Updated on 2021/12/16

写真a

 
MIYAISHI Satoru
 
Organization
Medicine, Dentistry and Pharmaceutical Sciences Professor
Position
Professor
External link

Degree

  • PhD ( Okayama University )

Research Interests

  • Legal Medicine

  • Forensic Pathology

  • immunoassay

  • myoglobin

Research Areas

  • Life Science / Forensics medicine

Education

  • Okayama University   医学研究科   法医学

    - 1991.3

      More details

    Country: Japan

    researchmap

  • Okayama University   医学部   医学科

    - 1986.3

      More details

    Country: Japan

    researchmap

Research History

  • Dept Legal Medicine,Okayama University   Professor

    2007.4

      More details

  • Guest Professor, Institute of Legal Medicine, University of Hamburg, Germany

    2003

      More details

  • Guest Professor,Institute of Legal Medicine, University of Hamburg

    1997

      More details

  • ハンブルク大学法医学研究所 客員研究員

    1995

      More details

Professional Memberships

Committee Memberships

  • 日本法医学会   日本法医学雑誌副編集委員長  

    2017 - 2019   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

  • 日本法医学会   副広報渉外委員長  

    2017 - 2019   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

  • 日本SIDS・乳幼児突然死予防学会会   評議員  

    2017   

      More details

    Committee type:Academic society

    日本SIDS・乳幼児突然死予防学会会

    researchmap

  • 日本SIDS・乳幼児突然死予防学会会   理事  

    2017   

      More details

    Committee type:Academic society

    日本SIDS・乳幼児突然死予防学会会

    researchmap

  • 日本SIDS・乳幼児突然死予防学会会   症例検討委員  

    2017   

      More details

    Committee type:Academic society

    日本SIDS・乳幼児突然死予防学会会

    researchmap

  • 日本SIDS・乳幼児突然死予防学会会   副症例検討委員長  

    2017   

      More details

    Committee type:Academic society

    日本SIDS・乳幼児突然死予防学会会

    researchmap

  • 日本法医学会   理事  

    2015 - 2019   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

  • 日本法医学会   日本法医学雑誌編集委員長  

    2015 - 2017   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

  • 日本法医学会   広報渉外委員長  

    2015 - 2017   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

  • 日本法医学会   日本法医学会創立100周年記念誌編集委員長  

    2013 - 2015   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

  • 法医病理研究会   会長  

    2012 - 2015   

      More details

    Committee type:Academic society

    法医病理研究会

    researchmap

  • ドイツ語圏法医学会   準名誉会員  

    2012   

      More details

    Committee type:Academic society

    ドイツ語圏法医学会

    researchmap

  • 日本SIDS・乳幼児突然死予防学会会   会員  

    2007   

      More details

    Committee type:Academic society

    日本SIDS・乳幼児突然死予防学会会

    researchmap

  • 日本法医学会   評議員  

    1994   

      More details

    Committee type:Academic society

    日本法医学会

    researchmap

▼display all

 

Papers

  • 災害時の多数死体検案-平成 30 年 7 月豪雨における岡山大学法医学分野の活動. Reviewed

    三浦雅布, 谷口 香, 山﨑雪恵, 小林智瑛, ThuThuHtike, 山本雄二, 宮石 智

    法医病理   27 ( 2 )   2021.12

     More details

    Authorship:Last author  

    researchmap

  • ミャンマー国における法医学活動の現況 Reviewed

    Forensic Pathology   27 ( 1 )   83 - 90   2021.6

     More details

    Authorship:Last author  

    researchmap

  • Postmortem Diagnosis of Fulminant Type 1 Diabetes Mellitus: Case Report Reviewed

    SN Comprehensive Clinical Medicine   2021.4

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s42399-021-00921-4

    researchmap

    Other Link: https://link.springer.com/article/10.1007/s42399-021-00921-4/fulltext.html

  • 直接死因として急性覚せい剤中毒死が疑われた肺脂肪塞栓症による死亡の一例 Reviewed

    吉留 敬, 守屋 文夫, 三浦 雅布, 宮石 智

    法医学の実際と研究   ( 63 )   29 - 33   2020.11

     More details

    Authorship:Last author   Language:Japanese   Publisher:法医学談話会  

    症例は30歳代男性で、アパート3階の自宅から飛び降り、動けなくなったため救急要請がなされた。警察官現着時には男性は担架の上で大暴れしていたが、搬送を開始する頃に急におとなしくなった。病院到着時には見当識の異常などがみられる程度であった。しかし、わずか数分のうちに口から泡を吹くなど急速に容態が悪化し、到着の約2時間後に死亡が確認された。CT他各種検査の結果からも死因となる損傷・病変は認められず、死因究明のため翌日に司法解剖が行われた。急性覚せい剤中毒死が強く疑われたが、ガスクロマトグラフィー質量分析計を用いた定量分析の結果、血中メタンフェタミン濃度は1.00〜1.32μg/mLであり、致死濃度を下回っていた。その後、病理組織検査の結果、肺血管に多数の脂肪塞栓が認められ、第2腰椎骨折に続発する肺脂肪塞栓症による死亡であることが明らかとなった。

    researchmap

  • Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung. Reviewed International journal

    Munenori Habuta, Akihiro Yasue, Ken-Ichi T Suzuki, Hirofumi Fujita, Keita Sato, Hitomi Kono, Ayuko Takayama, Tetsuya Bando, Satoru Miyaishi, Seiichi Oyadomari, Eiji Tanaka, Hideyo Ohuchi

    PloS one   15 ( 10 )   e0240333 - e0240333   2020.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Public Library of Science (PLoS)  

    CRISPR/Cas9-mediated gene editing often generates founder generation (F0) mice that exhibit somatic mosaicism in the targeted gene(s). It has been known that Fibroblast growth factor 10 (Fgf10)-null mice exhibit limbless and lungless phenotypes, while intermediate limb phenotypes (variable defective limbs) are observed in the Fgf10-CRISPR F0 mice. However, how the lung phenotype in the Fgf10-mosaic mutants is related to the limb phenotype and genotype has not been investigated. In this study, we examined variable lung phenotypes in the Fgf10-targeted F0 mice to determine if the lung phenotype was correlated with percentage of functional Fgf10 genotypes. Firstly, according to a previous report, Fgf10-CRISPR F0 embryos on embryonic day 16.5 (E16.5) were classified into three types: type I, no limb; type II, limb defect; and type III, normal limbs. Cartilage and bone staining showed that limb truncations were observed in the girdle, (type I), stylopodial, or zeugopodial region (type II). Deep sequencing of the Fgf10-mutant genomes revealed that the mean proportion of codons that encode putative functional FGF10 was 8.3 ± 6.2% in type I, 25.3 ± 2.7% in type II, and 54.3 ± 9.5% in type III (mean ± standard error of the mean) mutants at E16.5. Histological studies showed that almost all lung lobes were absent in type I embryos. The accessory lung lobe was often absent in type II embryos with other lobes dysplastic. All lung lobes formed in type III embryos. The number of terminal tubules was significantly lower in type I and II embryos, but unchanged in type III embryos. To identify alveolar type 2 epithelial (AECII) cells, known to be reduced in the Fgf10-heterozygous mutant, immunostaining using anti-surfactant protein C (SPC) antibody was performed: In the E18.5 lungs, the number of AECII was correlated to the percentage of functional Fgf10 genotypes. These data suggest the Fgf10 gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung. Since dysfunction of AECII cells has been implicated in the pathogenesis of parenchymal lung diseases, the Fgf10-CRISPR F0 mouse would present an ideal experimental system to explore it.

    DOI: 10.1371/journal.pone.0240333

    Web of Science

    PubMed

    researchmap

  • 蝶形骨洞内貯留液中の珪藻の生細胞染色による検査法の淡水溺死診断への応用(Application of Vital Staining of Diatoms in the Sphenoid Sinus Fluid for the Forensic Diagnosis of Freshwater Drowning) Reviewed

    山本 雄二, 三浦 雅布, 宮石 智

    犯罪学雑誌   86 ( 3 )   82 - 91   2020.9

     More details

    Authorship:Last author   Language:English   Publisher:日本犯罪学会  

    淡水環境での溺死者に観察される蝶形骨洞内貯留液から、植物性プランクトンである珪藻の生細胞を検出するため、ニュートラルレッド(NR)法を用いた生細胞染色により、生死細胞判別を試みた。岡山県内の淡水域、河川水、溝渠、用水路、池、ダム湖から収集した水試料をNR試液で染色後、顕微鏡観察を行った。その結果、珪藻から生細胞が簡便な操作で30分以内に検出可能であることが立証されたたため、次に、2014年9月〜2017年5月までの期間内に、当法医学教室で行われた剖検のうち、淡水環境での溺死が示唆された溺死体15体と非溺死体5体を用いて実施した。蝶形骨洞内貯留液から試料を採取し、NR染色による珪藻生細胞の検出を行った結果、溺死体15体中12体に、蝶形骨洞内貯留液から淡水産プランクトン珪藻の生細胞が検出された。また、発見時に死後長時間経過していた2体からは珪藻細胞の死細胞のみが検出されたが、浴槽で発見された1体と非溺死体の蝶形骨洞内貯留液からは、珪藻細胞は検出されなかった。以上の結果から、本手法により、淡水環境での水吸引による溺死の判定が可能になることが判明した。

    researchmap

  • Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla. Reviewed International journal

    Munenori Habuta, Hirofumi Fujita, Keita Sato, Tetsuya Bando, Junji Inoue, Yoichi Kondo, Satoru Miyaishi, Hiromi Kumon, Hideyo Ohuchi

    Cell and tissue research   379 ( 1 )   157 - 167   2020.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    REIC (reduced expression in immortalized cells) has been identified as a gene whose expression was reduced in immortalized cultured cells. The REIC gene is identical to Dickkopf-3 (Dkk3), which encodes a secreted glycoprotein belonging to the Dkk family. Previously, we showed that Dkk3 protein is present in the mouse adrenal medulla. However, its role in this tissue has not been elucidated. To explore it, we performed electron microscopic (EM) studies and RNA-sequencing (RNA-seq) analysis on Dkk3-null adrenal glands. EM studies showed that the number of dense core secretory vesicles were significantly reduced and empty vesicles were increased in the medulla endocrine cells. Quantitative PCR (qPCR) analysis showed relative expression levels of chromogranin A (Chga) and neuropeptide Y (Npy) were slightly but significantly reduced in the Dkk3-null adrenal glands. From the result of RNA-seq analysis as a parallel study, we selected three of the downregulated genes, uncoupled protein-1 (Ucp1), growth arrest and DNA-damage-inducible 45 gamma (Gadd45g), and Junb with regard to the estimated expression levels. In situ hybridization confirmed that these genes were regionally expressed in the adrenal gland. However, expression levels of these three genes were not consistent as revealed by qPCR. Thus, Dkk3 maintains the integrity of secreting vesicles in mouse adrenal medulla by regulating the expression of Chga and Npy.

    DOI: 10.1007/s00441-019-03113-8

    Web of Science

    PubMed

    researchmap

  • 法医病理アトラス 縊死(2) Reviewed

    谷口 香, 三浦 雅布, 小林 智瑛, 宮石 智

    法医病理   25 ( 2 )   115 - 116   2020.1

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 法医病理アトラス 精液検査 Reviewed

    小林 智瑛, 三浦 雅布, Thu Thu Htike, 谷口 香, 竹居 セラ, 高田 智世, 宮石 智

    法医病理   25 ( 2 )   117 - 118   2020.1

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • Has the mortality rate of sudden infant death syndrome been decreasing in Japan? : an approach using vital statistics for analyzing practical situation of sudden infant death Reviewed

    19 ( 1 )   7 - 13   2019.12

     More details

    Authorship:Last author   Language:Japanese  

    CiNii Article

    researchmap

  • Cyanide concentrations in blood and tissues of fire victims. Reviewed International journal

    Thu Thu Htike, Fumio Moriya, Satoru Miyaishi

    Legal medicine (Tokyo, Japan)   41 ( 3 )   101628 - 101628   2019.11

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    Cyanide poisoning has been regarded to contribute the fatal outcome in fire victims. The toxicity of inhaled hydrogen cyanide (HCN) at the cellular level was evaluated considering the impact of methemoglobin (MetHb) produced by fire gases. Cyanide (CN) concentrations and total hemoglobin contents were measured in right heart blood (RHB) and seven organs/tissues (basal ganglia, brain stem, heart, lung, liver, kidney and psoas muscle) collected from 20 fire fatalities. MetHb and carboxyhemoglobin saturations were also measured in RHB. The amount of CN probably bound to the cytochrome c oxidase of the tissue cells (CCO-CN) was extrapolated from CN and hemoglobin contents in RHB and organs/tissues, MetHb saturation in RHB and binding capacity of MetHb for CN. CN concentrations in RHB showed a wide range with the highest concentration of 8.927 μg/mL. The lung contained the largest CN content among organs/tissues with the mean concentration of 2.219 μg/g, then the heart (0.259 μg/g) and it was lower than 0.100 μg/g in others. Exceedingly large amount of CN in the lung could be explained by high hemoglobin content, being the port of entry of HCN and postmortem diffusion of fire gases. CCO-CN was theoretically present in about 20% of organ/tissue samples, most commonly in the basal ganglia (10 samples, with the mean of 0.059 μg/g) followed by heart (eight samples, with the mean of 0.109 μg/g). No CCO-CN was found in liver and kidney. HCN might have the effect on brain and heart.

    DOI: 10.1016/j.legalmed.2019.101628

    Web of Science

    PubMed

    researchmap

  • Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye. Reviewed International journal

    Mutsuko Kato, Keita Sato, Munenori Habuta, Hirofumi Fujita, Tetsuya Bando, Yuki Morizane, Fumio Shiraga, Satoru Miyaishi, Hideyo Ohuchi

    Biochemistry and biophysics reports   19   100665 - 100665   2019.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER  

    Recent studies show that exposure to ultraviolet (UV) light suppresses ocular elongation, which causes myopia development. However, the specific mechanisms of this process have not been elucidated. A UV-sensor, Opsin 5 (Opn5) mRNA was shown to be present in extraretinal tissues. To test the possibility that UV-signals mediated by Opn5 would have a direct effect on the outer connective tissues of the eye, we first examined the expression patterns of a mammalian type Opn5 (Opn5m) in the late-embryonic chicken eye. Quantitative PCR showed Opn5m mRNA expression in the cornea and sclera. The anti-Opn5m antibody stained a small subset of cells in the corneal stroma and fibrous sclera. We next assessed the effect of UV-A (375 nm) irradiation on the chicken fibroblast cell line DF-1 overexpressing chicken Opn5m. UV-A irradiation for 30 min significantly increased the expression of Early growth response 1 (Egr1), known as an immediate early responsive gene, and of Matrix metalloproteinase 2 (Mmp2) in the presence of retinal chromophore 11-cis-retinal. In contrast, expression of Transforming growth factor beta 2 and Tissue inhibitor of metalloproteinase 2 was not significantly altered. These results indicate that UV-A absorption by Opn5m can upregulate the expression levels of Egr1 and Mmp2 in non-neuronal, fibroblasts. Taken together with the presence of Opn5m in the cornea and sclera, it is suggested that UV-A signaling mediated by Opn5 in the extraretinal ocular tissues could influence directly the outer connective tissues of the chicken late-embryonic eye.

    DOI: 10.1016/j.bbrep.2019.100665

    Web of Science

    PubMed

    researchmap

  • Site specificity and effects of antemortem and postmortem blood properties on postmortem blood methamphetamine concentrations: a report of 7 cases. Reviewed

    Yoshitome K, Moriya F and Miyaishi S:

    Acta Criminologiae et Medicinae Legalis Japonica,   85 ( 3 )   73 - 77   2019.9

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)  

    researchmap

  • Unintentional Injury Deaths among Children: A Descriptive Study Using Medico-legal Documents in Okayama Prefecture, Japan (2001-2015). Reviewed

    Yukie Yamasaki, Nanako Tamiya, Hideki Yamamoto, Satoru Miyaishi

    Acta medica Okayama   73 ( 2 )   117 - 125   2019.4

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    According to the World Health Organization's World Report, approx. 950,000 children and young people < 18 years old die from an injury each year, and unintentional injury deaths account for a large portion of these cases. Here we used medico-legal documents to epidemiologically analyze the cases of unintentional injury deaths among children < 5 years old in Okayama Prefecture, Japan from 2001 to 2015. Age, sex, manner/cause of death, and various circumstances of the incident were investigated. There were 73 unintentional injury deaths during the study period. Drowning (n=29), suffocation (n=24), and transport accidents (n=13) were the major categories of unintentional injury deaths. Twenty-two cases (30.1%) were autopsied. Differences in the characteristics of the unintentional injury deaths by age were observed. Information which cannot be obtained from Vital Statistics was available from medico-legal documents, and detailed characteristics of unintentional injury deaths among children < 5 years old were elucidated. Investigating medico-legal information is one of the meaningful measures for the prevention of unintentional injury deaths among children in Japan.

    DOI: 10.18926/AMO/56647

    Web of Science

    PubMed

    researchmap

  • The estimation of the occupational death due to the agricultural work based on the data base of Japanese occupational death and demographic statistics of Japan Reviewed

    5 ( 1 )   60 - 65   2019.3

     More details

    Language:Japanese  

    CiNii Article

    researchmap

  • 法医病理アトラス 縊死 Reviewed

    谷口 香, 三浦 雅布, 宮石 智

    法医病理   24 ( 2 )   161 - 162   2018.12

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 法医病理アトラス 硫化水素中毒 Reviewed

    谷口 香, 三浦 雅布, 吉留 敬, 山崎 雪恵, 宮石 智

    法医病理   24 ( 1 )   66 - 67   2018.6

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 法医病理アトラス 腐敗網 Reviewed

    三浦 雅布, 谷口 香, 山崎 雪恵, Thu Thu Htike, 小林 智瑛, 宮石 智

    法医病理   23 ( 2 )   91 - 92   2017.12

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 法医病理アトラス ワイドラー徴候 Reviewed

    木下 博之, 田中 直子, 高倉 彩華, モストファ・ジャーマル, 伊藤 明日香, 木村 正司, 筒井 邦彦, 松原 修司, 三浦 雅布, 宮石 智

    法医病理   23 ( 2 )   93 - 93   2017.12

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 法医病理アトラス 穿孔骨折 Reviewed

    谷口 香, 三浦 雅布, 宮石 智

    法医病理   23 ( 2 )   94 - 95   2017.12

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • Gastric excretion of intravenously administered drugs in critical care patients. Reviewed International journal

    Fumio Moriya, Kei Yoshitome, Satoru Miyaishi

    Legal medicine (Tokyo, Japan)   23   77 - 78   2016.11

     More details

    Authorship:Last author   Language:English   Publisher:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.legalmed.2016.10.002

    Web of Science

    PubMed

    researchmap

  • Autopsy Case of Bilateral Optic Nerve Aplasia with Microphthalmia: Neural Retina Formation Is Required for the Coordinated Development of Ocular Tissues. Reviewed

    Hideyo Ohuchi, Kaori Taniguchi, Satoru Miyaishi, Hitomi Kono, Hirofumi Fujita, Tetsuya Bando, Chiharu Fuchizawa, Yuko Ohtani, Osamu Ohtani

    Acta medica Okayama   70 ( 2 )   131 - 7   2016.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    Human congenital anomalies provide information that contributes to the understanding of developmental mechanisms. Here we report bilateral optic nerve aplasia (ONA) with microphthalmia in the autopsy of the cadaver of a 70-year-old Japanese female. The gross anatomical inspection of the brain showed a cotton thread-like cord in the presumed location of the optic nerve tract or chiasm. Histologically, no neural retina, optic nerve bundle or retinal central vessels were formed in the eye globe, and the retinal pigment cells formed rosettes. The cornea, iris, and lens were also histologically abnormal. Immunohistochemically, no retinal cells expressed beta III tubulin, and Pax6-immunoreactive cells were present in the ciliary non-pigmented epithelial cells. This case of ONA could be attributed to the agenesis of retinal projection neurons as a sequel to the disruption of neural retina development. The neural retina formation would coordinate the proper development of ocular tissues.

