1007/s00253-017-8348-8

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  • Comparative microbiota assessment of wilted Italian ryegrass, whole crop corn, and wilted alfalfa silage using denaturing gradient gel electrophoresis and next-generation sequencing Reviewed

    Kuikui Ni, Tang Thuy Minh, Tran Thi Minh Tu, Takeshi Tsuruta, Huili Pang, Naoki Nishino

    Applied Microbiology and Biotechnology   101 ( 4 )   1385 - 1394   2017.2

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    DOI： 10.1007/s00253-016-7900-2

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  • Dietary casein and soy protein isolate modulate the effects of raffinose and fructooligosaccharides on the composition and fermentation of gut microbiota in rats Reviewed

    Gaowa Bai, Kuikui Ni, Takeshi Tsuruta, Naoki Nishino

    Journal of Food Science   81 ( 8 )   H2093 - H2098   2016.8

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    DOI： 10.1111/1750-3841.13391

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  • Lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration Reviewed

    Li, Y., Wang, F., Nishino, N.

    Asian-Australasian Journal of Animal Sciences   29 ( 4 )   516 - 522   2016.4

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    DOI： 10.5713/ajas.15.0267

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  • Identification and isolation of Lactobacillus fructivorans from wilted alfalfa silage with and without molasses Reviewed

    B. Wu, N. Nishino

    Journal of Applied Microbiology   120 ( 3 )   543 - 551   2016.3

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    DOI： 10.1111/jam.13031

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  • Fermentation and microbial population dynamics during the ensiling of native grass and subsequent exposure to air Reviewed

    Qing Zhang, Baiyila Wu, Naoki Nishino, Xianguo Wang, Zhu Yu

    Animal Science Journal   87 ( 3 )   389 - 397   2016.3

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    DOI： 10.1111/asj.12427

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  • Identification of bacteria in total mixed ration silage produced with and without crop silage as an ingredient Reviewed

    Naoki Nishino, Yu Ogata, Hongyan Han, Yasunari Yamamoto

    Animal Science Journal   86 ( 1 )   45 - 50   2015.1

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    DOI： 10.1111/asj.12234

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  • Bacterial communities in alfalfa and corn silages produced in large-scale stack and bunker silos in China Reviewed

    Baiyila Wu, Qing Zhang, Zhongkuan Liu, Zhu Yu, Naoki Nishino

    Grassland Science   60 ( 4 )   247 - 251   2014.12

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    DOI： 10.1111/grs.12063

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  • A pilot examination of the fermentation products, aerobic stability and bacterial community of total mixed ration silage produced in Vietnam Reviewed

    Tu Tran Thi Minh, Van Nguyen Huu, Naoki Nishino

    Grassland Science   60 ( 1 )   63 - 68   2014.3

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    DOI： 10.1111/grs.12041

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  • A survey of fermentation products and bacterial communities in corn silage produced in a bunker silo in China Reviewed

    Chao Wang, Hongyan Han, Xueying Gu, Zhu Yu, Naoki Nishino

    Animal Science Journal   85 ( 1 )   32 - 36   2014.1

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    DOI： 10.1111/asj.12076

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  • Effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content Reviewed

    Yanbing Li, Naoki Nishino

    Asian-Australasian Journal of Animal Sciences   26 ( 9 )   1304 - 1312   2013.9

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    DOI： 10.5713/ajas.2013.13175

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  • Changes in the bacterial community and composition of fermentation products during ensiling of wilted Italian ryegrass and wilted guinea grass silages Reviewed

    Yanbing Li, Naoki Nishino

    Animal Science Journal   84 ( 8 )   607 - 612   2013.8

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    DOI： 10.1111/asj.12046

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  • Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri on fermentation, aerobic stability and microbial communities in whole crop corn silage Reviewed

    Yanbing Li, Naoki Nishino

    Grassland Science   57 ( 4 )   184 - 191   2011.12

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1744-697X.2011.00226.x

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  • Monitoring the bacterial community of maize silage stored in a bunker silo inoculated with Enterococcus faecium, Lactobacillus plantarum and Lactobacillus buchneri Reviewed

    Y. Li, N. Nishino

    Journal of Applied Microbiology   110 ( 6 )   1561 - 1570   2011.6

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    DOI： 10.1111/j.1365-2672.2011.05010.x

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  • Chemical Changes during Ensilage and In sacco Degradation of Two Tropical Grasses: Rhodesgrass and Guineagrass Treated with Cell Wall-degrading Enzymes Reviewed

    Yu Zhu, Naoki Nishino, Guo Xusheng

    Asian-Australasian Journal of Animal Sciences   24 ( 2 )   214 - 221   2011.2

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  • Presence of sourdough lactic acid bacteria in commercial total mixed ration silage as revealed by denaturing gradient gel electrophoresis analysis

    C. Wang, N. Nishino

    LETTERS IN APPLIED MICROBIOLOGY   51 ( 4 )   436 - 442   2010.10

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    DOI： 10.1111/j.1472-765X.2010.02915.x

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  • Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and rhodes grass silages

    S. Parvin, C. Wang, Y. Li, N. Nishino

    ANIMAL FEED SCIENCE AND TECHNOLOGY   160 ( 3-4 )   160 - 166   2010.9

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    DOI： 10.1016/j.anifeedsci.2010.07.010

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  • Succession of lactic acid bacteria in wilted rhodesgrass silage assessed by plate culture and denaturing gradient gel electrophoresis Reviewed

    Suraya Parvin, Naoki Nishino

    GRASSLAND SCIENCE   56 ( 1 )   51 - 55   2010.3

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    DOI： 10.1111/j.1744-697X.2009.00173.x

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  • Bacterial community associated with ensilage process of wilted guinea grass Reviewed

    S. Parvin, N. Nishino

    JOURNAL OF APPLIED MICROBIOLOGY   107 ( 6 )   2029 - 2036   2009.12

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    DOI： 10.1111/j.1365-2672.2009.04391.x

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  • Association of Lactobacillus buchneri with aerobic stability of total mixed ration containing wet brewers grains preserved as a silage Reviewed

    Fujin Wang, Naoki Nishino

    ANIMAL FEED SCIENCE AND TECHNOLOGY   149 ( 3-4 )   265 - 274   2009.3

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    DOI： 10.1016/j.anifeedsci.2008.06.012

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  • Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration Reviewed

    F. Wang, N. Nishino

    Journal of Dairy Science   91 ( 6 )   2380 - 2387   2008.6

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    DOI： 10.3168/jds.2007-0821

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  • Variations in bacterial communities in laboratory-scale and big bale silos assessed by fermentation products, colony counts and denaturing gradient gel electrophoresis profiles Reviewed

    Nishino N, Tanabe Y

    Letters in Applied Microbiology   46 ( 3 )   283 - 288   2008.3

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    DOI： 10.1111/j.1472-765X.2007.02306.x

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  • Resistance to aerobic deterioration of total mixed ration silage: effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability Reviewed

    Fujin Wang, Naoki Nishino

    Journal of the Science of Food and Agriculture   88 ( 1 )   133 - 140   2008.1

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    DOI： 10.1002/jsfa.3057

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  • Effect of aerobic exposure after silo opening on feed intake and digestibility of total mixed ration silage containing wet brewers grains or soybean curd residue Reviewed

    WANG Fujin, NISHINO Naoki

    Grassland science   54 ( 3 )   164 - 166   2008

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    DOI： 10.1111/j.1744-697X.2008.00118.x

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  • Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria Reviewed

    Naoki Nishino, Hirotaka Hattori

    Journal of the Science of Food and Agriculture   87 ( 13 )   2420 - 2426   2007.10

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    DOI： 10.1002/jsfa.2911

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  • Biogenic amine production in grass, maize and total mixed ration silages inoculated with Lactobacillus casei or Lactobacillus buchneri Reviewed

    N. Nishino, H. Hattori, H. Wada, E. Touno

    Journal of Applied Microbiology   103 ( 2 )   325 - 332   2007.8

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    DOI： 10.1111/j.1365-2672.2006.03244.x

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  • Changes during ensilage in fermentation products, tea catechins, antioxidative activity and in vitro gas production of green tea waste stored with or without dried beet pulp Reviewed

    Naoki Nishino, Tetsuya Kawai, Makoto Kondo

    Journal of the Science of Food and Agriculture   87 ( 9 )   1639 - 1644   2007.7

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    DOI： 10.1002/jsfa.2842

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  • Alcoholic fermentation and its prevention by Lactobacillus buchneri in whole crop rice silage Reviewed

    N. Nishino, H. Hattori, Y. Kishida

    Letters in Applied Microbiology   44 ( 5 )   538 - 543   2007.5

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    DOI： 10.1111/j.1472-765X.2006.02105.x

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  • Ethanol and 2,3-butanediol production in whole-crop rice silage. Reviewed

    Nishino, N, Shinde, S

    Grassland Science   53 ( 3 )   196 - 198   2007

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    DOI： 10.1111/j.1744-697X.2007.00089.x

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  • Potential nutritive value of selected browse species from Kenya using in vitro gas production technique and polyethylene glycol. Reviewed

    Osuga, I. M, Abdulrazak, S. A, Nishino, N, Ichinohe, T, Fujihara, T

    Livestock Research for Rural Development   2006

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  • Ensiling characteristics and aerobic stability of direct-cut and wilted grass silages inoculated with Lactobacillus casei or Lactobacillus buchneri Reviewed

    N Nishino, E Touno

    Journal of the Science of Food and Agriculture   85 ( 11 )   1882 - 1888   2005.8

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    DOI： 10.1002/jsfa.2189

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  • Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri Reviewed

    N Nishino, H Wada, M Yoshida, H Shiota

    Journal of Dairy Science   87 ( 8 )   2563 - 2570   2004.8

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    DOI： 10.3168/jds.S0022-0302(04)73381-0

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  • Enhanced lactic acid fermentation of silage by the addition of green tea waste Reviewed

