Updated on 2022/11/28

写真a

 
YAMAJI Kozo
 
Organization
Okayama University Hospital Lecturer
Position
Lecturer
Contact information
メールアドレス
External link

Degree

  • 博士(歯学) ( 北海道大学 )

Research Interests

  • Low Intensity Pulsed Ultrasound

  • Aging

  • Dentin formation

  • Growth factors

  • Bone formation

Research Areas

  • Life Science / Conservative dentistry

Education

  • 北海道大学大学院   歯学研究科   博士課程

    1996 - 2000

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    Country: Japan

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  • Hokkaido University   歯学部   歯学科

    - 1996

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    Country: Japan

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  • Hokkaido University    

    - 1996

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Research History

  • University of California San Francisco, Visiting Professor

    2011

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  • Okayama University   Okayama University Hospital

    2007

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  • Senior Assistant Professor, University Hospital of Medicine and Dentistry, Okayama University

    2007

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  • Okayama University   Okayama University Hospital

    2004 - 2007

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  • Hokkaido University   Hokkaido University Hospital

    2000 - 2004

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  • Graduate Student,Hokkaido Univiersity Graduate School of Dental Medicine

    1996 - 2000

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Professional Memberships

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Committee Memberships

  • 象牙質歯髄治療学会   評議員  

       

  • 日本再生歯科医学会   評議員  

       

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    Committee type:Academic society

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  • 日本歯科保存学会   評議員  

       

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    Committee type:Academic society

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Papers

  • Effect of gelatin hydrogel containing BMP-2 on bone formation in aged rats Reviewed

    Yamaji Kozo, Yokoyama Akihito, Matsuzaki Kumoko, Ohara Naoko, Sugaya Tsutomu, Yoshiyama Masahiro

    J Oral Tissue Engin.   19 ( 1 )   10 - 14   2021.9

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  • Assessment of Demineralization Inhibition Effects of Dentin Desensitizers Using Swept-Source Optical Coherence Tomography. Reviewed International journal

    Kumiko Matsuzaki, Yasushi Shimada, Yasuo Shinno, Serina Ono, Kozo Yamaji, Naoko Ohara, Alireza Sadr, Yasunori Sumi, Junji Tagami, Masahiro Yoshiyama

    Materials (Basel, Switzerland)   14 ( 8 )   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    The purpose of this study was to evaluate the mechanism of action and the inhibiting effects of two types of desensitizers against dentin demineralization using pre-demineralized hypersensitivity tooth model in vitro. In this study, we confirmed that a hypersensitivity tooth model from our preliminary experiment could be prepared by immersing dentin discs in an acetic acid-based solution with pH 5.0 for three days. Dentin discs with three days of demineralization were prepared and applied by one of the desensitizers containing calcium fluoro-alumino-silicate glass (Nanoseal, NS) or fluoro-zinc-silicate glass (Caredyne Shield, CS), followed by an additional three days of demineralization. Dentin discs for three days of demineralization (de3) and six days of demineralization (de6) without the desensitizers were also prepared. The dentin discs after the experimental protocol were scanned using swept-source optical coherence tomography (SS-OCT) to image the cross-sectional (2D) view of the samples and evaluate the SS-OCT signal. The signal intensity profiles of SS-OCT from the region of interest of 300, 500, and 700 µm in depth were obtained to calculate the integrated signal intensity and signal attenuation coefficient. The morphological differences and remaining chemical elements of the dentin discs were also analyzed using scanning electron microscopy and energy-dispersive X-ray spectroscopy. SS-OCT images of CS and NS groups showed no obvious differences between the groups. However, SS-OCT signal profiles for both the CS and NS groups showed smaller attenuation coefficients and larger integrated signal intensities than those of the de6 group. Reactional deposits of the desensitizers even after the additional three days of demineralization were observed on the dentin surface in NS group, whereas remnants containing Zn were detected within the dentinal tubules in CS group. Consequently, both CS and NS groups showed inhibition effects against the additional three days of demineralization in this study. Our findings demonstrate that SS-OCT signal analysis can be used to monitor the dentin demineralization and inhibition effects of desensitizers against dentin demineralization in vitro.

    DOI: 10.3390/ma14081876

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  • Effects of Direct Pulp Capping on Hard Tissue Formation by Using Alginate Gel Containing Bone Morphogenetic Protein-2 Reviewed

    Yokoyama Akihito, Yamaji Kozo, Ohara Naoko, Matsuzaki Kumiko, Shimada Yasushi, Yoshiyama Masahiro

    J Oral Tissue Engin,   17 ( 2 )   53 - 58   2019.12

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  • Effects of Transforming growth factor-β1 and Bone morphogenetic protein-2 on bone formation using a bioabsorbable scaffold at palatal sites in rats Reviewed

    Yamaji K, Yokoyama A, Shimada Y, Sugaya T, Ohara N, Matsuzaki K, Nishiyama E, Yoshiyama M

    J Oral Tissue Engin,   16 ( 2 )   74 - 79   2018.12

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  • Effects of aging on bone formation induced by BMP-2 using low-intensity pulsed ultrasound Reviewed

    Yamaji K, Yokoyama A, Matsuzaki K, Ohara N, Nishiyama E, Sugaya T, Yoshiyama M

    Journal of Oral Tissue Engineering   14 ( 1 )   65 - 69   2016

  • Effects of BMP-2 Injected into the Palatal Sites on Bone Formation Reviewed

    YAMAJI Kozo, TAKAHASI Kei, NISHITANI Yoshihiro, IZAWA Shunji, HORIKAWA Gen, NISHIMURA Maiko, HOSHIKA Tomohiro, YOSHIYAMA Masahiro

    Journal of oral tissue engineering   6 ( 1 )   62 - 66   2008

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    Language:English   Publisher:Japanese Association of Regenerative Dentistry  

    This study was designed to evaluate the effects of recombinant human BMP-2 (rhBMP-2) injected into the palatal periosteal sites in Wister rats on bone formation. The implant sites were separately divided into four groups according to the dosage of rhBMP-2 : 0 μg, 0.1 μg, 0.5 μg and 1.0 μg. The rats were sacrificed at 3 weeks after implantation, followed by histopathologic observation and histometric evaluation of new bone. Implants had disappeared at every group and new bone formation was observed at 0.1 μg, 0.5 μg and 1.0 μg. New bone was continuous with the original bone at 0.5 μg and 1.0 μg. Thickness of new bone increased as the dosage increased from 0 μg to 0.5 μg (P<0.05), and did not significantly change as the dosage increased from 0.5 μg to 1.0 μg. These results suggested that rhBMP-2 injected into the palatal periosteal sites could lead to induce new bone formation, although its effects varied according to the dosage of rhBMP-2.

    DOI: 10.11223/jarde.6.62

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  • Dentin Regeneration by Direct Pulp Capping using rhBMP-2 Reviewed

    YAMAJI Kozo, HORIKAWA Gen, NISHITANI Yoshihiro, IZAWA Shunji, YOSHIYAMA Masahiro

    Journal of Oral Tissue Engineering   5 ( 3 )   145 - 149   2008

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    Language:English   Publisher:Japanese Association of Regenerative Dentistry  

    The purpose of this study was to evaluate the effect of direct pulp capping using recombinant human BMP-2 (rhBMP-2) on dentin regeneration. RhBMP-2 (1.0 mg/ml) was dissolved in alginate-based gel (AG). Cavities with the exposed pulp area were prepared of first molars in Wister rats. All cavities were divided into two groups: The exposed pulps were covered with AG contained rhBMP-2 (BMP group), and covered with AG alone (AG group). Then they were sealed with glass ionomer cement (Fuji IILC EM). The animals were sacrificed at 3 and 8 weeks after operation. In both groups, the covered materials were observed at 3 weeks after operation, while they had disappeared and the tertiary dentin formation was observed into the prepared cavity at 8 weeks after operation. Thickness of the tertiary dentin of BMP group was significantly higher than that of AG group (P<0.05). These results suggest that AG is effective scaffold and direct pulp capping using AG contains rhBMP-2 is capable of enhancing dentin regeneration.

    DOI: 10.11223/jarde.5.145

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  • Effects of dose of recombinant human BMP-2 on bone formation at palatal sites in young and old rats. Reviewed

    Kozo Yamaji, Masamitsu Kawanami, Atsushi Matsumoto, Tomoomi Odajima, Yoshihiro Nishitani, Kensuke Iwasaka, Keiko Yoshimitsu, Masahiro Yoshiyama

    Dental materials journal   26 ( 4 )   481 - 6   2007.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC DENTAL MATERIALS DEVICES  

    This study was designed to examine the effects of dose of recombinant human BMP-2 (rhBMP-2) on bone formation at palatal sites in 10-week-old (10w) and 70-week-old (70w) rats, when combined with a polylactate-polyglycolate copolymer/gelatine sponge (PGS). New bone formation was observed at six weeks after implantation. In the 10w rats, thickness of new bone (TNB) increased as the dosage increased from 0 microg to 4 microg, and decreased significantly as the dosage increased from 8 microg to 24 microg. In contrast, in the 70w rats, TNB increased as the dosage increased from 0 microg to 16 microg, and did not significantly change as the dosage increased from 16 microg to 24 microg. These results suggest that the most effective dosage of rhBMP-2 for induction of bone formation varies according to age.