    DOI: 10.18926/AMO/54192

    Web of Science

    PubMed

    researchmap

  • Sudden infant death due to Lactococcal infective endocarditis. Reviewed International journal

    K Taniguchi, M Nakayama, K Nakahira, Y Nakura, N Kanagawa, I Yanagihara, S Miyaishi

    Legal medicine (Tokyo, Japan)   19   107 - 11   2016.3

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    Infective endocarditis (IE) of infants is rare, most of which occur associated with congenital heart disease or its cardiac surgery. We experienced a case of sudden death of a four-month-old male infant without congenital heart disease. It was elucidated by postmortem examination that the dead had suffered severe IE, which led him to death. In the microbiological genetic analysis using histological section, the pathogen causing inflammation in the present case was identified as Lactococcus lactis subspecies, although Staphylococci have been reported to be common and important one. Previously reported infectious diseases by Lactococcus lactis subspecies were all adult cases and this is the first report of an infantile death due to Lactococcal IE according to our knowledge. Any fatal disease may be included in sudden death cases targeted for forensic autopsy, even if it is rare. It is expected for forensic pathologists that they note such case and share each experience among themselves and other medical fields to develop a strategy for prevention. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.legalmed.2015.07.013

    Web of Science

    PubMed

    researchmap

  • Measurement of Postmortem 1,5-anhydroglucitol in Vitreous Humor for Forensic Diagnosis. Reviewed International journal

    Tomoyo Takata, Yukie Yamasaki, Takashi Kitao, Satoru Miyaishi

    Journal of forensic sciences   61 Suppl 1   S150-3 - S153   2016.1

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    In forensic diagnosis, postmortem blood glucose is known to be susceptible to change after death. However, the 1,5-anhydroglucitol (1,5-AG) concentrations in plasma and cerebrospinal fluid (CSF) reflect the mean blood glucose level for a short period of time. In this study, we compared the postmortem 1,5-AG concentrations in vitreous humor and CSF in 47 subjects to evaluate the utility of this concentration in the vitreous humor for forensic diagnosis. The postmortem 1,5-AG concentrations in vitreous humor (mean +/- SD: 20.2 +/- 8.7 mu g/mL) and CSF (16.8 +/- 8.7 mu g/mL) did not differ significantly and showed a strong correlation (r(2) = 0.87, p &lt; 0.01). These results suggest that the vitreous humor 1,5-AG concentration provides useful information on the antemortem blood glucose level, in addition to the HbA1c value and the CSF 1,5-AG concentration.

    DOI: 10.1111/1556-4029.12963

    Web of Science

    PubMed

    researchmap

  • 乳幼児の法医解剖 Reviewed

    宮石智, 谷口香, 三浦雅布

    日本SIDS・乳幼児突然死予防学会   16 ( 1 )   8 - 14   2016

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:日本SIDS・乳幼児突然死予防学会  

    CiNii Article

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/2017307937

  • 法医解剖例からみた乳児急死の実態 Reviewed

    石原万実, 谷口香, 宮石智, 守屋文夫

    法医病理   22 ( 2 )   69 - 73   2016

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本法医病理学会  

    乳児の予期しない急死の予防に資する目的で、2008年〜2015年に岡山大学大学院医歯薬学総合研究科法医学分野において解剖鑑定された予期せぬ乳児急死事例64例の実態を調査した。その結果、当該事例には第2子以上の男児が多く含まれていた。ほとんどの死亡は生後6ヵ月以内に発生し、多くの児は自宅において深夜の睡眠中に死亡していた。また、呼吸器感染症による急死例が少なからず含まれており、臨床診断がなされていない先天心奇形も認められた。予期せぬ乳児急死予防に向けては、ごく軽度の風邪様症状等に対しても、児への気配りが重要であると思われた。(著者抄録)

    researchmap

  • Child deaths with persistent neglected experiences from medico-legal documents in Japan. Reviewed International journal

    Yui Yamaoka, Nanako Tamiya, Takeo Fujiwara, Yukie Yamasaki, Akemi Matsuzawa, Satoru Miyaishi

    Pediatrics international : official journal of the Japan Pediatric Society   57 ( 3 )   373 - 80   2015.6

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    BACKGROUND: Few studies have examined the actual conditions of fatal neglect in Japan. The aims of this study were to investigate persistent neglect among child fatalities using medico-legal documents, and to describe the characteristics of the socio-familial background and biological data. METHODS: The current study analyzed the documents of all postmortem external examinations and autopsies in children <2 years old carried out in one prefecture in Japan from 2006 to 2011. After examining 59 autopsy cases using modified the Maltreatment Classification System, we identified six children who experienced persistent neglect as study samples. RESULTS: Three children were found in unsanitary rooms and one was left alone inside a car. In two cases, age of mother at delivery was <21 years old. Three victims had 1 year older sibling. With regard to history of use of health services, three mothers missed some prenatal care visits, and two refused to receive neonatal home visits. With regard to biological data the average weight Z score of six children was -2.22 after being adjusted to average weight for age (in months) and sex. Three children had acute or chronic undernutrition. Three victims had thymic involution considered as being due to chronic child maltreatment. CONCLUSIONS: The present collaboration between public health and legal medicine has enabled investigation of the background and biological impact of experiences of persistent neglect. A multidisciplinary system of evaluating child death is needed to identify preventable factors in order to intervene in the case of neglected children in a timely manner.

    DOI: 10.1111/ped.12531

    Web of Science

    PubMed

    researchmap

  • Hydrogen sulfide Reviewed

    28 ( 4 )   346 - 349   2015

  • Helium gas Reviewed

    28 ( 4 )   355 - 358   2015

  • „Verschwinden“ desEpiduralhämatoms im Verlauf. Reviewed

    Miyaishi S, Miura M, Taniguchi K, Püschel K

    Rechtsmedizin   2014

     More details

    Authorship:Lead author, Corresponding author   Language:German   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00194-014-0973-2

    researchmap

  • 同時期に死亡し異なる時期に発見された瀬戸内海漂流死体の3例 Reviewed

    三浦雅布, 宮石智

    法医学の実際と研究   56 ( 56 )   157 - 162   2013

     More details

    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:法医学談話会  

    3例の剖検を担当した。2例目と3例目はそれぞれ死後約1ヵ月と約1ヵ月半後に発見されたが、外表の軟部組織消失程度は2例目のほうが強かった。しかしながら、解剖により内景を観察すると、脳の軟化程度などは3例目のほうが死後変化の進んだ状態であり、組織学的検査においても3例目のほうが細胞融解の程度が強かった。したがって、最終的には2例目と3例目それぞれの死後経過時間に矛盾はないと結論づけることができた。漂流死体の死後変化は、海底に沈んでいた時間や海岸に漂着していた時間などの違いによって異なるため、外表所見のみから死後経過時間を推定することは難しく、内景所見や組織所見などを合わせて総合的に判断することが重要と考えられた。

    researchmap

  • Factors that prolong the 'postmortem interval until finding' (PMI-f) among community-dwelling elderly individuals in Japan: analysis of registration data. Reviewed International journal

    Tomoko Ito, Nanako Tamiya, Hideto Takahashi, Kentaro Yamazaki, Hideki Yamamoto, Shoji Sakano, Masayo Kashiwagi, Satoru Miyaishi

    BMJ open   2 ( 5 )   2012

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:5  

    OBJECTIVES: To clarify the factors affecting 'postmortem interval until finding' (PMI-f) among elderly unexpected death cases. DESIGN: Cross-sectional study. SETTING: All area of Yamagata prefecture in Japan. PARTICIPANTS: Entering subjects were 5675 elderly cases with age of ≥65 years selected from all 9002 cases of unexpected death from 2002 to 2007 in Yamagata prefecture between 2002 and 2007. Our final study subjects consisted of 3387 cases sampled with several criteria to assess the factors to prolong PMI-f. PRIMARY OUTCOME MEASURES: The outcome was the postmortem interval until finding (PMI-f) as the time from death until finding the body which we defined in this study. RESULTS: 'Living alone' showed the highest adjusted HR (3.73, 95% CI 3.37 to 4.13), also 'unnatural death' (1.50, 1.28 to 1.75), 'found at own home' (1.37, 1.22 to 1.55) and 'younger subjects' (0.99, 0.98 to 0.99). In the model including interactions with the household situation, we found 'male subjects living alone' and 'female subjects living with family' tended to be found later. CONCLUSIONS: PMI-f is an effective outcome for quantitative analyses of risk of bodies left. To prevent the elderly dead bodies left for long time, it is necessary to keep regular home-based contact with elderly individuals living alone.

    DOI: 10.1136/bmjopen-2012-001280

    Web of Science

    PubMed

    researchmap

  • Quantitative analysis of DNA degradation in the dead body. Reviewed

    Miki Itani, Yuji Yamamoto, Yusuke Doi, Satoru Miyaishi

    Acta medica Okayama   65 ( 5 )   299 - 306   2011.10

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    Postmortem degradation of DNA was quantitatively estimated. Brain, liver, kidney and muscle samples were obtained from sacrificed rats left at 20 degrees C or 4 degrees C. The quantity of DNA was measured by real-time PCR using a primer set for a sequence in the Rsrc 1 gene. When the quantity of amplified DNA using 10ng Human Genomic DNA was defined as 100 RFU, the quantities in the brain, liver, kidney and skeletal muscle (each 2 mu g of dry weight) on the day of sacrifice were 253 +/- 11, 338 +/- 22, 556 +/- 14 and 531 +/- 12 Relative Fluorescence Units (RFU), respectively (mean +/- S.E., n = 5). The quantity of amplified DNA decreased to below 10 RFU in 1-3 weeks in the liver, kidney and skeletal muscle at 20 degrees C, while that in the brain was more than 10 RFU for six weeks, demonstrating the usefulness of the brain as a sample for DNA analysis of decaying corpses. It was suggested that quantifying the amplified DNA in the brain at 20 degrees C and in the liver at 4 degrees C as well as the ratio of the quantity of amplified DNA in the liver to the brain at 4 degrees C might be useful for diagnosing time of death. This study provides the first quantitative analysis of the postmortem progress of DNA degradation in the corpse.

    DOI: 10.18926/AMO/47011

    Web of Science

    PubMed

    researchmap

  • Postmortem changes in myoglobin content in organs.

    Masanobu Miura, Toru Naka, Satoru Miyaishi

    Acta medica Okayama   65 ( 4 )   225 - 30   2011.8

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    Postmortem changes in myoglobin concentrations in blood and organs were investigated using an enzyme immunoassay by animal experiments in combination with immunohistochemical staining of human cases. Blood myoglobin concentrations were found to increase drastically within a very short time after death. Those in striated muscle, however, did not change by day 14 postmortem. Myoglobin content in the liver and kidney increased slightly by day 5 postmortem, and more obviously by day 7 or later. However, almost no change was observed by day 5 in the kidney when the renal artery and vein had been ligated just after death. In the thyroid gland and the lung, the myoglobin content markedly increased by day 7 postmortem, with the logarithmical values rising nearly linearly as the time after death passed. In the thyroid gland, concentrations reached the level of the striated muscle. The mechanisms of postmortem myoglobin increase in organs are thought to be direct diffusion from the striated muscle and/or distribution through the blood. To estimate the postmortem interval, the determination of myoglobin content in the thyroid gland or the lung appears to be useful.

    DOI: 10.18926/AMO/46847

    Web of Science

    PubMed

    researchmap

  • A new method for sex determination based on detection of SRY, STS and amelogenin gene regions with simultaneous amplification of their homologous sequences by a multiplex PCR. Reviewed

    Toshio Morikawa, Yuji Yamamoto, Satoru Miyaishi

    Acta medica Okayama   65 ( 2 )   113 - 22   2011.4

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male : female ratio as low as 1: 10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.

    DOI: 10.18926/AMO/45270

    Web of Science

    PubMed

    researchmap

  • Water-restraint stress enhances methamphetamine-induced cardiotoxicity. Reviewed International journal

    Masafumi Tomita, Hironobu Katsuyama, Yoko Watanabe, Kazuo Hidaka, Kei Yoshitome, Satoru Miyaishi, Takaki Ishikawa, Kotaro Shinone, Masayuki Nata

    Chemico-biological interactions   190 ( 1 )   54 - 61   2011.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    Methamphetamine (MAP) and stress both cause a variety of cardiovascular problems. Stress also increases stimulant drug-seeking or drug-taking behavior by both humans and animals. In addition to the physiological effects on circulation, metabolism, and excretion, stress affects subject's responses to stimulant drugs such as MAP. However, the mechanisms underlying the drug-stress interactions remain unknown. In the present study, we assessed the effects of stress on myocardial responses to MAP in mice. Mice were injected with MAP (30 mg/kg) immediately before exposure to water-restraint stress (WRS), which has often been used as a stressor in animal experiments. The combination of MAP with WRS produced a significant increase (p &lt; 0.01) in the leakage of proteins specific to myocardial damage and the levels of cytokines IL-6, TNF-alpha, and IL-10. The histological findings indicated the possibility that a combination of MAP with WRS induced cardiac myocytolysis. We also examined the expression of heat shock proteins (Hsps), which have cardioprotective effects. Administration of MAP alone significantly stimulated the RNA expressions of Hsp32, 60, 70, and 90 and the protein Hsp70 in cardiac muscles, whereas the expressions due to WRS or MAP plus WRS were not increased. These results reveal the fact that exposure to WRS depresses the induction of Hsps, in particular Hsp70, due to MAP injection, following to enhance MAP-induced myocardial damage. We believe that interactions between MAP and severe stress, including environmental temperature, affect the induction of Hsps, following to susceptibility of hosts to cardiotoxicity due to the stimulant drug. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.cbi.2011.01.025

    Web of Science

    PubMed

    researchmap

  • ヘルスサービスリサーチ(13)学際的視点から—法医学とHSR—. Reviewed

    伊藤智子, 田宮菜奈子, 宮石智

    日本公衛誌   58 ( 8 )   634 - 639   2011

     More details

    Authorship:Last author   Language:Japanese   Publisher:日本公衆衛生学会  

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/2012011317

  • Postmortem Acidification of Blood/Organs Induces an Increase in Flecainide Concentration in Cardiac Blood and the Contribution of the Lungs to This Increase Reviewed

    Kei Yoshitome, Hideo Ishizu, Satoru Miyaishi

    JOURNAL OF ANALYTICAL TOXICOLOGY   34 ( 1 )   26 - 31   2010.1

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:PRESTON PUBL INC  

    DOI: 10.1093/jat/34.1.26

    Web of Science

    researchmap

  • 溺死診断のための呼気圧出試験の意義 Reviewed

    三浦雅布, 井澗美希, 石川隆紀, 宮石 智

    法医病理   16 ( 2 )   116 - 120   2010

     More details

    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本法医病理学会  

    死体における人為的な呼気圧出音聴取の可・不可と溺死との関連に着眼し、これを知るための外表検査を考案して呼気圧出試験と命名し、その溺死診断上の有用性について検討した。死後経過時間が3日(72時間)以内の法医解剖例113例を対象として呼気圧出試験を実施した。溺死46例では、陽性は2例にとどまり、44例(95.7%)は陰性であった。非溺死67例では陽性が48例、陰性が19例となった。溺死例と非溺死例と比較し、前者と後者との間で統計的有意差があった。溺死診断の基準を満たさず、溺死以外の死因となりうる所見を認めた、非溺死水中死体2例は、何れも呼気圧出試験は陽性であった。陽性の場合には、水中死体であっても溺死でないことを強く疑うべきであり、水中死体で本試験陰性の場合には、その結果をもって溺死と判断はできないが、捜査状況なども含めて総合的に判断する上では、溺死を支持する根拠にはなると考えられた。

    researchmap

  • The medicolegal aspect of hydrogen sulfide poisoning Reviewed

    The Japanese journal of clinical toxicology   23 ( 3 )   217 - 221   2010

  • ガソリン吸入による中毒死の一例 Reviewed

    吉留敬, 三浦雅布, 江口香, 宮石智

    産業医学ジャーナル   33 ( 6 )   36 - 39   2010

     More details

    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:(公財)産業医学振興財団  

    45歳男。荷あげ後の小型タンカーのポンプ室内で蓋の空いた漉し器前の床で意識を失っており、発見後約3時間半に心肺停止状態で救急搬送されたが蘇生しなかった。換気条件下の現場の可燃性ガス濃度は75%LEL、ガソリン濃度は約90%LELであった。剖検所見より頭部、四肢に軽微な皮下出血の散在、眼瞼結膜に多数の溢血点、血液や臓器組織から油臭を認め、肝臓および左睾丸に腫瘤を認めたが病理所見より腫瘤はいずれも良性であった。中毒学的検査より大脳、右心血のトルエン濃度よりガソリン中毒死が示唆され、着衣にガソリンの付着がないことより経皮吸収でなく、高濃度ガソリンによる曝露と判断した。

    researchmap

  • The social affecting factors to the postmortem interval: a study on the situations around death analyzed by Cox’s proportional hazard regression using the record of unexpected deaths for six years in Yamagata, Japan Reviewed

    Ito, T, Tamiya, N, Yamazaki, K, Takahashi, H, Matsuzawa, A, Yamamoto, H, Motozawa, M, Miyaishi, S

    The 88th Jahrestagung der Deutschen Gesellschaft f&uuml;r Rechtsmedizin   2009.9

     More details

    Authorship:Last author   Language:English   Publishing type:Research paper (international conference proceedings)  

    researchmap

  • Factors affecting the choice of suicide method in Okayama: a database analysis from a forensic perspective. Reviewed

    Eigo Kamizato, Kei Yoshitome, Yuji Yamamoto, Toshihide Iwase, Toshihide Tsuda, Satoru Miyaishi, Hiroyuki Doi

    Acta medica Okayama   63 ( 4 )   177 - 86   2009.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    The annual number of suicides in Japan increased sharply in 1998, and since that time it has consistently exceeded 30,000 per year. In this study, we analyze a database of personal and background characteristics of 824 cases (605 men, 219 women) who completed suicide in Okayama Prefecture in 2002 and 2003. The data were obtained with cooperation from the police. Using the methodologies in a previous European study as a model, we classified the suicide methods into 8 categories. To examine the generational and regional differences in the choice of methods, we stratified the sample into 4 age groups (&lt;= 24, 25 similar to 44, 45 similar to 64, and &gt;= 65) and 2 regional groups (Okayama/Kurashiki vs. other areas). Our results on gender differences in 7 of the suicide methods were mostly similar to the European data. However, our data showed a remarkably higher proportionate male-to-female mortality ratio for poisoning by other substances (ICD-10, X65-X69 codes) (1.83, 1.15-2.92). In terms of generational differences in the choice of suicide methods, the Mantel-Haenszel test of homogeneity was significant for most of the categories in our study, suggesting an impact of age on how people commit suicide. There were no remarkable regional differences in our sample. An epidemic curve for suicides via carbon monoxide poisoning using charcoal briquets revealed a trend of time clustering not observed in the other 6 means. The database constructed and used in this study contains richer information than conventional death statistics and is expected to provide helpful knowledge and insights for future epidemiological studies.