    M Kondo, N Naoki, K Kazumi, H Yokota

    Journal of the Science of Food and Agriculture   84 ( 7 )   728 - 734   2004.5

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    DOI： 10.1002/jsfa.1726

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  • Evaluation of fermentation and aerobic stability of wet brewers' grains ensiled alone or in combination with various feeds as a total mixed ration Reviewed

    N Nishino, H Harada, E Sakaguchi

    Journal of the Science of Food and Agriculture   83 ( 6 )   557 - 563   2003.5

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    DOI： 10.1002/jsfa.1395

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  • Changes in Soluble Sugars and Fermentation Products of TMR Silages Prepared with High and Low Malt Type Brewer's Grains Reviewed

    NISHINO Naoki, HARADA Hiroaki, SAKAGUCHI Ei

    Japanese Journal of Grassland Science   49 ( 4 )   367 - 372   2003

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    Two types of brewer's grains (BG), derived from high and low malt brewing, were mixed with various dry feeds (BG : beet pulp : oats hay : wheat bran : molasses=5 : 2 : 1 : 1 : 1 on a fresh weight basis), and ensiled in polyethylene bottles to prepare a total mixed ration (TMR silage). Silos were opened after 5, 20 and 40 d of storage ; the composition of soluble sugars and the profile of fermentation products were determined. Experiment was duplicated using different lots of BG (Exp. 1 & 2). Regardless of the type of BG, lactic acid production occurred and large numbers of lactic acid bacteria were found at 12 h after BG production. Principal sugars were maltose and raffinose in both BG, whilst the amount was higher in low malt type BG. When TMR silage was made, the main sugars altered to fructose, glucose and sucrose due to added feeds, and the differences in sugar contents diminished. Lactic acid (Exp. 1) or ethanol (Exp. 2) was the major fermentation product in TMR silage, and this property was not influenced by the type of BG used. Mannitol accumulated in lactate silages as storage prolonged, indicating that heterolactic fermentation was enhanced even when lactate silage was obtained. Accelerated ethanol production was found in ethanol silage from the beginning of storage ; the highest content (75gkg^<-1> DM) was given in TMR silage prepared with low malt type BG. Propionic and butyric acids were almost undetectable and ammonia N accounted for less than 60gkg^<-1> N in any of the silages, suggesting that TMR silage can be considered "well preserved" regardless of lactate and ethanol fermentation. Significant differences due to high and low malt type BG were found in the fermentation of TMR silage ; however, the production lot had much greater effects in the two experiments.

    DOI： 10.14941/grass.49.367

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  • Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri Reviewed

    N Nishino, M Yoshida, H Shiota, E Sakaguchi

    Journal of Applied Microbiology   94 ( 5 )   800 - 807   2003

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    DOI： 10.1046/j.1365-2672.2003.01810.x

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  • Content of phenolics and tannins in leaves and pods of some Acacia and Dichrostachys species and effects on in vitro digestibility Reviewed

    CDK Rubanza, M Shem, R Otsyina, N Nishino, T Ichinohe, T Fujihara

    Journal of Animal and Feed Sciences   12 ( 3 )   645 - 663   2003

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  • Response of intestinal proteinase activities to the feeding of isolated winged bean (Psophocarpus tetragonolobus) and soya bean (Glycine max) trypsin inhibitors in rats Reviewed

    N Nishino, M Taniguchi, E Sakaguchi

    Journal of the Science of Food and Agriculture   81 ( 11 )   1055 - 1059   2001.9

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    DOI： 10.1002/jsfa.891

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  • Disappearance of Maillard reaction products during ensilage and rumen fermentation in vitro Reviewed

    N Nishino, S Uchida

    Journal of the Science of Food and Agriculture   81 ( 2 )   275 - 280   2001.1

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    DOI： 10.1002/1097-0010(20010115)81:2<275::AID-JSFA814>3.0.CO;2-L

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  • Silage Making and Utilization of High Moisture By-products(Part 1) : 3. Ensiling Characteristics of Brewer's Grain Left after the Production of Beer and Happo-shu(Low Malt Beer)<Special Feature> Reviewed

    NISHINO Naoki, HARADA Hiroaki, SAKAGUCHI Ei

    Japanese Journal of Grassland Science   47 ( 3 )   318 - 322   2001

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    DOI： 10.14941/grass.47.318_1

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  • Fermentation of Rhodesgrass and Guineagrass Silages with or without Wilting and Added Cell Wall Degrading Enzymes Reviewed

    ZHU Yu, NISHINO Naoki, UCHIDA Senji

    Japanese Journal of Grassland Science   46 ( 1 )   22 - 27   2000

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    The effects of wilting and added enzymes on fermentation and in vitro digestion of rhodesgrass and guineagrass silages were examined. First cuts of the two forages were ensiled in laboratory silos directly or after being wilted for 4 h with or without cell wall degrading enzymes (0.2 g kg^<-1>). Silos were opened after 45 d and the chemical composition and the fermentation quality were determined. The silages were subjected to in vitro rumen incubation and the degradation of NDF was determined. Without enzymes rhodesgrass silage failed to achieve lactate type fermentation, while guineagrass silage had high proportion of lactate even without any of the treatments. Wilting inhibited the organic acid production but decreased the proportion of lactate in rhodesgrass silage. Use of enzymes improved the preservation of both the silages, as evidenced by the increase of lactate and the decrease of pH value and NH3-N. The contents of NDF and ADF were reduced by enzyme treatment, but the reduction was considerably suppressed when forages were wilted before ensiling. In vitro degradation of NDF was low in direct-cut rather than wilted silage. Addition of enzymes also lowered the degradation, but this effect was restrictedly found in guineagrass silage incubated for 72h. These results indicate that although added cell wall degrading enzymes can promise an improved fermentation of tropical grass silage, the benefit would be less pronounced when treated with low moisture material.

    DOI： 10.14941/grass.46.22_1

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  • Ensiling characteristics and ruminal degradation of Italian ryegrass and lucerne silages treated with cell wall-degrading enzymes Reviewed

    Y Zhu, N Nishino, Y Kishida, S Uchida

    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE   79 ( 14 )   1987 - 1992   1999.11

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    DOI： 10.1002/(SICI)1097-0010(199911)79:14<1987::AID-JSFA466>3.0.CO;2-J

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  • Laboratory evaluation of previously fermented juice as a fermentation stimulant for lucerne silage Reviewed

    N Nishino, S Uchida

    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE   79 ( 10 )   1285 - 1288   1999.7

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    DOI： 10.1002/(SICI)1097-0010(19990715)79:10<1285::AID-JSFA362>3.0.CO;2-M

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  • A note on the chemical changes associated with nonenzymatic browning of forages Reviewed

    Nishino, N, Touno, E, Uchida, S

    Grassland Science   44 ( 1 )   86 - 89   1998.1

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    DOI： 10.14941/grass.44.86_1

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  • Effects of cell wall degrading enzymes and lactic acid bacteria on the fermentation of rhodesgrass (Chloris gayana Kunth.) silage stored at various ambient temperature Reviewed

    NISHINO N

    Grassland Science   44   199 - 203   1998

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  • Effects of extraction and reconstitution of ryegrass juice on fermentation, digestion and in situ degradation of pressed cake silage Reviewed

    Naoki Nishino, Keiko Miyase, Mitsuaki Ohshima, Hiro-Omi Yokota

    Journal of the Science of Food and Agriculture   75 ( 2 )   161 - 166   1997.10

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    DOI： 10.1002/(SICI)1097-0010(199710)75:2<161::AID-JSFA854>3.0.CO;2-Q

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  • Ruminal and whole tract digestion of long cut, short cut and pressed cake hay prepared from Italian ryegrass by goats Reviewed

    Nishino, N, Miyase, K, Ohshima, M, Yokota, H

    Grassland Science   43 ( 2 )   83 - 87   1997

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    From a winter harvest of autumn-sown Italian ryegrass, long cut, short cut and pressed cake hay were prepared. The three hays were solely fed to goats equipped with rumen cannulae and the digestibility of nutrients, rumen fluid characteristics and in situ disappearance of dry matter were determined. The content of fibrous components increased during hay-making and the increased proportion was the highest in short cut hay and the lowest in pressed cake hay. The digestibility of pressed cake hay was similar to those of the other hays except for protein which was lower in pressed cake hay. The digestibilities of NDF and hemicellulose of short cut hay were significantly higher than those of long cut hay. Goats given pressed cake hay showed lower ruminal pH than those given short cut hay while maintaining less concentrations of volatile fatty acids after feeding. Ruminal degradation characteristics of dry matter revealed that short cut hay had less soluble fraction but higher rate of degradation compared with long cut hay. The pressing of macerated Italian ryegrass further decreased the soluble fraction but potential degradability was not lowered despite of the removal of highly digestible nutrients. The rate of degradation was similar between long cut and pressed cake hay. These results indicate that the chopping before hay-making would alter the characteristics of ruminal digestion of Italian ryegrass while not affecting the whole tract digestibility. Pressed cake hay was comparable to long cut hay in terms of digestibility and rate of degradation in the rumen.