    DOI: 10.4012/dmj.26.481

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  • Influence of Osteoporosis on Bone Formation by Bone Morphogenetic Protein-2 Reviewed

    TSUCHIYA Naoko, ODAJIMA Tomoomi, YAMAJI Kozo, KAWANAMI Masamitsu

    The Japanese Journal of Conservative Dentistry   50 ( 6 )   792 - 798   2007

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    Recombinant human bone morphogenetic protein (BMP)-2 is known to induce significant regeneration of bone, but the influence of osteoporosis on bone formation is not understood. The aim of this study was to investigate the influence of osteoporosis on bone formation by histopathological and histometrical evaluations. Five-week-old KO OCIF/Jcl (homo) mice (osteoporosis group) and C57BL/6NJcl mice (normal group) were used. Atelocollagen gel with 0, 1, 5, 10 and 15 μg/ml BMP-2 were prepared and injected into the periosteum of the mice's cranial bone. Histopathological observation and histometric measurement of new bone and bone density were performed. The Mann Whitney U test was used for statistical significance. Both groups increased new bone in a concentration-dependent manner. At BMP concentrations of 0 and 1μg/ml, neither group showed new bone, and at BMP of 5 and 15μg/ml, the osteoporosis group showed significantly more bone than the normal group (p<0.05). Bone density was significantly lower in the osteoporosis group than in the normal group at BMP 5,10 and 15μg/ml separately (p<0.05). As a result, it was concluded that the amount of new bone induced by BMP-2 increases and the bone density decreases in osteoporosis.

    DOI: 10.11471/shikahozon.50.792

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  • Practice Training of Conservative Dentistry on Postgraduate Clinical Training Course at Okayama University Hospital of Medicine and Dentistry Reviewed

    KONO Takayuki, YAMAJI Kozo, YOSHIYAMA Masahiro, ARAI Hideo, TAKASHIBA Shogo, TORII Yasuhiro

    The Japanese Journal of Conservative Dentistry   50 ( 6 )   731 - 739   2007

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    We have developed and applied a new electronic portfolio system to educate 65 dental residents for postgraduate clinical training in Okayama University Hospital of Medicine and Dentistry since the 2006 academic year. The purpose of this study was to examine the situation of postgraduate clinical training concerned with conservative dental treatment by using our electronic portfolio system in order to improve the postgraduate clinical training program at our hospital. At first, the number of patients whom the dental residents had treated was examined. Next, the records related to conservative dental treatment that the residents had put in the electronic portfolio system were exported and divided into three groups: restorative treatment, endodontic treatment and periodontal treatment, and the details were analyzed. The average number of patients for the period of their internship was 17.0±4.9 persons and the total number of records in the electronic portfolio system was 23,911. The records concerned with conservative dentistry accounted for 47.3% (11,316 records) of the total. The frequency of the restorative, endodontic, and periodontal treatments were 9.4% (2,239 records), 9.8% (2,335 records), and 28.2% (6,742 records), respectively. Detailed analysis demonstrated that residents experienced over 10 cases of "root canal filling" and "scaling and root planing", and over 20 cases of "resin filling", "root canal enlargement and/or application", "periodontal tissue examination", "tooth brushing instruction", "sonic scaling", and "supportive periodontal therapy". The results demonstrated that the dental residents experienced conservative dental treatment with a frequency of over 50% (almost 50% of all dental treatments) during their postgraduate clinical training program in Okayama University Hospital of Medicine and Dentistry, and that they experienced over 10 cases of basic treatment procedures of conservative dentistry.

    DOI: 10.11471/shikahozon.50.731

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  • Influence of the Dose of Recombinant Human BMP-2 on Bone Formation at Palatal Sites in Old Rats Reviewed

    YAMAJI Kozo, ODAJIMA Tomoomi, MATSUMOTO Atsushi, KAWANAMI Masamitsu

    45 ( 4 )   710 - 715   2002.8

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  • Influence of Menatetrenone (Vitamin K_2) Administration on Bone Resorption in Rapidly Progressive Experimental Periodontitis in Rats Reviewed

    KONISHI Hidekazu, KAWANAMI Masamitsu, SAKAGAMI Ryuji, YAMAJI Kozo, WANG Jiaman, KATO Hiroshi

    44 ( 2 )   255 - 264   2001.4

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  • Effects of BMP-2 on bone response using gelatin hydrogel in estrogen-deficient rats Reviewed

    Yamaji Kozo, Yokoyama Akihoto, Matsuzaki Kumoko, Sugaya Tsutomu, Shimada Yasushi, Ohara Naoko, Yoshiyama Masahiro

    J Oral Tissue Engin.   20 ( 2 )   2022

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  • Color Matching Evaluation Using the Visual Analog Scale Reviewed

    Serina ONO, Masahiro YOSHIYAMA, Naoko OHARA, Kumiko MATSUZAKI, Kazuhiko SHIBUYA, Akihito YOKOYAMA, Kei TAKAHASHI, Yasuo SHINNO, Kozo YAMAJI, Yasushi SHIMADA

    The Japanese Journal of Conservative Dentistry   63 ( 1 )   30 - 37   2020

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    <p> Purpose: Recently, the application of composite resin restorations has expanded. Although chromatic studies of composite resins have progressed, color differences measured by colorimeters and visual color evaluation do not always match. The aim of this study was to establish a method of evaluating the visual color of composite resins by using the visual analog scale (VAS), which evaluates subjective data as a continuous variable, and to examine its validity.</p><p> Method: One structural color composite resin (experimental resin : ECM-001) and three commercial composite resins of shade A3 were used in this study. Class Ⅴ and class Ⅲ cavities were prepared in artificial maxillary central incisors of four shades (A1, A2, A3, and A3.5) and each composite resin was filled and its color matching was evaluated on a black-and-white background using VAS. The correlation between VAS value and visual evaluation was analyzed. The color matching of teeth and composite resin were statistically analyzed using one-way analysis of variance and Tukey's HSD test (p<0.05). Background differences in the same sample were analyzed by the t-test (p<0.05).</p><p> Results: In the Class Ⅴ restoration, ECM-001 showed better color matching than other composite resins. In the Class Ⅲ restoration, ECM-001 showed excellent color matching with a wide range of teeth shades on the white background. The VAS values of the other composite resins were higher on the black background than on the white background. However, the VAS value of ECM-001 on the black background tended to decrease. Because the evaluation of VAS color matching by evaluators showed the same tendency, the VAS value reflects information that can be recognized by visual observation. It is suggested that the evaluation of VAS value correlates with visual evaluation.</p><p> Conclusion: It is suggested that the evaluation of color matching of composite resin restorations using VAS is useful for structural color composite resins, providing a new method of visually evaluating composite resin restorations.</p>

    DOI: 10.11471/shikahozon.63.30

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  • Influence of Dentin Desensitizer on Bond Strength of Self-etching Adhesive System Reviewed

    Kumiko MATSUZAKI-TANAKA, Naoko OHARA, Kazuhiko SHIBUYA, Serina ONO, Akihito YOKOYAMA, Kozo YAMAJI, Masahiro YOSHIYAMA

    The Japanese Journal of Conservative Dentistry   60 ( 1 )   22 - 31   2017

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Society of Conservative Dentistry  

    <p> Purpose: Clinically, resin composite repair for dentin hypersensitivity with substantial defects such as wedge-shaped defects is commonly performed a few days after applying the densensitizer to dentin. In this study, we investigated the influence of pre-treatment of dentin densensitizers on bond strength.</p><p> Materials and Methods: We applied three desensitizers (MS coat ONE, F Varnish, and Nanoseal) on an artificial model of hypersensitive dentin using human premolars, and observed the surface structure by scanning electron microscope (SEM). Then, the specimens to which the three desensitizers had been applied were treated with Clearfil Mega Bond FA. We morphologically observed by SEM the surface of dentin which had been treated with the primer. Furthermore, the micro-tensile bond strength (μTBS) for the specimens which were incrementally built up by resin composites was measured in a universal testing machine. The interface between resin and dentin of specimens was observed by SEM and confocal laser scanning microscope (CLSM).</p><p> Results: The surfaces of specimens to which each desensitizer had been applied showed morphological characters having the effects of suppressing hypersensitivity. Regarding μTBS, there were no significant differences among all groups. In the group of MS coat ONE, MS coat ONE was not completely removed by the application of Mega Bond FA primer. Although the penetrations of the primer into dentin tubules were small, the thickness of the bond layer was greater. The specimens of F Varnish showed the functions of self-etching adhesive systems, because the varnishes had been partially removed by Mega Bond FA primer. In the group of Nanoseal, Mega Bond FA primer was almost removed by Nanoseal, and penetrated deeply into the dentinal tubules.</p><p> Conclusion: The three desensitizers used in this study had no effect on the bond strength of self-etching adhesive systems to the artificial model of hypersensitive dentin.</p>

    DOI: 10.11471/shikahozon.60.22

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  • Study of pluripotency and activation of totipotency in human adipose-derived stem cells Reviewed

    Izawa S, Yamaji K, Yokoyama A, Matsuzaki K, Ohara N, Yoshiyama M

    Journal of Oral Tissue Engineering   13 ( 3 )   131 - 135   2016

  • Influence of Incorporating Sodium Fluoride on Flexural Strength, Water Absorption and Solubility of Adhesive Resin Cements Reviewed

    Naoko OHARA, Masahiro YOSHIYAMA, Kazuhiko SHIBUYA, Kumiko TANAKA, Tomohiro HOSHIKA, Azusa ENDO, Maiko NISHIMURA, Akiko TAKEUCHI, Kozo YAMAJI, Yoshihiro NISHITANI