    DOI: 10.18926/AMO/31815

    Web of Science

    PubMed

    researchmap

  • A new sex determination method based on simultaneous detection of SRY, STS and Amelogenin gene region and its homologous sequence on X and 7 chromosome Reviewed

    MORIKAWA Toshio, YAMAMOTO Yuji, ITANI Miki, MIYAISHI Satoru

    17   18 - 21   2009

     More details

    Authorship:Last author   Language:Japanese  

    CiNii Article

    CiNii Books

    researchmap

  • 法医剖検例からみた高齢者死亡の実態と背景要因-いわゆる孤独死対策のために-. Reviewed

    松澤明美, 田宮菜奈子, 山本秀樹, 山崎健太郎, 本澤巳代子, 宮石智

    厚生の指標   56 ( 2 )   1 - 7   2009

     More details

    Authorship:Last author   Language:Japanese   Publisher:(一財)厚生労働統計協会  

    目的 高齢者のいわゆる「孤独死」は深刻な社会問題であり,その予防は重要な政策課題である。しかし実証的データはほとんどなく,その定義も議論上にある。そこで本研究は高齢者のいわゆる孤独死対策に向けた基礎的資料を得るために,まず法医剖検例となった高齢者すべての死亡(すなわち誰にも看取られなかった高齢者死亡)の実態と背景要因を明らかにすることを目的とした。特に,これまで狭義の孤独死の定義として議論になってきた点である世帯構成による実態の違いに着目した。方法 岡山大学における平成17〜18年の同一医師による法医剖検例から65歳以上の死者を抽出し,剖検記録から死因の背景要因となる情報を収集した。全体の状況の記述に加え,世帯構成別に分け,背景要因を比較した。結果 剖検例210例のうち65歳以上の61例を分析した結果,死因の種類では「不慮の外因死」が77%,直接死因では「焼死」が全体の41%であった。世帯構成では「独居」46%,対象者の特性では杖歩行や義足,片麻痺,寝たきり等,日常生活動作の自立度が低い事例が36%みられた。発見時の状況では,第一発見者は「近隣の人」が41%で,死亡から発見までの時間では,「1日以上」発見されなかった事例は31%であった。また,「1ヵ月以上」発見されなかった5例のうち,世帯構成の明らかになった3例はすべて「独居」であり,ミイラ化や高度腐乱状態で発見された事例も含まれていた。また,火災等に関する死亡が53%あり,出火原因および場所では「台所・コンロ等」19%,次いで「タバコ」「ストーブ」各16%,「灯明」13%であった。結論 法医剖検例からみた高齢者の看取られない死(このうちすべてが予防すべき孤独であるかは議論を要する)は,世帯構成でみると,約半数が独居であった。また,独居事例では病死が多く,死亡から発見までの時間が長い事例もみられた。これらのことから,高齢者の看取られない死は必ずしも独居者のみの問題ではなく,その対策としては,独居に限らない高齢者への包括的対策,独居者に対しては心理的・社会的孤立予防への対策がより重要と考えられた。また,対象者は約7割が不慮の外因死であり,特に火災等に関する死亡が多くを占めていたことから,高齢者の不慮の事故対策は重要であり,中でも火災への予防的対策は急務の課題であることが明らかになった。高齢者の「孤独死」とは何かについては今後,より議論を要するが,具体的な対策を講じていくため,把握が難しい個々の事例のさらなる実態把握とそれに基づく検討が必要である。そのためには法医剖検例に基づく背景の疫学的検討は非常に有効であり,法医公衆衛生学ともいうべき新たな研究分野が必要と考える。(著者抄録)

    researchmap

    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2009&ichushi_jid=J00462&link_issn=&doc_id=20090216050001&doc_link_id=0900833&url=https%3A%2F%2Fkango-sakuin.nurse.or.jp%2Fnid%2F0900833&type=%8D%C5%90V%8A%C5%8C%EC%8D%F5%88%F8Web&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00014_1.gif

  • 独居生活者および死後長時間経過事例にみる高齢者孤独死の疫学的考察と山形県・東京都区部の地域差 Reviewed

    山崎健太郎, 田宮菜奈子, 松澤明美, 伊藤智子, 宮石智, 梅津和夫, 金涌佳雅, 福永龍繁

    法医学の実際と研究   52 ( 52 )   227 - 235   2009

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:法医学談話会  

    2003〜2007年に山形県警と東京都監察医務院が扱った異状死体のうち、65歳以上の独居生活者、および死亡後発見まで長時間経過(山形県では死後3日以上経過、東京都では4日以上経過)した事例の検案データをもとに地域差の分析を行い、以下の結果を得た。1)65歳以上の全異状死体に占める独居者の割合は東京都区部(以下;東京都)が山形県よりも有意に高かった(36% vs 14%)。2)65歳以上の全異状死体に占める死後長時間経過例の割合も東京都が有意に高かった(13% vs 6%)。3)死後長時間経過例の死因として自殺が占める割合は山形県のほうが有意に高かった(9% vs 3%)。4)死後長時間経過例の発見者は両地域とも「家人・親戚」の割合が最も高かったが、東京都では「隣人・管理人」の割合も高く、「家人・親戚」とほぼ同率であった。5)東京都では「家人・親戚」に発見された割合に男女差がみられ、女性屍のほうが有意に高かった。6)山形県における死後長時間経過例のデータを死後短時間(3日未満)例と群間比較すると、独居生活者の割合が有意に高く、「家人・親戚」に発見された割合が有意に低かった。

    researchmap

  • Postmortem increase of flecainide level in cardiac blood Reviewed

    Kei Yoshitome, Satoru Miyaishi, Yuji Yamamoto, Hideo Ishizu

    JOURNAL OF ANALYTICAL TOXICOLOGY   32 ( 6 )   451 - 453   2008.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PRESTON PUBL INC  

    DOI: 10.1093/jat/32.6.451

    Web of Science

    researchmap

  • A new method for sex determination based on simultaneous detection of SRY gene region and its homologous sequence on X chromosome Reviewed

    MORIKAWA Toshio, YAMAMOTO Yuji, ITANI Miki, MIYAISHI Satoru

    16   226 - 229   2008

     More details

    Authorship:Last author   Language:Japanese  

    CiNii Article

    CiNii Books

    researchmap

  • Immunoassays in forensic biology Reviewed

    Miyaishi Satoru

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   120 ( 1 )   43 - 48   2008

  • 異状死体における糖尿病の罹患状況. Reviewed

    高田智世, 伊藤真帆, 三浦雅布, 井澗美希, 難波令匡, 吉留敬, 山本雄二, 宮石智

    日本医事新報   4385 ( 4385 )   75 - 77   2008

     More details

    Authorship:Last author   Language:Japanese   Publisher:(株)日本医事新報社  

    異常死体における糖尿病の罹患状況を検討した。2002〜2006年の110例(剖検:55例、検死:55例)を対象とした。対象の60.0%(66名)は50〜70歳代、男性が67.3%(74名)であった。「糖尿病」の病歴は「あり」38例(34.5%)、「なし」38例、「不明」34例であったが、HbA1c値から、糖尿病・糖尿病疑い症例は「糖尿病なし」群の34.2%(13例)、「不明」群の32.4%(11例)にみられた。死因は、糖尿病「あり」群は「なし」群よりも有意に内因死が多かった。また、いずれの群においても、内因死は外因死よりもHbA1c値が高かった。異常死体における内因死には糖尿病と気付かれずに未治療であった者や糖尿病の状態が良くない者の割合が多いことが考えられ、異常死減少は糖尿病対策、特に高齢者に対する糖尿病対策ではないかと思われた。

    researchmap

    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2008&ichushi_jid=J01024&link_issn=&doc_id=20080514030008&doc_link_id=%2Faf9mdcla%2F2008%2F004385%2F004%2F0075-0077%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Faf9mdcla%2F2008%2F004385%2F004%2F0075-0077%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • Increase in clusterin-containing follicles in the adenohypophysis of drug abusers. Reviewed International journal

    Takaki Ishikawa, Bao-Li Zhu, Satoru Miyaishi, Hideo Ishizu, Hitoshi Maeda

    International journal of legal medicine   121 ( 5 )   395 - 402   2007.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    The hypothalamic-pituitary-adrenocortical (HPA) system in drug abusers may be affected due to disorders of the hypothalamic dopaminergic system. The present study investigated alterations in the adenohypophysis of middle-aged drug abusers (40-60 years of age), using clusterin-containing mixed cell-follicles as the indicator, in which clusterin (apolipoprotein J) is a multifunctional glycoprotein related to neurodegeneration. The paraffin-embedded adenohypophyses of methamphetamine and psychotropic drug abusers (n=76) were compared with those of non-abusers (n=82). The number of follicles was larger in drug abusers independent of the immediate cause of death, although the size was not significantly different. When cell types forming the follicles were immunohistochemically examined, drug abusers showed an increase of prolactin (PRL) cells and gonadotroph cells and a reciprocal decrease of growth hormone cells, suggesting hypofunction of dopaminergic neurons in the hypothalamus, while there was no change in the adrenocorticotropic hormone and thyroid-stimulating hormone cells. These increases of the clusterin-containing follicles and PRL cells in the follicles may be related to the dysfiunction of dopaminergic neurons in the hypothalamus of chronic drug abusers and may be useful for investigating drug abuse in forensic casework.

    DOI: 10.1007/s00414-006-0138-2

    Web of Science

    PubMed

    researchmap

  • Comparison of German and Japanese general practitioners' awareness of suicide and attitudes toward patients with suicidal ideation Reviewed

    Oliver Redsch, Satoru Miyaishi, Axel Heinemann, Georg Fiedler, Klaus Puschel, Hideki Yamamoto, Hideo Ishizu

    ACTA MEDICA OKAYAMA   60 ( 3 )   159 - 165   2006.6

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    The authors designed a questionnaire to investigate the differences in German and Japanese general Practitioners' (GP) awareness of suicide and attitudes toward patients with suicidal ideation in their respective societies. The purpose of this study was to obtain insights leading to a better means of suicide prevention in primary care in Japan. The background for conducting the study was declining suicides in the past 20 years and the lower suicide rate in Germany compared with the present situation in Japan, where the number of suicides has in recent years continued to exceed 30,000, resulting in a suicide rate approximately 2 times higher than that in Germany. The questionnaire was randomly mailed to GPs in Okayama-Prefecture (western Japan) and Hamburg-State (northern Germany) and was collected in the same way. The patterns of answers were compared between the 2 countries, and the differences were statistically analyzed. Japanese GPs seem to have a lower will to prevent suicide in daily practice compared to German GPs and a great lack of knowledge about treatment of suicidal patients. These observations suggest that improving GPs' interest in the problem of suicide and providing training programs for the treatment of patients with suicidal intentions might be a means of achieving better suicide prevention in Japan.

    DOI: 10.18926/AMO/30747

    Web of Science

    PubMed

    researchmap

  • Distribution of orally ingested hydrochloric acid in the thoracoabdominal cavity after death. Reviewed International journal

    Kei Yoshitome, Satoru Miyaishi, Takaki Ishikawa, Yuji Yamamoto, Hideo Ishizu

    Journal of analytical toxicology   30 ( 4 )   278 - 80   2006.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PRESTON PUBLICATIONS INC  

    The authors encountered a case of hydrochloric acid (HCl) poisoning, thought to be caused by oral ingestion of concentrated HCl. Coagulation of the surface of the tongue and the mucosa of the pharynx, esophagus, and stomach were observed at forensic autopsy. An overabundance of Cl- was found in the gastric contents, corresponding to 8.19 mL of concentrated HCl. This was suggested to be a lethal oral dose of concentrated HCl, and the cause of death was determined to be HCl poisoning. Measuring the pH and concentrations of various ions in body fluids and contents of the alimentary tract enabled postmortem diffusion of HCl to be determined.

    Web of Science

    PubMed

    researchmap

  • Rechtsmedizin in Japan und in Deutschland - Reger wissenschaftlicher Austausch. Reviewed

    Püschel K, Miyaishi S

    Zeitschrift für Japanisches Recht   2006

     More details

    Authorship:Last author   Language:German  

    researchmap

  • 医師法第21条をどう考えるか

    宮石 智

    日本警察医会雑誌   2 ( 2 )   137 - 139   2006

     More details

    Authorship:Lead author   Language:Japanese   Publisher:日本警察医会  

    CiNii Article

    researchmap

  • 溺死診断と呼気圧出試験

    宮石 智

    日本警察医会雑誌   2 ( 2 )   42 - 50   2006

     More details

    Authorship:Lead author   Language:Japanese   Publisher:日本警察医会  

    CiNii Article

    researchmap

  • 開業医の自殺問題への意識ならびに自殺企図者への対応に関する日独比較研究 Reviewed

    Oliver Redsch, 宮石智,Fiedler, Heinemann A, P?schel K, 山本秀樹, 石津日出雄

    自殺予防と危機介   2006

     More details

    Authorship:Corresponding author  

    researchmap

  • A new HLA-DRB1 genotyping method using single nucleotide polymorphism (SNP) analysis with multiplex primer extension reactions and its application to mixed samples. Reviewed

    Kiyomi Imabayashi, Yuji Yamamoto, Sachiyo Inagaki, Yusuke Doi, Kei Yositome, Satoru Miyaishi, Hideo Ishizu

    Acta medica Okayama   59 ( 5 )   179 - 94   2005.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.

    DOI: 10.18926/AMO/31971

    Web of Science

    PubMed

    researchmap

  • Histopathological changes of the hypophysis in malnutrition in elderly subjects. Reviewed

    Takaki Ishikawa, Bao-Li Zhu, Satoru Miyaishi, Hideo Ishizu, Hitoshi Maeda

    Human cell   18 ( 3 )   157 - 62   2005.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Major objectives in forensic gerontology are physical and mental disorders during aging, which can be caused by various factors involving nutrition and stress, often accompanied by dysfunction in the neuroendocrine systems including the hypophysis. The objective of the present study was to investigate the histopathological changes in the adenohypophysis in elderly subjects using autopsy materials. Hypophyses with a scaphoid shape (group S: 16 males and 4 females; mean age, 78.6 years) and a normal one (group C: 30 males and 20 females; mean age, 65.2 years) were compared. Incidence of the scaphoid-shaped hypophysis mildly increased with age, being 17% in the elderly over 65 years of age. The weight of the pituitary gland in group S (0.42 +/- 0.1 g) was lower than that of group C (0.65 +/- 0.2 g). The degree of fibrosis was higher in group S (31.6% +/- 5.4%) than in group C (18.3% +/- 6.3%). Immunohistochemical staining showed no significant differences in the proportion of the ACTH cells and the TSH cells between the two groups (p > 0.05). However, there was an increase in the proportion of gonadotrophs, prolactin cells, and S-100-containing cells in group S and a decrease in that of GH cells (p < 0.05). These findings may be associated with reduced anabolic, gonadal and hepatic functions due to malnutrition.

    DOI: 10.1111/j.1749-0774.2005.tb00006.x

    PubMed

    researchmap

  • A case of sibship test using a corneal tissue of a deceased woman which had been surgically removed and embedded in paraffin while she was alive Reviewed

    YAMAMOTO Yuji, INAGAKI Sachiyo, IMABAYASHI Kiyomi, YOSHITOME Kei, MIYAISHI Satoru, ISHIZU Hideo

    13   259 - 262   2005

     More details

  • Einstellungsmuster japanischer Hausärzte gegenüber suizidgefährdeten Patienten. Reviewed

    Redsch O, Miyaishi S, Inagaki S, Yoshitome K, Yamamoto Y, Imabayashi K, Heinemann A, Püschel K, Ishizu H

    Rechtsmedizin   2005

     More details

    Authorship:Corresponding author   Language:German  

    DOI: 10.1007/s00194-005-0330-6

    researchmap

  • Quantitative analysis of DNA degradation in myoglobin gene for estimating postmortem interval Reviewed

    INAGAKI Sachiyo, MIYAISHI Satoru, YAMAMOTO Yuji, YOSHITOME Kei, IMABAYASHI Kiyomi, ISHIZU Hideo, TAKATA Tomoyo, DOI Yusuke

    13   185 - 188   2005

     More details

  • A new method for ABO genotyping using a multiplex single-base primer extension reaction and its application to forensic casework samples. Reviewed International journal

    Yusuke Doi, Yuji Yamamoto, Sachiyo Inagaki, Yoshiaki Shigeta, Satoru Miyaishi, Hideo Ishizu

    Legal medicine (Tokyo, Japan)   6 ( 4 )   213 - 23   2004.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We developed a new method for ABO genotyping using a multiplex single-base primer extension reaction. The method allows for the simultaneous detection of six SNP sites in the ABO gene (nt 261, 297, 681, 703, 802, and 803) and the determination of ABO genotypes from their combinations. It enabled ABO genotyping of all samples of peripheral blood DNA extracted from 103 Japanese individuals, and had a highly satisfactory detection sensitivity being capable of genotyping 0.1 ng of genomic DNA. Using this method, we were able to determine ABO genotypes of minute stain samples, heated bloodstains, aged bloodstains and mixed samples. Experiments with samples from 26 animal species and bacterial samples to test the species-specificity of the method showed that genotyping was possible in the chimpanzee and gorilla, but their genotypes were extremely rare in humans. In addition, we applied this method to casework samples, and successfully determined ABO genotypes of bones, teeth, muscles, organs, nails, and semen-contaminated vaginal fluid in which ABO grouping by conventional serological techniques was not possible. This new method enables the sensitive, simultaneous detection of six SNP sites in the ABO gene by two specific reactions, i.e. PCR and a primer extension reaction. Therefore, it holds promise as an effective method of ABO genotyping particularly for forensic samples. © 2004 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.legalmed.2004.05.005

    Scopus

    PubMed

    researchmap

  • A new 39-plex analysis method for SNPs including 15 blood group loci. Reviewed International journal

    Sachiyo Inagaki, Yuji Yamamoto, Yusuke Doi, Tomoyo Takata, Takaki Ishikawa, Kiyomi Imabayashi, Kei Yoshitome, Satoru Miyaishi, Hideo Ishizu

    Forensic science international   144 ( 1 )   45 - 57   2004.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI IRELAND LTD  

    A novel 39-plex typing system for single nucleotide polymorphisms (SNPs) has been developed. This multiplex approach has the advantage of being able to type 38 autosomal SNPs and one sex-discriminating base exchange site on the X and Y chromosomes rapidly and simultaneously. The SNP loci on the autosomes, which we examined, contain 15 loci distributed on blood type genes: three on RhCE, two each on Km and Gc, and one each on Duffy, AcPl, Tf, MN, GPT, EsD, PI, and Kidd genes.
    Thirty-seven genomic DNA fragments containing a total of 38 SNPs and one sex-discriminating site were amplified in one multiplex PCR reaction. Following the reaction, single nucleotide primer extension reaction was performed by dividing these SNP loci into five groups. The SNP type of each of the 39 loci was determined at one time by capillary electrophoresis using the newly designed multi-injection method.
    The combined PD (power of discrimination) of this typing system was (1-1.1) X 10(-14), and the MEC (mean exclusion chance) was 0.9990. We applied this system to forensic cases, including 16 paternity testing cases (13 non-exclusion and three exclusion cases) and one personal identification case. For the paternity testing cases, the highest Essen-Moller's W-value was 0.9999995. The pM (matching probability) of the personal identification case was 2.22 x 10(-17). These data showed that this system was an excellent tool for use in forensic cases of paternity testing and personal identification. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.forsciint.2004.03.005

    Web of Science

    PubMed

    researchmap

  • Identification of human brain from a tissue fragment by detection of neurofilament proteins. Reviewed International journal

    Tomoyo Takata, Satoru Miyaishi, Takashi Kitao, Hideo Ishizu

    Forensic science international   144 ( 1 )   1 - 6   2004.8

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI IRELAND LTD  

    We developed a method for identifying human brain from a tissue-like fragment by detection of neurofilament protein (NF) using enzyme-linked immunosorbent assay (ELISA). NF was extracted from 0. 1 g of organ/tissue homogenized with Tris-HCl buffer (pH 7.2) containing urea, phenylmethylsulfonyl fluoride (PMSF), EDTA and, EGTA. It was necessary to dilute the extract at more than 2(3)-fold to avoid immunosuppression by urea. Positive reaction was always obtained for NF-H in 2(3)-fold diluted extract of brain tissue, however, NF-L and NF-M were not always detected when a brain fragment contained gray matter. Human cerebral white matter could be easily distinguished from other organs/tissues by detecting any of the NF-subunits. Brains of human and some animals could be discriminated by detecting NF-L or NF-M, although the species specificity of NF-H was not Good. Our findings suggested that detection of NF-H was more useful than NF-L and NF-M for identifying a brain from a tissue-like fragment. The present ELISA method for NF-H could identify human brain specimens under the following conditions: putrefied at 4 degreesC for up to 3 weeks, dried at 37 degreesC for at least 4 months, heated at 50 degreesC for at least 4 weeks. Our results showed that our method is useful for identification of brain tissue in forensic stain analysis. Two practical cases are described. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.forsciint.2004.01.020

    Web of Science

    PubMed

    researchmap

  • Fatal hypothermia related vacuolation of hormone-producing cells in the anterior pituitary. Reviewed International journal

    T Ishikawa, S Miyaishi, T Tachibana, H Ishizu, B-L Zhu, H Maeda

    Legal medicine (Tokyo, Japan)   6 ( 3 )   157 - 63   2004.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We found small cytoplasmic vacuoles in the hormone-producing cells of anterior pituitary in hypothermic death. The vacuoles were found in approximately 40% of anterior pituitary cells both in males and females that had died from cold (n=31) while the detection rate was lower than 1% (P&lt
    0.001) in the other causes of death (n=180: fire death, n=25
    fatal injury, n=24
    asphyxia, n=20
    poisoning, n=8
    natural diseases, n=103). The detection rate in hypothermic death was the highest in ACTH cells (about 65%), followed by gonadotrophs (about 43%), and the lowest in TSH cells (about 16%) (P&lt
    0.001). These findings suggest that the cytoplasmic vacuoles in the anterior pituitary cells may be the most closely related to cold exposure among the above-mentioned cause of death, providing a supplementary evidence for determining the causes of death. © 2004 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.legalmed.2004.05.004

    Scopus

    PubMed

    researchmap

  • Negligent homicide by traumatic asphyxia. Reviewed International journal

    S Miyaishi, K Yoshitome, Y Yamamoto, T Naka, H Ishizu

    International journal of legal medicine   118 ( 2 )   106 - 10   2004.4

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER-VERLAG  

    We presented an unusual case of negligent homicide by thorax compression, which is the expanded concept of traumatic asphyxia. A 58-year-old man was restrained in the prone position by six prison officers. They were ordered by their superiors to continue restraining him for about 15 min and the victim died. At the forensic autopsy, typical findings of thorax compression with intramuscular hemorrhages on the back and multiple fractures of the ribs were observed. No evidence of neck compression/smothering or other fatal issues likely to occur by chest compression was found. The reconstruction of the scene corresponded exactly with the localization of the injuries found in the victim. This is the first case of death by pure thorax compression without other fatal factors during intentional restraint, in which the force causing the chest compression was distinctly determined by the autopsy and reconstruction.