    DOI： 10.14941/grass.43.83

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  • Changes in nitrogenous compounds and rates of in situ N loss of soybean meal treated with sodium hydroxide or heat Reviewed

    N Nishino, Y Masaki, S Uchida

    Journal of Agricultural and Food Chemistry   44 ( 9 )   2667 - 2671   1996.9

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    DOI： 10.1021/jf9602481

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  • Nitrogen utilization of goats fed silage with or without supplements having different rumen degradability Reviewed

    Nishino, N, Miyase, K, Ohshima, M, Yokota, H

    Grassland Science   41 ( 3 )   202 - 206   1995

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    Effects of feeding corn, barley and sugar beet pulp to silage based diet on the nitrogen (N) utilization of goats were studied. These supplements were selected due to having the difference in rumen degradation. Four rumen cannulated goats were fed the diets consisting of wilted ryegrass (Lolium multiftorum Lam) silage and the supplements (4 : 1 on dry matter (DM) basis). Silage was solely used for the control diet and N intake was adjusted by urea added to the diets including supplements. The wilted silage showed the pH value 4.72 and relatively high NH3-N concentration (16.7%/total-N). The soluble fraction of DM in the rumen was 58.0, 26.8 and -1.25%, and the rate of degradation was 0.042, 0.107 and 0.099h^<-1>, for corn, barley and sugar beet pulp, respectively. Dietary inclusion of corn significantly increased DM and organic matter digestibility, and that of barley increased hemicellulose digestibility compared with the control diet. Urinary excretion of allantoin increased when goats fed the supplements, but the difference failed to be significant. Ruminal concentration of NH_3-N and retention of N showed no significant difference among the treatments, but feeding barley tended to lose more N compared with the other supplements in this study.

    DOI： 10.14941/grass.41.202

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  • Formation of lysinoalanine following alkaline processing of soya bean meal in relation to the degradability of protein in the rumen Reviewed

    N. Nishino, S. Uchida, M. Ohshima

    Journal of the Science of Food and Agriculture   68 ( 1 )   59 - 64   1995

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    DOI： 10.1002/jsfa.2740680110

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  • Influence of conservation method on the fiber digestion of Italian ryegrass by goats Reviewed

    Nishino, N, Wakita, Y, Uchida, S

    Animal Science and Technology   65 ( 8 )   709 - 715   1994.8

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    Effect of preservation as hay and silage on the fiber digestion of Italian ryegrass was investigated. From the same harvest at heading stage, field dried hay and wilted silage were prepared. These forages were restrictedly fed to four rumen cannulated goats as a diet consisting of forage-soybean meal (9:1) mixture to determine nutrient digestibility, nitrogen balance, rumen degradability of DM and NDF, particle-bound carboxymethylcellulase (CMCase) activity, and rate of passage of liquid (Co-EDTA) and solid digesta (Cr-CWC). The wilted silage (DM 30.8%) showed a relatively high pH value 4.75 and a butyrate-rich fermentation. The hay showed higher NDF, NDIN and ADIN contents than silage, suggesting some protein became associated with fibrous components during hay-making. All the digestibility of silage diet was higher than those of hay diet, while significant difference could not be detected in fiber digestibility. When goats were fed silage, they retained more nitrogen than those on hay. The silage had more rumen soluble (5.5 and 0.0%) and less degradable (71.4 and 74.4%) NDF compared with hay. The rate of NDF degradation was lower in silage than hay (3.2 and 4.2%h-1), which may partly be ascribed to lower CMCase activity of silage. No significant difference was observed in the rate of passage of either liquid or feed particles. These results indicate that there is considerable difference as energy source in the fibrous components between hay and silage as well as a marked difference of soluble carbohydrate and volatile fatty acid contents.

    DOI： 10.2508/chikusan.65.709

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  • Effect of sodium hydroxide or ammonia treatment on digestion and rumen degradation of alfalfa silage in goats Reviewed

    Nishino, N, Uchida, S, Ohshima, M

    Journal of Japanese Society of Grassland Science   39 ( 4 )   437 - 445   1994

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    Three rumen cannulated goats were used to investigate the effect of NaOH and NH_3 treatment (2.74% DM) at ensiling on digestion, nitrogen utilization and in situ protein degradation of low moisture alfalfa silage. Wilted alfalfa was sprayed with water (control), NaOH or NH_3 solution, and fed to goats after 6 months storage in 3×3 Latin square design. Both alkali treatments increased the pH value, NH_3-N and butyric acid of the silage, but NH_3-treated silage preserved as much amino acids as control silage. The two alkali treatments could not improve digestibilities of fibrous components (NDF, ADF and cellulose), and NaOH addition significantly reduced the digestibility of crude protein. The NaOH treatment decreased the rate of N degradation in the rumen, while solubility and potential degradability was not affected by the treatment. The NH_3 addition increased ruminal soluble nitrogen in the silage, but rate of N degradation was similar to that of control silage. These results suggest that NaOH treatment would make protein less susceptible to microbial degradation in the rumen despite of deteriorating the fermentation quality of the silage.

    DOI： 10.14941/grass.39.437

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  • A note on the fermentation characteristics and rumen degradation of low moisture alfalfa silage treated with sodium hydroxide or ammonia Reviewed

    N. Nishino, M. Ohshima, S. Uchida

    Animal Feed Science and Technology   47 ( 3-4 )   297 - 304   1994

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    DOI： 10.1016/0377-8401(94)90132-5

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  • Ruminal degradation of alfalfa protein as influenced by sodium hydroxide and heat treatment Reviewed

    Nishino, N., Uchida, S., Ohshima, M.

    Animal Feed Science and Technology   48 ( 1-2 )   131 - 141   1994

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    DOI： 10.1016/0377-8401(94)90117-1

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  • Digestion of alkali-treated alfalfa silage by goats Reviewed

    Nishino, N, Miyase, K, Ohshima, M, Yokota, H

    Asian-Australasian Journal of Animal Sciences   6   5 - 11   1993.1

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  • Nutritive value of silages prepared from fiber and liquid residues after the separation of leaf nutrient concentrate from Italian ryegrass in goats Reviewed

    Reddy, G.U, Ohshima, M, Nishino, S

    Asian-Australasian Journal of Animal Sciences   5   249 - 256   1992

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  • Effect of addition of intact or alkalized lucerne juice at ensiling on the nutritive value of rice straw silage Reviewed

    Nishino, N, Ohshima, M, Yokota, H

    Asian-Australasian Journal of Animal Sciences   5   487 - 494   1992

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  • Nutritive value of rice straw ensiled with intact or alkalized Italian ryegrass green juice Reviewed

    Nishino, N, Ohshima, M, Yokota, H

    Journal of Japanese Society of Grassland Science   37 ( 2 )   203 - 212   1991

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    Freshly harvested Italian ryegrass was disintegrated with a herbage crusher and pressed out green juice (GJ) with a screw press. The GJ was used as a protein supplement source for rice straw (RS). Rice straw was mixed with intact GJ (GJ・RS) and with NaOH or NH_3 (4% of RS dry matter) treated GJ (GJ・NaOH・RS or GJ・NH_3・RS) at 3:7 ratio on fresh weight basis and ensiled into polyethylene bags. Rice straw ensiled with water was also prepared as the control (W・RS). Crude protein content of RS was increased from 5.0 to 7.9% in dry matter basis by mixing intact or NaOH treated GJ and further increased to over 25% by NH_3 treated GJ. The control W・RS silage contained a trace amount of lactic acid and showed a butyrate type fermentation. The addition of intact GJ to RS significantly increased the lactic acid content, and reduced the butyric acid content, NH_3-N/TN and the pH value of the silage. In contrast to this, the addition of NaOH or NH_3 treated GJ increased the pH value, the butyric acid content and NH_3-N/TN of the silage, and GJ・NH_3・RS silage showed the lowest chemical quality. In the feeding trial, four castrated male goats were allocated in a 4×4 Latin-square and fed the 3:1 (DM basis) mixed ration of RS silage and wheat bran. Besides them, urea was supplemented to W・RS silage at the time of feeding to adjust the nitrogen intake except for that from NH_3 treated silage. The addition of intact GJ to RS at ensiling had little advantage to feeding urea with W・RS silage in respect to nutrient digestibilities but slightly improved nitrogen balance of goats. Compared with goats fed W・RS and GJ・RS silages, those fed GJ・NaOH・RS silage showed significantly higher fiber digestibilities, less urinary nitrogen excretion and more nitrogen retention with the reduction of ruminal NH_3-N and blood urea nitrogen concentrations. The goats fed GJ・NH_3・RS silage showed the highest nutrient digestibilities and nitrogen retention. These results suggest that the alkali treatments increased energy supply from fiber and consequently increased nitrogen uptake of rumen microbes. The disadvantage of feeding GJ・NaOH・RS compared with GJ・NH_3・RS was probably due to the insufficient nitrogen supply to rumen microbes for the maximum fiber digestion and protein synthesis.

    DOI： 10.14941/grass.37.203

    CiNii Article

    CiNii Books

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    Other Link： http://agriknowledge.affrc.go.jp/RN/2010480737

  • Ruminal acid concentrations of goats fed hays and silages prepared from Italian ryegrass and its pressed cake Reviewed

    Ohshima, M, Miyase, K, Nishino, N, Okajima, T, Yokota, H

    Asian-Australasian Journal of Animal Sciences   4   59 - 65   1991

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Books

  • Multifunctional Grasslands in a Changing World

    Guangdong People's Publishing House  2008 

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  • Silage production and utilisation

    Wageningen Academic Publishers  2005 

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  • 草地科学実験・調査法

    （社）畜産技術協会  2004 

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  • 動物の飼料

    文永堂  2004 

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  • Relationships among Coagulation Methods, Yields and Nutritive Values of LPC(共著)

    Green Vegetation Fractionation  1996 

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  • Effect of Physical and Chemical Properties of Processed Italian Ryegrass on the Chemical and Nutritional Qualities of Silages（共著）

    Leaf Processing and Fractionation  1994 

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MISC

  • サイレージ発酵の原理

    西野直樹

    酪農ジャーナル（臨時増刊号）   16 - 23   2012

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  • Aerobic stability and instability of silages caused by bacteria.