    The Japanese Journal of Conservative Dentistry   58 ( 1 )   10 - 16   2015

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    &emsp;Purpose: By incorporating sodium fluoride in adhesive resin cement, the release of fluoride from the cement is expected to strengthen the teeth and prevent secondary caries. However, there is a concern that such fluoride incorporation and release could impair the physical properties of the cement. In this study, we investigated the influence of incorporating sodium fluoride on the flexural strength, water absorption and solubility of adhesive resin cement.<br>&emsp;Materials and Methods: The experimental resin cements were prepared at concentrations of 0-20 wt% sodium fluoride. The amounts of fluoride released from the cements were measured from day 1 to day 84. Next, a three-point bending test of the experimental resin cements was performed after initial curing (24 hours storage in water at 37&deg;C) and after thermal cycle loading (5&deg;C-55&deg;C, 10,000 times). Furthermore, water absorption and solubility following 30 days immersion in water were measured.<br>&emsp;Results: Fluoride release increased with an increase in the content rate of sodium fluoride. After initial curing, the three-point bending strengths of 5 wt% and 10 wt% sodium fluoride compounded cement were not statistically decreased compared to those of sodium fluoride 0 wt%, but were significantly reduced by incorporation of more than 15 wt%. There were no significant differences in the strength between 15 wt% sodium fluoride compounded cement and 20 wt% sodium fluoride compounded cement. Similar results were also observed in the comparison of flexural strength after thermal cycle loading. Regarding the 30-day water absorption amount, there was no significant difference between 5 wt% sodium fluoride compounded cement and that of 0 wt% sodium fluoride cement. However, the 30-day water absorption of 10 wt% sodium fluoride compounded cement was significantly increased compared to that of 0 wt% sodium fluoride compounded cement. The solubility was not significantly different between the content rate of 0-10 wt% sodium fluoride.<br>&emsp;Conclusion: Increasing the sodium fluoride content improved the amount of fluoride release. On the other hand, the flexural strength of the cement was decreased, and the amounts of water absorption and solubility were increased. Within the limit of this study, it is suggested that 10 wt% sodium fluoride compounded cement could provide fluoride release without decreasing mechanical strength.

    DOI: 10.11471/shikahozon.58.10

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  • 垂直歯根破折歯を口腔外接着再植法にて保存した症例 Reviewed

    大原直子, 田中久美子, 垣内伸子, 星加知宏, 高橋 圭, 神農泰生, 伊澤俊次, 山路公造, 西谷佳浩, 吉山昌宏

    岡山歯学会雑誌   33   7 - 11   2014

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  • Tooth Wear に対してコンポジットレジン修復を行った症例 Reviewed

    田中久美子, 徳善英紀, 大原直子, 星加知宏, 高橋 圭, 神農泰生, 伊澤俊次, 山路公造, 西谷佳浩, 吉山昌宏

    岡山歯学会雑誌   33   1 - 5   2014

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  • Induction of ES cells from Human Bone Marrow Mesenchymal Stem Cells: Epigenetic Control and Initialization of the Cells Reviewed

    Izawa S, Yamaji K, Hoshika T, Nishitani Y, Yoshiyama M

    Journal of Oral Tissue Engineering   11 ( 3 )   220 - 226   2014

  • リコンビナントヒトBMP-2を成体ラットに移植した場合の骨形成に対する低出力超音波パルスの影響 Reviewed

    山路公造, 塩出信太郎, 小田島朝臣, 西谷佳浩, 伊澤俊次, 田中久美, 大原直子, 西村麻衣子, 横山章人, 菅谷 勉, 川浪雅光, 吉山昌宏

    日本歯科保存学会雑誌   56 ( 4 )   318 - 324   2013.8

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    DOI: 10.11471/shikahozon.56.318

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  • Study of Pluripotency and Activation of Totipotency in Human Bone Marrow Mesenchymal Stem Cells Reviewed

    IZAWA Shunji, YAMAJI Kozo, HOSHIKA Tomohiro, NISHITANI Yoshihiro, YOSHIYAMA Masahiro

    Journal of Oral Tissue Engineering   10 ( 2 )   95 - 103   2012

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    Language:English   Publisher:Japanese Association of Regenerative Dentistry  

    Tissue regeneration is the important issue in the field of operative dentistry. We demonstrated in series of studies that human bone marrow mesenchymal stem cells (hMSCs) can differentiate into odontblasts under the certain conditions (Izawa S et al., 2009). Mesenchymal stem cells can differentiate into multiple types of cells derived from mesenchyme (Liechty KW et al., 2000). At the same time odontblasts are primarily derived from mesenchyme; thus, we expected that mesenchymal stem cells can differentiate into odontblasts.The results in series of our study indicated that TNFα is necessary for hMSCs to differentiate into odontblasts and suggest that TNFα activates potential totipotency of hMSCs and the activation can lead to the regeneration of target cells or tissues.

    DOI: 10.11223/jarde.10.95

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  • Bonding of Three Self-etching Priming Adhesive Systems to Bur-cut, Er: YAG-lased and Ultrasonic Vibrated Dentin Reviewed

    NISHITANI Yoshihiro, YOSHIYAMA Masahiro, ITOTA Toshiyuki, HOSHIKA Tomohiro, TAKAHASHI Kei, TANAKA Kumiko, MORIMOTO Sayako, IZAWA Syunji, YAMAJI Kozo, TORII Yasuhiro

    The Japanese Journal of Conservative Dentistry   54 ( 4 )   276 - 288   2011

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    Language:Japanese   Publisher:The Japanese Society of Conservative Dentistry  

    The purpose of this study was to evaluate the effect of the method of removing carious dentin on resin-composite bond strengths to dentin using three self-etching adhesive systems: G-Bond (GC), One-up Bond F (Tokuyama Dental) or Clearfil Mega Bond (Kuraray Medical). The bur-cut, Er: YAG-lased and ultrasonic vibrated dentin was subjected to micro-tensile bond testing and observation of ultra-structure by scanning electron microscopy. A smear layer was not formed on the dentin surface after removing carious dentin in the case of Er: YAG-lased and ultrasonic vibrated dentin. Clearfil Mega Bond showed significantly higher micro-tensile bond strength to bur-cut and ultrasonic vibrated dentin compared with Er: YAG-lased dentin (p<0.05). One-up Bond F showed significantly higher micro-tensile bond strength to ultrasonic vibrated dentin compared with bur-cut and Er: YAG-lased dentin (p<0.05). It is suggested that the method of removing carious dentin by an ultrasonic vibration device is useful for dentin adhesion when using self-etching adhesive systems.

    DOI: 10.11471/shikahozon.54.276

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  • Effects of ultrasound on bone formation induced by BMP-2 Reviewed

    Yamaji K, Nishimura M, Nishitani Y, Izawa S, Hoshika T, Shiode S, Yoshiyama M

    Journal of Oral Tissue Engineering   8 ( 1 )   47 - 51   2010

  • A study of flow on flowable resin composite Reviewed

    HOSHIKA Tomohiro, NISHITANI Yoshihiro, TAKAHASHI Kei, TANAKA Kumiko, NAKATA Takashi, YAMAJI Kozo, YOSHIYAMA Masahiro

    27 ( 3 )   118 - 124   2009.12

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  • Bond Strength and Bio-Compatibility of Experimental Mineralization Accelerating Adhesives Added Collagen Immobilized Poly Ethylene-co-Vinyl Alcohol (EVA+C)

    SHINNO Yasuo, KISHIMOTO Mami, ANABUKI Yuka, YAMAJI Kozo, NISHITANI Yoshihiro, TANAKA Toru, SANO Hidehiko, YOSHIYAMA Masahiro

    Journal of the Japanese Association of Regenerative Dentistry   7 ( 1 )   56 - 63   2009

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    Language:Japanese   Publisher:Japanese Association of Regenerative Dentistry  

    The mineralization accelerating adhesion materials that added EVA+C (RMSB) were produced experimentally, and we studied it about a bond strength and the biocompatibility. As a result, bond strength decreased with increase of the EVA+C addition ratio. But the level of the decrease was inhibited when SBP30 was treated. The RMSB group showed a tendency to growth similar to Control at the early phase, and cell count was higher than an SB (Superbond) at high ratio of EVA+C group. It was a combination of SBP30 and 7P (SB/EVA+C = 40wt%/60wt%) that showed a high bond strength and a good cell proliferation. Also, as a result of animal experiment, 7P had weaker inflammation than an SB and mineralized tissue formation was good. It was suggested that it was obtained high adhesive property and biocompatibility by adding EVA+C in an SB at the appropriate ratio in conclusion.

    DOI: 10.11223/jard.7.56

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  • Dentin Regeneration by the Differentiated Human Bone Marrow Mesenchymal Stem Cells Reviewed

    IZAWA Shunji, YAMAJI Kozo, HOSHIKA Tomohiro, NAKATA Takashi, SHINNO Yasuo, NISHITANI Yoshihiro, YOSHIYAMA Masahiro

    Journal of the Japanese Association of Regenerative Dentistry   7 ( 1 )   47 - 55   2009

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    Language:Japanese   Publisher:Japanese Association of Regenerative Dentistry  

    Dentin regeneration to the lost dentin is the supreme therapy to keep functionality, aesthetics and so forth in operative dentistry. In 2006, we demonstrated the human bone marrow mesenchymal stem cells (hMSCs) differentiated into odontoblast by the stimulation of Emdogain Gel and TNF-&alpha; set. In 2008, we tried to demonstrate odontoblast that differentiated from hMSCs generate dentin under the artificially synthesized appropriate scaffold and molecular signal in vitro. As a result, the differentiated cell tissue tested positive for osteocalcin by fluorescence antibody method and enzyme antibody technique and positive for calcium compounds with the alizarin red S staining. In addition, we detected the dentin-like tissue outside the cell walls by phase contrast microscopy. These results suggested that hMSCs differentiate into odontoblast and generate dentin using Emdogain Gel and TNF-&alpha; set and peptide hydrogel.