    DOI: 10.1007/s00414-003-0423-2

    Web of Science

    PubMed

    researchmap

  • Forensic application of SNPs analysis of each chromosome with special interest in blood group loci Reviewed

    INAGAKI Sachiyo, YAMAMOTO Yuji, TAKATA Tomoyo, IMABAYASHI Kiyomi, ITO Maho, YOSHITOME Kei, MIYAISHI Satoru, ISHIZU Hideo, DOI Yusuke

    12   180 - 183   2004

     More details

  • HLA-DRB1 genotyping from mixed samples by a multiplexed single nucleotide primer extension reaction Reviewed

    IMABAYASHI Kiyomi, YAMAMOTO Yuji, INAGAKI Sachiyo, YOSHIOME Kei, ITOH Maho, MIYAISHI Satoru, ISHIZU Hideo, DOI Yusuke, TAKATA Tomoyo

    12   184 - 189   2004

     More details

  • Increase of S-100 protein-positive stellate cells in the anterior pituitary of chronic alcoholic patients with fatty liver or fatty cirrhosis. Reviewed

    Takaki Ishikawa, Toshiaki Tachibana, Hiroshi Ishikawa, Satoru Miyaishi, Hideo Ishizu

    Acta medica Okayama   57 ( 2 )   53 - 8   2003.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    Healthy subjects 40 years old were used as controls in a study of stellate cells (S-100 protein-containing cells, or S-100 cells) in subjects with chronic alcoholism and fatty liver or fatty cirrhosis. S-100 cells were sparsely found in the adenohypophysis of control subjects, and these cells sometimes formed small clusters. However, in chronic alcoholics with fatty liver or fatty cirrhosis, the number of stellate cells in the anterior pituitary tended to be 17 times higher than it was in the control group. No increase in the number of S-100 positive cells that constitute the large and small follicles in the intermediate pituitary. The physiological function of the S-100 protein has not yet been identified. The fact that an increase in prolactin-secreting and growth hormone-secreting cells, as well as a decrease in gonadotrophs were observed in the hypophysis of alcoholics suggests that the function of stellate cells may be closely related to these phenomena. Our results also imply that the stellate cells found in the anterior and intermediate pituitary differ in function although they both produce S-100 proteins.

    DOI: 10.18926/AMO/32818

    Web of Science

    PubMed

    researchmap

  • Analysis of short tandem repeat (STR) polymorphisms by the PowerPlex 16 system and capillary electrophoresis: Application to forensic practice Reviewed

    O Okamoto, Y Yamamoto, S Inagaki, K Yoshitome, T Ishikawa, K Imabayashi, S Miyaishi, H Ishizu

    ACTA MEDICA OKAYAMA   57 ( 2 )   59 - 71   2003.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    Allele and genotype frequencies for 15 short tandem repeat (STR) polymorphisms-D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA-in a Japanese population were estimated. No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for any of the systems studied. Between 2 new pentanucleotide STR loci, Penta E and Penta D, for which there is only limited data regarding the allelic distribution in Japanese, the Penta E locus was found to be highly polymorphic and exhibited a tri- or tetra-modal distribution pattern having allelic peaks with 5, 11, 15 and 20 repeats. The distribution was significantly different from that of the other ethnic groups. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity values (H), polymorphism. information content (PIC), power of discrimination (PD), matching probability (pM), power of exclusion (PE), and typical paternity index (PI), were calculated for the loci. These parameters indicated the usefulness of the loci in forensic personal identification and paternity testing among Japanese. The systems Penta E, FGA, D18S51 and D8S1179 were the most informative. This method was successfully applied to forensic personal identification and paternity testing among Japanese, thereby confirming its efficacy for forensic practice.

    DOI: 10.18926/AMO/32819

    Web of Science

    PubMed

    researchmap

  • Role of adenohypophyseal mixed cell-follicles in age estimation. Reviewed

    Takaki Ishikawa, Satoru Miyaishi, Toshiaki Tachibana, Yuji Yamamoto, Hideo Ishizu

    Acta medica Okayama   57 ( 2 )   83 - 9   2003.4

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicles also tended to enlarge with age. Statistical analysis showed that a high correlation existed between age and the number or the size of the mixed cell-follicles formed by various adenohypophyseal cells. In addition, when the proportions of the different cell types that formed the follicles were examined, sex differences were observed with aging for the GH cells, the PRL cells, and the gonadotroph (GTH) cells, while no changes were observed with aging in both men and women for the ACTH cells and TSH cells. These results indicate that the number, size, and ratio of each component cell of follicles in the anterior pituitary are adequately applicable for the purpose of age estimation in routine forensic medicine.

    DOI: 10.18926/AMO/32816

    Web of Science

    PubMed

    researchmap

  • Sudden unexpected death due to rupture of the stomach. Reviewed International journal

    Takaki Ishikawa, Satoru Miyaishi, Yuji Yamamoto, Kei Yoshitome, Sachiyo Inagaki, Hideo Ishizu

    Legal medicine (Tokyo, Japan)   5 ( 1 )   60 - 4   2003.3

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier  

    We report a case of sudden unexpected death due to rupture of the stomach. A 49-year-old man was found dead in a public lavatory. Autopsy findings revealed two rupture wounds measuring 14 cm and 6 cm located in the fundus of stomach at the side of the greater curvature despite of any superficial injury. The deceased had an ulcer in the lesser curvature of stomach, and dilation in this area was expected to be impaired. Under this condition, excessive over-eating resulting in over-extension of the stomach wall at the greater curvature was speculated to have caused stomach rupture. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S1344-6223(02)00034-2

    Scopus

    PubMed

    researchmap

  • ヒト脳下垂体の中葉で観察された単核細胞群(Mononuclear Cell Clusters Observed in Pars Intermedius of Human Hypophysis) Reviewed

    石川 隆紀, 小原 健司, 宮石 智, 石津 日出雄, 加来 浩平

    Kawasaki Medical Journal   29 ( 1・2 )   17 - 24   2003

     More details

    Language:English   Publisher:川崎医学会  

    ヒト脳下垂体の中葉において形態学的にリンパ球に類似した細胞群を観察し,その免疫組織化学的性質を検討した.これらの細胞群は,腺下垂体中に存在するホルモンに対する抗原またはS-100蛋白に対する抗原によって免疫染色されなかったが,抗B細胞膜抗原によって免疫染色された.これらの細胞が中葉でのみ認められる理由,主としてB細胞である理由,その機能については,本研究で明らかにすることはできなかった.この細胞群と脳下垂体中葉の機能の間に相関性は認められなかったが,ヒトの中葉は両生類,鳥類,齧歯類に比べるとより縮退しており,未知の機能を有することが考えられる

    researchmap

  • An autopsy case of death after trauma in an elderly subject with pulmonary squamous cell carcinoma From the viewpoint of forensic medicine in an aging society

    ISHIKAWA Takaki, FURUSATO Masakuni, YOSHITOME Kei, MIYAISHI Satoru, ISHIZU Hideo

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   115 ( 1 )   33 - 37   2003

     More details

    Language:Japanese   Publisher:Okayama Medical Association  

    In this study, we experienced a case of an elderly man with clinically undiagnosed squamous cell carcinoma of the lung, who died 8 days after a trauma. This case was autopsied because of a question over the causal association between the trauma and death. From the autopsy findings, it was judged that respiratory and metabolic abnormalities due to lung cancer were exacerbated by trauma, and that medically the cause of death should be assessed as death by disease.

    DOI: 10.4044/joma1947.115.1_33

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/2004010908

  • Two Cases of Death from Hypothermia Occurring Indoors in Early Spring in Okayama Prefecture

    ISHIKAWA Takaki, MIYAISHI Satoru, DOI Yusuke, TAKATA Tomoyo, IMABAYASHI Kiyomi, INAGAKI Sachiyo, YOSHITOME Kei, YAMAMOTO Yuji, ISIHZU Hideo

    114 ( 3 )   303 - 308   2003

     More details

    Authorship:Corresponding author   Language:Japanese  

    We encountered two cases of unnatural death occurring indoors in early spring in Okayama Prefecture. The two cases were both females aged 31yearsold and 88yearsold. Autopsies revealed death from hypothermia as the cause of death. The diagnosis of death from cold was not based solely on the characteristic findings of the dead body. After confirming that there were no other accidents and diseases that may cause death, an overall evaluation should be made considering the conditions surrounding occurrence of death from cold. We describe these procedures using these two autopsy cases of death from hypothermia.

    DOI: 10.4044/joma1947.114.3_303

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/2003248180

  • SNPs analysis of each chromosome with special interest in blood group loci Reviewed

    INAGAKI Sachiyo, YAMAMOTO Yuji, TAKATA Tomoyo, ISHIKAWA Takaki, IMABAYASHI Kiyomi, YOSHITOME Kei, MIYAISHI Satoru, ISHIZU Hideo, DOI Yusuke

    11   167 - 171   2003

     More details

  • A method for HLA-DRB1 genotyping by SNPs analysis Reviewed

    IMABAYASHI Kiyomi, YAMAMOTO Yuji, INAGAKI Sachiyo, YOSHITOME Kei, ISHIKAWA Takaki, MIYAISHI Satoru, ISHIZU Hideo, DOI Yusuke, TAKATA Tomoyo

    11   157 - 162   2003

     More details

  • 脳下垂体前葉中のS-100蛋白陽性星状細胞を用いた年齢の推定(Age Estimation Using S-100 Protein-Positive Stellate Cells in Anterior Pituitary) Reviewed

    石川 隆紀, 立花 利公, 宮石 智, 石津 日出雄

    Kawasaki Medical Journal   29 ( 1・2 )   25 - 31   2003

     More details

    Language:English   Publisher:川崎医学会  

    ヒト胎児屍体3体および内分泌学的疾患,アルコール関連疾患,肝疾患,精神疾患,悪性腫瘍が無い屍体132体(0〜100歳)から得た脳下垂体切片を用いて,下垂体前葉におけるS-100蛋白陽性星状細胞を免疫組織化学的に検討し,星状細胞数を用いた年齢推定の可能性について検討した.星状細胞の割合と年齢の間には,男女のいずれにおいても明確な正の相関が認められ,それぞれについて線形回帰方程式が得られた.年齢と共に星状細胞が増加する理由は明らかではないが,この結果から脳下垂体前葉の星状細胞の割合を年齢推定に応用できることが示唆された

    researchmap

  • Evaluation of a method for typing the microsatellite D12S391 locus using a new primer pair and capillary electrophoresis. Reviewed

    Yoshiaki Shigeta, Yuji Yamamoto, Yusuke Doi, Satoru Miyaishi, Hideo Ishizu

    Acta medica Okayama   56 ( 5 )   229 - 36   2002.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evaluated using various forensic samples. The typing results from 70 DNA samples using the new primer pair and the original primer pair were completely identical. One hundred twenty-five amplified fragments from D12S391 alleles were sized correctly within +/-0.25 by of the D12S391 allelic ladder. A rare allele, 19.3, previously found only in Caucasians, was found for the first time in a Japanese subject, and it was clearly distinguished from allele 20 by the CE analysis. This detection system was sensitive and could detect D12S391 types from 16 pg of genomic DNA, and from a minor component at a ratio of 1:10 in mixed samples. This system was more useful for the analysis of degraded DNA than was the method using the original primer pair, and could detect D12S391 types from bloodstains that had been stored for 26 years. In addition, the specificity of the method was demonstrated using nonhuman DNA.

    DOI: 10.18926/AMO/31707

    Web of Science

    PubMed

    researchmap

  • Allele frequencies of single nucleotide polymorphisms in the second exon of the myoglobin gene among the Japanese. Reviewed International journal

    Tomoyo Takata, Satoru Miyaishi, Yuji Yamamoto, Sachiyo Inagaki, Kei Yoshitome, Takaki Ishikawa, Hideo Ishizu

    Human biology   74 ( 2 )   317 - 20   2002.4

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WAYNE STATE UNIV PRESS  

    The difference in the allele frequencies of two single nucleotide polymorphisms (SNPs) in the second exon of the myoglobin gene between Japanese and other populations is reported. These SNPs are the substitutions of (A79G) and (T109C), and they were investigated by a single polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis followed by direct sequencing. The substitutions were always linked and two alleles were found in the samples used: the A-T allele with no substitution at positions (79A) and (109T) and the G-C allele with substitutions of (79G) and (109C). The frequencies of these alleles were 0.755 bind 0.245, respectively, and they were found to be in Hardy-Weinberg equilibrium. The distribution of alleles in the Japanese population was significantly different from that reported among whites, blacks, and Hispanics (p &lt; 0.0001).

    Web of Science

    PubMed

    researchmap

  • A case of suffocation by an advertising balloon filled with pure helium gas. Reviewed

    Kei Yoshitome, Takaki Ishikawa, Sachiyo Inagaki, Yuji Yamamoto, Satoru Miyaishi, Hideo Ishizu

    Acta medica Okayama   56 ( 1 )   53 - 5   2002.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.

    DOI: 10.18926/AMO/31726

    Web of Science

    PubMed

    researchmap

  • Typing of Y chromosome single nucleotide polymorphisms (SNPs) in a Japanese population by a multiplexed single nucleotide primer extension reaction Reviewed

    INAGAKI Sachiyo, YAMAMOTO Yuji, YOSHITOME Kei, TAKATA Tomoyo, ISHIKAWA Takaki, IMABAYASHI Kiyomi, MIYAISHI Satoru, ISHIZU Hideo, DOI Yusuke

    10   162 - 172   2002

     More details

  • Forensic application of ABO genotyping by multiplexed SNPs analysis Reviewed

    DOI Yusuke, YAMAMOTO Yuji, INAGAKI Sachiyo, YOSHITOME Kei, TAKATA Tomoyo, ISHIKAWA Takaki, IMABAYASHI Kiyomi, MIYAISHI Satoru, ISHIZU Hideo

    10   229 - 233   2002

     More details

  • Human identification from forensic materials by amplification of a human-specific sequence in the myoglobin gene. Reviewed

    T Ono, S Miyaishi, Y Yamamoto, K Yoshitome, T Ishikawa, H Ishizu

    Acta medica Okayama   55 ( 3 )   175 - 84   2001.6

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degreesC for 6 months, and from bloodstains heated at 150 degreesC for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.

    DOI: 10.18926/AMO/32024

    Web of Science

    PubMed

    researchmap

  • 尿中ミオグロビンの定量におけるpH及び尿素の影響. Reviewed

    北尾孝司, 高田智世, 宮石 智

    愛媛県臨床検査技師会誌   2001

     More details

    Authorship:Corresponding author  

    researchmap

  • A new method for ABO genotyping by SNPs analysis using SNaPshot ddNTP Primer Extension Kit Reviewed

    DOI Yusuke, YAMAMOTO Yuji, INAGAKI Sachiyo, MIYAISHI Satoru, ISHIZU Hideo

    9   254 - 257   2001

     More details

  • Two judicial autopsy cases of sudden unexpected natural death

    ISHIKAWA Takaki, MIYAISHI Satoru, YAMAMOTO Yuji, YOSHITOME Kei, INAGAKI Sachiyo, OKAMURA Michihiko, ISHIZU Hideo

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   113 ( 3 )   241 - 245   2001

     More details

    Authorship:Corresponding author   Language:Japanese   Publisher:Okayama Medical Association  

    Two judicial autopsy cases of sudden unexpected natural death were reported. In both cases it was suspected that the victims had died of a traffic accident or violent injury. It became clear, however, that both the cause of death had been myocardial infarction by the forensic autopsies and that the suspects had not been criminally responsible for the death of the victims. It is expected that sudden unexpected natural death whose cause and manner are unclear at first may occur more frequently following the increase of the number of death from ischemic heart disease in the future. In these circumstances the forensic autopsy of such cases will become more important socially.

    DOI: 10.4044/joma1947.113.3_241

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/2002229381

  • Sudden Death of a 34-Year-Old Driver at the Wheel of a Large Trailer Truck Reviewed

    ISHIKAWA Takaki, MIYAISHI Satoru, ONO Toshiaki, YOSHITOME Kei, YAMAMOTO Yuji, ISHIZU Hideo

    67 ( 1 )   34 - 40   2001

     More details

    Authorship:Corresponding author   Language:Japanese  

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/2001181637

  • Forensic study of sex determination using PCR on teeth samples. Reviewed

    H Murakami, Y Yamamoto, K Yoshitome, T Ono, O Okamoto, Y Shigeta, Y Doi, S Miyaishi, H Ishizu

    Acta medica Okayama   54 ( 1 )   21 - 32   2000.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    In this study, sex determination using polymerase chain reaction (PCR) on tooth material was evaluated from the viewpoint of forensic medicine. The sensitivity of PCR for detection of the Y chromosome-specific alphoid repeat sequence and the X chromosome-specific alphoid repeat sequence was 0.5 pg of genomic DNA. Sex could be determined by PCR of DNA extracted from the pulp of 16 freshly extracted permanent teeth and dentine including the surface of the pulp cavity of 6 freshly extracted milk teeth. Sex could be determined using the pulp in all 20 teeth (10 male and 10 female) preserved at room temperature for 22 years. For the pulp of teeth stored in sea water, the sex could be determined in all 8 teeth immersed for 1 week and in 5 of 6 teeth immersed for 4 weeks. In the remaining 1 tooth, in which sex determination based on the pulp failed, the sex could be determined correctly when DNA extracted from the tooth hard tissue was examined. For teeth stored in soil, the sex could be determined accurately in all 8 teeth buried for 1 week, 7 of 8 teeth buried for 4 weeks, and in all 6 teeth buried for 8 weeks. When teeth were heated for 30 min, sex determination from the pulp was possible in all teeth heated to 100, 150, and 200 degrees C, and even in some teeth heated to 250 degrees C. When this method was applied to actual forensic cases, the sex of a mummified body estimated to have been discovered half a year to 1 year after death could be determined readily by examination of the dental pulp. In the skeletons of 2 bodies placed under water for approximately 1 year and approximately 11 years and 7 months, pulp tissues had been dissolved and lost, but sex determination was possible using DNA extracted from hard dental tissues. These results indicate that this method is useful in forensic practices for sex determination based on teeth samples.

    DOI: 10.18926/AMO/32309

    Web of Science

    PubMed

    researchmap

  • Species identification by PCR of myoglobin gene Reviewed

    ONO Toshiaki, MIYAISHI Satoru, YAMAMOTO Yuji, ISHIZU Hideo

    8   116 - 119   2000

     More details

    Authorship:Corresponding author   Language:Japanese  

    CiNii Article

    CiNii Books

    researchmap

  • Polymorphism of the D12S391 microsatellite in a Japanese population sample. Reviewed International journal

    Y Shigeta, Y Yamamoto, Y Doi, S Miyaishi, H Ishizu

    Forensic science international   102 ( 1 )   61 - 6   1999.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI IRELAND LTD  

    Using the polymerase chain reaction (PCR), we studied the short tandem repeat (STR) polymorphism observed at the D12S391 locus. In 350 Japanese examined, 14 different alleles ranging from 209 bp to 261 bp were detected. Allele 18 (221 bp) showed the highest frequency at 0.30. Observed and expected values of respective genotypes satisfied the Hardy-Weinberg equilibrium (chi(2)=24.08, P=0.24, df=20). In addition, 18 additional sequence structures (suballeles), were detected in this study. Within the suballeles, sequence variants, in which the initial repeat of (AGAT) was replaced with (AGGT), was found in five samples. It was found that the analysis of single strand conformation polymorphism (SSCP) before sequence analysis was useful for distinguishing these suballeles. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0379-0738(99)00048-1

    Web of Science

    PubMed

    researchmap

  • Effects of pH and urea on the determination of urinary myoglobin concentration by an enzyme immunoassay. Reviewed International journal

    T Kitao, S Miyaishi, H Ishizu

    Clinica chimica acta; international journal of clinical chemistry   282 ( 1-2 )   203 - 9   1999.4

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    DOI: 10.1016/S0009-8981(99)00011-X

    Web of Science

    PubMed

    researchmap

  • 塗装作業中における急性トルエン中毒死の1事例-1名死亡、2名生存の労災事故 Reviewed

    吉留敬, 小野俊明, 石川隆紀, 山本雄二, 宮石智, 堀家徳士, 吉良尚平, 石津日出雄

    法医学の実際と研究   ( 42 )   167 - 172   1999

     More details

    Language:Japanese   Publisher:法医学談話会  

    塗装作業現場において作業員3名が倒れているのが発見された.1名は死亡していたが2名は生命をとりとめた.死者(21歳男)の司法解剖では,急死の所見が認められた他には,死因となるような損傷も病変も認められなかった.剖検時採取した試料についてGC/MSによる検査を行った結果,全ての試料からトルエンが検出され,その他の有機溶媒成分は全く検出されなかった.GCによる諸試料のトルエン濃度測定結果は,血液で65.8μg/g(右心血),44.9μg/g(左心血),20.3μg/g(大腿静脈血),脳で437.9μg/g(脳幹),465.4μg/g(大脳)であり,致死レベルに達していたことから死因は急性トルエン中毒と判断された.また死者の膀胱内には410mlの尿が貯留しており,尿中馬尿酸濃度は82.9mg/mlと著しく高値であった.本事例において,死者は著しく高濃度のトルエン蒸気に長時間暴露された後死亡したものと推定された

    researchmap

  • 県道脇の池から発見された多発外傷を伴う水死体の1剖検例. Reviewed

    宮石智, 小野俊明, 吉留敬, 山本雄二, 石川隆紀, 石津日出雄

    法医学の実際と研究   ( 42 )   161 - 165   1999

     More details

    Authorship:Corresponding author   Language:Japanese   Publisher:法医学談話会  

    死者は当初は不審な点が多々あった.死者の足取りは死体発見前日の夕立の頃で途切れていた.解剖で死因は溺死と判明し,損傷は高所からの転落によって形成された可能性が高いと判断されたその後,死者は死体発見前日に夕立の中を家へ急いでいた,雨が止んだ後の路上に所持品が落ちていた,雨水は側溝を鉄砲水の如くに流れていた,死者が歩いていた道路の側溝は死体の発見された池へつながっている,などのことが判明し,側溝から池へ至る水路の途中から死者のバッグが発見された.死亡時刻は,捜査で判明した食事時間と剖検で判明した食後経過時間から,足取りの途絶えた夕立の頃と特定された.死体に見られた全ての損傷は,水路を流される過程で形成されたものとして説明された.以上から,「死者は豪雨の中を歩行中に側溝へ転落,大量の水と共に水路を流され,途中で身体諸処を強打する等しつつ池へ流れ出た」と結論された

    researchmap

  • Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP. Reviewed

    S Inoue, Y Yamamoto, O Okamoto, H Murakami, S Miyaishi, H Ishizu

    Acta medica Okayama   52 ( 6 )   289 - 96   1998.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the typing of HLA-DRB1 alleles. By this method, HLA-DRB1 typing was possible from 10 pg of genomic DNA extracted from lymphocytes and from 0.5 mu l of 1,000 times diluted blood without DNA extraction. HLA-DRB1 alleles could be typed from a 2-mm long bloodstained cotton thread prepared from 10 times diluted blood and from a 2-mm thread of whole blood bloodstains stored at room temperature for 2 years. From the mixture of blood of two individuals with different genotypes, DRB1 alleles of the minor component were detected down to 1/1,000 of the major component. This semi-nested PCR-RFLP method is useful for HLA-DRB1 typing from extremely small amounts of DNA and from mixed samples.