    Nishino, N

    Proceedings of the 2nd International Symposium on Forage Quality and Conservation.   127 - 441   2011

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  • Ensiled total mixed ration : A non-conventional silage supporting animal production in Japan

    NISHINO N

    Proceedings of the 2nd China-Japan-Korea Symposium on Grassland Agriculture and Animal Production, 2006   pp. 193-197.   2006

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Presentations

  • Effect of cyclic nigerosylnigerose (CNN) on insulin resistance in high-fat diet-induced obese mice

    鶴田剛司, 溝手晶子, 塩津萌々子, 佐相晴香, 西野直樹

    日本農芸化学会大会講演要旨集(Web)  2021 

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    Event date： 2021

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  • 高脂肪食の摂取にともなう腸管IgAの腸内細菌に対する反応性の低下と肥満病態の関係性

    鶴田剛司, 園山慶, 西野直樹

    日本栄養・食糧学会大会講演要旨集  2020 

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    Event date： 2020

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  • 牛乳中の細胞外小胞が食餌誘導性肥満マウスの耐糖能異常に及ぼす影響

    樋本栞, 西野直樹, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集  2019 

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    Event date： 2019

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  • 牛乳中の細胞外小胞がAOM/DSS大腸がん誘導マウスの腫瘍形成に及ぼす影響

    椛島奈津美, 西野直樹, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集  2019 

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    Event date： 2019

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  • 牛乳に含まれる細胞外小胞がヒト大腸がん細胞株の増殖活性に及ぼす影響

    高瀬友里加, 鶴田剛司, 西野直樹

    日本栄養・食糧学会大会講演要旨集  2019 

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    Event date： 2019

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  • 岡山県美作市で捕獲された野生シカの血液性状と腸内細菌叢

    小田沙也可, 鶴田剛司, 西野直樹

    日本草地学会誌  2019 

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    Event date： 2019

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  • シクロニゲロシルニゲロース摂取がDSS腸炎に及ぼす影響

    勝又恵実子, 西野直樹, 中村修治, 角田省二, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集  2019 

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    Event date： 2019

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  • Reactivity of secretory immunoglobulin A against gut bacteria is attenuated by high-fat diet consumption

    鶴田剛司, MUHOMAH Teresia Aluoch, 園山慶, 西野直樹

    岡山実験動物研究会報  2019 

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    Event date： 2019

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  • シクロニゲロシルニゲロースの摂取が分泌型免疫グロブリンAの分泌および腸内細菌への反応性に及ぼす影響

    勝又恵実子, 西野直樹, TERESIA Muhomah, 鶴田剛司

    日本栄養・食糧学会大会講演要旨集  2018 

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    Event date： 2018

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞はマウスの内臓脂肪蓄積を抑制する

    伊藤洋平, 鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会中国・四国支部大会講演要旨集  2018 

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    Event date： 2018

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞は内臓脂肪組織の脂肪蓄積抑制を媒介する

    伊藤洋平, 鶴田剛司, 西野直樹, 園山慶

    日本栄養・食糧学会大会講演要旨集  2018 

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    Event date： 2018

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  • マクロファージが分泌する細胞外小胞は乳酸菌の抗肥満効果を媒介する

    伊藤洋平, 鶴田剛司, 西野直樹, MUHOMAH Terry, 園山慶

    岡山実験動物研究会報  2018 

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    Event date： 2018

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  • 分泌型免疫グロブリンAの腸内細菌への結合活性は高脂肪食の摂取により弱まる

    MUHOMAH Teresia Aluoch, 西野直樹, 園山慶, 桑高佳穂, 鶴田剛司

    日本栄養・食糧学会中国・四国支部大会講演要旨集  2018 

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    Event date： 2018

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞は細胞種選択的に細胞の栄養代謝に影響を及ぼす

    鶴田剛司, 伊藤洋平, MUHOMAH Terry, 西野直樹, 園山慶

    日本栄養・食糧学会大会講演要旨集  2017 

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    Event date： 2017

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  • DGGEおよびNGSによるイタリアンライグラス,アルファルファおよびトウモロコシサイレージの細菌叢解析

    西野直樹, NI Kuikui, YEN Ngoc Phan Thi, BAIYILA Wu, 鶴田剛司

    日本畜産学会大会講演要旨  2016 

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    Event date： 2016

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  • Lactobacillus plantarum No.14株を貪食したマクロファージが放出する細胞外小胞は白色脂肪細胞の脂肪蓄積を抑制する

    鶴田剛司, 伊藤洋平, 西野直樹, MUHOMAH Terry, 園山慶

    日本栄養・食糧学会中国・四国支部大会講演要旨集  2016 

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    Event date： 2016

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  • 食餌タンパク質およびオリゴ糖による盲腸内短鎖脂肪酸濃度と細菌フローラの変動

    白高娃, 鶴田剛司, 西野直樹

    日本栄養・食糧学会中国・四国支部大会講演要旨集  2015 

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    Event date： 2015

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  • 卵白タンパク質の腸内細菌叢修飾作用とリゾチームの関与

    池尾 遥, 清親拓真, Sounisa Thiengvannathy, 鶴田剛司, 西野直樹

    第75回関西畜産学会大会  2025.11.14 

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  • A1タイプとA2タイプならびにミルクとヨーグルトにおけるカゼインの機能的差異

    前原 彩音, 小口あゆむ, 前田貴一, 鶴田剛司, 西野直樹

    第75回関西畜産学会大会  2025.11.14 

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  • PepX活性の異なる乳酸菌スターターで調製したヨーグルトの特性

    小口あゆむ, 前田貴一, 前原 彩音, 鶴田剛司, 西野直樹

    第75回関西畜産学会大会  2025.11.14 

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  • Progress on forage conservation research in Japan Invited

    Nishino, N.

    International project meeting between China and Japan  2025.5.11 

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  • Jersey industry and related research in Japan Invited

    Naoki, N.

    International Symposium for the Promotion of Jersey Dairy Cattle Industry of Korea  2024.9.25 

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  • 十二指腸、回腸および腎臓におけるDPP4 活性の差異と食餌タンパク質による変動

    清親拓真, 白 里陽, 鶴田剛司, 西野直樹

    第73回関西畜産学会大会  2023.11.11 

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  • ジャージー種におけるCSN2およびCSN3遺伝子の多型と乳量および乳成分の関係

    前田貴一, Nuomin, 鶴田剛司, 西野直樹

    第73回関西畜産学会大会  2023.11.11 

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  • Digesta- and tissue-associated bacteria inhabiting various gut segments of goats determined by conventional and viability PCR

    Tran Dinh Phong, Takeshi Tsuruta, Naoki Nishino

    第73回関西畜産学会大会  2023.11.11 

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  • Effect of lard intake on immunoglobulin A coating of gut bacteria

    Mao Teraoka, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2023  2023.11.3 

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  • Dipeptidyl peptidase 4 activity in the duodenum, ileum, and liver of mice fed various dietary proteins

    Takuma Kiyochika, Riyan Baek, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2023  2023.11.3 

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  • Monitoring the digesta- and tissue-associated bacteria inhabiting various gut segments of goats using conventional and viability PCR

    Dinh Phong Tran, Ao Daohu, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2023  2023.11.3 

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  • Progress and outlook of silage microbiology research Invited

    Naoki Nishino

    2023 International Conference on Frontier in Grassland Science  2023.10.16 

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  • The effect of protein sources on gut microbiota in mice with and without osmotic diarrhea

    Nuomin, Baek Riyang, Tran Dinh Phong, Tonnu.Minh Thi, Tsuruta Takeshi, Nishino Naoki

    The 4th International Conference on Sustainable Agriculture and Environment  2022.11.18 

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  • β-casein gene polymorphism in Jersey cows reared in Okayama, Japan

    Aodaofu, Nuomin, Tran Dinh Phong, Li Dong Ryang, Maeda Takakazu, Nishino Naoki

    The 4th International Conference on Sustainable Agriculture and Environment  2022.11.18 

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  • Milk microbiota, milk composition, and blood metabolites of Jersey cows throughout a lactation period

    Gathinji Peter Kiiru, Zabiallah Yousofi, Nguyen Dang Qui, Aodaofu, Nishino Naoki

    The 4th International Conference on Sustainable Agriculture and Environment  2022.11.18 

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  • Inhibited aerobic spoilage by multiple lactic acid bacteria inhabiting total mixed ration silage

    Wali Ajmal, Hou Jianjian, Tran Dinh Phong, Tonnu Minh Thi, Nishino Naoki

    The 4th International Conference on Sustainable Agriculture and Environment  2022.11.18 

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  • Production of β-casomorphin from A1 milk, A2 milk, A1 yogurt, and A2 yogurt

    Baek Riyang, Nuomin, Tonnu Minh Thi, Kiyochika Takuma, Tsuruta Takeshi, Nishino Naoki

    The 4th International Conference on Sustainable Agriculture and Environment  2022.11.18 

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  • 岡山県蒜山地域におけるジャージー乳A2化の取組み Invited

    西野直樹

    第72回関西畜産学会  2022.10.15 

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  • A1型およびA2型の牛乳とヨーグルトから調製したカゼインの特性

    白里陽 Nuomin, 鶴田剛司, 西野直樹

    第72回関西畜産学会  2022.10.15 

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  • Comparison of the effect on gut microbiota between A1 and A2 caseins using mice

    Nuomin, Baek Riyang, Aodaofu, Tsuruta Takeshi, Nishino Naoki

    第72回関西畜産学会  2022.10.15 

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  • Microbiology of total mixed ration silage —A feed preservation supporting sustainable use of wet by-products— Invited

    Nishino Naoki

    International Conference on Sustainable Agriculture for Food Security  2022.6.24 

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  • 牧草類の嫌気的貯蔵に関わる細菌叢と真菌叢

    侯健健 Wali, Ajmal 西野直樹

    2022年度日本草地学会岩手大会  2022.3.25 

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  • Blood metabolites and rumen and fecal microbiota of Jersey cows throughout a lactation period

    Yousofi Zabialla, Peter Kiiru Gathinji, Nuomin, Riyan Baek, Souta Ashida, Ayumi Miyake, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021.11.3  ISAB Organizing Committee

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    Language：English  

    Venue：Ho Chi Minh City and Okayama-shi   Country：Japan  

  • Changes in blood metabolites concentration and fecal microbiota of dairy calves before and after weaning International conference