    DOI: 10.11223/jard.7.47

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  • Effect of Delayed Application of the Dropped All-in-one Adhesives on Enamel and Dentin Bond Strengths Reviewed

    YAMADA Atsuko, DOI Junichi, NISHITANI Yoshihiro, ITOTA Toshiyuki, YOSHIYAMA Masahiro, TAKABATAKE Yasumitsu, YOSHIMITSU Keiko, TANAKA Kumiko, NOMOTO Masamori, TASHIRO Yoko, OMAE Masanori, YAMAJI Kozo, SHINNO Yasuo

    The Japanese Journal of Conservative Dentistry   50 ( 2 )   126 - 132   2007

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    The purpose of this study was to evaluate the effect of delayed application of the dropped all-in-one adhesives (Clearfil tri-S Bond and G-Bond) on enamel and dentin bond strengths. Human extracted premolars were used and flat enamel and dentin surfaces ground by 600-grit SiC paper were prepared. The tooth surfaces were treated with Clearfil tri-S Bond and G-Bond which were left for 0,5,10 and 30min after dropping of a adhesives on a shaded dish at constant temperature and humidity, and then covered with resin composites according to the manufacturers' instructions. After immersion in 37℃ water for 24 h, the teeth were serially sectioned into multiple slices and the micro-tensile bond strength was measured in a universal testing machine at a crosshead speed of 1.0mm/min. Data were statistically analyzed using one-way ANOVA and Dunnett's test at a significance level of 0.05. The weight changes of the dropped adhesives were also measured until 30 min under the shaded condition. In the comparison of the leaving time, a significant difference in the bond strength was only detected between 0 min and 30 min on enamel. The bond strength of Clearfil tri-S Bond to enamel after 30 min increased, but that of G-Bond after 30 min decreased (p<0.05). There was no significant difference in the bond strengths of Clearfil tri-S Bond and G-Bond to dentin. The weights of Clearfil tri-S Bond and G-Bond decreased with the leaving time after dropping and the percentage of the weights of adhesives were 82.7% for Clearfil tri-S Bond and 52.0% for G-Bond after 30min. These results suggested that the long-term leaving after dropping of the all-in-one adhesives affected the bond strengths to enamel bv evanoration of the solvents in adhesives.

    DOI: 10.11471/shikahozon.50.126

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  • Dopamine Receptor Presence in the Rat Area Postrema Identified by RT-PCR, Immunohistochemistry, and In Situ Hybridization Reviewed

    Izawa Shunji, Yamaai Tomoichiro, Mukudai Yoshiki, Yamaji Kozo, Nishitani Yoshihiro, Itota Toshiyuki, Matsuo Ryuji, Takigawa Masaharu, Yoshiyama Masahiro

    Journal of Oral Biosciences   49 ( 4 )   259 - 268   2007

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    Dopamine (DA) is a major central nervous system (CNS) neurotransmitter with many important physiological activities. Investigations into the neuroanatomy and neurologic functions of the dopaminergic neural systems have generated much debate. Regarding neuroanatomy, physiological and pharmacological criteria have divided DA receptors into D1 and D2 subtypes. The genes encoding these subtypes have been cloned and classified into a D1 subfamily encompassing D1 and D5 receptors and a D2 subfamily with D2, D3, and D4. Based on the sequences of the cloned receptors, we prepared antibodies and riboprobes to elucidate the expression of the corresponding proteins and mRNAs in the rat area postrema (AP) by immunohistochemistry and in situ hybridization (ISH). The AP was obtained from adult male Sprague-Dawley rats undergoing brain surgery, and tissue samples were used for RT-PCR, immunohistochemistry, and ISH. The results showed that D2 and D5 receptors and their mRNAs exist in the rat AP. On the other hand, D1, D3, and D4 receptors and their mRNAs were not detected.

    DOI: 10.2330/joralbiosci.49.259

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  • Bonding of self-etching adhesive systems to demineralized and remineralized dentin Reviewed

    TANAKA Kumiko, DOI Junichi, TAKABATAKE Yasumitsu, NISHITANI Yoshihiro, YAMAJI Kozo, ITOTA Toshiyuki, YOSHIYAMA Masahiro

    Adhesive Dentistry   25 ( 1 )   12 - 18   2007

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    Language:Japanese   Publisher:Japan Society for Adhesive Dentistry  

    This study investigated the bond strengths of one-or two-step self-etching adhesive systems to human sound, demineralized and remineralized dentin. Remineralized dentin was created by remineralization for1or4weeks after demineralization. The dentin surfaces were treated with Clearfil SE Bond or Clearfil Tri-S Bond and the microtensile bond strengths were measured. There were no significant differences in the bond strength of Clearfil SE Bond among the three groups (demineralized, 1-week and4-week remineralized dentin groups) except for the sound dentin group. On the other hand, the bond strength of Clearfil Tri-S Bond to1-week remineralized dentin was significantly higher than those to demineralized dentin and4-week remineralized dentin. In comparing the two adhesive systems, Clearfil SE Bond produced higher bond strengths to all dentin surfaces than Clearfil Tri-S Bond. In conclusion, it is suggested that the difference of the adhesive system affected the bond strength to demineralized and remineralized dentin.

    DOI: 10.11297/adhesdent1983.25.12

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  • Effect of light-curing of the bonding system on bond strength of Clearfil Esthetic Cement-dentin Reviewed

    YOSHIMITSU Keiko, DOI Junichi, TAKABATAKE Yasumitsu, YAMAJI Kozo, ITOTA Toshiyuki, YOSHIYAMA Masahiro

    Adhesive Dentistry   25 ( 1 )   1 - 6   2007

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    This study investigated the effect of light-curing of Clearfil DC Bond (DC Bond) on the micro-tensile bond strength of Clearfil Esthetic Cement to dentin. Extracted human teeth were used in this study. The coronal dentin surfaces were prepared, and treated with DC Bond with or without light-curing, or ED primer II according to the manufacturer's instruction and luted composite block using Clearfil Esthetic Cement. The bond strengths were measured with the micro-tensile bond test. The bond strengths were statistically compared with ANOVA and Duncan's new multiple range tests. The debonded specimens and resin-dentin interfaces were also examined by SEM. There were no significant differences in bond strength among all the groups. In the SEM observations, no morphological differences were found among all the groups. For all fractured surfaces in the debonded DC Bond with light-curing group, cohesive failure in resin was observed. Adhesive fracture was predominantly observed in the debonded DC Bond without light-curing group. The cohesive failure in resin was predominantly observed in the debonded ED primer group.<BR>In conclusion, although the type of fractured surface for the DC Bond without light-curing group differed from that for the DC Bond with light-curing group, there was no significant difference in the dentin bond strength of Clearfil Esthetic Cement between the two groups.

    DOI: 10.11297/adhesdent1983.25.1

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  • Study on Bone Formation Induced by Recombinant Human BMP-2 at Palatal Sites in Adult Rats Reviewed

    YAMAJI Kozo, ITOTA Toshiyuki, IZAWA Shunji, NISHITANI Yoshihiro, YOSHIYAMA Masahiro

    Journal of Oral Tissue Engineering   4 ( 1 )   37 - 42   2006

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    This study was designed to examine the effects of bone formation induced by recombinant human BMP-2 (rhBMP-2), which was implanted together with a poly-lactate-polyglycolate-copolymer/gelatine sponge (PGS) at palatal sites in 30-week- old male Wister rats. The rats were divided into four groups according to the dose of rhBMP-2: 0&micro;g, 4&micro;g, 8&micro;g and 16&micro;g.<BR>New bone formation was observed in every group, and new bone was continuous with the original bone at 6 weeks after implantation. Thickness of new bone (TNB) increased as the dose increased from 0&micro;g to 8&micro;, reached the maximal level at 8&micro;g. In contrast, TNB decreased significantly as the dose increased from 8&micro;g to 16&micro;g.<BR>The results of this study indicate that new bone formation varies according to the dose of rhBMP-2 at palatal sites in adult rats.

    DOI: 10.11223/jarde.4.37

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  • Dentin Regeneration by Direct Pulp Capping Using a Bioabsorbable Material Reviewed

    ITOTA Toshiyuki, HAYASHI Hiroyuki, TASHIRO Yoko, TAKAGI Ryo, YAMAJI Kozo, SHINNO Yasuo, IZAWA Shunji, OMAE Masanori, YOSHIYAMA Masahiro, KOMASA Yutaka

    Journal of Oral Tissue Engineering   4 ( 1 )   17 - 24   2006

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    The aim of this study was to investigate the effect of bioabsorbable material on the direct pulp capping to know whether this material has a function in dentin regeneration by bioabsorption.<BR>Class V cavities with the exposed pulp area were prepared on the buccal surface of first molars in young beagle dogs. All cavities were conditioned with a self-etching primer (Clearfil SE Primer). In the cavities of right side (experimental group), the exposed pulp was covered with a bioabsorbable sheet (Kurabio AG), and then the cavities were coated with an adhesive agent (Clearfil SE Bond) and resorted with a resin composite. The left side cavities (control group) were applied the adhesive agent without the bioabsorbable sheet and restored with a resin composite. On three and six months after surgery, the teeth were extracted, immersion-fixed and decalcified. Afterward, the teeth were frozen-sectioned, stained with hematoxylin and eosin and observed using a light microscopy.<BR>In the experimental group, a large amount of the tertiary dentin was formed into the prepared cavity. On the other hand, the tertiary dentin was almost not formed into the cavity in the control group.<BR>The significantly tertiary dentin formation was recognized into the prepared cavity when the exposed pulp area had been covered by a bioabsorbable material. This result suggested that the space making by a bioabsorbable material could produce the derivation of pulp tissue and then dentin regeneration into the pre-pared cavity.