    DOI: 10.18926/AMO/31305

    Web of Science

    PubMed

    researchmap

  • Haptoglobin genotyping by allele-specific polymerase chain reaction amplification. Reviewed

    A Yano, Y Yamamoto, S Miyaishi, H Ishizu

    Acta medica Okayama   52 ( 4 )   173 - 81   1998.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OKAYAMA UNIV MED SCHOOL  

    We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp(2) = 0.723 and Hp(1S) = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 mu l of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp'S, were detected in those heated at 100 degrees C for 2h. In bloodstains, Hp(2) and Hp(1S) were detected in samples heated at 100 degrees C for 2h and 120 degrees C for 30min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.

    Web of Science

    PubMed

    researchmap

  • Body-packing as cause of unexpected sudden death. Reviewed International journal

    A Heinemann, S Miyaishi, S Iwersen, A Schmoldt, K Püschel

    Forensic science international   92 ( 1 )   1 - 10   1998.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI IRELAND LTD  

    Four fatalities related to smuggling of drugs by body-packing were investigated. The victims were examined at the Institute of Forensic Medicine of Hamburg University between 1983 and 1995, two of them due to "sudden" unknown cause of death. All victims were male. Two of them were found already dead in a backyard and in a hotel, two other were emergency cases and died at a hospital. Smuggled substances included cocaine (two cases), heroin and amphetamine/caffeine. In all cases, the cause of death was intoxication caused by tom packages which were detected at autopsy. The maximum weight of the packet's contents was 630 g divided in 90 packages. Only one victim was apparently an intravenous drug-abuser. Hair analysis was performed in three cases and revealed in one case a difference between a concealed and a habitually consumed drug. Toxicological analysis revealed that the substances were quite pure and provided evidence that rather long survival was possible following intoxication in three cases, in two cases supported by hospital treatment in the final stage. The procedural regimen in cases of suspected body-packing is discussed. (C) 1998 Elsevier Science Ireland Ltd.

    DOI: 10.1016/S0379-0738(97)00192-8

    Web of Science

    PubMed

    researchmap

  • 異状死体におけるHBs抗原、抗HCV抗体、及び抗HIV抗体の陽性率. Reviewed

    宮石 智, 山本雄二, 吉留敬, 小野俊明, 岡村倫彦, 石津日出雄

    日本医事新報   3891 ( 3891 )   42 - 44   1998

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:(株)日本医事新報社  

    異状死体にはHBV及びHCVに関して感染性を有する可能性のある例が高率に含まれている.検案現場は血液で広く汚染されている場合がままあり,死体検案における感染予防対策が必要である

    researchmap

  • Analysis of STR Polymorphisms by Geneprint^<TM>Powerplex System and Capillary Electrophoresis Reviewed

    YAMAMOTO Yuji, SHIGETA Yoshiaki, MIYAISHI Satoru, ISHIZU Hideo

    6   188 - 191   1998

     More details

  • Microsatellite polymorphism of D 12 S 391 Locus in a Japanese Population Reviewed

    SHIGETA Yoshiaki, YAMAMOTO Yuji, MIYAISHI Satoru, ISHIZU Hideo

    6   176 - 178   1998

     More details

  • Identification of human skin from tissue fragments left in various conditions using an enzyme immunoassay for squamous cell carcinoma-related antigen. Reviewed

    T Kitao, S Miyaishi, H Ishizu

    Nihon hoigaku zasshi = The Japanese journal of legal medicine   51 ( 4 )   297 - 300   1997.8

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    We investigated the possibility of identification of human skin left under various conditions using our original enzyme immunoassay (EIA) for squamous cell carcinoma-related (SCC) antigen. The antigen could be detected in specimens under the following conditions: putrefied or dried at room temperature for at least 12 months, immersed in fresh water at room temperature for 3 weeks and heated at 100°C for 72 h.

    Scopus

    PubMed

    researchmap

  • Sensitive detection of STR polymorphisms Reviewed

    YAMAMOTO Yuji, INOUE Seiichi, MIYAISHI Satoru, ISHIZU Hideo

    5   44 - 46   1997.7

     More details

  • アルコール濃度の測定法

    中村 智恵美, 宮石 智, 石津 日出雄

    日本臨床   55 ( 特別 )   659 - 664   1997.2

     More details

    Language:Japanese   Publisher:(株)日本臨床社  

    researchmap

  • Identification of human skin from a tissue fragment by detection of squamous cell carcinoma-related antigen using an enzyme immunoassay. Reviewed International journal

    T Kitao, S Miyaishi, Y Yamamoto, H Ishizu

    Forensic science international   83 ( 2 )   81 - 6   1996.12

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI IRELAND LTD  

    A new method of identifying human skin from a tissue fragment by detection of developed. When an extract was prepared from 0.1 g human skin homogenized with 1 ml of phosphate buffered saline, this method was able to detect SCC antigen in extracts diluted 10(2)-fold. There was no difference in the detection limit between individuals. Species specificity was good, and there was no cross reaction observed with skins from animals. Our method could also discriminate between skin and other organs or tissues, except for esophagus and lung. A practical case to which this method was applied is presented.

    DOI: 10.1016/S0379-0738(96)02022-1

    Web of Science

    PubMed

    researchmap

  • ハンブルク大学における法医学教育について Reviewed

    宮石 智

    法医学の実際と研究   ( 39 )   335 - 340   1996.12

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publisher:法医学談話会  

    researchmap

  • Forensic Practice in Hamburg Reviewed

    MIYAISHI Satoru

    50 ( 5 )   366 - 373   1996.10

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:Japanese  

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/1997101638

  • Species Identification by Analysis of Amelogenin Gene Reviewed

    YAMAMOTO Yuji, INOUE Seiichi, MIYAISHI Satoru, ISHIZU Hideo

    4   195 - 198   1996.7

     More details

  • 月経血のFDP-Dダイマーおよびミオグロビン濃度 Reviewed

    宮石 智, 北尾 孝司, 石津 日出雄

    臨床婦人科産科   50 ( 2 )   205 - 206   1996.2

     More details

    Language:Japanese   Publisher:(株)医学書院  

    月経血のFDP-Dダイマーならびにミオグロビン濃度を測定した.FDP-Dダイマー濃度は1.02×105±4.47×104ng/ml(102±44.7μg/ml,mean±SD)で,末梢血中濃度の2,000倍以上の濃度であった.又,FDP-Dダイマー濃度は凝血塊の有無による差はなかった.ミオグロビン濃度は66.3±30.7ng/ml(mean±SD)で,末梢血中濃度と同程度とみなされた

    researchmap

  • Identification of menstrual blood by the simultaneous determination of FDP-D dimer and myoglobin contents Reviewed

    S. Miyaishi, T. Kitao, Y. Yamamoto, H. Ishizu, T. Matsumoto, Y. Mizutani, A. Heinemann, K. Püschel

    Japanese Journal of Legal Medicine   50 ( 6 )   400 - 403   1996

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    FDP-D dimer (D-D) and myoglobin concentrations in peripheral, menstrual and postmortem bloodstains were determined. The mean plasma concentrations of D-D in peripheral, menstrual and postmortem blood were 0.047, 102 and 220 μg/ml and those of myoglobin were 0.028, 0.066 and 727 μg/ml respectively. The mean D-D concentration in menstrual bloodstains was about 200 times higher than in peripheral bloodstains. The myoglobin contents in both bloodstains were similar. The mean myoglobin content in postmortem bloodstains was about 4000 times higher than in menstrual bloodstains. By the simultaneous determination of D-D and myoglobin contents, blood or bloodstains containing large amounts of D-D and only a small amount of myoglobin could be identified as menstrual blood.

    Scopus

    PubMed

    researchmap

  • Schusstodesfaelle im Zusammenhang mit der Jagd sowie durch Jagdwaffen-Ursachen, Verhuetung und Beurteilung Reviewed

    Janssen, et

    Arch Kriminol   197 ( (1-2) )   1 - 15   1996

     More details

    Language:German   Publishing type:Research paper (scientific journal)  

    researchmap

  • Microsatellite Polymorphism at the HPRT Locus on X Chromosome Reviewed

    TAKATA Shingo, YAMAMOTO Yuji, MIYAISHI Satoru, ISHIZU Hideo

    3   121 - 123   1995.7

     More details

  • IDENTIFICATION OF HUMAN SKELETAL-MUSCLE FROM A TISSUE FRAGMENT BY DETECTION OF HUMAN MYOGLOBIN USING A DOUBLE-SANDWICH ELISA Reviewed International journal

    T KITAO, S MIYAISHI, H ISHIZU

    FORENSIC SCIENCE INTERNATIONAL   71 ( 3 )   205 - 214   1995.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI PUBL IRELAND LTD  

    A method for identifying human skeletal muscle by detection of human myoglobin using a double-sandwich ELISA was developed. When an extract was prepared from 0.1 g skeletal muscle homogenized with 10 ml PBS, this method was able to detect human myoglobin in extracts diluted 10(4)-fold. There was no difference in the detection limit between individuals or sites of origin of skeletal muscles. Species specificity was good and no cross reaction occurred with skeletal muscle from other animals except the gorilla. Our method could also discriminate between skeletal muscle and other organs or tissues except the heart. Human myoglobin could be detected in skeletal muscles under the following conditions: putrefied at room temperature for 5 months, dried at room temperature for 11 months, heated at 100 degrees C for 72 h and immersed in fresh water at room temperature for 6 days. Two practical cases to which this method was applied are presented.

    Web of Science

    PubMed

    researchmap

  • 傷害致死が疑われた心サルコイドーシスの1剖検例 Reviewed

    宮石 智, 寺本 典弘, 山本 雄二

    法医学の実際と研究   37   335 - 339   1994.11

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:法医学談話会  

    researchmap

  • MASSIVE PULMONARY EMBOLIZATION WITH CEREBRAL TISSUE DUE TO GUNSHOT WOUND TO THE HEAD Reviewed

    S MIYAISHI, F MORIYA, Y YAMAMOTO, H ISHIZU

    BRAIN INJURY   8 ( 6 )   559 - 564   1994.8

     More details

    Authorship:Lead author, Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Autopsy findings in a 22-year-old man who received a gunshot wound to the head and died at the scene are presented. Massive white-grey emboli were found in the pulmonary arteries, and these were identified as cerebral cortical or glial tissue by histological examination. It was thought that the momentary severe increase of intracranial pressure and simultaneous rupture of the superior sagittal sinus due to the kinetic energy of the bullet had caused the lacerated cerebral tissue to enter the blood stream.

    Web of Science

    researchmap

  • 溶血液からの血液型迅速判定法 Reviewed

    宮石 智, 山本 雄二, 石津 日出雄

    犯罪学雑誌   60 ( 4 )   135 - 141   1994.8

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publisher:日本犯罪学会  

    小さなニトロセルロース膜を溶血液に浸し,大型試験管に入れた。それらの膜を5分間振って抗血清またはレクチンと反応させ,それから5分間洗浄した。55°Cで5分間抗体またはレクチンの溶離後に,該当する標識細胞を加えた。試験管を流水で冷却し,1分間振ってから遠心分離した。血球凝集反応の存在または不在を肉眼で観察した。本法を用いると,たとえ材料がひどく腐敗していたとしても,溶血液のABO血液型は30分以内に正確に同定された

    researchmap

  • Discrimination between postmortem and antemortem blood by a dot-ELISA for human myoglobin Reviewed

    S. Miyaishi, F. Moriya, Y. Yamamoto, T. Kitao, H. Ishizu

    Japanese Journal of Legal Medicine   48 ( 6 )   433 - 438   1994

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publisher:(NPO)日本法医学会  

    A method for discriminating between postmortem and antemortem blood from bloodstains by detection of human myoglobin using a dot-ELISA was devised, and its applicability to forensic practice was investigated. This method exploits the high amount of myoglobin present in postmortem blood in comparison with that in antemortem blood. Our dot-ELISA was able to detect human myoglobin from bloodstains containing more than 10 μg/ml myoglobin, the level commonly observed in postmortem blood. Using this method, 10 stains of postmortem blood and 10 of antemortem blood were all identified correctly. A one-year-old stain made of postmortem blood and a stain of bloody fluid obtained from a severely putrefied body 4 months after death were identified as postomortem blood by this method. Two practical cases for which this method was applied are presented.

    Scopus

    PubMed

    researchmap

    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1994&ichushi_jid=J01194&link_issn=&doc_id=19950040090006&doc_link_id=40003039881&url=https%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F40003039881&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

  • フグ中毒死の2剖検例 Reviewed

    宮石 智

    日本法医学雑誌   47 ( 2 )   187 - 187   1993.4

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • Km Genotyping by polymerase chain Reaction (PCR) Reviewed

    Yamamoto Y

    DNA Polymorphism????   1,   69 - 74   1993

     More details

    Publishing type:Research paper (scientific journal)  

    researchmap

  • 民家火災後に死体で発見された一家6人についての法医学的考察 Reviewed

    宮石智

    法医学の実際と研究   36   115 - 121   1993

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:法医学談話会  

    民家火災の焼け跡からこの家の一家6人全員が死体で発見された。家族構成は主人(36歳),妻(30歳),長男(9歳),次男(7歳),長女(4歳)及び主人の祖母(87歳)である。主人,妻,長男,次男及び長女の5名には,胸部などに多数の刺創を認めた。主人の祖母は焼損が高度で,外表からは創傷の有無や焼死と死後焼却の鑑別は困難であった。当初,部外者による殺人・放火事件が強く疑われ,全員の司法解剖が行われた。主人のみは煙の吸引による一酸化炭素中毒により死亡していたが,他の5名は創傷に基づく失血ないし心臓損傷で死亡していることなどが判明し,その他の剖検所見も併せて,本件は主人の引き起こした無理心中事件と考えられた

    CiNii Article

    researchmap

  • An autopsy case of accidental death from erroneous drip infusion of thrombin

    ISHIZU Hideo, MIYAISHI Satoru, YAMAMOTO Yuji, TAKATA Shingo

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   105 ( 9 )   839 - 845   1993

     More details

    Language:Japanese   Publisher:Okayama Medical Association  

    A 63-year-old male died in the hospital from erroneous drip infusion of thrombin, a local hemostatic, after polypectomy for intestinal polyposis. At autopsy, thromboses were observed in the cutaneous veins of the right arm, the right axillary vein and the right subclavian vein, and soft fibrin clots in the superior vena cava, the right atrium, the right ventricle and the pulmonary arteries. Thrombi were also found in the rami of the arteries in both lungs under the microscope. Leucocyte infiltration was recognized in the thrombus, but no organization. This finding shows that the thrombi were fresh. The remainder (150ml) of the drip solution was analyzed for thrombin activity and 8 to 16 units of thrombin activity per ml were demonstrated. From these results, the cause of death was considered pulmonary thrombosis due to intravenous administration of thrombin.

    DOI: 10.4044/joma1947.105.9-10_839

    CiNii Article

    CiNii Books

    researchmap

  • Unusual Mechanism of Ring Fracture of the Base of the Skull--A Case Report Reviewed

    MIYAISHI S, MORIYA F, YAMAMOTO Y, ISHIZU H

    Acta criminologiae et medicinae legalis Japonica   58 ( 6 )   p217 - 222   1992.12

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:日本犯罪学会  

    17歳女,交通事故により生じた頭蓋底輪状骨折の症例.本例では,頭部において体幹から上方向へ引き抜き動作により骨折が生じたと考えられた.本例の輪状骨折の原因は抜出の範ちゅうではあるが,頭部の横軸方向の回転は見られなかった

    CiNii Article

    CiNii Books

    researchmap

  • 特異なハンドル損傷を認めた1剖検例 Reviewed

    宮石 智, 守屋 文夫, 山本 雄二

    法医学の実際と研究   35   225 - 229   1992.12

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:法医学談話会  

    researchmap

  • 検案現場で使用可能な一酸化炭素ヘモグロビンの簡易半定量法 Reviewed

    宮石 智, 守屋 文夫, 山本 雄二

    法医学の実際と研究   35   141 - 145   1992.12

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:法医学談話会  

    死体検案中に実施可能な一酸化炭素ヘモグロビン(CO-Hb)の簡易半定量法を考案した.本法では微量拡散法の原理に基づき,密閉したバイアル内で血液から遊離させた一酸化炭素(CO)と,ろ紙に浸み込ませた塩化パラジウムの塩酸溶液を反応させる.その結果ろ紙上に金属パラジウム(Pd)が析出するので,その析出程度からCO-Hb濃度を判断する.本法によれば,CO-Hb濃度が20%以上の血液ではPdはろ紙全面に一様に析出するのに対し,10%の血液ではPdは斑状に析出する.COを吸引した死体の場合にはCO-Hb濃度は通常20%以上にはなるので,本法によりCO吸引の有無を検案中に知ることができ,死亡時の状況を把握したり死因を確定する上で役立つ

    researchmap

  • フグ中毒死の2剖検例 Reviewed

    宮石 智, 守屋 文夫, 山本 雄二

    法医学の実際と研究   35   177 - 182   1992.12

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:法医学談話会  

    researchmap

  • Presumption of a history of methamphetamine abuse by postmortem analyses of hair and nails: a case report. Reviewed

    F Moriya, S Miyaishi, H Ishizu

    Arukoru kenkyu to yakubutsu izon = Japanese journal of alcohol studies & drug dependence   27 ( 2 )   152 - 8   1992.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    By sectional analyses of hair, pubic hair, fingernail and toenail of the descendent who had been a habitual abuser of methamphetamine, the drug was detected in each specimen and the drug concentration in the section containing root was higher than that in the other sections. However, no methamphetamine was detected in the other body fluids and tissues. It was considered that the decedent had used methamphetamine for about 18 months and that the dosage of the drug used for the last 10 days or so was relatively large though he had discontinued injecting the drug for 3-5 days.

    PubMed

    researchmap

  • The use of mass fragmentography for the detection of tetrodotoxin in human body fluids. Reviewed

    F Moriya, S Miyaishi, Y Yamamoto, H Ishizu

    Nihon hoigaku zasshi = The Japanese journal of legal medicine   46 ( 2 )   117 - 20   1992.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The authors report on a convenient electron impact ionization-mass fragmentographic method for determining the amount of tetrodotoxin (TTX) present in body fluids. To explain this method briefly, the TTX present in body fluids was purified with activated charcoal and transformed into 2-amino-6-hydroxymethyl-8-quinazoline (C9-base) with alcoholic KOH (1%). Then the C9-base was injected into a gas chromatograph-mass spectrometer in the form of trimethylsilyl derivative. In this manner the minimum detectable amount of TTX in body fluids was found to be about 0.01 microgram/g. When this method was applied to sera, urine, and the stomach contents of 2 decedents suspected of puffer fish poisoning, 0.053 microgram/g of TTX was detected in stomach contents of one victim. This new analytic method is simpler and more sensitive than other assay methods and thus is considered useful in forensic work.