    Nuomin, Daohu Ao, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021.11.3  ISAB Organizing Committee

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    Language：English   Presentation type：Oral presentation (general)  

    Venue：Ho Chi Minh City and Okayama-shi   Country：Japan  

  • Bacterial and fungal microbiota of guinea grass silage stored at moderate and high ambient temperatures with and without wilting International conference

    Jianjian Hou, Naoki Nishino, Takeshi Tsuruta

    International Symposium on Animal Bioscience 2021  2021.11.3  ISAB Organizing Committee

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    Language：English   Presentation type：Oral presentation (general)  

    Venue：Ho Chi Minh City and Okayama-shi   Country：Japan  

  • Changes in the blood metabolites concentration, milk composition, and milk microbiota of Holstein cows during a lactation period International conference

    Peter Kiiru Gathinji, Karin Akada, Daohu Ao, Sayo Nibuno, Masumi Kanadani, Takeshi Tsuruta, Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021.11.3  ISAB Organizing Committee

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    Language：English   Presentation type：Oral presentation (general)  

    Venue：Ho Chi Minh City and Okayama-shi   Country：Japan  

  • The relationship between milk, udder skin, bedding, and fecal microbiota in a dairy farm International conference

    Daohu Ao, Karin Akada, Peter Kiiru Gathinji, Sayo Nibuno, Masumi Kanadani, Takeshi Tsuruta Naoki Nishino

    International Symposium on Animal Bioscience 2021  2021.11.3  ISAB Organizing Committee

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    Language：English   Presentation type：Oral presentation (general)  

    Venue：Ho Chi Minh City and Okayama-shi   Country：Japan  

  • 次世代シーケンサー（NGS）解析から見えてきた乳汁細菌叢と環境細菌叢の関係 Invited

    西野直樹

    第25回日本乳房炎研究会  2021.10.24  日本乳房炎研究会

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：仙台市   Country：Japan  

  • バンカーサイロで調製したトウモロコシホールクロップサイレージの細菌叢と真菌叢

    Hou Jianjian・西野直樹

    日本畜産学会129回大会  2021.9.15  日本畜産学会

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：仙台市   Country：Japan  

  • A study of the relationship between milk, udder skin, bedding, and feces microbiota in a dairy farm

    Aodaofu, Akada Karin, Gathinji Peter Kiiru, Takeshi Tsuruta, Naoki Nishino

    2nd International Conference on Animal Production for Food Sustainability  2021.6.16 

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  • 貯蔵温度による発酵TMRの細菌叢および真菌叢の変動

    Wali Ajmal・西野直樹

    2021年度日本草地学会新潟大会  2021.3.20  日本草地学会

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：新潟市   Country：Japan  

  • 酢酸型発酵を示したギニアグラスサイレージの細菌叢と真菌叢

    Hou Jianjian・西野直樹

    2021年度日本草地学会新潟大会  2021.3.20  日本草地学会

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：新潟市   Country：Japan  

  • 酢酸型発酵を示したギニアグラスサイレージの細菌叢と真菌叢

    侯健健, 西野直樹

    2021年度日本草地学会新潟大会  2021.3.20 

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  • 貯蔵温度による発酵TMRの細菌叢および真菌叢の変動

    Wali Ajmal, 西野直樹

    2021年度日本草地学会新潟大会  2021.3.20 

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  • 次世代シーケンサー（NGS）解析から見えてきた乳汁細菌叢と 環境細菌叢の関係 Invited

    西野直樹

    第25回 日本乳房炎研究会学術集会  2020.11.13 

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  • An investigation of gut microbiota of Holstein heifers before and after weaning

    Nuomin, Hou Jianjian, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • Gut microbiota differences between Siberian Husky, German Shepherd, Shih Tzu, and Toy Poodle

    Kondo Masako, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • Fermentation and microbiota of guinea grass silage stored at two different temperatures

    Hou Jianjian, Wali Ajmal, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • A pilot study of the microbiota of milk, udder, and feces of dairy cows

    Akada Karin, Wali Ajmal, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • Acceptable storability and aerobic stability of by-products ensiled as a total mixed ration

    Wali Ajmal, Hou Jianjian, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • Assessment of nutritional properties of Sacha inchi (Plukenetia volubilis L.) oil using mice

    Sivixay Souliphone, Gathinji Peter Kiiru, Nuomin, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • Modulatory effects of dietary protein on the composition and fermentation of gut microbiota in rats fed cellulose and raffinose

    Gathinji Peter Kiiru, Sivixay Souliphone, Nuomin, Tsuruta Takeshi, Nishino Naoki

    Symposium on Application of Advanced Technologies in Agriculture  2020.8.7 

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  • 貯蔵温度による発酵TMRの発酵特性、好気的安定性および細菌フローラの変動

    関西畜産学会  2011 

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  • Lactobacillus panisおよびLactobacillus frumentiを添加したイタリアンライグラスおよびトウモロコシサイレージの発酵特性と好気的安定性

    日本草地学会第67回発表会  2011 

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  • 予乾および糖蜜を添加したギニアグラスサイレージの細菌フローラ

    日本草地学会第67回発表会  2011 

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  • イタリアンライグラスおよびギニアグラスから調製したダイレクトカットおよび予乾サイレージの発酵特性と細菌フローラ

    日本草地学会第67回発表会  2011 

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  • Aerobic stability and instability of silages caused by bacteria

    2nd International Symposium on Forage Quality and Conservation  2011 

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  • Effect of inoculation of Acetobacter pasteurianus at ensiling and after silo opening on fermentation products, aerobic stability, and bacterial community of whole crop corn silage.

    2nd International Symposium on Forage Quality and Conservation  2011 

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  • Non-plate methods for silage microorganisms identification

    2nd International Symposium on Forage Quality and Conservation  2011 

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  • 国産粗飼料を利用した発酵TMRの品質調査

    関西畜産学会  2011 

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  • Bacterial community of direct-cut and wilted guinea grass silage stored with and without molasses.

    2nd International Symposium on Forage Quality and Conservation  2011 

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  • トウモロコシサイレージの貯蔵および好気的変敗におけるAcetobacter pasteurianus の役割

    関西畜産学会  2011 

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  • 乳酸菌および糖蜜を添加したアルファルファサイレージの発酵特性、好気的安定性および細菌フローラ

    関西畜産学会  2011 

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  • 無添加あるいは乳酸菌を添加したロールベールサイレージの発酵特性と細菌群集の均一性

    日本畜産学会第112回大会  2010 

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  • トウモロコシサイレージの好気的変敗に対するAcetobacter pasteurianusの添加効果

    日本草地学会第66回発表会  2010 

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  • 発酵TMRの発酵特性と細菌群集の季節変動

    日本畜産学会第112回大会  2010 

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  • 国内で流通する発酵TMRの微生物フローラ－サワードウと共通する多様なヘテロ発酵型乳酸菌の存在－

    日本草地学会第66回発表会  2010 

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  • Lactobacillus plantarumおよびLactobacillus brevisを添加したサイレージの微生物フローラ

    日本草地学会第66回発表会  2010 

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  • イタリアンライグラスサイレージの貯蔵および好気的変敗過程における微生物フローラの変化

    日本草地学会第66回発表会  2010 

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  • コントラクタが調製したトウモロコシサイレージの細菌群集

    日本畜産学会第112回大会  2010 

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  • Storage properties and bacterial communities of wilted guinea grass and rhodes grass silages

    日本畜産学会第110回大会  2009 

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  • イタリアンライグラスおよびトウモロコシサイレージの好気的変敗に対するPediococcus acidilacticiとLactobacillus brevisの抑制効果

    関西畜産学会第59回大会  2009 

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  • バンカーサイロから分離したAcetobacter pasteurianusの性質とトウモロコシサイレージにおける生残性

    関西畜産学会第59回大会  2009 

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  • Aerobic stability of Italian ryegrass and corn silages treated with combined inoculation of Pediococcus acidilactici and Lactobacillus brevis

    15th International Silage Conference  2009 

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  • Variations in bacterial community and survival of inoculated bacteria in bunker silos assessed by denaturing gradient gel electrophoresis profiles

    15th International Silage Conference  2009 

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  • A survey of bacteria community structure of commercial total mixed ration silage

    15th International Silage Conference  2009 

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  • Inoculation effects and survival during ensilage of lactic acid bacteria derived from plant feeds and calf feces

    15th International Silage Conference  2009 

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  • Microflora associated with ensiling of wilted guinea grass as determined by culture-dependent and culture-independent analyses

    15th International Silage Conference  2009 

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  • Assessment of wilted guinea grass silage stored in laboratory-scale and big bale silo

    15th International Silage Conference  2009 

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  • バンカーサイロにおける微生物分布の不均一性－1年以上貯蔵した無添加トウモロコシサイレージにおける調査事例－

    日本草地学会第65回発表会  2009 

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  • Occurrence and survival in whole crop corn silage of Acetobacter pasteurianus

    15th International Silage Conference  2009 

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  • 子牛糞便あるいは発酵飼料から分離した乳酸菌のトウモロコシサイレージにおける生残性

    日本草地学会第65回発表会  2009 

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  • Influence of bacterial inoculants and storage period on fermentation and bacterial community of wilted guinea grass silage

    日本草地学会第65回発表会  2009 

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  • Variations in bacterial communities in bunker silos managed by a contractor

    日本草地学会第65回発表会  2009 

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  • 国内で流通している発酵TMRの実態調査

    日本畜産学会第110回大会  2009 

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  • ビール粕を主体とする発酵TMRの好気的安定性に関わる微生物

    日本畜産学会第109回大会  2008 

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  • 開封後1週間空気にさらした発酵TMRの飼料価値

    日本草地学会第64回発表会  2008 

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  • Microbial composition and fermentation products of guinea grass silage as influenced by wilting and storage period.