    DOI: 10.11223/jarde.4.17

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  • Bone Formation Induced by Recombinant Human BMP-2 Combined with Bioabsorbable Material at Subperiosteal Sites Reviewed

    YAMAJI Kozo, NISHITANI Yoshihiro, IZAWA Shunji, SHINNO Yasuo, OMAE Masanori, YOSHIYAMA Masahiro

    Journal of Oral Tissue Engineering   4 ( 2 )   101 - 105   2006

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    The purpose of this study was to evaluate the effect of bone formation induced by recombinant human BMP-2 (rhBMP-2) combined with bioabsorbable material Kurabio&reg;AG (AG) at palatal subperiosteal sites in Wistar rats. AG was freeze-dried alginated gel dressing.<BR>Experimental sites were divided into three groups according to the implans : BMP group (rhBMP-2 combined with AG), AG group (AG alone) and Control group.<BR>In every group, infected region or atypical cell proliferation was not observed, and new bone formation was observed at 6 weeks after implantation. Thickness of new bone (TNB) of BMP group was significantly higher than that of AG and Control groups. There was no significant difference in TNB between AG and Control groups.<BR>These results suggest that rhBMP-2 combined with AG have the ability to promote bone formation, although AG alone do not have any effect on bone formation.

    DOI: 10.11223/jarde.4.101

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  • BMPを用いた歯周組織再生療法

    川浪 雅光, 齋藤 彰, 松本 敦至, 小田島 朝臣, 山路 公造, 宮治 裕史, 加藤 煕

    歯界展望   特別号 ( 健康な心と身体は口腔から-発ヨコハマ2004- )   247 - 247   2005.6

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  • Influence of Streptozotocin-induced Diabetes on New Bone Formation by rhBMP-2 Reviewed

    IWASAKA Kensuke, NEGISHI Jun, YAMAJI Kozo, KAWANAMI Masamitsu

    Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)   46 ( 4 )   266 - 277   2004.12

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    We studied bone formation induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) combined with polylactate-polyglycolate-copolymer/gelatine sponge complexes (PGS) at palatal subperiosteal sites in 54 male Wister rats with STZ-induced diabetes mellitus (DM), insulin-treated STZ-induced diabetes mellitus (INS-DM), or nondiabetes mellitus (nonDM). Rats were divided into 6 groups consisting of PGS implanted alone (nonDM-PGS, DM-PGS, and INS-DM-PGS) and rhBMP-2 combined with PGS implanted (nonDM-BMP, DM-BMP, and INS-DM-BMP). Rats were sacrificed 6 weeks after implantation and histopathologically observaed and histometrically evaluated. New bone formation was observed in all groups, and the new bone was almost completely continuous with the original palatal bone. The DM-PGS group showed significantly less new bone thickness among the 3 groups with PGS implantion alone. NonDM-BMP, DM-BMP and INS-DM-BMP showed significantly greater new bone thickness than did nonDM-PGS, DM-PGS, and INS-DM-PGS groups.<br>These results indicate that rhBMP-2 combined with PGS enhances new bone formation continuous with original bone under STZ-induced diabetes status, although it decreases reparative bone formation.

    DOI: 10.2329/perio.46.266

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  • BMPを用いた歯周組織再生療法

    川浪 雅光, 斎藤 彰, 松本 敦至, 小田島 朝臣, 山路 公造, 宮治 裕史, 加藤 熈

    日本歯科医師会雑誌   57 ( 4 )   386 - 386   2004.7

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  • Effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 combined with fibrous collagen membranes at subperiosteal sites Reviewed

    A Matsumoto, K Yamaji, M Kawanami, H Kato

    JOURNAL OF PERIODONTAL RESEARCH   36 ( 3 )   175 - 182   2001.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MUNKSGAARD INT PUBL LTD  

    This study was designed to examine the effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) combined with a fibrous collagen membrane (FCM). Implantation was done subperiosteally in bilateral palatal grooves in 34 male Wistar rats divided into three age groups: a 10-week-old group(10w group), a 30-week-old group(30w group) and a 70-week-old group (70w group). RhBMP-2-combined FCMs were implanted on the left palatal grooves as BMP-implanted sites (BMP site), while rhBMP-2 was not implanted on the right palatal grooves as control sites. The rats were sacrificed 6 weeks after implantation, and histometric evaluations were performed, New bone formation was observed in every site of each age group and the new bone was almost completely continuous with the original bone. The new bone volume (NBV) of the BMP site was significantly higher than that of the control site in each age group, The NBV of both the control and BMP sites were highest in the 10w group and lowest in the 70w group. The disparity of NBV between the control and BMP sites, which indicated the response to implanted BMP excluding the effect of skeletal growth and surgical stimulation. did not significantly differ among the age groups. These results indicate that rhBMP-2-combined FCM has the ability to induct new bone Formation continuous with original bone even in senescent rats. Furthermore, it appeared that, in the case of palatal subperiosteal implantation, the responsiveness to implanted BMP was independent of age, although the total volume of newly formed bone declined with aging.

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  • Aging-Effect on Bone Formation Induced by Recombinant Human BMP-2 Combined with Polylactate-Polyglycolate-Copolymer/Gelatine Sponge Complexes at Palatal Subperiosteal Sites in Rats Reviewed

    YAMAJI Kozo, MATUSMOTO Atsushi, KATO Hiroshi

    Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)   41 ( 4 )   380 - 391   1999.12

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    Language:Japanese   Publisher:The Japanese Society of Periodontology  

    This study was designed to examine the aging effect on bone formation induced by recombinant human BMP-2 (rhBMP-2) combined with polylactate-polyglycolate-copolymer/gelatine sponge complexes (PGS) at palatal subperiosteal sites (N=72) in 36 Wister strain male rats, at 10 weeks, 30 weeks and 70 weeks of age. The rats at each age were divided into three groups: no implant (CN), PGS implant alone (CI), rhBMP-2 combined with PGS implant (E). The rats were killed at 6 weeks after implantation, and histopathologic observation and histometric evaluation were performed. In histopathologic observation, new bone formation was observed in every group at each age, and the new bone was almost completely continuous with the original palatal bone. In histometric evaluation, the new bone volume was largest in 10-week-old rats and gradually decreased by age in each group. At each age, the new bone volume in group E was significantly larger than that of groups CN and CI. These results indicate that rhBMP-2 combined with PGS induces new bone formation that is continuous with original bone not only in young rats but also in old rats which have less bone formation activity, although the volume of the new bone at the BMP-implant site shows gradual decrease by aging. J. Jpn. Soc. Periodontol., 41: 380-391, 1999.

    DOI: 10.2329/perio.41.380

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Books

  • 生体活性ガラスを添加したボンディング材とマトリックスレジンを用いた脱灰抑制効果

    山路公造, 吉山昌宏( Role: Joint author)

    the Quintessence  2022.6 

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  • ilorane系コンポジットレジンの補修修復における2年間の界面接着耐久性とナノリーケージ

    横山章人, 山路公造, 吉山昌宏( Role: Joint author)

    he Quintessence  2014.8 

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  • 人工唾液中と歯髄内圧シュミレーションを用いた場合のフッ化物含有/非含有モノマー配合接着剤の接着耐久性

    山路公造, 吉山昌宏( Role: Joint author)

    the Quintessence  2014.1 

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  • 岡山大学病院 審美歯科外来

    伊澤俊次, 神農泰生, 山路公造, 西谷佳浩, 吉山昌宏( Role: Joint author)

    歯科審美  2011 

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  • 象牙質再生と再建のハーモナイゼーション

    西谷佳浩, 吉山昌宏, 山路公造( Role: Joint author)

    日本歯科評論  2010 

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  • 保存修復学専門用語集 Glossary of Operative Dentistry 2008 (日本歯科保存学会編)

    ( Role: Joint author)

    医歯薬出版  2008 

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  • 第三の歯科疾患Tooth Wear 原因、予防、対処法と象牙質知覚過敏症との関連(解説)

    中田貴, 山路公造, 西谷佳浩, 吉山昌宏

    歯界展望, 112巻3号  2008 

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  • 歯科臨床研修マニュアル アドバンス編 ひとつ上をめざす研修医のために 第1版 第1刷

    吉山昌宏, 山路公造( Role: Joint author ,  第2章 1.知覚過敏に対する処置)

    永末書店  2007.7 

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  • 増殖因子を用いた象牙質再生療法の開発

    吉山昌宏, 山路公造, 西谷佳浩( Role: Joint author)

    Dental Review No.779 Vol.67(9) 日本歯科評論  2007 

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  • 現代の治療指針 保存・審美修復&カリオロジーと全治療分野編

    クインテッセンス出版,東京  2007 

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  • 象牙質まで及ぶ疾患 いかに必要最小限, かつ十分な処置を行うか

    吉山昌宏, 山路公造( Role: Joint author)