    PubMed

    researchmap

  • Polymerase chain reactionを用いた指紋の性別判定法 Reviewed

    山本 雄二, 守屋 文夫, 宮石 智

    医学のあゆみ   160 ( 7 )   481 - 482   1992.2

     More details

    Language:Japanese   Publisher:医歯薬出版(株)  

    researchmap

  • 著明な耐性形成が考えられたシンナー濫用者の1剖検例 Reviewed

    守屋 文夫, 石津 日出雄, 宮石 智

    法医学の実際と研究   34   151 - 154   1991.12

     More details

    Language:Japanese   Publisher:法医学談話会  

    木製バットで頭部を強打され,約49時間後に硬膜外血腫による脳圧迫により死亡したシンナー使用常習者の剖検例で,大脳,小脳,硬膜外血腫,左肺,右肺,大腿部筋肉,脂肪組織および右心血からそれぞれ16.5 μg/g, 6.22 μg/g, 3.08 μg/g, 0.39 μg/g, 0.59 μg/g, 1.06 μg/g, 26.6 μg/gおよび0.33 μg/gのトルエンが検出された.本屍の右心血中トルエン濃度を薬物速度論的に解析した結果,受傷時の血中トルエン濃度は31 μg/g以上であったと推定された.しかし,受傷するまでの行動から死者が重度のシンナー酩酊状態であったとは考えられず,シンナーに対する耐性が著明に形成されていた可能性も否定できない

    researchmap

  • Unusual productions of endogenous alcohol: report of two autopsy cases. Reviewed

    F Moriya, H Ishizu, S Miyaishi

    Arukoru kenkyu to yakubutsu izon = Japanese journal of alcohol studies & drug dependence   26 ( 5 )   373 - 9   1991.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We experienced two autopsy cases in which endogenous alcohol productions by saprogens had been unusual. Case 1 is a victim found in a mountain stream 3 to 4 weeks after his death. Since ethanol and n-propanol concentrations in the right intrathoracic bloody fluid, pericardial sac fluid, and blood in the right side of the heart were 0.41 mg/g and 0.052 mg/g, 0.42 mg/g and 0.032 mg/g, and 0.45 mg/g and 0.025 mg/g, respectively. The ethanol detected in those specimens appeared to have been produced postmortem. The femoral muscle and urine, however, contained very little n-propanol though the ethanol levels were 0.21 mg/g and 0.05 mg/g, respectively. Thus, we judged the victim might have died soon after drinking a little alcohol. Case 2 is a victim who was stabbed in the abdomen (liver) with a knife and died due to hemorrhagic shock after 26.5 hours in spite of a peritoneotomy. It was probable that metabolic activities of the liver had decreased significantly after getting a wound. Almost 1 mg/g of ethanol and little n-propanol were detected in the heart blood. In the intraabdominal bloody fluid, however, 2.45 mg/g of ethanol and 0.079 mg/g of n-propanol were detected. n-Propanol level in the bloody fluid is equal to that in severely decomposed body and indicates that a large amount of ethanol was endogenously produced. It may be considered that the unusual ethanol production was caused by the severe peritonitis after the operation.

    PubMed

    researchmap

  • An enzyme immunoassay for human myoglobin and its application to forensic medicine Reviewed

    S. Miyaishi

    Japanese Journal of Legal Medicine   45 ( 1 )   6 - 25   1991

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    We have devised an enzyme immunoassay for the determination of human myoglobin. This assay provides superb reproducibility and a recovery rate without the influence of any anti-coagulants and hemolysis. The mean blood myoglobin level in healthy humans, determined by this assay, was 35.5 ± 23.9 ng/ml and the urine myoglobin level was found to be distributed over a broad range under 373.5 ng/ml. In cases of trauma sustained by living bodies, the blood myoglobin level was found to increase in accordance to the severity of trauma. Further, the myoglobin level in the blood began its increase during the early stage of the injury, and greater increases were shown in the elderly. In autopsy specimens, the ratio of the cardiac blood myoglobin level to the cerebrospinal fluid myoglobin level had a negative correlation with the postmortem passage of time and, as in the case of living bodies, the myoglobin level in the blood was considered a useful indicator of the severity of damage. However, since myoglobin level was found to vary, depending on the sites from where the sample blood was taken, the evaluation of the data should be done carefully. Finally, the simultaneous determination of the creatine phosphokinase (CPK) activity in autopsied blood samples would be of help for estimating the pestmortem passage of time and the progress of putrefaction.

    Scopus

    PubMed

    researchmap

  • Blood myoglobin level after trauma and exercise

    MIYAISHI Satoru, KITAO Takashi, MORIYA Fumio, YAMAMOTO Yuji, ISHIZU Hideo, ISHII Hiroyuki

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   103 ( 9 )   1103 - 1107   1991

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:Okayama Medical Association  

    The blood myoglobin level was determined in 18 trauma cases of long bone fracture and the changes in the blood myoglobin level and activities of enzymes in the blood exuded from the muscle tissues after a slight exercise of a 2-km marathon of healthy persons were determined. The blood myoglobin level of the trauma cases increased while there were no changes in the blood myoglobin level and activities of the enzymes in the blood after a 2-km marathon. The blood myoglobin level may be of help to determine whether a man has been injured or not even if he had been exercising at the time of injury.

    DOI: 10.4044/joma1947.103.9-10_1103

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/1993053828

  • Sex identification of bloodstains in forensic sciences

    ISHIZU Hideo, YAMAMOTO Yuji, MORIYA Fumio, MIYAISHI Satoru, SEMBA Shozo

    Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   103 ( 3 )   145 - 153   1991

     More details

    Language:Japanese   Publisher:Okayama Medical Association  

    The progress of sex identification methods for forensic materials such as dried bloodstains and others in legal medicine is reviewed.<br>Dixson and Torr (1956) first described a scientific method to identify human sex from forensic materials by detection of X chromatin in female cell nuclei. In 1972, we introduced a method for detecting Y chromatin in the human interphase nuclei by fluorescent microscopy as a new tool for decisive male sex determination from forensic materials. This method was accepted in forensic practice as the only reliable means to identify sex. In 1983, we reported sexes were distinguishable from dried bloodstains by the ratio of sex hormones, testosterone and progesterone, determined by radioimmunoassay. Gene analytical methods to prove the human Y chromosome specific DNA sequences have been introduced in this field. We also examined the method of Witt and Erickson (1989) to detect Y and X chromosome specific sequences using the polymerase chain reaction (PCR), and we improved this technique to exhibit its ability to identify sex in forensic practice. Our improved PCR method is expected to have a broad applicability in forensic practice because of its simplicity, sensitivity and reliability.

    DOI: 10.4044/joma1947.103.3_145

    CiNii Article

    researchmap

  • 都市ガス中毒家族内発生の1例 Reviewed

    橋本 良明, 守屋 文夫, 宮石 智

    日本法医学雑誌   44 ( 5〜6 )   475 - 480   1990.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    台所のガステーブルからの都市ガスの漏れによる一酸化炭素中毒で,約4日後に発見された3名のうち1名は既に死亡していた

    researchmap

    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1990&ichushi_jid=J01194&link_issn=&doc_id=19870273010009&doc_link_id=40003040007&url=https%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F40003040007&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

  • Medico-legal aspects of traumatic injures-Rrport of 13 autopsy cases-

    102 ( 1 )   23 - 36   1990.2

     More details

    Language:Japanese  

    In this paper we present 13 autopsy cases on interest in medico-legal traumatology. The cases are as follows : 1)two cases of incomplete cardiac rupture dur to thoracic trauma, 2)rupture of herniated large intestine due to abdominal trauma, 3)large incised wounds on the side of the neck by a small kitchen knife, 4)a homicidal case with similar wounds that accompanied self-inflicted wounds, 5)a homicidal case accompanied by multiple stab wounds on the chest, 6)an accidental death skewered by a slender metallic stick, 7)special fatal shotgun injuries, 8)a homicidal case accompanid by various burns by an electric hair-dryer, iron and ciga-rettes, 9)two murder cases by a metallic baseball bat, 10)a suicidal case due to a fall from a high place, and 11)a severely disrupted victim of a road traffic accident.

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/1990165933

  • 水に関係ある死体とプランクトン検査の法医学的考察 Reviewed

    何川 涼, 宮石 智, 中井 三代子

    法医学の実際と研究   31   313 - 318   1988.12

     More details

    Language:Japanese   Publisher:法医学談話会  

    法医学の実務において水に関係した死体は多種多様であり,水中に限らず陸上や室内でもしばしば経験されるが,死因の決定に当たり剖検所見と共にプランクトン検査が参考にされる.このような死体を次のように分類することを提唱する.1)溺死,2)水中異常急死,3)水中での病死,4)水の気道内吸入による窒息死,5)末期呼吸期の水吸収,6)死体の水中放棄.1)〜5)は肺のプランクトンが陽性であってよい.しかし2)と5)ではプランクトンは少数で肺内の分布が不均一のことが多い.5)は先行病変を死因とすべきだと考える.4)を溺死と区別するのは,室内で水を気道に吸入するなど,溺死という語がなじまず,かつ死亡時の症状も異なるためである.6)は水の死後侵入がなければプランクトンは陰性である

    researchmap

▼display all

Books

  • 標準法医学 第8版

    宮石 智( Role: Contributor ,  窒息)

    2021.12 

     More details

  • 死体検案ハンドブック第4版

    宮石智( Role: Contributor ,  死体現象)

    金芳堂  2020.1 

     More details

    Responsible for pages:18-37   Language:Japanese

    researchmap

  • .

    ( Role: Joint editor ,  Editor)

    2019.9 

     More details

    Responsible for pages:145-149.   Language:English

    researchmap

  • Advances in Legal Medicine from a Japanese-German Perspective.

    Püschel K and Miyaishi S( Role: Contributor ,  10 x ISALM ? A Record of Japanese/German Mutual Understanding in Forensics.)

    Verlag Dr.Kova?  2019.9 

     More details

    Responsible for pages:145-149.   Language:English

    researchmap

  • ヘルスサービスリサーチ入門

    伊藤智子,田宮菜奈子,宮石智( Role: Contributor ,  関連領域との協働.4. 法医学.)

    東京大学出版会  2017.10 

     More details

    Language:Japanese

    researchmap

  • Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

    ( Role: Contributor)

    Reference Module in Chemistry, Molecular Sciences and Chemical Engineering.  2016 

     More details

    Language:English

    researchmap

  • Encyclopedia of Forensic and Legal Medicine、2nd ed.

    Miyaishi S, Moriya F( Role: Contributor ,  Immunoassays, Applications Forensic.)

    Elsecvier  2015 

     More details

    Language:English

    researchmap

  • 標準法医学 第7版

    宮石智( Role: Contributor ,  窒息)

    医学書院  2013  ( ISBN:9784260015929

     More details

    Responsible for pages:97-115   Language:Japanese

    researchmap

  • 根拠に基づく高齢者施設ケア

    ( Role: Contributor ,  異状死体への対処)

    金芳堂  2009.4 

     More details

    Responsible for pages:83-89   Language:Japanese

    researchmap

  • Encyclopedia of Forensic and Legal Medicine

    Miyaishi S and Moriya F( Role: Contributor ,  Immunoassays, overview of applications in forensic science.)

    Elsevier,Oxford  2005 

     More details

    Responsible for pages:pp73-79  

    researchmap

  • Encyclopedia of Analytical Science, 2nd Ed

    Miyaishi S and Moriya F( Role: Contributor ,  Immunoassays, forensic applications.)

    Elsevier,Oxford  2005 

     More details

    Responsible for pages:pp329-334  

    researchmap

  • Encyclopedia of Analytical Science

    ( Role: Contributor)

    Academic Press  1995.5 

     More details

    Responsible for pages:ReaD移行データ日付 : 1995  

    researchmap

▼display all

MISC

  • 胃内容中および血液中メタンフェタミン濃度の比較による覚せい剤摂取経路の推定

    吉留 敬, 守屋 文夫, 宮石 智

    日本法医学雑誌   73 ( 1 )   89 - 89   2019.5

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 平成30年7月豪雨災害における頭蓋3次元モデルを用いた頭蓋・顔写真スーパーインポーズ法による兄弟識別の1例

    土井 裕輔, 三浦 雅布, 谷口 香, 宮石 智

    日本法医学雑誌   73 ( 1 )   96 - 96   2019.5

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 吐物誤嚥により心臓血から致死濃度のアルコールが検出された1剖検例

    谷口 香, 竹居 セラ, 山崎 雪恵, 三浦 雅布, 守屋 文夫, 宮石 智

    日本法医学雑誌   73 ( 1 )   85 - 85   2019.5

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 初療時には診断をつけられなかった農薬中毒の2例

    有吉 雪乃, 内藤 宏道, 竹居 セラ, 森本 直樹, 三浦 雅布, 中尾 篤典, 宮石 智

    中毒研究   32 ( 1 )   100 - 100   2019.3

     More details

    Language:Japanese   Publisher:(株)へるす出版  

    researchmap

  • 相同モデル化理論を応用した口蓋形態による個人識別法

    岡田 俊輔, 柳 文修, 岡田 亜由美, 久富 美紀, 谷尻 豊寿, 飯田 征二, 宮石 智, 浅海 淳一

    Japanese Journal of Diagnostic Imaging   37 ( 1 )   89 - 89   2019.3

     More details

    Language:Japanese   Publisher:日本画像医学会  

    researchmap

  • 覚せい剤非経口摂取事例におけるメタンフェタミンの血中濃度と胃内容中濃度の関係について

    吉留 敬, 守屋 文夫, 三浦 雅布, 宮石 智

    中毒研究   32 ( 1 )   97 - 97   2019.3

     More details

    Language:Japanese   Publisher:(株)へるす出版  

    researchmap

  • 心臓血中アルコール濃度への誤嚥の影響 実際例からの考察

    竹居 セラ, 山崎 雪恵, 三浦 雅布, 有吉 雪乃, 守屋 文夫, 宮石 智

    中毒研究   32 ( 1 )   99 - 100   2019.3

     More details

    Language:Japanese   Publisher:(株)へるす出版  

    researchmap

  • 法医解剖における血液培養検査の意義 乳児剖検例について

    宮石 智, 三浦 雅布, 山崎 雪恵, 小林 智瑛, 池松 和哉

    日本法医学雑誌   72 ( 2 )   326 - 326   2018.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 統計からみた乳児突然死の死因解明状況に関する一考察

    山崎 雪恵, 谷口 香, 宮石 智

    法医病理   24 ( 2 )   114 - 115   2018.12

     More details

    Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 法医解剖における血液培養の有用性について

    小林 智瑛, 三浦 雅布, 山崎 雪恵, 宮石 智

    法医病理   24 ( 2 )   118 - 119   2018.12

     More details

    Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 岡山県における海上保安庁の死体取扱状況について

    三浦 雅布, 奥谷 麻実, 井上 惣太, 宮石 智

    法医病理   24 ( 2 )   119 - 120   2018.12

     More details

    Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 総肺静脈還流異常症による乳児突然死の2剖検例

    伊藤 啓, 三浦 雅布, 谷口 香, 山崎 雪恵, 宮石 智

    法医病理   24 ( 2 )   114 - 114   2018.12

     More details

    Language:Japanese   Publisher:日本法医病理学会  

    researchmap

  • 乳児剖検例における血液培養検査の結果に関する検討

    三浦 雅布, 山崎 雪恵, 小林 知瑛, 山本 雄二, 宮石 智

    日本法医学雑誌   72 ( 2 )   319 - 320   2018.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • ホルマリン固定中骨格筋からのミオグロビン漏出について

    小林 智瑛, 三浦 雅布, Thu Thu Htike, 山本 雄二, 宮石 智

    日本法医学雑誌   72 ( 2 )   320 - 320   2018.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 覚せい剤中毒死が疑われた肺脂肪塞栓症の一例

    吉留 敬, 三浦 雅布, 谷口 香, 守屋 文夫, 宮石 智

    日本法医学雑誌   72 ( 2 )   321 - 321   2018.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 横紋筋からのミオグロビンの死後漏出について ホルマリン固定中変化の検討

    宮石 智, 小林 智瑛, 三浦 雅布

    日本法医学雑誌   72 ( 2 )   325 - 326   2018.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 岡山県における5歳未満の不慮の事故死の特徴

    山崎 雪恵, 田宮 菜奈子, 山本 秀樹, 谷口 香, 三浦 雅布, 宮石 智

    日本法医学雑誌   72 ( 2 )   318 - 318   2018.12

     More details

    Language:Japanese   Publisher:(NPO)日本法医学会  

    researchmap

  • 乳幼児突然死症候群(SIDS)死亡率の年次変化と地域差に関する考察

    山崎 雪恵, 田宮 菜奈子, 山本 秀樹, 宮石 智

    日本公衆衛生学会総会抄録集   77回   319 - 319   2018.10

     More details

    Language:Japanese   Publisher:日本公衆衛生学会  

    researchmap

▼display all

Presentations

  • 腐敗した剖検組織試料のヘマトキシリン・エオジン染色像で観察される沈着物についての検討

    小林智瑛, 三浦雅布, 谷口香, 宮石智:

    第105次日本法医学会学術全国集会. 

     More details

    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ミオグロビンの死後血中移行メカニズム解明に関する予備的検討

    竹居セラ,小林智瑛,谷口香,三浦雅布,守屋文夫,宮石智.

    第105次日本法医学会学術全国集会. 

     More details

    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 認知症患者における外因死の実態把握への取り組み?法医剖検情報を用いて.

    三浦雅布, 谷口 香, 山?雪恵, 宮石 智.

    第105次日本法医学会学術全国集会. 

     More details

    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 嘔吐下痢症ののち急激な転帰をとった乳児突然死の一例

    谷口 香,竹居セラ,小林智瑛,山?雪恵,三浦雅布,宮石 智:

    第38回日本法医学会学術中四国地方集会 

     More details

    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 法医学から考古学への貢献 〜白骨鑑定を埋蔵文化財保護に結びつけた一例

    竹居セラ,小林智瑛,山?雪恵,谷口香,三浦雅布,宮石智.

    第38回日本法医学会学術中四国地方集会 

     More details

    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Beurteilung (einer t?dlichen?) Alkoholkonzentration in Obduktionsblutproben ? eine Kasuistik International conference

    Miyaishi S, Taniguchi K, Yamasaki Y, Miura M, Moriya F, P?schel K:

    100te Jahrestagung der Deutschen Geselschaft fuer Rechtsmedizin 

     More details

    Event date: 2021.9

    Language:German   Presentation type:Oral presentation (general)  

    Venue:Muenchen, Germany  

    researchmap

  • ミオグロビンの動脈への死後拡散に関する実験的検討

    竹居セラ,小林智瑛,谷口 香,山本雄二,宮石 智

    第37回日本法医学会学術中四国地方集会 

     More details

    Event date: 2020.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 血中覚せい剤の胃内移行機序解明に向けたラットを用いた実験系の構築

    吉留敬,守屋文夫,三浦雅布,宮石智

    第37回日本法医学会学術中四国地方集会 

     More details

    Event date: 2020.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ドイツハンブルグ大学法医学研究所におけるCOVID-19への対応から考える法医解剖の意義について

    三浦雅布,谷口 香,竹居セラ,宮石 智:

    第37回日本法医学会学術中四国地方集会 

     More details

    Event date: 2020.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 法医剖検腐敗組織試料を保存したホルマリンのpHについての検討

    小林智瑛, 三浦雅布, 山?雪恵,西本翔祐,宮石智:

    第37回日本法医学会学術中四国地方集会 

     More details

    Event date: 2020.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 化石珪藻と考えられるプランクトンが認められた溺死の1 例

    山本雄二,三浦雅布,小林智瑛,山崎雪恵,竹居セラ,谷口香,宮石智:

    第104次日本法医学会学術全国集会 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 法医解剖における気道培養と血液培養の有用性について

    小林智瑛,三浦雅布,山崎雪恵,宮石智

    第104次日本法医学会学術全国集会 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 法医剖検試料を用いた血液培養検査における,試料採取方法による検査結果の違いについて

    三浦雅布,小林智瑛,宮石 智

    第104次日本法医学会学術全国集会 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Myoglobinに関する法医学的研究

    宮石智

    第104次日本法医学会学術全国集会 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    researchmap

  • 乳児突然死剖検例におけるワクチン接種状況.

    増中彩香,山崎雪恵,宮石智,守屋文夫:

    第104次日本法医学会学術全国集会 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 血中覚せい剤の胃内移行機序解明に向けた動物実験系の構築

    吉留敬,守屋文夫,宮石智

    第104次日本法医学会学術全国集会 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 剖検によりG群溶連菌感染症が強く疑われた一例.

    谷口 香,三浦雅布,宮石智:

    第109回日本病理学会オンライン総会 

     More details

    Event date: 2020.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 血中メタンフェタミン濃度の死後変動について.

    吉留敬,守屋文夫,三浦雅布,宮石智:

    第31回日本中毒学会中国四国地方会 

     More details

    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Current practice of forensic medicine in Myanmar: characteristics and problems.