    日本草地学会第64回発表会  2008 

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  • 飼料イネホールクロップサイレージのアルコール生成に対するLactobacillus buchneriの抑制効果

    日本草地学会第63回発表会  2007 

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  • 発酵生成物、生菌数およびDGGEバンドパターンからみたパウチサイロとロールベールサイロの類似性

    日本畜産学会第107回大会  2007 

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  • ビール粕あるいはトウフ粕を主体とする発酵TMRの貯蔵特性と微生物群集の解析

    日本畜産学会第107回大会  2007 

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  • 混合素材の違いによるTMRサイレージの発酵特性と好気的安定性の変化

    日本草地学会第61会発表会  2006 

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  • 食品工場から排出される廃糖液の乳酸菌培養基材としての利用

    日本草地学会第62会発表会  2006 

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  • 食品副産物を多用したTMRサイレージの貯蔵性制御－PCR-DGGEによる微生物の群集解析－

    日本畜産学会第106会大会  2006 

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  • Ensiled total mixed ration: A non-conventional silage supporting animal production in Japan.

    2nd China-Japan-Korea Symposium on Grassland Agriculture and Animal Production  2006 

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  • イタリアンライグラスロールベールサイレージにおける微生物叢の不均一性

    日本草地学会第61会発表会  2006 

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  • L. buchneri、L. plantarum（畜草1号）あるいはFJLB（付着乳酸菌事前培養液）を添加したイネホールクロップサイレージの発酵特性

    関西畜産学会第55回大会  2005 

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  • 飼料イネホールクロップサイレージにおける2,3-ブタンジオールの生成頻度

    日本草地学会第60回発表会  2005 

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  • 飼料イネホールクロップサイレージにおけるアルコールの生成とLactobacillus buchneriによる抑制

    日本草地学会第60回発表会  2005 

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  • Lactobacillus buchneriを添加したトウモロコシホールクロップサイレージの乳牛における利用性

    日本畜産学会第104回大会  2005 

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  • 食品副産物を多用したTMRサイレージの貯蔵性制御-アルファルファの有無と空気注入による好気的安定性の変動-

    日本畜産学会第105回大会  2005 

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  • Lactobacillus buchneriを添加したトウモロコシホールクロップ及びTMRサイレージにおけるポリアミン生成量

    日本草地学会第59回発表会  2004 

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  • 飼料イネホールクロップサイレージの発酵特性とLactobacillus buchneriの添加効果

    関西畜産学会第54回大会  2004 

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  • 緑茶殻のサイレージ調製過程におけるカテキン類の消長

    日本畜産学会第103回大会  2004 

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  • 飼料イネホールクロップサイレージにおけるLactobacillus buchneriの添加効果

    日本草地学会第59回発表会  2004 

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  • ビール粕を多用したTMRサイレージの好気的安定性に関する一考察

    関西畜産学会第53回大会  2003 

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  • 1,2-プロパンジオール生成菌Lactobacillus buchneriによる牧草サイレージの好気的変敗抑制

    日本畜産学会第101回大会  2003 

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  • ビール粕を主体とするTMR型サイレージの優れた好気的安定性について

    日本草地学会第58回発表会  2003 

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  • Evaluation of Lactobacillus buchneri derived from by-products ensiling as an inoculum for whole crop maize silage.

    13th International Silage Conference  2002 

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  • ヘテロ発酵型乳酸菌等によるサイレージ発酵と好気的変敗防止―1,2-プロパンジオール生成菌Lactobacillus buchneriの機能―

    日本草地学会大会小集会―サイレージ研究の課題と今後の展望―  2002 

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  • 1,2-プロパンジオール生成菌Lactobacillus buchneriの好気的変敗抑制機能―イタリアンライグラスサイレージの発酵特性と好気的安定性の変化―

    関西畜産学会第52回大会  2002 

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  • 1,2-プロパンジオールを生成するヘテロ型乳酸菌Lactobacillus buchneriを添加したトウモロコシサイレージの発酵特性と好気的安定性

    日本草地学会第57回発表会  2002 

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  • TMR型混合サイレージから分離した1,2-プロパンジオール生成菌Lactobacillus buchneriのサイレージ添加剤としての特性

    日本畜産学会第100回大会  2002 

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  • トウモロコシサイレージ中に生じた1,2-プロパンジオールについて

    日本畜産学会第98回大会  2001 

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  • ビール粕と発泡酒粕の飼料特性の比較―製造会社，製造ロットおよび脱水の有無による化学成分の変動―

    日本草地学会第56回発表会  2001 

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  • サイレージから分離した1,2-プロパンジオール生成菌Lactobacillus buchneriの性質

    関西畜産学会第51回大会  2001 

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  • ビール粕および発泡酒粕サイレージの発酵特性―マルトース添加―

    日本畜産学会第98回大会  2001 

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Awards

  • 研究奨励賞

    1999   日本草地学会  

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    Country：Japan

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Research Projects

  • ジャージー種によるA2牛乳及び乳製品の開発事業

    2021.06 - 2022.03

    日本中央競馬会  令和３年度日本中央競馬会畜産振興事業 

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\3920000 （ Direct expense: \3300000 、 Indirect expense：\620000 ）

  • New strategies for controlling silage microbiota using integrated analysis of bacteriome and mycobiome

    Grant number：19H03106  2019.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    西野 直樹, 鶴田 剛司

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    Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

    実験室規模で発酵TMRおよびトウモロコシサイレージを調製した。10℃、25℃、室温（20-35℃）および40℃で2種類の発酵TMRを貯蔵し、2週間および2ヶ月後の発酵生成物、細菌叢および真菌叢を調べた。
    10℃で貯蔵すると、2週間では発酵が微弱だったが、2ヶ月になると十分量（>50 g/kg DM）の乳酸が生成した。25℃、室温および40℃で貯蔵すると、貯蔵初期から発酵が活発化したが、25℃では酢酸よりもエタノールが多いのに対し、室温および40℃では酢酸がエタノールより多いという特徴があった。乳酸生成量の違いは細菌叢とくにLactobacillusとAcinetobacterの割合で説明できたが、酢酸とエタノールのどちらが優勢となるかを説明するには真菌叢を情報が有用で、Kazachstaniaが多ければエタノールが、DiutinaとKluyveromycesが多ければ酢酸が優勢であった。
    トウモロコシサイレージは、発酵TMRから分離した乳酸菌L. buchneri、L. formosensis、L. parafarrgaginis、L. hammesii、L. paralimentariusおよびL. farciminis を添加して調製した。発酵TMRは開封後に好気的変敗を起こしにくいという特徴があるが、L. buchneriを除き変敗防止能は確認されていない。L. buchneriに匹敵したのはL. parafarrgaginisとL. farciminisであった。細菌叢解析は作用機序の違いを明らかにし、L. buchneriはEnterobacterをL. parafarrgaginisはAcetobacterを強く抑制していた。防止能がなかったL. hammesi以外はBacillusを抑制しており、多様な乳酸菌が相加、相乗的に変敗防止に作用していると考えられた。

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  • Investigation into lactic acid bacteria involving in resistance to aerobic stability of total mixed ration silage

    Grant number：24580390  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NISHINO Naoki

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    Grant amount：\5590000 （ Direct expense: \4300000 、 Indirect expense：\1290000 ）

    Evidence has shown that total mixed ration (TMR) silage can resist deterioration after silo opening. From culture-independent microbiota analysis of practical TMR silage, several difficult-to-culture lactic acid bacteria, such as Lactobacillus pontis, Lactobacillus fructivorans, were suggested to be involved. We successfully isolated L. fructivorans using liver infusion sake medium, and then confirmed its inhibitory activity against aerobic spoilage.

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  • Evaluation of Acetobacter spp. as a novel inoculant to improve silage management

    Grant number：21380166  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    NISHINO Naoki

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    Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

    Acetobacter pasteurianus was isolated from bunker-made untreated corn silage as the predominant bacterium. The presence of A. pasteurianus was confirmed by denaturing gradient gel electrophoresis, and in other bunker-made corn silages produced in different regions including Beijing, China. Using the isolate, the effects of A. pasteurianus on fermentation, aerobic stability and bacterial community were determined. Addition of A. pasteurianus before ensiling improved aerobic stability in corn but not in grass and rice silage, whereas addition of A. pasteurianus after silo opening accelerated aerobic spoilage in corn silage. These results indicate that A. pasteurianus can inhabit bunker-made corn silage, and may show significant interactions with other bacterial species associated with anaerobic storage and aerobic deterioration.

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  • Bacterial community analyses for the selection of homo-fermentative lactic acid bacteria with inhibitory activity against aerobic silage deterioration

    Grant number：19580311  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NISHINO Naoki

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    Grant amount：\4680000 （ Direct expense: \3600000 、 Indirect expense：\1080000 ）

    好気的変敗(発酵飼料が開封後に腐敗すること)が起こりにくいTMRサイレージ(発酵混合飼料)の細菌群集を解析して、変敗防止能をもつホモ発酵型乳酸菌の探索と作用幾序の解明を試みた。培養法およびPCR-DGGEによる調査結果から、L. delbrueckii、P. acidilactici、S. bovis、L. paracasei、L. plantarumを候補細菌としてリストアップし、牧草への添加試験によってP.acidilacticiに変敗抑制効果があることを確認した。

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  • PCR-DGGE analysis of silage microorganisms and its application to food quality control

    Grant number：17580236  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NAOKI Nishino

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    Grant amount：\3600000 （ Direct expense: \3600000 ）

    It has become a common practice in Japan that high-moisture by-products are stored with dry feeds as an total mixed ration silage. This may minimize the risk of effluent production and can omit the time for mixing prior to feeding. Unpalatable by-products could be incorporated into a TMR silage, if their odors and flavors are altered through the fermentation in a silo. An interesting property of the TMR silage is high stability on exposure to air. Heating would not occur even in summer, whereas non-ensiled TMR mixture may deteriorate quite rapidly. Conventional criteria do not account well for the stability, because the TMR silage can remain unheated even with a large number of yeasts and a substantial level of substrates (sugars and lactic acid) for the growth of aerobic microorganisms. We have investigated the factors involved in resistance to deterioration by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and some interesting results are obtained as follows.
    1. When wet brewers grains and soybean-curd refuse were stored alone or in combination with other feeds as a TMR silage, deterioration did not occur only in TMR silages. Without anaerobic fermentation, TMR mixture also spoiled quite easily.
    2. Lactobacillus buchneri, a hetero-fermentative lactic acid bacteria which is capable of enhancing aerobic stability, was not found in the silage material, but appeared from the early ensiling period in TMR silage containing wet brewers grains. Although aerobic stability was similarly high in TMR silage containing soybean-curd refuse, L. buchneri was not detected regardless of ensiling period.
    3. Weisella sp. and Lactobacillus brevis was found in both the stable TMR silages. Unstable by-products silage stored alone did not contain these two lactic acid bacteria.
    4. We could isolate those L. buchneri, L. brevis and Weisella confusa from the following experiment, hence now are planning to inoculation experiment to determine if these bacterial mixture can actually improve the aerobic stability of silage.