    別冊 the Quintessence Year book 2006 2007 現代の治療指針 保存・審美修復 &カリオロジーと全治療分野 編  2006 

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  • BMPを用いた歯周組織再生療法

    川浪雅光, 齋藤彰, 松本敦至, 小田島朝臣, 山路公造, 宮治裕史, 加藤 熈( Role: Joint author)

    歯界展望(2005特別号)  2005 

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  • ラットの急速に進行する実験的歯周炎における骨吸収に対するメナテトレノン(ビタミンK2)投与による影響

    小西秀和, 川浪雅光, 坂上竜資, 山路公造, 王 佳敏, 加藤 熈( Role: Joint author)

    デンタルダイヤモンド  2001 

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Presentations

  • 低出力超音波パルスがBMP-2配合ゼラチンハイドロゲルを口蓋部に注入した場合の骨形成に及ぼす影響

    山路公造, 横山章人, 松﨑久美子, 大原直子, 吉山知宏, 菅谷 勉, 島田康史, 吉山昌宏

    2021年度日本歯科保存学会秋季学術大会(第155回)  2021.10.28 

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    Event date: 2021.10.28 - 2021.11.10

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  • 新規2ステップシステムにおけるボンド塗布後のエアーブロー圧が象牙質接着性に及ぼす影響

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松﨑久美子, 山路公造, 吉山昌宏

    2021年度日本歯科保存学会秋季学術大会(第155回)  2021.10.28 

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    Event date: 2021.10.28 - 2021.11.10

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  • 新規2ステップボンディング材の象牙質への接着耐久性

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松﨑久美子, 山路公造, 吉山昌宏

    2021年度日本歯科保存学会春季学術大会(第154回)  2021.6.10 

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    Event date: 2021.6.10 - 2021.6.23

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  • エストロゲン欠乏ラットにBMP配合ゼラチンゲルを移植した場合の硬組織反応

    横山 章人, 山路 公造, 吉山 知宏, 大原 直子, 松﨑 久美子, 島田 康史, 吉山 昌宏

    第18回日本再生歯科医学会学術大会  2020.11.28 

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    Event date: 2020.11.28

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 各種レジンコア材料の光照射の有無が象牙質接着強さに及ぼす影響

    大原直子, 井内有人, 小野瀬里奈, 横山章人, 松﨑久美子, 山路公造, 吉山昌宏

    2022年度日本歯科保存学会秋季学術大会(第157回)  2022.11 

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  • 構造色発色フロアブルコンポジットレジンの色調適合性

    小野瀬里奈, 大原直子, 松崎久美子, 澁谷和彦, 横山章人, 高橋圭, 山路公造, 吉山昌宏

    2020年度日本歯科保存学会秋季学術大会(第153回)  2020.11.16 

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  • 塩化ストロンチウム配合象牙質知覚過敏抑制材の脱灰抑制効果

    松﨑久美子, 佐藤奈月, 神農泰生, 山路公造, 大原直子, 島田康史, Sadr Alireza, 角 保徳, 田上順次, 吉山昌宏

    2020年度日本歯科保存学会春季学術大会(第152回)  2020.6.11 

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  • BMP配合アルギン酸ゲルを直接覆髄に用いた場合の硬組織形成

    横山章人, 山路公造, 塩出信太郎, 大原直子, 松﨑久美子, 島田康史, 吉山昌宏

    日本歯科保存学会学術大会(第151回)  2019.11.7 

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  • 頭頚部癌化学放射線療法後に発症した放射線性う蝕の1例

    松﨑久美子, 大原直子, 澁谷和彦, 横山章人, 山路公造, 島田康史, 木股敬裕, 吉山昌宏

    日本歯科保存学会学術大会(第151回  2019.11.7 

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  • Diagnosis of Unopened Occlusal Caries Using Swept-Source Optical Coherence Tomography

    Shimada Y, M Luong, Hosaka K, Yamaji K, Araki K, A Sadr, Miyazaki T, Sumi Y, Tagami J, Yoshiyama M

    97th International Association for Dental Research  2019.6.22 

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  • Influence of Silver Diamine Fluoride on Bond Strengths to Caries affected Dentin.

    Ohara N, Matsuzaki K, Shibuya K, Ono S, Yokoyama A, Yamaji K, Yoshiyama M

    97th International Association for Dental Research  2019.6.22 

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  • Effects of BMP-2 and LIPUS on Bone Formation at Palatal Sites in Aged Rats.

    Yamaji K, Yokoyama A, Shimada Y, Ohara N, Yoshiyama M

    97th International Association for Dental Research  2019.6.22 

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  • 象牙質およびコア用レジンに対するシランカップリング剤含有レジンセメントの 接着強さ

    大原直子, 小野瀬里奈, 澁谷和彦, 横山章人, 松崎久美子, 山路公造, 吉山昌宏

    日本歯科保存学会学術大会第150回  2019.6 

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  • BMP配合ゼラチンゲルをラット口蓋部に移植した場合の硬組織反応

    山路公造, 横山章人, 島田康史, 大原直子, 松崎久美子, 西山依理子, 田畑泰彦, 吉山昌宏

    第16回日本再生歯科医学会学術大会  2018.12.1 

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  • フッ化ジアンミン銀塗布がう蝕象牙質の接着に及ぼす影響

    大原直子, 松崎久美子, 澁谷和彦, 小野瀬里奈, 横山章人, 山路公造, 吉山昌宏

    第149回日本歯科保存学会  2018.11.1 

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  • 化学重合型接着材ボンドマーライトレスを用いたコンポジットレジン修復の窩洞適合性のSS-OCT評価

    横山章人, 山路公造, 島田康史, 松崎久美子, 大原直子, 神農泰生, 高橋 圭, Sadr Alireza, 角保 徳, 田上順次, 吉山昌宏

    第149回日本歯科保存学会  2018.11.1 

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  • Assessment of Surface Contamination of Uncured Composite Resin Using SS-OCT. Invited

    Akihito Yokoyama, Kozo Yamaji, Yasushi Shimada, Alireza Sadr, Yasunori Sumi, Junji Tagami, Masahiro Yoshiyama

    Brazil–Japan Joint Research Workshop on Adhesive Dentistry.  2017.10.31 

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  • スキャホールドが低出力超音波パルス とBMPを用いた骨形成に及ぼす影響

    第14回日本再生歯科医学会学術大会  2017 

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  • Assessment of surface contamination of uncured composite resin using SS-OCT

    OCT International Symposium Okayama  2017 

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  • Assessment of Surface Contamination of Uncured Composite Resin Using SS-OCT

    2017 

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  • Assessment of Surface Contamination of Uncured Composite Resin Using SS-OCT

    Brazil–Japan Joint Research Workshop on Adhesive Dentistry  2017 

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  • 光重合型レジン強化型ケイ酸カルシウム覆髄剤の窩洞洗浄・処理の違いによる封鎖性への影響について

    第147回日本歯科保存学会学術大会  2017 

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  • Optical analysis of dentin de/remineralization from a desensitizer using swept-source optical coherence tomography

    OCT International Symposium Okayama  2017 

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  • Assessment of surface contamination of uncured composite resin using SS-OCT

    OCT International Symposium Okayama  2017 

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  • Optical analysis of dentin de/remineralization from a desensitizer using swept-source optical coherence tomography

    OCT International Symposium Okayama  2017 

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  • 直接覆髄にBMP配合アルギン酸ゲルを用いた場合の硬組織反応

    第15回日本再生歯科医学会学術大会  2017 

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  • 頭頸部がんに対する放射線治療後に多発性のう蝕を発症した一例

    第144回日本歯科保存学会  2016 

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  • セルフアドヒーシブセメント中のMDP濃度が接着強さに及ぼす影響

    第143回日本歯科保存学会  2015 

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  • 加齢が増殖因子を用いた硬組織形成に及ぼす影響

    第13回日本再生歯科医学会学術大会  2015 

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  • 光重合型レジン強化型ケイ酸カルシウム覆髄剤の窩洞洗浄・処理の違いによる封鎖性への影響について

    第143回日本歯科保存学会  2015 

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  • 知覚過敏抑制剤塗布後のレジン-象牙質接着界面の観察

    第143回日本歯科保存学会  2015 

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  • ヒト脂肪由来幹細胞を用いたES細胞の誘導に関する研究―エピジェネティック制御と細胞の初期化―

    第12回日本再生歯科医学会学術大会  2014 

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  • S-PRGフィラー配合試作レジンセメントのフッ素徐放性

    第138回日本歯科保存学会学術大会  2013 

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  • S-PRGフィラー配合試作レジンセメントの象牙質接着性

    第138回日本歯科保存学会学術大会  2013 

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  • 低出力超音波パルスと増殖因子を用いた低侵襲性骨再生療法の開発

    第11回日本再生歯科医学会学術大会  2013 

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  • ヒト骨髄間葉系幹細胞を用いたES細胞の誘導に関する研究―エビジェネティック制御と細胞の初期化―

    第11回日本再生歯科医学会学術大会  2013 

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  • Actinomyces 属3菌種による根面象牙質う蝕の誘発

    第140回日本歯科保存学会  2013 

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  • 細菌バイオミネラリゼーション誘導の試み

    第139回日本歯科保存学会  2013 

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  • 多用途型歯面処理材の象牙質接着強さ

    第139回日本歯科保存学会  2013 

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  • 象牙質知覚過敏抑制材がセルフエッチング接着システムの接着性に及ぼす影響

    第139回日本歯科保存学会  2013 

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  • 歯科用象牙質接着材の細胞毒性と細胞回復度

    第139回日本歯科保存学会  2013 

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  • 新規化学重合型プライマーの象牙質接着性

    第30回日本接着歯学会  2012 

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  • S-PRGフィラー添加が新規レジンセメントの機械的性質に及ぼす影響