    Thu Thu Htike, Satoru Miyaishi:

    第2回日本法医病理学会学術全国集会 

     More details

    Event date: 2019.12

    Language:German   Presentation type:Oral presentation (general)  

    researchmap

  • 指定外容器に保存した尿を用いたミオグロビン濃度定量の意義

    竹居セラ,小林智瑛,三浦雅布,山?雪恵,宮石智:

    第2回日本法医病理学会学術全国集会 

     More details

    Event date: 2019.12

    Language:German   Presentation type:Oral presentation (general)  

    researchmap

  • An autopsy case of cyanide poisoning.

    Thu Thu Htike, Fumio Moriya, Satoru Miyaishi:

    第31回日本中毒学会中国四国地方会 

     More details

    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 簡易薬物スクリーニングキットIVeX-screen M-1の法医解剖検査における有用性について

    竹居セラ,,山?雪恵,守屋文夫,宮石智:

    第31回日本中毒学会中国四国地方会 

     More details

    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 頸動脈洞反射による急性心停止と考えられた頸部圧迫の一剖検例.

    宮石智,竹居セラ,Thu Thu Htike,小林智瑛,植木安菜,山本雄二,谷口 香:

    第36回日本法医学会学術中四国地方集会. 

     More details

    Event date: 2019.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Cyanide concentrations in blood and tissues of fire victims International conference

    Thu Thu Htike, Moriya F, Kobayashi C, Miura M, Miyaishi S:

    98te Jahrestagung der Deutschen Geselschaft fuer Rechtsmedizin 

     More details

    Event date: 2019.9

    Language:German   Presentation type:Oral presentation (general)  

    Venue:Hamburgn, Germany  

    researchmap

  • A study on usefulness of lower respiratory tract culture and blood culture in forensic autops International conference

    Kobayashi C, Miura M, Yamasaki Y, Thu Thu Htike, Miyaishi S. y

    98te Jahrestagung der Deutschen Geselschaft fuer Rechtsmedizin 

     More details

    Event date: 2019.9

    Language:German   Presentation type:Oral presentation (general)  

    Venue:Hamburgn, Germany  

    researchmap

  • The report for a large number of postmortem examinations caused by The Heavy Rain Event of July 2018 in Mabi-cho Kurashiki-city (Japan) International conference

    Miura M, Yamasaki Y, Kobayashi C, Thu Thu Htike, Taniguchi K, Yamamoto Y, Miyaishi S: .

    98te Jahrestagung der Deutschen Geselschaft fuer Rechtsmedizin 

     More details

    Event date: 2019.9

    Language:German   Presentation type:Oral presentation (general)  

    Venue:Hamburgn, Germany  

    researchmap

  • 経口・非経口投与されたメタンフェタミンの血中および胃内動態:ラットを用いた検討

    吉留敬,守屋文夫,宮石智: .

    日本法中毒学会第38年会 

     More details

    Event date: 2019.7

    Language:German   Presentation type:Oral presentation (general)  

    Venue:Hamburgn, Germany  

    researchmap

  • 動物実験モデルを用いた血中覚せい剤の胃内移行動態に関する予備的研究

    吉留敬,守屋文夫,宮石智:

    日本法中毒学会第39年会 

     More details

    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 尿中ミオグロビン測定値に対する保存容器の影響について

    小林智瑛, 三浦雅布, 山?雪恵, 宮石智:

    第103次日本法医学会学術全国集会. 

     More details

    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Determination of cyanide concentration in autopsy materials

    Thu Thu Htike,守屋文夫,宮石 智:

    第103次日本法医学会学術全国集会. 

     More details

    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 吐物誤嚥により心臓血から致死濃度のアルコールが検出された 1 剖検例

    谷口 香,竹居セラ,山崎雪恵,三浦雅布,守屋文夫,宮石智:

    第103次日本法医学会学術全国集会. 

     More details

    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 平成 30 年 7 月豪雨災害における頭蓋 3 次元モデルを用いた頭蓋・顔写真スーパーインポーズ法による兄弟識別の 1 例

    土井裕輔,三浦雅布,谷口香,宮石 智:

    第103次日本法医学会学術全国集会. 

     More details

    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 胃内容中および血液中メタンフェタミン濃度の比較による覚せい剤摂取経路の推定

    吉留敬,守屋文夫,宮石智:.

    第103次日本法医学会学術全国集会. 

     More details

    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 相同モデル化理論を応用した口蓋形態による個人識別

    岡田俊輔,柳文修,岡田亜由美,久富美紀,谷尻豊寿,飯田征二,宮石智,浅海淳一

    第38回日本画像医学会学術集会 

     More details

    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Cyanide concentration in autopsy materials International conference

    Thu Thu Htike, Fumio Moriya, Satoru Miyaishi

    47th Myanmar Health Research Congress 

     More details

    Event date: 2019.1

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • Medico-legal management of mass disaster: An experience in Okayama, Japan, 2018 International conference

    Thu Thu Htike, Miura M, Taniguchi K, Yamasaki Y, Kobayashi C, Yamamoto Y, Miyaishi S:

    47th Myanmar Health Research Congress 

     More details

    Event date: 2019.1

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 初療時には診断をつけられなかった農薬中毒の2例

    有吉雪乃,内藤宏道,竹居セラ,森本直樹,三浦雅布,中尾篤典,宮石智

    第30回日本中毒学会中国四国地方会 

     More details

    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 心臓血中アルコール濃度への誤嚥の影響 ?実際例からの考察.

    竹居セラ,山?雪恵,三浦雅布,有吉雪乃,守屋文夫,宮石智

    第30回日本中毒学会中国四国地方会 

     More details

    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 覚せい剤非経口摂取事例におけるメタンフェタミンの血中濃度と胃内容中濃度の関係について

    吉留 敬,守屋文夫,三浦雅布,宮石 智:

    第30回日本中毒学会中国四国地方会 

     More details

    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 乳幼児突然死症候群 (SIDS) 死亡率の年次変化と地域差に関する考察.

    山崎雪恵, 田宮菜奈子, 山本秀樹, 宮石智:

    第77回日本公衆衛生学会総会 

     More details

    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 平成30年7月豪雨における倉敷市真備町の多数死体検案についての報告.

    三浦雅布,山?雪恵,Thu Thu Htike,小林智瑛,山本雄二,谷口 香,宮石 智:

    第35回日本法医学会学術中四国地方集会, 

     More details

    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 胃内容と血液のメタンフェタミン濃度の関係に及ぼすメタンフェタミン投与経路の影響.

    吉留敬,守屋文夫,宮石智

    第35回日本法医学会学術中四国地方集会, 

     More details

    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 相同モデル化理論を応用した個人識別法?口蓋形態を利用して.

    岡田俊輔,宮石智,柳文彦:

    第35回日本法医学会学術中四国地方集会, 

     More details

    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 乳児急死剖検例の助産学的検討:妊産婦に対する保健指導への還元を目指して(第2報)

    松永梨花,宮石智,守屋文夫:

    第35回日本法医学会学術中四国地方集会, 

     More details

    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 化石珪藻と考えられるプランクトンが認められた溺死事例.

    山本雄二,難波令匡,井澗美希,吉留 敬,三浦雅布,宮石智:

    第35回日本法医学会学術中四国地方集会, 

     More details

    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • An autopsy case of sudden death of Cornelia de Lange syndrome.

    Thu Thu Htike,Taniguchi K,Miura M, Miyaishi S:

    第1回日本法医病理学会学術全国集会 

     More details

    Event date: 2018.9

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • A method for personal identification by palate morphology using homologous modeling. International conference

    Okada S, Okada A, Hisatomi M, Takeshita Y, Matsubara R, Tanijiri T, Iida S, Miyaishi M, Asasumi J,Yanagi Y

    12th Asian Congress of Oral & Maxillofacial Radiology 

     More details

    Event date: 2018.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Mumbei, India  

    researchmap

  • Stabilit?t des Zyanides in postmortalem Blut International conference

    Miyaishi S, Thu Thu Htike, Yamasaki Y, Kobayashi C, Miura M, Moriya F

    97te Jahrestagung der Deutschen Geselschaft fuer Rechtsmedizin 

     More details

    Event date: 2018.9

    Language:German   Presentation type:Oral presentation (general)  

    Venue:Halle, Germany  

    researchmap

  • 岡山県における海上保安庁の死体取扱状況について.

    奥谷麻実,三浦雅布,井上惣太,宮石 智

    第1回日本法医病理学会学術全国集会 

     More details

    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 総肺静脈還流異常症による乳児突然死の2剖検例.

    伊藤啓,三浦雅布,谷口香,山?雪恵,宮石智

    第1回日本法医病理学会学術全国集会 

     More details

    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 統計からみた乳児突然死の死因解明状況に関する一考察

    山崎雪恵, 谷口香, 宮石智

    第1回日本法医病理学会学術全国集会 

     More details

    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 法医解剖における血液培養の有用性について.

    小林智瑛,三浦雅布,山?雪恵,宮石智:

    第1回日本法医病理学会学術全国集会 

     More details

    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 死体血中覚せい剤濃度の部位依存性に関する研究.

    吉留敬,守屋文夫,三浦雅布,宮石智:

    日本法中毒学会第37年会 

     More details

    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Descriptive epidemiology of fatal single-bicycle accidents in Okayama prefecture, Japan International conference

    Yamasaki Y, Miura M, Miyaishi S

    24th Congress of the International Academy of Legal Medicine (IALM). 

     More details

    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • Comparative studies on the concentrations of oral and intravenous methamphetamine in stomach contents and blood International conference

    Yoshitome K, Moriya F, Miyaishi S

    24th Congress of the International Academy of Legal Medicine (IALM). 

     More details

    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • Medicosocial Aspects of Methamphetamine-Related Deaths. International conference

    Moriya F, Yoshitome K, Miyaishi S

    24th Congress of the International Academy of Legal Medicine (IALM). 

     More details

    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • Stability of cyanide in postmortem blood International conference

    Thu Thu Htike, Miyaishi S, Yamasaki Y, Kobayashi C, Miura M, Moriya F

    24th Congress of the International Academy of Legal Medicine (IALM). 

     More details

    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • Exudation of myoglobin from striated muscle during formalin fixation. International conference

    Kobayashi C, Yamasaki Y, Miura M, Miyaishi S

    24th Congress of the International Academy of Legal Medicine (IALM). 

     More details

    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • A study on practical usefulness of blood culture in forensic autopsy International conference

    Miura M, Yamasaki Y, Kobayashi C, Miyaishi S

    24th Congress of the International Academy of Legal Medicine (IALM). 

     More details

    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

▼display all

Awards

  • 欧文誌(小児医学研究振興財団アワード)

    2017  

     More details

    Country:Japan

    researchmap

  • ポスターアワード(法医病理学部門)

    2008  

     More details

    Country:Japan

    researchmap

  • ポスターアワード(法医病理学部門)第1位

    2002  

     More details

    Country:Japan

    researchmap

  • ポスターアワード(法医中毒学・法医生物学部門)第1位

    2002  

     More details

    Country:Japan

    researchmap

Research Projects

  • Immunoassay in Forensic Medicine -its Revival and Succession

    Grant number:21H03213  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    宮石 智, 谷口 香, 三浦 雅布, 高田 智世

      More details

    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

    researchmap

  • Forensic Toxicological Study on Excretion of Intravenously Administered Drugs into the Stomach

    Grant number:26460893  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MORIYA Fumio

      More details

    Grant amount:\1690000 ( Direct expense: \1300000 、 Indirect expense:\390000 )

    Our analytical findings on drugs in forensic autopsy cases demonstrated that: (1) basic drugs such as methamphetamine and ephedrine that are excreted into urine as unmetabolized form into urine at a large proportion appear at a higher concentration in urine than in the stomach when administered intravenously, and basic drugs that are mostly excreted into urine as metabolized form appear at a higher concentration in the stomach than in urine after intravenous administration; and (2) metabolites of a basic drug which increase gradually in blood are rapidly excreted into the stomach if they preserve basic character.
    An animal experiment using rabbits demonstrated that flecainide, a basic drug, is rapidly excreted into the stomach when it is intravenously administered. We established an animal model using rats for investigating excretion patterns of methamphetamine into the stomach.

    researchmap

  • A study on identifying unknown cause/factor of sudden infant death

    Grant number:25670343  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    MIYAISHI Satoru, KITAO TAKASHI, TAKATA TOMOYO, MIURA MASANOBU, TANIGUCHI KAORI, YAMASAKI YUKIE

      More details

    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    Unknown factors or diseases that were not recognized to cause sudden infant death were investigated. It was thought that infection of Cronobacter sakazaki as cause of sudden death could be simply diagnosed only by the growth of that pathogen in the culture. Fulminant type Ⅰ diabetes could also be diagnosed by the extreme low level of blood 1,5-AG concentration, whereas evaluation of stress as the factor of sudden death should be examined further using saliva chromogranin A concentration.
    According to the investigation of the ratios of area of the left ventricle to those of the right ventricle and the total left heart in the heart cross-section, it was suggested that the the left heart dysfunction could be another possible unknown factor/cause of infantile sudden death.

    researchmap

  • The Implementation of Regional Comprehensive Care through Field-based Health Services Research: The integration of Welfare, Medical and long-term Care

    Grant number:24249031  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    TAMIYA Nanako, NOGUCHI Haruko, MATSUI Kunihiko, MIYAISHI Satoru, YAMAZAKI Kentaro, YAMAMOTO Hideki, SAITO Tamaki, ABE Toshikazu, TAKEDA Fumi, TAKAHASHI Hideto, KASHIWAGI Masayo, IZUMIDA Nobuyuki, MATSUMOTO Yoshio, SHIBAYAMA Taiga, SANKAI Tomoko, ABE Yoshiki, ASO Hideki, KIKUCHI Jun, MORIYAMA Yoko, YAMAOKA Yui, ITO Tomoko, KOBAYASHI Yoko

      More details

    Grant amount:\45630000 ( Direct expense: \35100000 、 Indirect expense:\10530000 )

    This study is aimed at implementing the PDCA cycle (process improvement technique consisting of 4 steps:Plan,Do,Check, and Act) which has two main components, medical, long term care, and welfare care fieldsites and the university. We have planned to cooperate with the medical, long term care, welfare fieldsites by examining together the Tsukuba-city medical welfare case examination meetings, and providing feedback to Tsukuba City by analyzing their needs survey. We performed a scientific study using several types of data analysis, including big data from national long-term care benefit expenditures data and from the Comprehensive Survey of Living Conditions. We examined the regional comprehensive care promotion policy , including these findings, to clarify how to advance the cooperation between medical and long-term care, what kind of support require the persons in need of care, children with disabilities and the use situation of nursing care insurance services.

    researchmap

  • Proposal of suicide prevention strategy and implementation of an epidemiological study based on case reports of all suicides in Okayama prefecture

    Grant number:21590695  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    HAMADA Jun, TSUDA Toshihide, YORIFUJI Takashi, MIYAISHI Satoru

      More details

    Grant amount:\3380000 ( Direct expense: \2600000 、 Indirect expense:\780000 )

    By analyzing all the suicide cases in Okayama prefecture, we showed that suffocation suicides(rentan jisatsu) tend to occur during a certain season among men in their 30s to 40s. By creating a data set from the Vital Statistics, which has been compiled by the Ministry of Health, Labour and Welfare, we sought to identify vulnerable population to economic contraction in order to implement preventive interventions among them to reduce suicide. In addition, by analyzing suicide prevention strategies in advanced regions(e. g., Akita prefecture, Iwate prefecture, and Adachi-ku), we also demonstrated a significance of implementing their health promotion approaches in urban areas.

    researchmap

  • For a peaceful epilogue of life : An integrated outcome research center based on public health, forensic medicine and law

    Grant number:21390202  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    TAMIYA Nanako, MOTOZAWA Miyoko, NAKATANI Yoji, HONDA Katsuya, TAKAHASHI Hideto, MIYAISI Satoru, YAMAZAKI Kentaro, YAMAMOTO Hideki, KASHIWAGI Masayo, MATSUZAWA Akemi, MIYATA Sumiko, ITOU Tomoko, TANIGUCHI Kiyo, KANO Tomoko, MORITA Nobuaki, WAKINO Kotaro

      More details

    Grant amount:\18070000 ( Direct expense: \13900000 、 Indirect expense:\4170000 )

    A peaceful epilogue to our lives is what every human-being desire.
    However, in our rapid-aging country, there are many cases in which it cannot beaccomplished, such as in the case of a solitary death, by being abused, anaccident, or difficulty in dying at home. In this research, the actual conditions of these avoidable deaths in our country were explored by means of an interdisciplinary research with a health service research, and forensic medicine and a legal viewpoint.
    As a result, the community mutually supports itself based on an understanding of the dementia or psychiatric disorders, and a safe environmental management (the safety of farm machines etc) of the area. It was suggested that the medical welfare network in the community, such as the improvement of the role of the home support clinics or the end-of life care in the facilities, was very important.

    researchmap

  • Establishment of molecular forensic diagnosis related with cytokines and chemokines

    Grant number:20249040  2008 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    KONDO Toshikazu, KIMURA Akihiko, ISHIDA Yuko, MIYAISHI Satoru, IKEMATSU Kazuya

      More details

    Grant amount:\50700000 ( Direct expense: \39000000 、 Indirect expense:\11700000 )

    In order to establish novel molecular forensic diagnosis, we focus on cytokines/chemokines as the marker of systemic vital reactions, and examined the systemic dynamics of cytokines/chemokines in the various models of diseases, drug intoxication, and injuries, which are often encountered in forensic practices. We could find the several candidates cytokines/chemokines as key players in the models of sepsis, drowning, drug intoxication (acetaminophen, arsenite, cisplatin, chlorpromazine), deep vein thrombosis, acute pancreatitis, traumatic shock, and ischemia-reperfusion skeletal muscle injury, and identify specifics markers for molecular forensic diagnosis.

    researchmap

  • 法医公衆衛生学の構築-根拠に基づく医療福祉政策立案の新しい方法論

    Grant number:18659180  2006 - 2008

    日本学術振興会  科学研究費助成事業 萌芽研究  萌芽研究

    田宮 菜奈子, 宮石 智, 山本 秀樹, 松澤 明美, 本澤 巳代子

      More details

    Grant amount:\3300000 ( Direct expense: \3300000 )

    本研究では、法医学関連の情報を公衆衛生学的に分析し、実態に即した解決策を検討する新学問領域「法医公衆衛生学」の構築を試みた。これまでの3年間の経過を通じ、法医学関連情報として「法医剖検事例」の分析および「死体検案例」の分析双方を有機的に結びつけ分析することで、jこれまで実態が不明であった「虐待」「孤独死」「事故」「自殺」などの実態および関連要因を分析できることが明らかになった。「法医剖検事例」においては、事情聴取があるため、社会的背景など、フォームをあらかじめ作成し聴取する依頼をすることにより詳細な背景データが入手できた。しかし、火災は剖検率が高いが、病死は少ないなど、サンプリングバイアスがあり、剖検例の発生割合をそのまま公衆衛生学的な事例の発生割合と解釈することはできない。一方、「死体検案例」は、状況が不明な死のかなりの部分をカバーしていると考えられ、情報には限界があるが、全体の数および内訳分布などを把握するには適していると考えられた。
    具体的分析成果としては、まず、「剖検例からみた高齢者死亡の実態と背景要因」として、高齢者の剖検事例を対象として、家族形態によって状況が異なり、家族がいても事故などの異状死体が多いこと、高齢者の火災死亡には、コンロや灯明など特有の要因があり環境整備で予防しうるものがあることなどを、原著論文として発表した(厚生の指標56,1-7,2009)。2年目から開始したフォームによる剖検事例の背景情報の把握も蓄積され、最終年度に高齢者の死亡を分析し学会発表(日本公衆衛生学会2008)を、また、小児虐待の実態把握も行った。
    「死体検案例」については、まずは前述の死因別の剖検率を算出し、とくに火災では高いなど死因により異なることを、また死亡から発見までの期間による分類では、死因、発見場所、家族状況によって異なること、一方、自殺は家族同居者に多いことなどが明らかになった。
    また本研究班会議を開き、これらの分析結果を地方行政関係者と共有し、このように各種事例の属性別のパターンを明らかにすることで、「発生予防」および「早期発見」に分けた具体的政策も立案しやすいことが明らかになった。

    researchmap

  • Study on the practical usefulness of myoglobin from the aspects of forensic diagnosis

    Grant number:15390214  2003 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    MIYAISHI Satoru, ISHIZU Hideo, YAMAMOTO Yuji, YOSHITOME Kei, KITAO Takashi, TAKATA Tomoyo

      More details

    Grant amount:\10700000 ( Direct expense: \10700000 )