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  • Influence and function of a 1,2-propanediol-producing lactic acid bacterium, Lactobacillus buchneri, in inhibiting aerobic deterioration of silage

    Grant number：15580234  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NISHINO N.

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    Grant amount：\3700000 （ Direct expense: \3700000 ）

    A 1,2-propanediol-producing microorganism, Lactobacillus buchneri, was used as a novel silage starter. The activity to inhibit aerobic deterioration and its related factors were studied. Materials inoculated were whole crop corn, Italian ryegrass, festulolium, whole crop rice and total mixed ration ; changes in microbial flora, fermentation products and aerobic stability were determined. Biogenic amines were also determined in silage to ascertain the safety of L.buchneri in feed preservation. The nutritive value of inoculated silage was evaluated with dairy cows and dry matter intake, milk yield, milk composition and rumen fermentation were determined. The results are as follows.
    1.Inoculation of L..buchneri decreased the number of yeasts and improved the silage stability after exposure to air. This benefit was accompanied with reduction of lactic acid and increase of acetic acid and 1,2-propanediol. Wilting diminished the effects of L.buchneri on fermentatio products, while the activity of inhibiting deterioration was kept comparable to that in direct-cut silage. Ensiled total mixed ration did not heat after unloading regardless of L.buchneri inoculation.
    2.The three L.buchneri strains of our collection did not produce biogenic amines in a screening media. These characteristics were confirmed in corn, festulolium and TMR silage, thereby non-amine-producing L.buchneri could be considered as a safe silage starter.
    3.Benefits of L.buchneri were also found when ensiled in a practical scale silo, but the extents were rather small compared with those in laboratory scale silos. Silages were fed to dairy cows as an ingredient of total mixed ration and no difference was found in dry matter intake, milk yield, milk composition and rumen fluid characteristics. Use of L.buchneri can improve aerobic stability without a decrease in animal performance.
    4.Addition of L.buchneri greatly decreased the production of ethanol and 2,3-butanediol in whole crop rice silage. Even after 12 months of ensilage, the L.buchnei added could remain in silage as a dominant microorganism.

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  • Evaluation of nutritional significance and microbial production of 1, 2-propanediol in silage

    Grant number：13660267  2001 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NISHINO Naoki

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    Grant amount：\4100000 （ Direct expense: \4100000 ）

    This research was aimed to identify microbial factors responsible for 1, 2-propanediol formation in silage. The value of dietary 1, 2-propanediol in ruminants was also investigated.
    From a mixed silage primarily containing wet brewers grains, a 1, 2-propanediol producing microorganism was isolated. This gram-positive, catalase-negative and heterofermentative lactic acid bacteria was identified as Lactobacillus buchneri. When L. buchneri was inoculated to whole-crop maize and grasses, significant amounts of 1, 2-propanediol were produced in the silages. The production was coupled with a decrease in lactate and an increase in acetate, and was enhanced when storage period was prolonged The highest content was 77 g/kg DM in this study.
    Aerobic stability of silages was considerably improved when L. buchneri was inoculated, probably because the increased acetate could act as an antimicrobial component and lower yeast population during ensilage.
    Exogenous 1, 2-propanediol was degraded by rumen microorganisms and the ratio of acetate to propionate decreased, while not affecting the intake and preference of total mixed ration. Also, the intake of total mixed ration was not altered, when L. buchneri inoculated silage was incorporated.
    It is concluded that L. buchneri can be a major 1, 2-propanediol producing microorganism in silage and could be used as a novel additive to inhibit aerobic deterioration. The intake and preference of diets would be unaffected by 1, 2-propanediol, when inoculated silages were offered as ingredients of total mixed ration.

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  • 乾草およびサイレージ利用システムにおける牧草細胞壁のダイナミック解析

    Grant number：10760157  1998 - 1999

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    西野 直樹

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    Grant amount：\1900000 （ Direct expense: \1900000 ）

    寒地型イネ科牧草(イタリアンライグラス),寒地型マメ科牧草(アルファルファ)および暖地型イネ科牧草(ローズグラスおよびギニアグラス)を用い,細胞壁分解酵素の添加がサイレージの発酵特性ならびにルーメン内消化動態に及ぼす影響について検討した.各牧草を予乾した後,酵素を50mg/kgの割合で添加してサイレージを調製した.45日間嫌気的に貯蔵した後,凍結乾燥試料をヤギのルーメン内でin situ培養して細胞壁成分の消化動態を測定した.得られた結果は以下のとおりである.
    サイレージの貯蔵性は細胞壁分解酵素の使用によって向上したが,その程度は牧草種によって異なり,寒地型イネ科牧草に比べマメ科牧草および暖地型牧草で改善効果が大きかった.暖地型牧草サイレージでは,酸素を添加しなくても貯蔵日数の延長にともなって細胞壁成分が減少する傾向が認められ,サイレージ発酵の基質として細胞壁成分が利用されていることが示唆された.ルーメン内における細胞壁成分の消化速度はマメ科牧草サイレージが大きな値を示したが,酵素を添加するとその値は有意に低下した.この酵素添加にともなう消化速度の低下はイネ科牧草サイレージでは認められず,細胞壁成分の潜在的な消化・分解性はイネ料牧草とマメ科牧草では大きく異なることが示された.暖地型牧草サイレージでは,貯蔵中にその一部が分解されたにも係わらず消化速度が低下しなかったことから,乾草よりもサイレージ化する方が飼料全体の利用性を高めるうえで有利と考えられた.

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  • 飼料の保存性および安全性におけるメイラード反応生成物の役割

    Grant number：08760249  1996

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    西野 直樹

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    Grant amount：\1100000 （ Direct expense: \1100000 ）

    1.牧草類の貯蔵性に対するメイラード反応生成物の意義
    D-グルコースあるいはD-キシロースとグリシンあるいはL-リジンの混合溶液を95℃で24時間加熱してメラノイジンを調製し,透析後凍結乾燥して得た粉末をアルファルファに0.5%(新鮮物当たり)添加して60日間嫌気的に貯蔵した.得られた結果は以下のとおりである.
    メラノイジンの添加は乳酸菌数を増加させる傾向を示したが,pHの低下およびタンパク質分解の抑制効果は認められなかった.キシロースとグリシンから調製したメラノイジンを添加すると,乳酸菌の増殖が抑制されて乳酸生成量が減少した.これらのことから,メイラード反応生成物は牧草類の貯蔵性に対して負の要因となりうるが,その効果は反応に係わる基質によって異なることが示された.
    2.鳥類の脂質代謝および卵黄脂質濃度に対するメイラード反応生成物の影響
    D-グルコースとL-リジンから調製したメラノイジンを0,1,2および4%添加して飼料を調製し,25週令の産卵ウズラに給与して飼養試験を行った.得られた結果は以下のとおりである.
    試験期間中の飼料摂取量および産卵率はメラノイジン添加の影響を受けなかった.血漿のコレステロール濃度は2%添加区で有意に低下したが,4%添加区の値は無添加区のそれとほぼ同様であった.肝臓脂質,卵黄コレステロールおよび卵黄脂肪酸(C16:0,C18:0,C18:1およびC18:2)濃度は飼料間に差が認められなかった.これらのことから,メイラード反応生成物の摂取により鳥類の血漿コレステロール濃度は変動しうるが,それらは必ずしも卵黄脂質濃度に反映しないことが示された.

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  • 反芻動物におけるメラノイジンの栄養学的意義

    Grant number：07760251  1995

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    西野 直樹

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    Grant amount：\1100000 （ Direct expense: \1100000 ）

    1.メラノイジンのルーメン内分解性を知ることを目的とし、単糖とアミノ酸から非透析性の高分子メラノイジンを調製してin vitroにおけるルーメン内培養実験に供した。反応基質の糖にはグルコースとキシロースを、アミノ酸にはグリシンとリジンを用い、得られた4種類のメラノイジンの培養前後のゲル濾過溶出パターンからその分解性を推定した。
    (1)メラノイジンの生成量はグルコースよりもキシロースを用いた方が多く、かつグリシンよりもリジンを用いた方が多かった。また分子量や溶解度もメラノイジンの種類によって異なることが示された。
    (2)培養前後のゲル濾過溶出パターンはいずれのメラノイジンを用いた場合も変化がなく、メラノイジンはルーメン内で分解されないと推察された。
    2.反芻動物の蛋白質利用性に対するメラノイジンの作用を知ることを目的とし、高あるいは低分子に分画したメラノイジンがルーメン内および小腸内の蛋白質消化に及ぼす影響をin vitroで検討した。メラノイジンはグルコースとグリシンあるいはグルコースとリジンから調製し、分画後ルーメン内培養実験および透析セル内でのペプシン・パンクレアチン消化実験に供した。
    (1)培養したルーメン液中のペプチドおよびアンモニア態N濃度は、いずれのメラノイジンを添加しても有意な変化を示さず、メラノイジンはルーメン内での蛋白質消化には大きく影響しないことが示された。
    (2)パンクレアチン消化によって透析膜外に溶出したペプチドの濃度は、グルコースとリジンから調製した高分子メラノイジンを添加すると低くなり、逆にグルコースとグリシンから調製した低分子メラノイジンを添加すると高くなった。すなわち、メラノイジンは基質および分子量によって蛋白質の小腸内消化に促進あるいは抑制因子として働くことが示唆された。