    第136回日本歯科保存学会学術大会  2012 

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  • 超音波療法がBMPによる骨形成に及ぼす影響

    第10回日本再生歯科医学会学術大会  2012 

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  • 骨髄間葉系幹細胞を用いたES細胞の誘導に関する研究―免疫賦活因子および初期化因子の探索―

    第10回日本再生歯科医学会学術大会  2012 

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  • コラーゲン固定化エチレン-ビニルアルコール共重合体(EVA+C)添加石灰化誘導促進性接着材の骨に対する親和性

    第10回日本再生歯科医学会学術大会  2012 

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  • 新規多用途型プライマーの歯面処理効果

    第136回日本歯科保存学会学術大会  2012 

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  • 試作象牙質知覚過敏抑材塗布後のレジン接着強さの影響について

    第30回日本接着歯学会  2012 

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  • ヒト骨髄間葉系幹細胞を用いた象牙質・エナメル質複合体の再生に関する研究

    第9回日本再生歯科医学会学術大会  2011 

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  • 直接覆髄にコラーゲン固定化エチレンビニルアルコール共重合体添加石灰化誘導促進性接着剤を用いた象牙質再生療法

    第9回日本再生歯科医学会学術大会  2011 

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  • 歯質表面損失症候群の診断・治療法の開発 人工酸蝕歯の再石灰化とレジン接着性

    第134回日本歯科保存学会学術大会  2011 

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  • ヒト骨髄間葉系幹細胞を用いた象牙質・エナメル質複合体の再生に関する研究 全能性の同定のための遺伝子発現比較分析

    第8回日本再生歯科医学会学術大会  2010 

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  • アスコルビン酸ナトリウム処理が漂白後のエナメル質接着に及ぼす影響

    第28回日本接着歯学会学術大会  2010 

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  • Effects of ultrasound on bone formation induced by BMP-2

    88th International Association for Dental Research  2010 

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  • 浸漬溶液の違いがサーマルサイクル負荷後の象牙質接着強さに及ぼす影響

    第132回日本歯科保存学会学術大会  2010 

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  • 共焦点レーザー顕微鏡を用いたレジン-象牙質接着界面の観察

    第28回日本接着歯学会学術大会  2010 

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  • Effects of ultrasound on bone formation induced by BMP-2

    88th International Association for Dental Research  2010 

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  • オール・イン・ワン・アドヒーシブ前処理がレジンセメントの象牙質接着強さに及ぼす影響

    第132回日本歯科保存学会学術大会  2010 

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  • 歯質界面表面損失症候群の診断・治療法の開発とアンチエイジング歯学の確立

    第133回日本歯科保存学会学術大会  2010 

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  • 新規化学重合型プライマー (DBC-500) の象牙質接着性

    第133回日本歯科保存学会学術大会  2010 

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  • 超音波がBMP骨膜注入後の骨形成に及ぼす影響

    第8回日本再生歯科医学会学術大会  2010 

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  • キトサンオリゴマーを配合した接着性レジンセメントの抗菌性の検討

    第8回日本再生歯科医学会学術大会  2010 

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  • 術者の経験が接着性レジンの象牙質への接着強さに及ぼす影響

    第30回岡山歯学会  2009 

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  • Bond Strength and Bio-Compatibility of Experimental Adhesives Added Collagen Immobilized Poly Ethylene-co-Vinyl Alcohol (EVA+C)

    2009 

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  • SRモノマー配合新規レジンコアシステムの象牙質接着強さ

    第130回日本歯科保存学会春季学術大会  2009 

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  • 術者の経験が接着性レジンの象牙質への接着強さに及ぼす影響

    第130回日本歯科保存学会春季学術大会  2009 

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  • セルフエッチングプライマーによる象牙質中のゼラチン分解酵素の活性化

    第30回岡山歯学会  2009 

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  • BMPによる骨形成に対するCO2レーザーの影響

    第7回日本再生歯科医学会  2009 

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  • ヒト骨髄間葉系幹細胞を用いた象牙質再生に関する研究

    第7回日本再生歯科医学会学術大会  2009 

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  • 新規象牙質知覚過敏抑制材に関する微細形態学的研究

    第131回日本歯科保存学会  2009 

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  • 新規ボンディング材Absolute3の接着性について

    第54回日本歯科理工学会学術講演会  2009 

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  • Bond Strength and Bio-Compatibility of Experimental Adhesives Added Collagen Immobilized Poly Ethylene-co-Vinyl Alcohol (EVA+C)

    The 50th Anniversary of Korean Academy of Conservative Dentistry, 2009 Autumn Scientific Meeting (the 132nd) and The 11th Joint-Scientific Meeting between KACD & JSCD  2009 

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  • 超音波治療とBMP-2を用いた骨再生療法

    第6回日本再生歯科医学会学術大会  2008 

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  • Effects of BMP-2 on bone formation at palatal subperiosteal sites

    86th International Association for Dental Research  2008 

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  • Tooth wearの発症機序の解明とアンチエイジング歯学の展開

    第24回歯科医学を中心とした総合的な研究を推進する集い  2008 

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  • コラーゲン固定化エチレン-ビニルアルコール共重合体(EVA+C)添加試作石灰化 誘導促進性接着材の接着強さと生体親和性に関する基礎的研究

    第129回日本歯科保存学会  2008 

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  • 直接覆髄にrhBMP-2配合アルギン酸ゲルを用いた場合の組織反応

    日本歯科保存大会春季大会(第126回)  2007 

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  • ワンステップボンデイングシステムの歯根部人工脱灰象牙質に対する接着性

    日本歯科理工学会学術講演会  2007 

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  • 生体吸収性材料をスキャホールドとしたリコンビナントヒトBMP-2移植による硬組織形成反応

    第五回日本歯科骨粗鬆症研究会学術大会  2007 

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  • リコンビナントヒトBMP-2配合アルギン酸ゲルを用いた骨再生療法の開発

    第五回日本再生歯科医学会学術大会  2007 

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  • 生体吸収性創傷被覆材kurabio gelに対する象牙質・歯髄複合体の反応

    第四回日本再生歯科医学会学術大会  2006 

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  • アルギン酸ゲルをスキャホールドとしたrhBMP-2移植による組織反応

    第四回日本再生歯科医学会学術大会  2006 

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  • Odontoblast differentiation-dependent gene expression of bone marrow mesenchymal stem cells

    the IADR General Session & Exhibition  2006 

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  • スキャホールドがrhBMP-2による骨形成に及ぼす影響

    岡山歯学会  2006 

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  • リコンビナントヒトBMP-2が若齢と高齢ラットの骨形成に及ぼす影響

    岡山大学歯学部創設25週年記念式典 第25回岡山歯学会総会ならびに学術集会  2005 

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  • 生体吸収性創傷被覆材を用いた象牙質・歯髄複合体の再生

    第三回日本再生歯科医学会学術大会  2005 

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  • 骨粗鬆症マウスにBMP-2を用いた場合の骨形成反応

    日本歯科保存学会2005年度春季学会(第122回)  2005 

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  • Biocompatibility testing of hydroxyapatite using human gingival epithelial cells

    Japanese association for dental research 53rd annual meeting  2005 

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  • リコンビナントヒトBMP-2の濃度がラットの骨形成に及ぼす影響

    日本歯科保存学会2005年度春季学会(第122回)  2005 

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  • 高血糖状態がrhBMP-2による骨形成に及ぼす影響

    第47回春季日本歯周病学会学術大会  2004 

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  • Expression of dopamine receptor mRNA in the rat area postrema

    The 2nd International Dental Science Symposium in Seoul National University and Okayama University  2004 

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  • 歯周病治療へのリコンビナントBMPの応用

    第10回rhBMP-2研究会研究結果報告会  2003 

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  • rhBMP-2による歯周組織形成能に関する研究 -イヌ開窓状欠損周囲を水硬性仮封材で封鎖した場合の組織反応-

    第44回秋季日本歯周病学会学術大会  2001 

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  • 高齢ラットの口蓋部にPGSを担体として移植したrhBMP-2の濃度が骨形成に及ぼす影響.