    Postmortem change of blood myoglobin concentration was experimentally investigated using rabbits. We encountered peculiar specimens two times during the term of the project, which we could not determined the assayed value. Although we could neither find the reason nor establish the concrete criterion for the estimation of time since death from the myoglobin level in postmortem blood, it was concluded that this method could be useful for the determination the time within about 12 hours postmortem. In the experiment of determining postmortem interval by the quantitative analysis of DNA degradation in myoglobin gene using mouse, it became clear that the amount of amplified products had depended on the postmortem interval, the length of the fragment amplified and the temperature in which the dead body had been left. This analysis was thought to be useful for estimating the postmortem interval of some weeks after death. Such principle like this seemed to be applicable to forensic autopsy cases. Postmortem exudation of myoglobin from the striated muscle and its diffusion were also experimentally investigated using mouse. Deletion of myoglobin was observed in the striated muscle by immunostaining as very early stage of postmortem change and this substance became visible again somewhat later. Such exudation of myoglobin could occur during the routine formalin-fixation and it was suggested that the same postmortem change could be observed about some other proteins in striated muscle. This means that the criteria of microscopic diagnosis of some causes of death using formalin-fixed myocardium/skeletal muscle must be revised.

    researchmap

  • Development of a high throughput SNP typing method using capillary electrophoresis

    Grant number:13470102  2001 - 2003

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ISHIZU Hideo, YOSHITOME Kei, MIYAISHI Satoru, YAMAMOTO Yuji, INAGAKI Sachiyo

      More details

    Grant amount:\13200000 ( Direct expense: \13200000 )

    Single nucleotide polymorphisms (SNP), the most numerous DNA markers in the human genome, have attracted the attention in many research fields.
    In this research project, we have attempted to construct the method for SNP typing by a multiplexed single nucleotide primer extension reaction and capillary electrophoresys. Using this method, we could establish a method for genotyping of ABO blood groups, a method for genotyping of HLA-DRB1, a method for multiplex SNPs typing of Y chromosome, and an efficient method which realizes 37-plex typing of SNPs on each chromosome. The results obtained were as follows.
    1. ABO genotypes of 103 Japanese could be determined by this method, and the estimated allele frequencies were agreed with the previous reports. We applied this method to casework samples, and successfully determined ABO genotypes of bones, teeth, muscles, organs, nails, and semen-contaminated vaginal contents.
    2. We examined 15 different SNP loci on Y chromosome in 159 Japanese males, and detected 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be applied for personal identification and patenity testing.
    3. We could establish a method for genotyping of HLA-DRB1 by the present method, and the method was useful particularly in the analysis of mixed specimens.
    4. We have establish an efficient method which realizes 37-plex typing of SNPs on each chromosome. This method holds promise as an effective means for analyzing forensic specimens and provides us with high discrimination power.

    researchmap

  • 母体末梢血からの胎児DNA多型分析法の開発

    Grant number:12877076  2000 - 2001

    日本学術振興会  科学研究費助成事業 萌芽的研究  萌芽的研究

    石津 日出雄, 吉留 敬, 宮石 智, 山本 雄二

      More details

    Grant amount:\2000000 ( Direct expense: \2000000 )

    本年度の研究経過および成績は以下に示すとおりである。
    1.妊娠後期の妊婦母体末梢血を5ml採取した。尚、血液採取に当たっては問診により過去の妊娠歴、流産歴、輸血歴等を明らかにしておくと共に、本研究は実験的段階であること及び病的遺伝子の診断ではないことを十分に説明し、採取に対するインフォームドコンセントおよびプライバシーの厳守など倫理面での配慮を欠かないようにした。
    2.まず妊婦末梢血を滅菌生理食塩水で2倍に希釈し、これを比重を1.075および1.085に調製した比重遠心分離液Percollに重層して1600回転で30分間遠心した後、中間層の有核赤血球を多く含有する分画を回収した。
    3.次に回収した有核赤血球を滅菌生理食塩水で洗浄後、DAB染色を施し、洗浄後さらに攪拌しながらエタノールを滴下して固定した。
    4.エタノール固定した有核赤血球の生理食塩水浮遊液にヘマトキシリンを添加して染色を施した後、その一部をスライドグラス上に載せ、マイクロマニピュレータをセットした顕微鏡で観察して有核赤血球を回収した。
    5.マイクロマニピュレーション法により回収した有核赤血球を1個ずつ個別のチューブに取り、Proteinase Kを含有するPCR緩衝液を添加して細胞溶解後、加熱によりProteinase Kを失活させ、チューブに新たにPEP(Primer Extension Preamplification)用プライマーまたはDual-PCR用プライマーを含むPCR反応混液を加えて反応させ、この反応産物の一部を鋳型としてPCR法によりSTR多型のタイピングを行った結果、一部のチューブにおいて、STR多型のタイピングが可能であった。また、母親のDNA型をリンパ球抽出DNAにより同時にタイピングして、有核赤血球から検出されたDNA型が母親のものとは異なることを確認した。
    6.また、胎児有核細胞からのタイピング結果を確認するために、出生後の新生児から少量の血液を採取し、DNA型をリンパ球抽出DNAによりタイピングして、有核赤血球から検出されたDNA型が出生後の新生児のDNA型と一致することを確認した。

    researchmap

  • STUDIES ON MUSCLE-ASSOCIATED SUBSTANCES FOR THE MEDICO-LEGAL DIAGNOSIS

    Grant number:10470119  1998 - 2000

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).  Grant-in-Aid for Scientific Research (B).

    ISHIZU Hideo, KITAO Takashi, YAMAMOTO Yuji, MIYAISHI Satoru

      More details

    Grant amount:\11600000 ( Direct expense: \11600000 )

    The contents of muscle-associated substances (MAS) in the forensic specimens were investigated. The content of cardiac troponin T (TnT) in myocardium was different among the investigated cases and had no correlation with the concentration of this protein in cardiac blood, as well as that of myoglobin (Mb). The contents of MAS of skeletal muscle were also different among cases and 19 sites investigated. Therefore the usefullness of the contents of MAS in muscle for medico-legal diagnosis in forensic autopsy could not be proved. Concentration of TnT and cardiac troponin I was higher in central/arterial blood than in peripheral/venous blood. CPK and creatine, that are more contained in skeletal muscle than in myocardium, were determined more in peripheral/venous blood than in central/arterial blood. Activities of GOT and GPT in the blood of vena cava inferior was higher than in blood obtained form any other site. These results may elucidate the state of postmortem circulation and diffusion of MAS or drugs in case of intoxication. When the MTAS concentration in blood or other body fluids was investigated from medico-legal aspects, the TnT and TnI concentrations in right and left heart blood were useful as a supplemental parameters to diagnose drowning. The creatinine concentration in pricardial fluid and the creatine concentrations in cerebrospinal fluid and vitreous humor were also useful for estimating postmortem interval. By detection of the human-specific sequence in the 3rd exon of Mb gene it was possibe to identify the human origin from forensic biological materials. The analysis of SNPs in the 2nd exon of Mb gene were useful for forensic personal identification and paternity test.

    researchmap

  • 挫減症候群におけるミオグロビンの毒性に関する実験的研究

    Grant number:09770300  1997 - 1998

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    宮石 智

      More details

    Grant amount:\2000000 ( Direct expense: \2000000 )

    Bywatersによって50年以上も前(1941年)に報告されていた挫滅症候群は、阪神淡路大震災を契機として、その重要性が再び認識されている。しかし、Bywatersが提唱した挫滅症候群の病態へのミオグロビンの関与については、今日なお解明が進んでいない。この点に着目した私の研究は、本研究補助金を得て、昨年度には、高純度ウサギミオグロビンの抽出・精製方法の確立とその大量抽出、これを抗原とした抗ウサギミオグロビンポリクローナル抗体(ヤギ)の作製、ウサギミオグロビンと交差反応性のある市販抗ヒトミオグロビンモノクローナル抗体の特定と、大きく前進した。本年度は、これらの成果に立脚して、先ずウサギミオグロビンのエンザイムイムノアッセイを確立した。すなわち、市販抗ヒトミオグロビンモノクローナル抗体を固相抗体、抗ウサギミオグロビンポリクローナル抗体(ヤギ)を二次抗体、市販のベルオキシダーゼ標識抗ヤギIgG抗体を検出抗体とするダブルサンドイッチELISAを完成した。次いで、挫滅症候群におけるミオグロビンの毒性を実験的に調べるための基礎的検討として、健康なウサギについて血中ミオグロビン濃度を調べたところ、10〜60ng/ml程度であった。更に、昨年度抽出したウサギミオグロビンを1mg/mlの溶液に調整し、これをlmg/kgの割合でウサギに経静脈投与した後、ミオグロビン被投与ウサギの血中ミオグロビン濃度を経時的に追跡した。その結果、投与直後の血中ミオグロビン濃度は約40μg/ml(正常値の1000倍程度)に上昇していたが、その後速やか低下し、30分後には約1μg/ml、.1時間後で約500ng/mlとなり、12時間後にはほぼ正常値に復した。このことから、挫滅症候群でミオグロビンが毒性を発現するのは、更に多量のミオグロビンが血中に放出される場合か、或いはミオグロビン以外の筋肉内成分との共存下の場合であることが示唆された。

    researchmap

  • Forensic molecular biological studies of sex chromosomal DNA for the application to criminal and civil identification

    Grant number:09557039  1997 - 1998

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    ISHIZU Hideo, NAKAMURA Chiemi, MIYAISHI Satoru, YAMAMOTO Yuji

      More details

    Grant amount:\11500000 ( Direct expense: \11500000 )

    1. Sex determination by detection of Y chromosome-specific aiphoid repeat sequence
    We have established the conditions for sensitive amplification of the Y chromosome-specific aiphoid repeat sequence by PCR, and succeeded sex determination from a hair shaft of 2 cm in length.
    2. Detection of Y chromosomal microsatellite polymorphisms
    The allele frequency of a microsatellite DYS 19 locus was estimated to be A=0.064, B=0.068, C=0.423, D=0.226, E=0.211, F=0.004 and C-2=0.004 (N=263) in Japanese living in Okayama and Hiroshima Prefectures. In these alleles, a C-2 allele, migrated between B and C alleles on electrophoretic gels, have not yet been reported in all the races. By the examination of family samples, the usefulness of this STR system in paternity tests was confirmed. Moreover, this STR system was useful for the identification of an assailant/assailants of a sexual assault. The other STR loci on the Y chromosome, DYS 390, DYS391 and DYS395 were also suggested to be useful in paternity tests and in sexual assault cases.
    3. Detection of an X chromosome microsatellite HPRT polymorphism
    The allele frequency of a microsatellite at HPRT locus on the X chromosome was estimated to be T1=0.105, T24=0.243, T3=0.396, T4=0.200 and T5=0.057 (male=84, female=63) in Japanese living in Okayama Prefecture. This STR locus was suggested to be useful in paternity tests.
    4. Detection of the amelogenin gene
    We have examined PCR products from various animals including human male and female amplified using primers for a human amelogenin sequence by restriction enzyme analysis and sequencing, and it was suggested that discrimination between those animals could be possible except that the animals were close phylogenetically. Moreover, using fluorescent dye-labeled primers, human male and female could be determined with high sensitivity by PCR.

    researchmap

  • 外傷死体における尿中ミオグロビン濃度測定の法医診断的意義

    Grant number:08770311  1996

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    宮石 智

      More details

    Grant amount:\900000 ( Direct expense: \900000 )

    殺人事件や交通轢逃げ事件の全貌を解明するには、犯行や事故発生の時刻を明らかにすることが極めて重要である。しかし、このような時刻の特定は、被疑者の自白や目撃証言という人間の記憶に依存しているのが実状である。従って、外傷死体の受傷後の生存時間を死体から推定し、延いては受傷時刻を特定することができれば法医学的に意義深い。ところで、筋肉内蛋白のミオグロビン(Mb)は、筋肉が損傷を受けると血中へ洩出し、更に尿中へ排泄される。そこで私は、外傷死体の尿中Mb濃度を独自に開発した酵素免疫測定法によって定量し、その値から当該死体の受傷後の生存時間を推定し得るか否かに付いて検討を加えた。まず対照用として収集した生体随時尿531例の尿中Mb濃度は大部分が20ng/ml以下であったが、100ng/ml以上の例も散見された。一方損傷による死亡例については、剖検ないし検案死体から54例の尿中Mb濃度を測定したところ、同濃度が500ng/ml以上のもの11例、1000ng/ml以上のもの5例が見いだされた。尿中Mb濃度と受傷から死亡までの経過時間との関係をみると、一般に強い損傷を受けたのち一定時間経過して死亡したものでは尿中Mb濃度が高い(例:16歳男性。轢き逃げ事故。第1車に跳ねられたうえ第2車に轢過され、約8時間後に死亡。尿中Mb濃度4610ng/ml)傾向にあった。しかしながら、受傷後の一定時間生存していても頭部外傷による死亡の場合(例:74歳男性。頭部だけをステッキで滅多打ちにされ、脳挫傷で約12時間後に死亡。尿中Mb濃度6ng/ml)や、損傷の激しい死体でも即死と判断された場合(例:17歳女性。電車に跳ねられた。身体の諸処に強い打撲損傷、四肢骨骨折。尿中Mb濃度2ng/ml)には尿中Mb濃度が低い傾向にあった。以上のように、本研究により、外傷死体の尿中Mb濃度測定によって、当該死体の受傷後生存時間を推定し得る可能性が示唆された。

    researchmap

  • Development of methods for typing of single locus DNA polymorphisms, Km, A2m, HLA DRB1 and alpha2-macroglobulin genotypes

    Grant number:06454247  1994 - 1995

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (B)  Grant-in-Aid for General Scientific Research (B)

    ISHIZU Hideo, NAKAMURA Chiemi, MIYAISHI Satoru, YAMAMOTO Yuji

      More details

    Grant amount:\4100000 ( Direct expense: \4100000 )

    We have developed methods for genotyping single locus DNA polymorphisms by PCR using allele-specific amplification (ASA) primers.
    1.Km genotyping
    Km (kappa marker) genotyping was performed by semi-nested PCR using ASA primers designed for discriminating base substitutions between three common alleles in a gene of the human immunoglobulin kappa light chain constant region, Km**1, Km**1,2 and Km**3, which manifest Km allotypic specificity. By this method, Km genotypes were determined in not only lymphocyte DNA but also blood, bloodstains, saliva stains and hair roots. The estimated allele frequency in 115 Japanese was 0.739 for Km**3 and 0.261 for Km**1,2.
    2.IgA2 genotyping
    IgA2 genotyping was performed by nested PCR using ASA primers which were designed for discriminating base substitutions in the 3'-flanking region of alleles, A2m**1 and A2m**2, which manifest A2m serum types. By this method, IgA2 genotyping was possible from 100 pg of lymphocyte DNA.The estimated allele frequency in 318 Japanese was 0.561 for IgA2**1 and 0.439 for IgA2**2. This genotype could be detected in whole blood, bloodstains, saliva stains, and various organs and tissues.
    3.HLA DRB1 typing
    The genetic polymorphism of the HLA DRB1 locus was examined by semi-nested PCR followed by RFLP analysis. DRB1 typing was possible from 10 pg of lymphocyte DNA by this method and the type could be determined in whole blood and bloodstains.
    4.Hp genotyping
    Hp genotyping was performed by PCR using three pairs of ASA primers which were designed for discriminating changes in the nucleotide sequences among three common Hp alleles, Hp**1S,Hp**1F and Hp**2FS.By this method, Hp genotyping was possible from 200 pg of lymphocyte DNA.Hp genotypes could also be detected from whole blood, bloodstains, salliva stains and hair roots.
    5.Alpha-2-macroglobulin genotyping
    We have not yet achieved alpha-2-macroglobulin genotyping because of the difficulty to design suitable ASA primers.

    researchmap

  • FDP-Dダイマーとミオグロビン検出に基づく月経血痕の証明法

    Grant number:05770300  1993

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    宮石 智

      More details

    Grant amount:\900000 ( Direct expense: \900000 )

    法医学実務においては、着衣等に付着した血痕が月経血に由来するか否かがしばしば問題とされる。従来の月経血痕証明法の一つである血痕からのフィブリン分解産物(FDP;月経血に多量に含まれている)の検出では、月経血は生体末梢血からは鑑別され得たが、月経血同様多量のFDPを含有する死体血との鑑別は困難であった。ところで私は、死体血と生体末梢血のミオグロビン(Mb)含量の差を利用した、血痕からの生体血と死体血との鑑別法を既に考案している。月経血は子宮という平滑筋臓器から出血する生体の血液であり、Mb含有量は生体末梢血と同程度と考えられる。そこで、血痕からのFDPとMbの同時検出によって、生体末梢血、死体血との鑑別を含めた月経血痕の証明が可能であると考えられた。
    月経血及び死体血の各10mu1を晒し布に付着させて実験的に血痕を作製し、これに200mu1のPBSを加え37℃で一夜静置して得られた抽出液についてFDP-Dダイマー(二次線溶に特異的に出現するFDPの一成分)の定量を行った結果は、月経血では5.94x10^2〜1.27x10^3ng/ml、死体血では5.84x10^2〜1.58x10^4ng/mlと高値であった。一方生体末梢血のFDP-Dダイマー濃度は9.7〜37.1ng/mlに過ぎないことから、血痕からのFDP-Dダイマー検出により月経血及び死体血と生体末梢血との鑑別が可能であった。月経血のMb濃度を測定すると27.2〜53.7ng/mlであり、生体末梢血のMbレベル(35.5±23.9ng/ml)に相当していた。従って血痕からのMb検出により、月経血も生体末梢血と同様に死体血から鑑別され得ることが判明した。
    以上のことから、血痕からのFDP-DダイマーとMbの同時検出により月経血、生体末梢血、死体血の三者が鑑別され得ること(FDP-Dダイマーのみ検出されたものは月経血、FDP-DダイマーとMbの両者が検出されたものは死体血、いずれも検出されないものは生体末梢血)が確認された。

    researchmap

  • 抗ヒトミオグロビン抗体を用いたELISAによる血痕からの生体血・死体血の鑑別法

    Grant number:04770362  1992

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    宮石 智

      More details

    Grant amount:\900000 ( Direct expense: \900000 )

    researchmap

  • Gm genotyping from forensic biological materials by DNA hybridization

    Grant number:03670297  1991 - 1992

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (C)  Grant-in-Aid for General Scientific Research (C)

    ISHIZU Hideo, MIYAISHI Satoru, MORIYA Fumio, YAMAMOTO Yuji

      More details

    Grant amount:\2000000 ( Direct expense: \2000000 )

    We have investigated the genetic polymorphisms of immunoglobulin G (IgG) and immunoglobulin kappa light chain (Ig kappa) genes by using the polymerase chain reaction (PCR).
    In the present methods, samples of extracted human genome DNA were subjected to the first PCR for the amplification of the polymorphic regions within the immunoglobulin genes.
    Small portions of the amplification products obtained from the first PCR were further subjected to the second PCR amplifications in which the allele specific primer sets were used for genotyping of the immunoglobulin genes.
    By this methods, alleles on the IgG3 constant gene, G3m*t andnon-G3m*t alleles, which determine the G3m(t) allotype and its antithetical isoallotype non-G3m(t), respectively, and alleles on the Ig kappa constant gene, Km*1, Km*1.2 and Km*3, were examined for genotyping.
    For the examination of the Ig kappa constant gene, 353bp fragments which contain the polymorphic region of the Km allotypes were successfully amplified from sample DNAs by the first PCR. The second PCRs using the products of first PCR as samples could clearly identify the sequence changes between the three alleles. Results of a tiny population study among 72 individuals living in Okayama Prefecture showed the gene frequencies of Km*3= 0.736 and Km*1.2= 0.264.
    In the case of the examination of the IgG3 constant gene, 726bp fragments which contain the polymorphic region of the G3m(t) allotype were successfully amplified from sample DNAs by the first PCR. Using the products of first PCR as samples, the second PCRs could generally distinguish the nucleotide substision between the two alleles.

    researchmap

▼display all

 

Class subject in charge

  • Legal Medicine (2021academic year) special  - その他

  • Practice in Legal Medicine (2021academic year) special  - その他

  • Research Projects and Practicals: Legal Medicine I (2021academic year) special  - その他

  • Lecture and Research Projects: Legal Medicine I (2021academic year) special  - その他

  • Research Projects and Practicals: Legal Medicine II (2021academic year) special  - その他

  • Lecture and Research Projects: Legal Medicine II (2021academic year) special  - その他

  • Research Presentation in Pathophysiology (2021academic year) special  - その他

  • Elective Clinical Practice (Legal Medicine) (2021academic year) special  - その他

  • Legal Medicine (2020academic year) special  - その他

  • Practice in Legal Medicine (2020academic year) special  - その他

  • Research Projects and Practicals: Legal Medicine I (2020academic year) special  - その他

  • Lecture and Research Projects: Legal Medicine I (2020academic year) special  - その他

  • Research Projects and Practicals: Legal Medicine II (2020academic year) special  - その他

  • Lecture and Research Projects: Legal Medicine II (2020academic year) special  - その他

  • Research Presentation in Pathophysiology (2020academic year) Year-round  - その他

  • Pathophysiology (2020academic year) Year-round  - その他

  • Elective Clinical Practice (Legal Medicine) (2020academic year) special  - その他

▼display all

 

Social Activities