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  • ルーメン内分解性制御の観点からみた蛋白質構成アミノ酸のラセミ化の意義

    Grant number：06760232  1994

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    西野 直樹

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    Grant amount：\1000000 （ Direct expense: \1000000 ）

    1.アルカリ処理蛋白質の栄養学的意義を知る目的から、大豆粕に乾物当り0、2.5、5及び10%の水酸化ナトリウム(NaOH)を噴霧した後、80℃で加熱乾燥して消化試験に供した。消化試験はルーメンカニューレを装着した山羊4頭を用いて行い、成分消化率、窒素出納、ルーメン内発酵及び蛋白質のルーメン内分解特性について検討した。以下に示す結果は現在印刷中である。
    (1)NaOH処理量が高くなるに従いリジン、トレオニン及びアルギニン量が減少し、リジノアラニン生成量が増加した。しかし、これらの大豆粕を10%含む混合飼料を山羊に給与すると、消化率は変化しなかったが窒素出納は改善される傾向を示した。
    (2)NaOH2.5及び5%処理は蛋白質のルーメン内分解性を低下させたが、10%処理は逆に蛋白質の溶解性を高めた。また、これらの結果はルーメン内アンモニア濃度の変化からも裏付けられた。
    (3)リジノアラニン生成量と蛋白質のルーメン内分解速度の間に有意な負の相関関係が認められ、飼料蛋白質のルーメン内分解性低下に、アミノ酸の架橋形成が関与していることが示された。
    2.アルカリ及び加熱処理によるルーメン内蛋白質分解の制御を目的として、大豆粕に乾物当り0、1、2、4及び8%のNaOHを噴霧した後、60及び100℃で加熱乾燥してアミノ酸分析及びin situバッグ法による蛋白質分解率の測定に供した。分解率の結果を数学モデルに当てはめて、ルーメン内における蛋白質の消化動態を測定した。現在に以下の結果を明かにし、分析を継続中である。
    (1)リジノアラニンの生成量は、加熱温度及びNaOH処理量が高くなるに従って増加し、その生成量は各処理に対して用量依存的であった。
    (2)蛋白質の分解速度は各処理によって直線的に低下し、リジノアラニン生成に伴う分解速度低下の機構が明確となった。
    (3)蛋白質の有効分解率の変化から、最適処理条件は4%のNaOH処理と100℃の加熱乾燥の組み合わせであると判断された。
    蛋白質構成アミノ酸のラセミ化(D-アミノ酸の生成量)については、ガスクロマトグラフィーによる分析条件を検討中であり、実験1及び2の試料について定量した後、報告する予定である。

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  • 飼料蛋白質のルーメン内分解性と架橋形成アミノ酸の関係

    Grant number：05760203  1993

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    西野 直樹

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    Grant amount：\900000 （ Direct expense: \900000 ）

    1.開花期のアルファルファ1番草を材料とし、これに乾物当り0、1.16、2.32及び4.64%のNaOHを噴霧した後、凍結乾燥あるいは加熱乾燥(60℃及び120℃)として試料を調製した。各試料についてアミノ酸分析を行うとともに、in situバッグ法によりルーメン内蛋白質分解率を測定した。得られた結果を数学モデルに当てはめて、蛋白質のルーメン内における消化動態を推定した。以下に示す結果をまとめ、投稿し、受理されている。
    (1)アミノ酸の架橋形成を示すリジノアラニンの生成は、加熱温度及びNaOH処理量が高くなるに従い増加した。その生成量は各処理に対して用量依存的であったが、NaOH処理よりも加熱処理の方が影響が大きかった。
    (2)蛋白質のルーメン内可溶性区分の割合は加熱及びNaOH処理いずれによっても低下し、相対的に分解性区分の割合は減少した。分解速度も各処理によって低下したが、その効果は、NaOH処理よりも加熱処理の方が大きかった。
    (3)リジノアラニン生成量と蛋白質のルーメン内分解速度の間に有意な負の相関関係が認められ、飼料蛋白質のルーメン内分解性低下に、アミノ酸の架橋形成が関与することが示された。
    2.市販の大豆粕を用い、これに乾物当り0、2.5及び10%のNaOHを噴霧した後、80℃で乾燥してアミノ酸分析及び消化試験に供した。消化試験はルーメンカニューレを装着した山羊4頭を用いて行い、これまでに以下に示す結果を得ている。
    (1)NaOH処理量が高くなるに従いリジノアラニン生成量は増加し、逆にリジン、トレオニン及びアルギニン量は減少した。しかし、これらを給与した山羊の窒素出納は改善される傾向を示した。
    (2)NaOH2.5及び5%処理は蛋白質のルーメン内分解性を低下させたが、10%処理は逆に蛋白質の溶解性を高めることが示され、NaOHの適性処理量は乾物当り5%以下と判断された。
    なおこれらリジノアラニンを含むアルカリ処理蛋白質の給与が、ルーメン内ペプチド代謝及び微生物態蛋白質合成に及ぼす影響については、引き続き検討していく予定である。

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  • Seminar in Animal Nutrition and Feed Science （2021academic year） Prophase  - その他

  • Topics in Animal Nutrition （2021academic year） Late  - 火1,火2

  • Functional Feed and Food Science （2021academic year） Late  - その他

  • Animal Production and Management （2021academic year） 1st and 2nd semester  - 月1,月2

  • Undergraduate's-level thesis research （2021academic year） 1st-4th semester  - その他

  • Undergraduate's-level thesis research （2021academic year） 1st-4th semester  - その他

  • Introduction to Animal Science 2 （2021academic year） Fourth semester  - 水5,水6

  • Introduction to Animal Science 2 （2021academic year） Fourth semester  - 水5～6

  • Specific Research of Science for Bio-Production （2021academic year） Year-round  - その他

  • Specific Research of Science for Bio-Production （2021academic year） Year-round  - その他

  • Seminars in Special Field of Study 1 （2021academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2021academic year） 3rd and 4th semester  - その他

  • Seminars in Special Field of Study 2 （2021academic year） 1st and 2nd semester  - その他

  • Introduction to Agrobiology 1 （2021academic year） 1st semester  - 水5,水6

  • Introduction to Agrobiology 1 （2021academic year） 1st semester  - 水5～6

  • Advances in Animal Science （2020academic year） Late  - 金7,金8

  • Introduction to Animal Science （2020academic year） Second semester  - 火5,火6

  • Practice in an animal husbandry station （2020academic year） Summer concentration  - その他

  • Introduction to Innovation （2020academic year） Late  - 月7,月8,金7,金8

  • Introduction to Innovation （2020academic year） Late  - 月7～8,金7～8

  • Course Seminar 1 （2020academic year） 1st and 2nd semester  - 水3

  • Course Seminar 2 （2020academic year） 3rd and 4th semester  - 水3

  • Course Seminar 4 （2020academic year） 3rd and 4th semester  - その他

  • Seminar in Animal Nutrition and Feed Science （2020academic year） Prophase  - その他

  • Seminar in Animal Nutrition and Feed Science （2020academic year） Late  - その他

  • Seminar in Animal Nutrition and Feed Science （2020academic year） Late  - その他

  • Seminar in Animal Nutrition and Feed Science （2020academic year） Late  - その他

  • Seminar in Animal Nutrition and Feed Science （2020academic year） Prophase  - その他

  • Seminar in Animal Nutrition and Feed Science （2020academic year） Prophase  - その他

  • Topics in Animal Nutrition （2020academic year） Late  - 火1,火2

  • Animal Nutrition 1 （2020academic year） Third semester  - 水1,水2

  • Animal Nutrition 2 （2020academic year） Fourth semester  - 水1,水2

  • Functional Feed and Food Science （2020academic year） Late  - その他

  • Animal Production and Management 1 （2020academic year） 1st semester  - 月1,月2

  • Animal Production and Management 2 （2020academic year） Second semester  - 月1,月2

  • Undergraduate's-level thesis research （2020academic year） 1st-4th semester  - その他

  • Undergraduate's-level thesis research （2020academic year） 1st-4th semester  - その他

  • Introduction to Animal Science 1 （2020academic year） Third semester  - 金5,金6

  • Introduction to Animal Science 1 （2020academic year） Third semester  - 金5,金6

  • Introduction to Animal Science 2 （2020academic year） Fourth semester  - 金5,金6

  • Introduction to Animal Science 2 （2020academic year） Fourth semester  - 金5,金6

  • Specific Research of Science for Bio-Production （2020academic year） Year-round  - その他

  • Specific Research of Science for Bio-Production （2020academic year） Year-round  - その他

  • Topics in Science for Bio-Production 2 （2020academic year） Summer concentration  - その他

  • Seminars in Special Field of Study 1 （2020academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2020academic year） 3rd and 4th semester  - その他

  • Seminars in Special Field of Study 2 （2020academic year） 1st and 2nd semester  - その他

  • Introduction to Agrobiology 1 （2020academic year） 1st semester  - 水3,水4

▼display all

 

Academic Activities

  • Chair of International Symposium on Animal Bioscience 2021 International contribution

    Role(s)：Planning, management, etc.

    ISAB Organizing Committee  2021.11.3

    　More details

    Type：Competition, symposium, etc. 

  • Editor-in-Chief of Grassland Science International contribution

    Role(s)：Planning, management, etc.,　Review, evaluation

    Grassland Science  2020.4.1

    　More details

    Type：Academic society, research group, etc.