    第44回秋季日本歯周病学会学術大会  2001 

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  • rhBMP-2による骨形成に対する加齢の影響(弟2報)

    第42回春季日本歯周病学会学術大会  1999 

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  • ラットの実験的歯周炎における破骨細胞と骨吸収に対するビスフォスフォネートの影響

    第111回秋季日本歯科保存学会大会  1999 

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  • チタンインプラント周囲炎の治療にrhBMP-2を応用する試み

    第42回春季日本歯周病学会学術大会  1999 

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Awards

  • 日本再生歯科医学会論文賞

    2012  

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  • 日本再生歯科医学会発表優秀賞

    2011  

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  • The Outstanding Paper Award of J Oral Tissue Engin

    2010  

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  • The 6th JARD Presentation Award

    2008  

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  • The 5th JARD Presentation Award

    2007  

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Research Projects

  • 骨粗鬆症が増殖因子と超音波療法を用いた硬組織形成に及ぼす影響の解明

    Grant number:18K09575  2018.04 - 2021.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    山路 公造, 吉山 昌宏, 菅谷 勉, 島田 康史

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    近年高齢化社会の到来によりわが国において骨粗鬆症が年々増加しつつある。骨粗鬆症には加齢に伴い緩やかな骨量減少が持続する老人性の低回転代謝型、および閉経後のエストロゲン分泌低下により骨吸収が亢進する高回転代謝型が知られている。増殖因子であるBMPは硬組織誘導能を有し象牙質形成や骨形成に関与する因子として知られている。さらに高周波刺激など超音波療法を応用することにより硬組織形成や骨折治癒の促進効果が認められており、骨粗鬆症における検討も必要と考えられる。
    そこで本研究は、増殖因子と超音波療法の硬組織形成反応に着目し、両者を用いた場合の効果について、骨粗鬆症(低回転代謝型および高回転代謝型)が及ぼす影響を解明する目的で実施した。
    本年度は、まず主に低回転型骨粗鬆症における骨代謝低下に伴う硬組織形成の影響を解明する目的として、中高齢の実験動物(ラット)を用いた研究を実施した。その結果、中高齢動物では硬組織(骨)の形成量減少が認められるものの、増殖因子移植により新生骨量は有意に増加した。しかし若齢動物(コントロール)と比べると形成量は有意に減少していた。また超音波療法を用いることにより新生骨は、早期に既存骨と一体化する傾向が認められた。以上より、低回転型骨粗鬆症においては、非骨粗鬆症と比べて硬組織形成の減少が認められるものの、増殖因子により形成量は増加することが示唆された。そして超音波療法を用いると硬組織形成が促進される可能性が示唆された。

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  • Effects of aging on hard tissue formation using Low-intensity pulsed ultrasound and BMP

    Grant number:26462883  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    YAMAJI KOZO

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    This study evaluated the effects of aging on hard tissue formation induced by BMP using Low-intensity pulsed ultrasound(LIPUS)in young and aged rats.
    Tertialy dentin formation was promoted by using pulp capping material contained BMP when LIPUS treatment was performed. There was no significant difference between the young and the aged rat in the volume of tertialy dentin. Newly formed bone was promoted by implantation of material contained BMP at palatal subperiosteal site when LIPUS treatment was performed, although the volume of bone decreased with aging. These results suggest that the effects of aging on hard tissue formation might vary with the experimental sites.

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  • Development of dentin regeneration using growth factor and low-intesity pulsed ultasoud

    Grant number:23592797  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    YAMAJI KOZO, YOSHIYAMA Masahiro, NISHITANI Yoshihiro, IZAWA Shunji

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    The purpuse of this study was to evaluate the effects of dentin regeneration(formation) using growth factor (BMP; Bone Morphogenetic Protein) and low-intesity pulsed ultasoud(LIPUS) on direct pulp capping. Tertiary dentin formation was promoted by using pulp capping material contained BMP in comparison with non-BMP. Furthermore, it was enhanced by the application of LIPUS.
    These results suggest that the combination of growth factor (BMP) and LIPUS has the ability to enhance dentin formation in the case of direct pulp capping.

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  • Study on regeneration of dentin-enamel complex by the bone marrow mesenchymal stem cells

    Grant number:21592420  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    IZAWA Shunji, YOSHIYAMA Masahiro, NISHITANI Yoshihiro, YAMAJI Kozo

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

    Every cell can differentiate into target cell and regenerate target tissue via embryo-stem cell under the condition that RNA polymeraseIIthat takes on first step transcription of gene expression, change from free state to systematized state by TNFαwhich is typical inflammatory cytokine and appropriate activation factor and organizational environment factor(scaffold, differentiation factor, culture media).

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  • 歯質表面損失症候群の診断・治療法の開発とアンチエイジング歯学の確立

    Grant number:21659438  2009 - 2010

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    吉山 昌宏, 西谷 佳浩, 山路 公造

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    Grant amount:\3000000 ( Direct expense: \3000000 )

    う蝕、歯周病に次ぐ第3の歯科疾患として注目されているTooth Wearは実に成人の80%以上が罹患していると言われており、大きく咬耗・摩耗・酸蝕症の3つに分類されている。さらに近年、Tooth surface loss(TSL,歯質表面損失症候群)という非う蝕性の表面歯質アパタイトの慢性的な損失を示した概念が発表された。TSLにはTooth Wearのほかにアブフラクションや歯根露出も含まれ、国民の注目を集めている。H21年度には、デジタルマイクロスコープを用いてヒト抜去歯の咬耗面を観察し、その表面積を測定した。H22年度には、人工酸蝕歯を用いて唾液による再石灰化能を検討するとともに、ワンステップボンディングシステムの人工酸蝕象牙質への接着性を検討した。健全ヒト抜去大臼歯の歯冠部咬合面象牙質平坦面を露出後、コーラ系炭酸飲料に5分間浸漬し、その後、ヒト唾液中に浸漬し、3分後および5分毎にヌープ硬さを計測しながら45分後まで測定した。コーラ浸漬後の露出象牙質面の硬さを100とした場合、唾液浸漬3分後には、ヌープ硬さが有意に上昇し、45分後には硬さは112まで上昇し、ヌープ硬さの平均値は健全部と同等の62.6を示した。また微小引張試験の結果から健全象牙質に対し、ビューティーボンドは35.5±1.51MPa(n=10)を示したのに対して、人工酸蝕象牙質では27.1±1.86MPaと、有意に低い値を示した。ヒト抜去歯の象牙質露出面は、コーラなどの酸性飲料により大きくヌープ硬さが減弱することが示されたが、唾液浸漬45分でほぼ健全な硬さにまで回復することが示された。この結果は、歯根露出した患者の歯磨き方法に大きな示唆を与えると考えられる。また、現在、疫学倫理委員会の承認の下に、デジタルマイクロスコープを用いた咬耗歯の疫学調査を継続中である。

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  • Regeneration of dentin and alveolar bone using growth factors and ultrasound

    Grant number:20592225  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    YAMAJI Kouzou, YOSHIYAMA Masahiro, NISHITANI Yoshihiro, ODAJIMA Tomoomi, KAWANAMI Masamitsu

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    The purpose of this study was to evaluate the effect of dentin and bone regeneration using growth factor and ultrasound. As a result, the dentin formation was promoted by using the growth factor in the case of direct pulp capping. Newly formed bone was observed at subperiosteal sites using growth factor. Furthermore, the maturation of newly formed bone was enhanced by using ultrasound. These results suggest that growth factor and ultrasound have the ability to enhance hard tissue formation.

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  • Application of Nanofioscience to Dentin Regenerative Theraysy

    Grant number:19390484  2007 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    YOSHIYAMA Masahiro, NISHITANI Yoshihiro, YAMAJI Kozo, IZAWA Shunji, SHINNO Yasuo, DOI Junichi, OHMAE Masanori, SANO Hidehiko

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    Grant amount:\12740000 ( Direct expense: \9800000 、 Indirect expense:\2940000 )

    The mineralization accelerating adhesion materials that added EVA+C (RMSB) were produced experimentally, and we studied the bond strength and biocompatibility. Bond strength decreased with increase of the EVA+C addition ratio. The RMSB group showed a tendency to growth similar to Control at the early phase, and cell count was higher than an SB (Superbond) at high ratio of EVA+C group. SBP30 and 7P (SB/EVA+C=40wt%/60wt%) showed a high bond strength and a good cell proliferation. Invivo, 7P had weaker inflammation than an SB and mineralized tissue formation was good. High adhesive property and biocompatibility was oftained by RMSB-7P.

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  • Clinical training: Advanced Esthetic Dentistry (2022academic year) special  - その他

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  • Clinical metal-based materials (2022academic year) Second semester  - 木1,木2

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  • Practices of cavity preparation, filling technique and metal inlay restoration (2021academic year) 2-4 semesters  - [第2学期]金1,金2,金3, [第3学期]金5,金6,金7, [第4学期]金5,金6,金7

  • Clinical training: Advanced Esthetic Dentistry (2021academic year) special  - その他

  • Lecture and Research Projects: Advanced Esthetic Dentistry (2021academic year) special  - その他

  • Clinical training: Advanced Adhesive Dentistry (2021academic year) special  - その他

  • Lecture and Research Projects: Advanced Adhesive Dentistry (2021academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry I (2021academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry I (2021academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry II (2021academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry II (2021academic year) special  - その他

  • Clinical metal-based materials (2021academic year) Second semester  - 木1,木2

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  • Cariology and Clinical adhesive materials (2021academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

  • Cariology and Clinical adhesive materials (2021academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

  • Practices of cavity preparation, filling technique and metal inlay restoration (2020academic year) 2-4 semesters  - [第2学期]金1,金2,金3, [第3学期]金5,金6,金7, [第4学期]金5,金6,金7

  • Practices of cavity preparation, filling technique and metal inlay restoration (2020academic year) 2-4 semesters  - [第2学期]金1,金2,金3, [第3学期]金5,金6,金7, [第4学期]金5,金6,金7

  • Clinical training: Advanced Esthetic Dentistry (2020academic year) special  - その他

  • Lecture and Research Projects: Advanced Esthetic Dentistry (2020academic year) special  - その他

  • Clinical training: Advanced Adhesive Dentistry (2020academic year) special  - その他

  • Lecture and Research Projects: Advanced Adhesive Dentistry (2020academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry I (2020academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry I (2020academic year) special  - その他

  • Research Projects and Practicals:Operative Dentistry II (2020academic year) special  - その他

  • Lecture and Research Projects: Operative Dentistry II (2020academic year) special  - その他

  • Clinical metal-based materials (2020academic year) Second semester  - 木1,木2

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  • Cariology and Clinical adhesive materials (2020academic year) 1st and 2nd semester  - [第1学期]木1,木2, [第2学期]金4

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